##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938371_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2201546 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.722217478081312 31.0 31.0 34.0 30.0 34.0 2 31.950250869161945 31.0 31.0 34.0 30.0 34.0 3 32.0094865153851 33.0 31.0 34.0 30.0 34.0 4 35.699815039068 37.0 35.0 37.0 33.0 37.0 5 35.593616940095735 37.0 35.0 37.0 33.0 37.0 6 35.59617377969845 37.0 35.0 37.0 33.0 37.0 7 35.5322523354043 37.0 35.0 37.0 33.0 37.0 8 35.51134021274141 37.0 35.0 37.0 33.0 37.0 9 37.08382382198691 39.0 37.0 39.0 33.0 39.0 10-11 36.97448838225502 39.0 37.0 39.0 33.0 39.0 12-13 36.85671682535818 39.0 37.0 39.0 32.5 39.0 14-15 37.867239657949455 40.0 37.0 41.0 32.5 41.0 16-17 37.76846974807703 39.5 37.0 41.0 32.0 41.0 18-19 38.01801960985598 40.0 37.0 41.0 33.0 41.0 20-21 37.99754899511525 40.0 37.0 41.0 33.0 41.0 22-23 37.87517271953436 40.0 37.0 41.0 32.5 41.0 24-25 37.74262450114601 40.0 37.0 41.0 32.0 41.0 26-27 37.54703308493213 39.0 37.0 41.0 32.0 41.0 28-29 37.423391562111355 39.0 36.0 41.0 31.5 41.0 30-31 37.1698756237662 39.0 36.0 41.0 31.0 41.0 32-33 36.98087275941543 39.0 36.0 40.0 31.0 41.0 34-35 36.875599465103164 39.0 35.5 40.0 30.0 41.0 36-37 36.83338231406475 39.0 35.0 41.0 30.0 41.0 38-39 36.67193622118275 39.0 35.0 40.0 30.0 41.0 40-41 36.49937725580115 39.0 35.0 40.0 30.0 41.0 42-43 36.28441922176507 39.0 35.0 40.0 30.0 41.0 44-45 35.97484426852766 38.0 35.0 40.0 29.0 41.0 46-47 35.58024451907887 38.0 34.5 40.0 27.5 41.0 48-49 35.22818873646065 38.0 34.0 40.0 27.0 41.0 50-51 34.84619172163562 38.0 33.5 40.0 26.0 41.0 52-53 34.34546859343389 38.0 33.0 40.0 24.0 41.0 54-55 33.984940809776404 37.0 33.0 40.0 23.5 41.0 56-57 33.631881414242535 37.0 32.0 40.0 23.0 41.0 58-59 33.136322157247676 36.5 31.5 39.0 22.0 40.0 60-61 32.71912714973932 36.0 31.0 39.0 20.5 40.0 62-63 32.220937241374926 35.5 31.0 39.0 18.5 40.0 64-65 31.665617706829657 35.0 30.0 38.0 17.5 40.0 66-67 31.133948370826683 34.5 29.0 38.0 13.5 40.0 68-69 30.60951099818037 34.0 29.0 37.5 10.0 39.5 70-71 29.99848356563978 34.0 28.0 37.0 8.0 39.0 72-73 29.462551089098298 34.0 28.0 36.0 7.0 39.0 74-75 28.835350930664177 33.0 26.5 36.0 4.0 38.5 76-77 27.645236120435367 31.5 26.0 34.5 2.0 36.5 78-79 28.331380993174797 33.0 26.5 35.0 2.0 37.0 80-81 27.907641039524044 33.0 26.0 35.0 2.0 36.5 82-83 27.420973261517133 32.5 26.0 35.0 2.0 36.0 84-85 26.81383627687089 32.0 25.0 35.0 2.0 36.0 86-87 26.129206475812907 31.5 23.5 34.0 2.0 35.0 88-89 25.313397267193146 31.0 20.5 34.0 2.0 35.0 90-91 24.633733748920076 31.0 19.0 34.0 2.0 35.0 92-93 23.71245025086916 30.0 11.0 34.0 2.0 35.0 94-95 22.800223343050746 30.0 2.0 34.0 2.0 35.0 96-97 21.62613908589691 29.0 2.0 34.0 2.0 35.0 98-99 20.147304212585155 29.0 2.0 34.0 2.0 35.0 100-101 16.280792679326254 13.