##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938363_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146056 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.685305636194336 31.0 31.0 34.0 27.0 34.0 2 30.68906446842307 31.0 31.0 34.0 27.0 34.0 3 30.6162978583557 31.0 31.0 34.0 27.0 34.0 4 34.262310346716326 37.0 35.0 37.0 31.0 37.0 5 34.29336692775374 37.0 35.0 37.0 30.0 37.0 6 34.38057320479816 37.0 35.0 37.0 31.0 37.0 7 34.33450868160158 37.0 35.0 37.0 30.0 37.0 8 34.32304732431396 37.0 35.0 37.0 30.0 37.0 9 35.71849153749247 39.0 35.0 39.0 30.0 39.0 10-11 35.60687681437257 39.0 35.0 39.0 30.0 39.0 12-13 35.503426767815085 39.0 35.0 39.0 30.0 39.0 14-15 36.33380004929616 39.0 36.0 41.0 29.5 41.0 16-17 36.20091266363586 39.0 36.0 41.0 29.5 41.0 18-19 36.48422865202388 39.0 36.0 41.0 30.0 41.0 20-21 36.60450443665444 39.5 36.0 41.0 30.5 41.0 22-23 36.48315029851564 40.0 36.0 41.0 30.0 41.0 24-25 36.33576504902229 39.0 36.0 41.0 30.0 41.0 26-27 36.097873418414856 39.0 35.5 41.0 29.5 41.0 28-29 35.824344771868326 39.0 35.0 41.0 28.5 41.0 30-31 35.35282699786383 38.5 35.0 40.0 27.0 41.0 32-33 34.84160185134469 38.0 35.0 40.0 26.5 41.0 34-35 34.45651667853426 38.0 35.0 40.0 24.5 41.0 36-37 34.06118543572329 38.0 34.0 40.0 23.0 41.0 38-39 33.58691186941995 37.5 33.0 40.0 18.5 41.0 40-41 33.28612655419839 38.0 33.0 40.0 12.5 41.0 42-43 32.691197212028264 37.5 33.0 40.0 2.0 41.0 44-45 31.86329900859944 37.0 32.0 40.0 2.0 41.0 46-47 30.918866736046446 37.0 30.5 40.0 2.0 41.0 48-49 30.118006107246536 37.0 28.5 40.0 2.0 41.0 50-51 29.243687352796186 36.0 26.0 39.5 2.0 40.5 52-53 29.13410609629183 36.0 26.0 39.5 2.0 40.0 54-55 29.097678287780028 36.5 24.5 40.0 2.0 41.0 56-57 28.74268773621077 36.0 22.5 40.0 2.0 41.0 58-59 28.337832749082544 36.0 20.5 40.0 2.0 41.0 60-61 27.913666675795586 35.5 16.0 39.0 2.0 41.0 62-63 27.355894314509506 35.0 7.5 39.0 2.0 40.0 64-65 26.86206318124555 35.0 2.0 39.0 2.0 40.0 66-67 26.359865394095415 34.5 2.0 38.0 2.0 40.0 68-69 25.769920441474504 34.0 2.0 37.5 2.0 39.5 70-71 25.220477077285423 33.5 2.0 37.0 2.0 39.0 72-73 24.65125362874514 33.0 2.0 36.0 2.0 39.0 74-75 24.057864791586788 32.5 2.0 36.0 2.0 38.0 76-77 23.431118885906777 31.5 2.0 35.0 2.0 37.5 78-79 22.799884975625787 31.0 2.0 35.0 2.0 37.0 80-81 22.187756750835298 31.0 2.0 35.0 2.0 36.0 82-83 21.554828285041353 30.0 2.0 34.5 2.0 36.0 84-85 21.0220874185244 29.0 2.0 34.0 2.0 35.0 86-87 20.42422084679849 29.0 2.0 34.0 2.0 35.0 88-89 19.718152626389877 27.5 2.0 34.0 2.0 35.0 90-91 19.03023497836446 26.0 2.0 34.0 2.0 35.0 92-93 18.32304732431396 25.0 2.0 34.0 2.0 35.0 94-95 17.50971887495207 21.5 2.0 33.0 2.0 35.0 96-97 16.53323382812072 10.0 2.0 32.5 2.0 35.0 98-99 15.337079613299009 2.0 2.0 32.0 2.0 34.0 100-101 12.360998521115189 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3973242409669417 1101 2 