##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792746 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.081536237094245 31.0 31.0 34.0 28.0 34.0 2 31.119782823070913 31.0 31.0 34.0 27.0 34.0 3 31.1664454984449 31.0 31.0 34.0 27.0 34.0 4 34.81464372341774 37.0 35.0 37.0 32.0 37.0 5 34.790604659356774 37.0 35.0 37.0 32.0 37.0 6 34.80522253008329 37.0 35.0 37.0 32.0 37.0 7 34.712935583830394 37.0 35.0 37.0 31.0 37.0 8 34.67870654903812 37.0 35.0 37.0 30.0 37.0 9 36.1656577433107 39.0 35.0 39.0 31.0 39.0 10-11 35.99439619643175 39.0 35.0 39.0 30.0 39.0 12-13 35.854856653630506 38.0 35.0 39.0 30.0 39.0 14-15 36.81800027643044 39.0 36.0 41.0 30.5 41.0 16-17 36.64646570078339 39.0 36.0 41.0 30.0 41.0 18-19 36.777629795190826 39.0 36.0 41.0 30.0 41.0 20-21 36.74486007678465 39.0 36.0 41.0 30.0 41.0 22-23 36.65402671778959 39.0 36.0 41.0 30.0 41.0 24-25 36.5152199662984 39.0 36.0 41.0 29.5 41.0 26-27 36.31112066761531 39.0 35.5 41.0 29.0 41.0 28-29 36.10477000056575 39.0 35.0 40.0 28.5 41.0 30-31 35.87484880472481 38.5 35.0 40.0 27.0 41.0 32-33 35.63944966710184 38.0 34.5 40.0 27.0 41.0 34-35 35.38584156955197 38.0 34.0 40.0 26.5 41.0 36-37 35.12456467577073 38.0 34.0 40.0 25.5 41.0 38-39 35.031975876073226 38.0 34.0 40.0 25.0 41.0 40-41 34.94310689192644 38.0 34.0 40.0 25.0 41.0 42-43 34.67375926776012 38.0 33.5 40.0 24.0 41.0 44-45 34.35181717205933 38.0 33.0 40.0 24.0 41.0 46-47 33.98795790952704 38.0 33.0 40.0 23.0 41.0 48-49 33.66226019122398 38.0 33.0 40.0 21.0 41.0 50-51 32.97534183201766 37.0 31.5 39.5 19.5 40.5 52-53 33.00864418031571 37.0 32.0 39.0 19.5 40.0 54-55 33.115539687461734 37.0 32.0 40.0 18.5 41.0 56-57 32.80605343271461 37.0 31.5 40.0 16.5 41.0 58-59 32.4437464058672 36.5 31.0 39.5 13.5 41.0 60-61 31.96906431877443 36.0 31.0 39.0 10.0 40.5 62-63 31.400378337306723 35.0 30.0 39.0 7.5 40.0 64-65 30.880572561915763 35.0 29.0 38.5 6.0 40.0 66-67 30.347730334230178 35.0 28.5 38.0 2.0 40.0 68-69 29.771091427577016 34.0 28.0 37.5 2.0 40.0 70-71 29.143539190459855 34.0 27.5 37.0 2.0 39.0 72-73 28.508391203496487 34.0 26.0 36.0 2.0 39.0 74-75 27.82187155580923 33.0 26.0 36.0 2.0 38.5 76-77 27.070019257032325 32.5 24.5 35.0 2.0 37.5 78-79 26.28770572046294 32.0 22.5 35.0 2.0 37.0 80-81 25.588342262418422 31.5 20.0 35.0 2.0 36.0 82-83 24.857205238141958 31.0 18.0 34.0 2.0 36.0 84-85 24.146934415088232 31.0 12.0 34.0 2.0 35.0 86-87 23.381649638026516 30.0 6.0 34.0 2.0 35.0 88-89 22.560081726014467 29.5 2.0 34.0 2.0 35.0 90-91 21.646531764793504 29.0 2.0 34.0 2.0 35.0 92-93 20.704611518555573 28.0 2.0 34.0 2.0 35.0 94-95 19.692050941976106 26.5 2.0 33.0 2.0 35.0 96-97 18.432116096487114 25.0 2.0 33.0 2.0 34.0 98-99 16.918857461437597 17.5 2.0 32.0 2.0 34.0 100-101 13.437395130097759 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41763532341067844 1101 2 0.25956053390048694 1101 3 0.2698952153296297 1101 4 0.23112917588566972 1101 5 0.2419358311474511 1101 6 0.41708392439768716 1101 7 0.3331488069762969 1101 8 0.46995917006425003 1101 9 0.5838766608349388 1101 10-11 0.5870847663097933 1101 12-13 0.7375984472683399 1101 14-15 0.8513733434395405 1101 16-17 0.8028869240325704 1101 18-19 0.8333876291080102 1101 20-21 0.6849826439128606 1101 22-23 0.8251470402331549 1101 24-25 0.760874100102356 1101 26-27 0.8002676884197584 1101 28-29 