##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938369_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.731864800286512 31.0 31.0 34.0 30.0 34.0 2 31.970177057849828 31.0 31.0 34.0 30.0 34.0 3 32.032387403359415 33.0 31.0 34.0 30.0 34.0 4 35.71859258876501 37.0 35.0 37.0 33.0 37.0 5 35.588335153742946 37.0 35.0 37.0 33.0 37.0 6 35.62682300807692 37.0 35.0 37.0 33.0 37.0 7 35.5597697196688 37.0 35.0 37.0 33.0 37.0 8 35.546575462066286 37.0 35.0 37.0 33.0 37.0 9 37.103214552348504 39.0 37.0 39.0 33.0 39.0 10-11 37.031726799577356 39.0 37.0 39.0 33.0 39.0 12-13 36.91267490632166 39.0 37.0 39.0 33.0 39.0 14-15 37.93242887484432 40.0 37.0 41.0 33.0 41.0 16-17 37.844867023977734 40.0 37.0 41.0 32.5 41.0 18-19 37.8378805710325 40.0 37.0 41.0 32.5 41.0 20-21 37.84082477918996 40.0 37.0 41.0 32.5 41.0 22-23 37.865725265444226 40.0 37.0 41.0 32.0 41.0 24-25 37.78574075822881 40.0 37.0 41.0 32.0 41.0 26-27 37.60808199505015 40.0 37.0 41.0 32.0 41.0 28-29 37.46737183039366 39.0 36.5 41.0 32.0 41.0 30-31 37.226362589958825 39.0 36.0 41.0 31.0 41.0 32-33 37.008711907260675 39.0 36.0 40.0 31.0 41.0 34-35 36.83223470276839 39.0 35.0 40.0 30.0 41.0 36-37 36.837480778790834 39.0 35.0 40.0 30.0 41.0 38-39 36.69292949045755 39.0 35.0 40.0 30.0 41.0 40-41 36.51507227887369 39.0 35.0 40.0 30.0 41.0 42-43 36.28464962608846 38.5 35.0 40.0 30.0 41.0 44-45 35.988517833183955 38.0 35.0 40.0 29.5 41.0 46-47 35.61057340230102 38.0 34.5 40.0 27.5 41.0 48-49 35.20698636385504 38.0 34.0 40.0 27.0 41.0 50-51 34.836481855000365 38.0 33.5 40.0 26.0 41.0 52-53 34.31604567832382 38.0 33.0 40.0 24.0 41.0 54-55 33.975035591532155 37.0 33.0 40.0 23.5 41.0 56-57 33.59356007084452 37.0 32.0 40.0 23.0 41.0 58-59 33.10780158366727 36.5 31.5 39.0 22.0 40.5 60-61 32.6759246225694 36.0 31.0 39.0 20.0 40.0 62-63 32.17582795970985 35.5 31.0 39.0 18.5 40.0 64-65 31.65825313731115 35.0 30.0 38.0 17.0 40.0 66-67 31.13523089505353 35.0 29.0 38.0 13.0 40.0 68-69 30.541153210186927 34.0 29.0 37.5 8.0 39.5 70-71 29.943496994987783 34.0 28.0 37.0 7.5 39.0 72-73 29.328719426472972 34.0 27.5 36.0 4.5 39.0 74-75 28.67422789984124 33.0 26.0 36.0 2.0 38.5 76-77 27.41484959793596 31.5 25.5 34.5 2.0 36.0 78-79 28.030201798196458 33.0 26.0 35.0 2.0 37.0 80-81 27.678881748804855 33.0 26.0 35.0 2.0 36.5 82-83 27.145617920670528 32.0 25.5 35.0 2.0 36.0 84-85 26.515290995297818 32.0 24.5 35.0 2.0 36.0 86-87 25.812461579854215 31.5 22.5 34.0 2.0 35.0 88-89 25.016115525035236 31.0 19.5 34.0 2.0 35.0 90-91 24.42078233662195 31.0 17.5 34.0 2.0 35.0 92-93 23.583212244912502 30.5 7.5 34.0 2.0 35.0 94-95 22.727355945612217 30.0 2.0 34.0 2.0 35.0 96-97 21.687580960713007 29.0 2.0 34.0 2.0 35.0 98-99 20.451309536748816 29.0 2.0 34.0 2.0 35.0 100-101 17.383741307024405 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32985896578544427 1101 2 0.3870810409055103 1101 3 0.3882882597548303 1101 4 0.3207954496861305 1101 5 0.30372991856781084 1101 6 0.31808824320132345 1101 7 0.34999671317739 1101 8 0.3313176388813659 1101 9 0.4312947430046705 1101 10-11 0.4335224244396443 1101 12-13 0.4787449836829012 1101 14-15 0.6884913788653435 1101 16-17 0.6882738874120946 1101 18-19 0.6758347748945326 