##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938368_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2251764 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7365638672614 31.0 31.0 34.0 30.0 34.0 2 31.997654727582464 31.0 31.0 34.0 30.0 34.0 3 32.05353225293592 33.0 31.0 34.0 30.0 34.0 4 35.72200861191492 37.0 35.0 37.0 33.0 37.0 5 35.60792338806376 37.0 35.0 37.0 33.0 37.0 6 35.64761804523032 37.0 35.0 37.0 33.0 37.0 7 35.56805597744702 37.0 35.0 37.0 33.0 37.0 8 35.538448967120885 37.0 35.0 37.0 33.0 37.0 9 37.06951572189625 39.0 37.0 39.0 33.0 39.0 10-11 36.99266352957059 39.0 37.0 39.0 33.0 39.0 12-13 36.8623039537003 39.0 37.0 39.0 32.5 39.0 14-15 37.843090794594815 40.0 37.0 41.0 32.5 41.0 16-17 37.933984644927264 40.0 37.0 41.0 33.0 41.0 18-19 38.073428432109225 40.0 37.0 41.0 33.0 41.0 20-21 38.02755839421893 40.0 37.0 41.0 33.0 41.0 22-23 37.89280204319813 40.0 37.0 41.0 32.5 41.0 24-25 37.75955628564983 40.0 37.0 41.0 32.0 41.0 26-27 37.532708356648385 39.0 37.0 41.0 32.0 41.0 28-29 37.378222806652914 39.0 36.0 41.0 31.5 41.0 30-31 37.11214363494576 39.0 36.0 40.5 31.0 41.0 32-33 37.009866264848355 39.0 36.0 40.0 31.0 41.0 34-35 36.86353476652083 39.0 35.5 40.0 30.0 41.0 36-37 36.728759985504695 39.0 35.0 40.0 30.0 41.0 38-39 36.507256755148404 39.0 35.0 40.0 30.0 41.0 40-41 36.270557882620025 38.5 35.0 40.0 30.0 41.0 42-43 35.99337097493343 38.0 35.0 40.0 29.5 41.0 44-45 35.68292347688301 38.0 35.0 40.0 28.5 41.0 46-47 35.23598454367331 38.0 34.0 40.0 27.0 41.0 48-49 34.8115459701816 38.0 33.5 40.0 26.0 41.0 50-51 34.39964845339032 38.0 33.0 40.0 24.0 41.0 52-53 33.769411670139505 37.0 32.5 40.0 23.0 41.0 54-55 33.3034956149934 37.0 32.0 39.5 22.0 40.5 56-57 32.875027089872646 36.0 31.0 39.0 20.5 40.0 58-59 32.295602691933965 36.0 31.0 39.0 18.5 40.0 60-61 31.76355803716553 35.0 30.0 39.0 15.0 40.0 62-63 31.202615149722618 35.0 29.0 38.0 10.5 40.0 64-65 30.49567117157926 34.0 28.0 38.0 8.0 40.0 66-67 29.785975794976736 34.0 28.0 37.5 6.0 39.5 68-69 29.012765769414557 33.5 26.0 36.5 2.0 39.0 70-71 28.191494979047537 33.0 26.0 36.0 2.0 39.0 72-73 27.55626055838889 32.0 25.0 35.5 2.0 38.0 74-75 26.79243450912263 31.5 23.5 35.0 2.0 37.5 76-77 25.66810842521685 30.5 22.5 34.0 2.0 36.0 78-79 26.138970824651253 31.0 22.5 34.5 2.0 36.0 80-81 25.70776155938189 31.0 21.5 34.5 2.0 36.0 82-83 24.96912531686269 31.0 19.0 34.0 2.0 36.0 84-85 24.126791040268873 30.5 14.5 34.0 2.0 35.0 86-87 23.13656715357382 29.5 6.5 34.0 2.0 35.0 88-89 22.08381717622273 29.0 2.0 34.0 2.0 35.0 90-91 21.145970892153883 28.5 2.0 33.5 2.0 35.0 92-93 19.979343083911104 26.5 2.0 33.0 2.0 34.0 94-95 18.721255646684114 25.0 2.0 32.0 2.0 34.0 96-97 17.264518173307685 21.0 2.0 32.0 2.0 34.0 98-99 15.467449075480378 2.0 2.0 31.5 2.0 34.0 100-101 11.522281420255409 2.0 2.0 25.