0.8872403542515244 1101 30-31 0.7201276770729095 1101 32-33 0.6234870274976672 1101 34-35 0.6522828225089299 1101 36-37 0.7724033768630107 1101 38-39 0.782161374846531 1101 40-41 0.7959892521890524 1101 42-43 0.9625429983608811 1101 44-45 0.8190732324110428 1101 46-47 0.7522333789605824 1101 48-49 0.7149472249184115 1101 50-51 0.8606007133017002 1101 52-53 0.942875412343831 1101 54-55 1.032458326103928 1101 56-57 0.869281756715047 1101 58-59 0.9012317469778992 1101 60-61 0.7766989939340831 1101 62-63 0.7730065197997185 1101 64-65 0.8626289066241704 1101 66-67 0.9905546903516189 1101 68-69 1.0223254000804083 1101 70-71 0.9975299085056086 1101 72-73 1.124106500966299 1101 74-75 1.1118730955269598 1101 76-77 1.096359699487639 1101 78-79 1.3451999077293237 1101 80-81 1.311255166292888 1101 82-83 1.317272408523614 1101 84-85 1.2029137472105056 1101 86-87 1.3255962190518105 1101 88-89 1.4359745962087374 1101 90-91 1.2082425541512798 1101 92-93 1.3020887959343774 1101 94-95 1.05986006112947 1101 96-97 1.1215878295956365 1101 98-99 1.063609860438273 1101 100-101 0.838222429633424 1102 1 0.4972672429044245 1102 2 0.42625750982642785 1102 3 0.40734671861571314 1102 4 0.38142800695096923 1102 5 0.3497605272534514 1102 6 0.41850838362049103 1102 7 0.41297336877961754 1102 8 0.3927357352184444 1102 9 0.6077362008907912 1102 10-11 0.6903482607288609 1102 12-13 0.6143081287750007 1102 14-15 0.8476228954474223 1102 16-17 0.8275284277850687 1102 18-19 0.7532597468227848 1102 20-21 0.8132493021932135 1102 22-23 0.9073936840714012 1102 24-25 0.8180328812720816 1102 26-27 0.6492442457649545 1102 28-29 0.5100903908733088 1102 30-31 0.693239641907482 1102 32-33 0.6969349465429318 1102 34-35 0.5323727080736305 1102 36-37 0.7675270044574702 1102 38-39 0.6659475467445546 1102 40-41 0.8828089479509273 1102 42-43 0.8065815012992061 1102 44-45 0.9366878192624171 1102 46-47 0.8901481049610851 1102 48-49 0.8595930372954399 1102 50-51 1.030737752173657 1102 52-53 0.9607367575857424 1102 54-55 0.7549132484871492 1102 56-57 0.9344204568990477 1102 58-59 0.9263233318361515 1102 60-61 0.808769456656961 1102 62-63 0.7560627323091005 1102 64-65 0.9293581212710045 1102 66-67 0.6228284757897136 1102 68-69 0.7489838900837462 1102 70-71 0.8667367189885731 1102 72-73 0.9029599662565744 1102 74-75 1.023563232498983 1102 76-77 1.0363106000068747 1102 78-79 0.9393940209875034 1102 80-81 1.17112505373235 1102 82-83 1.4308143121000612 1102 84-85 1.3277676892449222 1102 86-87 1.446610684392681 1102 88-89 1.4402256407387384 1102 90-91 1.5813043377911775 1102 92-93 1.259194585276365 1102 94-95 1.2374142087351068 1102 96-97 1.5417770171054244 1102 98-99 1.626929777176688 1102 100-101 1.4772979470899603 1103 1 0.29286879492877915 1103 2 0.30541368926398604 1103 3 0.2631155929189646 1103 4 0.26987568601524003 1103 5 0.3177677056227566 1103 6 0.3484904804731528 1103 7 0.26375000833775886 1103 8 0.45330215680762365 1103 9 0.45476609783504784 1103 10-11 0.5074522683760563 1103 12-13 0.6410689566429966 1103 14-15 0.7031823816345124 1103 16-17 0.7507975573179593 1103 18-19 0.6608472962931344 1103 20-21 0.5371821571206823 1103 22-23 0.6313022280191021 1103 24-25 0.6618960057505703 1103 26-27 0.639950036996801 1103 28-29 0.6486007366567179 1103 30-31 0.7188171453110144 1103 32-33 0.7262104280143973 1103 34-35 0.6390180609264675 