1101 20-21 0.6913356293569421 1101 22-23 0.7501293798866158 1101 24-25 0.7391805002370901 1101 26-27 0.7192541088217013 1101 28-29 0.6162499271326922 1101 30-31 0.7025835634712223 1101 32-33 0.6285268684448653 1101 34-35 0.5959388064938977 1101 36-37 0.7501431373371688 1101 38-39 0.8598721783665155 1101 40-41 0.9437968813313304 1101 42-43 0.8711024552873639 1101 44-45 0.9597903270065942 1101 46-47 1.1421476711714504 1101 48-49 1.196046777092043 1101 50-51 1.3215385011245928 1101 52-53 1.3951675096431941 1101 54-55 1.3294835877302518 1101 56-57 1.466727444672209 1101 58-59 1.5655101887526612 1101 60-61 1.6083200288333046 1101 62-63 1.866327995258736 1101 64-65 1.9275675341276894 1101 66-67 2.0124469425690847 1101 68-69 2.0372212030699366 1101 70-71 2.220894249333192 1101 72-73 2.135973025944768 1101 74-75 2.1483909750817034 1101 76-77 2.087782063553618 1101 78-79 2.398800948604734 1101 80-81 2.7809592144904265 1101 82-83 2.893953001915829 1101 84-85 3.1213058690399222 1101 86-87 3.236941732508768 1101 88-89 3.270374660662629 1101 90-91 3.8326638120174223 1101 92-93 4.351242872470724 1101 94-95 4.1854268257082765 1101 96-97 4.5646810354266805 1101 98-99 4.988581483288872 1101 100-101 5.31695156119914 1102 1 0.34627089515967313 1102 2 0.3224819417079665 1102 3 0.3124686666060157 1102 4 0.1991549322512114 1102 5 0.1524449737305389 1102 6 0.13375533172592924 1102 7 0.1470199325825945 1102 8 0.2998747853998438 1102 9 0.3588872322115293 1102 10-11 0.35652278801893544 1102 12-13 0.2702276918121811 1102 14-15 0.5593992740988725 1102 16-17 0.45839253744021136 1102 18-19 0.46023801810126486 1102 20-21 0.4423110351054689 1102 22-23 0.4112383165860791 1102 24-25 0.4565488616865565 1102 26-27 0.5286447856950787 1102 28-29 0.37050887108386377 1102 30-31 0.4265349948411554 1102 32-33 0.2762596634954164 1102 34-35 0.5029643305546969 1102 36-37 0.6442341791805788 1102 38-39 0.8310790827968191 1102 40-41 0.9478615189739159 1102 42-43 0.7864891109340704 1102 44-45 0.7520724128788601 1102 46-47 0.8636374919447363 1102 48-49 1.022680052742757 1102 50-51 1.209089983355895 1102 52-53 1.254792029589396 1102 54-55 1.2079037661821204 1102 56-57 1.3069426915584401 1102 58-59 1.2541371107062886 1102 60-61 1.3139522296403285 1102 62-63 1.5896662094287919 1102 64-65 1.4679920874516625 1102 66-67 1.5476744975082966 1102 68-69 1.777649332583639 1102 70-71 1.9638918711670215 1102 72-73 1.874114579481322 1102 74-75 1.9985311418513518 1102 76-77 2.051470227756358 1102 78-79 2.4922079448239067 1102 80-81 2.727126631825808 1102 82-83 2.6536036479147675 1102 84-85 2.8322620229670754 1102 86-87 2.7248348401977722 1102 88-89 3.090712153634424 1102 90-91 3.3203223812633844 1102 92-93 3.7050142625555367 1102 94-95 3.8880054571799363 1102 96-97 4.06836510687895 1102 98-99 4.368403329437214 1102 100-101 4.675943989610776 1103 1 0.3616349700167838 1103 2 0.3869172169102093 1103 3 0.4926936820170269 1103 4 0.29532783944525676 1103 5 0.21911228962369478 1103 6 0.2907988711269951 1103 7 0.3402708336278124 1103 8 0.2570552466687772 1103 9 0.4276555099662076 1103 10-11 0.450154363019692 1103 12-13 0.5011237314980974 1103 14-15 0.7319209249909662 1103 16-17 