##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2737422 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82428357776039 31.0 31.0 34.0 30.0 34.0 2 32.075506078346706 33.0 31.0 34.0 30.0 34.0 3 32.13788338078674 33.0 31.0 34.0 30.0 34.0 4 35.784769757823234 37.0 35.0 37.0 35.0 37.0 5 35.67408897860834 37.0 35.0 37.0 33.0 37.0 6 35.70451066733591 37.0 35.0 37.0 33.0 37.0 7 35.62516082650026 37.0 35.0 37.0 33.0 37.0 8 35.60055300205814 37.0 35.0 37.0 33.0 37.0 9 37.15029725047874 39.0 37.0 39.0 33.0 39.0 10-11 37.072398592544374 39.0 37.0 39.0 33.0 39.0 12-13 36.95020424326246 39.0 37.0 39.0 33.0 39.0 14-15 37.97795042196636 40.0 37.0 41.0 33.0 41.0 16-17 38.068071711267024 40.0 37.0 41.0 33.0 41.0 18-19 38.19297773598663 40.0 37.5 41.0 33.0 41.0 20-21 38.1418571926433 40.0 37.0 41.0 33.0 41.0 22-23 38.00952392433465 40.0 37.0 41.0 33.0 41.0 24-25 37.881301640740816 40.0 37.0 41.0 32.5 41.0 26-27 37.67059390185364 40.0 37.0 41.0 32.0 41.0 28-29 37.528743102086565 39.5 36.5 41.0 32.0 41.0 30-31 37.295808611167736 39.0 36.0 41.0 31.0 41.0 32-33 37.22975175183073 39.0 36.0 41.0 31.0 41.0 34-35 37.100680676928874 39.0 36.0 41.0 31.0 41.0 36-37 36.98343770160392 39.0 36.0 41.0 31.0 41.0 38-39 36.7803197680153 39.0 35.0 40.5 30.0 41.0 40-41 36.56852103913829 39.0 35.0 40.0 30.0 41.0 42-43 36.32054191863732 39.0 35.0 40.0 30.0 41.0 44-45 36.04398116183767 38.0 35.0 40.0 29.5 41.0 46-47 35.657362474620285 38.0 34.5 40.0 28.0 41.0 48-49 35.271875143839715 38.0 34.0 40.0 27.0 41.0 50-51 34.88256195062361 38.0 33.5 40.0 26.0 41.0 52-53 34.27981984509513 37.5 33.0 40.0 24.0 41.0 54-55 33.838821526238924 37.0 32.5 40.0 23.5 41.0 56-57 33.44316988758036 37.0 32.0 39.0 23.0 40.5 58-59 32.890928216402145 36.0 31.0 39.0 21.5 40.0 60-61 32.3762014040948 36.0 31.0 39.0 19.5 40.0 62-63 31.84367024886919 35.0 30.0 38.5 17.5 40.0 64-65 31.160263744501215 35.0 29.0 38.0 12.5 40.0 66-67 30.485376204326553 34.0 28.5 38.0 8.0 40.0 68-69 29.737540284252848 34.0 28.0 37.0 7.0 39.0 70-71 28.935654970260337 33.5 26.0 36.0 2.0 39.0 72-73 28.33349169400991 33.0 26.0 36.0 2.0 38.5 74-75 27.595233763738293 32.0 25.5 35.0 2.0 38.0 76-77 26.43420652716315 30.5 24.0 34.0 2.0 36.0 78-79 26.92688065632555 32.0 25.0 35.0 2.0 36.0 80-81 26.531030107889833 32.0 24.5 35.0 2.0 36.0 82-83 25.833378996734883 31.5 22.0 34.0 2.0 36.0 84-85 25.039325138761946 31.0 19.5 34.0 2.0 35.0 86-87 24.107324884508124 30.5 15.0 34.0 2.0 35.0 88-89 23.120900431135574 29.5 7.0 34.0 2.0 35.0 90-91 22.237252970130292 29.0 2.0 34.0 2.0 35.0 92-93 21.129912742719245 29.0 2.0 33.5 2.0 35.0 94-95 19.93325892025417 26.5 2.0 33.0 2.0 34.0 96-97 18.523673733899997 25.0 2.0 32.5 2.0 34.0 98-99 16.730228660396534 11.5 2.0 32.0 2.0 34.0 100-101 12.484404487141552 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.345221368431023 1101 2 0.3535576873327102 1101 3 0.3806528060582437 1101 4 0.21446447833789506 1101 5 0.18942104435333107 1101 6 0.2348294632097847 1101 7 0.30725006200820104 1101 8 0.30257584347306477 1101 9 0.35490480752982023 1101 10-11 0.3975382064140902 1101 12-13 0.45375814546745374 1101 14-15 0.5611700244604805 1101 16-17 0.5024406171636642 1101 18-19 