1103 36-37 0.6912708040882265 1103 38-39 0.807883102828626 1103 40-41 0.8537819945601441 1103 42-43 0.7326126005319225 1103 44-45 0.8062567036037507 1103 46-47 0.6088908152318027 1103 48-49 0.6567192656059078 1103 50-51 0.8629500712956926 1103 52-53 0.9084786311595181 1103 54-55 0.888228245210513 1103 56-57 0.8257405388234815 1103 58-59 0.8065675880285355 1103 60-61 0.82145800577198 1103 62-63 0.8573133290331505 1103 64-65 0.9759341799892205 1103 66-67 1.0571656600963166 1103 68-69 1.1867837136188264 1103 70-71 1.1772322038478897 1103 72-73 1.0323245357055555 1103 74-75 1.2297233353436994 1103 76-77 1.3260745225699146 1103 78-79 1.3431539813598938 1103 80-81 1.3005439485965589 1103 82-83 1.315677155398184 1103 84-85 1.461475725356923 1103 86-87 1.5721470231049395 1103 88-89 1.489411101717689 1103 90-91 1.612803953093092 1103 92-93 1.7012404889689527 1103 94-95 1.5021543621466549 1103 96-97 1.666897573899579 1103 98-99 1.7248532630375344 1103 100-101 1.8420124777269287 1104 1 0.33971732757527917 1104 2 0.2510989513658686 1104 3 0.3238907253258567 1104 4 0.3638484564703717 1104 5 0.3435765821522665 1104 6 0.4966477301096077 1104 7 0.3904645113323397 1104 8 0.5245840503534112 1104 9 0.5355193872781285 1104 10-11 0.6274220516375877 1104 12-13 0.556285788148962 1104 14-15 0.8375541679248357 1104 16-17 0.8622637176905243 1104 18-19 0.8850072917614469 1104 20-21 0.7819454693699797 1104 22-23 0.8265759163603406 1104 24-25 0.7997103438419941 1104 26-27 0.5890474754427188 1104 28-29 0.9082960489300973 1104 30-31 0.8526640655954196 1104 32-33 0.8675410673274655 1104 34-35 0.7912006059300367 1104 36-37 0.9278391053558437 1104 38-39 0.9722793865145931 1104 40-41 0.9071792431457695 1104 42-43 1.039009083437108 1104 44-45 0.9274318588880135 1104 46-47 0.8963943111458974 1104 48-49 0.9342287572521144 1104 50-51 1.0075361523308501 1104 52-53 0.9931720415461669 1104 54-55 1.0326538288981837 1104 56-57 1.1726717693359845 1104 58-59 1.183645228244103 1104 60-61 1.2352210569565472 1104 62-63 1.1388697346150103 1104 64-65 1.171640171288157 1104 66-67 1.2327461323443671 1104 68-69 1.3631367760395712 1104 70-71 1.3203177783517752 1104 72-73 1.3715354059596692 1104 74-75 1.2790121665344216 1104 76-77 1.2907389722843057 1104 78-79 1.2523984780659383 1104 80-81 1.3825640451084205 1104 82-83 1.5332096913438455 1104 84-85 1.5829101666373333 1104 86-87 1.8499709417797163 1104 88-89 1.7041434173181393 1104 90-91 1.60218513114636 1104 92-93 1.7922832592544218 1104 94-95 1.5978797417440909 1104 96-97 1.6190276323025543 1104 98-99 1.757626191992351 1104 100-101 1.3197450100603714 1105 1 0.39876798959562265 1105 2 0.3260133613220013 1105 3 0.31468457435038744 1105 4 0.24224887229182457 1105 5 0.31374952829693115 1105 6 0.42018325765282327 1105 7 0.3651130988279121 1105 8 0.3966726147615205 1105 9 0.510214829345216 1105 10-11 0.5531130181920503 1105 12-13 0.5579121180496855 1105 14-15 0.6397552129105009 1105 16-17 0.5720723103298013 1105 18-19 0.5849668990017136 1105 20-21 0.6162073341639029 1105 22-23 0.6695068422954975 1105 24-25 0.6320431904799051 1105 26-27 0.5905985033534975 1105 28-29 0.4951312144187696 1105 30-31 0.6358106178133625 1105 32-33 0.634256146093982 1105 34-35 0.5462630557367518 1105 36-37 0.6841766688589104 1105 38-39 0.7066988577298616 1105 40-41 0.7594028851644836 