##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938366_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2758271 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.036315503444005 31.0 31.0 34.0 28.0 34.0 2 31.12919869004895 31.0 31.0 34.0 27.0 34.0 3 31.200313529743813 31.0 31.0 34.0 27.0 34.0 4 34.826264714380855 37.0 35.0 37.0 32.0 37.0 5 34.84369411127478 37.0 35.0 37.0 32.0 37.0 6 34.87522328299141 37.0 35.0 37.0 32.0 37.0 7 34.79468043567873 37.0 35.0 37.0 32.0 37.0 8 34.75465282417863 37.0 35.0 37.0 31.0 37.0 9 36.2541037483264 39.0 35.0 39.0 32.0 39.0 10-11 36.10052438647254 39.0 35.0 39.0 30.5 39.0 12-13 35.94919117809671 38.0 35.0 39.0 30.0 39.0 14-15 36.94157118716761 39.0 36.0 41.0 31.0 41.0 16-17 36.79815580122475 39.0 36.0 41.0 30.0 41.0 18-19 36.76863821575182 39.0 36.0 41.0 30.0 41.0 20-21 36.84895827857379 39.0 36.0 41.0 30.0 41.0 22-23 36.76463008892165 39.0 36.0 41.0 30.0 41.0 24-25 36.64671872343218 39.0 36.0 41.0 30.0 41.0 26-27 36.46852919818248 39.0 36.0 41.0 30.0 41.0 28-29 36.286214625031405 39.0 35.0 40.5 29.0 41.0 30-31 36.08727985756295 39.0 35.0 40.0 28.5 41.0 32-33 35.84330020509225 38.0 35.0 40.0 27.0 41.0 34-35 35.602332221888275 38.0 34.5 40.0 27.0 41.0 36-37 35.326685267691246 38.0 34.0 40.0 26.0 41.0 38-39 35.08360926101895 38.0 34.0 40.0 25.0 41.0 40-41 35.060611339494926 38.0 34.0 40.0 25.0 41.0 42-43 34.90574367058204 38.0 34.0 40.0 25.0 41.0 44-45 34.639946364951086 38.0 33.0 40.0 24.0 41.0 46-47 34.309238649864355 38.0 33.0 40.0 23.5 41.0 48-49 33.97719187128459 38.0 33.0 40.0 22.5 41.0 50-51 33.25056094923232 37.5 32.0 39.5 21.5 40.5 52-53 33.321196684444715 37.0 32.5 39.5 22.0 40.5 54-55 33.45636016185502 37.5 32.5 40.0 20.5 41.0 56-57 33.16967223307645 37.0 32.0 40.0 18.5 41.0 58-59 32.845661829457654 37.0 31.5 40.0 18.0 41.0 60-61 32.399331501509465 36.0 31.0 39.0 14.0 41.0 62-63 31.880930118904196 36.0 30.5 39.0 10.5 40.5 64-65 31.387560178097075 35.0 30.0 39.0 8.0 40.0 66-67 30.850002229657637 35.0 29.0 38.0 7.0 40.0 68-69 30.29499730084535 34.5 29.0 38.0 2.0 40.0 70-71 29.673418420452524 34.0 28.0 37.0 2.0 39.0 72-73 29.12616218638415 34.0 27.5 36.5 2.0 39.0 74-75 28.516018549301357 34.0 26.0 36.0 2.0 39.0 76-77 27.785233938217093 33.0 26.0 35.5 2.0 37.5 78-79 27.015205539992262 32.5 24.5 35.0 2.0 37.0 80-81 26.360648210418773 32.0 23.5 35.0 2.0 36.5 82-83 25.709459476606902 31.5 21.0 35.0 2.0 36.0 84-85 25.04837885762494 31.0 19.0 34.0 2.0 35.5 86-87 24.380508659228916 31.0 15.5 34.0 2.0 35.0 88-89 23.59995174513309 30.5 7.0 34.0 2.0 35.0 90-91 22.782738353120486 30.0 2.0 34.0 2.0 35.0 92-93 21.936411070558332 29.0 2.0 34.0 2.0 35.0 94-95 20.983011821536028 29.0 2.0 34.0 2.0 35.0 96-97 19.753205721990334 27.0 2.0 33.5 2.0 35.0 98-99 18.291165733896342 25.0 2.0 33.0 2.0 34.5 100-101 14.90805308832961 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6329356799645964 1101 2 0.7007757273236592 1101 3 0.6894206675768721 1101 4 0.4910598809654587 1101 5 0.6053577824392207 1101 6 0.648523638144276 1101 7 0.47776238300718177 1101 8 0.6374065166255747 1101 9 0.6009269976232687 1101 10-11 0.669941932731426 1101 12-13 0.8142204148388217 1101 14-15 0.9701701897375372 1101 16-17 0.9004665989130487 1101 18-19 0.8443931977966557 1101 20-21 0.9483057502665346 1101 22-23 1.0522499154593845 