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2620124885501234 1101 2 0.29804761736250285 1101 3 0.3114370613564432 1101 4 0.17069801731362588 1101 5 0.07445209567308098 1101 6 0.16691929053947518 1101 7 0.19556129291295576 1101 8 0.1444674807308317 1101 9 0.29095030343906814 1101 10-11 0.2701835446616627 1101 12-13 0.3610454073921474 1101 14-15 0.38021810760601227 1101 16-17 0.36291652401460794 1101 18-19 0.4077225205577619 1101 20-21 0.37789949703795855 1101 22-23 0.35956637515979395 1101 24-25 0.3494821362005567 1101 26-27 0.37285530712366466 1101 28-29 0.4244661835201242 1101 30-31 0.5121225399344311 1101 32-33 0.38528145943944025 1101 34-35 0.35685533210676823 1101 36-37 0.4082510463166713 1101 38-39 0.4958073480162568 1101 40-41 0.47353158919040794 1101 42-43 0.5468866728018185 1101 44-45 0.5503228839015932 1101 46-47 0.49220419985643105 1101 48-49 0.7024842197267702 1101 50-51 0.6629497114192375 1101 52-53 0.606583181627478 1101 54-55 0.6432657510110644 1101 56-57 0.602395857897406 1101 58-59 1.0066866455617784 1101 60-61 0.8241071130379538 1101 62-63 1.0163205247083837 1101 64-65 0.8497862419065996 1101 66-67 0.7653753990308907 1101 68-69 0.8278051333276046 1101 70-71 0.8592047506725642 1101 72-73 0.8913378671103835 1101 74-75 0.9263745301294044 1101 76-77 0.6466728573041678 1101 78-79 0.9218893556640069 1101 80-81 0.8769886792408315 1101 82-83 1.011469567210014 1101 84-85 1.114712545602238 1101 86-87 1.193315898074271 1101 88-89 1.133755780735438 1101 90-91 1.1317008087415452 1101 92-93 1.434379061834914 1101 94-95 1.3356790283615858 1101 96-97 1.4323885246814108 1101 98-99 1.462692242719772 1101 100-101 1.5564339082998693 1102 1 0.36269004402746674 1102 2 0.34378655039503414 1102 3 0.3539107192315498 1102 4 0.27970468798493187 1102 5 0.19200739060911332 1102 6 0.2302529430833502 1102 7 0.23044768919584868 1102 8 0.2968486463136344 1102 9 0.4118583455135081 1102 10-11 0.48956949135515515 1102 12-13 0.4376892069760103 1102 14-15 0.5673408846880221 1102 16-17 0.6115298236525746 1102 18-19 0.5017008001455778 1102 20-21 0.3891707385306873 1102 22-23 0.4907261541334407 1102 24-25 0.4647701034968108 1102 26-27 0.5022349691004209 1102 28-29 0.5479054201343132 1102 30-31 0.4779192334935445 1102 32-33 0.5666227945935205 1102 34-35 0.6506676014823256 1102 36-37 0.718316210869709 1102 38-39 0.6136759460311012 1102 40-41 0.4440588172282034 1102 42-43 0.5511814019724 1102 44-45 0.6212473217904773 1102 46-47 0.6105665378208016 1102 48-49 0.6648358230964675 1102 50-51 0.8129179354602698 1102 52-53 0.7053659945025217 1102 54-55 0.769714272506576 1102 56-57 0.7972187603006518 1102 58-59 0.88762380627103 1102 60-61 0.7815119893314346 1102 62-63 1.0074232561971783 1102 64-65 1.0120900880620383 1102 66-67 0.9161649683909907 1102 68-69 0.8393557876885005 1102 70-71 0.7893406514536352 1102 72-73 1.0120032884413597 1102 74-75 1.0838606807445466 1102 76-77 1.0997491142164222 1102 78-79 1.3818085332118137 1102 80-81 1.37063079281263 1102 82-83 1.3913387199050007 1102 84-85 1.3017524108539291 1102 86-87 1.2564616516826952 1102 88-89 1.53294763912535 1102 90-91 1.6270949529277026 1102 92-93 1.8721830632427583 1102 94-95 1.9746217408469882 1102 96-97 1.7343429430735746 1102 98-99 2.2056340622447657 1102 100-101 2.145428254127598 1103 1 0.2973416522962822 1103 2 0.2545136634926486 1103 3 0.3099061431813226 1103 4 0.15937052989836076 1103 5 0.11420123422525563 