FastQCFastQC Report
Thu 26 May 2016
SRR938364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737422
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.17823338893309107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.148059013188321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.044.4612051
GTATCAA80000.037.851261
TATCAAC99550.030.0812452
ATCAACG100150.029.8061073
TCAACGC100850.029.4106924
CAACGCA102450.028.8580535
AACGCAG106150.027.8887866
GTACATG116050.025.8465211
TACATGG117200.024.9832922
ACGCAGA122750.023.9567287
ACATGGG119750.023.6177233
CGCAGAG125300.023.545018
GAGTACT58150.020.82981112-13
CATGGGG67800.020.5416094
GCAGAGT138100.020.5359699
GGACCGA6900.019.971496
GTACTTT62650.019.56343314-15
AGTACTT60200.019.37090512-13
AGAGTAC123150.018.822810-11
GTGGTAT29150.018.8129061
Produced by FastQC (version 0.11.5)