1101 24-25 1.0111737326867356 1101 26-27 1.0347066942138738 1101 28-29 0.9896457850633809 1101 30-31 0.7418420822859346 1101 32-33 0.875172616247994 1101 34-35 1.0259748818777084 1101 36-37 0.9392655864537929 1101 38-39 0.7725886974320844 1101 40-41 0.8639849217631905 1101 42-43 0.8986637157403266 1101 44-45 0.9445681390979459 1101 46-47 1.0296262781325112 1101 48-49 1.0156144886663157 1101 50-51 0.8922245053408417 1101 52-53 0.8762988826440861 1101 54-55 0.9105774406392513 1101 56-57 0.8627698096170704 1101 58-59 0.8926328366728526 1101 60-61 0.9591117751432492 1101 62-63 0.8768151660289369 1101 64-65 0.8711653419506753 1101 66-67 0.8709668542917761 1101 68-69 0.9343068022988916 1101 70-71 1.0812095174173564 1101 72-73 1.0993061172370666 1101 74-75 0.8227326417223431 1101 76-77 1.1237816221682202 1101 78-79 0.9344221327306883 1101 80-81 1.2903370107369305 1101 82-83 1.1230887871322075 1101 84-85 1.3952800896548219 1101 86-87 1.4574922189260562 1101 88-89 1.5183505570590015 1101 90-91 1.5066639601467848 1101 92-93 1.65656341638908 1101 94-95 1.3704505076330769 1101 96-97 1.2237634608540127 1101 98-99 1.4225477409185494 1101 100-101 1.5654853305089311 1102 1 0.5226758628456913 1102 2 0.5455072113690704 1102 3 0.5721699805511271 1102 4 0.590043866265475 1102 5 0.6052724617147405 1102 6 0.5441137201529997 1102 7 0.4268847042272981 1102 8 0.4451108229176626 1102 9 0.6430470469851883 1102 10-11 0.7082939862073587 1102 12-13 0.6281726802204091 1102 14-15 0.6581854978267927 1102 16-17 0.6617268015265054 1102 18-19 0.6939781900363542 1102 20-21 0.5783190990817033 1102 22-23 0.6496757892017726 1102 24-25 0.4224609664569883 1102 26-27 0.45384009937174596 1102 28-29 0.3991388791263759 1102 30-31 0.3334687063853181 1102 32-33 0.3636435370059843 1102 34-35 0.35477078151881614 1102 36-37 0.4831119843291489 1102 38-39 0.5429628598346525 1102 40-41 0.5329057619706603 1102 42-43 0.6486148114523402 1102 44-45 0.6553687344814776 1102 46-47 0.45329680672542594 1102 48-49 0.3467543813018139 1102 50-51 0.5741770003127371 1102 52-53 0.5136711439457784 1102 54-55 0.527796326304049 1102 56-57 0.4007483134483678 1102 58-59 0.40636745664270535 1102 60-61 0.5646191503442424 1102 62-63 0.3531022097666714 1102 64-65 0.4172256574977453 1102 66-67 0.34361054796555024 1102 68-69 0.32865275301608676 1102 70-71 0.4720172952545987 1102 72-73 0.5814474037029491 1102 74-75 0.6109154111244699 1102 76-77 0.389341291216887 1102 78-79 0.5530114243592479 1102 80-81 0.6134942188256467 1102 82-83 0.8174866283627473 1102 84-85 0.45999376493810473 1102 86-87 0.5144548942116671 1102 88-89 0.7346712481878939 1102 90-91 0.5946621265268526 1102 92-93 0.7060495141526673 1102 94-95 0.46845237383001503 1102 96-97 0.5887614092784084 1102 98-99 0.8013688877376772 1102 100-101 0.5863249950278444 1103 1 0.6071614436055128 1103 2 0.5828291058361934 1103 3 0.5151032968911551 1103 4 0.5708672660480687 1103 5 0.4783495073395443 1103 6 0.5153053238789838 1103 7 0.5030710802886702 1103 8 0.5157051168717288 1103 9 0.4260024151039161 1103 10-11 0.42689669184796486 1103 12-13 0.5130852918964379 1103 14-15 0.7718046941372663 1103 16-17 0.7527756022016945 1103 18-19 0.621482597057657 1103 20-21 0.5708531344757048 1103 22-23 0.7280702329834483 1103 24-25 0.7922461024022098 1103 26-27 0.6464367481830919 1103 