##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742545 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.073609001857765 31.0 31.0 34.0 28.0 34.0 2 31.215959264114172 31.0 31.0 34.0 28.0 34.0 3 31.274960666096636 31.0 31.0 34.0 27.0 34.0 4 34.93452249644035 37.0 35.0 37.0 32.0 37.0 5 34.941812805259346 37.0 35.0 37.0 32.0 37.0 6 34.94516151968336 37.0 35.0 37.0 32.0 37.0 7 34.858657196144456 37.0 35.0 37.0 32.0 37.0 8 34.8162651843452 37.0 35.0 37.0 32.0 37.0 9 36.29463728033633 39.0 35.0 39.0 32.0 39.0 10-11 36.14711080401598 39.0 35.0 39.0 31.0 39.0 12-13 35.97755861799898 38.0 35.0 39.0 30.0 39.0 14-15 36.960994258982076 39.0 36.0 41.0 31.0 41.0 16-17 36.791668504983505 39.0 36.0 41.0 30.5 41.0 18-19 36.64625101867061 39.0 36.0 40.5 30.0 41.0 20-21 36.62307127139208 39.0 36.0 40.5 30.0 41.0 22-23 36.70759276511416 39.0 36.0 41.0 30.0 41.0 24-25 36.61712460506573 39.0 36.0 41.0 30.0 41.0 26-27 36.433454692630384 39.0 36.0 41.0 29.5 41.0 28-29 36.25233460162003 39.0 35.0 40.5 29.0 41.0 30-31 36.00700681301492 38.5 35.0 40.0 28.0 41.0 32-33 35.74265435936329 38.0 35.0 40.0 27.0 41.0 34-35 35.484210286431036 38.0 34.0 40.0 27.0 41.0 36-37 35.205541750454415 38.0 34.0 40.0 25.5 41.0 38-39 34.89978450672642 38.0 33.5 40.0 25.0 41.0 40-41 34.83025729751016 38.0 33.5 40.0 25.0 41.0 42-43 34.62583986771411 38.0 33.0 40.0 24.0 41.0 44-45 34.33944219693752 38.0 33.0 40.0 24.0 41.0 46-47 33.97372385867871 38.0 33.0 40.0 23.0 41.0 48-49 33.61630000601631 38.0 32.5 40.0 21.0 41.0 50-51 32.854833193256624 36.5 31.5 39.5 19.0 40.5 52-53 32.890969701499884 37.0 31.5 39.0 19.0 40.0 54-55 32.96400186687912 37.0 31.5 40.0 18.0 41.0 56-57 32.63936544341114 37.0 31.0 40.0 15.0 41.0 58-59 32.20641138067015 36.0 31.0 39.0 11.5 41.0 60-61 31.713592848977864 36.0 30.0 39.0 8.0 40.5 62-63 31.116299641391482 35.0 29.0 39.0 7.0 40.0 64-65 30.61596491579901 35.0 28.5 38.5 2.0 40.0 66-67 30.05410303203776 34.5 28.0 38.0 2.0 40.0 68-69 29.434035357669607 34.0 27.5 37.5 2.0 39.5 70-71 28.788861805366913 34.0 26.0 37.0 2.0 39.0 72-73 28.14402480178083 33.5 26.0 36.0 2.0 39.0 74-75 27.432523805443484 33.0 25.0 36.0 2.0 38.5 76-77 26.6840429236348 32.5 23.0 35.0 2.0 37.5 78-79 25.883732445593417 32.0 20.5 35.0 2.0 37.0 80-81 25.114189739821953 31.0 18.5 35.0 2.0 36.0 82-83 24.380989190696962 31.0 12.5 34.5 2.0 36.0 84-85 23.635948908769045 30.5 7.0 34.0 2.0 35.0 86-87 22.89136641331318 30.0 2.0 34.0 2.0 35.0 88-89 22.110150790597785 29.0 2.0 34.0 2.0 35.0 90-91 21.28373044015686 29.0 2.0 34.0 2.0 35.0 92-93 20.4030994933538 28.0 2.0 34.0 2.0 35.0 94-95 19.482119892289823 26.5 2.0 33.5 2.0 35.0 96-97 18.385800050682853 25.0 2.0 33.0 2.0 35.0 98-99 17.11325484176194 20.5 2.0 32.5 2.0 34.5 100-101 14.31678058154014 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6259612522171558 1101 2 0.5737048218272385 1101 3 0.5916728338767996 1101 4 0.43072141793874863 1101 5 0.5145238432940786 1101 6 0.46027420915202555 1101 7 0.4303238735561834 1101 8 0.32098448137784175 1101 9 0.6145075231750923 1101 10-11 0.6672209841042118 1101 12-13 0.7172944843320224 1101 14-15 0.8623160998929578 1101 16-17 0.8389057223180103 1101 18-19 0.9485648299920015 1101 20-21 0.937484132011356 1101 22-23 0.9533932041349473 1101 24-25 1.046681937144136 1101 26-27 1.0402686604509555 1101 28-29 1.13015884832906 1101 30-31 1.0498471449110482 1101 32-33 1.0887457388730226 1101 34-35 1.0982629920886566 1101 36-37 1.2523495952380017 1101 38-39 1.3113590563644948 1101 40-41 1.4570919893604852 1101 42-43 1.5489693177845112 1101 44-45 1.44096539681847 1101 46-47 1.6322419151845367 1101 48-49 1.63909287055111 1101 50-51 1.761906420711135 1101 52-53 1.9024965655027728 1101 54-55 2.013040041048619 1101 56-57 1.996026550133891 1101 58-59 2.2620034379572544 1101 60-61 2.4653458369775088 1101 62-63 2.374064976240721 1101 64-65 2.3058341943705862 1101 66-67 2.5813912226929148 1101 68-69 2.5608558102248153 1101 70-71 2.687858579254023 1101 72-73 2.767340996194637 1101 74-75 2.8881994820908368 1101 76-77 3.1005154588330157 1101 78-79 3.249223261132304 1101 80-81 3.6401683988864413 1101 82-83 3.7147110385216138 1101 84-85 3.9532262202249946 1101 86-87 4.080880681218336 1101 88-89 4.3916164014355665 1101 90-91 4.419204520927369 1101 92-93 4.550802412031274 1101 94-95 4.859557674539317 1101 96-97 4.868194865621334 1101 98-99 5.123416940752094 1101 100-101 5.057230184326093 1102 1 0.6200200069672306 1102 2 0.5492328901520374 1102 3 