##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235453 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.204013235796054 31.0 31.0 34.0 28.0 34.0 2 31.302915784854346 31.0 31.0 34.0 28.0 34.0 3 31.37055576654933 31.0 31.0 34.0 28.0 34.0 4 35.01491330839879 37.0 35.0 37.0 32.0 37.0 5 35.03376765246239 37.0 35.0 37.0 32.0 37.0 6 35.07446902260973 37.0 35.0 37.0 32.0 37.0 7 35.0025305832867 37.0 35.0 37.0 32.0 37.0 8 34.966907378504494 37.0 35.0 37.0 32.0 37.0 9 36.489962884480235 39.0 37.0 39.0 32.0 39.0 10-11 36.3444818566975 39.0 35.0 39.0 32.0 39.0 12-13 36.2003450754724 39.0 35.0 39.0 32.0 39.0 14-15 37.210408583853024 39.0 36.5 41.0 31.5 41.0 16-17 37.06509776765604 39.0 36.0 41.0 31.0 41.0 18-19 37.05706225986411 39.0 36.0 41.0 31.0 41.0 20-21 37.15644301177434 39.0 36.0 41.0 31.0 41.0 22-23 37.08129381382655 39.0 36.0 41.0 31.0 41.0 24-25 36.96838649705451 39.0 36.0 41.0 30.5 41.0 26-27 36.796874727404244 39.0 36.0 41.0 30.0 41.0 28-29 36.618518483725666 39.0 36.0 41.0 30.0 41.0 30-31 36.40853084363661 39.0 35.5 40.0 30.0 41.0 32-33 36.1495130964507 38.5 35.0 40.0 29.5 41.0 34-35 35.90558177693738 38.0 35.0 40.0 28.5 41.0 36-37 35.620983979533456 38.0 35.0 40.0 27.0 41.0 38-39 35.38933048469371 38.0 34.0 40.0 26.5 41.0 40-41 35.32893288295482 38.0 34.0 40.0 26.5 41.0 42-43 35.1558046624107 38.0 34.0 40.0 26.0 41.0 44-45 34.8771924527154 38.0 34.0 40.0 24.5 41.0 46-47 34.51695897878417 38.0 33.0 40.0 24.0 41.0 48-49 34.167908696805526 38.0 33.0 40.0 23.0 41.0 50-51 33.43404960873702 37.5 32.0 39.5 22.0 40.5 52-53 33.51832581584135 37.5 33.0 39.5 22.0 40.5 54-55 33.6500264152277 38.0 33.0 40.0 21.5 41.0 56-57 33.36299600125791 37.0 32.5 40.0 19.5 41.0 58-59 33.04488262557969 37.0 32.0 40.0 18.5 41.0 60-61 32.60678752807597 36.5 31.0 39.5 16.5 41.0 62-63 32.0918710883208 36.0 31.0 39.0 13.0 40.5 64-65 31.60344793650325 35.0 30.5 39.0 8.0 40.0 66-67 31.06873371974271 35.0 29.5 38.5 7.5 40.0 68-69 30.514457025041455 35.0 29.0 38.0 4.0 40.0 70-71 29.894217860988356 34.0 28.5 37.0 2.0 39.5 72-73 29.338632706659453 34.0 28.0 36.5 2.0 39.0 74-75 28.719925223209792 34.0 26.5 36.0 2.0 39.0 76-77 27.96811898975286 33.0 26.0 35.5 2.0 38.0 78-79 27.176394225242042 32.5 25.0 35.0 2.0 37.0 80-81 26.50716476705169 32.0 23.5 35.0 2.0 36.5 82-83 25.8390603604728 31.5 21.0 35.0 2.0 36.0 84-85 25.157791508029916 31.0 19.5 34.0 2.0 35.5 86-87 24.467647720618594 31.0 16.5 34.0 2.0 35.0 88-89 23.669482874388322 30.5 7.5 34.0 2.0 35.0 90-91 22.814527748961844 30.0 2.0 34.0 2.0 35.0 92-93 21.935407051725086 29.0 2.0 34.0 2.0 35.0 94-95 20.946983005234287 29.0 2.0 34.0 2.0 35.0 96-97 19.667595113831517 26.5 2.0 33.5 2.0 35.0 98-99 18.149091034345165 24.5 2.0 33.0 2.0 34.5 100-101 14.649769196668416 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5310257364289832 1101 2 0.4963006458471959 1101 3 0.48876342027213227 1101 4 0.41731778841297285 1101 5 0.49604950808255666 1101 6 0.3936315775783825 1101 7 0.3551381676362837 1101 8 0.46459495262232053 1101 9 0.4309384423997784 1101 10-11 0.5516722246497778 1101 12-13 0.5173827397311612 1101 14-15 0.6803863020057506 1101 16-17 0.7000438529471467 1101 18-19 0.607566127229596 1101 20-21 