##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938365_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742545 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812006730974332 31.0 31.0 34.0 30.0 34.0 2 32.040980913713355 33.0 31.0 34.0 30.0 34.0 3 32.116620146615645 33.0 31.0 34.0 30.0 34.0 4 35.77826398472951 37.0 35.0 37.0 35.0 37.0 5 35.644799264916344 37.0 35.0 37.0 33.0 37.0 6 35.68164752082463 37.0 35.0 37.0 33.0 37.0 7 35.612830418461684 37.0 35.0 37.0 33.0 37.0 8 35.602196499966276 37.0 35.0 37.0 33.0 37.0 9 37.17734440091229 39.0 37.0 39.0 33.0 39.0 10-11 37.107705798810954 39.0 37.0 39.0 33.0 39.0 12-13 36.99527263909982 39.0 37.0 39.0 33.0 39.0 14-15 38.05669095675732 40.0 37.0 41.0 33.0 41.0 16-17 37.97121706298347 40.0 37.0 41.0 33.0 41.0 18-19 37.952114368223675 40.0 37.0 41.0 33.0 41.0 20-21 37.94161718403891 40.0 37.0 41.0 32.5 41.0 22-23 37.972619592385904 40.0 37.0 41.0 33.0 41.0 24-25 37.898007143000385 40.0 37.0 41.0 32.5 41.0 26-27 37.72937946323579 40.0 37.0 41.0 32.0 41.0 28-29 37.59902718095783 39.5 37.0 41.0 32.0 41.0 30-31 37.38549139576561 39.0 36.0 41.0 31.5 41.0 32-33 37.20191172797529 39.0 36.0 41.0 31.0 41.0 34-35 37.03286874053114 39.0 36.0 41.0 31.0 41.0 36-37 37.06168485840706 39.0 36.0 41.0 31.0 41.0 38-39 36.93024745263979 39.0 36.0 41.0 30.5 41.0 40-41 36.767809097024845 39.0 35.0 41.0 30.0 41.0 42-43 36.558397947891464 39.0 35.0 40.0 30.0 41.0 44-45 36.29457310636653 39.0 35.0 40.0 30.0 41.0 46-47 35.96593346690756 38.0 35.0 40.0 29.0 41.0 48-49 35.60696925665759 38.0 34.5 40.0 28.0 41.0 50-51 35.251345374460584 38.0 34.0 40.0 27.0 41.0 52-53 34.741386741147366 38.0 33.0 40.0 26.0 41.0 54-55 34.411113582457176 38.0 33.0 40.0 25.0 41.0 56-57 34.047731030849086 37.0 33.0 40.0 24.0 41.0 58-59 33.584574182009774 37.0 32.0 39.5 23.0 41.0 60-61 33.16381098578145 36.0 31.5 39.0 22.0 40.5 62-63 32.673234532159 36.0 31.0 39.0 21.5 40.0 64-65 32.1652432321074 35.0 31.0 38.5 19.5 40.0 66-67 31.66203289280577 35.0 30.0 38.0 18.0 40.0 68-69 31.085626489264534 34.5 29.5 37.5 15.5 39.5 70-71 30.506441826843314 34.0 29.0 37.0 11.5 39.0 72-73 29.911159342873134 34.0 28.5 36.0 8.0 39.0 74-75 29.279579733422786 33.5 27.5 36.0 7.0 38.5 76-77 27.980790470165484 31.5 26.0 34.5 3.5 36.5 78-79 28.603277612582474 33.0 27.0 35.0 2.0 37.0 80-81 28.27977863626668 33.0 26.5 35.0 2.0 36.5 82-83 27.77604615421078 33.0 26.0 35.0 2.0 36.0 84-85 27.17600750397897 32.5 25.5 35.0 2.0 36.0 86-87 26.520001312649384 32.0 24.5 34.5 2.0 35.0 88-89 25.771260271025632 31.5 22.5 34.0 2.0 35.0 90-91 25.219121472938458 31.0 20.0 34.0 2.0 35.0 92-93 24.425530848172045 31.0 17.5 34.0 2.0 35.0 94-95 23.624699503563296 31.0 4.5 34.0 2.0 35.0 96-97 22.628600442289915 30.0 2.0 34.0 2.0 35.0 98-99 21.43358778069275 29.5 2.0 34.0 2.0 35.0 100-101 18.259825271782233 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25489828344188936 1101 2 0.27259955333118313 1101 3 0.25270206980773935 1101 4 0.2237060139717144 1101 5 0.2258602704036221 1101 6 0.19629513399389964 1101 7 0.23931919736678253 1101 8 0.2511780083362609 1101 9 0.4294452129631807 1101 10-11 0.3003487653259853 1101 12-13 