FastQCFastQC Report
Thu 26 May 2016
SRR938366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758271
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.1869286955487695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42800.15516966969525475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49800.041.1042441
GTATCAA84300.036.310391
ATCAACG103750.029.228183
TCAACGC104250.029.133594
TATCAAC105000.028.9254932
CAACGCA106650.028.476435
GTACATG112650.027.6372051
AACGCAG110100.027.621276
TACATGG115800.026.3509142
ACATGGG121550.024.4787123
ACGCAGA126500.023.8097697
CGCAGAG128550.023.4304968
ATACCGT3101.0913936E-1121.4593876
GCAGAGT138650.021.1400499
GAGTACT60500.021.0026312-13
CATGGGG64850.020.2287164
AGTACTT63600.019.7175112-13
GTACTTT64650.019.58220114-15
CATGGGA60600.019.5297164
AGAGTAC125950.018.83970510-11
Produced by FastQC (version 0.11.5)