0.546283740934232 1102 4 0.5477629008511968 1102 5 0.5436495756632027 1102 6 0.5846403365238828 1102 7 0.6123828951999997 1102 8 0.6351965729351008 1102 9 0.7919998023205252 1102 10-11 0.6506924773111251 1102 12-13 0.7294775480179538 1102 14-15 1.0307319266916366 1102 16-17 1.0056836476640143 1102 18-19 1.0253827258526158 1102 20-21 0.8952939511590756 1102 22-23 0.7605430967419906 1102 24-25 0.7188148947080322 1102 26-27 0.7979286696015961 1102 28-29 0.7992482640859606 1102 30-31 0.931743488466843 1102 32-33 0.8413053769142849 1102 34-35 0.9003965533407623 1102 36-37 0.9656124665062578 1102 38-39 1.0864955216746637 1102 40-41 1.3654931052304917 1102 42-43 1.3875963083669802 1102 44-45 1.5351765034107459 1102 46-47 1.4279495330943774 1102 48-49 1.2287744091299686 1102 50-51 1.4240755042488686 1102 52-53 1.498431201955384 1102 54-55 1.615089721411593 1102 56-57 1.797139242837524 1102 58-59 1.713322655577329 1102 60-61 1.820752818155146 1102 62-63 2.11695702407825 1102 64-65 2.2250902598485247 1102 66-67 2.190880248276585 1102 68-69 2.33537921426381 1102 70-71 2.483730940501051 1102 72-73 2.6308283441605234 1102 74-75 2.656219257645912 1102 76-77 3.0367788511795197 1102 78-79 3.3932546910472787 1102 80-81 3.7264943841691576 1102 82-83 3.7670821034276294 1102 84-85 3.8881818332544924 1102 86-87 4.1965433720155865 1102 88-89 4.529569326463843 1102 90-91 4.612020790007094 1102 92-93 4.4466681009348505 1102 94-95 4.762580881585311 1102 96-97 5.006748937021765 1102 98-99 5.059686211115448 1102 100-101 4.876518817194691 1103 1 0.4721185755736137 1103 2 0.4631408704841249 1103 3 0.3974691349630355 1103 4 0.35140037958522896 1103 5 0.20492645290752876 1103 6 0.3833471246055993 1103 7 0.4410645634463819 1103 8 0.3959272030104941 1103 9 0.5378095214515355 1103 10-11 0.4282151974261339 1103 12-13 0.667965520785458 1103 14-15 0.7708897582184591 1103 16-17 0.8356833161484971 1103 18-19 0.8446956900685265 1103 20-21 0.8784227494605119 1103 22-23 0.6976477456444528 1103 24-25 0.767948132303701 1103 26-27 0.9029148137596152 1103 28-29 0.774386558800245 1103 30-31 0.8866724461971032 1103 32-33 0.9058285445752361 1103 34-35 1.0978111089818796 1103 36-37 1.109532697843818 1103 38-39 1.2606070977446677 1103 40-41 1.1486792406485549 1103 42-43 1.4218298068050927 1103 44-45 1.4746899893115426 1103 46-47 1.7108817577594309 1103 48-49 1.5810280365878882 1103 50-51 1.6443052595753471 1103 52-53 1.7973867773683736 1103 54-55 1.8744951935273662 1103 56-57 1.926264011718466 1103 58-59 2.107823189769384 1103 60-61 2.189764027900498 1103 62-63 2.2837311067973936 1103 64-65 2.4351654686656268 1103 66-67 2.3634108720230387 1103 68-69 2.446877528808752 1103 70-71 2.6208706245667592 1103 72-73 2.6747135403348388 1103 74-75 2.995820293782611 1103 76-77 3.124154890744393 1103 78-79 3.088828356056869 1103 80-81 3.647268050987016 1103 82-83 3.8007615188753476 1103 84-85 4.0905198464819605 1103 86-87 4.263841776672475 1103 88-89 4.350195468873874 1103 90-91 4.465529128375945 1103 92-93 4.526302348899421 1103 94-95 4.655230353646843 1103 96-97 4.751708318835728 1103 98-99 4.884057876182304 1103 100-101 4.929364350550427 1104 1 0.6274239432371438 1104 2 0.5044469051822773 1104 3 0.506268489489127 1104 4 0.5219463373161233 1104 5 0.4232734703700203 1104 6 0.4554119897921751 1104 7 0.5211693575050589 1104 8 0.43813946151223604 1104 9 0.691306792954272 1104 10-11 0.6736616193300193 1104 12-13 0.6640533599245515 1104 14-15 0.7363166042283567 1104 16-17 0.9022883092507499 1104 18-19 1.0051749976776065 1104 20-21 0.9722430218105202 1104 22-23 0.9107827830977726 1104 24-25 0.9285913911833177 1104 26-27 0.978435780151294 1104 28-29 0.9437900512991959 1104 30-31 1.0812491262624846 1104 32-33 1.1413747942741495 1104 34-35 1.179533798883213 1104 36-37 1.3455850488320422 1104 38-39 1.3023973361309373 1104 40-41 1.398664852801609 1104 42-43 1.48906498760347 1104 44-45 1.5070792085048481 1104 46-47 1.6716028654187056 1104 48-49 1.7110728368340347 1104 50-51 1.6760362172248264 1104 52-53 1.7494698428304147 1104 54-55 2.0152521318384586 1104 56-57 2.225853726471321 1104 58-59 2.3663233191898243 1104 60-61 2.3976426584806667 1104 62-63 2.4204904695659657 1104 64-65 2.5480116683369367 1104 66-67 2.71447953110939 1104 68-69 2.83387706334436 1104 70-71 2.858891022463016 1104 72-73 2.9457111641643685 1104 74-75 3.0479891529232006 1104 76-77 3.2802894194995886 1104 78-79 3.449027683182887 1104 80-81 3.807451179502916 1104 82-83 