##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938369_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.245356173683113 31.0 31.0 34.0 28.0 34.0 2 31.393697136106653 31.0 31.0 34.0 28.0 34.0 3 31.45250779984641 31.0 31.0 34.0 28.0 34.0 4 35.12737848543108 37.0 35.0 37.0 32.0 37.0 5 35.13658372963621 37.0 35.0 37.0 32.0 37.0 6 35.14734359771145 37.0 35.0 37.0 32.0 37.0 7 35.06723102334341 37.0 35.0 37.0 32.0 37.0 8 35.031610982326285 37.0 35.0 37.0 32.0 37.0 9 36.53597061092709 39.0 37.0 39.0 32.0 39.0 10-11 36.39669368475257 39.0 37.0 39.0 32.0 39.0 12-13 36.23012976877531 39.0 35.0 39.0 32.0 39.0 14-15 37.2398361453168 39.0 36.5 41.0 31.5 41.0 16-17 37.07229869625411 39.0 36.0 41.0 31.0 41.0 18-19 36.92993390398571 39.0 36.0 41.0 31.0 41.0 20-21 36.92120774229414 39.0 36.0 40.5 30.5 41.0 22-23 37.02180571567043 39.0 36.0 41.0 31.0 41.0 24-25 36.94059955918172 39.0 36.0 41.0 30.5 41.0 26-27 36.76123650060849 39.0 36.0 41.0 30.0 41.0 28-29 36.565684863041646 39.0 36.0 41.0 30.0 41.0 30-31 36.31216936402075 39.0 35.5 40.0 30.0 41.0 32-33 36.02356101520057 38.0 35.0 40.0 29.0 41.0 34-35 35.76680419222813 38.0 35.0 40.0 27.5 41.0 36-37 35.47657930185361 38.0 34.5 40.0 27.0 41.0 38-39 35.16228691853058 38.0 34.0 40.0 26.0 41.0 40-41 35.05633885633138 38.0 34.0 40.0 25.5 41.0 42-43 34.82448207416224 38.0 33.5 40.0 25.0 41.0 44-45 34.51525535922057 38.0 33.0 40.0 24.0 41.0 46-47 34.11224117071641 38.0 33.0 40.0 23.5 41.0 48-49 33.73408849150703 38.0 33.0 40.0 21.5 41.0 50-51 32.956747602137455 37.0 31.5 39.5 20.0 40.5 52-53 32.98126299603192 37.0 32.0 39.0 19.5 40.0 54-55 33.048039213939404 37.0 32.0 40.0 18.5 41.0 56-57 32.71373739596493 37.0 31.0 40.0 15.5 41.0 58-59 32.26823631708269 36.0 31.0 39.0 12.0 41.0 60-61 31.776754453173304 36.0 30.5 39.0 8.0 41.0 62-63 31.177544505005976 35.0 29.5 39.0 7.0 40.0 64-65 30.666280831888585 35.0 29.0 38.5 2.0 40.0 66-67 30.096060832565673 34.5 28.0 38.0 2.0 40.0 68-69 29.469991750248116 34.0 27.5 37.5 2.0 40.0 70-71 28.813370077098654 34.0 26.0 37.0 2.0 39.0 72-73 28.14557248467203 33.5 26.0 36.0 2.0 39.0 74-75 27.41051157370699 33.0 25.0 36.0 2.0 38.5 76-77 26.64840020740197 32.5 23.0 35.0 2.0 37.5 78-79 25.816491574740436 32.0 20.5 35.0 2.0 37.0 80-81 25.002614797168356 31.0 17.5 35.0 2.0 36.5 82-83 24.238562601006006 31.0 9.5 34.5 2.0 36.0 84-85 23.454009860502573 30.0 4.5 34.0 2.0 35.5 86-87 22.68901709462626 30.0 2.0 34.0 2.0 35.0 88-89 21.892389292071506 29.0 2.0 34.0 2.0 35.0 90-91 21.04549992070973 29.0 2.0 34.0 2.0 35.0 92-93 20.136709569534005 27.0 2.0 34.0 2.0 35.0 94-95 19.207691958184625 25.5 2.0 33.5 2.0 35.0 96-97 18.11487445409984 24.5 2.0 33.0 2.0 35.0 98-99 16.840908746696982 11.0 2.0 32.5 2.0 34.5 100-101 14.04959695596628 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6560901930858094 1101 2 0.6160056499390265 1101 3 0.6051506369337041 1101 4 0.5358892385996725 1101 5 0.4836362604091846 1101 6 0.5178760390171036 1101 7 0.4749605274577249 1101 8 0.38303018790355736 1101 9 0.6045785494756544 1101 10-11 0.6242930920846632 1101 12-13 0.6901487178841563 1101 14-15 0.8233956463193053 1101 16-17 0.881482998775148 1101 18-19 0.9214465751707053 1101 20-21 1.0042821070240109 1101 22-23 0.89424035433575 1101 24-25 0.9380381601631242 1101 26-27 1.0449655810697394 1101 28-29 1.0672759660792224 1101 30-31 1.0370733371225427 1101 32-33 1.0515719857057348 1101 34-35 1.1376788782398037 1101 36-37 1.1717167841372458 1101 38-39 1.3362148295265754 1101 40-41 1.4577082525565288 1101 42-43 1.524642351110451 1101 44-45 1.5218376583453406 1101 46-47 1.7797334718776838 1101 48-49 1.7710920074437126 1101 50-51 1.8188843106437318 1101 52-53 2.0983985747763896 1101 54-55 2.202999036019442 1101 56-57 2.2743296921107046 1101 58-59 2.548482648073481 1101 60-61 2.4255451279222733 1101 62-63 2.4698263987870597 1101 64-65 2.6382227809251226 1101 66-67 2.894542831683477 1101 68-69 3.015252923351987 1101 70-71 3.0387435714969797 1101 72-73 3.305581622892305 1101 74-75 3.4720642251354725 1101 76-77 3.7508815672103744 1101 78-79 4.019455832783127 1101 80-81 4.516753787909543 1101 82-83 4.589072131596943 1101 84-85 4.877927125294576 1101 86-87 5.004044368031298 1101 88-89 5.470981796348202 1101 90-91 5.64429703880289 1101 92-93 5.7472504523504675 1101 94-95 6.086133255515794 1101 96-97 6.628698663970798 1101 98-99 6.930957458508718 1101 100-101 6.746495522966001 1102 1 0.4909920167438955 1102 2 0.4423107108435218 1102 3 0.3636674321114093 1102 4 0.3969385721369818 1102 5 0.3839308987073977 1102 6 