1.2133930344689752 1101 3 1.1504546265977282 1101 4 1.0251016891730558 1101 5 0.8809272172219735 1101 6 1.3975472096234185 1101 7 0.9718792371587242 1101 8 1.1340307847654216 1101 9 1.1514853056386656 1101 10-11 1.3034082336982138 1101 12-13 1.3734635652567206 1101 14-15 1.7444639494384901 1101 16-17 1.670770274296821 1101 18-19 1.427831325269942 1101 20-21 0.9147927762292127 1101 22-23 1.261294801770383 1101 24-25 0.824974683427179 1101 26-27 0.8841970271689874 1101 28-29 1.257660331926509 1101 30-31 1.0189040252905315 1101 32-33 1.1957326907731414 1101 34-35 0.9623992573190421 1101 36-37 0.6658272180354103 1101 38-39 0.11283538798112147 1101 40-41 0.31575048228972236 1101 42-43 -0.29878641380526005 1101 44-45 0.3876306578373878 1101 46-47 0.6178944863930056 1101 48-49 0.25661213988680487 1101 50-51 0.04534058275665487 1101 52-53 0.3093391695208396 1101 54-55 0.010406184648189765 1101 56-57 0.2427408342744677 1101 58-59 -0.056458529113371014 1101 60-61 0.5847858530646555 1101 62-63 0.5367276945294464 1101 64-65 0.21832901819026063 1101 66-67 -0.4359109655893256 1101 68-69 0.14002441980553115 1101 70-71 0.3304530836096866 1101 72-73 0.8487957475850187 1101 74-75 0.7416805151937531 1101 76-77 0.4289239522911288 1101 78-79 0.6981562259554188 1101 80-81 0.4912520453257656 1101 82-83 0.3357595777470941 1101 84-85 0.5395103788010047 1101 86-87 0.11790215399298276 1101 88-89 0.1137287807159737 1101 90-91 0.07692442080460182 1101 92-93 -0.04697539609405865 1101 94-95 0.3559850486468825 1101 96-97 0.6705807865235442 1101 98-99 0.5180170641590269 1101 100-101 0.9969232736576732 1102 1 0.5619179391427274 1102 2 0.4603872301572025 1102 3 0.8859438471615739 1102 4 0.6911464652924622 1102 5 0.7005789585155 1102 6 0.6901467121109803 1102 7 0.7346279933776287 1102 8 0.7359171860921236 1102 9 0.9170325693202557 1102 10-11 0.4422452983748215 1102 12-13 0.6989196183247159 1102 14-15 1.0406393225728223 1102 16-17 1.1436143870663003 1102 18-19 0.6563242771770703 1102 20-21 0.31389103493567916 1102 22-23 0.43677158452329223 1102 24-25 0.349902129529994 1102 26-27 0.2559321017958567 1102 28-29 0.3289186238004689 1102 30-31 1.0648202773635091 1102 32-33 0.8713131219505854 1102 34-35 0.8542421263074331 1102 36-37 0.6278400704400582 1102 38-39 0.07230885398443831 1102 40-41 0.5645067011951923 1102 42-43 0.6881020604899248 1102 44-45 1.2117911055985822 1102 46-47 0.8014558462603354 1102 48-49 0.4048812111969191 1102 50-51 0.165883700501265 1102 52-53 0.007618605673410883 1102 54-55 -0.1045399181710458 1102 56-57 0.1269655440588764 1102 58-59 0.8069328473377091 1102 60-61 0.6725760686533775 1102 62-63 0.7351626033188339 1102 64-65 0.958067824160409 1102 66-67 0.7735625004139912 1102 68-69 0.13561936176241218 1102 70-71 0.4103141947208009 1102 72-73 0.1875706232069092 1102 74-75 0.1916701503512961 1102 76-77 -0.17446535119146134 1102 78-79 