1103 6 0.21461588771846607 1103 7 0.3084799875968969 1103 8 0.2359100239175902 1103 9 0.2919232615749081 1103 10-11 0.2738233557231595 1103 12-13 0.3682137234311753 1103 14-15 0.5509056810271886 1103 16-17 0.5110496044278463 1103 18-19 0.5861482154753475 1103 20-21 0.5039610915782831 1103 22-23 0.4999813487485909 1103 24-25 0.5439763450164818 1103 26-27 0.5834033251809885 1103 28-29 0.5450221094151857 1103 30-31 0.5611627751435861 1103 32-33 0.5015936621807242 1103 34-35 0.6594073253230093 1103 36-37 0.6619137312890047 1103 38-39 0.5346260085527135 1103 40-41 0.6254280406299202 1103 42-43 0.7559995571856319 1103 44-45 0.6706892831068885 1103 46-47 0.5836257072144804 1103 48-49 0.8775423439680452 1103 50-51 0.9604855785283561 1103 52-53 0.9855991868296528 1103 54-55 0.9697734685051955 1103 56-57 1.016007836310969 1103 58-59 1.0759768272623305 1103 60-61 0.983889141006216 1103 62-63 1.024669910625569 1103 64-65 1.018764603186952 1103 66-67 1.0422291661189256 1103 68-69 1.1579865642867908 1103 70-71 0.8918494975972493 1103 72-73 1.1581575439020249 1103 74-75 1.0270724294874647 1103 76-77 0.9679448999934195 1103 78-79 1.2348794087804933 1103 80-81 1.277796169139549 1103 82-83 1.433341273979547 1103 84-85 1.6048824824212282 1103 86-87 1.4905552212679218 1103 88-89 1.5357245300716862 1103 90-91 1.6863541382843472 1103 92-93 2.091012046667881 1103 94-95 1.7900100399929713 1103 96-97 2.1548440471787167 1103 98-99 2.3704915927739343 1103 100-101 2.2475646310664352 1104 1 0.2747132347320722 1104 2 0.2547710007425259 1104 3 0.2941779729119318 1104 4 0.25980737557132727 1104 5 0.07347055334855668 1104 6 0.1479137587234618 1104 7 0.11424214521282039 1104 8 0.17913734685701144 1104 9 0.26140761421481074 1104 10-11 0.26312777627499173 1104 12-13 0.3136217441938953 1104 14-15 0.41014102849523937 1104 16-17 0.4778852663494675 1104 18-19 0.42624998921941426 1104 20-21 0.3502444512729923 1104 22-23 0.41692104396086904 1104 24-25 0.3895069147988153 1104 26-27 0.37458944591609367 1104 28-29 0.36495115337591955 1104 30-31 0.33633004435601066 1104 32-33 0.47630125488489483 1104 34-35 0.6221495204725187 1104 36-37 0.5165236770598867 1104 38-39 0.5096136908855726 1104 40-41 0.6340268706869026 1104 42-43 0.8704146013723246 1104 44-45 0.5584313997999217 1104 46-47 0.4789608419085596 1104 48-49 0.6897253797944103 1104 50-51 0.8329530033984298 1104 52-53 0.7181026075321242 1104 54-55 0.6381508786882151 1104 56-57 0.719303479151975 1104 58-59 0.5801322746525486 1104 60-61 0.6204963847359721 1104 62-63 0.6832634056420162 1104 64-65 0.9261710516818482 1104 66-67 0.6784645261400186 1104 68-69 0.9465895054632618 1104 70-71 0.9532240394114133 1104 72-73 0.8052328432919502 1104 74-75 1.054852691720395 1104 76-77 0.7947596768004317 1104 78-79 1.0492921777293063 1104 80-81 1.1140476911952355 1104 82-83 1.2084198873711287 1104 84-85 1.3745711357314647 1104 86-87 1.3251058068670822 1104 88-89 1.1612902012825508 1104 90-91 1.3021260872688067 1104 92-93 1.8096388699964407 1104 94-95 1.9553170558851143 1104 96-97 2.022100000456316 1104 98-99 2.206879236820029 1104 100-101 2.1486204198992525 