FastQCFastQC Report
Thu 26 May 2016
SRR938363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146056
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25531.747959686695514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24451.6740154461302514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16631.1386043709262201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11780.806539957276661No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.44024209892096183No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6360.4354494166621022No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5800.3971079585912253No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5580.3820452429205236No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA4960.3395957714849099No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4790.32795640028482226No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4670.3197403735553486No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4430.3033083200964014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.29851563783754176No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4290.29372295557868217No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4290.29372295557868217No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3900.2670208687078929No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA3800.26017417976666485No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3790.259489510872542No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA3640.24921947746070003No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA3580.2451114640959632No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.2410034507312264No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3440.23552609957824397No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT3350.22936407953113874No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT3290.22525606616640192No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3040.20813934381333188No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT2970.20334666155447226No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT2960.20266199266034943No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT2960.20266199266034943No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT2960.20266199266034943No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC2940.20129265487210385No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT2910.19923864818973547No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2750.1882839458837706No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT2740.18759927698964782No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC2730.18691460809552501No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2670.1828065947307882No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA2520.17253656131894615No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2470.16911321684833214No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2430.16637454127184095No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG2380.1629511968012269No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.16089719011885853No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2350.16089719011885853No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.1602125212247357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2240.1533658322835077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2240.1533658322835077No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2180.14925781891877088No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA2180.14925781891877088No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.14720381223640247No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA2140.14651914334227967No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC2140.14651914334227967No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG2110.1444651366599113No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2020.13830311661280603No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC2000.13693377882456045No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA1990.13624910993043762No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT1980.13556444103631485No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT1890.12940242098920962No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA1830.1252944076244728No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA1810.1239250698362272No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC1790.1225557320479816No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA1790.1225557320479816No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1780.12187106315385879No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA1780.12187106315385879No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA1720.11776304978912198No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC1710.11707838089499917No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT1680.11502437421263077No Hit
GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT1660.11365503642438517No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT1660.11365503642438517No Hit
GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA1650.11297036753026236No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC1630.11160102974201677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1620.11091636084789395No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1620.11091636084789395No Hit
AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT1600.10954702305964836No Hit
GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG1590.10886235416552555No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG1580.10817768527140274No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC1570.10749301637727995No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC1550.10612367858903433No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA1550.10612367858903433No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT1540.10543900969491155No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA1530.10475434080078874No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT1530.10475434080078874No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA1530.10475434080078874No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT1520.10406967190666594No Hit
CCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT1510.10338500301254315No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC1490.10201566522429753No Hit
GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC1490.10201566522429753No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA1470.10064632743605192No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAA156.602091E-495.015079
GACGTAT156.602091E-495.015076
ATGGGTT200.002063516871.3101655
GGTACCA200.00206913771.261318
CTGGGCT451.5018701E-552.8223424
GGTATCA15050.051.172741
TGGGTAC405.368438E-447.5075386
AGGTTAG459.557631E-442.2578741
GAGTACC500.00160452638.032091
AGTGTTT250.001612583938.0060374-75
GTATCAA21950.035.086552
GTTAGAG550.002561652334.5746273
GGTTAGA550.002561652334.5746272
GGGTGAG1001.2873206E-633.2552768
GGTGAGA1001.2873206E-633.2552769
CTAGATG753.050245E-431.6934054
ATGGGAT1102.6941452E-630.25285
ATGGGGT1751.4733814E-1029.8823555
GTGGTAT6700.029.8012621
CACAAGA404.4813557E-429.69221192-93
Produced by FastQC (version 0.11.5)