0.48992873907589285 1101 20-21 0.584742048740793 1101 22-23 0.4946923744756262 1101 24-25 0.4099398747296874 1101 26-27 0.6033608107300452 1101 28-29 0.6637549914175978 1101 30-31 0.6181160158149268 1101 32-33 0.7383448844484022 1101 34-35 0.4908081596517917 1101 36-37 0.4390636703161235 1101 38-39 0.5956457936817543 1101 40-41 0.7224939767415748 1101 42-43 0.7844903860617833 1101 44-45 0.8145248711088158 1101 46-47 0.8190648223429378 1101 48-49 0.8726042341510549 1101 50-51 1.0153993190437518 1101 52-53 1.0465438046390858 1101 54-55 1.1093837777617992 1101 56-57 1.3225888162714483 1101 58-59 1.5724341973818667 1101 60-61 1.4167921983843001 1101 62-63 1.5763798818834651 1101 64-65 1.6322972763071846 1101 66-67 1.7947127529637648 1101 68-69 1.8888917375966408 1101 70-71 2.10518610980726 1101 72-73 2.284586851517865 1101 74-75 2.187038423466806 1101 76-77 2.0781164901739615 1101 78-79 2.1245646937497256 1101 80-81 2.3508663709267594 1101 82-83 2.4548155789241903 1101 84-85 2.7573777917465527 1101 86-87 2.7798962107499356 1101 88-89 2.553196167354468 1101 90-91 2.8826815072607666 1101 92-93 3.1528125604220385 1101 94-95 3.1384095984177627 1101 96-97 3.0891505418465 1101 98-99 3.327917404548895 1101 100-101 2.846234307297646 1102 1 0.29327095031323225 1102 2 0.2223789870661932 1102 3 0.19092217948064416 1102 4 0.1969120121247343 1102 5 0.21049600163296134 1102 6 0.20262628563228446 1102 7 0.2491764574275166 1102 8 0.2301605430970426 1102 9 0.3127988107342219 1102 10-11 0.3679688216780832 1102 12-13 0.2996694891871243 1102 14-15 0.5361370582913167 1102 16-17 0.3665902443479965 1102 18-19 0.3694759182904477 1102 20-21 0.24055216997132334 1102 22-23 0.43041872734562503 1102 24-25 0.30085061617133135 1102 26-27 0.332840593680821 1102 28-29 0.22011357550253052 1102 30-31 0.25009454448392177 1102 32-33 0.3087731447018882 1102 34-35 0.4419504431813692 1102 36-37 0.3642566021657814 1102 38-39 0.4462108291270468 1102 40-41 0.4018931880994785 1102 42-43 0.4366004357031841 1102 44-45 0.48410424554482034 1102 46-47 0.41992347827160614 1102 48-49 0.5922991075318507 1102 50-51 0.7022426709047593 1102 52-53 0.7629849445723806 1102 54-55 0.6668264622955675 1102 56-57 0.8645599568905524 1102 58-59 1.069363295493993 1102 60-61 1.0914283214132325 1102 62-63 1.1985916409283384 1102 64-65 1.0129058810382432 1102 66-67 1.3297838718945023 1102 68-69 1.5519357987295308 1102 70-71 1.7749169687034652 1102 72-73 1.7039578950405314 1102 74-75 1.776909443678047 1102 76-77 1.7152115974327558 1102 78-79 1.8710766924487388 1102 80-81 1.9621892296612913 1102 82-83 2.171555630158398 1102 84-85 2.2988982979210597 1102 86-87 2.338217568801756 1102 88-89 2.014975301420286 1102 90-91 2.3048307471413416 1102 92-93 2.572081288285137 1102 94-95 2.557676098568212 1102 96-97 2.404264633354252 1102 98-99 2.2231028878821846 1102 100-101 2.0240780978589203 1103 1 0.17637667277643843 1103 2 0.15109721225984885 1103 3 0.14271165351156867 1103 4 0.08245151928034034 1103 5 0.11651741792018555 1103 6 0.12308437493463487 1103 7 0.18515875739297627 1103 8 0.1905371329508725 1103 9 0.18254656458121588 1103 10-11 0.23318414559856393 1103 12-13 0.3404596367907189 1103 