FastQCFastQC Report
Thu 26 May 2016
SRR938365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742545
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.19252190939437638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43790.15966921235567694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.1014750897432859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.044.571071
GTATCAA80900.038.062321
ATCAACG100700.030.2953263
TATCAAC102300.029.91442
TCAACGC102550.029.7487984
CAACGCA105150.029.0132125
AACGCAG107950.028.1695466
GTACATG115100.025.63461
TACATGG116000.024.7837732
ACGCAGA123150.024.5320767
CGCAGAG125600.024.0157248
ACATGGG121850.022.7749463
GCAGAGT134250.021.5129979
CCTAGAC8850.020.940713
GAGTACT57650.020.88711212-13
AGTACTT60900.020.27943212-13
GTACTTT62800.019.25215514-15
AGAGTAC124250.019.22947110-11
CATGGGG68600.019.0492824
GAGTACA92450.018.9221461
Produced by FastQC (version 0.11.5)