FastQCFastQC Report
Thu 26 May 2016
SRR938370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235453
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68450.30620191970039184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.23690947651326152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.16824330460090192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.058.9762761
GTATCAA69750.044.5084461
ATCAACG93800.032.526273
TCAACGC95350.031.947694
TATCAAC95450.031.914222
CAACGCA97350.031.2418255
AACGCAG101000.030.1530936
GTGGTAT17300.026.1466791
ACGCAGA116750.025.960347
GTACATG100000.025.7118191
CGCAGAG118850.025.4216988
TGGTATC16550.024.981782
TACATGG102450.024.7239232
ACATGGG101650.023.9367223
GCAGAGT128050.023.0006079
TAAGGTG9100.022.455335
GTAAGGT8200.022.0227664
GAGTACT67650.021.27420812-13
AGAGTAC121550.020.84862710-11
AGTACTT73050.020.57936912-13
Produced by FastQC (version 0.11.5)