##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938371_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2201546 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28112517294665 31.0 31.0 34.0 28.0 34.0 2 31.32578151898711 31.0 31.0 34.0 28.0 34.0 3 31.369665680390053 31.0 31.0 34.0 28.0 34.0 4 35.0386492037868 37.0 35.0 37.0 32.0 37.0 5 35.02269586917557 37.0 35.0 37.0 32.0 37.0 6 35.04825245532003 37.0 35.0 37.0 32.0 37.0 7 34.96113095070464 37.0 35.0 37.0 32.0 37.0 8 34.93594819276999 37.0 35.0 37.0 32.0 37.0 9 36.45239572554923 39.0 37.0 39.0 32.0 39.0 10-11 36.290050946017026 39.0 35.0 39.0 32.0 39.0 12-13 36.151662513524585 39.0 35.0 39.0 32.0 39.0 14-15 37.132348585948236 39.0 36.0 41.0 31.0 41.0 16-17 36.96032197374027 39.0 36.0 41.0 31.0 41.0 18-19 37.120191901509216 39.0 36.0 41.0 31.0 41.0 20-21 37.1184260969337 39.0 36.0 41.0 31.0 41.0 22-23 37.028144313132685 39.0 36.0 41.0 31.0 41.0 24-25 36.89293841691248 39.0 36.0 41.0 30.5 41.0 26-27 36.69902218713577 39.0 36.0 41.0 30.0 41.0 28-29 36.49539482708969 39.0 36.0 40.5 30.0 41.0 30-31 36.245621031765864 39.0 35.0 40.0 29.5 41.0 32-33 35.9941668264029 38.0 35.0 40.0 29.0 41.0 34-35 35.7422847399055 38.0 35.0 40.0 27.5 41.0 36-37 35.474675977699306 38.0 34.5 40.0 27.0 41.0 38-39 35.35537027161822 38.0 34.0 40.0 26.5 41.0 40-41 35.25639028210176 38.0 34.0 40.0 26.0 41.0 42-43 34.97254134140282 38.0 34.0 40.0 25.0 41.0 44-45 34.6296965859446 38.0 33.0 40.0 24.0 41.0 46-47 34.24710748719309 38.0 33.0 40.0 23.5 41.0 48-49 33.89033388355274 38.0 33.0 40.0 22.5 41.0 50-51 33.21247636887896 37.5 32.0 39.5 21.0 40.5 52-53 33.24928391230526 37.0 32.5 39.5 21.0 40.0 54-55 33.355327801463154 37.0 32.5 40.0 20.0 41.0 56-57 33.04834761572096 37.0 32.0 40.0 18.5 41.0 58-59 32.6958085363649 37.0 31.0 39.5 16.0 41.0 60-61 32.22695642062442 36.0 31.0 39.0 12.5 41.0 62-63 31.658907422329584 35.5 30.5 39.0 8.0 40.0 64-65 31.146177277240632 35.0 29.5 38.5 7.5 40.0 66-67 30.61406938578617 35.0 29.0 38.0 3.5 40.0 68-69 30.024555925699488 34.5 28.5 38.0 2.0 40.0 70-71 29.3810204283717 34.0 27.5 37.0 2.0 39.0 72-73 28.73577590475057 34.0 26.0 36.5 2.0 39.0 74-75 28.0446724710726 33.5 26.0 36.0 2.0 39.0 76-77 27.27679389846953 32.5 25.0 35.0 2.0 37.5 78-79 26.478811480659502 32.0 23.0 35.0 2.0 37.0 80-81 25.75396516811368 31.5 20.5 35.0 2.0 36.5 82-83 24.99975857874421 31.0 18.5 34.5 2.0 36.0 84-85 24.265902915496653 31.0 14.0 34.0 2.0 35.5 86-87 23.46248318227282 30.0 7.0 34.0 2.0 35.0 88-89 22.621767839509147 29.5 2.0 34.0 2.0 35.0 90-91 21.67733515447781 29.0 2.0 34.0 2.0 35.0 92-93 20.67574490835077 28.0 2.0 34.0 2.0 35.0 94-95 19.611048099835298 26.5 2.0 33.0 2.0 35.0 96-97 18.29988698850717 24.5 2.0 32.5 2.0 34.0 98-99 16.727363180237887 11.0 2.0 32.0 2.0 34.0 100-101 13.140004796629277 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2072864320429879 1101 2 0.21500817483853396 1101 3 0.11998399879598409 1101 4 0.09458313784529793 1101 5 -0.047871845488351994 1101 6 0.28381722605021764 1101 7 0.12557657182548354 1101 8 0.11674928399346385 1101 9 0.3074866410103212 1101 10-11 