4.097230193795351 1104 84-85 4.171490087080453 1104 86-87 4.063644782483198 1104 88-89 4.685867096376587 1104 90-91 4.870490872143694 1104 92-93 4.898899789013694 1104 94-95 4.9725731976511405 1104 96-97 5.229523943279517 1104 98-99 5.603925443420572 1104 100-101 5.429621787733792 1105 1 0.5678740486601335 1105 2 0.4782231045533365 1105 3 0.4846230854965299 1105 4 0.38644692443116213 1105 5 0.2825694853434726 1105 6 0.4521219173786335 1105 7 0.40472084778934203 1105 8 0.4530690157611801 1105 9 0.5651276635382274 1105 10-11 0.4864372909149921 1105 12-13 0.6014793665167844 1105 14-15 0.7613402015817954 1105 16-17 0.7256197696504216 1105 18-19 0.8263495418513642 1105 20-21 0.7493038707675979 1105 22-23 0.7417657994462488 1105 24-25 0.7422496816396276 1105 26-27 0.9426769977210299 1105 28-29 0.9756142636334957 1105 30-31 1.009740084905637 1105 32-33 1.0629645337475537 1105 34-35 1.0334049729038952 1105 36-37 1.1500549352264926 1105 38-39 1.236782129770873 1105 40-41 1.1682179715448981 1105 42-43 1.3317992309337967 1105 44-45 1.3994987667645375 1105 46-47 1.342033676850889 1105 48-49 1.4333097048665735 1105 50-51 1.320522154651833 1105 52-53 1.5209212196648423 1105 54-55 1.5391528414771898 1105 56-57 1.6558563654017533 1105 58-59 1.6300493144531742 1105 60-61 2.0438904779402343 1105 62-63 2.207860999558765 1105 64-65 2.3054700696122232 1105 66-67 2.3778240895167357 1105 68-69 2.4242290209582507 1105 70-71 2.619459831432909 1105 72-73 2.7782637132423 1105 74-75 2.966962378114971 1105 76-77 3.034553855898686 1105 78-79 3.216567644321078 1105 80-81 3.608657100908424 1105 82-83 3.908500359599646 1105 84-85 4.047184987528613 1105 86-87 4.36861990459078 1105 88-89 4.612562615886148 1105 90-91 4.905276565758232 1105 92-93 5.085355726929301 1105 94-95 5.193957983774283 1105 96-97 5.48535160367306 1105 98-99 5.591952605343515 1105 100-101 5.2683264943722445 1106 1 0.0886747626162041 1106 2 0.19108442891234745 1106 3 0.20346376560922863 1106 4 0.22158112836381605 1106 5 0.2378815092234845 1106 6 0.10561694096995211 1106 7 0.28306791199270265 1106 8 0.1377151934199219 1106 9 0.08393301989460866 1106 10-11 0.06728812720031385 1106 12-13 0.11258145717739865 1106 14-15 0.42389375355539727 1106 16-17 0.2667071460304413 1106 18-19 0.4496469025530203 1106 20-21 0.3671778758546296 1106 22-23 0.2758624437684247 1106 24-25 0.30425993000698526 1106 26-27 0.48392189540067676 1106 28-29 0.3086781688130529 1106 30-31 0.6675560784277721 1106 32-33 0.6911081768411691 1106 34-35 0.6239684592559129 1106 36-37 0.6186912722820281 1106 38-39 0.6618413714770384 1106 40-41 0.7681585191078923 1106 42-43 0.6312219338096412 1106 44-45 0.7352310511500093 1106 46-47 1.0252218605407393 1106 48-49 1.1292043652632984 1106 50-51 0.6824574355244337 1106 52-53 1.0807591623839912 1106 54-55 0.9226274478846648 1106 56-57 1.0393553375620996 1106 58-59 0.42340184975585515 1106 60-61 1.6919346192015503 1106 62-63 1.9217934950965407 1106 64-65 2.1487239960545104 1106 66-67 2.2651152947706805 1106 68-69 1.7568317548747459 1106 70-71 2.0912956552535036 1106 72-73 2.5075216087998804 1106 74-75 2.799423066277587 1106 76-77 2.9686425846438453 1106 78-79 3.1894316370592932 1106 80-81 3.6916008236582947 1106 82-83 3.9347225852755123 1106 84-85 4.225776078039612 1106 86-87 4.488700063966508 1106 88-89 4.575674446525991 1106 90-91 4.640604233768659 1106 92-93 4.959437269752964 1106 94-95 4.847933671230951 1106 96-97 5.209576851755941 1106 98-99 5.531620828081795 1106 100-101 5.042599262258554 1107 1 0.46870921730027604 1107 2 0.3134716492091414 1107 3 0.35112261855452687 1107 4 0.35598332842838687 1107 5 0.3978850166169323 1107 6 0.40114783141136456 1107 7 0.44089262251853967 1107 8 0.3355061700284949 1107 9 0.390804313275126 1107 10-11 0.49946422373027843 1107 12-13 0.45503020659621995 1107 14-15 0.7161433784521094 1107 16-17 0.7726152766861816 1107 18-19 0.8285252751833667 1107 20-21 0.8801591653572274 1107 22-23 0.9230649802585162 1107 24-25 0.8780349182818341 1107 26-27 0.9560560098015998 1107 28-29 0.9509254334522339 1107 30-31 1.0400288897838053 1107 32-33 1.0777094136161125 1107 34-35 1.193604367960134 1107 36-37 1.1966833809005735 1107 38-39 1.2719792358957278 1107 40-41 1.3768528195681782 1107 42-43 1.369852091099233 1107 44-45 1.3720091168961304 1107 46-47 1.1561924238149288 1107 48-49 1.3402867006899015 1107 50-51 1.5734274328443973 1107 52-53 1.7839302571983282 1107 54-55 1.7671978277186327 1107 56-57 1.9171224857213005 