##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2251764 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23159354177436 31.0 31.0 34.0 28.0 34.0 2 31.407875780943296 31.0 31.0 34.0 28.0 34.0 3 31.460584679389136 31.0 31.0 34.0 28.0 34.0 4 35.14322015983913 37.0 35.0 37.0 32.0 37.0 5 35.133458479663055 37.0 35.0 37.0 32.0 37.0 6 35.11639718904823 37.0 35.0 37.0 32.0 37.0 7 35.036117461687816 37.0 35.0 37.0 32.0 37.0 8 34.99363299173448 37.0 35.0 37.0 32.0 37.0 9 36.494706816522516 39.0 37.0 39.0 32.0 39.0 10-11 36.33059881053254 39.0 35.0 39.0 32.0 39.0 12-13 36.14370244839157 38.0 35.0 39.0 31.5 39.0 14-15 37.33301114148729 39.0 37.0 41.0 32.0 41.0 16-17 37.270890732776614 39.0 37.0 41.0 32.0 41.0 18-19 37.25867653093309 39.5 37.0 41.0 31.5 41.0 20-21 37.19507417295951 39.5 36.5 41.0 31.0 41.0 22-23 37.04327784794499 39.0 36.0 41.0 31.0 41.0 24-25 36.905304463522825 39.0 36.0 41.0 30.5 41.0 26-27 36.7017045303149 39.0 36.0 41.0 30.0 41.0 28-29 36.465912502375915 39.0 36.0 40.5 30.0 41.0 30-31 36.194823258565286 39.0 35.0 40.0 29.5 41.0 32-33 35.915428526257635 38.0 35.0 40.0 28.5 41.0 34-35 35.61224733142549 38.0 34.5 40.0 27.0 41.0 36-37 35.39430375474517 38.0 34.0 40.0 26.5 41.0 38-39 35.16044132511222 38.0 34.0 40.0 25.5 41.0 40-41 34.87969520784594 38.0 34.0 40.0 25.0 41.0 42-43 34.469427746424586 38.0 33.0 40.0 24.0 41.0 44-45 33.99291599830178 38.0 33.0 40.0 23.5 41.0 46-47 33.49756613037601 37.5 32.5 40.0 20.5 41.0 48-49 33.02002896395892 37.0 31.5 40.0 18.5 41.0 50-51 32.30061898138526 36.5 30.5 39.0 15.0 40.5 52-53 32.30801917962984 36.5 31.0 39.0 14.5 40.0 54-55 32.27183710193431 37.0 31.0 39.0 11.0 40.0 56-57 31.848624900300386 36.0 30.5 39.0 8.0 40.0 58-59 31.387666735945686 36.0 30.0 39.0 6.5 40.0 60-61 30.836916968208037 35.0 29.0 39.0 2.0 40.0 62-63 30.09605313878364 35.0 28.0 38.0 2.0 40.0 64-65 29.414325613163726 34.0 26.5 38.0 2.0 40.0 66-67 28.707125391470864 34.0 26.0 37.5 2.0 39.5 68-69 27.845141409135238 33.5 25.0 37.0 2.0 39.0 70-71 26.99864306383795 32.5 22.5 36.0 2.0 39.0 72-73 26.061349679628947 32.0 20.0 35.5 2.0 38.5 74-75 25.08501668025601 31.0 15.5 35.0 2.0 37.5 76-77 24.103858574877293 30.5 7.5 34.5 2.0 37.0 78-79 23.086158007677536 29.5 2.0 34.0 2.0 36.0 80-81 22.067068307335937 29.0 2.0 34.0 2.0 35.5 82-83 21.01953290842202 27.5 2.0 34.0 2.0 35.0 84-85 19.999134012267717 26.0 2.0 33.0 2.0 35.0 86-87 18.962489408303888 25.5 2.0 32.5 2.0 35.0 88-89 17.830091652588813 22.5 2.0 32.0 2.0 34.0 90-91 16.633122076736285 18.5 2.0 31.0 2.0 34.0 92-93 15.42318822043518 4.5 2.0 31.0 2.0 34.0 94-95 14.118871249384927 2.0 2.0 30.5 2.0 34.0 96-97 12.6747640960598 2.0 2.0 29.0 2.0 34.0 98-99 10.955094983310861 2.0 2.0 27.5 2.0 33.5 100-101 7.700157965044294 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5098710401302782 1101 2 0.394833170902519 1101 3 0.42605994260406277 1101 4 0.3068314843589732 1101 5 0.2841165731398192 1101 6 0.37102763690053564 1101 7 0.28354350782650783 1101 8 0.26345988289925515 1101 9 0.32642668214307236 1101 10-11 0.421887822922244 1101 12-13 0.3685861796941907 1101 14-15 0.4482008633791139 1101 16-17 0.5307029034802753 1101 18-19 0.4533646982764523 1101 20-21 0.5945966218574625 