0.7331997902090777 1103 18-19 0.6549686686359522 1103 20-21 0.7112730095850281 1103 22-23 0.6003014145845356 1103 24-25 0.5856516728697443 1103 26-27 0.6889659775191106 1103 28-29 0.701370144690955 1103 30-31 0.6975833235860804 1103 32-33 0.7893934505731508 1103 34-35 0.892434006645864 1103 36-37 0.906468381795726 1103 38-39 0.9973415735088267 1103 40-41 0.9153214430338821 1103 42-43 0.8895308019873127 1103 44-45 1.1524441275087511 1103 46-47 1.1820119975993393 1103 48-49 1.4187732998458316 1103 50-51 1.4506579432492828 1103 52-53 1.5310904257727174 1103 54-55 1.6669560448210419 1103 56-57 1.7322492806555303 1103 58-59 1.9008891117035063 1103 60-61 1.8753354910880589 1103 62-63 2.085354826118646 1103 64-65 2.0177716588258363 1103 66-67 2.006232649861623 1103 68-69 2.1185142775665646 1103 70-71 2.0955968795274345 1103 72-73 2.20196201385183 1103 74-75 2.4697596309487295 1103 76-77 2.299500003880336 1103 78-79 2.585062687861683 1103 80-81 2.9067518593391135 1103 82-83 3.2115024133781276 1103 84-85 3.2078596440713483 1103 86-87 3.3334876010821404 1103 88-89 3.593227776458498 1103 90-91 3.7703123994060164 1103 92-93 3.813749020746261 1103 94-95 3.7520361019689723 1103 96-97 4.010269625787508 1103 98-99 4.4275848366100305 1103 100-101 4.557253278191631 1104 1 0.2974826144517273 1104 2 0.2859553709003997 1104 3 0.21263867530770142 1104 4 0.21654948660607687 1104 5 0.11353406442356828 1104 6 0.15488605250915555 1104 7 0.18935478264143057 1104 8 0.21007339083016063 1104 9 0.25810216384387985 1104 10-11 0.3230285821148229 1104 12-13 0.30763938120416867 1104 14-15 0.3836196682430639 1104 16-17 0.41964828266099374 1104 18-19 0.4985962553774428 1104 20-21 0.5561609942803756 1104 22-23 0.36674500768390317 1104 24-25 0.45423237333416466 1104 26-27 0.4429674917276145 1104 28-29 0.4778432994751185 1104 30-31 0.4951422268234964 1104 32-33 0.5077050441270714 1104 34-35 0.4868350664412944 1104 36-37 0.6663393354084164 1104 38-39 0.6514641227977407 1104 40-41 0.767772662114723 1104 42-43 0.8390498789824008 1104 44-45 0.8832999638718846 1104 46-47 0.8225924655138428 1104 48-49 1.0310180499750814 1104 50-51 0.9843113588355408 1104 52-53 1.2727174059277075 1104 54-55 1.2318603305880131 1104 56-57 1.2944275019422022 1104 58-59 1.452318860933417 1104 60-61 1.4239872472162034 1104 62-63 1.3977869520237292 1104 64-65 1.4730718401244616 1104 66-67 1.7592972851909963 1104 68-69 1.8239989371744478 1104 70-71 1.7140715590563502 1104 72-73 1.975470815753166 1104 74-75 2.0937156022510166 1104 76-77 2.0817370475011323 1104 78-79 2.295931461224857 1104 80-81 2.7172701521367664 1104 82-83 2.76753123225787 1104 84-85 2.8869998415656823 1104 86-87 2.8884394655077976 1104 88-89 2.9769049362187836 1104 90-91 3.2146296772625256 1104 92-93 3.5089128668358462 1104 94-95 3.6916230775181162 1104 96-97 3.951327724131289 1104 98-99 4.129488490955065 1104 100-101 4.368431214999767 1105 1 0.37114270331215593 1105 2 0.35113005332081215 1105 3 0.2830813035676414 1105 4 0.21068913793261856 1105 5 0.13881543757548798 1105 6 0.22805744541997086 1105 7 0.3001033644043076 1105 8 0.23991914048537666 1105 9 0.32907515274906274 1105 10-11 0.37879450294098405 1105 12-13 0.4476776981688033 