1105 42-43 0.7950181500541404 1105 44-45 0.8102851323813596 1105 46-47 0.7583634465979543 1105 48-49 0.7716343835851021 1105 50-51 0.8378539949507129 1105 52-53 0.6759236637891703 1105 54-55 0.7033376314658923 1105 56-57 0.7080303657789742 1105 58-59 0.7840966668918412 1105 60-61 0.8634954223956868 1105 62-63 0.916410793829531 1105 64-65 1.0131810997217912 1105 66-67 1.1665094078599303 1105 68-69 1.0426339363822201 1105 70-71 1.1163122659886646 1105 72-73 1.1965055844408283 1105 74-75 1.226542512270953 1105 76-77 1.3311168003406415 1105 78-79 1.652868426843849 1105 80-81 1.7317776670710607 1105 82-83 1.7493065793893514 1105 84-85 1.7372273069535176 1105 86-87 1.7751133622997557 1105 88-89 1.6260286084288005 1105 90-91 1.548965224492509 1105 92-93 1.5454773186611277 1105 94-95 1.588389052223718 1105 96-97 1.9551849833407395 1105 98-99 1.8355705094531807 1105 100-101 1.7649887497296763 1106 1 0.20329865707482853 1106 2 0.12991769448008128 1106 3 0.17317869835860833 1106 4 0.1814814982358044 1106 5 0.2939809135214091 1106 6 0.051178865346479085 1106 7 0.30890183397114157 1106 8 0.19479107260493578 1106 9 0.1142179420973477 1106 10-11 0.06871810295131553 1106 12-13 0.062082369459687925 1106 14-15 0.46652242913823017 1106 16-17 0.2931375815355466 1106 18-19 0.06983050698613624 1106 20-21 0.24504879739639307 1106 22-23 0.19829936382387103 1106 24-25 0.06038298879826698 1106 26-27 0.1810820926972454 1106 28-29 0.26628306713362093 1106 30-31 0.11830543238254876 1106 32-33 0.1317531115091768 1106 34-35 -0.20886231817188872 1106 36-37 -0.03532662512191109 1106 38-39 0.15516095305216737 1106 40-41 0.35384020991036635 1106 42-43 -0.16037594268715338 1106 44-45 0.07158771608511927 1106 46-47 0.10457123624522069 1106 48-49 0.27708823449562914 1106 50-51 0.09623644424651445 1106 52-53 0.08496428689721824 1106 54-55 0.3050064912984496 1106 56-57 -0.5619882475028959 1106 58-59 -0.23674463253778555 1106 60-61 0.2787017418002975 1106 62-63 0.01668658257264255 1106 64-65 0.20304099351380955 1106 66-67 -0.19498830786195498 1106 68-69 -0.15465222620978736 1106 70-71 -0.25961578422960585 1106 72-73 0.08720414636129448 1106 74-75 0.5120357654852761 1106 76-77 0.6084684542018088 1106 78-79 0.8042253766654106 1106 80-81 0.9978503243417158 1106 82-83 0.6331079955621206 1106 84-85 0.701090106877043 1106 86-87 0.4718448854372781 1106 88-89 0.6736743282452586 1106 90-91 0.7536676360290109 1106 92-93 0.6771888170240956 1106 94-95 0.7424993463120799 1106 96-97 0.7496419619398189 1106 98-99 0.8241919366589947 1106 100-101 0.29699745816657064 1107 1 0.18792442626344297 1107 2 0.041929514149387614 1107 3 0.21340097527433954 1107 4 0.03594827241925458 1107 5 0.17222231935550525 1107 6 0.32372962103025316 1107 7 0.2876028446810608 1107 8 0.15081251789663952 1107 9 0.2994111102149688 1107 10-11 0.44840124813028837 1107 12-13 0.3998168447673649 1107 14-15 0.391947036421449 1107 16-17 0.44816982189035315 1107 18-19 0.4716610364345115 1107 20-21 0.6102314283150321 1107 22-23 0.4581738593496496 1107 24-25 0.5493492056624802 1107 26-27 0.4378153677379615 1107 28-29 0.5896822300293678 1107 30-31 0.5947136175867271 1107 32-33 0.5397074245435007 1107 34-35 0.4704575297323146 1107 36-37 0.4050764257972048 1107 38-39 0.5062728024712655 1107 40-41 0.5518331267205596 1107 42-43 0.7026485441289978 1107 