FastQCFastQC Report
Thu 26 May 2016
SRR938371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201546
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.29656432343453193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.2456909826094935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38230.1736506981911802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36850.057.0095331
GTATCAA68150.042.4033781
ATCAACG89150.032.2388533
TATCAAC91150.031.5472432
TCAACGC92250.031.2069854
CAACGCA94150.030.5267525
AACGCAG97000.029.7129296
TGGTATC15950.027.41512
GTGGTAT17950.025.9492231
ACGCAGA114350.025.0801017
CGCAGAG115450.024.8411398
GTACATG94200.023.9160861
TACATGG93900.023.5369432
ACATGGG95850.022.6500743
GCAGAGT126150.022.3577299
GAGTACT67050.020.92604612-13
GTCTACG1604.968179E-520.794131
AGTACTT71400.020.28290412-13
AGAGTAC120850.020.15576210-11
CGCGGAA2950.020.13959382-83
Produced by FastQC (version 0.11.5)