28-29 0.5802373333530042 1103 30-31 0.6676929780134415 1103 32-33 0.7825360427356642 1103 34-35 0.9944252295537623 1103 36-37 0.8640129654702875 1103 38-39 0.7551249789394561 1103 40-41 0.8417270662414893 1103 42-43 0.7753173962379449 1103 44-45 0.7290398295345639 1103 46-47 0.6617190893957314 1103 48-49 0.792549908851349 1103 50-51 0.9924334426405963 1103 52-53 0.9346617548971281 1103 54-55 0.8895191511546869 1103 56-57 0.8862829097696547 1103 58-59 0.6492928444198256 1103 60-61 0.9062958265128316 1103 62-63 1.144265715258502 1103 64-65 0.9393772471746082 1103 66-67 1.1524457827955068 1103 68-69 0.9981836562572397 1103 70-71 0.9143650094202727 1103 72-73 0.8792637144611213 1103 74-75 0.7624243203839214 1103 76-77 0.7789262717029892 1103 78-79 0.9772738642374001 1103 80-81 0.8040944258574676 1103 82-83 0.7169098439359267 1103 84-85 0.9167152226673814 1103 86-87 0.9894483549997375 1103 88-89 1.036448876450926 1103 90-91 1.1340253665970685 1103 92-93 1.3101814069081108 1103 94-95 1.1395552813896401 1103 96-97 0.9156442762467556 1103 98-99 0.9398682108458836 1103 100-101 0.8039242558190267 1104 1 0.5617584806777387 1104 2 0.5387488835118184 1104 3 0.5897940589445554 1104 4 0.539031091949866 1104 5 0.5226690720800633 1104 6 0.5086770898571515 1104 7 0.43696912013995615 1104 8 0.5215509121717901 1104 9 0.4839450763177311 1104 10-11 0.6233613961769535 1104 12-13 0.5803433751171738 1104 14-15 0.6468583121478915 1104 16-17 0.7255785212570132 1104 18-19 0.7706497189825612 1104 20-21 0.8099515974027796 1104 22-23 0.8567197158054256 1104 24-25 0.7819344682370186 1104 26-27 0.8396265971584427 1104 28-29 0.7722640731963182 1104 30-31 0.8017166970844016 1104 32-33 0.8391380091718403 1104 34-35 0.7206733241058956 1104 36-37 0.6396305681059857 1104 38-39 0.6931285865408441 1104 40-41 0.710379567041393 1104 42-43 0.722232491856154 1104 44-45 0.7002976416961815 1104 46-47 0.8089957834803627 1104 48-49 0.8711284831641635 1104 50-51 0.6610918117309055 1104 52-53 0.7713220130180716 1104 54-55 0.905750702716702 1104 56-57 1.0210500154869138 1104 58-59 0.7412628574558155 1104 60-61 0.9697360977955185 1104 62-63 1.125642486050058 1104 64-65 1.0704495654706854 1104 66-67 1.1495135238250533 1104 68-69 1.0247366923290642 1104 70-71 0.9347103722832166 1104 72-73 0.8363607131100181 1104 74-75 0.7733196398634306 1104 76-77 0.76995812673945 1104 78-79 0.9732849636895544 1104 80-81 0.9133382407492334 1104 82-83 0.909024817717242 1104 84-85 0.8301246854253925 1104 86-87 1.0601145875992088 1104 88-89 1.0594746508207464 1104 90-91 0.9389599002286566 1104 92-93 1.1161945882061453 1104 94-95 1.2160895948206196 1104 96-97 0.9672718186486478 1104 98-99 0.9091432394537975 1104 100-101 0.7823081984998002 1105 1 0.5353719273953601 1105 2 0.5964367260489922 1105 3 0.4652626192457099 1105 4 0.481897950424063 1105 5 0.3539900202787436 1105 6 0.5183527438491069 1105 7 0.47896514053212513 1105 8 0.5124464301982385 1105 9 0.62352942248517 1105 10-11 0.6372500415428917 1105 12-13 0.5933583929563397 1105 14-15 0.7367083197192414 1105 16-17 0.7247450086022269 1105 18-19 0.6784899183348827 1105 20-21 0.7038003404258433 1105 22-23 0.7233081451886392 1105 24-25 0.7838003411074155 1105 26-27 0.815516762107606 1105 28-29 0.8877063064523512 1105 30-31 0.7141865777227352 1105 32-33 0.7567647761522522 