FastQCFastQC Report
Thu 26 May 2016
SRR938366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758271
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.18642113120864484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45020.163218189945803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.10347061619398529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49300.046.7021261
GTATCAA84000.036.9238131
ATCAACG101750.030.1377813
TCAACGC103200.029.7604034
TATCAAC105550.029.249922
CAACGCA105450.029.0803155
AACGCAG108050.028.3957046
GTACATG112450.024.7476881
ACGCAGA125050.024.345537
TACATGG115950.023.8775882
CGCAGAG128200.023.5991488
TAAGGTG10450.023.2027725
ACATGGG119200.022.3356063
GCAGAGT141600.021.2317289
GAGTACT62800.020.91126812-13
AGTACTT65500.019.83174312-13
GTACTTT65950.019.588414-15
GTAAGGT8900.019.2308484
CATGGGG64800.019.0758674
AGAGTAC127000.019.05396310-11
Produced by FastQC (version 0.11.5)