0.2467498059848836 1101 12-13 0.2736229423542724 1101 14-15 0.4721496909966092 1101 16-17 0.3048280474489715 1101 18-19 0.35470771887234775 1101 20-21 0.3506655910702179 1101 22-23 0.31692520330563667 1101 24-25 0.4620101387424711 1101 26-27 0.30129525716881034 1101 28-29 0.3398035888374906 1101 30-31 0.29608767435206573 1101 32-33 0.3417848954130349 1101 34-35 0.2730147595801995 1101 36-37 0.37181701466927564 1101 38-39 0.2956570322646712 1101 40-41 0.2126596626140227 1101 42-43 0.3658820151300475 1101 44-45 0.466624175196074 1101 46-47 0.36492029445747676 1101 48-49 0.27699652317460277 1101 50-51 0.4444696805603172 1101 52-53 0.36349869955854786 1101 54-55 0.4204394427976723 1101 56-57 0.4969990873942791 1101 58-59 0.3869696897376329 1101 60-61 0.41900169513460384 1101 62-63 0.5432368977398561 1101 64-65 0.36752704908924017 1101 66-67 0.37354108157441246 1101 68-69 0.3361858888626479 1101 70-71 0.6569858376165847 1101 72-73 0.752103610996258 1101 74-75 0.5280527335930039 1101 76-77 0.614496173059262 1101 78-79 0.6231932485682705 1101 80-81 0.8247125995972979 1101 82-83 0.7902980627364613 1101 84-85 0.46372341553571417 1101 86-87 0.6413614363418425 1101 88-89 0.8188830425145319 1101 90-91 0.5798337989939633 1101 92-93 0.7531501191323748 1101 94-95 1.0531421789171596 1101 96-97 0.921183917892705 1101 98-99 0.8450051892729036 1101 100-101 0.9515158331627536 1102 1 0.5045054448864441 1102 2 0.42432924717040166 1102 3 0.4302110486977142 1102 4 0.29504694662408326 1102 5 0.2400573113273552 1102 6 0.41071170955304126 1102 7 0.31277697510862623 1102 8 0.45698253984568993 1102 9 0.6807851347925578 1102 10-11 0.6370836753432059 1102 12-13 0.6318733534933045 1102 14-15 0.7661800184859047 1102 16-17 0.8177771134679261 1102 18-19 0.7828922897269308 1102 20-21 0.6713050195423946 1102 22-23 0.7118701104671814 1102 24-25 0.662373727846358 1102 26-27 0.6474751567708736 1102 28-29 0.5638354999427477 1102 30-31 0.6301272092001113 1102 32-33 0.5184148694491952 1102 34-35 0.5009802151952201 1102 36-37 0.6921019950668423 1102 38-39 0.5723126675123069 1102 40-41 0.7265302936269435 1102 42-43 0.7470104948877463 1102 44-45 0.8414405773118219 1102 46-47 0.9488864523356995 1102 48-49 0.8522271942505171 1102 50-51 0.7595273809758254 1102 52-53 0.6974239197205492 1102 54-55 0.7723182870565068 1102 56-57 0.8750288411337053 1102 58-59 0.9159488253900889 1102 60-61 0.855150569129755 1102 62-63 0.9142770805438651 1102 64-65 1.0927938282672613 1102 66-67 0.9200850884793113 1102 68-69 0.7954092288745294 1102 70-71 0.9631039844874323 1102 72-73 1.1443285955642963 1102 74-75 1.265500297042319 1102 76-77 1.0241861201242024 1102 78-79 0.9036288341600205 1102 80-81 1.133590259460636 1102 82-83 1.2747975924191017 1102 84-85 1.3824036996413902 1102 86-87 1.322731790636528 1102 88-89 1.2596520746142588 1102 90-91 1.274978295659043 1102 92-93 1.2961721351252358 1102 94-95 1.4065441134988994 1102 96-97 1.684037324387436 1102 98-99 1.60874461231435 1102 100-101 1.5674717588919957 1103 1 0.3830405767139382 1103 2 0.319519073440123 1103 3 0.3004121820682961 1103 4 0.26344692534012637 