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31628163557083866 1101 2 0.34567768270202137 1101 3 0.35672974846107763 1101 4 0.1920669565655757 1101 5 0.1347236302544772 1101 6 0.2588117362973321 1101 7 0.3024485238820631 1101 8 0.24351085109203296 1101 9 0.2914446797396337 1101 10-11 0.3235852639678285 1101 12-13 0.36733558294047697 1101 14-15 0.49159912363735714 1101 16-17 0.38282797424169246 1101 18-19 0.3965831391482837 1101 20-21 0.5178210561318224 1101 22-23 0.5511602827353173 1101 24-25 0.41043467018715774 1101 26-27 0.5877302417317622 1101 28-29 0.6111354158249682 1101 30-31 0.6755644474778819 1101 32-33 0.6390036782984652 1101 34-35 0.30138546244864983 1101 36-37 0.4397002500639786 1101 38-39 0.7451713234507835 1101 40-41 0.7210330016626401 1101 42-43 0.8818711452895869 1101 44-45 0.8368146663209259 1101 46-47 0.9027943104327534 1101 48-49 0.9569284632228232 1101 50-51 1.1461525292562627 1101 52-53 1.099362171882703 1101 54-55 1.204081660939032 1101 56-57 1.3833608028571334 1101 58-59 1.4325118391418172 1101 60-61 1.4177880135239214 1101 62-63 1.5094954959802358 1101 64-65 1.609626649460214 1101 66-67 1.7700593976450953 1101 68-69 1.8337848431764954 1101 70-71 2.204605550246729 1101 72-73 2.368966367942594 1101 74-75 2.1588940585215113 1101 76-77 2.2055317029657253 1101 78-79 2.3188186597522673 1101 80-81 2.583330570459129 1101 82-83 2.650575851097283 1101 84-85 2.880016361116695 1101 86-87 2.8369350230052 1101 88-89 2.755811944262529 1101 90-91 3.0621972085813347 1101 92-93 3.352622977791153 1101 94-95 3.164710644113004 1101 96-97 3.2686221779861704 1101 98-99 3.536321910621112 1101 100-101 2.8908058235237863 1102 1 0.3299642759196786 1102 2 0.26880307828044536 1102 3 0.2502424212831329 1102 4 0.22859733221797995 1102 5 0.19427786306291495 1102 6 0.20235295437673528 1102 7 0.3186958641637929 1102 8 0.25786100518871535 1102 9 0.363662988142913 1102 10-11 0.3310006698193675 1102 12-13 0.31753325288413947 1102 14-15 0.5293947539070984 1102 16-17 0.3694707418753467 1102 18-19 0.3950676165712963 1102 20-21 0.37116005610955227 1102 22-23 0.42533314613473294 1102 24-25 0.43463929913062316 1102 26-27 0.47051478785422063 1102 28-29 0.5318642819978052 1102 30-31 0.4779004672989444 1102 32-33 0.5487684621973443 1102 34-35 0.5145408106689828 1102 36-37 0.4603178374410888 1102 38-39 0.6298397372886342 1102 40-41 0.7303014638445475 1102 42-43 0.6464978067189833 1102 44-45 0.5836234019648074 1102 46-47 0.6312766330466388 1102 48-49 0.6278523356454002 1102 50-51 0.7062307543932604 1102 52-53 0.7056536976783931 1102 54-55 0.7080990679800934 1102 56-57 0.8853784516184717 1102 58-59 0.9531779296024538 1102 60-61 1.0352585061562287 1102 62-63 1.2110494632144118 1102 64-65 1.2611932195447828 1102 66-67 1.3149750420363908 1102 68-69 1.4483081771353348 1102 70-71 1.7863499437673518 1102 72-73 1.7062854355384012 1102 74-75 1.7791558638539904 1102 76-77 1.7713633082110576 1102 78-79 1.8444368355274463 1102 80-81 1.9990596433664187 1102 82-83 2.0791948172341783 1102 84-85 2.491959887624823 1102 86-87 2.326063176103194 1102 88-89 2.205363370253181 1102 90-91 2.386286265013478 1102 92-93 2.4229240229133318 1102 94-95 2.4539331396841177 1102 96-97 2.4547535561416503 1102 98-99 2.4759333850194345 1102 100-101 2.03469121471308 1103 1 0.3365364061459388 1103 2 0.2936471855346561 1103 3 0.32592392278046844 1103 4 0.2223237077308866 1103 5 0.24924429800752534 1103 6 0.23626732499345593 1103 7 0.2959357157385014 1103 8 0.3317428115429806 1103 9 0.42512726368430265 1103 10-11 0.4420628960447601 1103 12-13 0.5182803833028586 1103 14-15 0.5899813147976545 1103 16-17 0.4867435618788889 1103 18-19 0.504525109815944 1103 20-21 0.5310334558031613 1103 22-23 0.5575073880340895 1103 24-25 