0.7179737379960969 1101 22-23 0.7520668547206313 1101 24-25 0.7939718241668885 1101 26-27 0.7467487462003604 1101 28-29 0.8807212659771722 1101 30-31 0.9038194520065161 1101 32-33 0.8831251392630008 1101 34-35 0.7809566957219047 1101 36-37 0.9810372501718945 1101 38-39 0.9828443124873303 1101 40-41 0.9106374791803447 1101 42-43 0.9569652254792587 1101 44-45 0.9212091144860679 1101 46-47 0.9635231926717438 1101 48-49 1.0058036748354624 1101 50-51 0.9904265620626305 1101 52-53 1.1107998872775653 1101 54-55 1.2386497314199545 1101 56-57 1.2150160247720905 1101 58-59 1.1331477177612825 1101 60-61 1.082651723085661 1101 62-63 1.0574944304404923 1101 64-65 0.8427541595876136 1101 66-67 0.8396840026025529 1101 68-69 1.0138913819956485 1101 70-71 1.1730887056563368 1101 72-73 1.1271741826344304 1101 74-75 1.1926771857233724 1101 76-77 1.2853785589566336 1101 78-79 1.0676905835819461 1101 80-81 1.017506665404298 1101 82-83 1.121536526060254 1101 84-85 1.3263995238529809 1101 86-87 1.4067148159074385 1101 88-89 1.3554771127942438 1101 90-91 1.1698876252689345 1101 92-93 1.1786483214577288 1101 94-95 1.022596041346496 1101 96-97 1.2903744409638271 1101 98-99 1.2991145963747535 1101 100-101 1.2493688907187703 1102 1 0.5605639928388229 1102 2 0.4951877763175716 1102 3 0.5374303881889873 1102 4 0.5073670083708706 1102 5 0.5764411603452757 1102 6 0.4839466542046793 1102 7 0.43140600201892454 1102 8 0.41777986282477286 1102 9 0.4818378363195208 1102 10-11 0.5992011963396848 1102 12-13 0.6559102747413661 1102 14-15 0.5525045839070302 1102 16-17 0.5897481887208826 1102 18-19 0.6435905163367721 1102 20-21 0.5105903390429063 1102 22-23 0.646490652945154 1102 24-25 0.49591985243037584 1102 26-27 0.4718783817214032 1102 28-29 0.38866697610018974 1102 30-31 0.22008311211525466 1102 32-33 0.2627060502678944 1102 34-35 0.26579800239121454 1102 36-37 0.2657274927053166 1102 38-39 0.35580747958594827 1102 40-41 0.36531712392126536 1102 42-43 0.3756104506975433 1102 44-45 0.4932090577483663 1102 46-47 0.40670656285080753 1102 48-49 0.042695340269432336 1102 50-51 0.23309169410950403 1102 52-53 0.18736670909483166 1102 54-55 0.23466056426865123 1102 56-57 0.15876586013143168 1102 58-59 0.5292304859127981 1102 60-61 0.5025013073806619 1102 62-63 0.27448747399275675 1102 64-65 0.2985963193488246 1102 66-67 0.31094676098706486 1102 68-69 0.11475693592787195 1102 70-71 0.22162801374004104 1102 72-73 0.18544339898542717 1102 74-75 0.23096135651196548 1102 76-77 0.22142422470266254 1102 78-79 0.1695243258995589 1102 80-81 0.42341518801528366 1102 82-83 0.43707191721606264 1102 84-85 0.6434530538499992 1102 86-87 0.5620855461419438 1102 88-89 0.5558098388990587 1102 90-91 0.5973118956321954 1102 92-93 0.5848442813279604 1102 94-95 0.6375679055245378 1102 96-97 0.7761070361666533 1102 98-99 0.7750299477736426 1102 100-101 0.8551369875132924 1103 1 0.3931523084045274 1103 2 0.470629797843813 1103 3 0.3524699660253994 1103 4 0.46841705352943563 1103 5 0.4721650975413425 1103 6 0.3297524289797735 1103 7 0.3457924568150048 1103 8 0.2321655288249076 1103 9 0.289075039091351 1103 10-11 0.39320220599849875 1103 12-13 0.39233489937309685 1103 14-15 0.4962782231354339 