14-15 0.39475664004745425 1103 16-17 0.3213223131892491 1103 18-19 0.3535362454889608 1103 20-21 0.3364586477222389 1103 22-23 0.301361964138529 1103 24-25 0.24587546829301488 1103 26-27 0.3131062918241483 1103 28-29 0.287889748157518 1103 30-31 0.13043029478383517 1103 32-33 0.1623502886848982 1103 34-35 0.2624484168490042 1103 36-37 0.279118903041514 1103 38-39 0.2954507729595619 1103 40-41 0.37750271597128915 1103 42-43 0.33719756334019024 1103 44-45 0.2907156779549709 1103 46-47 0.4287704263615879 1103 48-49 0.44413894605406057 1103 50-51 0.4119232681042533 1103 52-53 0.5128248606475267 1103 54-55 0.5865566319882731 1103 56-57 0.6203793088624465 1103 58-59 0.7941051063609521 1103 60-61 0.7269525200990543 1103 62-63 0.7813470576937611 1103 64-65 0.9772825852751623 1103 66-67 0.9860205424842299 1103 68-69 0.897478204388559 1103 70-71 1.021409532530054 1103 72-73 1.299608208568472 1103 74-75 1.1863777734109568 1103 76-77 1.2436470239117554 1103 78-79 1.2963138689291256 1103 80-81 1.5502180212623387 1103 82-83 1.6543606628346836 1103 84-85 1.7189777552421681 1103 86-87 1.6154350174929313 1103 88-89 1.5801399763889066 1103 90-91 1.8551593814356941 1103 92-93 1.9883751743742515 1103 94-95 1.5488042227499221 1103 96-97 1.7922766268104589 1103 98-99 1.6088275938360468 1103 100-101 1.7547312986615804 1104 1 0.06856870172600438 1104 2 0.12595283145559932 1104 3 0.13296855313839018 1104 4 0.072470346814967 1104 5 0.024585879518163267 1104 6 0.015279379785354763 1104 7 0.04135626993639363 1104 8 0.10278871960217373 1104 9 0.053943988244391505 1104 10-11 0.17936894112440172 1104 12-13 0.08424545920083659 1104 14-15 0.2214599962687771 1104 16-17 0.20970717259775995 1104 18-19 0.216080859207068 1104 20-21 0.2686066134954075 1104 22-23 0.33549555035732936 1104 24-25 0.22926845191166478 1104 26-27 0.29054796912139835 1104 28-29 0.3190538810096726 1104 30-31 0.402176196471963 1104 32-33 0.46534563239156057 1104 34-35 0.4851842024571553 1104 36-37 0.3938418764032292 1104 38-39 0.4653166986929733 1104 40-41 0.48802818075745336 1104 42-43 0.3909140670928011 1104 44-45 0.5393537071942021 1104 46-47 0.6190187950717743 1104 48-49 0.6375055631885047 1104 50-51 0.6735892966054564 1104 52-53 0.6112696547052536 1104 54-55 0.7432656027431008 1104 56-57 0.7757273008235401 1104 58-59 0.8855922435242078 1104 60-61 0.942898291244326 1104 62-63 0.9461227045058784 1104 64-65 1.1992669558423046 1104 66-67 1.1132790034671878 1104 68-69 1.1528293706442234 1104 70-71 1.0138938941694953 1104 72-73 1.4817849413861133 1104 74-75 1.4094076772496713 1104 76-77 1.5349083893742375 1104 78-79 1.8088319971269726 1104 80-81 1.8325129965524383 1104 82-83 1.9243656623486167 1104 84-85 2.1164480408691624 1104 86-87 2.197176574365379 1104 88-89 2.165833529991829 1104 90-91 2.3394590229388044 1104 92-93 2.376418797117278 1104 94-95 2.2063573574649986 1104 96-97 2.2631623938688215 1104 98-99 2.544216811947777 1104 100-101 2.1193342440101492 1105 1 0.15878657652677575 1105 2 0.1672970371375655 1105 3 0.1280467509556118 1105 4 0.09072731105165133 1105 5 0.030045891242963307 1105 6 0.011363551808543093 1105 7 0.11642049084939998 1105 8 0.07405134531225599 1105 9 