1107 58-59 2.161857969776726 1107 60-61 2.1461000042229976 1107 62-63 2.32511671231536 1107 64-65 2.340161946153085 1107 66-67 2.550227234425236 1107 68-69 2.899143165222519 1107 70-71 2.8100087920915904 1107 72-73 3.016794502282732 1107 74-75 3.0534973022140086 1107 76-77 3.288779223251364 1107 78-79 3.440330248009669 1107 80-81 3.7195903689864025 1107 82-83 3.8923380069443994 1107 84-85 4.04031985802504 1107 86-87 4.241429397247057 1107 88-89 4.546386696110282 1107 90-91 4.693379782414329 1107 92-93 4.876625159956831 1107 94-95 4.857980310427269 1107 96-97 4.96654824035889 1107 98-99 5.150247942415717 1107 100-101 5.106302612910591 1108 1 0.3292433538596704 1108 2 0.30837253799909803 1108 3 0.3424814324583494 1108 4 0.37265860265500805 1108 5 0.29162277003281645 1108 6 0.3301294309701319 1108 7 0.39371917342357676 1108 8 0.39873792684427656 1108 9 0.40456507866447566 1108 10-11 0.40379859618433045 1108 12-13 0.4855554285744077 1108 14-15 0.4242295870942385 1108 16-17 0.5990804716245677 1108 18-19 0.7306532979263309 1108 20-21 0.6275700442165117 1108 22-23 0.5924162553287218 1108 24-25 0.6316386715434774 1108 26-27 0.6698462346105956 1108 28-29 0.7241251833030091 1108 30-31 0.8438622328029268 1108 32-33 0.873935391487926 1108 34-35 0.969029839100692 1108 36-37 1.0664053110261946 1108 38-39 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22-23 -0.18505982023002332 2311 24-25 -0.18533597035865057 2311 26-27 0.1090891711413775 2311 28-29 0.08030529935205521 2311 30-31 0.06688237568713618 2311 32-33 0.14153190544792693 2311 34-35 0.00988158470368461 2311 36-37 0.18628476368179037 2311 38-39 0.20184052716768974 2311 40-41 0.10804269255343968 2311 42-43 0.26712304127021014 2311 44-45 0.38041626724439936 2311 46-47 0.17720620714290902 2311 48-49 0.4419627214857087 2311 50-51 0.3922808162562177 2311 52-53 0.5076574675183707 2311 54-55 0.3803454236700716 2311 56-57 0.604677490385022 2311 58-59 0.5224905202956123 2311 60-61 0.609272279986353 2311 62-63 0.9001339098915828 2311 64-65 1.0169480474509882 2311 66-67 0.888554359549218 2311 68-69 0.812921653323361 2311 70-71 1.0636686818634011 2311 72-73 1.134673255544751 2311 74-75 1.2278979849213059 2311 76-77 1.3770672912829482 2311 78-79 1.776803807158835 2311 80-81 1.6824799034294244 2311 82-83 1.754027145229287 2311 84-85 1.9725973509262538 2311 86-87 2.151777622809501 2311 88-89 2.368811089942426 2311 90-91 2.5479359746271797 2311 92-93 2.666163304056326 2311 94-95 2.931149813937779 2311 96-97 2.901020581709691 2311 98-99 3.0231698648858725 2311 100-101 3.130066051130454 2312 1 -0.18795395074391763 2312 2 -0.2672796038100387 2312 3 -0.18645376380521483 2312 4 -0.2060354090489227 2312 5 -0.15684696259257436 2312 6 -0.05770539122148932 2312 7 -0.19156473975089483 2312 8 -0.3350612529960415 2312 9 -0.28120582996069743 2312 10-11 -0.23764510637582958 2312 12-13 -0.3516493237390961 2312 14-15 -0.21282645031386238 2312 16-17 -0.06602691623930923 2312 18-19 -0.10944000583494784 2312 20-21 -0.26944975511465685 2312 22-23 -0.22223245342343034 2312 24-25 -0.2798074193887743 2312 26-27 -0.14779699820599745 2312 28-29 -0.1767157265026711 2312 30-31 -0.08093355910422417 2312 32-33 0.01305892046266166 2312 34-35 -0.1873164620903509 2312 36-37 -0.032155318756529994 2312 38-39 0.1775279680604811 2312 40-41 0.22787981296371385 2312 42-43 0.199623337499645 2312 44-45 0.10956310207176045 2312 46-47 0.033538704820784915 2312 48-49 0.25025007823603573 2312 50-51 0.4076889627209255 2312 52-53 0.458221601523249 2312 54-55 0.5031708555548278 2312 56-57 0.44790067074858797 2312 58-59 0.5750683043752005 2312 60-61 0.7458108601531386 2312 62-63 0.8782448292856664 2312 64-65 0.7488299564200176 2312 66-67 0.8554277544183897 2312 68-69 1.2067054284712455 2312 70-71 1.1845322317082712 2312 72-73 1.0728082904554448 2312 74-75 1.2948547123471812 2312 76-77 1.4836187435616068 2312 78-79 1.5442699715446473 2312 80-81 1.7710428511852037 2312 82-83 1.9323705215169937 2312 84-85 2.419345062415207 2312 86-87 2.483369526493 2312 88-89 2.1999405095857014 2312 90-91 2.3932658233260575 2312 92-93 2.364091218010884 2312 94-95 2.5120409741408736 2312 96-97 2.6755461042606257 2312 98-99 2.9501194095801253 2312 100-101 2.88515032452071 2313 1 -0.46883872965270257 2313 2 -0.5206699015547791 2313 3 -0.48467346250837195 2313 4 -0.49906947219791675 2313 5 -0.37082220246853126 2313 6 -0.44212963443817443 2313 7 -0.35756119200561187 2313 8 -0.4859244359860355 2313 