1103 16-17 0.5694364960042293 1103 18-19 0.5743362045809448 1103 20-21 0.45218492948033173 1103 22-23 0.36398338616253056 1103 24-25 0.3766840773791955 1103 26-27 0.38410412723217036 1103 28-29 0.4549453579641991 1103 30-31 0.6775750959764864 1103 32-33 0.5987698007559601 1103 34-35 0.6824250320617793 1103 36-37 0.6443647293953703 1103 38-39 0.4154372976501648 1103 40-41 0.5419963718832079 1103 42-43 0.4476866799374264 1103 44-45 0.48566284416608596 1103 46-47 0.4990846248580638 1103 48-49 0.5363632808415417 1103 50-51 0.4102552627801046 1103 52-53 0.4054319109674367 1103 54-55 0.4681327256878589 1103 56-57 0.47377980209972037 1103 58-59 0.47482703256415704 1103 60-61 0.4071806421101698 1103 62-63 0.5167024524389134 1103 64-65 0.42027262904588625 1103 66-67 0.4938920067167203 1103 68-69 0.2847988604322147 1103 70-71 0.5066755978552671 1103 72-73 0.5896492589504803 1103 74-75 0.5900023384051529 1103 76-77 0.6220175964503589 1103 78-79 0.4776560232701037 1103 80-81 0.38202982570158994 1103 82-83 0.6467778540887217 1103 84-85 0.640908595338697 1103 86-87 0.6147980181889885 1103 88-89 0.396756039071807 1103 90-91 0.4766811349568272 1103 92-93 0.383465860418557 1103 94-95 0.4349459478118902 1103 96-97 0.6652439356464406 1103 98-99 0.5868626757060866 1103 100-101 0.6900832113499185 1104 1 0.5773605558955204 1104 2 0.49906431971704635 1104 3 0.5351187443533725 1104 4 0.5453043048878499 1104 5 0.3756986393059023 1104 6 0.4608536355917394 1104 7 0.47549232488888293 1104 8 0.3466479597594798 1104 9 0.557605312822993 1104 10-11 0.5599750460847659 1104 12-13 0.5206240163716842 1104 14-15 0.5555203557603363 1104 16-17 0.6145281967066296 1104 18-19 0.6746523905305111 1104 20-21 0.7793357318803018 1104 22-23 0.7669113252837434 1104 24-25 0.7074696251436876 1104 26-27 0.687958147063263 1104 28-29 0.7394901224851012 1104 30-31 0.6642057163906969 1104 32-33 0.8168793717249798 1104 34-35 0.8052345464049822 1104 36-37 0.8013481542938621 1104 38-39 0.753859801595901 1104 40-41 0.6373971426570577 1104 42-43 0.4949436406969312 1104 44-45 0.5955988313831071 1104 46-47 0.5888572631395235 1104 48-49 0.6506469189904891 1104 50-51 0.623563122022766 1104 52-53 0.6873891690125191 1104 54-55 0.8777087398322649 1104 56-57 0.8399440651808803 1104 58-59 0.7963865996175699 1104 60-61 0.9490156049419411 1104 62-63 1.0364685923046935 1104 64-65 1.078273549215524 1104 66-67 1.1641617926229202 1104 68-69 1.1545645484962108 1104 70-71 1.1757162946541015 1104 72-73 1.2326627843899338 1104 74-75 1.2008854151150743 1104 76-77 1.1371857118947517 1104 78-79 1.3084106979125814 1104 80-81 1.2796603888875744 1104 82-83 1.097090576224602 1104 84-85 1.144656291149591 1104 86-87 1.252193200174748 1104 88-89 1.065986869122046 1104 90-91 1.0362681429446852 1104 92-93 1.0422787060469219 1104 94-95 0.9863886638635044 1104 96-97 1.0242985328802092 1104 98-99 0.9167193942998395 1104 100-101 0.8801387151992692 1105 1 0.29934053192581445 1105 2 0.3266745716884678 1105 3 0.33142869757191207 1105 4 0.28388069819949635 1105 5 0.23757971134239853 1105 6 0.3512070585123368 1105 7 0.3321861118711311 1105 8 0.2703519888705159 1105 9 0.264645473663208 1105 10-11 0.35721172668551304 1105 