FastQCFastQC Report
Thu 26 May 2016
SRR938367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792746
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.188166055917724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.1548654979722467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.044.5398671
GTATCAA84800.038.4751931
ATCAACG104150.030.4581493
TCAACGC105400.030.0969314
TATCAAC106600.029.9812032
CAACGCA106450.029.6660275
AACGCAG109800.028.8247956
GTACATG114750.025.6560921
TACATGG115450.025.2112542
ACGCAGA126450.024.6155197
CGCAGAG128150.024.2889778
GTTCGCG1201.7065214E-423.7605537
ACATGGG121000.023.4259853
GCAGAGT139050.021.4960379
GAGTACT63200.020.97507912-13
CATGGGG68700.020.2145044
GTACTTT65850.020.01916114-15
AGTACTT64950.019.0931612-13
AGAGTAC126550.019.0002510-11
TGGTATC28650.018.426182
Produced by FastQC (version 0.11.5)