9 -0.5969887201527513 2313 10-11 -0.519303720881048 2313 12-13 -0.4216354639970774 2313 14-15 -0.4684585504352512 2313 16-17 -0.4572588860442508 2313 18-19 -0.256859881925358 2313 20-21 -0.32243918815095896 2313 22-23 -0.4457734758627012 2313 24-25 -0.33446318131500874 2313 26-27 -0.29645907546752426 2313 28-29 -0.312492111768627 2313 30-31 -0.2397586844629771 2313 32-33 -0.2961686495279494 2313 34-35 -0.16690167226375507 2313 36-37 -0.053873109565948596 2313 38-39 -0.05289743449311146 2313 40-41 0.09559227997599606 2313 42-43 0.1570815566971362 2313 44-45 0.12192654765592437 2313 46-47 0.16101411794351606 2313 48-49 0.24153930664502354 2313 50-51 0.2849748939931658 2313 52-53 0.33742201862303034 2313 54-55 0.3516237836432623 2313 56-57 0.4338774883687577 2313 58-59 0.3554483249904692 2313 60-61 0.5024356425050733 2313 62-63 0.4801053797963597 2313 64-65 0.6531318699796849 2313 66-67 0.8440131345697566 2313 68-69 1.004232575914056 2313 70-71 1.238435677684702 2313 72-73 1.2050478264591753 2313 74-75 1.4029467532377815 2313 76-77 1.4079690136740304 2313 78-79 1.5937272950889927 2313 80-81 1.6442258847689288 2313 82-83 1.6222397801297568 2313 84-85 1.9171421043627213 2313 86-87 2.0878752501741964 2313 88-89 1.986214952090652 2313 90-91 2.372729433876021 2313 92-93 2.5077769178515865 2313 94-95 2.820454556688059 2313 96-97 2.971239396450578 2313 98-99 3.0751359715360067 2313 100-101 2.8717940877457604 2314 1 -0.3556072331761406 2314 2 -0.3510731152030573 2314 3 -0.4909412438363354 2314 4 -0.3450176394749249 2314 5 -0.3871209628212213 2314 6 -0.21358029900390818 2314 7 -0.3312937024737934 2314 8 -0.3426194555120645 2314 9 -0.42971949443383295 2314 10-11 -0.41298001195743694 2314 12-13 -0.4629041936625313 2314 14-15 -0.43657933458877096 2314 16-17 -0.4659874815807399 2314 18-19 -0.27278903381985486 2314 20-21 -0.27067357827269234 2314 22-23 -0.36479372165232604 2314 24-25 -0.32894531609565547 2314 26-27 -0.39643573177542635 2314 28-29 -0.43046967264459823 2314 30-31 -0.4425818122259173 2314 32-33 -0.19468000282986253 2314 34-35 -0.23922334834085035 2314 36-37 0.03504593159188829 2314 38-39 0.11612208180613948 2314 40-41 -0.07298565148108338 2314 42-43 0.06997408331137223 2314 44-45 0.12375513687016593 2314 46-47 0.16331298528812255 2314 48-49 0.242694819403809 2314 50-51 0.21761326615970233 2314 52-53 0.22172727630334776 2314 54-55 0.14176885496309666 2314 56-57 0.3525028367004168 2314 58-59 0.6473636262943145 2314 60-61 0.47739710109676636 2314 62-63 0.5150664493265218 2314 64-65 0.6092218988188698 2314 66-67 0.6882990364387105 2314 68-69 0.8668821822324162 2314 70-71 0.9785741835911388 2314 72-73 0.9394496200328533 2314 74-75 1.061897357012647 2314 76-77 1.1319833505915966 2314 78-79 1.4781881773832843 2314 80-81 1.5414985634089646 2314 82-83 1.771564515203167 2314 84-85 1.956991245752544 2314 86-87 1.9630088684951055 2314 88-89 2.132397042615544 2314 90-91 2.310563722841753 2314 92-93 2.279369589877575 2314 94-95 2.45721941275961 2314 96-97 2.460622879815766 2314 98-99 2.595947845694056 2314 100-101 2.6841944515182927 2315 1 -0.6242321924462608 2315 2 -0.38183557854616623 2315 3 -0.5666271350060974 2315 4 -0.36136498388678717 2315 5 -0.33044452098348387 2315 6 -0.3793606834939709 2315 7 -0.4692083326464882 2315 8 -0.47677716160525563 2315 9 -0.4498400548769794 2315 10-11 -0.4645000510581241 2315 12-13 -0.4091358652167827 2315 14-15 -0.537827298094804 2315 16-17 -0.422282691746922 2315 18-19 -0.336844100616851 2315 20-21 -0.3477508021241036 2315 22-23 -0.3917634186220269 2315 24-25 -0.43245949009369866 2315 26-27 -0.5397201892534369 2315 28-29 -0.48948563875733697 2315 30-31 -0.4275182736139911 2315 32-33 -0.4226809803469749 2315 34-35 -0.5527929149749298 2315 36-37 -0.4052717614677377 2315 38-39 -0.41307798336167423 2315 40-41 -0.36265492486003126 2315 42-43 -0.2557965260076287 2315 44-45 -0.11354528672057995 2315 46-47 -0.051983528234202936 2315 48-49 -0.16707220568579828 2315 50-51 -0.00955193423130396 2315 52-53 0.0856943665607588 2315 54-55 0.21423545646195663 2315 56-57 0.4368625629956284 2315 58-59 0.6089129909082018 2315 60-61 0.704853927424562 2315 62-63 0.7754346474366542 2315 64-65 0.5724802891740346 2315 66-67 0.843383754587947 2315 68-69 0.9300037201926656 2315 70-71 1.1001545029133943 2315 72-73 1.1966979093779138 2315 74-75 1.3324936424476412 2315 76-77 1.387664354175822 2315 78-79 1.79077859771564 2315 80-81 1.902024793451318 2315 82-83 1.9773090573993244 