1105 34-35 0.8174531093983433 1105 36-37 0.9279211432363539 1105 38-39 0.8502752767982429 1105 40-41 0.8411903125138593 1105 42-43 0.9318979178840578 1105 44-45 0.881520959328931 1105 46-47 0.799485902204438 1105 48-49 0.8352736246155814 1105 50-51 0.9295782590143844 1105 52-53 0.9146747662000294 1105 54-55 0.8941428933340703 1105 56-57 1.0500723283882252 1105 58-59 1.1806035921158653 1105 60-61 1.1838934596715092 1105 62-63 1.1642793508204399 1105 64-65 1.1358391977687354 1105 66-67 1.0975989020870465 1105 68-69 0.9613692746376046 1105 70-71 1.054352712335124 1105 72-73 1.0902011143650014 1105 74-75 1.0912011761301983 1105 76-77 0.9484617357496212 1105 78-79 1.3273069445141132 1105 80-81 1.3173348509097202 1105 82-83 1.4198293998451383 1105 84-85 1.3255252895394243 1105 86-87 1.6031692686859742 1105 88-89 1.7347814233269148 1105 90-91 1.688803824153858 1105 92-93 1.707352314038264 1105 94-95 1.3733377677343128 1105 96-97 1.2896583081722888 1105 98-99 1.519154423798902 1105 100-101 1.352557442028953 1106 1 0.36214539716826266 1106 2 0.4374323590197129 1106 3 0.28007510414983017 1106 4 0.5362240897698101 1106 5 0.49653628648035664 1106 6 0.12670174583186622 1106 7 0.35748126700899263 1106 8 0.4467219794164379 1106 9 0.4715571142504089 1106 10-11 0.22401746908072795 1106 12-13 0.33369625330615094 1106 14-15 0.48786628533441245 1106 16-17 0.4451572785332516 1106 18-19 0.12120382161096899 1106 20-21 0.38192337213670413 1106 22-23 0.40868826623526644 1106 24-25 0.6001416357455938 1106 26-27 0.37382200060115167 1106 28-29 0.3244146707540807 1106 30-31 0.3611152117815237 1106 32-33 0.6178589954108276 1106 34-35 0.42863994260702754 1106 36-37 0.23311342688279524 1106 38-39 0.31599646570506934 1106 40-41 0.5496637317276978 1106 42-43 0.10752813931100746 1106 44-45 0.5093227740187061 1106 46-47 0.18699295989225106 1106 48-49 0.4003581265932823 1106 50-51 0.19150157699360904 1106 52-53 0.5724336018038585 1106 54-55 0.29934853513515947 1106 56-57 -0.19703275757382954 1106 58-59 -0.5148675663079629 1106 60-61 0.2898651761089468 1106 62-63 0.3772720201447477 1106 64-65 0.4308167646119294 1106 66-67 -0.10140148856131148 1106 68-69 -0.2967487166997884 1106 70-71 -0.16346889106281282 1106 72-73 0.5174978044162053 1106 74-75 0.6329650194887471 1106 76-77 0.6116263524831957 1106 78-79 0.8091173317219713 1106 80-81 0.8000457486010113 1106 82-83 0.6179772373385255 1106 84-85 0.4960712575124724 1106 86-87 0.7065283785979588 1106 88-89 0.31259539238415 1106 90-91 0.5174796660088425 1106 92-93 0.639233103380942 1106 94-95 0.18775824516408335 1106 96-97 0.43216418925520017 1106 98-99 0.35317197423952607 1106 100-101 -0.1262027037613258 1107 1 0.5093540505791587 1107 2 0.49375174669285116 1107 3 0.5065961810367554 1107 4 0.5242054635265632 1107 5 0.47489375090304975 1107 6 0.46657625966304295 1107 7 0.5266015801388733 1107 8 0.4908909938815782 1107 9 0.6028893200399139 1107 10-11 0.5969253760756246 1107 12-13 0.5829710819787977 1107 14-15 0.6944500302362613 1107 16-17 0.6732240363342896 1107 18-19 0.6934501411412697 1107 20-21 0.6905869924832402 1107 22-23 0.7850157764278265 1107 24-25 0.8444630037520184 1107 26-27 0.7840970627399244 1107 28-29 0.8136112319706257 1107 30-31 0.8705794107637317 1107 32-33 0.7188374136815412 1107 34-35 0.8345855592616473 1107 