0.43772031582214055 1102 7 0.40565317580502835 1102 8 0.5201299775043964 1102 9 0.4930548741062637 1102 10-11 0.5997819870221619 1102 12-13 0.6328152441522619 1102 14-15 0.8997205749445172 1102 16-17 0.8492769268023395 1102 18-19 0.8250120363302997 1102 20-21 0.8086296610095758 1102 22-23 0.632915256834913 1102 24-25 0.6473740021273215 1102 26-27 0.6470438439951778 1102 28-29 0.6960748232879155 1102 30-31 0.718373937723733 1102 32-33 0.6770935630438188 1102 34-35 0.7767935527370042 1102 36-37 0.8056053540388461 1102 38-39 0.8128515820284221 1102 40-41 1.267065054966082 1102 42-43 1.2542618445233131 1102 44-45 1.269254357959305 1102 46-47 1.5175303204598265 1102 48-49 1.393802004284744 1102 50-51 1.6385792815275835 1102 52-53 1.7711245651325882 1102 54-55 1.8776991824445446 1102 56-57 1.8268989857536297 1102 58-59 1.8008428339558762 1102 60-61 1.8564269169707934 1102 62-63 2.142208677574896 1102 64-65 2.182353160855545 1102 66-67 2.2881028093521643 1102 68-69 2.545362652773502 1102 70-71 2.533605778919963 1102 72-73 2.7985318602122256 1102 74-75 2.791165051831335 1102 76-77 2.9733189789231886 1102 78-79 3.336819028625211 1102 80-81 3.942546062942373 1102 82-83 4.0605776478696 1102 84-85 4.144058820468111 1102 86-87 4.381759341736373 1102 88-89 4.778751763904211 1102 90-91 5.078802501432701 1102 92-93 4.9307275617560435 1102 94-95 5.114708004834828 1102 96-97 5.371384759943808 1102 98-99 5.661137718338772 1102 100-101 5.559263911038215 1103 1 0.5316122357994075 1103 2 0.5612714680233992 1103 3 0.5172857823684538 1103 4 0.3491101860718473 1103 5 0.2971132803883023 1103 6 0.44256166272040787 1103 7 0.5300873018763781 1103 8 0.4765305879071491 1103 9 0.746004099075904 1103 10-11 0.5821367899510861 1103 12-13 0.7943442894095156 1103 14-15 0.792675039171975 1103 16-17 0.8582912743832694 1103 18-19 1.0163599571337656 1103 20-21 0.974725430357573 1103 22-23 0.7982094586904722 1103 24-25 0.8850799083679846 1103 26-27 0.9085359807261781 1103 28-29 1.078995037571616 1103 30-31 1.1047592685809136 1103 32-33 1.134842259802781 1103 34-35 1.2354867766626896 1103 36-37 1.3788429719099682 1103 38-39 1.4712137978254631 1103 40-41 1.585425926864275 1103 42-43 1.633053562600601 1103 44-45 1.73707917977098 1103 46-47 2.009208275552176 1103 48-49 2.0639469019000956 1103 50-51 2.12724816919809 1103 52-53 2.213417256562707 1103 54-55 2.256137396695131 1103 56-57 2.319703965580537 1103 58-59 2.7341972346889847 1103 60-61 2.9019090309431554 1103 62-63 3.008074425975604 1103 64-65 3.1360761015009793 1103 66-67 3.201771623984694 1103 68-69 3.302504842140536 1103 70-71 3.398815646253766 1103 72-73 3.5926822970280448 1103 74-75 3.8379235775548253 1103 76-77 3.9632033159769158 1103 78-79 4.014080065394477 1103 80-81 4.503441528062844 1103 82-83 4.707326727347581 1103 84-85 4.896503791218837 1103 86-87 5.008002277746339 1103 88-89 5.4561823461727315 1103 90-91 5.834052812331041 1103 92-93 5.872126560454021 1103 94-95 5.947197830644111 1103 96-97 5.932788861562038 1103 98-99 6.17136858652664 1103 100-101 6.307263108594263 1104 1 0.4498476109310161 1104 2 0.431625079357989 1104 3 0.37642958476273947 1104 4 0.3555352240963927 1104 5 0.34023981237670853 1104 6 0.3193867844328224 1104 7 0.30215640156202284 1104 8 0.31026774780289657 1104 9 0.4694102478762403 1104 10-11 0.48799800732354726 1104 12-13 0.45491934621114893 1104 14-15 0.5989487316055246 1104 16-17 0.6972724724593533 1104 18-19 0.7935587015147476 1104 20-21 0.6902174502831215 1104 22-23 0.6274105782414452 1104 24-25 0.7364654618254889 1104 26-27 0.6780102485890609 1104 28-29 0.7305837147238492 1104 30-31 0.8121203200075655 1104 32-33 0.9581650970321434 1104 34-35 0.8179887503265988 1104 36-37 0.8997785789141659 1104 38-39 1.0816336143894958 1104 40-41 1.1453866454961883 1104 42-43 1.1399826469183836 1104 44-45 1.1737940886287248 1104 46-47 1.0767102153495927 1104 48-49 1.374173378422718 1104 50-51 1.4294906201688136 1104 52-53 1.5324386729798292 1104 54-55 1.692966040522741 1104 56-57 1.8616764027713657 1104 58-59 1.9981364947588176 1104 60-61 2.0211458946952483 1104 62-63 2.4353102917950586 1104 64-65 2.424433572863098 1104 66-67 2.5589800504195175 1104 68-69 2.8746046286311184 1104 70-71 2.872318408630658 1104 72-73 2.868290915523513 1104 74-75 2.874785278841827 1104 76-77 3.0771155204823657 1104 78-79 3.4670161773856982 1104 80-81 3.780896058534033 1104 82-83 3.898230262783599 1104 84-85 4.275615559674382 1104 86-87 4.35237332806668 1104 88-89 4.443711762984208 1104 90-91 4.554310035865694 1104 92-93 4.775262654082077 