1105 1 0.17289523400118867 1105 2 0.2733591352492688 1105 3 0.2110639923462756 1105 4 0.15257401368157275 1105 5 0.06755252176475324 1105 6 0.18009327777738804 1105 7 0.1714866650755127 1105 8 0.21200431686078502 1105 9 0.21005117164617104 1105 10-11 0.17280801048758576 1105 12-13 0.3190624728919005 1105 14-15 0.4793411478630816 1105 16-17 0.3831954505084312 1105 18-19 0.35724260698470545 1105 20-21 0.36507444570149516 1105 22-23 0.3066473216610959 1105 24-25 0.29412618454318107 1105 26-27 0.20650459964151224 1105 28-29 0.25993871485709974 1105 30-31 0.4177551646224842 1105 32-33 0.40906183099574633 1105 34-35 0.5851528835904034 1105 36-37 0.49520801725674346 1105 38-39 0.4013378261052267 1105 40-41 0.4158046278295089 1105 42-43 0.5800752529679869 1105 44-45 0.6440768922783988 1105 46-47 0.5523935960257802 1105 48-49 0.7146776775879857 1105 50-51 0.7626794907786874 1105 52-53 0.8878294169209937 1105 54-55 0.7109204261088564 1105 56-57 0.4252787234292086 1105 58-59 0.7891871007379407 1105 60-61 0.6518612407157462 1105 62-63 0.734788902733456 1105 64-65 0.9481052675684012 1105 66-67 0.7236012412627417 1105 68-69 0.7845440992531394 1105 70-71 0.977373843061045 1105 72-73 1.0961109008760737 1105 74-75 1.0709442297833114 1105 76-77 0.9133021871795926 1105 78-79 1.0928502081748448 1105 80-81 0.9760414449769605 1105 82-83 1.2055766636305556 1105 84-85 1.1720634749218597 1105 86-87 1.3614245407292067 1105 88-89 1.4141959546029845 1105 90-91 1.386757065396182 1105 92-93 1.6842786696621523 1105 94-95 1.8791483518275065 1105 96-97 2.052461949372269 1105 98-99 2.0539725099851793 1105 100-101 1.8203720456683286 1106 1 0.09978857795378815 1106 2 0.095066364566744 1106 3 0.09504633611685165 1106 4 0.08968651983175135 1106 5 0.02931371528053006 1106 6 0.02842577071706387 1106 7 0.15213717976492802 1106 8 0.1472176506212577 1106 9 -0.13757440918374186 1106 10-11 0.07383550052647081 1106 12-13 0.08744769675295316 1106 14-15 0.187422000461126 1106 16-17 0.1865344459284657 1106 18-19 0.167655075155146 1106 20-21 0.280184069390927 1106 22-23 0.29043334107835506 1106 24-25 0.2729459496391158 1106 26-27 0.2558093814413027 1106 28-29 0.19025930945211655 1106 30-31 0.3892155441199421 1106 32-33 0.31829385316066094 1106 34-35 0.3373422741868026 1106 36-37 0.41148943919910863 1106 38-39 0.22309570125427314 1106 40-41 0.2518497198410401 1106 42-43 0.2853686404741538 1106 44-45 0.19192590733270976 1106 46-47 -0.48326005092778246 1106 48-49 -0.1714993806095393 1106 50-51 0.3180721422629631 1106 52-53 0.25224561862436445 1106 54-55 0.4608228842741937 1106 56-57 0.339326266069385 1106 58-59 0.06116530840140655 1106 60-61 0.41958473558551646 1106 62-63 0.24097991584822154 1106 64-65 0.25723955346310134 1106 66-67 0.1395442527583235 1106 68-69 -0.07987163950843978 1106 70-71 0.012425665690638965 1106 72-73 0.3684818683521911 1106 74-75 0.3068890856389821 1106 76-77 0.26303054219742705 1106 78-79 0.5430271167495846 1106 80-81 0.11208411117352668 1106 82-83 0.5663076635274145 1106 84-85 0.6965988341378377 1106 86-87 1.1115864314723503 1106 88-89 1.2294806073234987 1106 90-91 1.1785068686922138 