1105 14-15 0.6040166290855282 1105 16-17 0.4817500573223086 1105 18-19 0.5358333038431269 1105 20-21 0.5831035879713653 1105 22-23 0.4060583771387698 1105 24-25 0.42952757407066855 1105 26-27 0.543509860538407 1105 28-29 0.5865520396002282 1105 30-31 0.6277884262390998 1105 32-33 0.6695692026639719 1105 34-35 0.7108074585770296 1105 36-37 0.6829591823588927 1105 38-39 0.7396774715112286 1105 40-41 0.7235556854797025 1105 42-43 0.976922632454766 1105 44-45 0.9659656310710361 1105 46-47 0.9975517105277305 1105 48-49 1.0230911142042132 1105 50-51 1.020978747205092 1105 52-53 1.2540000417564343 1105 54-55 1.2272363681117113 1105 56-57 1.1697348018152027 1105 58-59 1.375305127958633 1105 60-61 1.4537390238788817 1105 62-63 1.5983220431584257 1105 64-65 1.7752960798802206 1105 66-67 1.7524132404041808 1105 68-69 1.7685984466590732 1105 70-71 1.8173915439187809 1105 72-73 1.9041503178145724 1105 74-75 2.114417042867 1105 76-77 1.9956793464462308 1105 78-79 2.2806812729149897 1105 80-81 2.600612461270611 1105 82-83 2.6077135562381777 1105 84-85 2.7593435807628808 1105 86-87 2.8925954734113546 1105 88-89 3.1435097729623145 1105 90-91 3.292508634953755 1105 92-93 3.360920504756905 1105 94-95 3.535891998566246 1105 96-97 3.96451205311136 1105 98-99 4.672810586343562 1105 100-101 4.757948632298074 1106 1 0.12354666948188608 1106 2 0.13816547488746878 1106 3 0.08376502576873435 1106 4 0.162613445564034 1106 5 0.0416404666010024 1106 6 0.049305846633821204 1106 7 0.09975914313684342 1106 8 0.08310511165321799 1106 9 0.10107104605862816 1106 10-11 0.18553559353780713 1106 12-13 0.06546963493084945 1106 14-15 -0.11101118911263086 1106 16-17 -0.02079665355815763 1106 18-19 0.22610233563911208 1106 20-21 0.39990316531992676 1106 22-23 0.2232926790785541 1106 24-25 0.15964729807637923 1106 26-27 0.11186038526705033 1106 28-29 0.294450247416556 1106 30-31 0.36890298783355746 1106 32-33 0.2561110770177635 1106 34-35 0.3153019425946155 1106 36-37 0.4415545925604434 1106 38-39 0.4620299769645797 1106 40-41 0.4747664964965921 1106 42-43 0.6314065411090368 1106 44-45 0.13713047329841999 1106 46-47 0.004360973638227961 1106 48-49 0.49186447191383564 1106 50-51 0.295363855641142 1106 52-53 0.60936002621564 1106 54-55 0.6173950167164364 1106 56-57 0.5679355376426329 1106 58-59 1.1419617790451682 1106 60-61 0.8675233064380592 1106 62-63 1.0915037932585108 1106 64-65 0.6430422203264925 1106 66-67 0.6841901646891202 1106 68-69 0.7961621098923111 1106 70-71 0.8234533603213947 1106 72-73 1.2474826193313397 1106 74-75 1.6356711629468457 1106 76-77 1.5778713423940083 1106 78-79 1.9111506647272059 1106 80-81 1.8542652280670602 1106 82-83 2.1616882134920097 1106 84-85 2.104904152177447 1106 86-87 2.509711494678271 1106 88-89 2.8781773570682248 1106 90-91 2.6398819961128517 1106 92-93 2.3116492062929197 1106 94-95 2.8998458046531184 1106 96-97 3.516527953374336 1106 98-99 3.805370359840161 1106 100-101 4.043959741422206 1107 1 0.16127953065828393 1107 2 0.10529706795294302 1107 3 0.1720661088315225 1107 4 0.11351578959647668 1107 5 0.042959816138477436 1107 6 0.07916045931368387 1107 7 0.09479114048743043 1107 8 0.17552545748704773 1107 9 0.2506374371020428 