0.3185359723374077 1101 14-15 0.5025674993077942 1101 16-17 0.511365009681434 1101 18-19 0.5375764874226974 1101 20-21 0.5423364961257064 1101 22-23 0.4205809321243805 1101 24-25 0.5534447668318947 1101 26-27 0.40269140842887197 1101 28-29 0.4482397535971643 1101 30-31 0.4075591807086383 1101 32-33 0.4365202487295221 1101 34-35 0.3719502633907723 1101 36-37 0.5281644190985446 1101 38-39 0.5874104944837626 1101 40-41 0.7280311861989688 1101 42-43 0.587875304328783 1101 44-45 0.5783268263465544 1101 46-47 0.7210671790462797 1101 48-49 0.8406828870446432 1101 50-51 0.9525840605833409 1101 52-53 0.8849470681644931 1101 54-55 0.9580404889901644 1101 56-57 0.9476083414184089 1101 58-59 1.0200463975191525 1101 60-61 1.1353208589114132 1101 62-63 1.297995430924317 1101 64-65 1.4034568086777313 1101 66-67 1.3618625844571781 1101 68-69 1.4376307329147728 1101 70-71 1.5811997179723427 1101 72-73 1.7410616176366709 1101 74-75 1.754348761629899 1101 76-77 1.701859042755892 1101 78-79 1.988574251206387 1101 80-81 2.2564316814275216 1101 82-83 2.3783581132309095 1101 84-85 2.5479414939800122 1101 86-87 2.5693765301372338 1101 88-89 2.6797271774650966 1101 90-91 2.927249260382826 1101 92-93 3.1788884926626686 1101 94-95 3.297313460497932 1101 96-97 3.7362291346263454 1101 98-99 3.9824963292932516 1101 100-101 4.452956629196986 1102 1 0.2923056649601321 1102 2 0.27990322756831176 1102 3 0.26221365426690113 1102 4 0.19126158506077218 1102 5 0.09500227058155275 1102 6 0.1184082333927492 1102 7 0.21042254878958033 1102 8 0.3003554037075631 1102 9 0.36150900056659907 1102 10-11 0.36938226049012 1102 12-13 0.30901120205665933 1102 14-15 0.47689724652129684 1102 16-17 0.5159171365703443 1102 18-19 0.5471368435351991 1102 20-21 0.508190143038 1102 22-23 0.4887196978043846 1102 24-25 0.47464868000245986 1102 26-27 0.5004302783245222 1102 28-29 0.416338802947088 1102 30-31 0.4060096083740774 1102 32-33 0.35961307055887914 1102 34-35 0.3927257167923912 1102 36-37 0.5002751355175974 1102 38-39 0.6245436744591117 1102 40-41 0.7202469443678297 1102 42-43 0.6842949566043757 1102 44-45 0.5940015909081495 1102 46-47 0.6587109521611865 1102 48-49 0.8968752983660266 1102 50-51 1.0368738690553059 1102 52-53 1.0621934237940494 1102 54-55 0.9589789064373875 1102 56-57 1.1534887412506478 1102 58-59 1.2583591524979951 1102 60-61 1.2630177565894414 1102 62-63 1.423738761758166 1102 64-65 1.4132445010636303 1102 66-67 1.434758095558692 1102 68-69 1.5010642255370676 1102 70-71 1.6997655136334124 1102 72-73 1.8018496849447274 1102 74-75 1.9774085394726377 1102 76-77 1.8564280093687557 1102 78-79 2.2106431860461875 1102 80-81 2.3091331861998334 1102 82-83 2.3589265015740075 1102 84-85 2.5466222763599404 1102 86-87 2.65889761474255 1102 88-89 2.8082382599098423 1102 90-91 3.008764962631087 1102 92-93 3.2465377942907168 1102 94-95 3.4082948669008424 1102 96-97 3.8346450885172736 1102 98-99 4.04983695560389 1102 100-101 4.833468938082003 1103 1 0.22752587414642989 1103 2 0.20989935486424827 1103 3 0.20686834317724845 1103 4 0.22550879618643194 1103 5 0.05386029001740411 1103 6 0.15927580951700548 1103 7 0.21985175440303806 1103 