0.2019251954721426 1105 10-11 0.18142425049348532 1105 12-13 0.18518069500839118 1105 14-15 0.2414329870621117 1105 16-17 0.06430934448806624 1105 18-19 0.2029360184004858 1105 20-21 0.21039412826147696 1105 22-23 0.2112422593941048 1105 24-25 0.28886742042787716 1105 26-27 0.23830748845250582 1105 28-29 0.29258975493042527 1105 30-31 0.34058635963991435 1105 32-33 0.39015586483029807 1105 34-35 0.3609261751432129 1105 36-37 0.3512727570362557 1105 38-39 0.3680446632549277 1105 40-41 0.4762325995191645 1105 42-43 0.48455112013805746 1105 44-45 0.32535944524972393 1105 46-47 0.2925414739197194 1105 48-49 0.30974949405540286 1105 50-51 0.4965517118614642 1105 52-53 0.5211841308875478 1105 54-55 0.5301530358304731 1105 56-57 0.7960195616798629 1105 58-59 0.8916220849351717 1105 60-61 0.82963326720634 1105 62-63 0.95952404205131 1105 64-65 1.144772906166395 1105 66-67 1.150914446420046 1105 68-69 1.3696017357589554 1105 70-71 1.2766424158276912 1105 72-73 1.276745517603871 1105 74-75 1.2933258587258152 1105 76-77 1.3166412052935001 1105 78-79 1.5958974769753382 1105 80-81 1.7253060319021607 1105 82-83 1.8286271371921927 1105 84-85 1.8235292987691913 1105 86-87 2.0606938257556635 1105 88-89 2.0045292350690147 1105 90-91 2.0830257244988104 1105 92-93 2.1257443007514603 1105 94-95 2.1552689440731108 1105 96-97 2.4503201591758383 1105 98-99 2.197496327884732 1105 100-101 2.203286593920531 1106 1 0.060088759144075965 1106 2 0.058433646860713395 1106 3 0.0299542914106965 1106 4 0.048292823563080844 1106 5 0.044436724089585766 1106 6 -0.02354975357663136 1106 7 0.020416936763687943 1106 8 0.02079401376685297 1106 9 -0.09405141980713694 1106 10-11 0.08187413621401873 1106 12-13 -0.0054205442022094985 1106 14-15 0.004885394086556971 1106 16-17 0.03386632484684782 1106 18-19 0.05954982063572345 1106 20-21 0.08567013304261195 1106 22-23 0.15757185339491286 1106 24-25 0.047751646187833785 1106 26-27 -0.07023004685843404 1106 28-29 0.1663625692104418 1106 30-31 0.261892264200263 1106 32-33 0.08136667106720097 1106 34-35 -0.041539656769089106 1106 36-37 0.1156686600417558 1106 38-39 0.14080543555683533 1106 40-41 0.12293531894830778 1106 42-43 0.2110041224438035 1106 44-45 0.1899605653275387 1106 46-47 -0.07079631562621813 1106 48-49 0.1133084522225758 1106 50-51 0.2749837279186309 1106 52-53 0.17252799084860726 1106 54-55 0.049540302984226514 1106 56-57 0.12422816549075577 1106 58-59 0.5247152861901228 1106 60-61 0.6041154062802221 1106 62-63 0.5491992725458381 1106 64-65 0.7373175277099691 1106 66-67 0.744176543277046 1106 68-69 0.6484902574422087 1106 70-71 0.5155430727289563 1106 72-73 0.7097181447408119 1106 74-75 0.7229664667316165 1106 76-77 0.6997285073069861 1106 78-79 0.8315384362570519 1106 80-81 1.0202968679885807 1106 82-83 1.1627518192230788 1106 84-85 1.3504146612651446 1106 86-87 1.3645402204772914 1106 88-89 1.592141006562116 1106 90-91 1.4731605473576614 1106 92-93 1.416883538028646 1106 94-95 1.2458412419239586 1106 96-97 1.4647008517046949 1106 98-99 1.5923401484962625 1106 100-101 1.678014689909416 1107 1 0.09389571621432324 1107 2 0.09117075253657703 1107 3 0.1379773093879919 1107 4 0.1161761523834528 1107 