FastQCFastQC Report
Thu 26 May 2016
SRR938371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201546
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66790.30337771729502816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.24046738064977974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.16293095851733283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.056.2279661
GTATCAA69600.043.6674731
ATCAACG91550.032.884563
TATCAAC92600.032.511682
TCAACGC93200.032.0982554
CAACGCA94800.031.6066845
AACGCAG98350.030.4422686
TGGTATC16950.027.2175312
GTGGTAT19000.026.5349791
ACGCAGA113600.026.1918417
GTACATG96000.025.8126721
CGCAGAG115400.025.7421198
TACATGG98200.024.6520482
ACATGGG100000.023.5424633
GCAGAGT125600.023.0072259
GAGTACT66650.021.3190112-13
AACCGGT1353.784664E-421.1216746
GTACTTT70800.020.12917714-15
AGAGTAC118950.020.1168810-11
AGTACTT70550.019.87105812-13
Produced by FastQC (version 0.11.5)