##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2737422 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04365603841863 31.0 31.0 34.0 28.0 34.0 2 31.21178101147722 31.0 31.0 34.0 28.0 34.0 3 31.268963645356834 31.0 31.0 34.0 27.0 34.0 4 34.9342344731649 37.0 35.0 37.0 32.0 37.0 5 34.92138296543244 37.0 35.0 37.0 32.0 37.0 6 34.89306727278439 37.0 35.0 37.0 32.0 37.0 7 34.806631202642485 37.0 35.0 37.0 32.0 37.0 8 34.76531678345538 37.0 35.0 37.0 32.0 37.0 9 36.23889593931809 39.0 35.0 39.0 32.0 39.0 10-11 36.07030099853073 38.5 35.0 39.0 30.0 39.0 12-13 35.88114090556736 38.0 35.0 39.0 30.0 39.0 14-15 37.03280915401425 39.0 36.0 41.0 31.0 41.0 16-17 36.95896778063448 39.0 36.0 41.0 31.0 41.0 18-19 36.93668659782817 39.0 36.0 41.0 31.0 41.0 20-21 36.85726278228202 39.0 36.0 41.0 30.0 41.0 22-23 36.70160519642204 39.0 36.0 41.0 30.0 41.0 24-25 36.55962891362749 39.0 36.0 41.0 30.0 41.0 26-27 36.34116825977142 39.0 35.5 40.5 29.0 41.0 28-29 36.114378236165265 39.0 35.0 40.0 28.5 41.0 30-31 35.86067694348917 38.5 35.0 40.0 27.0 41.0 32-33 35.59414149517319 38.0 34.5 40.0 27.0 41.0 34-35 35.28869754097103 38.0 34.0 40.0 26.0 41.0 36-37 35.104154017904435 38.0 34.0 40.0 25.0 41.0 38-39 34.88513736647108 38.0 34.0 40.0 25.0 41.0 40-41 34.62316387462364 38.0 33.0 40.0 24.0 41.0 42-43 34.23100566883732 38.0 33.0 40.0 24.0 41.0 44-45 33.788313968398 38.0 33.0 40.0 22.5 41.0 46-47 33.326654421568904 37.5 32.0 40.0 19.5 41.0 48-49 32.87820237435076 37.0 31.0 40.0 16.5 41.0 50-51 32.16121226467823 36.5 30.5 39.0 15.0 40.5 52-53 32.170284303991124 36.5 31.0 39.0 13.5 40.0 54-55 32.16026831084137 36.5 31.0 39.0 10.5 40.0 56-57 31.738616661954204 36.0 30.0 39.0 8.0 40.0 58-59 31.287667739939256 36.0 29.5 39.0 4.5 40.0 60-61 30.73418767731099 35.0 28.5 39.0 2.0 40.0 62-63 30.00452633901532 34.5 28.0 38.0 2.0 40.0 64-65 29.34151420570157 34.0 26.5 38.0 2.0 40.0 66-67 28.649054292688525 34.0 26.0 37.5 2.0 39.5 68-69 27.810461266110963 33.5 24.5 36.5 2.0 39.0 70-71 26.984996650132864 32.5 22.5 36.0 2.0 39.0 72-73 26.085924274737323 32.0 20.5 35.5 2.0 38.5 74-75 25.145272815079295 31.0 16.5 35.0 2.0 37.5 76-77 24.198418986915428 30.5 8.5 34.5 2.0 37.0 78-79 23.215995012825935 30.0 4.0 34.0 2.0 36.0 80-81 22.247079734144023 29.0 2.0 34.0 2.0 35.5 82-83 21.241706613010344 28.5 2.0 34.0 2.0 35.0 84-85 20.254299483236416 26.5 2.0 33.0 2.0 35.0 86-87 19.25439154065394 25.5 2.0 32.5 2.0 35.0 88-89 18.15947066254308 24.0 2.0 32.0 2.0 34.0 90-91 16.99980510860218 19.0 2.0 31.5 2.0 34.0 92-93 15.834271442254792 11.0 2.0 31.0 2.0 34.0 94-95 14.562088892395838 2.0 2.0 30.5 2.0 34.0 96-97 13.126168526445685 2.0 2.0 29.5 2.0 34.0 98-99 11.41745792208874 2.0 2.0 28.0 2.0 34.0 100-101 8.102214236606558 2.0 2.0 10.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7206045570305655 1101 2 0.6795661765462349 1101 3 0.5981527107925722 1101 4 0.5622212169289469 1101 5 0.5218598998350146 1101 6 0.5665014252948524 1101 7 0.4891717734633687 1101 8 0.5175730649439103 1101 9 0.5007010682010602 1101 10-11 0.7042439638890983 1101 12-13 0.6749445104092544 1101 14-15 0.8254749918030981 1101 16-17 0.8833645830966717 1101 18-19 0.9156940160029237 1101 20-21 1.0529746357101288 1101 22-23 1.001233428422367 1101 24-25 1.0411802459343846 1101 26-27 1.0401730580671682 1101 28-29 0.9895892030675384 1101 30-31 1.1737934316186909 1101 