0.5312858729544985 1103 26-27 0.6717801173075557 1103 28-29 0.5713927160647998 1103 30-31 0.3727288698024651 1103 32-33 0.4715967415263549 1103 34-35 0.5709788923798769 1103 36-37 0.5821321403118205 1103 38-39 0.6530862325887554 1103 40-41 0.6853375693743118 1103 42-43 0.7771339068598593 1103 44-45 0.9097327424422659 1103 46-47 0.854778234315333 1103 48-49 0.8997174480036563 1103 50-51 0.8103318883686441 1103 52-53 0.9035224898752858 1103 54-55 1.0139007801058497 1103 56-57 1.2483308292715307 1103 58-59 1.513163629811082 1103 60-61 1.4490421193266663 1103 62-63 1.5968098156475001 1103 64-65 1.5745110790796453 1103 66-67 1.5039716123959828 1103 68-69 1.6569355896236893 1103 70-71 1.8487393753939472 1103 72-73 1.945985736546124 1103 74-75 1.7304824918746498 1103 76-77 1.948659839308771 1103 78-79 1.8918860645319668 1103 80-81 2.131575983235699 1103 82-83 2.3626488231003613 1103 84-85 2.5336021118486336 1103 86-87 2.4541431163635536 1103 88-89 2.428192515653002 1103 90-91 2.8831521472124493 1103 92-93 3.2005096292013953 1103 94-95 2.9305772307716715 1103 96-97 2.897616940207488 1103 98-99 3.103672959451483 1103 100-101 2.7600321579219305 1104 1 0.24380816180837073 1104 2 0.2423214980269499 1104 3 0.263515898774223 1104 4 0.19683762231139212 1104 5 0.16953862118752738 1104 6 0.10334505755398027 1104 7 0.14540338540594888 1104 8 0.15790304502840513 1104 9 0.08336707952678069 1104 10-11 0.20352957915406478 1104 12-13 0.2346695804911647 1104 14-15 0.3588677372911775 1104 16-17 0.297969771440286 1104 18-19 0.34588656005642093 1104 20-21 0.4174107320805476 1104 22-23 0.4166515488430633 1104 24-25 0.3775184540372436 1104 26-27 0.532811070569359 1104 28-29 0.37570479729141226 1104 30-31 0.40358347579580567 1104 32-33 0.5821734669754477 1104 34-35 0.34773311608194035 1104 36-37 0.4968935165604407 1104 38-39 0.5183963818056725 1104 40-41 0.6502818699456938 1104 42-43 0.5737919411427583 1104 44-45 0.5753922976689623 1104 46-47 0.5898791509922745 1104 48-49 0.6989158695106283 1104 50-51 0.7625824741375382 1104 52-53 0.8235907268136629 1104 54-55 1.1027444517464104 1104 56-57 1.2495640269531236 1104 58-59 1.2975307938674803 1104 60-61 1.208317821221673 1104 62-63 1.2841572074344008 1104 64-65 1.437034201536175 1104 66-67 1.536195067815889 1104 68-69 1.5436458292751034 1104 70-71 1.3886456523701476 1104 72-73 1.8111150167697438 1104 74-75 1.755339646476532 1104 76-77 1.9242795211437702 1104 78-79 2.000119714386038 1104 80-81 2.3329694966158954 1104 82-83 2.3689257992996495 1104 84-85 2.5476687867366152 1104 86-87 2.573816730043827 1104 88-89 2.5360192102721477 1104 90-91 2.7537938210727724 1104 92-93 2.7890244481268667 1104 94-95 2.716321465572509 1104 96-97 2.9886474824034046 1104 98-99 2.9523267186490827 1104 100-101 2.3039216634096604 1105 1 0.13448626709390155 1105 2 0.227465170206532 1105 3 0.16899254156066235 1105 4 0.16590627598138497 1105 5 0.03357134912774029 1105 6 0.08680121727307721 1105 7 0.10291889349966965 1105 8 0.13596046471698742 1105 9 0.18798617720735677 1105 10-11 0.1853623402929685 1105 12-13 0.23752870824377936 1105 14-15 0.3976620801634283 1105 16-17 0.3072027849946579 1105 18-19 0.33569640531361955 1105 20-21 0.2433962634221345 1105 22-23 0.3458070285158854 1105 24-25 0.20355395907457563 1105 26-27 