FastQCFastQC Report
Thu 26 May 2016
SRR938370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235453
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.2920660823555673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.2450062694227971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40190.17978458952167636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.057.070861
GTATCAA73550.043.5176621
ATCAACG93350.034.018143
TCAACGC94850.033.430044
TATCAAC95900.033.0780872
CAACGCA97050.032.6722225
AACGCAG100550.031.4587966
ACGCAGA115800.027.2338757
CGCAGAG118150.026.49128
GTACATG97250.025.8212621
TAAGGTG10250.025.5086365
GTAAGGT7900.025.2738044
TACATGG98650.025.213772
ACATGGG98850.024.6230433
TGGTATC18550.024.6127682
GTGGTAT19500.024.3892571
GCAGAGT131050.023.6298269
GAGTACT67150.021.36047412-13
CAGAGTA126850.020.4059110-11
AGTACTT70750.020.37427712-13
Produced by FastQC (version 0.11.5)