FastQCFastQC Report
Thu 26 May 2016
SRR938364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737422
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.18301891341561513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42930.15682638628607498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.046.542431
GTATCAA80750.036.074171
ATCAACG100000.028.5961913
TCAACGC101650.028.1309874
CAACGCA104050.027.4821225
TATCAAC104800.027.3800962
AACGCAG106950.026.692036
GTACATG113150.023.6866111
TACATGG113050.023.364882
ACGCAGA124500.022.7386597
CGCAGAG127450.022.1378068
ACATGGG117250.021.5531393
GAGTACT62050.020.5916212-13
GCAGAGT141050.019.6665279
TGCACCG4600.019.6196695
GTACTTT65000.019.51092714-15
AGTACTT65700.018.5439412-13
GGACCGT3357.3305273E-1018.4325946
AGAGTAC128100.018.28006710-11
CATGGGG63500.018.2520644
Produced by FastQC (version 0.11.5)