1103 5 0.24237194138561335 1103 6 0.325629101204413 1103 7 0.26751531549226826 1103 8 0.35935570077899115 1103 9 0.3915960534612424 1103 10-11 0.5488849630224593 1103 12-13 0.5670903168050927 1103 14-15 0.7310049579596622 1103 16-17 0.7325215729885102 1103 18-19 0.7275240773130633 1103 20-21 0.663336839054459 1103 22-23 0.7004665666887533 1103 24-25 0.710093019090678 1103 26-27 0.7685110202343068 1103 28-29 0.7709416802710507 1103 30-31 0.886801724325025 1103 32-33 1.02151767094972 1103 34-35 0.9392966543896222 1103 36-37 0.9154937262504959 1103 38-39 0.9063277525151037 1103 40-41 0.966254134316543 1103 42-43 1.0871979267125838 1103 44-45 0.7557028222369269 1103 46-47 0.7102434374635322 1103 48-49 0.7422490103039365 1103 50-51 1.0087059533545784 1103 52-53 0.9440450919343419 1103 54-55 0.9986365619919866 1103 56-57 1.0424470974457307 1103 58-59 0.9635650224795071 1103 60-61 1.069689505464133 1103 62-63 1.1631264167505257 1103 64-65 1.1345536122351234 1103 66-67 1.07519523294415 1103 68-69 1.3758126928381316 1103 70-71 1.3230422610192107 1103 72-73 1.3341382371957096 1103 74-75 1.1249131259459304 1103 76-77 1.3269803042836195 1103 78-79 1.4132797773052523 1103 80-81 1.5699346072404552 1103 82-83 1.5501121958724227 1103 84-85 1.708038227536676 1103 86-87 1.7611845801648265 1103 88-89 1.7244703628522124 1103 90-91 1.8245599329272473 1103 92-93 1.6534366148655728 1103 94-95 2.0013342004970625 1103 96-97 2.104638197029576 1103 98-99 1.8417540304642408 1103 100-101 1.6672170165608708 1104 1 0.3573837390749155 1104 2 0.2756838697043378 1104 3 0.3516207256649899 1104 4 0.21398717665191924 1104 5 0.2593012803427115 1104 6 0.2511213371512895 1104 7 0.29920467177255716 1104 8 0.411507291248725 1104 9 0.49228003704187273 1104 10-11 0.539239131258249 1104 12-13 0.4503584528046929 1104 14-15 0.4387231925005963 1104 16-17 0.5509234615928591 1104 18-19 0.5616945412732974 1104 20-21 0.6823123229740204 1104 22-23 0.6840950532946621 1104 24-25 0.6950776259295708 1104 26-27 0.7310068620409353 1104 28-29 0.707588466066035 1104 30-31 0.7635783712834154 1104 32-33 0.6330078576133715 1104 34-35 0.6295948046996855 1104 36-37 0.9147232836304227 1104 38-39 0.791767171560565 1104 40-41 0.7345242944932906 1104 42-43 0.8643909422030234 1104 44-45 0.7562249501021583 1104 46-47 0.6856300709901859 1104 48-49 0.6288990128145429 1104 50-51 0.5236393594955189 1104 52-53 0.6269879473735784 1104 54-55 0.8067673206975172 1104 56-57 0.984388910982922 1104 58-59 0.935393654700647 1104 60-61 0.9454521024363416 1104 62-63 1.0419511700833617 1104 64-65 1.130402479951222 1104 66-67 1.1204038173382145 1104 68-69 1.2176036345669345 1104 70-71 1.3479910289386723 1104 72-73 1.2731218492064542 1104 74-75 1.2135218729018185 1104 76-77 1.1921689293495241 1104 78-79 1.3641836743954592 1104 80-81 1.2554900634178026 1104 82-83 1.4601857723580736 1104 84-85 1.5110869678394572 1104 86-87 1.531294152482868 1104 88-89 1.591787512183135 1104 90-91 1.3004855624620326 1104 92-93 1.275262658022914 1104 94-95 1.4691807741457268 1104 96-97 1.5166975069891535 1104 98-99 1.7621905309287023 1104 