44-45 0.6415882225165532 1107 46-47 0.6700386308683335 1107 48-49 0.5495770318408546 1107 50-51 0.5856289075681573 1107 52-53 0.6580817320166901 1107 54-55 0.6389310486724824 1107 56-57 0.5343481614431767 1107 58-59 0.512908939002628 1107 60-61 0.40171730123333305 1107 62-63 0.7172195466106253 1107 64-65 0.728822206815881 1107 66-67 0.6829032443724081 1107 68-69 0.8789532304298966 1107 70-71 0.9615112630255886 1107 72-73 1.1027688394295936 1107 74-75 0.8543976662814714 1107 76-77 1.1622131232158388 1107 78-79 1.0699806543170531 1107 80-81 1.228360604165882 1107 82-83 0.9972770908162474 1107 84-85 1.3006902462313157 1107 86-87 1.3145106450942166 1107 88-89 1.143065808062378 1107 90-91 1.1030183517854333 1107 92-93 1.2768225349843583 1107 94-95 1.1828744456411435 1107 96-97 1.3759375823599171 1107 98-99 1.4511462237601158 1107 100-101 1.0230505297343822 1108 1 0.36503915577928936 1108 2 0.2808643185676729 1108 3 0.22674136326365257 1108 4 0.17069256089577323 1108 5 0.2133638230389039 1108 6 0.30243478514140776 1108 7 0.2878083061485981 1108 8 0.3732901700707316 1108 9 0.404972446868733 1108 10-11 0.43131615726925787 1108 12-13 0.2880003079688649 1108 14-15 0.4136635113300642 1108 16-17 0.5570042938226507 1108 18-19 0.6006690466778863 1108 20-21 0.5592775231642335 1108 22-23 0.3687908771387143 1108 24-25 0.5955992884277634 1108 26-27 0.5422585198664862 1108 28-29 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-2.234925830878826 2316 72-73 -1.9846545868563652 2316 74-75 -1.8485852433752328 2316 76-77 -2.14108776486464 2316 78-79 -2.2391575985024126 2316 80-81 -2.5009912229759514 2316 82-83 -2.68091601527529 2316 84-85 -2.6167752646100446 2316 86-87 -2.6641825263132013 2316 88-89 -2.7656301290308782 2316 90-91 -2.4250318569451643 2316 92-93 -2.583019922238048 2316 94-95 -3.090088129865528 2316 96-97 -3.3355624217357978 2316 98-99 -3.143221164945281 2316 100-101 -2.8718066311069546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10511.0 3 3707.0 4 3808.0 5 4553.0 6 5327.0 7 6516.0 8 8080.0 9 10015.0 10 12328.0 11 15106.0 12 18167.0 13 20764.0 14 23595.0 15 26848.0 16 30654.0 17 34952.0 18 34248.0 19 33513.0 20 36148.0 21 39712.0 22 43993.0 23 49007.0 24 54954.0 25 61033.0 26 68601.0 27 77960.0 28 88130.0 29 100664.0 30 116413.0 31 134600.0 32 157840.0 33 187116.0 34 225323.0 35 274050.0 36 324075.0 37 311172.0 38 133944.0 39 5319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78792919275254 18.209736730623995 14.099033173753348 23.90330090287011 2 15.699858234327365 19.621372743353948 39.66012083238494 25.01864818993374 3 19.487432077150462 23.485084755241147 29.018319004919054 28.009164162689338 4 13.240871291274434 16.025989175717672 35.93838110407393 34.79475842893397 5 14.621496912484409 36.751836047865275 33.46240719342 15.164259846230319 6 33.328626092641365 34.81341405892701 17.149648677360943 14.708311171070685 7 29.950920856254303 31.63163887377638 19.942113615907616 18.4753266540617 8 27.01076743059085 34.62586912537862 19.41812250651945 18.94524093751108 9 26.93748280881106 16.58641724161643 19.553580638136932 36.92251931143558 10-11 25.70017044014398 26.151393480283847 26.777696075577623 21.370740003994552 12-13 26.400970454135493 23.146663857909864 27.261170137398004 23.191195550556635 14-15 23.185078981554675 24.746067398023126 