8 0.2029193749018745 1103 9 0.3195781637460797 1103 10-11 0.32941753675557095 1103 12-13 0.3870808474405294 1103 14-15 0.48008619982130796 1103 16-17 0.5207261459567505 1103 18-19 0.4411205902332327 1103 20-21 0.48051899438191725 1103 22-23 0.39484972120636996 1103 24-25 0.4012489581741292 1103 26-27 0.37924688845775023 1103 28-29 0.3181563368031135 1103 30-31 0.40502884983642673 1103 32-33 0.4322753210369399 1103 34-35 0.5282403720388587 1103 36-37 0.6261249077513682 1103 38-39 0.5874135591369125 1103 40-41 0.5650025948238806 1103 42-43 0.6917334115009339 1103 44-45 0.7792710000118603 1103 46-47 0.7107121757548427 1103 48-49 0.8047100650017001 1103 50-51 0.8328981691431636 1103 52-53 1.1151768374696687 1103 54-55 1.0609580541109835 1103 56-57 0.9567168736579461 1103 58-59 1.2262962519481277 1103 60-61 1.2244307058871513 1103 62-63 1.333529394636372 1103 64-65 1.2968539215292907 1103 66-67 1.25081618009213 1103 68-69 1.460434587145162 1103 70-71 1.4165903785031695 1103 72-73 1.3406537276258454 1103 74-75 1.4544837136313014 1103 76-77 1.3173259794389338 1103 78-79 1.736533192209361 1103 80-81 2.05811912542098 1103 82-83 2.1798316444345964 1103 84-85 2.2599753449128315 1103 86-87 2.4272812053269064 1103 88-89 2.619122818670217 1103 90-91 2.863597027415249 1103 92-93 3.089905675559937 1103 94-95 3.004947388905183 1103 96-97 3.2794363768920824 1103 98-99 3.7576101658344605 1103 100-101 4.000716183866107 1104 1 0.2746934475047951 1104 2 0.31368808905375545 1104 3 0.28381103940081687 1104 4 0.15984069756637354 1104 5 0.16428399737112187 1104 6 0.2039720420627944 1104 7 0.21906231780585728 1104 8 0.2528040756093972 1104 9 0.25585024874020945 1104 10-11 0.38773463872596636 1104 12-13 0.37953894862653925 1104 14-15 0.4519448345227204 1104 16-17 0.5250217253925413 1104 18-19 0.5170701039099654 1104 20-21 0.5679608183510325 1104 22-23 0.5305304867465068 1104 24-25 0.5020584956462528 1104 26-27 0.49114159524605583 1104 28-29 0.4520290763670758 1104 30-31 0.546972399504952 1104 32-33 0.6665667399285127 1104 34-35 0.6204171311601883 1104 36-37 0.667877456997104 1104 38-39 0.6778262278537142 1104 40-41 0.6910084106021728 1104 42-43 0.7303266702963214 1104 44-45 0.7325963332343974 1104 46-47 0.721412873726841 1104 48-49 0.9379287540989978 1104 50-51 0.9969046047920074 1104 52-53 1.0487364974841356 1104 54-55 1.0246941526434483 1104 56-57 1.1581775419165936 1104 58-59 1.4580270551914438 1104 60-61 1.2650694252255974 1104 62-63 1.3236878265884542 1104 64-65 1.471571091550686 1104 66-67 1.650715647643537 1104 68-69 1.7826757560952622 1104 70-71 1.7130067658818469 1104 72-73 1.9182215582529523 1104 74-75 1.8512069060545073 1104 76-77 1.6577427188636555 1104 78-79 2.023720863635294 1104 80-81 2.3305544811755894 1104 82-83 2.6935982171287343 1104 84-85 2.723628976517695 1104 86-87 2.9147666560553382 1104 88-89 2.8619571094153358 1104 90-91 3.0045120214888605 1104 92-93 3.38785145597641 1104 94-95 3.6216745172818356 1104 96-97 3.967146965842101 1104 98-99 4.045962972124347 1104 100-101 4.53868950191978 1105 1 0.23251552721821156 1105 2 0.251055236490771 1105 3 0.18498336343677124 1105 4 0.14222134032713996 