32-33 1.1038361269171801 1101 34-35 1.1082988876493047 1101 36-37 1.3366148490740102 1101 38-39 1.3809384695028015 1101 40-41 1.3103201291549311 1101 42-43 1.4840632910949552 1101 44-45 1.3905818699883312 1101 46-47 1.3302461802764256 1101 48-49 1.2490126972864175 1101 50-51 1.5386039147684443 1101 52-53 1.4599061056114664 1101 54-55 1.656388028505802 1101 56-57 1.8336674477926493 1101 58-59 1.8071363541726377 1101 60-61 2.061395745332046 1101 62-63 1.7726307630833311 1101 64-65 1.8738798471818328 1101 66-67 2.231183560657019 1101 68-69 2.187355887407886 1101 70-71 2.582791996720566 1101 72-73 2.644525827327083 1101 74-75 2.538708101927771 1101 76-77 2.864427463232232 1101 78-79 3.1309614625632207 1101 80-81 3.5176691551810926 1101 82-83 3.411152396585816 1101 84-85 3.249932379263676 1101 86-87 3.625018431161262 1101 88-89 3.7604630690409806 1101 90-91 3.872062595600198 1101 92-93 3.889957402033442 1101 94-95 3.883856217811248 1101 96-97 3.7486938639595717 1101 98-99 3.4581624401264666 1101 100-101 2.8992866200748555 1102 1 0.6974594182098564 1102 2 0.4999334531659265 1102 3 0.4467727939721833 1102 4 0.2726543749678143 1102 5 0.3055283156273987 1102 6 0.5500352872431833 1102 7 0.5900289177298816 1102 8 0.6471218751623624 1102 9 0.6012092571932044 1102 10-11 0.730861390680495 1102 12-13 0.7327011433043253 1102 14-15 0.7912997316022725 1102 16-17 0.827121999191867 1102 18-19 0.7560895432014689 1102 20-21 0.7999951893826491 1102 22-23 0.4846622696314853 1102 24-25 0.8018169771288655 1102 26-27 0.7691262256597398 1102 28-29 0.8196577704716788 1102 30-31 0.7573607410812286 1102 32-33 0.7640076321492089 1102 34-35 0.6455629510003575 1102 36-37 0.8026087359122585 1102 38-39 0.8377900623093879 1102 40-41 1.0849536584898516 1102 42-43 1.2593990272815816 1102 44-45 1.2907992788307254 1102 46-47 1.2837622507568014 1102 48-49 1.3454857826191073 1102 50-51 1.2309018210527043 1102 52-53 1.264438959838067 1102 54-55 1.1217225123996784 1102 56-57 1.4813265275595597 1102 58-59 1.5720657285211352 1102 60-61 1.586114763139907 1102 62-63 1.684386370912339 1102 64-65 2.0274519923720504 1102 66-67 2.0746527743842478 1102 68-69 1.972011418587666 1102 70-71 1.9020966544062645 1102 72-73 2.01905373125793 1102 74-75 2.017313786127545 1102 76-77 2.188132728574292 1102 78-79 2.538300868747317 1102 80-81 2.9944792234238875 1102 82-83 3.3787021815876805 1102 84-85 3.1039204543178585 1102 86-87 3.212110300280596 1102 88-89 3.3671759946912303 1102 90-91 3.493805978060429 1102 92-93 3.5060988032296905 1102 94-95 3.2170847956092583 1102 96-97 2.9265700359154376 1102 98-99 3.019388417229081 1102 100-101 2.3119492251970364 1103 1 0.4177867754259985 1103 2 0.3878405705039647 1103 3 0.34125110003647663 1103 4 0.34939270224088403 1103 5 0.4225838373887072 1103 6 0.35100451924008524 1103 7 0.27171275658206895 1103 8 0.3463476483673489 1103 9 0.3203316993500209 1103 10-11 0.35887575563950946 1103 12-13 0.3242782061412157 1103 14-15 0.3991801276132989 1103 16-17 0.45275168633464347 1103 18-19 0.6076733672167904 1103 20-21 0.4820876782913075 1103 22-23 0.528304209239316 1103 24-25 0.49895121661381836 1103 26-27 0.6003517311723598 1103 28-29 0.5656829955361218 1103 30-31 0.59479714355939 1103 32-33 0.798381696891532 1103 34-35 0.6461067198243455 1103 36-37 0.7480014404569957 1103 38-39 0.7275932590173184 1103 40-41 0.8290077964253726 