1107 10-11 0.27442859857508495 1107 12-13 0.2633915255105279 1107 14-15 0.3005746279691053 1107 16-17 0.42838035976655675 1107 18-19 0.33995131028671466 1107 20-21 0.23259482583469548 1107 22-23 0.2397896364514196 1107 24-25 0.22839621014860967 1107 26-27 0.3157721048936679 1107 28-29 0.2460831433173709 1107 30-31 0.30953672194088 1107 32-33 0.29964264901648363 1107 34-35 0.4033379952936187 1107 36-37 0.4707537259673913 1107 38-39 0.5078414885124545 1107 40-41 0.596935228626684 1107 42-43 0.7641053428932807 1107 44-45 0.751854789053219 1107 46-47 0.8051244646121134 1107 48-49 0.8284165241807884 1107 50-51 0.7532296515490131 1107 52-53 0.8386461938444469 1107 54-55 0.9247279400218815 1107 56-57 0.9370900738216434 1107 58-59 0.9173040467017373 1107 60-61 0.8615451712199089 1107 62-63 0.9464762145684134 1107 64-65 1.1666886596448904 1107 66-67 1.3118225928367906 1107 68-69 1.4892056207546283 1107 70-71 1.5239898481915048 1107 72-73 1.7328308767276859 1107 74-75 1.7395263772786578 1107 76-77 1.778388905899977 1107 78-79 2.035722523305079 1107 80-81 2.3179341088248187 1107 82-83 2.3695952948641015 1107 84-85 2.5230023607007297 1107 86-87 2.6661398423045597 1107 88-89 2.9297233080130596 1107 90-91 2.736868737453392 1107 92-93 2.9085309709720235 1107 94-95 3.1967381919705815 1107 96-97 3.607457291405435 1107 98-99 3.888734951105139 1107 100-101 4.012986805940898 1108 1 0.2201484330054484 1108 2 0.21105327805697627 1108 3 0.2209738794784215 1108 4 0.21653701435683104 1108 5 0.11990613656305271 1108 6 0.06962201499263188 1108 7 0.16999161568790555 1108 8 0.16845598954800778 1108 9 0.15275905226713604 1108 10-11 0.1961858072888134 1108 12-13 0.20372933106573043 1108 14-15 0.34957666392766384 1108 16-17 0.4819290828805052 1108 18-19 0.45829904167792535 1108 20-21 0.44248579659522846 1108 22-23 0.37335841784629054 1108 24-25 0.3886782313001973 1108 26-27 0.3946437620261918 1108 28-29 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2.4344219979098796 2110 92-93 2.504558512160852 2110 94-95 2.5102640006576777 2110 96-97 2.8859038521100615 2110 98-99 3.0147336545231944 2110 100-101 2.995830567816917 2111 1 0.001987760255403259 2111 2 -0.1817386857494796 2111 3 -0.10477312356326962 2111 4 -0.12126172295881332 2111 5 -0.039524377456828574 2111 6 -0.12396232158687326 2111 7 -0.10972619736324418 2111 8 -0.014467099172179587 2111 9 0.02329556531572763 2111 10-11 0.03579038771464127 2111 12-13 -0.09379386500819464 2111 14-15 -0.09917854400628556 2111 16-17 -0.009767617270306062 2111 18-19 0.15590786513215704 2111 20-21 0.0595358694423993 2111 22-23 0.08204128981418535 2111 24-25 -0.01863971466509895 2111 26-27 0.029326685114916984 2111 28-29 -0.08017944405631994 2111 30-31 0.030559026296522518 2111 32-33 0.02120224279830296 2111 34-35 0.13115042310604252 2111 36-37 0.2525526188773881 2111 38-39 0.23320360276346008 2111 40-41 0.30751717809751966 2111 42-43 0.3138448259661075 2111 44-45 0.36384755104598554 2111 46-47 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1.7848470704496329 2316 86-87 1.9897097503056074 2316 88-89 2.3101881579389847 2316 90-91 2.3829727871502584 2316 92-93 2.461270398561748 2316 94-95 3.069424835315907 