1104 94-95 5.172955631038018 1104 96-97 5.2763727040843555 1104 98-99 5.4159522996036955 1104 100-101 5.185255213186036 1105 1 0.5535694144847056 1105 2 0.49095251292190056 1105 3 0.4003634961861451 1105 4 0.3081031521056232 1105 5 0.33421553404926385 1105 6 0.2561705246488941 1105 7 0.29466658121145173 1105 8 0.323558785708677 1105 9 0.45734950111389594 1105 10-11 0.41705221873275633 1105 12-13 0.5183687645428918 1105 14-15 0.8144434498321047 1105 16-17 0.8216910771105148 1105 18-19 0.7760693542131278 1105 20-21 0.7923035051640781 1105 22-23 0.843429107204777 1105 24-25 0.6971576415986434 1105 26-27 0.8192969599164002 1105 28-29 1.0149676412484325 1105 30-31 0.926000940647782 1105 32-33 0.8731929523373836 1105 34-35 1.0769220977301117 1105 36-37 1.1054476156773418 1105 38-39 1.0918300024788437 1105 40-41 1.2182777569065024 1105 42-43 1.4784800979123318 1105 44-45 1.5433895044205528 1105 46-47 1.614621813418097 1105 48-49 1.5469235758296804 1105 50-51 1.3410084522946661 1105 52-53 1.5292923516631518 1105 54-55 1.6976184769067473 1105 56-57 1.833205224188113 1105 58-59 1.7038709396358698 1105 60-61 2.0395046513770616 1105 62-63 2.2524593167684053 1105 64-65 2.3549165740311686 1105 66-67 2.465675239511736 1105 68-69 2.6991641472856216 1105 70-71 2.9383595893608287 1105 72-73 3.114505341333068 1105 74-75 3.126032763495026 1105 76-77 3.267267005310231 1105 78-79 3.6569591567935156 1105 80-81 4.048698800762384 1105 82-83 4.246672658157909 1105 84-85 4.4583042425241715 1105 86-87 4.500143630053845 1105 88-89 4.8450564955841955 1105 90-91 5.066307739758887 1105 92-93 5.220887042302433 1105 94-95 5.417068900788717 1105 96-97 5.850492196248116 1105 98-99 5.828184526608968 1105 100-101 5.234768791510206 1106 1 0.18347672346777344 1106 2 0.08770637565277895 1106 3 0.11319526984250672 1106 4 0.08402872333410016 1106 5 0.2563637942885322 1106 6 -0.17793261646095004 1106 7 0.14767193606709128 1106 8 0.11934697706738007 1106 9 0.06325227857648485 1106 10-11 -0.09180630469235496 1106 12-13 -0.02612256048205097 1106 14-15 0.30048215475404305 1106 16-17 0.045597934030844556 1106 18-19 0.4197846382002055 1106 20-21 0.264500012661685 1106 22-23 -0.010040655879855365 1106 24-25 0.0783601829537588 1106 26-27 0.16489057366781878 1106 28-29 0.009289533521339877 1106 30-31 0.26681812121054094 1106 32-33 0.5174724977692406 1106 34-35 0.386309570871866 1106 36-37 0.533166051395014 1106 38-39 0.49870335319874215 1106 40-41 0.5689897557227823 1106 42-43 0.24034742566779954 1106 44-45 0.40550628366415964 1106 46-47 0.803032667644203 1106 48-49 0.689097307716537 1106 50-51 0.08283496731758078 1106 52-53 0.6751807139524075 1106 54-55 0.656134730740888 1106 56-57 0.6663536777795116 1106 58-59 0.16759381246735927 1106 60-61 1.485467358924236 1106 62-63 1.7615603019565391 1106 64-65 1.6367665273271008 1106 66-67 1.8985026196266013 1106 68-69 1.351574921629151 1106 70-71 1.9595267463673025 1106 72-73 2.3882459426491316 1106 74-75 2.687203478907005 1106 76-77 2.755942833660736 1106 78-79 3.161761914920884 1106 80-81 3.6286928458797014 1106 82-83 3.8009083861831634 1106 84-85 4.052896646236171 1106 86-87 4.22286504775165 1106 88-89 4.366801348831739 1106 90-91 4.636129647009771 1106 92-93 4.870813014759285 1106 94-95 4.948889397385994 1106 96-97 5.153635666256296 1106 98-99 5.141943210434654 1106 100-101 4.529572339385737 1107 1 0.40838386464799115 1107 2 0.3174529766316674 1107 3 0.3864179194498689 1107 4 0.3842728654846397 1107 5 0.4146575307703344 1107 6 0.41270839281467175 1107 7 0.33788696861788736 1107 8 0.3606617204860285 1107 9 0.31553958255979353 1107 10-11 0.43469208986099517 1107 12-13 0.5564931794114827 1107 14-15 0.6800779410479834 1107 16-17 0.7767943019811838 1107 18-19 0.7977081177123537 1107 20-21 0.7735713887575386 1107 22-23 0.7471163690315663 1107 24-25 0.7301118024597812 1107 26-27 0.7924373958475286 1107 28-29 0.8342904931294299 1107 30-31 0.8105640205596956 1107 32-33 1.0689993458181988 1107 34-35 0.9929704114528377 1107 36-37 1.0060915349259147 1107 38-39 1.15188635090729 1107 40-41 1.2064929358193552 1107 42-43 1.3795015780035769 1107 44-45 1.4040871348856214 1107 46-47 1.2145484110191163 1107 48-49 1.5832032609465756 1107 50-51 1.5961648609394459 1107 52-53 1.75037158669587 1107 54-55 1.8571082643035126 1107 56-57 1.9626795838950315 1107 58-59 2.1452843973284033 1107 60-61 2.1833729138499756 1107 62-63 2.3198207422693606 1107 64-65 2.3964834295515125 1107 66-67 2.5805262590139186 1107 68-69 2.677096836846218 1107 70-71 2.579824912221497 1107 72-73 