FastQCFastQC Report
Thu 26 May 2016
SRR938365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742545
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51230.18679729958852087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44590.16258621098286444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29100.10610582506394607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48350.042.494661
GTATCAA79750.036.259351
TCAACGC99700.028.4687064
ATCAACG99850.028.3783263
TATCAAC102250.027.815382
CAACGCA102100.027.799515
AACGCAG105650.026.8379766
GTACATG109900.024.1914121
TACATGG111000.023.3518142
ACGCAGA123400.022.8236167
CGCAGAG124300.022.6583618
ACATGGG115100.022.057413
TAAGGTG11550.020.5814785
GGACCGA7900.020.4407486
GCAGAGT137500.020.3104529
CATGGGG61100.020.3089684
GAGTACT59300.019.90291212-13
GTGTAAG12000.019.8169061
AGTACTT63350.019.15531212-13
AGAGTAC124900.018.78492410-11
Produced by FastQC (version 0.11.5)