2316 96-97 2.981198374727356 2316 98-99 3.344530636242144 2316 100-101 3.823768983788945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 40.0 5 88.0 6 199.0 7 467.0 8 1034.0 9 1881.0 10 2852.0 11 4555.0 12 6645.0 13 9328.0 14 12084.0 15 16004.0 16 20283.0 17 25113.0 18 24593.0 19 21874.0 20 22745.0 21 24356.0 22 27296.0 23 30631.0 24 35175.0 25 40988.0 26 48062.0 27 57004.0 28 67415.0 29 79160.0 30 93177.0 31 109675.0 32 128588.0 33 154321.0 34 188728.0 35 233080.0 36 284982.0 37 308188.0 38 156983.0 39 7300.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.060764911261636 19.590371836070336 15.164589823930893 23.184273428737136 2 15.997969666207062 20.735875677260708 39.14435423709629 24.121800419435946 3 19.305879250500688 24.419442722030976 29.958947165857726 26.31573086161061 4 13.417562171591277 17.21387348123493 36.142510454734165 33.22605389243963 5 14.011315104433741 37.51441364875554 33.79986141494097 14.674409831869747 6 32.60135345821403 35.9878053343644 17.09039447355714 14.320446733864431 7 29.029059439195056 32.52950221745491 20.156121654440522 18.285316688909518 8 26.594224460959786 35.37851750355024 19.347628151788307 18.67962988370166 9 26.508386059879303 16.666547879742495 19.760783922427386 37.06428213795082 10-11 25.278028220209574 26.72779293300952 26.967668278011292 21.02651056876961 12-13 25.98101666164792 23.838085701202182 27.5918564436264 22.5890411935235 14-15 22.69131673523642 25.325491020599433 25.788804569970548 26.1943876741936 16-17 22.526299425011896 28.682186772244485 26.31167491344888 22.479838889294747 18-19 22.49803556124149 26.955930645066452 28.803393979997473 21.742639813694588 20-21 23.164831111720886 26.159419773390187 28.817403412867122 21.858345702021815 22-23 22.802679476648567 26.153495275547055 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48-49 22.681360906154172 26.86715226761269 27.717005892425373 22.73448093380777 50-51 22.699468487907787 26.92200955403231 27.598070484597198 22.780451473462705 52-53 23.0357616944242 26.857018258144176 27.440670385885262 22.666549661546355 54-55 22.587036664178083 26.80338596188009 27.6329270226592 22.976650351282633 56-57 22.66254060285552 27.116693898569032 27.48015961399001 22.740605884585435 58-59 22.703677999533166 26.692691396916413 27.55806898788195 23.04556161566847 60-61 22.595255234494296 26.83269663541977 27.735670287886048 22.836377842199887 62-63 22.573405864629233 26.895215678448547 27.658273182604603 22.873105274317616 64-65 22.311235698796747 26.695965461513925 27.6307665854758 23.36203225421352 66-67 22.337812055800875 26.970668324935822 27.481067178164302 23.210452441099 68-69 22.54933491795443 26.689075869822904 27.595281060140024 23.166308152082642 70-71 22.284709093792372 26.83136028252282 27.43360998807973 23.450320635605074 72-73 22.519149937013232 26.797305556198985 27.3082823588945 23.375262147893284 74-75 22.435316065278165 26.93684307118841 27.141193701679704 23.486647161853718 76-77 22.367718881240993 26.79423194453601 27.462675663588303 23.3753735106347 78-79 22.459325872326627 26.868577710702763 27.31581048021396 23.356285936756656 80-81 22.3150319722573 27.153322063418884 27.209471182650567 