1103 42-43 0.8678612948604609 1103 44-45 0.9678699907861059 1103 46-47 1.1841244738550287 1103 48-49 1.0302512276673923 1103 50-51 1.0067905394815213 1103 52-53 1.1062252134759802 1103 54-55 1.1441580667362103 1103 56-57 1.3878476961415203 1103 58-59 1.500814944944569 1103 60-61 1.51017651862988 1103 62-63 1.6512774819539153 1103 64-65 1.7235373209591813 1103 66-67 1.8651516867717426 1103 68-69 1.8762551809289505 1103 70-71 1.8402362134534656 1103 72-73 1.903865693094101 1103 74-75 2.0006314466819504 1103 76-77 2.159792596383973 1103 78-79 2.376905805275932 1103 80-81 2.4337814775941915 1103 82-83 2.65838530449755 1103 84-85 2.793298202824481 1103 86-87 2.865837280122374 1103 88-89 2.9534978731969233 1103 90-91 2.832343242918217 1103 92-93 2.833581930601099 1103 94-95 2.7914177842462617 1103 96-97 2.6443607534881703 1103 98-99 2.487105062768313 1103 100-101 1.9681420289735936 1104 1 0.5079873313243333 1104 2 0.417153196855768 1104 3 0.41689156356283874 1104 4 0.33593964509763197 1104 5 0.2979659926950404 1104 6 0.4602071959639744 1104 7 0.3819857657537966 1104 8 0.46403759517807686 1104 9 0.469402523813514 1104 10-11 0.47192131271828686 1104 12-13 0.4652615232393629 1104 14-15 0.5471969870853002 1104 16-17 0.6103509475175954 1104 18-19 0.5219163277226215 1104 20-21 0.5657903540232283 1104 22-23 0.60293931415967 1104 24-25 0.7460447531549121 1104 26-27 0.5881564882495525 1104 28-29 0.768658301508367 1104 30-31 0.7271131485509699 1104 32-33 0.7172645832775757 1104 34-35 0.716448591348744 1104 36-37 0.8234107756605198 1104 38-39 0.8186578182169129 1104 40-41 0.8506515255072671 1104 42-43 0.7221825017272536 1104 44-45 0.7428630966151033 1104 46-47 0.8072756952640603 1104 48-49 0.7562476089715915 1104 50-51 0.7831957104073624 1104 52-53 0.8472399759296323 1104 54-55 1.0548115944686316 1104 56-57 1.1792171860125364 1104 58-59 1.50263718841693 1104 60-61 1.5784402191827986 1104 62-63 1.62095652109495 1104 64-65 1.5604916646837026 1104 66-67 1.774636181334774 1104 68-69 2.0925109417776007 1104 70-71 2.129624745123998 1104 72-73 2.3126116681877917 1104 74-75 2.16141393013098 1104 76-77 2.394258888351615 1104 78-79 2.46333589441409 1104 80-81 2.971857636856484 1104 82-83 3.079684126635872 1104 84-85 3.075963677808062 1104 86-87 2.936205934757144 1104 88-89 2.8396731259997274 1104 90-91 2.719079453088259 1104 92-93 2.904010103258919 1104 94-95 2.849666585454079 1104 96-97 2.66837627578858 1104 98-99 2.5038876573176516 1104 100-101 1.51608082063869 1105 1 0.547027802707305 1105 2 0.3445669545803547 1105 3 0.3527190814135466 1105 4 0.3052496935525042 1105 5 0.35433643775990475 1105 6 0.5411100698214781 1105 7 0.4316606119424762 1105 8 0.38268910409935586 1105 9 0.5103008454376123 1105 10-11 0.6064171288528755 1105 12-13 0.5409976388607518 1105 14-15 0.7113173426931709 1105 16-17 0.5714963945770606 1105 18-19 0.6662376700390951 1105 20-21 0.5991371314769225 1105 22-23 0.6271898967308047 1105 24-25 0.7297795244532139 1105 26-27 0.5503537387112445 1105 28-29 0.6027502069916153 1105 30-31 0.709550026337908 1105 32-33 0.8216244007719737 1105 34-35 0.8780464065969085 1105 36-37 0.9564995620900589 1105 38-39 0.9973408512211464 1105 40-41 1.1335392104884505 1105 42-43 1.2035893900088794 1105 44-45 1.2911652016094521 1105 46-47 1.4457751953455826 1105 48-49 1.3263069747915708 1105 50-51 1.3186158087046493 1105 