1101 22-23 0.595450385017763 1101 24-25 0.5075662281129851 1101 26-27 0.645905235545321 1101 28-29 0.5893616544787292 1101 30-31 0.6108499907493581 1101 32-33 0.649142292087113 1101 34-35 0.7953808128749884 1101 36-37 0.7812979766770596 1101 38-39 0.8560214352711739 1101 40-41 0.8222482041725385 1101 42-43 0.8498212129947262 1101 44-45 0.8805850603015699 1101 46-47 0.9704862276893635 1101 48-49 0.9245155085944958 1101 50-51 0.9108400815259472 1101 52-53 0.9470331497505668 1101 54-55 1.1291785472152043 1101 56-57 1.2062449556159542 1101 58-59 1.0633724317245878 1101 60-61 1.0445989486237295 1101 62-63 1.095623360091782 1101 64-65 1.067932616357183 1101 66-67 1.381486291475948 1101 68-69 1.383508972898774 1101 70-71 1.5625498499360688 1101 72-73 1.8879621954345644 1101 74-75 1.8699457765233802 1101 76-77 2.0558415422459966 1101 78-79 2.3459111674269018 1101 80-81 2.721691316450329 1101 82-83 2.810570142841595 1101 84-85 3.101453262737074 1101 86-87 3.2910423417250527 1101 88-89 3.3629019060194985 1101 90-91 3.439909028106033 1101 92-93 3.38406245672315 1101 94-95 3.146211465451552 1101 96-97 3.2072036680733884 1101 98-99 3.254279532173811 1101 100-101 2.3495105093181863 1102 1 0.5032877404575551 1102 2 0.40795762822457604 1102 3 0.3047060227597811 1102 4 0.24843814502504102 1102 5 0.36963426801884225 1102 6 0.3003236583754756 1102 7 0.3662054842715037 1102 8 0.3326729879553483 1102 9 0.2823218741357678 1102 10-11 0.38538567447418615 1102 12-13 0.392083737047102 1102 14-15 0.5155424252738214 1102 16-17 0.5974107447591024 1102 18-19 0.6209527910079871 1102 20-21 0.5317622686341537 1102 22-23 0.4135715743451769 1102 24-25 0.5199285509177045 1102 26-27 0.4748199512218392 1102 28-29 0.5066084365774302 1102 30-31 0.352601882369612 1102 32-33 0.3401742707266777 1102 34-35 0.4108920989859328 1102 36-37 0.5279671665642525 1102 38-39 0.6253270539955906 1102 40-41 0.7576460725812879 1102 42-43 0.954760019596236 1102 44-45 0.826616434640421 1102 46-47 0.8164990754405679 1102 48-49 0.9225356286276991 1102 50-51 1.1184571329837425 1102 52-53 1.1249130402131087 1102 54-55 1.2489455813391075 1102 56-57 1.3570424337736604 1102 58-59 1.5697472078947854 1102 60-61 1.5039851699313793 1102 62-63 1.5845219186755557 1102 64-65 1.7523343825055306 1102 66-67 1.8639675342998743 1102 68-69 2.048944045557988 1102 70-71 2.01587159553819 1102 72-73 2.232905391167563 1102 74-75 2.1797983828087446 1102 76-77 2.348515111723085 1102 78-79 2.915902707216919 1102 80-81 3.183501300452267 1102 82-83 3.338554036637337 1102 84-85 3.393467071008363 1102 86-87 3.255988328373185 1102 88-89 3.4303104578287424 1102 90-91 3.376829575284429 1102 92-93 3.5726796179092304 1102 94-95 3.3765492297933566 1102 96-97 3.310978997019708 1102 98-99 2.920185124499829 1102 100-101 1.933399375236581 1103 1 0.3418034490574442 1103 2 0.39489651056981856 1103 3 0.34507223599026204 1103 4 0.19195666354355723 1103 5 0.1261715629525213 1103 6 0.2717290795938254 1103 7 0.3382972184916895 1103 8 0.25042936416110706 1103 9 0.38357690696947344 1103 10-11 0.41202909195110493 1103 12-13 0.4197168809996228 1103 14-15 0.5228473476353841 1103 16-17 0.5616650192996957 1103 18-19 0.5513844022076242 1103 20-21 