0.21660564552424333 1102 80-81 0.21331879491117434 1102 82-83 0.7350651333026512 1102 84-85 1.0460920537595442 1102 86-87 1.2550290196646223 1102 88-89 1.4256068901687122 1102 90-91 1.3485351173220117 1102 92-93 0.7358293302740968 1102 94-95 0.43221846489895555 1102 96-97 1.13730964224494 1102 98-99 1.4567608452535534 1102 100-101 1.2629369552497156 1103 1 -1.0366360943428106 1103 2 -1.2531782188534528 1103 3 -1.03795265207739 1103 4 -1.498922027858221 1103 5 -1.092800036918291 1103 6 -1.0150473518159586 1103 7 -0.7239907280835567 1103 8 -0.75770914571914 1103 9 -0.7684697137847678 1103 10-11 -1.0165161171502888 1103 12-13 -1.2755857089203388 1103 14-15 -1.4221175267422481 1103 16-17 -1.0072608031924446 1103 18-19 -1.2585824960344993 1103 20-21 -1.5738087367538256 1103 22-23 -1.6015655539850826 1103 24-25 -1.3393577639251006 1103 26-27 -1.0587083091630447 1103 28-29 -1.2470421828966352 1103 30-31 -1.295244410612142 1103 32-33 -1.5957721139702699 1103 34-35 -2.1067072648645606 1103 36-37 -2.2257082552829246 1103 38-39 -2.040609715269749 1103 40-41 -1.8213380476632537 1103 42-43 -1.9314280003928737 1103 44-45 -2.437609579332925 1103 46-47 -2.579917821928401 1103 48-49 -2.5083132332475238 1103 50-51 -2.622263179255203 1103 52-53 -2.6121492786492695 1103 54-55 -2.7153656411543032 1103 56-57 -2.892916495514939 1103 58-59 -2.6544446260260663 1103 60-61 -2.3435007958823917 1103 62-63 -2.401015098355444 1103 64-65 -2.229432175839591 1103 66-67 -2.6099040140456786 1103 68-69 -2.4542303338235847 1103 70-71 -2.2688619848834612 1103 72-73 -2.30229809825428 1103 74-75 -1.1839481905923854 1103 76-77 -1.748238182241689 1103 78-79 -2.0115145980069578 1103 80-81 -1.4719456647539708 1103 82-83 -0.9758842578693425 1103 84-85 -1.1622888442617665 1103 86-87 -1.1910326241709939 1103 88-89 -1.1465963061783206 1103 90-91 -0.9872240150980751 1103 92-93 -1.1512996651596907 1103 94-95 -1.2681715655424455 1103 96-97 -0.9366058828692179 1103 98-99 -0.3209218031482717 1103 100-101 -0.04713724566352795 1104 1 0.5529039582744417 1104 2 -0.16631438868017767 1104 3 -0.03532178933584973 1104 4 0.0015769288686229288 1104 5 -0.2425777743763291 1104 6 0.18780123749729682 1104 7 0.11307722075069648 1104 8 0.25086383804208623 1104 9 -0.2603956867503854 1104 10-11 0.4526619650414929 1104 12-13 0.7057260936742367 1104 14-15 0.4381972695926919 1104 16-17 0.7174738425482801 1104 18-19 0.34660665392468104 1104 20-21 0.2932646552447338 1104 22-23 0.2408094800346916 1104 24-25 0.27399613247333576 1104 26-27 0.15307614153095273 1104 28-29 0.4195413243155528 1104 30-31 0.4820848101081694 1104 32-33 0.7045544758873064 1104 34-35 0.8605657098247192 1104 36-37 0.7277526900132685 1104 38-39 1.1421672057136476 1104 40-41 1.639561888833164 1104 42-43 0.8862486756481331 1104 44-45 1.3552099797707662 1104 46-47 1.2641582009328864 1104 48-49 0.5810907402624075 1104 50-51 