1105 5 0.130682600966189 1105 6 0.139434085760044 1105 7 0.12002269519170028 1105 8 0.1457824267154635 1105 9 0.15014511203395386 1105 10-11 0.20125635178106904 1105 12-13 0.20896350608891368 1105 14-15 0.38449063191192323 1105 16-17 0.4204435703673326 1105 18-19 0.3828568004791393 1105 20-21 0.3520944974925371 1105 22-23 0.28719573238180374 1105 24-25 0.2822063294414221 1105 26-27 0.46493410336822194 1105 28-29 0.4057862502041374 1105 30-31 0.44930868514730093 1105 32-33 0.42287802526212204 1105 34-35 0.5415185476993827 1105 36-37 0.5266517261637134 1105 38-39 0.38091058181509396 1105 40-41 0.36613594100893465 1105 42-43 0.5226845908728421 1105 44-45 0.5020640827126499 1105 46-47 0.6672234575828142 1105 48-49 0.5670950024204728 1105 50-51 0.588306765439782 1105 52-53 0.758673144361957 1105 54-55 0.791442568882502 1105 56-57 0.7458891597071116 1105 58-59 0.8646158980191103 1105 60-61 1.0388732219565426 1105 62-63 1.1699318742298743 1105 64-65 1.2276545912825014 1105 66-67 1.2732260463180083 1105 68-69 1.3564076869386419 1105 70-71 1.4484741921380966 1105 72-73 1.6186454381303435 1105 74-75 1.7367977284826779 1105 76-77 1.5440952874852414 1105 78-79 1.7844683306110802 1105 80-81 2.2191669192090444 1105 82-83 2.219570339499942 1105 84-85 2.337113991527115 1105 86-87 2.5209027330530454 1105 88-89 2.775003680765515 1105 90-91 2.8316800652494685 1105 92-93 3.0554699809995363 1105 94-95 2.9873569854952073 1105 96-97 3.532160359152268 1105 98-99 3.825514966033598 1105 100-101 4.219783149318946 1106 1 0.058946152519819606 1106 2 0.12370334865858723 1106 3 0.09654674389654616 1106 4 0.13607498038622623 1106 5 0.055290088443506136 1106 6 0.023236707883832253 1106 7 0.06853759596909015 1106 8 -0.021898074781780963 1106 9 0.033302107560139405 1106 10-11 0.14278552535795086 1106 12-13 0.06252039235115348 1106 14-15 0.07567506301241878 1106 16-17 0.037597863797373066 1106 18-19 0.29675929321420114 1106 20-21 0.4042337183995599 1106 22-23 0.26557389255409447 1106 24-25 0.19866610672000462 1106 26-27 0.2432004181495202 1106 28-29 0.33188762174578557 1106 30-31 0.34109622789931393 1106 32-33 0.22559741450093895 1106 34-35 0.22987872462899617 1106 36-37 0.38652210826086275 1106 38-39 0.41531739558593017 1106 40-41 0.33829339276737613 1106 42-43 0.5303529542739511 1106 44-45 0.008016504431417104 1106 46-47 0.010117626976693828 1106 48-49 0.43098105815035126 1106 50-51 0.28682831740916725 1106 52-53 0.5062338690863797 1106 54-55 0.5759335432808186 1106 56-57 0.6038641496447141 1106 58-59 0.8863909786763386 1106 60-61 0.8985630340689355 1106 62-63 1.102440582681652 1106 64-65 0.7364598475417381 1106 66-67 0.7586608577876603 1106 68-69 0.631601677843495 1106 70-71 0.8847196192789646 1106 72-73 1.2971040316284537 1106 74-75 1.651485654554083 1106 76-77 1.5662845859412187 1106 78-79 1.806335342595343 1106 80-81 1.8633315057867534 1106 82-83 2.123331674184758 1106 84-85 2.2913479954762117 1106 86-87 2.470661077467309 1106 88-89 2.8066131085470047 1106 90-91 2.626032320806967 1106 92-93 2.38180933350797 1106 94-95 2.916732916662088 1106 96-97 3.3354400290002637 1106 98-99 3.682392245877832 1106 100-101 