0.6061718970320342 1103 22-23 0.6382974148187799 1103 24-25 0.6820464212303321 1103 26-27 0.7847203915707297 1103 28-29 0.7553354087168955 1103 30-31 0.7474390418113188 1103 32-33 0.9443569115882795 1103 34-35 0.8702618303885004 1103 36-37 0.9140919423481364 1103 38-39 1.1160917560287444 1103 40-41 1.1218184553152994 1103 42-43 1.1909608171512218 1103 44-45 1.2573668561282076 1103 46-47 1.5044704104213622 1103 48-49 1.3414444563912369 1103 50-51 1.4617248758877395 1103 52-53 1.692889205944418 1103 54-55 1.7658642746114737 1103 56-57 1.8196810051032521 1103 58-59 1.8368818414890953 1103 60-61 2.036430876914597 1103 62-63 2.143919268113958 1103 64-65 2.243671268653788 1103 66-67 2.3117605934159435 1103 68-69 2.4662020461276697 1103 70-71 2.5822395640055156 1103 72-73 2.9373029138201296 1103 74-75 2.863886641198082 1103 76-77 3.0818270819764386 1103 78-79 3.4752154704062157 1103 80-81 3.314857201666303 1103 82-83 3.6127016305001654 1103 84-85 3.8926214972075854 1103 86-87 4.0582334726028435 1103 88-89 4.24727524531421 1103 90-91 4.09697130296097 1103 92-93 4.20505151641343 1103 94-95 4.047605624475297 1103 96-97 4.0575061493019025 1103 98-99 3.981741396007253 1103 100-101 2.6923726386683757 1104 1 0.4851585688831541 1104 2 0.38762886556632736 1104 3 0.4425770660145183 1104 4 0.30698112196424177 1104 5 0.3115376171310089 1104 6 0.43479891968919304 1104 7 0.3724814582253231 1104 8 0.40099457976516817 1104 9 0.500689580130981 1104 10-11 0.521948111970012 1104 12-13 0.5255129101030818 1104 14-15 0.6751020440632232 1104 16-17 0.6432698205493352 1104 18-19 0.6563582995233688 1104 20-21 0.5645638072552472 1104 22-23 0.6011247260367654 1104 24-25 0.6184586381086987 1104 26-27 0.7028689507847687 1104 28-29 0.729557466512766 1104 30-31 0.7841472261332711 1104 32-33 0.7362383601844869 1104 34-35 0.8037801433740768 1104 36-37 0.9822096716201401 1104 38-39 0.9639069408028647 1104 40-41 0.8762887969110551 1104 42-43 0.9734288567779714 1104 44-45 0.8920690388881027 1104 46-47 1.1525199027549817 1104 48-49 1.1480091787162294 1104 50-51 0.9026423749628378 1104 52-53 1.125198985202033 1104 54-55 1.5880371689264265 1104 56-57 1.5291519632978456 1104 58-59 1.448362911904681 1104 60-61 1.5386711860361508 1104 62-63 1.67474098883579 1104 64-65 1.5883294673630388 1104 66-67 1.9554856725461285 1104 68-69 2.1637808680128146 1104 70-71 2.145276518829373 1104 72-73 2.4784341671600387 1104 74-75 2.5762802778980465 1104 76-77 2.754203453908861 1104 78-79 2.8569435914240167 1104 80-81 3.005086165369189 1104 82-83 3.2782296446408346 1104 84-85 3.440740870743163 1104 86-87 3.608621929872541 1104 88-89 3.732542643678169 1104 90-91 3.6863616922403715 1104 92-93 3.6198293352977977 1104 94-95 3.632564723418861 1104 96-97 3.585359974270043 1104 98-99 3.143277960318512 1104 100-101 2.2236013146251317 1105 1 0.4535614921247877 1105 2 0.3685376842109953 1105 3 0.36474597710612855 1105 4 0.35605230505855445 1105 5 0.28060539252867045 1105 6 0.3861673997247479 1105 7 0.3893268972253523 1105 8 0.34872578276113586 1105 9 0.32349620476353635 1105 10-11 0.3649799431941787 1105 12-13 0.5008776346461374 1105 14-15 0.5391659506712543 1105 16-17 0.5032314927447246 1105 18-19 0.48908105793084644 1105 