2315 84-85 2.1388550450356973 2315 86-87 2.256637086155582 2315 88-89 2.3881432140068775 2315 90-91 2.4888204840017387 2315 92-93 2.5807609225592287 2315 94-95 2.5345654538153255 2315 96-97 2.509313006892988 2315 98-99 2.6900827430547594 2315 100-101 2.8195170321634553 2316 1 -1.0072527203054982 2316 2 -0.8933670220130949 2316 3 -0.8587374858261256 2316 4 -0.6272919959428336 2316 5 -0.6297950151726894 2316 6 -0.5229427406244156 2316 7 -0.6451635840856156 2316 8 -0.6188657898229124 2316 9 -0.6427096106497601 2316 10-11 -0.6617581155742513 2316 12-13 -0.553775270017475 2316 14-15 -1.2364544296251623 2316 16-17 -1.0001354125028712 2316 18-19 -0.8391027081980411 2316 20-21 -0.7097199560287422 2316 22-23 -0.8828180509669821 2316 24-25 -0.9338274709778105 2316 26-27 -0.8449640254650106 2316 28-29 -0.8656983030345202 2316 30-31 -0.6783209237714871 2316 32-33 -0.5282305621868773 2316 34-35 -0.672133090928007 2316 36-37 -0.674550028004063 2316 38-39 -0.436012163876498 2316 40-41 -0.5594959154107002 2316 42-43 -0.42989015042383016 2316 44-45 -0.3850913929441049 2316 46-47 -0.24200362164138767 2316 48-49 -0.1895903983652829 2316 50-51 -0.11428637541735753 2316 52-53 0.04291224426251006 2316 54-55 0.015441060893365943 2316 56-57 0.3052127313457973 2316 58-59 0.5010184541426952 2316 60-61 0.22772837078287367 2316 62-63 0.5322110132452771 2316 64-65 0.6991643339225959 2316 66-67 0.9437568402836121 2316 68-69 0.9840104541993995 2316 70-71 0.6876542313808649 2316 72-73 1.0477585154385203 2316 74-75 1.480578795048924 2316 76-77 1.5312013369063529 2316 78-79 1.534998213225581 2316 80-81 1.8983140299018473 2316 82-83 2.0369767015830966 2316 84-85 1.9675918757080133 2316 86-87 2.4136931304696922 2316 88-89 2.413759049784005 2316 90-91 2.538971456088195 2316 92-93 2.6602971449986796 2316 94-95 2.7112706781011973 2316 96-97 2.510123259961304 2316 98-99 2.6025569474644463 2316 100-101 2.6602181291548757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8430.0 3 3248.0 4 3624.0 5 3959.0 6 4773.0 7 5946.0 8 7385.0 9 9730.0 10 12031.0 11 14619.0 12 17477.0 13 20686.0 14 23414.0 15 27184.0 16 30905.0 17 35423.0 18 35321.0 19 34573.0 20 37295.0 21 41312.0 22 46188.0 23 52077.0 24 58244.0 25 64795.0 26 73018.0 27 81534.0 28 91694.0 29 103561.0 30 116504.0 31 132331.0 32 152025.0 33 176683.0 34 209283.0 35 247891.0 36 292073.0 37 298593.0 38 158565.0 39 10151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.780659474250704 18.211192827040794 14.07158549506285 23.936562203645654 2 15.698076071517935 19.562419891026263 39.70654856436695 25.032955473088858 3 19.508861396702763 23.501077010343312 28.987318453993144 28.002743138960778 4 13.247347710639179 16.02178665609265 35.95290720388894 34.77795842937923 5 14.583679165260538 36.789500086648 33.418400386723704 15.20842036136776 6 33.33488993830007 34.79942415345012 17.205130375394745 14.660555532855065 7 30.00689348292313 31.61548225201698 19.945545132252747 18.432079132807143 8 27.044906272317636 34.60224549598674 19.409475844249446 18.94337238744617 9 26.852341249909262 16.578739162264817 19.5981123947323 36.97080719309361 10-11 25.65646817151096 26.12264236622887 26.810997932288938 21.409891529971233 12-13 26.435106916518485 23.152345780331007 27.2150640587243 23.197483244426206 14-15 23.22563361793612 24.69045138467806 25.284008586410007 26.79990641097581 16-17 23.223800928064346 27.95692179652863 25.85469029540543 22.964586980001595 18-19 23.22712673216158 26.531329400331856 27.880313285797154 22.36123058170941 20-21 23.855796291442953 25.861869919455398 27.768400391825786 22.51393339727586 22-23 23.542008076014177 25.633576114664663 27.633193869932477 23.19122193938868 24-25 23.37007932040475 25.90327602063505 27.746527839845964 22.980116819114237 26-27 22.980175475458825 26.332608873285885 27.55410468994861 23.133110961306684 28-29 22.967567935280677 26.514269331547002 27.733465034433845 22.784697698738473 30-31 22.970369403091233 26.122299801791538 27.699596360901356 23.207734434215872 32-33 22.804612165064082 26.174483547847338 27.536376195100598 23.484528091987983 34-35 23.542160430740992 26.19619476134098 27.315776304722554 22.94586850319547 36-37 23.47967699108589 25.834348511153582 27.386804633587676 23.299169864172857 38-39 23.056619448539145 26.24380658680844 27.453094901361258 23.246479063291158 40-41 23.27994850549226 26.03479526192178 27.49775714431591 23.187499088270048 42-43 23.292249896654713 25.926127858589 