4.003706006555124 1107 1 0.17596810139207264 1107 2 0.11679401198738759 1107 3 0.1595470931131402 1107 4 0.08225133781347438 1107 5 0.05414739387200029 1107 6 0.009529321952790326 1107 7 0.09964870877214338 1107 8 0.09147232728428634 1107 9 0.2091429429593319 1107 10-11 0.20383993107048326 1107 12-13 0.207286343631381 1107 14-15 0.2843802638018076 1107 16-17 0.2976819371856507 1107 18-19 0.3490730054475435 1107 20-21 0.2938074838072211 1107 22-23 0.3156492304516121 1107 24-25 0.32480708820137494 1107 26-27 0.34204096067374223 1107 28-29 0.32788184596720527 1107 30-31 0.27541565398620094 1107 32-33 0.40905204730577793 1107 34-35 0.48011525796395205 1107 36-37 0.5179071759012785 1107 38-39 0.6031200415107563 1107 40-41 0.6480665339503702 1107 42-43 0.7383643535564346 1107 44-45 0.7483589270403499 1107 46-47 0.6201230758722929 1107 48-49 0.7834997321018307 1107 50-51 0.7802673725143237 1107 52-53 0.8669878749255986 1107 54-55 0.789131164344262 1107 56-57 0.8555481172528445 1107 58-59 0.8560586239616086 1107 60-61 0.9791770588714357 1107 62-63 1.128718624063822 1107 64-65 1.2751753040307676 1107 66-67 1.3023246068714798 1107 68-69 1.4038210279960772 1107 70-71 1.3880996730217738 1107 72-73 1.5459897616637193 1107 74-75 1.61622930774546 1107 76-77 1.6679123261240676 1107 78-79 1.754414245243094 1107 80-81 1.9936797097576715 1107 82-83 2.0336916312229967 1107 84-85 2.164683379441893 1107 86-87 2.3856352110751438 1107 88-89 2.6707391102606053 1107 90-91 2.85844890981355 1107 92-93 3.047257295159117 1107 94-95 3.3131077133753912 1107 96-97 3.6584719168408277 1107 98-99 3.9606393257570645 1107 100-101 4.2842361280094075 1108 1 0.19503229332129024 1108 2 0.23716132635923515 1108 3 0.1715812294897887 1108 4 0.11000715504373204 1108 5 0.0903620119576729 1108 6 0.10351583567901912 1108 7 0.12707776767526724 1108 8 0.20641998564419595 1108 9 0.17263555818458798 1108 10-11 0.13918746998451326 1108 12-13 0.270536375521381 1108 14-15 0.3531731257040178 1108 16-17 0.3489157422717497 1108 18-19 0.387060671234444 1108 20-21 0.26451199157357763 1108 22-23 0.30719024684545104 1108 24-25 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0.7767728294330922 2316 74-75 1.09357015563425 2316 76-77 0.9253051869415856 2316 78-79 1.0563246382493787 2316 80-81 1.172147386170554 2316 82-83 1.3843620161399564 2316 84-85 1.6149870838551834 2316 86-87 1.694543932997565 2316 88-89 1.9299847099171643 2316 90-91 2.160375268589224 2316 92-93 2.1711974341677482 2316 94-95 2.659678266110326 2316 96-97 2.8516557750931817 2316 98-99 3.2199438213629286 2316 100-101 3.61272526951225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 42.0 5 98.0 6 204.0 7 528.0 8 1129.0 9 1931.0 10 3095.0 11 4787.0 12 6918.0 13 10010.0 14 12774.0 15 16905.0 16 21093.0 17 25949.0 18 25796.0 19 23802.0 20 25277.0 21 27374.0 22 29844.0 23 34160.0 24 39805.0 25 45968.0 26 54875.0 27 64592.0 28 77510.0 29 91812.0 30 107811.0 31 127207.0 32 152552.0 33 184179.0 34 229083.0 35 288253.0 36 363886.0 37 409956.0 38 222093.0 39 11221.