36-37 0.8491708670580351 1107 38-39 0.8761834337907786 1107 40-41 0.9246859710657631 1107 42-43 0.8862071263832902 1107 44-45 0.9482708176701138 1107 46-47 0.8452507170109058 1107 48-49 0.907298676807649 1107 50-51 0.8349644502485276 1107 52-53 0.7933366653149037 1107 54-55 0.7468083335435338 1107 56-57 0.7500111558290072 1107 58-59 0.7284917861125351 1107 60-61 0.729521500451213 1107 62-63 0.7636682264327312 1107 64-65 0.7352280733810197 1107 66-67 0.6807781767638623 1107 68-69 0.8314331977074758 1107 70-71 0.8974702733686541 1107 72-73 0.7911360625336599 1107 74-75 0.6827627447328908 1107 76-77 0.6860558806188699 1107 78-79 0.6346381826497165 1107 80-81 0.4639626196443807 1107 82-83 0.6211419795986792 1107 84-85 0.6429319638113284 1107 86-87 0.529799021395533 1107 88-89 0.41965293840738127 1107 90-91 0.5045729676328214 1107 92-93 0.6417417382496744 1107 94-95 0.44637434651991015 1107 96-97 0.39469775470341517 1107 98-99 0.21796787154145392 1107 100-101 0.15011384628488145 1108 1 0.4096406626192035 1108 2 0.3648552937017371 1108 3 0.34323077740498675 1108 4 0.41416375997177823 1108 5 0.48432279557649593 1108 6 0.47936084091817577 1108 7 0.3729561512478341 1108 8 0.4194995704379352 1108 9 0.30810678519570445 1108 10-11 0.530488531041037 1108 12-13 0.5211681242971338 1108 14-15 0.6489322975774954 1108 16-17 0.6081693775049573 1108 18-19 0.6230155424351054 1108 20-21 0.5949626093229696 1108 22-23 0.6752969706558005 1108 24-25 0.7052464060187233 1108 26-27 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2316 60-61 -1.3024382637118812 2316 62-63 -1.332297025431668 2316 64-65 -1.4717936089295698 2316 66-67 -1.503880026842733 2316 68-69 -1.175065125571006 2316 70-71 -1.4130256060608204 2316 72-73 -1.2919876806101414 2316 74-75 -1.3170756105457642 2316 76-77 -1.2006616493402014 2316 78-79 -1.2569050586831096 2316 80-81 -1.2254110207591289 2316 82-83 -1.0536213067754971 2316 84-85 -1.1554621369782652 2316 86-87 -1.3977324308316064 2316 88-89 -1.4157899955265023 2316 90-91 -1.2978180728660327 2316 92-93 -1.3202255191051613 2316 94-95 -1.5143586574965475 2316 96-97 -1.3191681431919164 2316 98-99 -1.2397849791590971 2316 100-101 -0.8521265769503152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9061.0 3 3217.0 4 3476.0 5 3920.0 6 4614.0 7 5646.0 8 7239.0 9 8893.0 10 11169.0 11 13714.0 12 16362.0 13 18996.0 14 21470.0 15 24366.0 16 27701.0 17 31645.0 18 31298.0 19 30354.0 20 32694.0 21 35431.0 22 39535.0 23 44142.0 24 49489.0 25 55559.0 26 63171.0 27 71900.0 28 81937.0 29 94745.0 30 109311.0 31 127618.0 32 150716.0 33 181098.0 34 222748.0 35 275946.0 36 335223.0 37 341575.0 38 163989.0 39 8302.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.833710880139876 18.20647288473 14.044196402790687 23.915619832339434 2 15.691286487662886 19.610689445668633 39.67913526846033 25.01888879820815 3 19.443022804951344 23.52874821045713 29.035879359692768 27.992349624898754 4 13.30127902843172 16.003670949441016 35.95353999956471 34.74151002256256 5 14.597823362307045 36.76856981894968 33.46193205706809 15.171674761675186 6 33.31099842482665 34.84209449443901 17.167902160766356 14.679004919967989 7 29.95954706054304 31.62488512221111 19.968860593672456 18.446707223573394 8 27.055478004603735 34.62229905992289 19.40593190434069 18.916291031132687 9 