12-13 0.34969365589969925 1105 14-15 0.371600975614669 1105 16-17 0.47344453721543545 1105 18-19 0.38715593677352445 1105 20-21 0.4030181940440585 1105 22-23 0.49301597998099567 1105 24-25 0.44595700051857534 1105 26-27 0.6236937102355924 1105 28-29 0.5626614190494408 1105 30-31 0.5441033312889729 1105 32-33 0.3925033657499313 1105 34-35 0.45520226835636635 1105 36-37 0.5094536354828492 1105 38-39 0.4576417612063608 1105 40-41 0.5805108815261946 1105 42-43 0.544754604232871 1105 44-45 0.5330175934692818 1105 46-47 0.5113635305812565 1105 48-49 0.4717443871177238 1105 50-51 0.2857728005160354 1105 52-53 0.33890475366496275 1105 54-55 0.46178473364876993 1105 56-57 0.4191830559056484 1105 58-59 0.6058362878376684 1105 60-61 0.6181044520800469 1105 62-63 0.6386653451040587 1105 64-65 0.6344921758207391 1105 66-67 0.7599052878000272 1105 68-69 0.5603291112516864 1105 70-71 0.6207573080400799 1105 72-73 0.5470656332533963 1105 74-75 0.47305051950488775 1105 76-77 0.5774058250271388 1105 78-79 0.7108872058619546 1105 80-81 0.676671521195729 1105 82-83 0.6922445989668731 1105 84-85 0.5657335674033668 1105 86-87 0.6498638251240862 1105 88-89 0.775670609380736 1105 90-91 0.6098306118245951 1105 92-93 0.7605810833324398 1105 94-95 0.7673066559083388 1105 96-97 0.7855786614713232 1105 98-99 0.8391066092976978 1105 100-101 0.5804194835615704 1106 1 0.27269006609088464 1106 2 0.23769001458584427 1106 3 -0.03861985000936485 1106 4 0.26027850501006355 1106 5 0.29757410279896135 1106 6 0.08791358415513173 1106 7 0.20379732705432474 1106 8 0.24468375032290623 1106 9 0.16090920260508312 1106 10-11 0.07754291630077148 1106 12-13 0.06470331892215597 1106 14-15 0.2980227065971519 1106 16-17 0.1373837811676708 1106 18-19 -0.21337491625591554 1106 20-21 0.2280713598874442 1106 22-23 0.16016933666862343 1106 24-25 0.36609456359180115 1106 26-27 0.18780426736677924 1106 28-29 0.23119174270853193 1106 30-31 0.18306497097520236 1106 32-33 0.4131402063387952 1106 34-35 0.4178844956802976 1106 36-37 0.12701302582100737 1106 38-39 0.24087439458224935 1106 40-41 0.456056131159464 1106 42-43 0.0556983112238143 1106 44-45 0.3908610609256371 1106 46-47 0.12918431125974195 1106 48-49 0.503161016595449 1106 50-51 0.12039755711001021 1106 52-53 0.5137480750502448 1106 54-55 0.0557592286876627 1106 56-57 -0.33044636654251036 1106 58-59 -0.7200890028492566 1106 60-61 -0.05555684681028339 1106 62-63 0.04187449728046744 1106 64-65 0.16689981881571825 1106 66-67 -0.22283526178597768 1106 68-69 -0.622561939131117 1106 70-71 -0.6755268122770879 1106 72-73 -0.06635818848570807 1106 74-75 -0.20277347327283124 1106 76-77 -0.19272104813301283 1106 78-79 -0.17949899287451032 1106 80-81 -0.19586819713970272 1106 82-83 -0.21853132764174887 1106 84-85 -0.1712995131184023 1106 86-87 0.03374372907595102 1106 88-89 -0.06445945244869833 1106 90-91 0.07678615020632407 1106 92-93 0.0845900606628689 1106 94-95 -0.3383782362059087 1106 96-97 -0.2371869178871826 1106 98-99 -0.422640214022568 1106 100-101 -0.720956073952177 1107 1 0.3345998857398129 1107 2 0.43483630576463383 1107 3 0.3471874898769265 1107 4 0.42869764139817335 1107 5 0.3058974499106384 1107 6 0.3596540537693542 1107 