100-101 1.4906742483652433 1105 1 0.30098917218398924 1105 2 0.2339245943585162 1105 3 0.216071199242311 1105 4 0.22771953357525376 1105 5 0.2105555413404261 1105 6 0.31912525067924236 1105 7 0.2615878874853763 1105 8 0.20422809929564778 1105 9 0.4869457396796477 1105 10-11 0.45336420764169105 1105 12-13 0.33725676473069655 1105 14-15 0.4528047041138805 1105 16-17 0.4688233401282176 1105 18-19 0.479372477243011 1105 20-21 0.44776012462869375 1105 22-23 0.6448446369300385 1105 24-25 0.6873017731309403 1105 26-27 0.628716238782026 1105 28-29 0.4563503242204945 1105 30-31 0.5506364144595324 1105 32-33 0.5256300598827082 1105 34-35 0.7094665142209493 1105 36-37 0.7746296914520912 1105 38-39 0.7702513796590154 1105 40-41 0.877576808856368 1105 42-43 0.8427309952820323 1105 44-45 0.8267353824324175 1105 46-47 0.7040894020509896 1105 48-49 0.6063518572739 1105 50-51 0.7082847156819767 1105 52-53 0.6224591395842651 1105 54-55 0.5322289537454878 1105 56-57 0.3939133828887904 1105 58-59 0.46641225781229423 1105 60-61 0.6572341729966595 1105 62-63 0.7183128546527051 1105 64-65 0.8723299860768741 1105 66-67 0.9076584355408386 1105 68-69 0.8009394640910834 1105 70-71 0.8581738961333301 1105 72-73 0.8018901363003508 1105 74-75 0.8128899871594584 1105 76-77 0.9305808315367834 1105 78-79 0.9175035270503109 1105 80-81 0.9406928502151501 1105 82-83 0.947694178280635 1105 84-85 1.022013450277253 1105 86-87 1.19718016667521 1105 88-89 1.1068649103235764 1105 90-91 1.0003958119213543 1105 92-93 0.852301815019338 1105 94-95 1.2164155251656688 1105 96-97 1.4513184317080352 1105 98-99 1.7271112494122995 1105 100-101 1.2852252635161232 1106 1 0.2991812919148096 1106 2 0.24929611347606695 1106 3 0.18839228011139042 1106 4 0.09895060874644912 1106 5 0.1908849596059241 1106 6 0.002398071348373776 1106 7 0.3030571017639119 1106 8 0.19513351528865996 1106 9 0.24096421327909212 1106 10-11 0.19804671938606333 1106 12-13 0.11135793084402223 1106 14-15 0.39291022150965205 1106 16-17 0.2459580711877365 1106 18-19 0.16873576007063207 1106 20-21 0.2978438337627338 1106 22-23 0.30591634346493635 1106 24-25 0.07752734984993026 1106 26-27 0.011606039933717227 1106 28-29 0.27303694985076987 1106 30-31 0.010828976021997505 1106 32-33 0.27579658004372476 1106 34-35 0.006192015850785992 1106 36-37 0.1020837875332461 1106 38-39 -0.0920774175237824 1106 40-41 0.14000591626571435 1106 42-43 -0.22902238933168206 1106 44-45 0.11243944374665915 1106 46-47 0.18781755374479303 1106 48-49 0.6468271355343234 1106 50-51 0.38613491401549993 1106 52-53 0.08175183489986182 1106 54-55 0.25534365572115547 1106 56-57 -0.4236209817430492 1106 58-59 -0.15227370229286663 1106 60-61 0.47360655374146887 1106 62-63 0.4891038018075271 1106 64-65 0.48539379593594134 1106 66-67 0.02072724517121216 1106 68-69 0.2446260213911451 1106 70-71 0.0922556890587316 1106 72-73 0.3445267971790109 1106 74-75 0.7479137925520689 1106 76-77 0.7294859556912918 1106 78-79 0.7987288672986388 1106 80-81 0.8346097395722509 1106 82-83 0.5382942934235189 1106 84-85 0.6332810460001923 1106 86-87 0.4496215827808072 1106 88-89 