52-53 1.4105486411032544 1105 54-55 1.5779876925215035 1105 56-57 1.7420397415018911 1105 58-59 1.7620923453155477 1105 60-61 2.0002442031074956 1105 62-63 1.9536949643325237 1105 64-65 2.051228163215697 1105 66-67 2.20364302062519 1105 68-69 2.337286079858899 1105 70-71 2.392484204123342 1105 72-73 2.36531337602246 1105 74-75 2.612500435640758 1105 76-77 2.66760983112556 1105 78-79 2.8029820049629173 1105 80-81 2.9816512760575655 1105 82-83 3.2996492590895876 1105 84-85 3.2374905145048274 1105 86-87 3.479946533768082 1105 88-89 3.4414368229255814 1105 90-91 3.2998958718681166 1105 92-93 3.244348855066402 1105 94-95 3.182230650934729 1105 96-97 3.1209844840577983 1105 98-99 3.080145905572339 1105 100-101 2.38477610411395 1106 1 0.2508733811014636 1106 2 0.20100110099710378 1106 3 0.03838058281067802 1106 4 0.14952744846318922 1106 5 0.20120335380611465 1106 6 -0.22696925577978533 1106 7 0.22590310533309577 1106 8 -0.2172555897147035 1106 9 0.2445283464640795 1106 10-11 0.2623625884841445 1106 12-13 0.4554322345127133 1106 14-15 0.39796902379842436 1106 16-17 -0.02993458920850145 1106 18-19 0.41069534862571544 1106 20-21 0.3448918141034696 1106 22-23 0.20338705052856199 1106 24-25 0.30266481220418484 1106 26-27 0.3195264329145928 1106 28-29 0.4411050596948911 1106 30-31 0.3563013427616184 1106 32-33 0.49884820115644146 1106 34-35 0.4113692764828514 1106 36-37 0.5001322014642611 1106 38-39 0.6533917956673818 1106 40-41 0.7660658932359183 1106 42-43 0.359723752918228 1106 44-45 0.8476890547618368 1106 46-47 0.9905640860471081 1106 48-49 0.6846836617035876 1106 50-51 0.5143493879906416 1106 52-53 0.8648013309341493 1106 54-55 0.7068970241776213 1106 56-57 1.1453458167257082 1106 58-59 1.2440284215176796 1106 60-61 1.4780999812582571 1106 62-63 1.506409374336311 1106 64-65 1.440208392912389 1106 66-67 1.6840844522543819 1106 68-69 1.749903772914923 1106 70-71 1.4762885095109368 1106 72-73 1.8051671335076414 1106 74-75 2.0768234176441815 1106 76-77 2.119087071341781 1106 78-79 2.4071016397941314 1106 80-81 2.2579948968601897 1106 82-83 2.5600746855983694 1106 84-85 2.512113278740145 1106 86-87 2.311377845360152 1106 88-89 2.2733145636015593 1106 90-91 2.4378742190371376 1106 92-93 2.5370534002629483 1106 94-95 2.2183819041695845 1106 96-97 1.941402135047447 1106 98-99 2.1865944333831493 1106 100-101 1.7396140752289675 1107 1 0.27008968676195977 1107 2 0.21350992296468618 1107 3 0.23907332800870762 1107 4 0.15733056011599444 1107 5 0.23473420420351232 1107 6 0.2258047921757793 1107 7 0.16738026364024705 1107 8 0.2800730038700294 1107 9 0.24917928157714897 1107 10-11 0.38055260266745705 1107 12-13 0.3706202702206838 1107 14-15 0.31724977087269224 1107 16-17 0.3877645548979842 1107 18-19 0.43479073678919633 1107 20-21 0.49539250529959133 1107 22-23 0.4758830213911267 1107 24-25 0.4722675494771593 1107 26-27 0.4377116759547661 1107 28-29 0.5086982782274987 1107 30-31 0.5588278179588002 1107 32-33 0.7145771729626986 1107 34-35 0.7586510570982554 1107 36-37 0.8229166405977963 1107 38-39 0.8786864137559576 1107 40-41 0.6741212558458898 1107 42-43 0.8779371271140235 1107 44-45 0.6654591463863753 1107 46-47 0.6469010625922991 1107 48-49 0.7528738927570373 1107 50-51 0.9042149223060036 1107 52-53 0.9575346940509846 1107 54-55 1.0574290066371645 1107 56-57 1.261054092924084 1107 58-59 1.1666003279115635 1107 