0.16997129993897175 1105 28-29 0.3070076528182497 1105 30-31 0.48506475234707835 1105 32-33 0.5515331800965697 1105 34-35 0.5811330224581042 1105 36-37 0.5486775072366967 1105 38-39 0.5443786635396322 1105 40-41 0.5747528206491452 1105 42-43 0.6709462509568311 1105 44-45 0.63783877794868 1105 46-47 0.754479594168501 1105 48-49 0.7265717870022144 1105 50-51 0.7482214919128936 1105 52-53 0.6963883491548089 1105 54-55 0.8389116185891723 1105 56-57 1.0588721327919686 1105 58-59 1.0844611354539424 1105 60-61 1.0295070195259122 1105 62-63 1.186810116843251 1105 64-65 1.4524230258720472 1105 66-67 1.568233915557716 1105 68-69 1.688188025430204 1105 70-71 1.5090765503421295 1105 72-73 1.5660000113991082 1105 74-75 1.582542990234959 1105 76-77 1.6670300417396966 1105 78-79 1.8220197863165382 1105 80-81 1.8392879634334491 1105 82-83 2.044873345362838 1105 84-85 2.2643962737623404 1105 86-87 2.284440487837397 1105 88-89 2.135297358241715 1105 90-91 2.3367070328356476 1105 92-93 2.556372039333251 1105 94-95 2.542605346025592 1105 96-97 2.660075263386876 1105 98-99 2.3318189718479694 1105 100-101 1.9641527895567936 1106 1 0.08960050429982047 1106 2 0.04876792856871148 1106 3 0.11662590649793714 1106 4 0.09374946165819154 1106 5 0.12902047046480902 1106 6 0.08627168229192961 1106 7 0.1117412426276232 1106 8 0.09595975914073307 1106 9 -0.06089773344905325 1106 10-11 0.1866142089675904 1106 12-13 0.17377539534276565 1106 14-15 0.11528437637315392 1106 16-17 0.18567671174702127 1106 18-19 0.26214740083761257 1106 20-21 0.22156344089660962 1106 22-23 0.16571653836088274 1106 24-25 0.198893716091753 1106 26-27 0.07617640213727839 1106 28-29 0.22534707909655793 1106 30-31 0.3374870397371055 1106 32-33 0.05530686651202643 1106 34-35 -0.028110202907356552 1106 36-37 0.05624428341467791 1106 38-39 0.2122496312815656 1106 40-41 0.08751942925276524 1106 42-43 0.2280922831928507 1106 44-45 0.1491612483475464 1106 46-47 -0.31412349713748 1106 48-49 0.05572707992659787 1106 50-51 0.3256975574432843 1106 52-53 0.21523720149092895 1106 54-55 0.20773176279131889 1106 56-57 0.30552518771666826 1106 58-59 0.7027882141477377 1106 60-61 0.6458365236380921 1106 62-63 0.6794572184682757 1106 64-65 1.1049644674525396 1106 66-67 1.0678230055848381 1106 68-69 0.8057043088072682 1106 70-71 0.785764487193056 1106 72-73 1.16519821217965 1106 74-75 1.1100041063684039 1106 76-77 1.1744995365911954 1106 78-79 1.3138555634426368 1106 80-81 1.3111122749453656 1106 82-83 1.5502167625568468 1106 84-85 1.8109771835147264 1106 86-87 1.8117979724580415 1106 88-89 1.7726993382650846 1106 90-91 2.0447120600664803 1106 92-93 1.83353244768551 1106 94-95 1.7680275231693336 1106 96-97 1.9732830335454103 1106 98-99 1.9835940694159362 1106 100-101 1.8932444042239602 1107 1 0.027084474812419046 1107 2 0.1285580880063968 1107 3 0.08303238686823988 1107 4 0.08972203151932234 1107 5 -0.01619537044038566 1107 6 -0.007363496861167107 1107 7 0.013045031415551023 1107 8 0.11153442054995111 1107 9 0.12459892350277357 1107 10-11 0.08139416782238129 1107 12-13 0.07476656626681688 1107 14-15 0.1983931540175945 1107 16-17 0.2501854551037823 1107 18-19 0.21304925237199512 1107 20-21 0.24274662279272263 1107 22-23 0.27536148950094486 1107 24-25 0.20488394410477895 1107 26-27 0.2048396114635267 1107 