7 0.3500934982112369 1107 8 0.3073311923316524 1107 9 0.23502678820324263 1107 10-11 0.3377075111050303 1107 12-13 0.3403416689374268 1107 14-15 0.4088859030569836 1107 16-17 0.3418060028391494 1107 18-19 0.43244510781086376 1107 20-21 0.3526833592933585 1107 22-23 0.5309653394222593 1107 24-25 0.4663455013992035 1107 26-27 0.35508291513399826 1107 28-29 0.4578236720037623 1107 30-31 0.4513185390472998 1107 32-33 0.5021291644690535 1107 34-35 0.5736179360404847 1107 36-37 0.531266006220072 1107 38-39 0.6225749769597968 1107 40-41 0.42307521145474425 1107 42-43 0.6835782611788943 1107 44-45 0.6519972061864223 1107 46-47 0.5271917502445405 1107 48-49 0.623813444196756 1107 50-51 0.553922573598193 1107 52-53 0.4858684638629853 1107 54-55 0.5429264361050414 1107 56-57 0.5853068899523421 1107 58-59 0.4446326610499156 1107 60-61 0.5740460941223304 1107 62-63 0.7261237191396859 1107 64-65 0.4387886330766335 1107 66-67 0.49414954055458793 1107 68-69 0.42515769875817355 1107 70-71 0.4700134608874542 1107 72-73 0.523345837199308 1107 74-75 0.5432988258427542 1107 76-77 0.5947766718703882 1107 78-79 0.6245713381783169 1107 80-81 0.6941592983266034 1107 82-83 0.7933716364162322 1107 84-85 0.9190627919363159 1107 86-87 0.9469873400485334 1107 88-89 0.9841615752979926 1107 90-91 0.9153341306954346 1107 92-93 0.9987260574007131 1107 94-95 1.1106048451941142 1107 96-97 0.8610565531207968 1107 98-99 0.5150690308265133 1107 100-101 0.1561903099420192 1108 1 0.30953147220725086 1108 2 0.3839317941040612 1108 3 0.30129593440747726 1108 4 0.307807902217192 1108 5 0.4016267502763142 1108 6 0.39756725939742665 1108 7 0.3878347618636724 1108 8 0.2739768707759396 1108 9 0.20227910699819773 1108 10-11 0.24559867909960786 1108 12-13 0.3872551893888385 1108 14-15 0.5433390791400967 1108 16-17 0.2215537809129131 1108 18-19 0.4465637235568778 1108 20-21 0.49041190892646824 1108 22-23 0.49076845375157063 1108 24-25 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-0.9354674162830037 2316 54-55 -0.9510798941495153 2316 56-57 -0.7611709251761241 2316 58-59 -0.8890225027779834 2316 60-61 -1.049296677625641 2316 62-63 -0.9776446516164974 2316 64-65 -0.9365474159208702 2316 66-67 -0.9247200078570614 2316 68-69 -0.8605319860728287 2316 70-71 -1.1770423259019047 2316 72-73 -1.1477642087018403 2316 74-75 -0.8150450948782932 2316 76-77 -0.8894284428396162 2316 78-79 -0.9059162382824368 2316 80-81 -0.854905765604947 2316 82-83 -0.759353833940537 2316 84-85 -0.8939455214849019 2316 86-87 -1.1465673962644765 2316 88-89 -1.338980531517926 2316 90-91 -1.1557221660031445 2316 92-93 -1.123624944647311 2316 94-95 -1.2056560031897092 2316 96-97 -1.1116902342740822 2316 98-99 -1.2039121906856174 2316 100-101 -0.8121695063042935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6721.0 3 2205.0 4 2245.0 5 2437.0 6 2902.0 7 3679.0 8 4517.0 9 5956.0 10 7736.0 11 9558.0 12 11832.0 13 14271.0 14 16512.0 15 19602.0 16 22911.0 17 26405.0 18 25615.0 19 23126.0 20 25217.0 21 27703.0 22 30715.0 23 34395.0 24 39035.0 25 43858.0 26 50163.0 27 57373.0 28 65932.0 29 76236.0 30 89719.0 31 105148.0 32 125600.0 33 151106.0 34 185098.0 35 227590.0 36 274224.0 37 277803.0 38 133255.0 