FastQCFastQC Report
Thu 26 May 2016
SRR938369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66660.29693761370135896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55710.24816073296283692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.17639858239684691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.055.359551
GTATCAA71200.041.4842641
ATCAACG93350.031.6289233
TCAACGC95650.030.8683744
TATCAAC96100.030.8344672
CAACGCA97700.030.2206765
AACGCAG100750.029.4159016
ACGCAGA117700.024.9375977
TGGTATC18950.024.8483622
CGCAGAG118300.024.8111178
GTACATG94550.024.246961
GTGGTAT21750.023.3989471
TACATGG96800.023.1428762
ACATGGG97750.022.033763
GCAGAGT130500.022.0184869
GAGTACT68900.021.50690812-13
AGTACTT73450.020.43327112-13
AGAGTAC123000.020.07894110-11
GTACTTT73550.020.0503314-15
CATGGGG45400.019.897794
Produced by FastQC (version 0.11.5)