3.06126354855639 1107 74-75 3.019349189369592 1107 76-77 3.4014892255789597 1107 78-79 3.5421586838535077 1107 80-81 3.747841267346711 1107 82-83 3.7827479593494893 1107 84-85 3.9346009816116094 1107 86-87 4.394776024686241 1107 88-89 4.6202961522192005 1107 90-91 4.777319418212425 1107 92-93 4.9096476845514445 1107 94-95 5.119120212142356 1107 96-97 5.302549880863939 1107 98-99 5.59561627776165 1107 100-101 5.118276547110984 1108 1 0.4525491001175759 1108 2 0.4173156677987073 1108 3 0.35319004274373356 1108 4 0.3482626555613848 1108 5 0.29894793679986975 1108 6 0.335918750807636 1108 7 0.3167913388266044 1108 8 0.3814431325717891 1108 9 0.2768873612988756 1108 10-11 0.3758119961982942 1108 12-13 0.4129284010641001 1108 14-15 0.4545836938146053 1108 16-17 0.6352641908858558 1108 18-19 0.6800762000804426 1108 20-21 0.5632917349996234 1108 22-23 0.5225015009384393 1108 24-25 0.573635259026716 1108 26-27 0.51179320650769 1108 28-29 0.5188348559346565 1108 30-31 0.7302318967493093 1108 32-33 0.7551879711372607 1108 34-35 1.0331313081354736 1108 36-37 1.0981228878268325 1108 38-39 0.9133804776188086 1108 40-41 1.05868019235443 1108 42-43 1.274782716762978 1108 44-45 1.19933039534628 1108 46-47 1.2269010407630532 1108 48-49 1.4880998919301618 1108 50-51 1.5102895924554787 1108 52-53 1.5626751468255193 1108 54-55 1.7597162712593502 1108 56-57 1.887390439040665 1108 58-59 2.138808499548155 1108 60-61 2.089247493637597 1108 62-63 2.279790697891496 1108 64-65 2.512974693868866 1108 66-67 2.4520710479500103 1108 68-69 2.6250068586692805 1108 70-71 2.8860946315346965 1108 72-73 3.0215206044656178 1108 74-75 3.1318950845241815 1108 76-77 3.279225825054688 1108 78-79 3.449145938666849 1108 80-81 4.000374593793083 1108 82-83 4.299654593647428 1108 84-85 4.606023526947201 1108 86-87 4.803399794179576 1108 88-89 4.915942300039262 1108 90-91 4.888659233900064 1108 92-93 5.046802852141397 1108 94-95 5.187997163628001 1108 96-97 5.566123009654461 1108 98-99 5.651876742717768 1108 100-101 5.273762659118834 1109 1 0.5090201652842943 1109 2 0.3778826370613295 1109 3 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2311 34-35 0.18350756231607335 2311 36-37 0.3224241300628563 2311 38-39 0.3738299918726682 2311 40-41 0.2730515094431425 2311 42-43 0.521000267328354 2311 44-45 0.6953428973370421 2311 46-47 0.4735864206005971 2311 48-49 0.6737663993358041 2311 50-51 0.670372806343039 2311 52-53 0.9681696523200216 2311 54-55 0.97665766320074 2311 56-57 1.057734044710358 2311 58-59 0.918646053298751 2311 60-61 1.094642460989089 2311 62-63 1.241528235715986 2311 64-65 1.360478277242013 2311 66-67 1.0992282385854644 2311 68-69 1.1460293144743474 2311 70-71 1.5263199858904883 2311 72-73 1.4373392061537835 2311 74-75 1.6763149762266814 2311 76-77 1.8401790023122153 2311 78-79 2.108719517262166 2311 80-81 2.119882079061437 2311 82-83 2.426026229258195 2311 84-85 2.844182469453969 2311 86-87 3.001747461005504 2311 88-89 2.924233827352527 2311 90-91 3.280893267981927 2311 92-93 3.5998029317719116 2311 94-95 3.721382256795703 2311 96-97 3.5373584770855793 2311 98-99 3.7316406382425313 2311 100-101 3.522102362893321 2312 1 -0.38893490994351154 2312 2 -0.28347570156973845 2312 3 -0.20051099929722938 2312 4 -0.2692176243694888 2312 5 -0.24615156185694076 2312 6 -0.13075048246238907 2312 7 -0.27406299787753596 2312 8 -0.26070990543104955 2312 9 -0.2578148271582492 2312 10-11 -0.2617100338138485 2312 12-13 -0.3065495518130632 2312 14-15 -0.16438607159784624 2312 16-17 0.00409065427753319 2312 18-19 -0.05337519700731974 2312 20-21 -0.011237721051571725 2312 22-23 -0.16886187876485792 2312 24-25 -0.2571139324932261 2312 26-27 -0.01887813137708605 2312 28-29 -0.08336985634910832 2312 30-31 -0.0600984251027441 2312 32-33 -0.01582780491803959 2312 34-35 -0.038751552087305186 2312 36-37 0.10335747537367013 2312 38-39 0.28577580297855576 2312 40-41 0.35455009978560525 2312 42-43 0.2574585718696696 2312 44-45 0.2892188336536847 2312 46-47 0.11940270905523676 2312 48-49 0.48578096579700514 2312 50-51 0.5694776047067265 2312 52-53 0.5088714406502746 2312 54-55 0.754090704467778 2312 56-57 0.79068168007894 2312 58-59 0.8447848354651981 2312 60-61 0.9258442631395063 2312 62-63 1.073226182038436 2312 64-65 1.031603101034822 2312 66-67 1.2548737788159876 2312 68-69 1.3616233054635956 2312 70-71 1.5896232833835064 2312 72-73 1.701565691131261 2312 74-75 1.8095134477156698 2312 76-77 2.072398660124758 2312 78-79 2.126068324581329 2312 80-81 2.4176974553847153 2312 82-83 2.6086753489132413 2312 