39 7053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48621624867576 18.702038299451303 14.48320119311592 23.328544258757017 2 15.46604176885703 19.97093616518498 40.44652863060734 24.116493435350645 3 19.230822591884312 24.245965271415805 29.49235436779145 27.030857768908433 4 12.905501210237608 16.402523954111228 36.66827494593317 34.023699889717996 5 14.133081003512357 37.239208580765606 33.72622602348225 14.901484392239784 6 32.57250108661545 35.560995720234686 17.476357441200786 14.390145751949083 7 29.204071669036153 32.34231053390201 20.28061527005662 18.173002527005213 8 26.624310818774816 34.97888099477473 19.600960658553184 18.79584752789727 9 26.772769155931186 16.823646976217784 19.878688015451747 36.524895852399275 10-11 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28.20860021737861 22.290795468302758 36-37 22.818215241786007 26.29643661048687 28.187321582695336 22.698026565031785 38-39 22.467790797856622 26.63324655020859 28.317877256579376 22.581085395355412 40-41 22.745658571376794 26.353727939376824 28.424248552857122 22.476364936389253 42-43 22.630459196685443 26.326404144984988 28.21990254989955 22.823234108430015 44-45 22.533662854536697 26.642768998076154 28.16294613249875 22.6606220148884 46-47 22.68665316904441 26.74584943641723 27.65777778672549 22.909719607812875 48-49 22.698801886077064 26.834365184837388 27.66960679288384 22.797226136201715 50-51 22.738953293555692 26.91070699189669 27.513098034724386 22.837241679823233 52-53 23.04503717363601 26.77593391532549 27.465229335285684 22.713799575752816 54-55 22.57477147180768 26.770071502788866 27.678077511125824 22.97707951427763 56-57 22.627482191213332 27.06987021272122 27.543861532797116 22.758786063268335 58-59 22.64735409542114 26.56769242332326 27.67140533314722 23.113548148108382 60-61 22.623869493256947 26.66607910477041 27.840032247305697 22.870019154666945 62-63 22.64241511101826 26.736201591186802 27.800228641675638 22.821154656119297 64-65 22.366159213837694 26.577512918204576 27.7571008464357 23.29922702152203 66-67 22.318472842055538 26.91017765307547 27.595657627737356 23.17569187713164 68-69 22.578066264641166 26.546053050992402 27.779419393096433 23.096461291270003 70-71 22.35575530209153 26.700921074738577 27.61915457818061 23.32416904498928 72-73 22.61055673103123 26.664956025179094 27.508994290454396 23.215492953335282 74-75 22.50724271310355 26.80651721137089 27.452488920606445 23.233751154919116 76-77 22.55703952585527 26.69312237367642 27.489880580221076 23.25995752024723 78-79 22.649617576494766 26.853614357310647 27.365413633396845 23.131354432797743 80-81 22.534558810371667 27.08265483241773 27.267169801191777 23.115616556018825 82-83 22.769969118777958 26.954032475611083 27.136376182762433 23.139622222848523 84-85 22.771363909048574 26.70390771544642 27.20796815885856 23.316760216646454 86-87 22.83604872038746 26.69698642589807 27.194327705716404 23.27263714799806 88-89 22.71424023239679 26.707016314893718 27.164977066165026 23.41376638654447 90-91 22.707283761783767 26.803801235276165 27.228634385284085 23.260280617655983 92-93 22.98344897262465 26.484753466158562 27.16590446301892 23.36589309819787 94-95 