1106 92-93 1.2789849858732438 1106 94-95 1.5173968430539269 1106 96-97 1.350324065679171 1106 98-99 1.5977049540752368 1106 100-101 1.600991987682093 1107 1 0.10648743450297005 1107 2 0.1483403816296196 1107 3 0.16357633209356948 1107 4 0.09207828717665478 1107 5 0.05224419074817632 1107 6 0.04736837729031862 1107 7 0.11826295146627075 1107 8 0.10371764575956632 1107 9 0.0868668222654776 1107 10-11 0.19930577717122588 1107 12-13 0.15485730282592414 1107 14-15 0.287677541822994 1107 16-17 0.29471600743313076 1107 18-19 0.26531034694097144 1107 20-21 0.3662360498318975 1107 22-23 0.333292428610946 1107 24-25 0.21626975956653638 1107 26-27 0.34603287418482154 1107 28-29 0.23965012152107334 1107 30-31 0.21577003236667025 1107 32-33 0.2851045725786676 1107 34-35 0.43450642369123926 1107 36-37 0.42028047299775295 1107 38-39 0.47181657263031695 1107 40-41 0.5113728046980484 1107 42-43 0.44709546457514193 1107 44-45 0.3669156014701187 1107 46-47 0.4838346644900682 1107 48-49 0.5397707107773613 1107 50-51 0.5582405695030204 1107 52-53 0.6142859390848585 1107 54-55 0.5113483043928895 1107 56-57 0.5207940227082943 1107 58-59 0.47205339948413183 1107 60-61 0.40250397229673496 1107 62-63 0.7055568269367498 1107 64-65 0.637833912326542 1107 66-67 0.706532176140314 1107 68-69 0.7819640313798253 1107 70-71 0.9566054080064639 1107 72-73 0.9977669194301946 1107 74-75 1.0385642345763522 1107 76-77 0.9233131695860521 1107 78-79 1.1869158548955454 1107 80-81 1.1498581475914271 1107 82-83 1.235042565920299 1107 84-85 1.220385884103706 1107 86-87 1.5681508210524875 1107 88-89 1.6484704731794189 1107 90-91 1.841008719344444 1107 92-93 1.952224472104664 1107 94-95 1.9775138428013044 1107 96-97 1.840102502934311 1107 98-99 2.0712348023667033 1107 100-101 2.025364568418688 1108 1 0.19765986105153033 1108 2 0.28449930486423725 1108 3 0.24062634668501204 1108 4 0.21186267715745544 1108 5 0.11717250994137629 1108 6 0.18596714474259812 1108 7 0.1427045865273726 1108 8 0.07724483761848688 1108 9 0.2698512623057212 1108 10-11 0.226967497145111 1108 12-13 0.24604855704208006 1108 14-15 0.3898179153749908 1108 16-17 0.45247026539322377 1108 18-19 0.33762243435893424 1108 20-21 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 15.0 4 46.0 5 89.0 6 160.0 7 469.0 8 1078.0 9 1805.0 10 2954.0 11 4641.0 12 6443.0 13 9133.0 14 12225.0 15 15890.0 16 19630.0 17 24399.0 18 23614.0 19 21205.0 20 21489.0 21 23320.0 22 25422.0 23 28815.0 24 32974.0 25 38220.0 26 45898.0 27 54756.0 28 64390.0 29 76429.0 30 89937.0 31 106100.0 32 126309.0 33 154251.0 34 191474.0 35 242740.0 36 301483.0 37 308229.0 38 122332.0 39 3181.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.937337414724354 19.66725973717453 15.240034575844058 23.155368272257064 2 15.95136912934072 20.847040987305565 39.11892903741252 24.082660845941195 3 19.28903597744494 24.489018171775655 29.951861101244308 26.2700847495351 4 13.412859338718643 17.3094882500645 36.17847292627356 33.09917948494329 5 14.05446868920601 37.49006506050486 33.78374755233538 14.671718697953754 6 32.57386634107699 36.08172264556148 17.01442260522615 