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54453436497852 18.998886071149244 14.599395816659344 23.857183747212897 2 16.08993807072639 20.31048172198181 38.62625499160451 24.97332521568729 3 19.53410376213017 23.673548117038866 29.444580976335093 27.347767144495872 4 13.741579445369172 16.664193294914032 35.590737800109025 34.00348945960777 5 14.565331807195673 36.87370748525265 33.58950644094818 14.971454266603498 6 33.44944203285634 35.174044546215285 16.853068956024423 14.523444464903948 7 29.9114143979406 31.65238856609463 19.881424005804828 18.554773030159943 8 27.067778286226847 34.87888074762675 19.214671044595438 18.83866992155097 9 26.616482136118098 16.483959242236683 19.595302902960572 37.30425571868465 10-11 25.652906333350955 26.207427772379305 26.80014366218239 21.339522232087347 12-13 26.206953760102387 23.21376677502101 27.35178456506639 23.227494899810214 14-15 23.00405280496765 24.8055364633944 25.27816316596446 26.912247565673493 16-17 23.11657602701141 28.079247560204116 25.899556798521083 22.904619614263392 18-19 23.14197214630936 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27.416391816671865 23.404329411593107 44-45 23.0655142545277 26.277443459467975 27.335901690422848 23.32114059558148 46-47 23.239298986634306 26.40121726397097 26.940989825505078 23.418493923889645 48-49 23.187295012479282 26.36459930466045 27.045864334040097 23.402241348820166 50-51 23.306800799986874 26.40242913060679 26.954088264732214 23.336681804674125 52-53 23.671535015833832 26.318765963730765 26.75281535945627 23.256883660979128 54-55 23.07141724201426 26.33491884362882 27.02938329179649 23.56428062256043 56-57 23.143175408243074 26.510029917467172 27.00353139146304 23.34326328282672 58-59 23.21615506764702 26.114247897482084 26.988709392188643 23.68088764268225 60-61 23.18135162777639 26.29776357361502 27.11107748459916 23.40980731400943 62-63 23.016504742857453 26.421845402719008 27.028161798621355 23.533488055802184 64-65 22.837638033286602 26.155796896678083 27.05459709868024 23.951967971355074 66-67 22.7595321273228 26.403288045648175 26.949478603966178 23.887701223062844 68-69 23.00203606726898 26.13653683334658 27.04150018486499 23.81992691451945 70-71 22.845732704477044 26.28846564049086 26.885429409544788 23.98037224548731 72-73 22.95462790947824 26.21508489377567 26.7875276431198 24.042759553626286 74-75 22.78848660641849 26.3962122772826 26.68645364068776 24.128847475611156 76-77 22.81672679937795 26.186224838607934 26.93224723751114 24.06480112450297 78-79 22.892258832580687 26.268447737411783 26.8345642459832 24.004729184024328 80-81 22.648839426306484 26.437747865166816 26.87593583258175 24.03747687594495 82-83 22.824414217859307 26.323145397726204 26.72644405660001 24.12599632781448 84-85 22.689697357541814 26.103917058883052 26.851047110634646 24.35533847294049 86-87 22.7838203158358 26.127830035431167 26.80334483013862 24.285004818594413 88-89 22.539784229532874 26.186580780472436 26.72524123587443 24.548393754120262 90-91 22.43592104327597 26.14720429970301 26.97564097188165 24.44123368513937 92-93 22.5281067157307 25.877813565789502 26.943450383650035 24.65062933482976 94-95 22.300673279745638 25.76346058132136 27.006339002641706 24.92952713629129 96-97 