28-29 0.22361075130640273 1107 30-31 0.36972808208238206 1107 32-33 0.4002060607516853 1107 34-35 0.43617284183805793 1107 36-37 0.3990098414465706 1107 38-39 0.31835549447374945 1107 40-41 0.2667442706722838 1107 42-43 0.12416265864287368 1107 44-45 0.2557143942416005 1107 46-47 0.4511351176405043 1107 48-49 0.36260518724701996 1107 50-51 0.4712843909766349 1107 52-53 0.5283184632773157 1107 54-55 0.5218004238576697 1107 56-57 0.5673795508231336 1107 58-59 0.6204325652535161 1107 60-61 0.769327951719692 1107 62-63 1.041976248127117 1107 64-65 1.2469999551946955 1107 66-67 1.19658753498652 1107 68-69 1.3811005885946877 1107 70-71 1.2019126099429265 1107 72-73 1.703041774668936 1107 74-75 1.715730747890774 1107 76-77 1.6863173833252922 1107 78-79 1.7656242930843327 1107 80-81 1.796698079848504 1107 82-83 2.0821917325551347 1107 84-85 2.3017046822289373 1107 86-87 2.641993155039902 1107 88-89 2.6397168249786134 1107 90-91 2.723944959089284 1107 92-93 2.7881628265643066 1107 94-95 2.593991092516152 1107 96-97 2.620277376079269 1107 98-99 2.6725442665700605 1107 100-101 2.1167251174075705 1108 1 0.08022359428284176 1108 2 0.08185876048721852 1108 3 0.051501967618477806 1108 4 0.06979190647992084 1108 5 0.07848651460742673 1108 6 0.13851361777579996 1108 7 0.1687913275172619 1108 8 0.18354634657706015 1108 9 0.13335693548133776 1108 10-11 0.20197838553805525 1108 12-13 0.10691758549556596 1108 14-15 0.23673047144956172 1108 16-17 0.19821660987944512 1108 18-19 0.1151868745218323 1108 20-21 0.05936819471664734 1108 22-23 0.20847460287701125 1108 24-25 0.26803081286594477 1108 26-27 0.32487546835120185 1108 28-29 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-0.11503333181369513 2316 70-71 -0.23127217407188994 2316 72-73 0.0464765888442038 2316 74-75 0.15093292104230116 2316 76-77 0.017284665410567612 2316 78-79 -0.3912385909527174 2316 80-81 -0.6283946181239877 2316 82-83 -0.042519562861784266 2316 84-85 0.14800219584474306 2316 86-87 0.30891645809650115 2316 88-89 0.3379649386784358 2316 90-91 0.3413457057603644 2316 92-93 0.09093144719231105 2316 94-95 0.3354857537524829 2316 96-97 0.35443768724793756 2316 98-99 0.16671466877242835 2316 100-101 0.08640168833114892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 14.0 4 39.0 5 96.0 6 216.0 7 508.0 8 1190.0 9 2138.0 10 3265.0 11 5211.0 12 7484.0 13 10519.0 14 14442.0 15 19293.0 16 24461.0 17 30236.0 18 30519.0 19 29043.0 20 30718.0 21 34153.0 22 37358.0 23 41570.0 24 46756.0 25 53401.0 26 60831.0 27 71035.0 28 82238.0 29 96173.0 30 111448.0 31 126915.0 32 147212.0 33 172566.0 34 203278.0 35 237076.0 36 258074.0 37 205861.0 38 55687.0 39 729.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.012020936026786 19.66321656652181 15.222817301939065 23.10194519551234 2 16.01003775260937 20.84832333999556 39.07936930934932 24.062269598045745 3 19.358874321798336 24.42520785157348 29.983075212936562 26.232842613691627 4 13.496041325822777 17.262244178341955 36.13558081575156 33.1061336800837 5 14.078111413393446 37.47058964595663 33.81047164038054 14.640827300269391 6 32.545817412481945 36.09419104311109 16.997207522635588 14.362784021771377 7 28.962848682188717 32.53400445162104 20.190082086755094 18.313064779435145 8 26.616821300988914 35.36875978122041 