20-21 0.5540904975123127 1105 22-23 0.5444584194617548 1105 24-25 0.5828357366306633 1105 26-27 0.3933605110267848 1105 28-29 0.5385677502035051 1105 30-31 0.6287429090734449 1105 32-33 0.6171476179653226 1105 34-35 0.6930546816443552 1105 36-37 0.8633420105735325 1105 38-39 0.837930455666303 1105 40-41 0.9542202723470936 1105 42-43 0.9980042340618098 1105 44-45 1.0342246178057053 1105 46-47 1.2024875384195113 1105 48-49 1.355494781637006 1105 50-51 1.0577362227529719 1105 52-53 1.1319964617499707 1105 54-55 1.2310515225311676 1105 56-57 1.452004785055113 1105 58-59 1.606486535761718 1105 60-61 1.8171997110041183 1105 62-63 1.7792863116403943 1105 64-65 1.957653961763615 1105 66-67 1.9882869485001713 1105 68-69 2.162998327364175 1105 70-71 2.2463766427535994 1105 72-73 2.280081015611511 1105 74-75 2.253177763316529 1105 76-77 2.482459319231065 1105 78-79 2.531091206168103 1105 80-81 2.707823915885392 1105 82-83 3.1554757163482527 1105 84-85 2.9316214178014306 1105 86-87 2.959696188676567 1105 88-89 3.172636566121451 1105 90-91 2.9098607467802076 1105 92-93 3.1489678643145993 1105 94-95 3.30521734299783 1105 96-97 3.1696072817066394 1105 98-99 2.6641803148788092 1105 100-101 1.8158712006868623 1106 1 0.2569948261930932 1106 2 0.20398812850354275 1106 3 0.12843561174992502 1106 4 0.1791223555513568 1106 5 0.23624334371161382 1106 6 -0.05849910524735691 1106 7 0.2520392205556732 1106 8 -0.23191990771137938 1106 9 0.10229081592486722 1106 10-11 0.19622815169196173 1106 12-13 0.26825902066250507 1106 14-15 0.3487430018034843 1106 16-17 -0.039948098462033954 1106 18-19 0.3462336167472415 1106 20-21 0.43108968168976247 1106 22-23 0.2610457234989738 1106 24-25 0.4652625661678442 1106 26-27 0.3582303101580422 1106 28-29 0.3265942093131642 1106 30-31 0.07730534154758573 1106 32-33 0.3795427847679633 1106 34-35 0.22188893737164506 1106 36-37 0.3130246335532689 1106 38-39 0.32560350926804915 1106 40-41 0.5455047298251046 1106 42-43 0.09898375030108753 1106 44-45 0.597489319148508 1106 46-47 0.8702614894260634 1106 48-49 0.5506025804595751 1106 50-51 0.35714971248904703 1106 52-53 0.5394609293706267 1106 54-55 0.5169313540748348 1106 56-57 1.0336789411197636 1106 58-59 1.3335395642273085 1106 60-61 1.378781524776084 1106 62-63 1.3853732298155954 1106 64-65 1.4241661660279412 1106 66-67 1.5780190500149587 1106 68-69 1.6567659722829262 1106 70-71 1.7723796855512077 1106 72-73 1.7411056887312597 1106 74-75 1.9570080722266354 1106 76-77 1.9959997685949453 1106 78-79 2.34959547269953 1106 80-81 2.1359598275119644 1106 82-83 2.595100437976374 1106 84-85 2.5032001005787556 1106 86-87 2.0896445493142295 1106 88-89 2.0636917113158155 1106 90-91 2.28237802237388 1106 92-93 2.4683479273754756 1106 94-95 2.428518822503049 1106 96-97 1.93483718932025 1106 98-99 1.9517118122439232 1106 100-101 1.4049734871949209 1107 1 0.20727221347236835 1107 2 0.12714105152771538 1107 3 0.12527668838287553 1107 4 0.007802965871945844 1107 5 0.08936729365160545 1107 6 0.08281925580184435 1107 7 0.09844124352841277 1107 8 0.23846222841068254 1107 9 0.31437002045084483 1107 10-11 0.3107318376168777 1107 12-13 0.23935399751832165 1107 14-15 0.33663679573457017 1107 16-17 0.3988122306996331 