FastQCFastQC Report
Thu 26 May 2016
SRR938369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70680.3148447425204328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55100.24544348207238043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38240.17034044926402592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36050.057.1223261
GTATCAA69000.043.6611751
ATCAACG90250.032.8627783
TATCAAC90400.032.8608252
TCAACGC92300.032.1843834
CAACGCA93400.031.754455
AACGCAG96250.031.0048036
TGGTATC15900.029.2951582
ACGCAGA112700.026.2614127
CGCAGAG112900.026.2148888
GTACATG101050.025.8034881
TACATGG102900.025.1275632
GTGGTAT19800.025.0377661
ACATGGG103600.024.3613663
GCAGAGT122200.023.4391569
GAGTACT63950.021.9476812-13
GTACTTT67750.020.753814-15
AGTACTT68100.020.64506512-13
AGAGTAC118500.020.06144510-11
CATGGGA55050.020.0307664
Produced by FastQC (version 0.11.5)