19.372323209714697 18.642095708075978 9 26.538852514856163 16.628600129942857 19.77631709254854 37.05623026265244 10-11 25.24864950323391 26.712057746726565 26.988618700716415 21.05067404932311 12-13 25.985538448967123 23.827830092318735 27.594943342197496 22.59168811651665 14-15 22.67859775713618 25.261683728845476 25.82413165855747 26.235586855460873 16-17 22.533289456621564 28.66092983101249 26.34130397324054 22.464476739125413 18-19 22.396485599734252 26.969566970606156 28.836947388802734 21.797000040856858 20-21 23.13279722031261 26.186070121025118 28.77006649009399 21.911066168568286 22-23 22.82144132333584 26.1763222078335 28.6302871881778 22.371949280652856 24-25 22.62073627075408 26.338617576028344 28.774615561573658 22.266030591643922 26-27 22.32037850955423 26.813958094980922 28.481929037897146 22.383734357567704 28-29 22.309790663979882 26.859582216152905 28.719969357336755 22.11065776253046 30-31 22.445935719729064 26.50788448523025 28.603552592545224 22.442627202495466 32-33 22.153969271516942 26.58182763707145 28.508836109797937 22.75536698161367 34-35 22.841493843752747 26.66972828302837 28.26377483084866 22.225003042370222 36-37 22.791312291466173 26.312022784228855 28.32320705874098 22.573457865563988 38-39 22.43717964120926 26.65711590978288 28.44060983011393 22.465094618893932 40-41 22.739960332785326 26.382695127630406 28.492505934425843 22.384838605158425 42-43 22.629313359854596 26.421417159175313 28.19809006940458 22.751179411565506 44-45 22.50010047760726 26.64464079088867 28.243222868659 22.61203586284507 46-47 22.691180797264366 26.76170333238963 27.703943022410222 22.843172847935783 48-49 22.719476819062745 26.845797339330407 27.684517560454825 22.75020828115202 50-51 22.733443646847537 26.9346165939237 27.560814543620022 22.77112521560874 52-53 23.10377552887425 26.829721054249024 27.393789935357347 22.672713481519377 54-55 22.61868916991301 26.79388248502063 27.643327631137186 22.94410071392917 56-57 22.694079551427137 27.086459124148693 27.461587329219967 22.757873995204204 58-59 22.697338619855365 26.678173201099227 27.53754389891658 23.086944280128822 60-61 22.625106361057377 26.814755009850057 27.687559619924645 22.87257900916792 62-63 22.640205634338233 26.87541856073727 27.624719997299895 22.859655807624602 64-65 22.334137074463705 26.70877888266586 27.594862955068734 23.362221087801704 66-67 22.34140113245254 26.992183857000114 27.458243588320197 23.208171422227156 68-69 22.530146646397924 26.717302995945808 27.528763273606266 23.223787084050002 70-71 22.357770333734056 26.797802433026924 27.35227908088018 23.49214815235884 72-73 22.59227119776139 26.770493645190268 27.21670036624701 23.420534790801327 74-75 22.514481979461433 26.894781158238608 27.03924567583459 23.551491186465366 76-77 22.381008844621373 26.74012463117805 27.485140538706542 23.39372598549404 78-79 22.443071298768434 26.878171957629664 27.30517052408689 23.373586219515012 80-81 22.330389415217688 27.05931046856216 27.18905336756432 23.42124674865583 82-83 22.534480063382208 26.97805160107579 27.092785299875295 23.394683035666702 84-85 22.49350362113998 26.707456213007504 27.104500321765613 23.694539844086908 86-87 22.483327363686083 26.752872369978085 27.108541954555033 23.6552583117808 88-89 22.29703023940342 