FastQCFastQC Report
Thu 26 May 2016
SRR938368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251764
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65480.2907942395384241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.24589610634151715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39220.17417455825743727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.057.667691
GTATCAA68350.041.4345661
ATCAACG88550.031.6486023
TATCAAC90500.031.0757852
TCAACGC91300.030.7980164
CAACGCA94400.029.8872685
AACGCAG97850.028.7849646
TGGTATC16300.027.6875782
GTGGTAT19300.026.3439871
ACGCAGA112850.024.7905147
GTACATG96400.024.6953831
CGCAGAG116250.024.0654568
TACATGG97100.024.0221142
ACATGGG99250.022.7807243
GCAGAGT125550.021.6775259
GAGTACT66400.020.95857212-13
TATGACG2056.838527E-720.8526124
GTACTTT69150.020.02204914-15
AGAGTAC117600.019.64887410-11
CAGAGTA121650.019.48276310-11
Produced by FastQC (version 0.11.5)