0.46843550144653534 1106 90-91 0.5404558313982442 1106 92-93 0.4204822149158609 1106 94-95 0.2175356591151001 1106 96-97 0.2687425236060541 1106 98-99 0.36351815820739475 1106 100-101 0.009665645594942873 1107 1 0.16842810260120444 1107 2 0.06075120587475524 1107 3 0.09605376935485666 1107 4 -0.04652927753040359 1107 5 0.04779885873340817 1107 6 0.19188743076311 1107 7 0.032938379327617895 1107 8 -0.05682927683232464 1107 9 0.33508632165219154 1107 10-11 0.2698819297898538 1107 12-13 0.3191641149486486 1107 14-15 0.4865618468455395 1107 16-17 0.46115429719645107 1107 18-19 0.5515089810090572 1107 20-21 0.4354680567869238 1107 22-23 0.4306635127238039 1107 24-25 0.4668380743130882 1107 26-27 0.34173029185284776 1107 28-29 0.45228887723511235 1107 30-31 0.6012336354643821 1107 32-33 0.6658111485982161 1107 34-35 0.6545998311711969 1107 36-37 0.5947076885852312 1107 38-39 0.4447837221917865 1107 40-41 0.43621025835908256 1107 42-43 0.5367109982324152 1107 44-45 0.6064347079115535 1107 46-47 0.5390559359142486 1107 48-49 0.5463919868831439 1107 50-51 0.4121329315473048 1107 52-53 0.648696461734076 1107 54-55 0.5195252473132612 1107 56-57 0.508694497451593 1107 58-59 0.607073994853657 1107 60-61 0.48929109509435165 1107 62-63 0.6965077499756909 1107 64-65 0.5667348918266057 1107 66-67 0.44024088917929305 1107 68-69 0.7484019940453415 1107 70-71 0.8427684137490061 1107 72-73 0.84016329404065 1107 74-75 0.753670582267155 1107 76-77 0.6615989868330665 1107 78-79 0.8685356527849741 1107 80-81 0.9390134938232464 1107 82-83 0.9402775138097326 1107 84-85 1.1126488650338295 1107 86-87 1.2844203024505205 1107 88-89 1.2626716852154303 1107 90-91 1.4268805474160011 1107 92-93 1.5933747783852077 1107 94-95 1.7645214947228745 1107 96-97 1.719195403661221 1107 98-99 1.8450258540770328 1107 100-101 1.7131343778738586 1108 1 0.36399606965057174 1108 2 0.2290655703329918 1108 3 0.200551200762618 1108 4 0.21190870164065956 1108 5 0.1786411808936279 1108 6 0.2777443637471322 1108 7 0.17011731914166006 1108 8 0.353458825244644 1108 9 0.4128933192317277 1108 10-11 0.2922411694400395 1108 12-13 0.4481854140244863 1108 14-15 0.4892960118080296 1108 16-17 0.5274883232772041 1108 18-19 0.6138997182396082 1108 20-21 0.3969795973165233 1108 22-23 0.4308941516517777 1108 24-25 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-2.310755669374494 2316 76-77 -2.575360905810303 2316 78-79 -2.5801692351329066 2316 80-81 -2.5930523439389574 2316 82-83 -2.6001612409515573 2316 84-85 -2.6513281580290275 2316 86-87 -2.958431112537678 2316 88-89 -3.0024979619887553 2316 90-91 -2.9700382682243536 2316 92-93 -3.137221042725262 2316 94-95 -3.371556306102754 2316 96-97 -3.4409680860838474 2316 98-99 -3.510406825051838 2316 100-101 -3.1869204460146303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7269.0 3 2239.0 4 2341.0 5 2678.0 6 3195.0 7 3940.0 8 5023.0 9 6447.0 10 8147.0 11 10121.0 12 12728.0 13 15258.0 14 17825.0 15 20271.0 16 24020.0 17 27782.0 18 26639.0 19 25744.0 20 27250.0 21 30088.0 22 32959.0 23 37465.0 24 41759.0 25 47300.0 26 53119.0 27 61030.0 28 69615.0 