60-61 1.3651122480531477 1107 62-63 1.3704332201379223 1107 64-65 1.49235054323562 1107 66-67 1.7031713793841554 1107 68-69 1.7146102639622178 1107 70-71 1.7554620979496534 1107 72-73 1.8542666576945486 1107 74-75 2.0867157323239987 1107 76-77 2.2791574364085854 1107 78-79 2.5031405995857874 1107 80-81 2.5583979655107463 1107 82-83 2.778123362411666 1107 84-85 2.716472058789826 1107 86-87 2.818877958442439 1107 88-89 3.1021250184773876 1107 90-91 3.098747065594079 1107 92-93 3.0694812491156114 1107 94-95 2.844250608506803 1107 96-97 2.5888025126493464 1107 98-99 2.2414170986676307 1107 100-101 1.6864220875273634 1108 1 0.2974905863749804 1108 2 0.2813839959667668 1108 3 0.1791098725816731 1108 4 0.1564254171674122 1108 5 0.27823283525275144 1108 6 0.2393707588950349 1108 7 0.2511919355485759 1108 8 0.2045650448950198 1108 9 0.22467582772673467 1108 10-11 0.2236573735814531 1108 12-13 0.1773791925205188 1108 14-15 0.1645443429564537 1108 16-17 0.3216351146323646 1108 18-19 0.31310448594940965 1108 20-21 0.40479190830293277 1108 22-23 0.4472480659070328 1108 24-25 0.3980301791803953 1108 26-27 0.43608740734627105 1108 28-29 0.4223853738516823 1108 30-31 0.49551569836179965 1108 32-33 0.4630941527761152 1108 34-35 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0.39375701612423875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9051.0 3 3535.0 4 3724.0 5 4486.0 6 5365.0 7 6877.0 8 8881.0 9 11242.0 10 14318.0 11 17382.0 12 21541.0 13 25195.0 14 28538.0 15 32657.0 16 37417.0 17 42423.0 18 43435.0 19 44523.0 20 48910.0 21 54335.0 22 60128.0 23 66917.0 24 74237.0 25 82627.0 26 90976.0 27 100771.0 28 111379.0 29 124124.0 30 137517.0 31 153661.0 32 172323.0 33 194921.0 34 219087.0 35 240566.0 36 238512.0 37 166826.0 38 38593.0 39 422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81249170322138 18.185621948527338 14.046620378281766 23.95526596996951 2 15.656507152538149 19.641589267776105 39.714775480791545 24.987128098894203 3 19.493155660932416 23.45160943743326 29.027174902116705 28.02805999951762 4 13.232557357589153 15.9988348668649 35.955284909406146 34.8133228661398 5 14.581117210854961 36.783502471456345 33.444166873200146 15.191213444488556 6 33.3626107406051 34.79062450427611 17.168183901592823 14.678580853525972 7 29.955193396352254 31.64071250576025 19.92722626987674 18.47686782801076 8 27.04896255916082 34.61365437836688 19.399560809341626 18.93782225313067 9 26.92011549215173 16.623137239568013 19.58334536224316 36.8734019060371 10-11 25.72251916570616 26.139289679352203 26.77507073166988 21.363120423271763 12-13 26.40001753990099 23.150732610604148 27.26276141989153 23.186488429603333 14-15 23.18739564319056 24.708129796256774 25.286873317890183 26.81760124266248 16-17 23.231930716493963 27.960953123213024 25.847692187957307 22.959423972335706 18-19 23.188416123852893 26.55064593194785 27.92859998260657 22.332337961592682 20-21 23.839310691492017 25.8239998304356 27.817916342262077 22.518773135810303 22-23 23.50057784457123 25.682847837308202 27.627237720231868 23.189336597888698 24-25 23.375053289747665 25.906318587993916 27.744273533510917 22.97435458874751 26-27 22.962046368950787 26.33952137834733 27.55971076369735 23.13872148900454 28-29 22.891348548809578 26.51901398568689 27.759845379811964 22.829792085691565 30-31 23.000940089185136 26.08255061087525 27.67929528941317 