25.22368526146847 26.84516835895373 16-17 23.208810306653273 27.97631978463154 25.848048250158094 22.966821658557098 18-19 23.234378545629152 26.533426680486055 27.90411821775713 22.328076556127662 20-21 23.85902979846122 25.82467023312131 27.81166425214714 22.50463571627034 22-23 23.52129785621836 25.677186096536524 27.61323924809415 23.188276799150962 24-25 23.364623253656138 25.903253512839136 27.760676442639674 22.971446790865052 26-27 22.975915670631935 26.281756164392394 27.607839919803695 23.134488245171976 28-29 22.933727853260944 26.482741275787593 27.777989750435335 22.80554112051613 30-31 23.00554638463796 26.098751373696754 27.682188827899402 23.213513413765885 32-33 22.82219158398133 26.212032111280703 27.516523782083652 23.449252522654316 34-35 23.513646952769342 26.169695302014382 27.38347833377403 22.933179411442246 36-37 23.472231004622838 25.847097619114578 27.369909160819077 23.310762215443503 38-39 23.0343156718007 26.23003637182902 27.459683218186022 23.27596473818426 40-41 23.306966066427172 26.014491429832297 27.513836457035968 23.16470604670456 42-43 23.23054169864988 25.92420595960569 27.372074005965263 23.47317833577916 44-45 23.037100207569512 26.20621913560629 27.323366726247635 23.433313930576567 46-47 23.267200328892358 26.371229367680808 26.909421741775812 23.452148561651022 48-49 23.250120993104957 26.32854726448153 26.94023612052865 23.481095621884865 50-51 23.35198303478771 26.43362417986792 26.850753687147677 23.363639098196696 52-53 23.62372582126672 26.328879116629096 26.7647274739166 23.282667588187582 54-55 23.06370743547661 26.32671748727008 27.009633520827865 23.599941556425446 56-57 23.103100403746527 26.51705769986957 26.999770085798826 23.38007181058508 58-59 23.22989928422392 26.07402226333655 26.99562067950379 23.700457772935742 60-61 23.197847459751795 26.25216238964202 27.178471015920202 23.371519134685983 62-63 23.0458048002693 26.384775362831352 27.084466225416953 23.4849536114824 64-65 22.91629401757883 26.163860316887355 27.123535791232715 23.796309874301098 66-67 22.842265035535878 26.385557248010223 26.993641268566293 23.778536447887603 68-69 23.05682485568177 26.10813482115999 27.116625603414935 23.718414719743308 70-71 22.942991076825265 26.28157474110257 26.942579169110502 23.832855012961662 72-73 23.11096855925743 26.19202271947031 26.91862527720157 23.778383444070695 74-75 22.956096600016117 26.453307305891094 26.78483483617934 23.805761257913446 76-77 23.15554864718506 26.226189998653343 26.81592447845782 23.80233687570377 78-79 23.241546301972623 26.355732099989115 26.69747162525281 23.705249972785452 80-81 23.051169795862243 26.59074404574336 26.686191911263386 23.671894247131014 82-83 23.291510882872906 26.48045781009017 26.527725606146817 23.700305700890105 84-85 23.241720308126858 26.28846930619892 26.612645634057813 23.857164751616406 86-87 23.39302910312024 26.266114831751114 26.55785510644858 23.783000958680063 88-89 23.274591974059998 26.276125463118355 26.52456072039956 23.924721842422088 90-91 23.166484881373904 26.35406760263705 26.598931496495933 23.880516019493115 92-93 23.428432558742603 26.03904974558781 26.595959205222485 23.936558490447098 94-95 23.286473090228952 25.975909760655952 26.631346543570462 24.10627060554463 96-97 23.524887565916046 25.938371667524322 26.457586168418008 