22.40189721625301 25.721187021760265 26.92062061344857 24.956295148538153 98-99 22.21343678955131 25.789403637861913 26.740618658946342 25.256540913640436 100-101 22.173761247608272 25.504393301434536 27.02745915947971 25.294386291477476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 109.0 2 101.5 3 136.5 4 135.0 5 144.5 6 265.5 7 568.5 8 905.0 9 1065.0 10 1069.5 11 1077.5 12 1170.5 13 1322.5 14 1502.5 15 1702.5 16 1838.5 17 1921.0 18 2125.0 19 2398.5 20 2672.0 21 2995.5 22 3542.5 23 4268.5 24 5242.5 25 6680.5 26 8822.5 27 11863.5 28 14886.0 29 18395.0 30 22717.5 31 26859.0 32 32095.5 33 36912.0 34 40621.5 35 45167.5 36 51082.0 37 57183.0 38 62742.0 39 66935.5 40 71548.5 41 79853.0 42 88394.5 43 95797.5 44 100998.0 45 104745.5 46 107233.0 47 110985.0 48 118222.0 49 119979.5 50 117862.0 51 114669.5 52 109477.0 53 107483.0 54 106012.5 55 98075.0 56 87125.5 57 79109.0 58 72378.0 59 63775.5 60 52809.5 61 42882.5 62 35095.5 63 26815.0 64 20216.5 65 16041.5 66 12233.0 67 9580.5 68 7405.0 69 5386.5 70 4157.5 71 3385.5 72 2862.0 73 2410.5 74 1920.0 75 1577.5 76 1127.5 77 660.0 78 388.0 79 245.5 80 140.5 81 71.5 82 40.5 83 24.0 84 9.5 85 6.5 86 5.0 87 4.5 88 2.5 89 2.0 90 1.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04346327954509406 3 1.0938744851953204E-4 4 0.0 5 0.099579040635614 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.115620709961003E-4 26-27 0.011321600921771566 28-29 0.0 30-31 0.0 32-33 4.0108731123828417E-4 34-35 0.014876692998656358 36-37 0.003062848558546897 38-39 0.011285138438931721 40-41 0.007146646636609427 42-43 0.005104747597578162 44-45 0.002388292626009783 46-47 3.646248283984401E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4584993135937605E-4 68-69 1.0938744851953204E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8231241419922006E-5 82-83 0.0013308806236543066 84-85 0.0042843417336816715 86-87 0.0016043492449531367 88-89 3.646248283984401E-5 90-91 0.0010938744851953204 92-93 2.3700613845898606E-4 94-95 0.0 96-97 1.8231241419922006E-5 98-99 0.0 100-101 6.380934496972703E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2742545.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.744047282505846 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7174793269476 33.52365244640974 2 14.549196622367225 13.60176669676816 3 5.476176068259211 7.679358991861067 4 2.567002108819314 4.7996827179576895 5 1.4494035573520008 3.3875494208147052 6 0.8973634021757838 2.5167838380537093 7 0.6162210263688872 2.0163265355143083 8 0.418814504469647 1.566166799962275 9 0.31294289870734887 1.3165395888510678 >10 1.769181015261571 15.092575261826225 >50 0.13653646963063007 4.400924375253381 >100 0.08281647561904572 7.111289683173105 >500 0.005071864334234272 1.5308403504552541 >1k 0.0017166310054191334 1.2696881999320004 >5k 7.802868206450606E-5 0.18685509316743668 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5123 0.18679729958852087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4459 0.16258621098286444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.10610582506394607 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.646248283984401E-5 