23.23721401052644 32-33 22.8606461724375 26.139469482383472 27.55765703430709 23.44222731087194 34-35 23.532601704924165 26.19853727913748 27.30131508939743 22.96754592654092 36-37 23.466855083696846 25.814814124553305 27.40755195628842 23.31077883546143 38-39 23.04172729423392 26.24485407823719 27.434460572214626 23.278958055314263 40-41 23.3440734973574 26.01248484697915 27.496604042705396 23.146837612958052 42-43 23.26044845264401 25.884947184127764 27.341292980487065 23.513311382741165 44-45 23.08620846186629 26.20267480070884 27.312903603526088 23.39821313389878 46-47 23.231701168038537 26.40566174235338 26.88585901693013 23.476778072677952 48-49 23.21534788759565 26.325587071278665 26.886187529594707 23.572877511530972 50-51 23.339055866119768 26.455327527014582 26.83495833048668 23.37065827637897 52-53 23.631367444287 26.29652355137623 26.744814134132067 23.327294870204707 54-55 23.063912943646564 26.288321105882968 26.99301534716576 23.65475060330471 56-57 23.159205529603394 26.49587013272166 26.971338719261638 23.373585618413305 58-59 23.239837958235775 26.029118272294898 26.988652035075194 23.74239173439413 60-61 23.255205208030223 26.1582264873543 27.144477391323253 23.44209091329223 62-63 23.075329598504705 26.34524708105387 27.10646745956879 23.47295586087264 64-65 22.924044461582554 26.113407321500365 27.079307455558784 23.883240761358294 66-67 22.86926621214958 26.31944361994091 26.961874449168217 23.849415718741295 68-69 23.093102884537224 26.068277945067482 27.07340440786295 23.765214762532345 70-71 23.00109917190358 26.229617503887837 26.857627005321948 23.91165631888663 72-73 23.173944741155584 26.116435917609515 26.835618172495234 23.874001168739667 74-75 23.015386751281923 26.384414863203503 26.7046996907305 23.895498694784067 76-77 23.19277488064353 26.127016072686658 26.766116845405307 23.914092201264502 78-79 23.291648909731812 26.257862295815038 26.58133230259388 23.869156491859272 80-81 23.184007350758502 26.478148208024272 26.502608112687277 23.83523632852995 82-83 23.35877656212437 26.379319667645085 26.323731108475695 23.93817266175485 84-85 23.418516239905774 26.072443161060466 26.44618165663698 24.06285894239678 86-87 23.516641846537258 26.074323760307145 26.394084230380237 24.01495016277536 88-89 23.38980462307533 26.05489070744759 26.352592615326607 24.20271205415047 90-91 23.331530665750716 26.13090289894526 26.390505301121614 24.147061134182415 92-93 23.554197279632316 25.78657206636148 26.39041623890718 24.268814415099026 94-95 23.41303197256827 25.72075618128342 26.367891617694262 24.498320228454045 96-97 23.647593371964778 25.661174848308637 26.173581359422805 24.51765042030378 98-99 23.536154589075466 25.674574446922115 25.863550830628384 24.925720133374032 100-101 24.517790058401033 24.837574756942782 25.968232939908788 24.6764022447474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 83.5 2 95.0 3 124.5 4 113.5 5 130.0 6 216.5 7 457.5 8 738.0 9 886.5 10 929.0 11 917.0 12 1008.0 13 1180.0 14 1385.5 15 1559.0 16 1715.5 17 1955.5 18 2173.0 19 2381.0 20 2644.5 21 3037.5 22 3676.0 23 4443.5 24 5442.5 25 6901.5 26 9035.5 27 12038.0 28 15120.5 29 18512.5 30 22425.0 31 26621.0 32 31947.0 33 36665.5 34 40545.0 35 44739.0 36 50033.5 37 56233.5 38 61427.0 39 65674.0 40 71054.5 41 78695.0 42 86723.5 43 93825.0 44 98641.0 45 102598.0 46 105522.5 47 108707.5 48 114784.5 49 117320.5 50 115972.0 51 113085.5 52 109478.0 53 107286.5 54 104065.0 55 97055.0 56 87958.5 57 80330.0 58 73247.0 59 64656.0 60 54759.0 61 45513.0 62 37412.5 63 29428.0 64 23006.0 65 18388.5 66 14318.5 67 11047.0 68 8420.5 69 6408.0 70 5036.5 71 3961.5 72 3232.0 73 2686.5 74 2170.5 75 1753.5 76 1321.0 77 887.5 78 533.5 79 329.0 80 196.0 81 121.0 82 83.5 83 47.0 84 27.5 85 19.5 86 11.5 87 11.0 88 11.0 89 7.0 90 4.0 91 1.