84-85 2.9226084900736637 2312 86-87 3.1445651176571516 2312 88-89 3.1845433301027413 2312 90-91 3.5350997828109705 2312 92-93 3.6857198310781385 2312 94-95 3.654143665347629 2312 96-97 3.6036009796423123 2312 98-99 3.6994607134242656 2312 100-101 3.2816023490731503 2313 1 -0.25337134391572746 2313 2 -0.34017519163479903 2313 3 -0.37939090082703686 2313 4 -0.16878857566190675 2313 5 -0.1309115407561876 2313 6 -0.20901142847962006 2313 7 -0.16606932810436348 2313 8 -0.14445704803699755 2313 9 -0.30620589450483493 2313 10-11 -0.17740372117098246 2313 12-13 -0.3295071744612059 2313 14-15 -0.2827402126212277 2313 16-17 -0.17783391051937514 2313 18-19 -0.04891062869362628 2313 20-21 -0.07875222782139701 2313 22-23 -0.19248065843779472 2313 24-25 -0.08599471322120422 2313 26-27 -0.09419815951142851 2313 28-29 -0.13824588940696003 2313 30-31 0.051419950139028 2313 32-33 -0.04283061835219115 2313 34-35 0.02459291775620187 2313 36-37 0.2150675158167843 2313 38-39 0.2295264623771871 2313 40-41 0.2656728358503173 2313 42-43 0.2575561628666705 2313 44-45 0.23618559996281618 2313 46-47 0.26111274949834495 2313 48-49 0.4289311328446672 2313 50-51 0.7943087986578519 2313 52-53 0.7472132728992591 2313 54-55 0.7807717313712388 2313 56-57 0.972691527098462 2313 58-59 1.0405040195692976 2313 60-61 0.9948498900077958 2313 62-63 1.171075663311516 2313 64-65 1.3169961026173773 2313 66-67 1.3236991700591716 2313 68-69 1.3354522134994653 2313 70-71 1.4126855306140307 2313 72-73 1.4905765931885817 2313 74-75 1.4255658479266735 2313 76-77 1.5436014032230503 2313 78-79 1.758729657445759 2313 80-81 2.119320455208875 2313 82-83 2.4150618444293315 2313 84-85 2.7852733397307787 2313 86-87 2.910395080730826 2313 88-89 2.8437344677851755 2313 90-91 3.018438098267275 2313 92-93 2.974535551141443 2313 94-95 3.2826912299686946 2313 96-97 3.4556732497319906 2313 98-99 3.3984760114724466 2313 100-101 3.1426406821134165 2314 1 -0.48365331963823976 2314 2 -0.4771005838685234 2314 3 -0.5600965160099278 2314 4 -0.2642470452970258 2314 5 -0.400280992935663 2314 6 -0.1696860434552434 2314 7 -0.38877031127954353 2314 8 -0.29635788342049096 2314 9 -0.41139031870302034 2314 10-11 -0.30014066098191705 2314 12-13 -0.4977553022829895 2314 14-15 -0.4449106777144536 2314 16-17 -0.30177454151777994 2314 18-19 -0.35999761851942935 2314 20-21 -0.2852885854772822 2314 22-23 -0.31461273373946597 2314 24-25 -0.16839740951829896 2314 26-27 -0.2327107803806996 2314 28-29 -0.29009256454460797 2314 30-31 -0.27820804737374516 2314 32-33 -0.22462214195831365 2314 34-35 -0.2092530905165333 2314 36-37 -0.12568532061206383 2314 38-39 -0.03271195084476375 2314 40-41 0.040825088321767566 2314 42-43 0.1968851525663169 2314 44-45 0.18407235908939867 2314 46-47 0.1198601248441662 2314 48-49 0.24879766974301987 2314 50-51 0.3842676152429547 2314 52-53 0.35522646601072694 2314 54-55 0.28531127916472343 2314 56-57 0.437487908004492 2314 58-59 0.9094575823402167 2314 60-61 0.840372793213259 2314 62-63 1.0206929369153848 2314 64-65 1.032003897727634 2314 66-67 1.371743994196077 2314 68-69 1.4840464802634585 2314 70-71 1.6696080391075476 2314 72-73 1.5153967649513582 2314 74-75 1.615701293599713 2314 76-77 1.9663646571634104 2314 78-79 2.0847056683318783 2314 80-81 2.217379968397484 2314 82-83 2.503946648019589 2314 84-85 2.966648433227043 2314 86-87 3.0446971705413155 2314 88-89 3.0252364458507266 2314 90-91 3.1746947331226814 2314 92-93 3.2574618265206077 2314 94-95 3.536491458865573 2314 96-97 3.4846642110087664 2314 98-99 3.6467286451383103 2314 100-101 3.406334392856248 2315 1 -0.5677718552845512 2315 2 -0.44939809365287786 2315 3 -0.4076422020780619 2315 4 -0.45124862497247165 2315 5 -0.4317845203927462 2315 6 -0.3731918686220723 2315 7 -0.4326732912127582 2315 8 -0.5107560161677611 2315 9 -0.5696156287756722 2315 10-11 -0.35974323354428606 2315 12-13 -0.48521624212626335 2315 14-15 -0.4926625941388991 2315 16-17 -0.411525810802452 2315 18-19 -0.4730609346017616 2315 20-21 -0.37076756888614426 2315 22-23 -0.5190480952015619 2315 24-25 -0.5933082005739578 2315 26-27 -0.38442188337226213 2315 28-29 -0.4660570482052435 2315 30-31 -0.4111031071160127 2315 32-33 -0.38619012889917315 2315 34-35 -0.4231944989180789 2315 36-37 -0.22563287684141642 2315 38-39 0.00314713628824137 2315 40-41 -0.0029455551642030287 2315 42-43 0.024445944621767524 2315 44-45 0.013059858021442494 2315 46-47 0.08504754997318997 2315 48-49 0.03870101687351735 2315 50-51 0.3495622001736862 2315 52-53 0.28155260264083637 2315 54-55 0.3884440900599415 