14.32998840813538 7 28.973321474999842 32.49371123746676 20.14634261559831 18.38662467193509 8 26.626198135310368 35.37568599520519 19.345632569112798 18.652483300371646 9 26.439738256661453 16.67060329423051 19.74412526470035 37.14553318440769 10-11 25.265654226620747 26.71897384837746 26.975225591470718 21.040146333531073 12-13 25.96127902846454 23.864843160215592 27.58545585692963 22.588421954390235 14-15 22.69128149037086 25.293021358627072 25.791148583767953 26.22454856723412 16-17 22.512293633655624 28.67471313340716 26.328475534919555 22.484517698017665 18-19 22.432713193365025 26.987171742039457 28.80305476242604 21.777060302169478 20-21 23.165334723871318 26.20342704626658 28.749615043246884 21.881623186615222 22-23 22.808017638514023 26.190708711060317 28.58439024212985 22.416883408295806 24-25 22.658973401544287 26.31886167294902 28.749240258271303 22.272924667235394 26-27 22.32289368749561 26.767258892875322 28.50253384574259 22.407313573886476 28-29 22.273913217269335 26.826854244112315 28.71675515002198 22.182477388596368 30-31 22.40287820109252 26.488702774545153 28.60815329813937 22.500265726222953 32-33 22.219898089426575 26.571015353811745 28.479204083690586 22.729882473071097 34-35 22.849952243748813 26.66232071531028 28.26922487231875 22.218502168622155 36-37 22.80148747526743 26.294510107698844 28.300716250690012 22.603286166343715 38-39 22.45161515981482 26.635901934121716 28.449829509205003 22.46265339685846 40-41 22.72793239412053 26.40245429928216 28.446515130765064 22.423098175832248 42-43 22.610208239695613 26.380889069874502 28.242075778817917 22.76682691161197 44-45 22.56663117364989 26.63114185153622 28.207158989939003 22.595067984874888 46-47 22.68219696522353 26.747862638345964 27.763398993252924 22.806541403177587 48-49 22.699162315936164 26.858148773634525 27.712525652427882 22.730163258001422 50-51 22.6772456490585 26.965390057391776 27.565471364298094 22.791892929251638 52-53 23.089365382326783 26.867051608278906 27.388298949919736 22.65528405947457 54-55 22.5909078933861 26.766329898424445 27.684334507979507 22.958427700209945 56-57 22.639953923288452 27.131320444814687 27.487184006148407 22.74154162574845 58-59 22.71015590055748 26.698115286314422 27.545551544592 23.04617726853609 60-61 22.578088307035145 26.839230250015216 27.724857895315385 22.857823547634254 62-63 22.61184907172323 26.89374684566697 27.658736486053705 22.835667596556092 64-65 22.33726238090652 26.703538777727907 27.608176768071406 23.351022073294168 66-67 22.334635228576648 27.014780553882385 27.4633347960349 23.187249421506074 68-69 22.51244560866954 26.69536493641132 27.605400381068684 23.186789073850456 70-71 22.28334348151649 26.822136378409056 27.42809760515747 23.46642253491698 72-73 22.52035901175698 26.795586823517226 27.295963036842107 23.388091127883687 74-75 22.41003705058279 26.88148129638422 27.201261219102747 23.507220433930247 76-77 22.344216886360073 26.80512539680015 27.458320362295296 23.392337354544484 78-79 22.432559287078362 26.927372448666638 27.306497144978394 23.333571119276613 80-81 22.310144051759583 27.09779208476359 