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3821113441771126 2 0.030722336563379707 3 0.036530721240641745 4 0.04580952443576475 5 0.06531692957826744 6 0.05567281917073801 7 0.036348067634438534 8 0.037443989271657784 9 0.03503296166977543 10-11 0.03740745855041715 12-13 0.029388965238096282 14-15 0.02621079249016045 16-17 0.02224720923555082 18-19 0.027617225257925156 20-21 0.03647592515878078 22-23 0.03505122703039575 24-25 0.043891661570631055 26-27 0.03811980761460966 28-29 0.03324295632898399 30-31 0.03578184145520859 32-33 0.030338763990352964 34-35 0.032073973249283454 36-37 0.034302347244962596 38-39 0.01550729116665242 40-41 0.01256656810678076 42-43 0.013370243974074878 44-45 0.010356459471721934 46-47 0.019215159372577558 48-49 0.016292701673326217 50-51 0.010557378438545463 52-53 0.00900482278581819 54-55 0.00754359393619252 56-57 0.008310739082245997 58-59 0.0037626642877860994 60-61 0.006849510232620327 62-63 0.01110533925715509 64-65 0.006666856626417118 66-67 0.003086845944834227 68-69 0.005643996431679149 70-71 0.01320585572849199 72-73 0.010429520914203218 74-75 0.01194554584568985 76-77 0.005059504891828881 78-79 0.008347269803486638 80-81 0.010575643799165785 82-83 0.012712690991743325 84-85 0.007817574345497333 86-87 0.0022101086350588255 88-89 0.016658008885732635 90-91 0.026923141554352964 92-93 0.010666970602267388 94-95 0.021607921613839592 96-97 0.019452609060641727 98-99 0.011233196781497336 100-101 0.12402179861197872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2737422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.847265643423796 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38425874748069 37.0785190547484 2 13.355637817058359 13.314840522085294 3 4.832622906561862 7.226791133336519 4 2.3658303385654325 4.717206934149695 5 1.2987961886965862 3.237071931731256 6 0.8043034896386358 2.405539782356991 7 0.5480158294197265 1.9121963438120435 8 0.38450451211004044 1.53331988449954 9 0.27842291880380315 1.2490759075347528 >10 1.554588427365125 14.179546289466346 >50 0.11732385995110393 4.051473685680127 >100 0.06985006267593961 6.414788121256216 >500 0.004439165827474936 1.446914859344009 >1k 0.0014057358453498329 1.2327155499988076 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.17823338893309107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.148059013188321 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 3.6530721240641745E-5 0.0 0.0 0.0 8 0.0 3.6530721240641745E-5 0.0 0.0 0.0 9 0.0 3.6530721240641745E-5 0.0 0.0 0.0 10-11 0.0 3.6530721240641745E-5 0.0 0.0 0.0 12-13 0.0 3.6530721240641745E-5 0.0 0.0 1.8265360620320873E-5 14-15 0.0 3.6530721240641745E-5 0.0 0.0 3.6530721240641745E-5 16-17 0.0 3.6530721240641745E-5 0.0 1.8265360620320873E-5 3.6530721240641745E-5 18-19 0.0 3.6530721240641745E-5 0.0 3.6530721240641745E-5 3.6530721240641745E-5 20-21 0.0 3.6530721240641745E-5 0.0 