22.749987191015407 26.49387999021819 27.18482682364702 23.571305995119385 96-97 23.044063309543105 26.25567701017286 27.12934029215039 23.570919388133646 98-99 22.79818038721582 26.361476898461326 26.885799029164005 23.954543685158843 100-101 23.37882904308522 25.88955802248181 27.120602596453324 23.611010337979646 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 499.0 1 368.0 2 306.5 3 387.0 4 330.5 5 277.5 6 506.5 7 1191.5 8 1938.0 9 2196.0 10 1875.5 11 1571.5 12 1636.5 13 1770.0 14 1899.5 15 2034.5 16 2177.0 17 2352.0 18 2532.0 19 2790.0 20 3117.5 21 3593.5 22 4373.0 23 5168.5 24 6207.0 25 7855.5 26 10019.5 27 12799.0 28 15618.5 29 18629.0 30 22144.0 31 26056.5 32 30737.0 33 34935.0 34 38637.5 35 42787.5 36 47618.0 37 52472.5 38 56831.5 39 59978.0 40 63029.5 41 68319.5 42 73379.0 43 77672.5 44 81241.5 45 84482.5 46 86028.5 47 87348.5 48 91177.5 49 92132.5 50 89794.0 51 86722.0 52 82801.0 53 80692.0 54 78872.0 55 72735.5 56 65133.5 57 58859.5 58 53227.5 59 46519.5 60 38814.0 61 32029.0 62 26256.0 63 20510.5 64 15915.5 65 12684.5 66 9581.5 67 7318.5 68 5542.5 69 4053.0 70 3150.0 71 2472.5 72 2087.5 73 1838.5 74 1438.0 75 1144.5 76 875.0 77 577.5 78 381.5 79 235.0 80 140.0 81 92.5 82 56.0 83 43.5 84 25.0 85 10.5 86 11.5 87 11.5 88 6.5 89 3.5 90 3.5 91 3.0 92 2.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1782636449972332 2 0.047462415895122824 3 0.04652300898296677 4 0.05283045539315745 5 0.05994310772805333 6 0.06495327792621898 7 0.03542906068702854 8 0.032923975587945714 9 0.03954455763552175 10-11 0.04187070808467008 12-13 0.037732844304935065 14-15 0.03341604587526555 16-17 0.03193983501330603 18-19 0.04039449722271057 20-21 0.050571405437734536 22-23 0.047194013920221094 24-25 0.06850960409366692 26-27 0.05283045539315745 28-29 0.0451809991084581 30-31 0.053971163786489806 32-33 0.04992276733172203 34-35 0.049318862888193135 36-37 0.04694797877656117 38-39 0.025565288109389912 40-41 0.023798308441286845 42-43 0.020711685729916933 44-45 0.003958929129800537 46-47 0.010937380477245552 48-49 0.010355842864958466 50-51 0.008678330521822647 52-53 0.008924365665482567 54-55 0.010087440890056736 56-57 0.00805205924705194 58-59 0.010065074058814924 60-61 0.01017690821502398 62-63 0.003466858842480696 64-65 0.006195612253981632 66-67 0.00380236131110786 68-69 0.008432295378162726 70-71 0.022791801035405353 72-73 0.01896707289305568 74-75 0.020040680792662604 76-77 0.01210045570181972 78-79 0.0046523008982966765 80-81 0.005949577110321711 82-83 0.005099637523132895 84-85 0.00474176822326392 86-87 0.007470521634764854 88-89 0.029278182095530524 90-91 0.03627900027421735 92-93 0.02174055996704024 94-95 0.03102279493239178 96-97 0.03498172406219231 98-99 0.02111428869226953 100-101 0.12145189364303342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.72281206174448 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51194764365205 40.56714171676207 2 13.488126948887565 14.49240218280075 3 4.728461996960097 7.620788256113649 4 2.086039267504657 4.482715820862873 5 1.134798973977195 3.048229600341866 6 0.692610903051242 2.2325403225922194 7 0.4416344699079042 1.6608091938795335 8 0.30822817841634775 1.3247107600956238 