27.223783420303548 23.368280443173273 82-83 22.510820150357493 26.968426744458153 27.126713332469993 23.394039772714365 84-85 22.442930921064587 26.71143634903271 27.171482542186233 23.67415018771647 86-87 22.489730605124876 26.83157510996231 27.15192798874313 23.526766296169686 88-89 22.319812662723347 26.816352823993068 27.019497024325023 23.844337488958566 90-91 22.257085337545984 26.83584515869911 27.203384478523063 23.703685025231838 92-93 22.417678477640205 26.5915691202742 27.141636271447027 23.849116130638564 94-95 22.177761946345377 26.52794273060102 27.125021467846388 24.169273855207212 96-97 22.378659073967246 26.36691650264767 27.138780861908007 24.115643561477075 98-99 22.124786456077555 26.429045700103664 26.900762463716283 24.5454053801025 100-101 22.161377839076447 26.249361724552116 27.304515083289267 24.284745353082165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 514.0 1 363.5 2 265.5 3 350.5 4 330.0 5 333.5 6 538.5 7 1131.0 8 1785.0 9 2004.5 10 1762.5 11 1464.5 12 1473.5 13 1624.0 14 1795.0 15 1936.0 16 2110.5 17 2293.0 18 2451.5 19 2665.0 20 3087.0 21 3588.5 22 4170.0 23 5027.5 24 6139.0 25 7684.5 26 9893.0 27 12562.0 28 15142.5 29 18231.0 30 21912.5 31 25848.5 32 30778.0 33 34752.5 34 37984.0 35 42121.0 36 46941.0 37 51936.5 38 56478.5 39 59544.5 40 62531.0 41 67529.5 42 72371.0 43 77193.5 44 80724.5 45 83287.0 46 84608.0 47 86311.5 48 90224.0 49 91294.0 50 89524.5 51 86356.0 52 82176.0 53 80116.5 54 78537.5 55 72467.5 56 64582.5 57 57817.5 58 51709.0 59 45181.5 60 37764.5 61 30722.0 62 25023.0 63 19553.0 64 14796.0 65 11551.5 66 8803.5 67 6894.0 68 4997.0 69 3364.0 70 2628.0 71 2162.5 72 1850.0 73 1602.5 74 1309.5 75 1093.5 76 802.0 77 477.5 78 315.5 79 216.5 80 129.0 81 76.5 82 47.0 83 28.5 84 16.5 85 9.0 86 6.5 87 3.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.088036316297729E-4 2 0.05146383495961475 3 0.0 4 9.084525147328286E-5 5 0.04446875059617196 6 9.084525147328286E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006359167603129801 26-27 0.0022938425997003926 28-29 9.084525147328286E-5 30-31 0.0014535240235725258 32-33 3.6338100589313144E-4 34-35 0.011968861881605017 36-37 0.022756735494057358 38-39 0.004882932266688954 40-41 9.765864533377907E-4 42-43 9.311638276011494E-4 44-45 0.007903536878175608 46-47 0.0 48-49 0.0 50-51 4.996488831030557E-4 52-53 0.0 54-55 0.0014535240235725258 56-57 0.0 58-59 0.02250691105250583 60-61 0.0 62-63 0.00949332877895806 64-65 0.01517115699603824 66-67 0.006359167603129801 68-69 0.016715526271084045 70-71 1.1355656434160358E-4 72-73 1.1355656434160358E-4 74-75 0.022756735494057358 76-77 0.051372989708141456 78-79 0.031205343881072666 80-81 0.0272989980677215 82-83 0.035929296957683377 84-85 0.01607960951077107 86-87 0.010151956852139361 88-89 0.08525826850767597 90-91 0.025300402535309276 92-93 0.024619063149259657 94-95 0.02634512292725203 96-97 0.03795060380296392 98-99 0.05355327574350025 100-101 0.013717632972465714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2201546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.994665529580864 