27.30695218837635 23.47467005637994 44-45 23.074071724049407 26.206291972250913 27.32570393773375 23.393932365965938 46-47 23.24861733787465 26.41020960886129 26.900001404041852 23.441171649222206 48-49 23.19109015420466 26.341281519691773 26.95780842299887 23.5098199031047 50-51 23.31692713295039 26.441300960943277 26.867813550932002 23.373958355174334 52-53 23.632931685464303 26.33236344449527 26.74311522654978 23.291589643490646 54-55 23.06417573962501 26.333631435054393 27.054946437410948 23.547246387909652 56-57 23.14841150203362 26.52751128390367 26.969933656699492 23.354143557363216 58-59 23.25854586706506 26.101345962895312 26.94926867441015 23.690839495629476 60-61 23.260015023446787 26.236097842020435 27.167897957248815 23.335989177283963 62-63 23.0975393283747 26.37427791929907 27.120023832947666 23.408158919378565 64-65 22.924811751786393 26.173534960408574 27.11100641481576 23.790646872989274 66-67 22.90052286721353 26.400227240034503 26.98003208416341 23.719217808588557 68-69 23.10532522542759 26.136491264407386 27.10805063113891 23.650132879026117 70-71 23.00833643305691 26.310727231618642 26.913860505145543 23.767075830178907 72-73 23.14723933204032 26.20696772612508 26.872626310936425 23.773166630898178 74-75 23.055341973806538 26.504535783487242 26.744247742592346 23.695874500113867 76-77 23.23359566844401 26.256812726862982 26.80332597453081 23.706265630162193 78-79 23.287275365991736 26.432757707500155 26.661810782797048 23.61815614371107 80-81 23.164641154746 26.592574549146136 26.67536844285489 23.567415853252975 82-83 23.345307829394976 26.516501064649518 26.524067934813328 23.614123171142182 84-85 23.36707169104744 26.26840636625763 26.623456773241955 23.741065169452977 86-87 23.47179821422874 26.29794759367551 26.546515284798154 23.683738907297595 88-89 23.31965545832187 26.334240146723975 26.51915859825213 23.826945796702027 90-91 23.27225047346283 26.376871853649153 26.591496179015913 23.75938149387211 92-93 23.53062591647275 26.084514787735703 26.59885178568368 23.78600751010787 94-95 23.414513615803394 25.9950746596619 26.642517120617953 23.94789460391675 96-97 23.65517969503457 25.947352864108712 26.493568585676 23.903898855180717 98-99 23.572759494834145 26.02835290134139 26.191817229194857 24.20707037462961 100-101 24.058039096307386 25.616419930790947 26.390180862990643 23.935360109911027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 134.0 1 101.5 2 106.0 3 150.0 4 146.5 5 156.0 6 277.5 7 547.5 8 867.0 9 1109.0 10 1163.5 11 1116.0 12 1217.0 13 1419.0 14 1590.0 15 1770.5 16 1932.0 17 2065.5 18 2239.5 19 2509.0 20 2869.5 21 3255.0 22 3783.0 23 4569.0 24 5609.0 25 7047.5 26 9307.5 27 12532.5 28 15529.5 29 18792.5 30 22706.0 31 26747.5 32 32155.5 33 36963.0 34 40723.5 35 45177.5 36 50794.5 37 56941.5 38 61982.5 39 65971.5 40 71438.0 41 79801.0 42 87527.5 43 94203.5 44 99584.0 45 103588.0 46 106081.0 47 109394.5 48 115951.0 49 118102.5 50 116125.5 51 113002.5 52 108747.5 53 106731.5 54 104219.0 55 96404.5 56 86667.0 57 79495.0 58 72622.5 59 64094.0 60 54079.5 61 44697.5 62 36690.0 63 28596.0 64 22197.5 65 17586.0 66 13548.5 67 10766.0 68 8279.0 69 6147.5 70 4871.5 71 3869.0 72 3229.5 73 2652.5 74 2164.0 75 1813.5 76 1303.5 77 830.0 78 527.5 79 334.0 80 208.5 81 121.5 82 72.5 83 50.5 84 36.5 85 25.5 86 19.5 87 13.5 88 9.5 89 7.0 90 2.0 91 0.5 92 1.0 93 1.5 94 1.0 95 1.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3017635079825491 2 0.03525922090612916 3 0.042296480094219054 4 0.04499470382436751 5 0.0572460980585551 6 0.05604283612484025 7 0.030081548342871307 8 0.03205052241622289 9 0.03945240643271122 10-11 0.03157651013930492 12-13 0.0335454842126565 14-15 0.028094343028099815 16-17 0.021932183428166174 18-19 0.02565135667783026 20-21 0.03720996373806082 22-23 0.03084726048250803 24-25 0.05046407625034411 26-27 0.043499742027933906 28-29 0.035860851872986585 30-31 0.03598847056292604 32-33 0.03217814110616234 34-35 0.031029572896707255 36-37 0.030081548342871307 38-39 0.018924028593879042 40-41 0.018413553834121226 42-43 0.01908810976665834 44-45 0.009370858089839913 46-47 0.016827435830588013 48-49 0.014949617964336045 50-51 0.0068367155324707525 52-53 0.00490420394195902 54-55 0.007019027946669973 56-57 0.005214135046097694 58-59 0.007146646636609427 60-61 0.006016309668574262 62-63 0.004484885389300814 64-65 0.00807643994902545 66-67 0.002570605040209003 68-69 0.007328959050808646 70-71 0.013308806236543064 72-73 0.013618737340681739 74-75 0.010774663679173905 76-77 0.009152083192800848 78-79 0.006107465875673872 80-81 0.007037259188089894 82-83 0.011959694371468836 84-85 0.0042843417336816715 86-87 0.0011850306922949305 88-89 0.019161034732338027 90-91 0.028349580407978723 92-93 0.01363696858210166 94-95 0.02196864591100602 96-97 0.018103622729982553 98-99 0.01299887513240439 100-101 0.10481140692313162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2742545.