5 0.07541879264657325 1107 6 -0.004725053430583159 1107 7 0.02380345698311004 1107 8 0.1349861817804836 1107 9 0.16295469407199903 1107 10-11 0.16277704716899422 1107 12-13 0.16329488437346384 1107 14-15 0.26038946535876306 1107 16-17 0.12682975620342063 1107 18-19 0.0833634015397493 1107 20-21 0.19242458461209822 1107 22-23 0.19186168732814934 1107 24-25 0.16580949351001095 1107 26-27 0.2833865106192732 1107 28-29 0.1549646800385176 1107 30-31 0.15126259103595885 1107 32-33 0.3365473579754905 1107 34-35 0.21454537863341017 1107 36-37 0.1439788264802928 1107 38-39 0.220744017932482 1107 40-41 0.20275741107597867 1107 42-43 0.14007383008425478 1107 44-45 0.1424174871718762 1107 46-47 0.4155903117890176 1107 48-49 0.31214918836658256 1107 50-51 0.37191297062667417 1107 52-53 0.5101893499799814 1107 54-55 0.41718092313369226 1107 56-57 0.5112733965473026 1107 58-59 0.555720404945113 1107 60-61 0.6146375118538501 1107 62-63 0.7824372243250686 1107 64-65 0.9396614602100613 1107 66-67 0.8302307397452857 1107 68-69 0.9517393439541841 1107 70-71 0.9205002284832382 1107 72-73 1.210136394301344 1107 74-75 1.210091196186113 1107 76-77 1.1476865509895902 1107 78-79 1.1760709850574784 1107 80-81 1.4238428187441876 1107 82-83 1.694552479623642 1107 84-85 1.8817462005660417 1107 86-87 2.1545478976803345 1107 88-89 2.0814726065866545 1107 90-91 2.3683531559275046 1107 92-93 2.482419712278226 1107 94-95 2.4098548018845243 1107 96-97 2.6609920624535235 1107 98-99 2.774272201221727 1107 100-101 2.4392896137105424 1108 1 0.00616520949663979 1108 2 0.040695943701408055 1108 3 0.051679877038395716 1108 4 0.06337748050688674 1108 5 0.006515918144124555 1108 6 0.07870399468095002 1108 7 0.005854612325784103 1108 8 -0.029909496759046306 1108 9 0.09353318313156933 1108 10-11 0.06477788018759867 1108 12-13 0.07005104171044962 1108 14-15 0.13559327841903723 1108 16-17 0.177249206952375 1108 18-19 -0.04752670471718545 1108 20-21 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-0.49187954053032 2316 60-61 -0.33083815197700517 2316 62-63 -0.3680382619573024 2316 64-65 -0.2534948477437631 2316 66-67 -0.2059075922841167 2316 68-69 0.09399496565450605 2316 70-71 0.09710145803509107 2316 72-73 0.3905949504771087 2316 74-75 0.45758747625910345 2316 76-77 0.3450381004150742 2316 78-79 0.040787806687696104 2316 80-81 -0.3279231586143716 2316 82-83 0.1915142242264558 2316 84-85 0.3290793078591605 2316 86-87 0.40656209926773457 2316 88-89 0.1818437401821491 2316 90-91 0.285951303008666 2316 92-93 0.4060060541037238 2316 94-95 0.669382740569592 2316 96-97 0.5272543004120109 2316 98-99 0.3693771896467126 2316 100-101 0.42395028420232883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 15.0 4 46.0 5 116.0 6 198.0 7 520.0 8 1217.0 9 2169.0 10 3509.0 11 5402.0 12 7771.0 13 11287.0 14 15187.0 15 19939.0 16 24773.0 17 30910.0 18 32273.0 19 31540.0 20 33739.0 21 37108.0 22 41150.0 23 46025.0 24 52142.0 25 59355.0 26 67905.0 27 79139.0 28 93563.0 29 109636.0 30 127243.0 31 147296.0 32 172505.0 33 205706.0 34 247176.0 35 297792.0 36 342544.0 37 297215.0 38 91827.0 39 1475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57835723407871 19.014420837040706 