9 0.21537537619329955 1.0413513772164131 >10 1.236218709262247 12.044113625003577 >50 0.09575212368796632 3.573899681699795 >100 0.056298478482773015 5.473547552833269 >500 0.0036723133468182694 1.3585451831403865 >1k 6.676933357851226E-4 0.5348271211755571 >5k 1.6692333394628065E-4 0.5443776054822635 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6845 0.30620191970039184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5296 0.23690947651326152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3761 0.16824330460090192 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2366831241810944E-5 0.0 12-13 0.0 0.0 0.0 8.946732496724378E-5 0.0 14-15 0.0 0.0 0.0 1.3420098745086566E-4 0.0 16-17 0.0 0.0 0.0 1.3420098745086566E-4 0.0 18-19 0.0 0.0 0.0 1.5656781869267662E-4 0.0 20-21 0.0 0.0 0.0 3.80236131110786E-4 0.0 22-23 0.0 0.0 0.0 5.591707810452736E-4 0.0 24-25 0.0 0.0 0.0 9.170400809142487E-4 0.0 26-27 0.0 0.0 0.0 0.001677512343135821 0.0 28-29 0.0 0.0 0.0 0.004719401392022109 0.0 30-31 0.0 0.0 0.0 0.01496341010077152 0.0 32-33 0.0 0.0 0.0 0.035496161180753966 0.0 34-35 0.0 0.0 0.0 0.06497564475746079 0.0 36-37 0.0 0.0 0.0 0.11521154772656816 0.0 38-39 0.0 0.0 0.0 0.1921087135359142 0.0 40-41 0.0 0.0 0.0 0.2763198331613324 0.0 42-43 0.0 0.0 0.0 0.3614703596989067 0.0 44-45 0.0 0.0 0.0 0.4690548179720173 0.0 46-47 0.0 0.0 0.0 0.5988495396682463 0.0 48-49 0.0 0.0 0.0 0.7307020098387218 0.0 50-51 0.0 0.0 0.0 0.874408900567357 0.0 52-53 0.0 0.0 0.0 1.0177802888273653 0.0 54-55 4.473366248362189E-5 0.0 0.0 1.1758243183820012 0.0 56-57 4.473366248362189E-5 0.0 0.0 1.3593218018898183 0.0 58-59 4.473366248362189E-5 0.0 0.0 1.564045408246114 0.0 60-61 4.473366248362189E-5 0.0 0.0 1.7847389321090623 0.0 62-63 4.473366248362189E-5 0.0 0.0 2.006148194571749 0.0 64-65 4.473366248362189E-5 0.0 0.0 2.239792113723706 0.0 66-67 4.473366248362189E-5 0.0 0.0 2.5039443906894934 0.0 68-69 4.473366248362189E-5 0.0 0.0 2.7965920106573474 0.0 70-71 4.473366248362189E-5 0.0 0.0 3.1083632713369505 0.0 72-73 4.473366248362189E-5 0.0 0.0 3.4281194907698795 0.0 74-75 4.473366248362189E-5 0.0 0.0 3.753400317519536 0.0 76-77 4.473366248362189E-5 0.0 0.0 4.105722643240542 0.0 78-79 4.473366248362189E-5 0.0 0.0 4.482469548677606 0.0 80-81 4.473366248362189E-5 0.0 0.0 4.891916761390197 0.0 82-83 4.473366248362189E-5 0.0 0.0 5.311339580836636 0.0 84-85 4.473366248362189E-5 0.0 0.0 5.738300022411565 0.0 86-87 4.473366248362189E-5 0.0 0.0 6.179508135487527 0.0 88-89 4.473366248362189E-5 0.0 0.0 6.656928148343982 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3625 0.0 58.976276 1 GTATCAA 6975 0.0 44.508446 1 ATCAACG 9380 0.0 32.52627 3 TCAACGC 9535 0.0 31.94769 4 TATCAAC 9545 0.0 31.91422 2 CAACGCA 9735 0.0 31.241825 5 AACGCAG 10100 0.0 30.153093 6 GTGGTAT 1730 0.0 26.146679 1 ACGCAGA 11675 0.0 25.96034 7 GTACATG 10000 0.0 25.711819 1 CGCAGAG 11885 0.0 25.421698 8 TGGTATC 1655 0.0 24.98178 2 TACATGG 10245 0.0 24.723923 2 ACATGGG 10165 0.0 23.936722 3 GCAGAGT 12805 0.0 23.000607 9 TAAGGTG 910 0.0 22.45533 5 GTAAGGT 820 0.0 22.022766 4 GAGTACT 6765 0.0 21.274208 12-13 AGAGTAC 12155 0.0 20.848627 10-11 AGTACTT 7305 0.0 20.579369 12-13 >>END_MODULE