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97600642218491 38.46357711135631 2 14.226716535166844 14.794267356603152 3 5.019941511144785 7.830305395500956 4 2.3030677752737714 4.789889546692627 5 1.22826973004992 3.193173689702708 6 0.7279344633495167 2.2709225369587838 7 0.46452655300592344 1.6907031927206475 8 0.3431651772909557 1.427420689171405 9 0.237147459986589 1.1097362576873122 >10 1.3006970419732704 12.319908423130007 >50 0.10537179490719645 3.7886920107117015 >100 0.06199303484636197 5.753510338735761 >500 0.004112500653180225 1.3860155198268982 >1k 8.750001389744829E-4 0.6387545674829828 >5k 1.7500002779489656E-4 0.5431233637187927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6529 0.29656432343453193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.2456909826094935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.1736506981911802 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.542262573664143E-5 0.0 16-17 0.0 0.0 0.0 9.084525147328286E-5 0.0 18-19 0.0 0.0 0.0 1.362678772099243E-4 0.0 20-21 0.0 0.0 0.0 1.58979190078245E-4 0.0 22-23 0.0 0.0 0.0 5.677828217080179E-4 0.0 24-25 0.0 0.0 0.0 0.0011809882691526771 0.0 26-27 0.0 0.0 0.0 0.0019758842195439024 0.0 28-29 0.0 0.0 0.0 0.00474666438947903 0.0 30-31 0.0 0.0 0.0 0.015080311744564954 0.0 32-33 0.0 0.0 0.0 0.034680174749925734 0.0 34-35 0.0 0.0 0.0 0.06434114935595259 0.0 36-37 0.0 0.0 0.0 0.11403350191183832 0.0 38-39 0.0 0.0 0.0 0.1856195600727852 0.0 40-41 0.0 0.0 0.0 0.26731215246013484 0.0 42-43 0.0 0.0 0.0 0.3496179502949291 0.0 44-45 0.0 0.0 0.0 0.45116023012919104 0.0 46-47 0.0 0.0 0.0 0.5736196291151763 0.0 48-49 0.0 0.0 0.0 0.7088654972460262 0.0 50-51 0.0 0.0 0.0 0.851469830746212 0.0 52-53 0.0 0.0 0.0 0.9945056791908958 0.0 54-55 0.0 0.0 0.0 1.1535075805820092 0.0 56-57 0.0 0.0 0.0 1.3379234410727734 0.0 58-59 0.0 0.0 0.0 1.5413032478085853 0.0 60-61 0.0 0.0 0.0 1.7523367669810215 0.0 62-63 0.0 0.0 0.0 1.9630977503990377 0.0 64-65 0.0 0.0 0.0 2.1915962691672126 0.0 66-67 0.0 0.0 0.0 2.4497103399156774 0.0 68-69 0.0 0.0 0.0 2.7382121472819554 0.0 70-71 0.0 0.0 0.0 3.049016463884924 0.0 72-73 0.0 0.0 0.0 3.3630003642894586 0.0 74-75 0.0 0.0 0.0 3.6858189653997693 0.0 76-77 0.0 0.0 0.0 4.034778287621517 0.0 78-79 0.0 0.0 0.0 4.41585140623907 0.0 80-81 0.0 0.0 0.0 4.827380395413042 0.0 82-83 0.0 0.0 0.0 5.251673142419009 0.0 84-85 0.0 0.0 0.0 5.684346363873387 0.0 86-87 0.0 0.0 0.0 6.121130332956931 0.0 88-89 0.0 0.0 0.0 6.602224073446569 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3685 0.0 57.009533 1 GTATCAA 6815 0.0 42.403378 1 ATCAACG 8915 0.0 32.238853 3 TATCAAC 9115 0.0 31.547243 2 TCAACGC 9225 0.0 31.206985 4 CAACGCA 9415 0.0 30.526752 5 AACGCAG 9700 0.0 29.712929 6 TGGTATC 1595 0.0 27.4151 2 GTGGTAT 1795 0.0 25.949223 1 ACGCAGA 11435 0.0 25.080101 7 CGCAGAG 11545 0.0 24.841139 8 GTACATG 9420 0.0 23.916086 1 TACATGG 9390 0.0 23.536943 2 ACATGGG 9585 0.0 22.650074 3 GCAGAGT 12615 0.0 22.357729 9 GAGTACT 6705 0.0 20.926046 12-13 GTCTACG 160 4.968179E-5 20.79413 1 AGTACTT 7140 0.0 20.282904 12-13 AGAGTAC 12085 0.0 20.155762 10-11 CGCGGAA 295 0.0 20.139593 82-83 >>END_MODULE