2315 56-57 0.4464455221674015 2315 58-59 0.8568915640857782 2315 60-61 0.9168907995137765 2315 62-63 1.0094319778033416 2315 64-65 1.0563769810759673 2315 66-67 1.170824546737098 2315 68-69 1.242646077073566 2315 70-71 1.2555501091913328 2315 72-73 1.7167760270302388 2315 74-75 1.8056206374436137 2315 76-77 1.9630754238604382 2315 78-79 2.3115549702025824 2315 80-81 2.6365709346171506 2315 82-83 2.826858940584934 2315 84-85 2.988295894748582 2315 86-87 3.1231245118330087 2315 88-89 3.2671241125664743 2315 90-91 3.308588769438856 2315 92-93 3.267599198296953 2315 94-95 3.2988267427072557 2315 96-97 3.304172930126473 2315 98-99 3.084879079685397 2315 100-101 2.9091597214705516 2316 1 -0.7812161453112978 2316 2 -0.6485260839581706 2316 3 -0.654143784584889 2316 4 -0.5355000626715238 2316 5 -0.48710899854110323 2316 6 -0.3876327129156607 2316 7 -0.4961364606151193 2316 8 -0.4789414017518254 2316 9 -0.5747065364288417 2316 10-11 -0.5154355135600213 2316 12-13 -0.4933045224820276 2316 14-15 -1.0499057506123535 2316 16-17 -0.7268408393785109 2316 18-19 -0.41247276791762744 2316 20-21 -0.3649760485922968 2316 22-23 -0.7124825205074359 2316 24-25 -0.6699130957687132 2316 26-27 -0.5256262689227214 2316 28-29 -0.30158536430332106 2316 30-31 -0.18899901553028542 2316 32-33 -0.1746696571322417 2316 34-35 -0.3145986500084419 2316 36-37 -0.18989362572990842 2316 38-39 -0.0689771196567861 2316 40-41 -0.15705107802406104 2316 42-43 0.24586158148932924 2316 44-45 0.09331242650612381 2316 46-47 0.3203248000290415 2316 48-49 0.4370892519583123 2316 50-51 0.5234321558714115 2316 52-53 0.6626302294934376 2316 54-55 0.70548178752734 2316 56-57 0.7411986372643824 2316 58-59 0.8596369811420672 2316 60-61 0.7605823204006974 2316 62-63 1.2424544768791428 2316 64-65 1.3597548077929495 2316 66-67 1.530426043644244 2316 68-69 1.6841704235605235 2316 70-71 1.3176378518308454 2316 72-73 1.7389758993226323 2316 74-75 2.1857789078951164 2316 76-77 2.383399417668791 2316 78-79 2.2970381141820404 2316 80-81 2.550968484845189 2316 82-83 2.8080769271934294 2316 84-85 2.5442004936063327 2316 86-87 2.960304427340393 2316 88-89 3.198067013822399 2316 90-91 3.427883985543456 2316 92-93 3.4560891052443665 2316 94-95 3.6405364233789257 2316 96-97 3.646593602153171 2316 98-99 3.880583972310184 2316 100-101 3.5748054850770465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6388.0 3 2087.0 4 2254.0 5 2604.0 6 2980.0 7 3904.0 8 4836.0 9 6416.0 10 8075.0 11 10550.0 12 13283.0 13 16078.0 14 18577.0 15 22318.0 16 25700.0 17 30412.0 18 29905.0 19 28446.0 20 30899.0 21 33824.0 22 37923.0 23 43016.0 24 48158.0 25 54418.0 26 60752.0 27 68688.0 28 76576.0 29 86092.0 30 97231.0 31 110976.0 32 126333.0 33 145576.0 34 171174.0 35 200936.0 36 232662.0 37 241032.0 38 134138.0 39 9699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.421930888188555 18.751714481527383 14.458518705831159 23.367835924452905 2 15.433284540781884 19.930442764554773 40.43730602732828 24.19896666733506 3 19.222463454240486 24.325634704612582 29.394804717927876 27.05709712321906 4 12.80138683398991 16.42119289113888 36.6844328775914 34.09298739727981 5 14.095085961028387 37.33253165736107 33.67578387374406 14.896598507866482 6 32.57085960363014 35.48618285641955 17.498937005663063 14.44402053428725 7 29.197829319338602 32.39993530198866 20.244996932191405 18.157238446481333 8 26.64170445309492 35.0422334630905 19.528854928633557 18.78720715518102 9 26.80176145493825 16.805522505251655 19.87661615986068 36.516099879949415 10-11 25.25796761001553 26.543372155093365 27.079447518440265 21.119212716450843 12-13 26.112483034149147 23.7856104631733 27.576158066922318 22.525748435755236 14-15 22.931219085971797 25.14112235772919 25.87668741971431 26.0509711365847 16-17 22.672714684279043 28.53887890390745 26.313710691375558 22.474695720437946 18-19 22.543274564581022 26.87765940856981 28.82186251052625 21.75720351632292 20-21 23.300287530788964 26.07174567172027 28.77326509536642 21.85470170212434 22-23 22.95427987715286 26.03403583152965 28.56766339794653 22.444020893370954 24-25 22.67197555203026 26.176610910445763 28.8094383353489 22.341975202175075 26-27 22.337055748888243 26.66045892709048 28.487486257633226 22.514999066388054 28-29 22.347305602684315 26.76210008477607 28.747018634024723 22.143575678514893 30-31 22.407334501267414 26.35596322589543 28.654467303304372 22.582234969532784 32-33 22.125923589073153 26.493917300958813 28.587371013896057 22.792788096071977 34-35 22.903197059151164 26.562437339868556 