14.615599644092352 23.791622284788236 2 16.17867183518232 20.3141663485058 38.6075331968134 24.899628619498483 3 19.560441326785956 23.67247245726026 29.409486874685374 27.357599341268408 4 13.767625159730578 16.683653452043565 35.634914894378724 33.91380649384713 5 14.538434646142179 36.82034826312288 33.655644946524326 14.98557214421061 6 33.397189034061974 35.19720379247336 16.865284198052034 14.540322975412634 7 29.886659784278784 31.719369538200542 19.90489592032211 18.489074757198562 8 27.015328301831655 34.95488452630579 19.240270035288702 18.789517136573856 9 26.63258335433859 16.451390182690936 19.495106688439073 37.420919774531406 10-11 25.68133448185921 26.257149975414823 26.773657843036258 21.28785769968971 12-13 26.246373412648836 23.14018445091769 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23.20411633730732 38-39 23.06439989368222 26.263268029494963 27.48212281164856 23.19020926517426 40-41 23.28365761384831 26.002389403325072 27.58164251384228 23.132310468984336 42-43 23.222100751383902 25.914168522978535 27.412241885884995 23.451488839752564 44-45 23.097367762534223 26.223156602694885 27.353131889508493 23.326343745262392 46-47 23.269438643160846 26.37199393151437 26.947407797339174 23.41115962798561 48-49 23.19432297979632 26.317005562167616 27.014084784881543 23.474586673154523 50-51 23.300426459639763 26.408752468563492 26.91684000493895 23.373981066857795 52-53 23.687122409332577 26.291050484726142 26.737382836844304 23.284444269096984 54-55 23.123380319146992 26.282758011004514 26.97810202445951 23.615759645388984 56-57 23.14035306583221 26.501033910926765 26.974380817860894 23.384232205380137 58-59 23.26703738042582 26.092250299734566 26.966686174071807 23.674026145767808 60-61 23.216533658310627 26.269990523930908 27.10902812938597 23.40444768837249 62-63 23.047670399375765 26.398925704549754 27.012039064492065 23.541364831582413 64-65 22.88721184312452 26.17441353069926 26.98556067128184 23.95281395489438 66-67 22.80430977041055 26.362073172870105 26.900447430562053 23.933169626157298 68-69 23.022840166326166 26.093755279887702 27.000768066691204 23.882636487094928 70-71 22.82782276234141 26.265073268794474 26.839354786741755 24.067749182122366 72-73 23.00645937628771 26.178940285290945 26.71513938692548 24.099460951495864 74-75 22.81000152698415 26.405318580766867 26.594931289366418 24.189748602882567 76-77 22.842897441461346 26.128707959532726 26.952183477739272 24.076211121266653 78-79 22.881930517106973 26.221276807156514 26.816764094100215 24.0800285816363 80-81 22.667642038527188 26.418239088483947 26.830269770687153 24.083849102301713 82-83 22.821076242193765 26.316753701968477 26.696220311646655 24.1659497441911 84-85 22.67817217343017 26.068959974280663 26.817889552980372 24.434978299308792 86-87 22.769102906591776 26.06646117313971 26.75584172431902 24.408594195949497 88-89 22.519728416006007 26.167832361981457 26.599424568079016 24.71301465393352 90-91 22.42267932961506 26.05602249594275 26.91244281718028 24.60885535726191 92-93 22.478300156224627 25.80225173152159 26.894015846032254 24.825432266221526 94-95 22.284665435945993 25.71263713411951 26.894187429370092 