26.885733456649074 16.569029613912917 19.627622726365995 36.91761420307201 10-11 25.678284399145994 26.147034144124586 26.794085788384734 21.380595668344686 12-13 26.377596994633873 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27.381516746687424 23.277262582821212 38-39 23.07060191124322 26.235723381605496 27.44271175106513 23.25096295608616 40-41 23.323161334073113 25.997019114655295 27.506645343545223 23.17317420772637 42-43 23.24063967508567 25.92582452450456 27.35218392471851 23.48135187569126 44-45 23.04479340317181 26.22740624775667 27.317638408599315 23.41016194047221 46-47 23.23824034152693 26.43249538518771 26.875956093745863 23.453308179539494 48-49 23.196131684721138 26.347749172270525 26.980092251748687 23.476026891259654 50-51 23.34019325994082 26.455006024190308 26.860909186826763 23.343891529042107 52-53 23.60594889890331 26.355418355829517 26.76703566286431 23.271597082402867 54-55 23.05762230054098 26.342604678820575 27.034438498020275 23.565334522618166 56-57 23.13451302951585 26.534661650277837 26.967452995828722 23.363372324377593 58-59 23.230913177248695 26.08351905269438 27.012934697066083 23.672633072990838 60-61 23.2165437832548 26.23106553324705 27.164271212141266 23.388119471356887 62-63 23.010177546457726 26.40391968688042 27.10338467819081 23.482518088471036 64-65 22.87928237385716 26.13713059401358 27.161697020061137 23.82189001206812 66-67 22.855740266021503 26.360576188369023 26.997468058874745 23.786215486734726 68-69 23.026340359048607 26.151962410592272 27.11029796472893 23.711399265630195 70-71 22.904098054350257 26.324047949431638 26.929825372554927 23.84202862366318 72-73 23.140717785520128 26.20194190529513 26.891319735548123 23.76602057363662 74-75 22.958104223881705 26.501379688097476 26.801087214890295 23.73942887313052 76-77 23.14017492654285 26.21729826728702 26.8721623568363 23.77036444933383 78-79 23.194271673833903 26.415503418410797 26.740394749674874 23.649830158080427 80-81 23.077822520485455 26.610669495167986 26.707746648028337 23.60376133631822 82-83 23.275377609548464 26.4941409790656 26.561650242554762 23.66883116883117 84-85 23.22782673767554 26.31514640413349 26.634200495766553 23.822826362424422 86-87 23.359371784963688 26.33085167654598 26.58235306167993 23.727423476810404 88-89 23.21078672254968 26.32196609375334 26.59350546723449 23.873741716462487 90-91 23.160769267455773 26.40146610032871 26.656013526538157 23.781751105677348 92-93 23.386077900225917 26.106457356498154 26.641119366992672 23.866345376283256 94-95 23.281647514928203 26.0059332007188 26.677534530248987 24.03488475410401 96-97 23.524914566840962 25.936192378518992 26.543531696631362 23.995361358008687 98-99 23.43247338463399 26.007605348656444 26.27705436973932 24.282866896970244 100-101 23.94353543491585 25.561112804078462 26.46121282729198 24.034138933713702 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 144.0 1 106.0 2 100.5 3 145.5 4 141.5 5 144.5 6 235.5 7 525.5 8 919.5 9 1149.5 10 1100.5 11 1053.5 12 1169.0 13 1333.5 14 1543.0 15 1699.5 16 1818.0 17 2000.5 18 2189.5 19 2445.0 20 2786.5 21 3200.0 22 3770.5 23 4444.5 24 5439.0 25 6937.0 26 9023.5 27 12021.0 28 15192.0 29 18642.5 30 22734.5 31 26978.5 32 32370.0 33 37206.0 34 40875.0 35 45499.0 36 51383.0 37 57600.0 38 62873.5 39 66786.5 40 71988.0 