28.26082210092459 22.2735435000557 36-37 22.813516335104804 26.241479995562045 28.25497922047947 22.690024448853684 38-39 22.46437393672037 26.56235972839742 28.40359193318689 22.569674401695323 40-41 22.754692838656705 26.34536654998336 28.400400617142868 22.49953999421707 42-43 22.60991291884596 26.328733305695597 28.211790027736498 22.849563747721945 44-45 22.529829597027852 26.6027415140986 28.214554361958356 22.652874526915188 46-47 22.66244367719971 26.702827016410268 27.73504320151194 22.899686104878082 48-49 22.711634020938916 26.76609596509631 27.699412339031436 22.822857674933335 50-51 22.734884342528762 26.89436580503614 27.55545656023432 22.815293292200778 52-53 23.02672711701508 26.7971895265428 27.474237883480505 22.701845472961608 54-55 22.559779469758947 26.76684464320756 27.701728215989913 22.97164767104358 56-57 22.694980013872016 27.047269542548648 27.51967095612229 22.73807948745705 58-59 22.68972793914397 26.529358084457073 27.664327826393176 23.11658615000578 60-61 22.616959445327602 26.689243444081157 27.857039283726344 22.836757826864897 62-63 22.664968530807332 26.75515059262135 27.77492305043468 22.804957826136636 64-65 22.358951559265357 26.583043136786706 27.807971781179404 23.250033522768533 66-67 22.39720196733609 26.905695358692267 27.56493380665101 23.132168867320637 68-69 22.60418585474844 26.60066746125279 27.762565905840432 23.03258077815834 70-71 22.417370973799375 26.687987076775304 27.58474517751391 23.309896771911408 72-73 22.639649653507142 26.661216634330305 27.516611832213258 23.182521879949302 74-75 22.54107517032934 26.837448553712516 27.385006117756284 23.236470158201858 76-77 22.60093365889776 26.706818721469123 27.464041124114978 23.228206495518144 78-79 22.692007166830155 26.849597244458938 27.335991047698975 23.122404541011925 80-81 22.66957241099116 27.044359970758425 27.207095314442732 23.078972303807685 82-83 22.87803841429757 26.91774111848129 27.094006013455207 23.11021445376594 84-85 22.86986676205671 26.63522685687061 27.167252051780576 23.3276543292921 86-87 22.871072891018166 26.69326036770135 27.21110253609026 23.224564205190227 88-89 22.80837328248356 26.673943649421705 27.200938285245847 23.316744782848893 90-91 22.80315516363301 26.767850944673242 27.201523311504904 23.227470580188843 92-93 23.05885272306919 26.46092668085005 27.166828793232607 23.313391802848155 94-95 22.852625707185062 26.445201567235266 27.179185925179393 23.522986800400275 96-97 23.12195706566679 26.291175401328733 27.088375188902514 23.498492344101965 98-99 22.99036150482593 26.292219927872786 26.827627531302277 23.88979103599901 100-101 23.481953603292972 25.849367774691324 27.169579698871104 23.499098923144597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 546.0 1 367.5 2 297.0 3 398.0 4 332.0 5 310.0 6 553.5 7 1220.0 8 1983.0 9 2308.5 10 1991.0 11 1690.5 12 1748.0 13 1825.5 14 1986.5 15 2141.0 16 2317.0 17 2462.5 18 2608.5 19 2884.5 20 3232.0 21 3782.5 22 4489.5 23 5288.5 24 6434.5 25 8105.0 26 10281.0 27 13079.0 28 15830.5 29 18843.5 30 22485.5 31 26411.5 32 31119.0 33 35084.5 34 38659.5 35 42792.0 36 47480.0 37 52344.5 38 56658.0 39 60100.0 40 63554.5 41 68603.0 42 73372.5 43 77710.5 44 81164.5 45 84095.0 46 85368.0 47 86530.0 48 90196.5 49 91583.5 50 89849.5 51 86606.0 52 82936.5 53 80907.5 54 78508.0 55 72401.5 56 65133.5 57 59134.5 58 53766.0 59 47219.0 60 39693.5 61 32888.5 62 26915.5 63 21232.5 64 16573.0 65 13175.0 66 10084.5 67 7780.0 68 5948.5 69 4360.5 70 3330.0 71 2644.5 72 2155.5 73 1847.5 74 1495.0 75 1180.5 76 921.0 77 625.0 78 419.5 79 297.5 80 191.0 81 113.0 82 66.5 83 44.0 84 27.5 85 17.0 86 11.5 87 10.0 88 7.5 89 6.5 90 4.0 91 1.5 92 2.0 93 1.5 94 1.5 95 1.5 96 1.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.295333990225024 2 0.0321170146232643 3 0.03919968497707709 4 0.04258511231600648 5 0.05715135889271581 6 0.05585955109233486 7 0.028642497091205196 8 0.03225064991295888 9 0.038397873238909606 10-11 0.028597951994640332 12-13 0.031270657788531955 14-15 0.026147971683573017 16-17 0.019332571909149384 18-19 0.023430720793116533 20-21 0.036014710572689575 22-23 0.029555671570784835 24-25 0.050358231666574604 26-27 0.042006026060663296 28-29 0.034232906710095164 30-31 0.03485653806200321 32-33 0.030379755857234746 34-35 0.029667034312196982 36-37 0.028464316704945754 38-39 0.017216679822318517 40-41 0.01688259159808207 42-43 0.017840311174226565 44-45 0.00924310753720852 46-47 0.016771228856669915 48-49 0.015123060283770083 50-51 0.006280858615645308 52-53 0.004387692011638743 