29 80492.0 30 92845.0 31 108713.0 32 128487.0 33 152895.0 34 184441.0 35 222099.0 36 256948.0 37 243649.0 38 104477.0 39 4248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.479578213672305 18.696928634927154 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22.820669698447812 44-45 22.494206457922747 26.630465025585163 28.195215396206052 22.680113120286038 46-47 22.690362720804675 26.735585444285114 27.7120130326632 22.86203880224701 48-49 22.713631208705447 26.81795974214732 27.664378130629398 22.804030918517835 50-51 22.74235747555875 26.914296473914533 27.512633721890268 22.830712328636444 52-53 23.025415309331578 26.789649440719643 27.4423741125175 22.742561137431284 54-55 22.54851366496054 26.757415093988197 27.70915378140152 22.984917459649743 56-57 22.63415717800642 27.102466627658067 27.494702947087617 22.768673247247897 58-59 22.72882360049902 26.562237347951438 27.587426502858108 23.12151254869143 60-61 22.608782767043223 26.725063062473648 27.804871621523386 22.861282548959743 62-63 22.64565750871987 26.737023768746926 27.765964019344985 22.851354703188214 64-65 22.34211381655859 26.59631710768961 27.792679051696002 23.268890024055796 66-67 22.362660750213852 26.907663087448935 27.581350090559344 23.148326071777873 68-69 22.575752138177098 26.54885246884669 27.78662612076277 23.08876927221344 70-71 22.36215313370119 26.735194797641636 27.59724887861406 23.305403190043112 72-73 22.633106789852526 26.650777726586643 27.500361215537993 23.215754268022838 74-75 22.49700970864964 26.83658412743128 27.37886906215603 23.28753710176305 76-77 22.594862160654877 26.708859177950252 27.427275277740232 23.269003383654642 78-79 22.665655501605553 26.853654980124205 27.325145894401842 23.155543623868404 80-81 22.550759121242724 27.104623501029106 27.163835094236603 23.18078228349157 82-83 22.758703239267135 27.016961532992973 27.082579076066835 23.141756151673054 84-85 22.77283919380566 26.668285311884848 27.125056696114957 23.433818798194537 86-87 22.83845345912864 26.707077076276732 27.14185316693076 23.312616297663872 88-89 22.746061379789978 26.675059544217216 27.107040542778797 23.471838533214008 90-91 22.694841457729183 26.804849100619883 27.136192495426535 23.364116946224403 92-93 22.95051837514071 26.521405763863566 27.101222586093893 23.42685327490183 94-95 22.72036203084288 26.484003116599116 27.09898383326695 23.696651019291057 96-97 23.02042214846882 26.25391573526301 27.060908811849988 23.66475330441818 98-99 22.828459931820692 26.318071969471518 26.794909132874228 24.05855896583356 100-101 23.453352492032487 25.813286324516298 27.085792757848075 23.64756842560314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 484.0 1 346.5 2 272.0 3 347.5 4 317.0 5 304.0 6 511.0 7 1133.0 8 1809.0 9 2043.0 10 1796.5 11 1482.0 12 1494.5 13 1679.5 14 1794.5 15 1944.5 16 2151.0 17 2301.0 18 2489.0 19 2753.5 20 3167.5 21 3708.5 22 4309.0 23 5130.5 24 6177.0 25 7720.0 26 9851.5 27 12599.5 28 15289.5 29 18231.0 30 21817.0 31 25686.5 32 30513.0 33 34678.5 34 38306.5 35 42319.5 36 46877.0 37 51856.5 38 55974.5 39 59150.0 40 62528.5 41 