23.32217478167325 82-83 22.51965013953882 26.975582376904043 27.15882034571773 23.34594713783941 84-85 22.465391983631648 26.739076348900216 27.16020152177046 23.635330145697683 86-87 22.532190944217213 26.801561415590815 27.17864069712226 23.487606943069714 88-89 22.33311231536332 26.85991967180947 27.041663706273134 23.76530430655408 90-91 22.29419536584323 26.84113206135162 27.25522696819808 23.609445604607075 92-93 22.444660983445903 26.608230833050285 27.148675059718364 23.798433123785447 94-95 22.18570761667697 26.56355516197488 27.139723713493062 24.111013507855084 96-97 22.378320614223426 26.451702424500517 27.120457959228766 24.049519002047294 98-99 22.152566064832715 26.45208105782132 26.9653742055382 24.429978671807763 100-101 22.183027224808576 26.276303498461623 27.2624494354094 24.278219841320396 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 577.0 1 391.0 2 284.0 3 375.0 4 332.5 5 325.0 6 590.0 7 1246.0 8 1882.5 9 2129.0 10 1903.0 11 1630.0 12 1672.5 13 1783.5 14 1949.0 15 2114.0 16 2210.5 17 2339.5 18 2566.0 19 2824.5 20 3205.5 21 3691.0 22 4358.5 23 5174.5 24 6246.0 25 7874.5 26 10049.0 27 12939.5 28 15620.5 29 18547.5 30 22131.5 31 26113.0 32 31106.5 33 35215.0 34 38515.5 35 42746.0 36 47897.0 37 52970.0 38 57150.0 39 60090.0 40 63363.5 41 68765.5 42 73738.5 43 78495.5 44 82304.5 45 85150.0 46 86445.0 47 87631.5 48 91548.0 49 93036.0 50 91198.5 51 87700.5 52 83584.5 53 81951.5 54 80560.0 55 74264.0 56 65907.5 57 59021.0 58 53084.5 59 46312.0 60 38471.0 61 31268.5 62 25621.0 63 19999.0 64 15144.0 65 11986.5 66 8993.0 67 6915.0 68 5166.0 69 3596.5 70 2781.0 71 2201.0 72 1876.0 73 1687.0 74 1392.0 75 1156.0 76 826.5 77 488.5 78 299.5 79 197.0 80 133.5 81 83.5 82 46.0 83 25.5 84 11.5 85 6.0 86 2.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.909019312972066E-5 2 0.04231784173661732 3 1.7818038625944132E-4 4 0.0 5 0.10022646727093575 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013808979935106703 26-27 0.01198263097594743 28-29 0.0 30-31 0.0 32-33 4.2317841736617316E-4 34-35 0.014454883835297177 36-37 0.0027617959870213406 38-39 0.011448089817169107 40-41 0.006481311550187179 42-43 0.0049445057186994976 44-45 0.0024722528593497488 46-47 4.454509656486033E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2272548282430165E-4 68-69 2.2272548282430165E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.454509656486033E-5 82-83 9.354470278620669E-4 84-85 0.004298601818509022 86-87 0.00133635289694581 88-89 2.2272548282430165E-5 90-91 8.909019312972066E-4 92-93 1.5590783797701116E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.68176448472905E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2244916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.4659927692814 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48733306096088 37.82098551949186 2 14.485581438581505 14.91029659153745 3 5.119919418243416 7.905052072758571 4 2.3003075612630894 4.73550449260358 5 1.275535926938434 3.2823361396386046 6 0.7372752670417643 2.276676213752487 7 0.4965178109548195 1.7887647447894117 8 0.34562196055569144 1.423022185829126 9 0.2554970156170912 1.1834466802489974 >10 1.321812247774901 