41 79870.0 42 87966.5 43 95911.0 44 101202.0 45 104820.0 46 107660.0 47 111054.0 48 117278.5 49 119129.0 50 117468.5 51 114412.0 52 110090.5 53 107848.5 54 105295.5 55 97825.5 56 87520.5 57 79570.0 58 72835.5 59 64200.0 60 53551.0 61 43968.0 62 36139.0 63 27982.0 64 21407.5 65 17241.5 66 13355.0 67 10282.5 68 7859.0 69 5940.5 70 4703.5 71 3689.5 72 3082.5 73 2567.5 74 1997.0 75 1657.5 76 1225.5 77 741.5 78 460.5 79 324.0 80 189.0 81 109.0 82 73.0 83 36.5 84 22.0 85 16.5 86 14.5 87 9.5 88 3.5 89 3.5 90 6.0 91 4.0 92 3.0 93 2.5 94 0.5 95 0.5 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1801853407442561 2 0.04879868584341423 3 0.046478391717130044 4 0.05405560222327682 5 0.05924000941169305 6 0.06340928791985993 7 0.03600081355312803 8 0.0342243383626917 9 0.04064140180569639 10-11 0.04167465778380732 12-13 0.03877429012595209 14-15 0.034876921085709126 16-17 0.03290104561879525 18-19 0.0406051472099732 20-21 0.05026699697020343 22-23 0.04743913850379459 24-25 0.06656343774777751 26-27 0.05271418218151878 28-29 0.04464753463310893 30-31 0.05271418218151878 32-33 0.049541905055739625 34-35 0.04881681314127582 36-37 0.04633337333423728 38-39 0.027064055707361603 40-41 0.025414471601956444 42-43 0.022514103944101214 44-45 0.0035166957851494648 46-47 0.01102139709984987 48-49 0.009951886526015754 50-51 0.00812102944199464 52-53 0.00899113973935121 54-55 0.011275179269912202 56-57 0.008864248654320043 58-59 0.010930760610541894 60-61 0.011039524397711465 62-63 0.003317295508671918 64-65 0.006435190740866289 66-67 0.004006132827412534 68-69 0.009426194888029493 70-71 0.023837396687997663 72-73 0.020556355775048933 74-75 0.021118302008758385 76-77 0.01343232771544203 78-79 0.004278042295336463 80-81 0.005637589634956101 82-83 0.004749352039737937 84-85 0.004278042295336463 86-87 0.008501702697088139 88-89 0.030598878790372663 90-91 0.03714283331840852 92-93 0.023239195858565022 94-95 0.032846663725210466 96-97 0.036200213829605574 98-99 0.021988412306114955 100-101 0.12364629871394073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2758271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.46072348924074 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14457298822683 36.67244222798515 2 13.505938859819617 13.36027014816259 3 4.901257108170262 7.2725916773065515 4 2.3289214633980797 4.607605621171613 5 1.2933447727234026 3.198488408996356 6 0.808678628043289 2.399869800798461 7 0.54652963037486 1.8922225648653528 8 0.40123363744894214 1.5876244797155517 9 0.2849845728329126 1.268598884002927 >10 1.5855647746397403 14.308554101231636 >50 0.12125983811112845 4.147311730917337 >100 0.07209570404092217 6.623435685894758 >500 0.004065673938899387 1.3296148457202484 >1k 0.0014784268868561084 1.1428559876576898 >5k 7.392134434280542E-5 0.18851383557371068 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5156 0.1869286955487695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4280 0.15516966969525475 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.6254595723190364E-5 0.0 16-17 0.0 0.0 0.0 3.6254595723190364E-5 0.0 18-19 0.0 0.0 0.0 3.6254595723190364E-5 0.0 20-21 0.0 0.0 0.0 3.6254595723190364E-5 0.0 22-23 0.0 0.0 0.0 1.0876378716957109E-4 0.0 24-25 0.0 1.8127297861595182E-5 0.0 4.894370422630699E-4 0.0 26-27 0.0 3.6254595723190364E-5 