1107 18-19 0.26606003539973955 1107 20-21 0.34337384078457234 1107 22-23 0.31720053234518986 1107 24-25 0.2967439651049304 1107 26-27 0.4246601061463835 1107 28-29 0.34053789180929783 1107 30-31 0.46951826800052743 1107 32-33 0.5149147203071323 1107 34-35 0.5462640445206262 1107 36-37 0.5108467662465728 1107 38-39 0.77994587168876 1107 40-41 0.7364460979032756 1107 42-43 0.7351269870574697 1107 44-45 0.6874886333950414 1107 46-47 0.656564078145415 1107 48-49 0.6454720301424217 1107 50-51 0.7567425463652668 1107 52-53 0.8548439226845304 1107 54-55 0.9631681961800993 1107 56-57 1.0494751866194996 1107 58-59 1.0237532621529084 1107 60-61 1.2100227385597258 1107 62-63 1.4249502056427836 1107 64-65 1.5357047396607229 1107 66-67 1.7768541263050324 1107 68-69 1.7235075289433048 1107 70-71 1.8838037383542279 1107 72-73 1.8628403021377409 1107 74-75 2.081482870786555 1107 76-77 2.220534742022 1107 78-79 2.438481723342427 1107 80-81 2.8028031328436995 1107 82-83 2.80012143917644 1107 84-85 2.781836651238791 1107 86-87 2.49645013820502 1107 88-89 2.7720983631244884 1107 90-91 2.63769501191614 1107 92-93 2.707738635415936 1107 94-95 2.656821239784035 1107 96-97 2.3065490871429812 1107 98-99 2.060922167121346 1107 100-101 1.4649741729483932 1108 1 0.3188055222881694 1108 2 0.28052972442884183 1108 3 0.15439826030679882 1108 4 0.11013169341213569 1108 5 0.13344368327018685 1108 6 0.19953145502428526 1108 7 0.13719032412103616 1108 8 0.3173789038336068 1108 9 0.319992853276311 1108 10-11 0.33809996833033296 1108 12-13 0.24448100708016085 1108 14-15 0.3355761936540844 1108 16-17 0.4218401018775353 1108 18-19 0.40189787820734324 1108 20-21 0.3580154032517342 1108 22-23 0.46303299005246146 1108 24-25 0.4357918530948339 1108 26-27 0.5140219060833431 1108 28-29 0.3672020191603593 1108 30-31 0.4978044926511558 1108 32-33 0.448413328061676 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0.5076835555384704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6723.0 3 2276.0 4 2492.0 5 2785.0 6 3490.0 7 4404.0 8 5756.0 9 7466.0 10 9837.0 11 12539.0 12 15695.0 13 19346.0 14 22900.0 15 26184.0 16 31003.0 17 36215.0 18 36052.0 19 36103.0 20 39799.0 21 44338.0 22 49926.0 23 56109.0 24 62437.0 25 70519.0 26 77584.0 27 86517.0 28 95294.0 29 106610.0 30 117707.0 31 130806.0 32 146318.0 33 163386.0 34 181959.0 35 195000.0 36 187863.0 37 128020.0 38 29940.0 39 366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42136821560626 18.701624429479217 14.557586154322138 23.319421200592387 2 15.410862603344794 19.928203383174573 40.516045402186776 24.14488861129386 3 19.21768072829759 24.307936034062095 29.392904678384397 27.081478559255924 4 12.812671608396082 16.40416320858162 36.66026291517014 34.12290226785216 5 14.131310985716711 37.29048553940281 33.694600150556234 14.883603324324252 6 32.558620046122876 35.47818050130859 17.52860462739557 14.434594825172962 7 29.22104174459922 32.32702971906963 20.236147849861254 18.215780686469902 8 26.65448461293768 35.008698928228064 19.566102080456645 18.77071437837761 9 26.799687949538807 16.803677432940695 19.90832184913 36.48831276839049 10-11 25.292272889026712 26.51698681367795 27.073832474489013 21.116907822806326 