FastQCFastQC Report
Thu 26 May 2016
SRR938368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251764
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66430.2950131541316053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.2269331954858502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.15934174274035823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.057.046961
GTATCAA70500.046.9564061
ATCAACG92900.035.0966953
TATCAAC96250.034.072672
TCAACGC95200.034.0490724
CAACGCA97150.033.414565
AACGCAG100650.032.2933656
ACGCAGA115450.027.9003837
CGCAGAG117850.027.2918858
GTACATG100000.026.3784711
TACATGG100300.025.6836172
GTGGTAT17900.025.0495151
TGGTATC16350.024.7092172
GCAGAGT127850.024.4872429
ACATGGG104250.023.9810123
GTAAGGT8350.021.6299214
AGAGTAC120200.021.33985110-11
GAGTACT66550.020.8766412-13
TAAGGTG10950.020.8345895
CATGGGG51350.020.6405964
Produced by FastQC (version 0.11.5)