3.6530721240641745E-5 3.6530721240641745E-5 22-23 0.0 3.6530721240641745E-5 0.0 1.4612288496256698E-4 3.6530721240641745E-5 24-25 0.0 3.6530721240641745E-5 0.0 3.287764911657757E-4 3.6530721240641745E-5 26-27 0.0 3.6530721240641745E-5 0.0 8.950026703957227E-4 3.6530721240641745E-5 28-29 0.0 3.6530721240641745E-5 0.0 0.0037443989271657785 3.6530721240641745E-5 30-31 0.0 3.6530721240641745E-5 0.0 0.012749221712983969 3.6530721240641745E-5 32-33 0.0 3.6530721240641745E-5 0.0 0.030375294711593608 3.6530721240641745E-5 34-35 0.0 3.6530721240641745E-5 0.0 0.05983732139217117 3.6530721240641745E-5 36-37 0.0 3.6530721240641745E-5 0.0 0.10887981465773272 3.6530721240641745E-5 38-39 0.0 1.278575243422461E-4 0.0 0.18230656435142262 3.6530721240641745E-5 40-41 0.0 1.826536062032087E-4 0.0 0.26170608696795744 3.6530721240641745E-5 42-43 0.0 1.826536062032087E-4 0.0 0.34662174849182914 3.6530721240641745E-5 44-45 0.0 1.826536062032087E-4 0.0 0.45108134587944426 3.6530721240641745E-5 46-47 0.0 1.826536062032087E-4 0.0 0.5742629379028882 3.6530721240641745E-5 48-49 0.0 1.826536062032087E-4 0.0 0.7059013918935406 3.6530721240641745E-5 50-51 0.0 1.826536062032087E-4 0.0 0.8486634505019686 3.6530721240641745E-5 52-53 0.0 2.0091896682352956E-4 0.0 0.992302246420172 3.6530721240641745E-5 54-55 0.0 2.1918432744385044E-4 0.0 1.1518501714386749 3.6530721240641745E-5 56-57 0.0 2.1918432744385044E-4 0.0 1.339033587075723 3.6530721240641745E-5 58-59 0.0 2.1918432744385044E-4 0.0 1.5410119448152313 3.6530721240641745E-5 60-61 0.0 2.1918432744385044E-4 0.0 1.7589542277368997 3.6530721240641745E-5 62-63 0.0 2.1918432744385044E-4 0.0 1.9765677341674028 3.6530721240641745E-5 64-65 0.0 2.1918432744385044E-4 0.0 2.2072227080808147 3.6530721240641745E-5 66-67 0.0 2.1918432744385044E-4 0.0 2.468179915263339 3.6530721240641745E-5 68-69 0.0 2.1918432744385044E-4 0.0 2.7570831241949545 3.6530721240641745E-5 70-71 0.0 2.1918432744385044E-4 0.0 3.0689458914263126 3.6530721240641745E-5 72-73 0.0 2.1918432744385044E-4 0.0 3.3788177343500565 3.6530721240641745E-5 74-75 0.0 2.1918432744385044E-4 0.0 3.69952093612165 7.306144248128349E-5 76-77 0.0 2.3744968806417132E-4 0.0 4.046270542137822 7.306144248128349E-5 78-79 0.0 2.557150486844922E-4 0.0 4.427724333332603 7.306144248128349E-5 80-81 0.0 2.557150486844922E-4 0.0 4.82890471399733 7.306144248128349E-5 82-83 0.0 2.739804093048131E-4 0.0 5.239729205069587 7.306144248128349E-5 84-85 1.8265360620320873E-5 3.1051113054545484E-4 0.0 5.6395214183271705 7.306144248128349E-5 86-87 3.6530721240641745E-5 3.2877649116577567E-4 0.0 6.053725001114187 7.306144248128349E-5 88-89 3.6530721240641745E-5 3.2877649116577567E-4 0.0 6.5030346070134595 7.306144248128349E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4805 0.0 44.461205 1 GTATCAA 8000 0.0 37.85126 1 TATCAAC 9955 0.0 30.081245 2 ATCAACG 10015 0.0 29.806107 3 TCAACGC 10085 0.0 29.410692 4 CAACGCA 10245 0.0 28.858053 5 AACGCAG 10615 0.0 27.888786 6 GTACATG 11605 0.0 25.846521 1 TACATGG 11720 0.0 24.983292 2 ACGCAGA 12275 0.0 23.956728 7 ACATGGG 11975 0.0 23.617723 3 CGCAGAG 12530 0.0 23.54501 8 GAGTACT 5815 0.0 20.829811 12-13 CATGGGG 6780 0.0 20.541609 4 GCAGAGT 13810 0.0 20.535969 9 GGACCGA 690 0.0 19.97149 6 GTACTTT 6265 0.0 19.563433 14-15 AGTACTT 6020 0.0 19.370905 12-13 AGAGTAC 12315 0.0 18.8228 10-11 GTGGTAT 2915 0.0 18.812906 1 >>END_MODULE