12-13 26.16428741190242 23.764146144807658 27.52282818500659 22.548738258283336 14-15 22.888030400927896 25.137515156374697 25.899781686013 26.074672756684407 16-17 22.6441272894979 28.486398882744346 26.375242339689336 22.494231488068415 18-19 22.529395380697334 26.874034646769008 28.841100803644764 21.755469168888894 20-21 23.29238855358728 26.076691329493634 28.737491162098276 21.893428954820802 22-23 22.939294285939585 26.06484057517107 28.522434092668426 22.47343104622093 24-25 22.65571789397921 26.204835201940657 28.755123212568062 22.384323691512073 26-27 22.343662049420924 26.67286860552781 28.476739841548458 22.506729503502807 28-29 22.328169372294973 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27.43971578601874 22.741049138302046 54-55 22.563090393329812 26.735021902484974 27.70310152333637 22.99878618084884 56-57 22.65264137803613 27.043568169866827 27.510690403774817 22.793100048322223 58-59 22.74244327879162 26.483753621199547 27.66523173234456 23.108571367664283 60-61 22.64359517965483 26.66701160939544 27.793819588586956 22.895573622362768 62-63 22.680566449537153 26.702710817420222 27.772154361229724 22.844568371812898 64-65 22.39707157922694 26.562107915787475 27.743495815621767 23.297324689363823 66-67 22.40796793675107 26.773252528285063 27.590844058499574 23.227935476464292 68-69 22.593280578431187 26.506884525833208 27.727989891666287 23.171845004069315 70-71 22.415951508012597 26.593863837700905 27.540620411930494 23.449564242356004 72-73 22.671679964965243 26.526280397525447 27.47992041673949 23.322119220769817 74-75 22.556239698438226 26.713018420336144 27.334232297969468 23.39650958325616 76-77 22.694451957543514 26.548693986515502 27.404121382664858 23.352732673276122 78-79 22.738263111494057 26.639478049614603 27.230358574407465 23.39190026448388 80-81 22.621069018278884 26.89929624850933 27.099939818032826 23.37969491517896 82-83 22.870899040842477 26.710260698081907 26.988836108205582 23.430004152870033 84-85 22.87115402039109 26.438550488281077 27.071508261783322 23.618787229544512 86-87 22.917124652541382 26.436965377822247 27.050966222514738 23.594943747121626 88-89 22.858758394679818 26.393590694300734 27.005334351388488 23.74231655963096 90-91 22.818419131390044 26.509224069402798 26.974164520910104 23.698192278297057 92-93 23.07509352129754 26.180211570769167 26.932534751947873 23.812160155985417 94-95 22.844948399938346 26.102128721865164 26.895774227464926 24.157148650731568 96-97 23.07691111798617 25.931758786670862 26.793041422631035 24.198288672711932 98-99 22.94407797943162 25.923337488404773 26.4530369219515 24.679547610212104 100-101 23.879611481258824 25.075679694364304 26.682583710101415 24.36212511427545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 427.0 1 333.5 2 281.5 3 337.0 4 312.0 5 304.5 6 503.5 7 1044.5 8 1697.0 9 1940.0 10 1706.5 11 1450.5 12 1428.0 13 1567.5 14 1795.0 15 1990.0 16 2156.0 17 2336.0 18 2505.5 19 2771.5 20 3159.0 21 3707.5 22 4393.5 23 5245.5 24 6424.5 25 7868.5 26 10045.0 27 12884.0 28 15693.0 29 18774.0 30 22376.0 31 26390.0 32 30918.0 33 35071.5 34 38718.0 35 42912.5 36 47359.5 37 51962.5 38 56635.0 39 60051.5 40 63692.0 41 68397.5 42 72699.5 43 77124.0 