25.1085100005644 96-97 22.38661046780511 25.655251546893393 26.781311029136173 25.17682695616533 98-99 22.182677716479226 25.73222177654742 26.581177472819316 25.50392303415403 100-101 22.269645238818025 25.339836539455597 26.863132753882397 25.52738546784398 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 128.0 1 93.0 2 88.0 3 121.5 4 117.0 5 132.5 6 232.0 7 480.5 8 776.5 9 961.0 10 961.5 11 937.0 12 1062.5 13 1206.0 14 1389.0 15 1612.0 16 1807.5 17 1970.5 18 2145.0 19 2333.5 20 2604.0 21 3018.0 22 3617.0 23 4368.0 24 5327.5 25 6688.5 26 8780.5 27 11946.0 28 15000.0 29 18276.5 30 22372.0 31 26879.5 32 32473.0 33 36720.5 34 40481.5 35 45195.0 36 50790.0 37 56931.0 38 62068.5 39 66345.0 40 71437.0 41 79390.5 42 87469.5 43 95036.5 44 100415.0 45 104226.0 46 107013.0 47 110745.0 48 117409.5 49 119378.0 50 117418.5 51 114212.5 52 109629.5 53 107416.0 54 105260.0 55 97376.5 56 87276.5 57 79373.0 58 72218.5 59 63679.5 60 53398.0 61 43924.0 62 35769.0 63 27377.5 64 20787.0 65 16365.0 66 12440.0 67 9790.5 68 7575.0 69 5533.0 70 4349.0 71 3486.0 72 2909.0 73 2431.0 74 1950.0 75 1698.5 76 1208.5 77 670.0 78 400.0 79 268.0 80 158.0 81 80.0 82 42.5 83 24.5 84 12.5 85 6.5 86 5.5 87 5.5 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0276537559791658 2 0.01231085305809627 3 0.0014612288496256697 4 0.0 5 3.2877649116577567E-4 6 0.0 7 0.0 8 7.306144248128349E-5 9 4.3836865488770094E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001716943898310162 26-27 0.008676046294652413 28-29 2.009189668235296E-4 30-31 0.0 32-33 3.835725730267383E-4 34-35 0.009571048965048137 36-37 0.0013516366859037444 38-39 0.01258483346740108 40-41 0.01231085305809627 42-43 0.005096035613069524 44-45 6.210222610909096E-4 46-47 3.6530721240641745E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.8265360620320873E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.132680310160436E-5 66-67 5.114300973689844E-4 68-69 0.0010776562765989314 70-71 5.479608186096262E-5 72-73 2.1918432744385047E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6438824558288783E-4 82-83 9.86329473497327E-4 84-85 0.006648591265796797 86-87 0.004000113975850271 88-89 0.0 90-91 0.0014429634890053488 92-93 6.392876217112305E-4 94-95 5.479608186096262E-5 96-97 0.0 98-99 0.0 100-101 1.0959216372192524E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2737422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.71791902572477 #Duplication Level Percentage of deduplicated Percentage of total 1 71.58143758456235 33.44135804820556 2 14.709550051931346 13.743991364619484 3 5.365598068464196 7.520087282612867 4 2.6127873040111322 4.882559428009352 5 1.4779924467380077 3.4524365723669534 6 0.8861049232869339 2.4838186832649023 7 0.6253043116678121 2.044904133925338 8 0.4280430679699471 1.5997825111154236 9 0.3333613864810463 1.4016555233932375 >10 1.7545989630935062 15.004559784323664 >50 0.13745026317194156 4.457860545140646 >100 0.08086376227973563 7.006056332597428 >500 0.005102401275468044 1.5158358656650095 >1k 0.0017269665855254642 1.2613629118671572 >5k 7.849848116024837E-5 0.18373101289299193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5010 