67546.5 42 72114.0 43 76569.0 44 80265.5 45 82735.0 46 83784.0 47 85195.5 48 89102.5 49 90117.0 50 88548.0 51 85636.5 52 81664.0 53 79821.0 54 77633.0 55 71635.0 56 64065.0 57 57759.0 58 52338.0 59 45620.0 60 38282.0 61 31881.0 62 26049.0 63 20409.5 64 15927.5 65 12581.5 66 9601.5 67 7406.5 68 5563.0 69 4002.5 70 3117.0 71 2519.5 72 2110.5 73 1771.5 74 1390.0 75 1134.5 76 862.0 77 578.5 78 383.0 79 249.0 80 171.0 81 108.0 82 60.0 83 44.5 84 30.0 85 20.5 86 14.0 87 8.0 88 4.0 89 2.5 90 1.0 91 0.5 92 2.0 93 3.0 94 2.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006677125983286291 2 0.1065614799781608 3 0.11691783864611505 4 0.11882558892705399 5 0.1582070054407221 6 0.0975678000823058 7 0.028343718459664255 8 0.028525408962610823 9 0.02539124778678256 10-11 0.05355327574350025 12-13 0.055597293901649115 14-15 0.029025057845713877 16-17 0.03347647516790474 18-19 0.038586520563276897 20-21 0.06000328859810333 22-23 0.03324936203922153 24-25 0.050578093757750235 26-27 0.058527053261662486 28-29 0.05523391289575598 30-31 0.069610173941403 32-33 0.051372989708141456 34-35 0.0641367475401377 36-37 0.05443901694536476 38-39 0.03293140365906504 40-41 0.03860923187614522 42-43 0.034044257989612754 44-45 0.007835402939570647 46-47 0.01396745741401724 48-49 0.009606885343299662 50-51 0.0088801233315134 52-53 0.010674317048110737 54-55 0.011764460065790131 56-57 0.01462608548719854 58-59 0.01998595532412223 60-61 0.025164134658099354 62-63 0.01144650168563364 64-65 0.009334349588879813 66-67 0.010310936042217606 68-69 0.011582769562843567 70-71 0.031704992764175724 72-73 0.02925217097439708 74-75 0.030592138433628006 76-77 0.02377874457313179 78-79 0.005677828217080179 80-81 0.005405292462660331 82-83 0.0069496617377061394 84-85 0.005291735898318726 86-87 0.013535942469519146 88-89 0.039540395703746366 90-91 0.03670148159520628 92-93 0.030819251562311214 94-95 0.04862492085107466 96-97 0.04403723565167387 98-99 0.026572236055935238 100-101 0.07608289810887439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2201546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.096604248909216 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84418293290973 41.02912748703493 2 13.228324306636296 14.312148497846605 3 4.65532643116958 7.555120547894031 4 2.121641993425714 4.590945091048709 5 1.1257506440292178 3.044964353650164 6 0.6975952321942406 2.2642519921162663 7 0.43797094657957064 1.6584918678744638 8 0.31041238272517613 1.343380465779584 9 0.22903791069330248 1.115115589149534 >10 1.1966213995983486 11.808071010269131 >50 0.09424844362515439 3.559432513367184 >100 0.05417281138799722 5.25624721629153 >500 0.0037884897529096716 1.3451186145720326 >1k 7.576979505818938E-4 0.5722959373865399 >5k 1.6837732235153193E-4 0.5452888157193253 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6679 0.30337771729502816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5294 0.24046738064977974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3587 0.16293095851733283 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.542262573664143E-5 10-11 