12.426760504917416 >50 0.10618597327830337 3.782678827341779 >100 0.06285285780621803 5.757840169056599 >500 0.004517062049575118 1.5188223052590344 >1k 8.686657787644045E-4 0.6407370935440398 >5k 1.7373315575288091E-4 0.5470764592309807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6666 0.29693761370135896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5571 0.24816073296283692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.17639858239684691 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.454509656486033E-5 0.0 8 0.0 0.0 0.0 4.454509656486033E-5 0.0 9 0.0 0.0 0.0 4.454509656486033E-5 0.0 10-11 0.0 0.0 0.0 8.909019312972066E-5 0.0 12-13 0.0 0.0 0.0 1.1136274141215083E-4 0.0 14-15 0.0 0.0 0.0 1.33635289694581E-4 0.0 16-17 0.0 0.0 0.0 1.33635289694581E-4 0.0 18-19 0.0 0.0 0.0 2.2272548282430168E-4 0.0 20-21 0.0 0.0 0.0 3.340882242364525E-4 0.0 22-23 0.0 0.0 0.0 4.899960622134637E-4 0.0 24-25 0.0 0.0 0.0 9.577195761444972E-4 0.0 26-27 0.0 0.0 0.0 0.0017149862177471228 0.0 28-29 0.0 0.0 0.0 0.005144958653241369 0.0 30-31 0.0 0.0 0.0 0.015590783797701117 0.0 32-33 0.0 0.0 0.0 0.036482434086620615 0.0 34-35 0.0 0.0 0.0 0.06830990558221332 0.0 36-37 0.0 0.0 0.0 0.11975949211462701 0.0 38-39 0.0 0.0 0.0 0.19664432878557594 0.0 40-41 0.0 0.0 0.0 0.27934230055823916 0.0 42-43 0.0 0.0 0.0 0.3656929702492209 0.0 44-45 0.0 0.0 0.0 0.4721780235875196 0.0 46-47 0.0 0.0 0.0 0.5984856448971809 0.0 48-49 0.0 0.0 0.0 0.7354172717375618 0.0 50-51 0.0 0.0 0.0 0.8787856650315646 0.0 52-53 0.0 0.0 0.0 1.0237354092536202 0.0 54-55 0.0 0.0 0.0 1.1839195765008579 0.0 56-57 0.0 0.0 0.0 1.367267193961823 0.0 58-59 0.0 0.0 0.0 1.5655819638685813 0.0 60-61 0.0 0.0 0.0 1.7838306644881143 4.454509656486033E-5 62-63 0.0 0.0 0.0 1.998114851513375 4.454509656486033E-5 64-65 0.0 0.0 0.0 2.2281902752708787 4.454509656486033E-5 66-67 0.0 0.0 0.0 2.485549570674359 4.454509656486033E-5 68-69 0.0 0.0 0.0 2.7712618200413734 4.454509656486033E-5 70-71 0.0 0.0 0.0 3.0786675314354746 4.454509656486033E-5 72-73 0.0 0.0 0.0 3.396318615039494 4.454509656486033E-5 74-75 0.0 0.0 0.0 3.725885511974613 4.454509656486033E-5 76-77 0.0 0.0 0.0 4.070419561355525 4.454509656486033E-5 78-79 0.0 0.0 0.0 4.45246058204405 4.454509656486033E-5 80-81 0.0 0.0 0.0 4.8725653877472475 4.454509656486033E-5 82-83 0.0 0.0 0.0 5.305098275392041 4.454509656486033E-5 84-85 0.0 0.0 0.0 5.731461667162602 4.454509656486033E-5 86-87 0.0 0.0 0.0 6.17475219562781 4.454509656486033E-5 88-89 0.0 0.0 0.0 6.65537151501437 4.454509656486033E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3935 0.0 55.35955 1 GTATCAA 7120 0.0 41.484264 1 ATCAACG 9335 0.0 31.628923 3 TCAACGC 9565 0.0 30.868374 4 TATCAAC 9610 0.0 30.834467 2 CAACGCA 9770 0.0 30.220676 5 AACGCAG 10075 0.0 29.415901 6 ACGCAGA 11770 0.0 24.937597 7 TGGTATC 1895 0.0 24.848362 2 CGCAGAG 11830 0.0 24.811117 8 GTACATG 9455 0.0 24.24696 1 GTGGTAT 2175 0.0 23.398947 1 TACATGG 9680 0.0 23.142876 2 ACATGGG 9775 0.0 22.03376 3 GCAGAGT 13050 0.0 22.018486 9 GAGTACT 6890 0.0 21.506908 12-13 AGTACTT 7345 0.0 20.433271 12-13 AGAGTAC 12300 0.0 20.078941 10-11 GTACTTT 7355 0.0 20.05033 14-15 CATGGGG 4540 0.0 19.89779 4 >>END_MODULE