26.813844612490474 26.937814087089055 23.95131106101705 90-91 22.30867155193336 26.750798772414093 27.142651904430203 23.797877771222343 92-93 22.39220340601376 26.54192678813502 27.07213603626509 23.993733769586136 94-95 22.166436291571603 26.44701650373874 27.047101992328233 24.339445212361426 96-97 22.33447251454249 26.317839329041437 27.021776487628475 24.325911668787597 98-99 22.102982372930732 26.359156643413783 26.83227460781858 24.705586375836898 100-101 22.299250720768253 26.08665917032158 27.092603843031508 24.52148626587866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 534.0 1 366.5 2 239.0 3 333.0 4 325.0 5 305.5 6 543.0 7 1198.5 8 1803.5 9 1993.0 10 1747.0 11 1480.0 12 1536.5 13 1685.5 14 1904.0 15 2125.5 16 2269.5 17 2394.0 18 2610.0 19 2874.5 20 3218.0 21 3755.5 22 4475.0 23 5283.0 24 6430.5 25 8005.5 26 10005.0 27 12784.5 28 15612.0 29 18800.5 30 22551.5 31 26428.0 32 31453.0 33 35472.5 34 38849.5 35 43154.0 36 47783.0 37 52645.5 38 57097.5 39 60304.0 40 63901.5 41 69177.5 42 73828.0 43 78450.0 44 82008.5 45 84352.5 46 85702.0 47 87524.0 48 91425.5 49 92807.0 50 91106.5 51 88107.5 52 83885.0 53 81342.5 54 79920.5 55 74051.0 56 66216.0 57 59759.5 58 53652.0 59 46705.0 60 38972.0 61 32220.0 62 26436.0 63 20701.5 64 15740.0 65 12310.0 66 9355.0 67 7154.0 68 5358.5 69 3766.0 70 2910.5 71 2347.5 72 1925.5 73 1650.5 74 1407.5 75 1178.5 76 861.0 77 554.5 78 350.5 79 204.5 80 129.0 81 87.5 82 49.5 83 31.5 84 15.5 85 7.0 86 4.5 87 1.5 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023315054330738036 2 0.011724141606313984 3 0.0016431562099758234 4 0.0 5 2.6645776377986325E-4 6 0.0 7 0.0 8 0.0 9 2.2204813648321937E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001687565837272467 26-27 0.0087042869501422 28-29 6.661444094496581E-5 30-31 0.0 32-33 4.218914593181168E-4 34-35 0.01021421427822809 36-37 0.0013766984461959602 38-39 0.012634538965895182 40-41 0.012545719711301896 42-43 0.004951673443575793 44-45 8.215781049879117E-4 46-47 6.661444094496581E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.220481364832194E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1102406824160968E-4 66-67 6.217347821530143E-4 68-69 9.326021732295214E-4 70-71 4.440962729664388E-5 72-73 2.2204813648321937E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3322888188993162E-4 82-83 0.0012434695643060286 84-85 0.0055512034120804845 86-87 0.00424111940682949 88-89 0.0 90-91 0.0012212647506577065 92-93 5.107107139114045E-4 94-95 6.661444094496581E-5 96-97 2.220481364832194E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2251764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74583662954376 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66273603227768 38.11739904411448 2 14.3634889807018 14.865015084512947 3 5.157440084558126 8.006281561266155 4 2.3004325791064417 4.761512336628877 5 1.2265477362584303 3.1734369389382735 6 0.7354975859787082 2.2835362755286823 7 0.49596120087197754 1.7964749092439578 8 0.3275193774852538 1.3558211360289452 9 0.23962028506911534 1.1159416911880031 >10 1.3180221824880851 12.406788043877052 >50 0.10606317748625463 3.803586858486891 >100 0.06142006019498916 5.7185983685034945 >500 0.0042177894857566445 1.4297801563133392 >1k 8.607733644400866E-4 0.6275443184516452 >5k 