54-55 0.0064367664536223185 56-57 0.004855415525569777 58-59 0.006347676260492597 60-61 0.005857680198279133 62-63 0.004521327301333323 64-65 0.007706301705720838 66-67 0.0023608901179375977 68-69 0.006303131163927737 70-71 0.012249901555336591 72-73 0.012517172134725755 74-75 0.009822193792551703 76-77 0.008864474216407205 78-79 0.005790862553431843 80-81 0.007439031126331676 82-83 0.012606262327855474 84-85 0.004343146915073882 86-87 0.0010468097692742178 88-89 0.017639858239684694 90-91 0.026504332456091895 92-93 0.01204944862079472 94-95 0.02022347384044659 96-97 0.016570775922128047 98-99 0.01207172116907715 100-101 0.10341144167532326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2244916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51451326543432 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22584108034302 40.2567427039747 2 13.707907710499542 14.671440180297543 3 4.659784122269658 7.480982376757794 4 2.1711073193616253 4.647430057706369 5 1.162528544234802 3.1106074600949674 6 0.672487793432382 2.1592714165487914 7 0.45319034019260707 1.6976582330402774 8 0.3245769295289536 1.3895661120744904 9 0.23642278799677457 1.1386845382053925 >10 1.231378644181194 12.036820361033767 >50 0.09443874810134005 3.52506720092604 >100 0.05564010156847017 5.382400983475099 >500 0.0037734736256923172 1.3492888340145412 >1k 7.54694725138444E-4 0.5896070902659534 >5k 1.6770993891965423E-4 0.5644324515842273 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7068 0.3148447425204328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5510 0.24544348207238043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3824 0.17034044926402592 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.454509656486033E-5 0.0 8 0.0 0.0 0.0 4.454509656486033E-5 0.0 9 0.0 0.0 0.0 4.454509656486033E-5 0.0 10-11 0.0 0.0 0.0 8.909019312972066E-5 0.0 12-13 0.0 0.0 0.0 8.909019312972066E-5 0.0 14-15 0.0 0.0 0.0 8.909019312972066E-5 0.0 16-17 0.0 0.0 0.0 8.909019312972066E-5 0.0 18-19 0.0 0.0 0.0 1.7818038625944132E-4 0.0 20-21 0.0 0.0 0.0 2.67270579389162E-4 0.0 22-23 0.0 0.0 0.0 4.454509656486033E-4 0.0 24-25 0.0 0.0 0.0 8.686293830147765E-4 0.0 26-27 0.0 0.0 0.0 0.0016258960246174022 0.0 28-29 0.0 0.0 0.0 0.005033595911829217 0.0 30-31 0.0 0.0 0.0 0.015479421056288967 0.0 32-33 0.0 0.0 0.0 0.036393343893490894 0.0 34-35 0.0 0.0 0.0 0.06842126832362547 0.0 36-37 0.0 0.0 0.0 0.11978176466290943 0.0 38-39 0.0 0.0 0.0 0.19644387585103407 0.0 40-41 0.0 0.0 0.0 0.27878548685117843 0.0 42-43 0.0 0.0 0.0 0.3650916114455953 0.0 44-45 0.0 0.0 0.0 0.4719998432012601 0.0 46-47 0.0 0.0 0.0 0.5984410998006162 0.0 48-49 0.0 0.0 0.0 0.735639997220386 0.0 50-51 0.0 0.0 0.0 0.879119753255801 0.0 52-53 0.0 0.0 0.0 1.024292222960681 0.0 54-55 0.0 0.0 0.0 1.1841200294354 0.0 56-57 0.0 0.0 0.0 1.3672226488652581 0.0 58-59 0.0 0.0 0.0 1.5650696952580854 0.0 60-61 0.0 0.0 0.0 1.783964299777809 0.0 62-63 0.0 0.0 0.0 1.9986271201238712 0.0 64-65 0.0 2.2272548282430165E-5 0.0 2.227945277239772 0.0 66-67 0.0 4.454509656486033E-5 0.0 2.484146400132566 0.0 68-69 0.0 4.454509656486033E-5 0.0 2.7698141044030153 0.0 70-71 0.0 4.454509656486033E-5 0.0 3.077509358924788 0.0 72-73 0.0 4.454509656486033E-5 0.0 3.393690454342167 0.0 74-75 0.0 4.454509656486033E-5 0.0 3.719426472972708 0.0 76-77 0.0 4.454509656486033E-5 0.0 4.059149651924615 0.0 78-79 0.0 4.454509656486033E-5 0.0 4.434018912066198 2.2272548282430165E-5 80-81 0.0 4.454509656486033E-5 0.0 4.842965171079898 1.33635289694581E-4 82-83 4.454509656486033E-5 4.454509656486033E-5 0.0 5.264272694390347 1.33635289694581E-4 84-85 4.454509656486033E-5 4.454509656486033E-5 0.0 5.680012080630188 1.33635289694581E-4 86-87 4.454509656486033E-5 4.454509656486033E-5 0.0 6.111832246729945 1.33635289694581E-4 88-89 4.454509656486033E-5 4.454509656486033E-5 0.0 6.5764153313531555 1.33635289694581E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3605 0.0 57.122326 1 GTATCAA 6900 0.0 43.661175 1 ATCAACG 9025 0.0 32.862778 3 TATCAAC 9040 0.0 32.860825 2 TCAACGC 9230 0.0 32.184383 4 CAACGCA 9340 0.0 31.75445 5 AACGCAG 9625 0.0 31.004803 6 TGGTATC 1590 0.0 29.295158 2 ACGCAGA 11270 0.0 26.261412 7 CGCAGAG 11290 0.0 26.214888 8 GTACATG 10105 0.0 25.803488 1 TACATGG 10290 0.0 25.127563 2 GTGGTAT 1980 0.0 25.037766 1 ACATGGG 10360 0.0 24.361366 3 GCAGAGT 12220 0.0 23.439156 9 GAGTACT 6395 0.0 21.94768 12-13 GTACTTT 6775 0.0 20.7538 14-15 AGTACTT 6810 0.0 20.645065 12-13 AGAGTAC 11850 0.0 20.061445 10-11 CATGGGA 5505 0.0 20.030766 4 >>END_MODULE