0.0 0.0 0.0 0.0 4.542262573664143E-5 12-13 0.0 0.0 0.0 0.0 4.542262573664143E-5 14-15 0.0 0.0 0.0 4.542262573664143E-5 4.542262573664143E-5 16-17 0.0 0.0 0.0 9.084525147328286E-5 4.542262573664143E-5 18-19 0.0 0.0 0.0 1.362678772099243E-4 4.542262573664143E-5 20-21 0.0 0.0 0.0 1.58979190078245E-4 4.542262573664143E-5 22-23 0.0 0.0 0.0 5.677828217080179E-4 4.542262573664143E-5 24-25 0.0 0.0 0.0 0.0010901430176793944 4.542262573664143E-5 26-27 0.0 0.0 0.0 0.001839616342333978 4.542262573664143E-5 28-29 0.0 0.0 0.0 0.004655819138005747 4.542262573664143E-5 30-31 0.0 0.0 0.0 0.01489862124161839 4.542262573664143E-5 32-33 0.0 0.0 0.0 0.0343167937440326 4.542262573664143E-5 34-35 0.0 0.0 0.0 0.06368252128277128 4.542262573664143E-5 36-37 0.0 0.0 0.0 0.11292064758129061 4.542262573664143E-5 38-39 0.0 0.0 0.0 0.18398434554626614 4.542262573664143E-5 40-41 0.0 0.0 0.0 0.26556338136927415 4.542262573664143E-5 42-43 0.0 0.0 0.0 0.34739224163383364 4.542262573664143E-5 44-45 0.0 0.0 0.0 0.44879825359088565 4.542262573664143E-5 46-47 0.0 0.0 0.0 0.5707807150066362 4.542262573664143E-5 48-49 0.0 0.0 0.0 0.7055723568801198 4.542262573664143E-5 50-51 0.0 0.0 0.0 0.8485854940119353 4.542262573664143E-5 52-53 0.0 0.0 0.0 0.9917803216466974 4.542262573664143E-5 54-55 0.0 0.0 0.0 1.1502598628418395 9.084525147328286E-5 56-57 0.0 0.0 0.0 1.3350845269642333 9.084525147328286E-5 58-59 0.0 0.0 0.0 1.5388050033930702 9.084525147328286E-5 60-61 0.0 0.0 0.0 1.750179192258531 9.084525147328286E-5 62-63 0.0 0.0 0.0 1.9620303186942267 9.084525147328286E-5 64-65 0.0 0.0 0.0 2.192482010369077 9.084525147328286E-5 66-67 0.0 0.0 0.0 2.451231997877855 9.084525147328286E-5 68-69 0.0 0.0 0.0 2.7396429599926595 9.084525147328286E-5 70-71 0.0 0.0 0.0 3.0501293182154723 9.084525147328286E-5 72-73 0.0 0.0 0.0 3.361887509958911 9.084525147328286E-5 74-75 0.0 4.542262573664143E-5 0.0 3.6815946612062613 9.084525147328286E-5 76-77 0.0 4.542262573664143E-5 0.0 4.026852039430473 9.084525147328286E-5 78-79 0.0 4.542262573664143E-5 0.0 4.403996100921807 9.084525147328286E-5 80-81 0.0 4.542262573664143E-5 0.0 4.809801839252961 9.084525147328286E-5 82-83 0.0 4.542262573664143E-5 0.0 5.2283940467289804 9.084525147328286E-5 84-85 0.0 4.542262573664143E-5 0.0 5.653481689685339 9.084525147328286E-5 86-87 0.0 4.542262573664143E-5 0.0 6.081953318259078 9.084525147328286E-5 88-89 0.0 4.542262573664143E-5 0.0 6.552191051197658 9.084525147328286E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3705 0.0 56.227966 1 GTATCAA 6960 0.0 43.667473 1 ATCAACG 9155 0.0 32.88456 3 TATCAAC 9260 0.0 32.51168 2 TCAACGC 9320 0.0 32.098255 4 CAACGCA 9480 0.0 31.606684 5 AACGCAG 9835 0.0 30.442268 6 TGGTATC 1695 0.0 27.217531 2 GTGGTAT 1900 0.0 26.534979 1 ACGCAGA 11360 0.0 26.191841 7 GTACATG 9600 0.0 25.812672 1 CGCAGAG 11540 0.0 25.742119 8 TACATGG 9820 0.0 24.652048 2 ACATGGG 10000 0.0 23.542463 3 GCAGAGT 12560 0.0 23.007225 9 GAGTACT 6665 0.0 21.31901 12-13 AACCGGT 135 3.784664E-4 21.121674 6 GTACTTT 7080 0.0 20.129177 14-15 AGAGTAC 11895 0.0 20.11688 10-11 AGTACTT 7055 0.0 19.871058 12-13 >>END_MODULE