1.7215467288801732E-4 0.5382832769173207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6548 0.2907942395384241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.24589610634151715 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3922 0.17417455825743727 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.440962729664388E-5 0.0 10-11 0.0 0.0 0.0 4.440962729664388E-5 0.0 12-13 0.0 0.0 0.0 4.440962729664388E-5 0.0 14-15 0.0 0.0 0.0 4.440962729664388E-5 0.0 16-17 0.0 0.0 0.0 8.881925459328775E-5 0.0 18-19 0.0 0.0 0.0 8.881925459328775E-5 0.0 20-21 0.0 0.0 0.0 2.442529501315413E-4 0.0 22-23 0.0 0.0 0.0 4.4409627296643873E-4 0.0 24-25 0.0 0.0 0.0 9.103973595811994E-4 0.0 26-27 0.0 0.0 0.0 0.0014877225144375697 0.0 28-29 0.0 0.0 0.0 0.004840649375334183 0.0 30-31 0.0 0.0 0.0 0.015165887721803883 0.0 32-33 0.0 0.0 0.0 0.03521683444623859 0.0 34-35 0.0 0.0 0.0 0.06530435693971481 0.0 36-37 0.0 0.0 0.0 0.11693054867206333 0.0 38-39 0.0 0.0 0.0 0.18803036197399015 0.0 40-41 0.0 0.0 0.0 0.2708765216958793 0.0 42-43 0.0 0.0 0.0 0.3567869457012369 0.0 44-45 0.0 0.0 0.0 0.4625040634808977 0.0 46-47 0.0 0.0 0.0 0.5857185744154361 0.0 48-49 0.0 0.0 0.0 0.7186587937279395 0.0 50-51 0.0 0.0 0.0 0.8590598304262791 0.0 52-53 0.0 0.0 0.0 1.0015037099802644 0.0 54-55 0.0 0.0 0.0 1.158314103964714 0.0 56-57 0.0 0.0 0.0 1.3420145272772812 0.0 58-59 0.0 0.0 0.0 1.5415469827211021 0.0 60-61 0.0 0.0 0.0 1.7583991928106144 0.0 62-63 0.0 0.0 0.0 1.9707216209158687 0.0 64-65 4.440962729664388E-5 2.220481364832194E-5 0.0 2.1967444190421377 0.0 66-67 4.440962729664388E-5 4.440962729664388E-5 0.0 2.453765137021464 0.0 68-69 4.440962729664388E-5 4.440962729664388E-5 0.0 2.7400295945756303 0.0 70-71 4.440962729664388E-5 4.440962729664388E-5 0.0 3.050830371211193 4.440962729664388E-5 72-73 4.440962729664388E-5 4.440962729664388E-5 0.0 3.3644955688073885 4.440962729664388E-5 74-75 4.440962729664388E-5 4.440962729664388E-5 0.0 3.6869538726083197 4.440962729664388E-5 76-77 4.440962729664388E-5 4.440962729664388E-5 0.0 4.039277650766244 4.440962729664388E-5 78-79 4.440962729664388E-5 4.440962729664388E-5 0.0 4.416204362446509 4.440962729664388E-5 80-81 4.440962729664388E-5 4.440962729664388E-5 0.0 4.828792004845979 4.440962729664388E-5 82-83 4.440962729664388E-5 4.440962729664388E-5 0.0 5.246175886993486 4.440962729664388E-5 84-85 4.440962729664388E-5 4.440962729664388E-5 0.0 5.664381347245982 4.440962729664388E-5 86-87 4.440962729664388E-5 4.440962729664388E-5 0.0 6.098441044443378 4.440962729664388E-5 88-89 4.440962729664388E-5 4.440962729664388E-5 0.0 6.566496311336357 4.440962729664388E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3675 0.0 57.66769 1 GTATCAA 6835 0.0 41.434566 1 ATCAACG 8855 0.0 31.648602 3 TATCAAC 9050 0.0 31.075785 2 TCAACGC 9130 0.0 30.798016 4 CAACGCA 9440 0.0 29.887268 5 AACGCAG 9785 0.0 28.784964 6 TGGTATC 1630 0.0 27.687578 2 GTGGTAT 1930 0.0 26.343987 1 ACGCAGA 11285 0.0 24.790514 7 GTACATG 9640 0.0 24.695383 1 CGCAGAG 11625 0.0 24.065456 8 TACATGG 9710 0.0 24.022114 2 ACATGGG 9925 0.0 22.780724 3 GCAGAGT 12555 0.0 21.677525 9 GAGTACT 6640 0.0 20.958572 12-13 TATGACG 205 6.838527E-7 20.852612 4 GTACTTT 6915 0.0 20.022049 14-15 AGAGTAC 11760 0.0 19.648874 10-11 CAGAGTA 12165 0.0 19.482763 10-11 >>END_MODULE