44 80897.5 45 83819.0 46 85357.0 47 86716.0 48 90116.0 49 91507.0 50 89801.5 51 87047.0 52 83505.0 53 81207.5 54 79206.5 55 73714.0 56 66464.0 57 60189.0 58 54448.5 59 47831.5 60 40449.0 61 33770.5 62 27802.0 63 22172.0 64 17454.5 65 13888.0 66 10694.5 67 8125.5 68 6105.0 69 4462.5 70 3454.5 71 2744.5 72 2243.5 73 1904.0 74 1533.5 75 1247.0 76 949.5 77 617.5 78 406.0 79 276.0 80 165.0 81 105.0 82 71.5 83 41.5 84 26.0 85 19.0 86 8.5 87 7.0 88 6.5 89 3.0 90 2.0 91 2.5 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3836103605884098 2 0.030331775443607767 3 0.036238255874061404 4 0.044409627296643876 5 0.06403868256176047 6 0.055689672629991416 7 0.03934692978482647 8 0.04050158009453921 9 0.03757054469296072 10-11 0.038680785375376815 12-13 0.03148642575332051 14-15 0.028799643301873557 16-17 0.023692536162759507 18-19 0.0286442096063353 20-21 0.035771954787446644 22-23 0.03470612373232719 24-25 0.04347702512341435 26-27 0.03797023133863051 28-29 0.03315178677694466 30-31 0.03590518366933657 32-33 0.03070925727562924 34-35 0.03190831721263862 36-37 0.03432864190030572 38-39 0.017230935391097824 40-41 0.014855020330727378 42-43 0.015432345485583747 44-45 0.01156870791077573 46-47 0.020139765979027998 48-49 0.017208730577449503 50-51 0.011746346419962304 52-53 0.009814527632558297 54-55 0.008571058068252268 56-57 0.009414840986888502 58-59 0.00413009533858788 60-61 0.008104756981637507 62-63 0.01181296086090727 64-65 0.0077716847769126775 66-67 0.0039746616430496266 68-69 0.006950106671924767 70-71 0.015121478094507241 72-73 0.012678948593191828 74-75 0.012656743779543502 76-77 0.006261757448826787 78-79 0.00928161210499857 80-81 0.011502093469830764 82-83 0.013722574834662958 84-85 0.008526648440955624 86-87 0.0025313487559087008 88-89 0.0183189712598656 90-91 0.0273341256010843 92-93 0.012057213811038813 94-95 0.022515681039398446 96-97 0.02089472964307094 98-99 0.013256273748048199 100-101 0.12487987195816258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2251764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.023100123513565 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61109646461966 40.84745834756791 2 13.399818286932167 14.47799449903649 3 4.672543294724049 7.572758227269878 4 2.124551184970348 4.5909936553273 5 1.1501570412376187 3.1067524498272 6 0.7015079655278599 2.273858103549233 7 0.45364193633894956 1.7155000622944547 8 0.31129341276506556 1.34536281644779 9 0.23395083922801096 1.1374874650435376 >10 1.188254357433613 11.709772020925431 >50 0.09413520535456782 3.555138813244959 >100 0.05433969451367848 5.238024278379815 >500 0.0038013079353133106 1.3375977293484242 >1k 7.437341612568573E-4 0.5666107196077865 >5k 1.652742580570794E-4 0.5246908121297128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6643 0.2950131541316053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5110 0.2269331954858502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3588 0.15934174274035823 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.440962729664388E-5 2 0.0 0.0 0.0 0.0 4.440962729664388E-5 3 0.0 0.0 0.0 0.0 4.440962729664388E-5 4 0.0 0.0 0.0 0.0 4.440962729664388E-5 5 0.0 0.0 0.0 0.0 4.440962729664388E-5 6 0.0 