0.0 0.0010332559781109252 0.0 28-29 0.0 3.6254595723190364E-5 0.0 0.003969878231689344 0.0 30-31 0.0 3.6254595723190364E-5 0.0 0.012906636077455768 0.0 32-33 0.0 3.6254595723190364E-5 0.0 0.029184949557168237 0.0 34-35 0.0 3.6254595723190364E-5 0.0 0.05726413394477917 0.0 36-37 0.0 7.250919144638073E-5 3.6254595723190364E-5 0.10599031059674702 0.0 38-39 0.0 9.06364893079759E-5 3.6254595723190364E-5 0.18087417806299672 0.0 40-41 0.0 1.0876378716957108E-4 3.6254595723190364E-5 0.2639878387584106 0.0 42-43 0.0 1.0876378716957108E-4 3.6254595723190364E-5 0.34885984734639924 0.0 44-45 0.0 1.0876378716957108E-4 3.6254595723190364E-5 0.4551945766025166 0.0 46-47 0.0 1.0876378716957108E-4 3.6254595723190364E-5 0.5834452089733024 0.0 48-49 0.0 1.4501838289276146E-4 3.6254595723190364E-5 0.7162276658094872 0.0 50-51 0.0 1.4501838289276146E-4 3.6254595723190364E-5 0.8593789370225042 0.0 52-53 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.005249302914761 0.0 54-55 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.165277088436923 0.0 56-57 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.353384058346696 0.0 58-59 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.558222524182722 3.6254595723190364E-5 60-61 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.7786323388818577 3.6254595723190364E-5 62-63 0.0 1.4501838289276146E-4 3.6254595723190364E-5 1.995869876455214 3.6254595723190364E-5 64-65 0.0 1.4501838289276146E-4 3.6254595723190364E-5 2.2240381746391127 7.250919144638073E-5 66-67 0.0 1.4501838289276146E-4 3.6254595723190364E-5 2.487518449057399 7.250919144638073E-5 68-69 0.0 1.4501838289276146E-4 3.6254595723190364E-5 2.7770657778006584 7.250919144638073E-5 70-71 0.0 1.812729786159518E-4 3.6254595723190364E-5 3.0915562684014732 7.250919144638073E-5 72-73 0.0 1.812729786159518E-4 3.6254595723190364E-5 3.415944263634719 7.250919144638073E-5 74-75 0.0 1.812729786159518E-4 3.6254595723190364E-5 3.7437220635680832 7.250919144638073E-5 76-77 0.0 1.812729786159518E-4 3.6254595723190364E-5 4.096116733997493 7.250919144638073E-5 78-79 0.0 1.812729786159518E-4 3.6254595723190364E-5 4.4836964895762605 7.250919144638073E-5 80-81 0.0 2.1752757433914216E-4 3.6254595723190364E-5 4.899681720904146 7.250919144638073E-5 82-83 0.0 2.3565487220073732E-4 3.6254595723190364E-5 5.327522205033516 7.250919144638073E-5 84-85 0.0 2.537821700623325E-4 3.6254595723190364E-5 5.759169421713819 7.250919144638073E-5 86-87 0.0 2.537821700623325E-4 3.6254595723190364E-5 6.202853164174224 7.250919144638073E-5 88-89 0.0 2.537821700623325E-4 3.6254595723190364E-5 6.676537584595567 7.250919144638073E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4980 0.0 41.104244 1 GTATCAA 8430 0.0 36.31039 1 ATCAACG 10375 0.0 29.22818 3 TCAACGC 10425 0.0 29.13359 4 TATCAAC 10500 0.0 28.925493 2 CAACGCA 10665 0.0 28.47643 5 GTACATG 11265 0.0 27.637205 1 AACGCAG 11010 0.0 27.62127 6 TACATGG 11580 0.0 26.350914 2 ACATGGG 12155 0.0 24.478712 3 ACGCAGA 12650 0.0 23.809769 7 CGCAGAG 12855 0.0 23.430496 8 ATACCGT 310 1.0913936E-11 21.459387 6 GCAGAGT 13865 0.0 21.140049 9 GAGTACT 6050 0.0 21.00263 12-13 CATGGGG 6485 0.0 20.228716 4 AGTACTT 6360 0.0 19.71751 12-13 GTACTTT 6465 0.0 19.582201 14-15 CATGGGA 6060 0.0 19.529716 4 AGAGTAC 12595 0.0 18.839705 10-11 >>END_MODULE