24.079154598141624 98-99 23.46053911776204 25.960552996580077 26.164007514977033 24.414900370680844 100-101 24.004702114321006 25.51416609586372 26.402325328475225 24.07880646134005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 103.5 2 108.0 3 148.5 4 135.5 5 136.0 6 240.0 7 478.0 8 822.0 9 1035.0 10 1032.0 11 1038.0 12 1136.5 13 1320.0 14 1506.0 15 1638.0 16 1798.0 17 2010.0 18 2219.0 19 2446.0 20 2741.0 21 3195.5 22 3845.0 23 4579.0 24 5549.5 25 7031.5 26 9176.0 27 12463.0 28 15882.0 29 19101.5 30 22956.5 31 27199.0 32 32681.0 33 37426.5 34 41289.5 35 46097.0 36 51911.5 37 57877.0 38 63048.5 39 67435.0 40 72990.5 41 81148.5 42 89260.5 43 96535.0 44 101766.5 45 106048.5 46 108694.0 47 111952.5 48 118967.5 49 121106.0 50 118809.0 51 115579.0 52 111305.0 53 108965.0 54 106193.0 55 98726.5 56 88931.0 57 81346.0 58 74239.0 59 65116.5 60 54413.5 61 44925.0 62 36906.0 63 28551.0 64 22063.0 65 17557.0 66 13478.5 67 10614.0 68 8224.5 69 6057.0 70 4789.0 71 3845.0 72 3139.0 73 2597.5 74 2027.0 75 1692.0 76 1239.0 77 753.5 78 465.5 79 298.0 80 186.5 81 106.0 82 76.5 83 51.0 84 34.0 85 24.0 86 14.0 87 6.5 88 8.0 89 6.5 90 4.0 91 2.0 92 1.5 93 1.5 94 1.5 95 2.0 96 1.5 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007412059671735274 2 0.10495046810558496 3 0.11400965214881696 4 0.11733970794336471 5 0.15826716786990297 6 0.09732356612452403 7 0.025960112376850596 8 0.025602041861307833 9 0.022128757860542993 10-11 0.0516874789185984 12-13 0.052833304568335245 14-15 0.027392394439021667 16-17 0.03149230184198634 18-19 0.03639786790492225 20-21 0.05892050333256228 22-23 0.03152810889354062 24-25 0.049360020567570405 26-27 0.057112247229071315 28-29 0.05390751611496355 30-31 0.06871373193265697 32-33 0.04978970518622173 34-35 0.0615881286733559 36-37 0.05351363854786651 38-39 0.031778758254420565 40-41 0.036773841946242156 42-43 0.032835066275271725 44-45 0.006713822166426879 46-47 0.012281818683116903 48-49 0.008289332434815054 50-51 0.007447866723289551 52-53 0.009345640455666215 54-55 0.011064378930271497 56-57 0.014322820621710674 58-59 0.019049351426875197 60-61 0.024921707881776573 62-63 0.01068840488895159 64-65 0.008575788847249266 66-67 0.01025872027030027 68-69 0.010956957775608666 70-71 0.030740353759346535 72-73 0.027911596686558675 74-75 0.029397589326061162 76-77 0.022988127097845635 78-79 0.004368460289621756 80-81 0.004368460289621756 82-83 0.005263636578478673 84-85 0.004654916702055969 86-87 0.01306957381731099 88-89 0.038420966317738885 90-91 0.0354847880902882 92-93 0.029361782274506882 94-95 0.04744434330941661 96-97 0.04373831347354897 98-99 0.02583478769641063 100-101 0.0747114130679983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.60556883241359 #Duplication Level Percentage of deduplicated Percentage of total 1 74.46297200537188 36.93778083278561 2 13.197470923608615 13.09336104629688 3 4.879111816274655 7.260933511297647 4 2.3334768248298476 4.630137810117556 5 1.3098115374750126 3.24869731898531 6 0.813039746733863 2.4198779472056855 7 0.5484791614620304 1.9045334557934464 8 0.3899244673748689 1.5473940004645013 9 0.298652720752683 1.333335428665633 >10 1.5704246491989573 14.21411214770223 >50 0.11879670713918743 4.077485539242163 >100 0.07188838212308231 6.573150618501643 >500 0.004354432430793451 1.395710477100597 >1k 