0.0 0.0 0.0 4.440962729664388E-5 7 0.0 0.0 0.0 0.0 4.440962729664388E-5 8 0.0 0.0 0.0 0.0 4.440962729664388E-5 9 0.0 0.0 0.0 4.440962729664388E-5 4.440962729664388E-5 10-11 0.0 0.0 0.0 4.440962729664388E-5 4.440962729664388E-5 12-13 0.0 0.0 0.0 4.440962729664388E-5 4.440962729664388E-5 14-15 0.0 0.0 0.0 4.440962729664388E-5 4.440962729664388E-5 16-17 0.0 0.0 0.0 8.881925459328775E-5 4.440962729664388E-5 18-19 0.0 0.0 0.0 8.881925459328775E-5 4.440962729664388E-5 20-21 0.0 0.0 0.0 2.442529501315413E-4 4.440962729664388E-5 22-23 0.0 0.0 0.0 4.4409627296643873E-4 4.440962729664388E-5 24-25 0.0 0.0 0.0 9.103973595811994E-4 4.440962729664388E-5 26-27 0.0 0.0 0.0 0.0015321321417342136 4.440962729664388E-5 28-29 0.0 0.0 0.0 0.004907263816279149 4.440962729664388E-5 30-31 0.0 0.0 0.0 0.015210297349100527 4.440962729664388E-5 32-33 0.0 0.0 0.0 0.03512801519164531 4.440962729664388E-5 34-35 0.0 0.0 0.0 0.06521553768512153 4.440962729664388E-5 36-37 0.0 0.0 0.0 0.1171081871812499 4.440962729664388E-5 38-39 0.0 0.0 0.0 0.18838563899236332 4.440962729664388E-5 40-41 0.0 0.0 0.0 0.2705878591184511 4.440962729664388E-5 42-43 0.0 0.0 0.0 0.3559875724098973 4.440962729664388E-5 44-45 0.0 0.0 0.0 0.46163807574861304 4.440962729664388E-5 46-47 0.0 0.0 0.0 0.584541719292075 4.440962729664388E-5 48-49 0.0 0.0 0.0 0.7177483963683584 4.440962729664388E-5 50-51 0.0 0.0 0.0 0.8584825052714227 4.440962729664388E-5 52-53 0.0 0.0 0.0 1.0017479629303958 4.440962729664388E-5 54-55 0.0 0.0 0.0 1.1594687542744266 4.440962729664388E-5 56-57 0.0 0.0 0.0 1.3435910690463122 4.440962729664388E-5 58-59 0.0 0.0 0.0 1.5437008496449893 4.440962729664388E-5 60-61 0.0 0.0 0.0 1.761552276348676 4.440962729664388E-5 62-63 0.0 0.0 0.0 1.9749183306954015 4.440962729664388E-5 64-65 0.0 0.0 0.0 2.202162393572328 4.440962729664388E-5 66-67 0.0 0.0 0.0 2.4597160270792147 4.440962729664388E-5 68-69 0.0 0.0 0.0 2.745092292087448 4.440962729664388E-5 70-71 0.0 0.0 0.0 3.0530286477623765 4.440962729664388E-5 72-73 0.0 0.0 0.0 3.363851629211587 4.440962729664388E-5 74-75 0.0 0.0 0.0 3.6801147900046365 4.440962729664388E-5 76-77 0.0 0.0 0.0 4.02291270310743 4.440962729664388E-5 78-79 0.0 0.0 0.0 4.389936067900544 4.440962729664388E-5 80-81 0.0 0.0 0.0 4.7850485219587835 4.440962729664388E-5 82-83 0.0 0.0 0.0 5.186467143093148 4.440962729664388E-5 84-85 0.0 2.220481364832194E-5 0.0 5.585776306930922 4.440962729664388E-5 86-87 0.0 4.440962729664388E-5 0.0 6.000451201813334 4.440962729664388E-5 88-89 0.0 4.440962729664388E-5 0.0 6.441105728664283 4.440962729664388E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3445 0.0 57.04696 1 GTATCAA 7050 0.0 46.956406 1 ATCAACG 9290 0.0 35.096695 3 TATCAAC 9625 0.0 34.07267 2 TCAACGC 9520 0.0 34.049072 4 CAACGCA 9715 0.0 33.41456 5 AACGCAG 10065 0.0 32.293365 6 ACGCAGA 11545 0.0 27.900383 7 CGCAGAG 11785 0.0 27.291885 8 GTACATG 10000 0.0 26.378471 1 TACATGG 10030 0.0 25.683617 2 GTGGTAT 1790 0.0 25.049515 1 TGGTATC 1635 0.0 24.709217 2 GCAGAGT 12785 0.0 24.487242 9 ACATGGG 10425 0.0 23.981012 3 GTAAGGT 835 0.0 21.629921 4 AGAGTAC 12020 0.0 21.339851 10-11 GAGTACT 6655 0.0 20.87664 12-13 TAAGGTG 1095 0.0 20.834589 5 CATGGGG 5135 0.0 20.640596 4 >>END_MODULE