0.0 14-15 0.0 0.0 0.0 3.646248283984401E-5 0.0 16-17 0.0 0.0 0.0 7.292496567968802E-5 0.0 18-19 0.0 0.0 0.0 1.2761868993945404E-4 0.0 20-21 0.0 0.0 0.0 1.6408117277929806E-4 0.0 22-23 0.0 1.8231241419922006E-5 0.0 3.281623455585961E-4 0.0 24-25 0.0 3.646248283984401E-5 0.0 5.833997254375042E-4 0.0 26-27 0.0 3.646248283984401E-5 0.0 0.0012214931751347744 0.0 28-29 0.0 3.646248283984401E-5 0.0 0.004010873112382842 0.0 30-31 0.0 3.646248283984401E-5 0.0 0.012488400372646575 0.0 32-33 0.0 3.646248283984401E-5 0.0 0.03061025434404905 0.0 34-35 0.0 3.646248283984401E-5 0.0 0.059470309511785585 0.0 36-37 0.0 3.646248283984401E-5 0.0 0.10944214224379181 0.0 38-39 0.0 3.646248283984401E-5 0.0 0.1834062886844154 0.0 40-41 0.0 3.646248283984401E-5 0.0 0.2663402059036406 0.0 42-43 0.0 3.646248283984401E-5 0.0 0.347833855050692 0.0 44-45 0.0 3.646248283984401E-5 0.0 0.4519160123170267 0.0 46-47 0.0 3.646248283984401E-5 0.0 0.5767635535606526 0.0 48-49 0.0 3.646248283984401E-5 0.0 0.7097239972361438 0.0 50-51 0.0 3.646248283984401E-5 0.0 0.8526751612097523 0.0 52-53 0.0 3.646248283984401E-5 0.0 0.9942225195940266 3.646248283984401E-5 54-55 0.0 3.646248283984401E-5 0.0 1.1492609966290435 7.292496567968802E-5 56-57 0.0 3.646248283984401E-5 0.0 1.3354931277335467 7.292496567968802E-5 58-59 0.0 3.646248283984401E-5 0.0 1.5335026407953198 7.292496567968802E-5 60-61 0.0 3.646248283984401E-5 0.0 1.749615776587075 7.292496567968802E-5 62-63 0.0 3.646248283984401E-5 0.0 1.966129999690069 7.292496567968802E-5 64-65 0.0 3.646248283984401E-5 0.0 2.19961750855501 7.292496567968802E-5 66-67 0.0 3.646248283984401E-5 0.0 2.458865761546301 7.292496567968802E-5 68-69 0.0 3.646248283984401E-5 0.0 2.751440723853209 7.292496567968802E-5 70-71 0.0 3.646248283984401E-5 0.0 3.062374546269979 1.0938744851953204E-4 72-73 0.0 3.646248283984401E-5 0.0 3.3811660337387353 1.0938744851953204E-4 74-75 0.0 3.646248283984401E-5 0.0 3.7156363888286243 1.0938744851953204E-4 76-77 0.0 3.646248283984401E-5 0.0 4.077289524875617 1.0938744851953204E-4 78-79 0.0 3.646248283984401E-5 0.0 4.471558351822851 1.0938744851953204E-4 80-81 0.0 3.646248283984401E-5 0.0 4.893411047038426 1.0938744851953204E-4 82-83 0.0 3.646248283984401E-5 0.0 5.330377441391117 1.0938744851953204E-4 84-85 0.0 7.292496567968802E-5 0.0 5.766468736155652 1.0938744851953204E-4 86-87 0.0 7.292496567968802E-5 0.0 6.225713707523487 1.0938744851953204E-4 88-89 0.0 7.292496567968802E-5 0.0 6.719598037589174 1.0938744851953204E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4835 0.0 42.49466 1 GTATCAA 7975 0.0 36.25935 1 TCAACGC 9970 0.0 28.468706 4 ATCAACG 9985 0.0 28.378326 3 TATCAAC 10225 0.0 27.81538 2 CAACGCA 10210 0.0 27.79951 5 AACGCAG 10565 0.0 26.837976 6 GTACATG 10990 0.0 24.191412 1 TACATGG 11100 0.0 23.351814 2 ACGCAGA 12340 0.0 22.823616 7 CGCAGAG 12430 0.0 22.658361 8 ACATGGG 11510 0.0 22.05741 3 TAAGGTG 1155 0.0 20.581478 5 GGACCGA 790 0.0 20.440748 6 GCAGAGT 13750 0.0 20.310452 9 CATGGGG 6110 0.0 20.308968 4 GAGTACT 5930 0.0 19.902912 12-13 GTGTAAG 1200 0.0 19.816906 1 AGTACTT 6335 0.0 19.155312 12-13 AGAGTAC 12490 0.0 18.784924 10-11 >>END_MODULE