0.0015240513507449255 1.1743062769675823 >5k 7.257387384499646E-5 0.18918358887349843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5255 0.188166055917724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4325 0.1548654979722467 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.580705155427669E-5 0.0 20-21 0.0 0.0 0.0 3.580705155427669E-5 0.0 22-23 0.0 7.161410310855338E-5 0.0 2.148423093256601E-4 0.0 24-25 0.0 8.951762888569171E-5 0.0 4.8339519598273526E-4 0.0 26-27 0.0 1.0742115466283006E-4 0.0 0.0010025974435197472 0.0 28-29 0.0 1.0742115466283006E-4 0.0 0.0036881263100904988 0.0 30-31 0.0 1.0742115466283006E-4 0.0 0.011100185981825774 0.0 32-33 0.0 1.0742115466283006E-4 0.0 0.027714657903010156 0.0 34-35 0.0 1.0742115466283006E-4 0.0 0.05458785009449481 0.0 36-37 0.0 1.7903525777138344E-4 0.0 0.10230074629056848 0.0 38-39 0.0 1.9693878354852176E-4 0.0 0.17412969170844753 0.0 40-41 0.0 2.148423093256601E-4 0.0 0.2529231086536334 0.0 42-43 0.0 2.148423093256601E-4 0.0 0.3364609599297609 0.0 44-45 0.0 2.148423093256601E-4 0.0 0.4387796097461065 0.0 46-47 0.0 2.148423093256601E-4 0.0 0.5605593920822015 0.0 48-49 0.0 2.148423093256601E-4 0.0 0.6928306405236997 0.0 50-51 0.0 2.148423093256601E-4 0.0 0.8364169172563491 0.0 52-53 0.0 2.148423093256601E-4 0.0 0.9771923404419879 0.0 54-55 0.0 2.148423093256601E-4 0.0 1.1342241650332685 1.7903525777138344E-5 56-57 0.0 2.148423093256601E-4 0.0 1.318988551053336 3.580705155427669E-5 58-59 0.0 2.148423093256601E-4 0.0 1.5189709339839714 3.580705155427669E-5 60-61 0.0 2.148423093256601E-4 0.0 1.7348337442789283 3.580705155427669E-5 62-63 0.0 2.148423093256601E-4 0.0 1.9519856084298393 3.580705155427669E-5 64-65 0.0 2.148423093256601E-4 0.0 2.184892575264632 3.580705155427669E-5 66-67 0.0 2.148423093256601E-4 0.0 2.4458364634664234 3.580705155427669E-5 68-69 0.0 2.148423093256601E-4 0.0 2.73227497237486 3.580705155427669E-5 70-71 0.0 2.148423093256601E-4 0.0 3.04277581992777 7.161410310855338E-5 72-73 0.0 2.148423093256601E-4 0.0 3.3573228643063135 7.161410310855338E-5 74-75 0.0 2.148423093256601E-4 0.0 3.677760168665536 1.0742115466283006E-4 76-77 0.0 2.148423093256601E-4 0.0 4.030978828722698 1.0742115466283006E-4 78-79 0.0 2.148423093256601E-4 0.0 4.41748014319956 1.0742115466283006E-4 80-81 0.0 2.148423093256601E-4 0.0 4.832824037703393 1.0742115466283006E-4 82-83 0.0 2.3274583510279846E-4 0.0 5.255562088353184 1.0742115466283006E-4 84-85 0.0 2.6855288665707515E-4 0.0 5.679947263374471 1.0742115466283006E-4 86-87 0.0 2.864564124342135E-4 0.0 6.125673441122107 1.0742115466283006E-4 88-89 0.0 2.864564124342135E-4 0.0 6.604001939309912 1.0742115466283006E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4880 0.0 44.539867 1 GTATCAA 8480 0.0 38.475193 1 ATCAACG 10415 0.0 30.458149 3 TCAACGC 10540 0.0 30.096931 4 TATCAAC 10660 0.0 29.981203 2 CAACGCA 10645 0.0 29.666027 5 AACGCAG 10980 0.0 28.824795 6 GTACATG 11475 0.0 25.656092 1 TACATGG 11545 0.0 25.211254 2 ACGCAGA 12645 0.0 24.615519 7 CGCAGAG 12815 0.0 24.288977 8 GTTCGCG 120 1.7065214E-4 23.760553 7 ACATGGG 12100 0.0 23.425985 3 GCAGAGT 13905 0.0 21.496037 9 GAGTACT 6320 0.0 20.975079 12-13 CATGGGG 6870 0.0 20.214504 4 GTACTTT 6585 0.0 20.019161 14-15 AGTACTT 6495 0.0 19.09316 12-13 AGAGTAC 12655 0.0 19.00025 10-11 TGGTATC 2865 0.0 18.42618 2 >>END_MODULE