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.11119186409282 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6003128538956 36.14599085824924 2 13.775758857330157 13.530878726316345 3 5.033751333630561 7.416405826261907 4 2.3658883483678457 4.647663864228598 5 1.354529981948035 3.326129091455806 6 0.8294851213068718 2.4442201764547256 7 0.5460647699423624 1.8772524180802748 8 0.39296894055612064 1.5439338429024738 9 0.3095461438058005 1.368196205331306 >10 1.593262964529861 14.30724874667392 >50 0.12045783319114618 4.125407413699368 >100 0.07213698056306427 6.556881133019968 >500 0.004264674912383639 1.364982195516678 >1k 0.0014963771622209898 1.1507988957940911 >5k 7.481885811104949E-5 0.19401060601528786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5280 0.19252190939437638 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4379 0.15966921235567694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 0.1014750897432859 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8231241419922006E-5 0.0 12-13 0.0 0.0 0.0 7.292496567968802E-5 0.0 14-15 0.0 0.0 0.0 7.292496567968802E-5 0.0 16-17 0.0 0.0 0.0 1.0938744851953204E-4 0.0 18-19 0.0 0.0 0.0 1.6408117277929806E-4 0.0 20-21 0.0 0.0 0.0 2.0054365561914206E-4 0.0 22-23 0.0 0.0 0.0 3.4639358697851816E-4 0.0 24-25 0.0 0.0 0.0 6.198622082773483E-4 0.0 26-27 0.0 0.0 0.0 0.0012214931751347744 0.0 28-29 0.0 0.0 0.0 0.0038650231810234657 0.0 30-31 0.0 0.0 0.0 0.012342550441287198 0.0 32-33 0.0 0.0 0.0 0.030355016964170138 0.0 34-35 0.0 0.0 0.0 0.05914214716622699 0.0 36-37 0.0 0.0 0.0 0.10887697375977422 0.0 38-39 0.0 5.469372425976602E-5 0.0 0.18229418295780014 0.0 40-41 0.0 7.292496567968802E-5 0.0 0.2647540879001074 0.0 42-43 0.0 7.292496567968802E-5 0.0 0.3456278748388814 0.0 44-45 0.0 7.292496567968802E-5 0.0 0.44907193865551887 0.0 46-47 0.0 7.292496567968802E-5 0.0 0.5733543114151272 0.0 48-49 0.0 7.292496567968802E-5 0.0 0.7060595177107395 0.0 50-51 0.0 7.292496567968802E-5 0.0 0.8487007505802093 0.0 52-53 0.0 7.292496567968802E-5 0.0 0.9901204902745442 0.0 54-55 0.0 7.292496567968802E-5 0.0 1.1452318922752407 0.0 56-57 0.0 7.292496567968802E-5 0.0 1.3315734108282635 0.0 58-59 0.0 7.292496567968802E-5 0.0 1.5288901367160794 0.0 60-61 0.0 7.292496567968802E-5 0.0 1.7447844976107958 0.0 62-63 0.0 7.292496567968802E-5 0.0 1.960988789609651 0.0 64-65 0.0 7.292496567968802E-5 0.0 2.193765280059215 0.0 66-67 0.0 7.292496567968802E-5 0.0 2.451773808633951 0.0 68-69 0.0 7.292496567968802E-5 0.0 2.741741703417811 0.0 70-71 0.0 1.0938744851953204E-4 0.0 3.051472263900866 0.0 72-73 0.0 1.0938744851953204E-4 0.0 3.3682036210891706 0.0 74-75 3.646248283984401E-5 1.0938744851953204E-4 0.0 3.6979156221684604 0.0 76-77 3.646248283984401E-5 1.0938744851953204E-4 0.0 4.053588911029719 0.0 78-79 3.646248283984401E-5 1.0938744851953204E-4 0.0 4.439161435819649 1.8231241419922006E-5 80-81 3.646248283984401E-5 1.4584993135937605E-4 0.0 4.851716197911065 3.646248283984401E-5 82-83 3.646248283984401E-5 1.6408117277929806E-4 0.0 5.27827255341298 3.646248283984401E-5 84-85 3.646248283984401E-5 2.0054365561914206E-4 0.0 5.7023676913232055 3.646248283984401E-5 86-87 3.646248283984401E-5 2.1877489703906407E-4 0.0 6.146553657278185 3.646248283984401E-5 88-89 3.646248283984401E-5 2.1877489703906407E-4 0.0 6.624230413721562 3.646248283984401E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 44.57107 1 GTATCAA 8090 0.0 38.06232 1 ATCAACG 10070 0.0 30.295326 3 TATCAAC 10230 0.0 29.9144 2 TCAACGC 10255 0.0 29.748798 4 CAACGCA 10515 0.0 29.013212 5 AACGCAG 10795 0.0 28.169546 6 GTACATG 11510 0.0 25.6346 1 TACATGG 11600 0.0 24.783773 2 ACGCAGA 12315 0.0 24.532076 7 CGCAGAG 12560 0.0 24.015724 8 ACATGGG 12185 0.0 22.774946 3 GCAGAGT 13425 0.0 21.512997 9 CCTAGAC 885 0.0 20.94071 3 GAGTACT 5765 0.0 20.887112 12-13 AGTACTT 6090 0.0 20.279432 12-13 GTACTTT 6280 0.0 19.252155 14-15 AGAGTAC 12425 0.0 19.229471 10-11 CATGGGG 6860 0.0 19.049282 4 GAGTACA 9245 0.0 18.922146 1 >>END_MODULE