FastQCFastQC Report
Thu 26 May 2016
SRR938367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792746
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.17864138020428638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44810.16045139801471384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27950.10008070909420334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.045.3996661
GTATCAA79750.036.771961
ATCAACG99150.029.1799533
TCAACGC100600.028.7593694
TATCAAC102000.028.6574332
CAACGCA102800.028.0976815
AACGCAG105000.027.541886
GTACATG113600.025.5637871
GGACCGA8250.024.7502776
TACATGG115250.024.7029152
ACGCAGA122400.023.3938377
ACATGGG119350.023.0868973
CGCAGAG123750.023.0235168
GAGTACT59550.020.81250412-13
GCAGAGT139800.019.870759
GTACTTT63700.019.30749514-15
AGAGTAC124750.019.18472510-11
AGGACCG11150.018.7472615
CATGGGG64650.018.5913964
TAAGGTG11000.018.5710225
Produced by FastQC (version 0.11.5)