0.18301891341561513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4293 0.15682638628607498 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.0959216372192524E-4 0.0 24-25 0.0 1.8265360620320873E-5 0.0 2.9224576992513396E-4 0.0 26-27 0.0 3.6530721240641745E-5 0.0 8.584719491550809E-4 0.0 28-29 0.0 3.6530721240641745E-5 0.0 0.0037078682059251368 0.0 30-31 0.0 3.6530721240641745E-5 0.0 0.01280401779484493 0.0 32-33 0.0 3.6530721240641745E-5 0.0 0.030503152235935853 0.0 34-35 0.0 3.6530721240641745E-5 0.0 0.06016609788333695 0.0 36-37 0.0 3.6530721240641745E-5 0.0 0.10950083691882362 0.0 38-39 0.0 5.479608186096262E-5 0.0 0.18314677093995738 0.0 40-41 0.0 7.306144248128349E-5 0.0 0.2631490504569628 0.0 42-43 0.0 7.306144248128349E-5 0.0 0.3480464466202142 0.0 44-45 1.8265360620320873E-5 7.306144248128349E-5 0.0 0.45323665843264205 0.0 46-47 5.479608186096262E-5 1.0959216372192522E-4 0.0 0.5765278426198079 0.0 48-49 7.306144248128349E-5 1.0959216372192522E-4 0.0 0.708367215577284 0.0 50-51 7.306144248128349E-5 1.0959216372192522E-4 0.0 0.8507822323339258 0.0 52-53 7.306144248128349E-5 1.0959216372192522E-4 0.0 0.9940009249578619 0.0 54-55 7.306144248128349E-5 1.0959216372192522E-4 0.0 1.1531652774033379 0.0 56-57 7.306144248128349E-5 1.0959216372192522E-4 0.0 1.339143179239445 0.0 58-59 7.306144248128349E-5 1.0959216372192522E-4 0.0 1.5400438807023544 0.0 60-61 7.306144248128349E-5 1.0959216372192522E-4 0.0 1.7565432001350176 0.0 62-63 7.306144248128349E-5 1.0959216372192522E-4 0.0 1.974357625532344 0.0 64-65 7.306144248128349E-5 1.0959216372192522E-4 0.0 2.2047020883152104 0.0 66-67 7.306144248128349E-5 1.0959216372192522E-4 0.0 2.4672849125929432 0.0 68-69 7.306144248128349E-5 1.0959216372192522E-4 0.0 2.7582521072746546 0.0 70-71 7.306144248128349E-5 1.826536062032087E-4 0.0 3.0734757008601523 0.0 72-73 7.306144248128349E-5 1.826536062032087E-4 0.0 3.389429908870463 0.0 74-75 7.306144248128349E-5 1.826536062032087E-4 0.0 3.71771323529949 0.0 76-77 7.306144248128349E-5 1.826536062032087E-4 0.0 4.072828376479768 0.0 78-79 7.306144248128349E-5 1.826536062032087E-4 0.0 4.464437708179448 0.0 80-81 7.306144248128349E-5 1.826536062032087E-4 0.0 4.878257718393437 0.0 82-83 7.306144248128349E-5 1.826536062032087E-4 0.0 5.306909201431128 0.0 84-85 7.306144248128349E-5 1.826536062032087E-4 0.0 5.724455345211663 0.0 86-87 7.306144248128349E-5 1.826536062032087E-4 0.0 6.158440313550487 0.0 88-89 7.306144248128349E-5 1.826536062032087E-4 0.0 6.638709705701203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4860 0.0 46.54243 1 GTATCAA 8075 0.0 36.07417 1 ATCAACG 10000 0.0 28.596191 3 TCAACGC 10165 0.0 28.130987 4 CAACGCA 10405 0.0 27.482122 5 TATCAAC 10480 0.0 27.380096 2 AACGCAG 10695 0.0 26.69203 6 GTACATG 11315 0.0 23.686611 1 TACATGG 11305 0.0 23.36488 2 ACGCAGA 12450 0.0 22.738659 7 CGCAGAG 12745 0.0 22.137806 8 ACATGGG 11725 0.0 21.553139 3 GAGTACT 6205 0.0 20.59162 12-13 GCAGAGT 14105 0.0 19.666527 9 TGCACCG 460 0.0 19.619669 5 GTACTTT 6500 0.0 19.510927 14-15 AGTACTT 6570 0.0 18.54394 12-13 GGACCGT 335 7.3305273E-10 18.432594 6 AGAGTAC 12810 0.0 18.280067 10-11 CATGGGG 6350 0.0 18.252064 4 >>END_MODULE