0.7851349882128211 1104 52-53 0.9350928514423593 1104 54-55 1.0551823040511756 1104 56-57 1.0580269862958218 1104 58-59 1.5220726560205655 1104 60-61 1.096647076747562 1104 62-63 0.7511440234807196 1104 64-65 0.2010314003193514 1104 66-67 0.36659966009022327 1104 68-69 0.4491081402760244 1104 70-71 -0.007295260761175371 1104 72-73 0.17549832740116855 1104 74-75 -0.21687933456408004 1104 76-77 -0.6310740708382561 1104 78-79 -0.020609218346251623 1104 80-81 0.1509337691569428 1104 82-83 0.06589202807822048 1104 84-85 0.12071033926358865 1104 86-87 -0.2180670068254429 1104 88-89 -0.32039200607853147 1104 90-91 -0.3986365162320702 1104 92-93 -0.06928932314753311 1104 94-95 -0.2144656778530667 1104 96-97 -0.22044422088972127 1104 98-99 -0.5524830841062691 1104 100-101 -1.1009565326090058 1105 1 0.34115770522459954 1105 2 0.1793199786952151 1105 3 0.05334150798028858 1105 4 0.2240412021345648 1105 5 0.2904912392172534 1105 6 0.033348466496946116 1105 7 0.5515139582899096 1105 8 0.2867212796593748 1105 9 0.6385237973904339 1105 10-11 0.23226722819938317 1105 12-13 -0.19325874424838219 1105 14-15 0.5291261646780825 1105 16-17 0.6034097087037296 1105 18-19 0.37470878010105224 1105 20-21 0.3539129647602337 1105 22-23 0.39473942078060276 1105 24-25 0.2049825388867177 1105 26-27 0.2115242070590213 1105 28-29 -0.16358868614104694 1105 30-31 -0.2133229974902946 1105 32-33 -0.9923564686926909 1105 34-35 -1.160195008195494 1105 36-37 -0.6746087599692956 1105 38-39 -0.4240361752553241 1105 40-41 -0.6087389128398968 1105 42-43 -0.7777239629018879 1105 44-45 -1.638537841769839 1105 46-47 -1.742769299938498 1105 48-49 -2.3800237595633114 1105 50-51 -2.372127995405169 1105 52-53 -1.8637264235663586 1105 54-55 -2.217844011738297 1105 56-57 -2.031660186935273 1105 58-59 -1.9295437608494268 1105 60-61 -1.905640305615627 1105 62-63 -2.1005464610086513 1105 64-65 -1.7064058600501184 1105 66-67 -1.8740983182994597 1105 68-69 -1.4793294686469451 1105 70-71 -1.755073232179786 1105 72-73 -1.5432407803018648 1105 74-75 -1.4850476859060109 1105 76-77 -1.3361978073318106 1105 78-79 -1.518408974358799 1105 80-81 -1.3767762343285952 1105 82-83 -1.4635825275160634 1105 84-85 -1.6953187649539103 1105 86-87 -1.06076045401959 1105 88-89 -0.858384337901466 1105 90-91 -0.9974078651341252 1105 92-93 -1.7811662837609887 1105 94-95 -1.6995432310074765 1105 96-97 -1.03155000687787 1105 98-99 -1.2252566986060938 1105 100-101 -0.03695339562747968 1106 1 0.04314917037396171 1106 2 -0.3325932503232778 1106 3 -0.3287708675531391 1106 4 -0.4541238049778116 1106 5 -0.26338500544846255 1106 6 -0.2552361707719015 1106 7 0.012218083467722352 1106 8 -0.16366990099496093 1106 9 -0.3678773405896507 1106 10-11 0.01685115423068595 1106 12-13 -0.14267197326687153 1106 14-15 0.08371364689714511 1106 16-17 0.35607684952876895 1106 18-19 -0.5001134105106146 1106 20-21 -0.5152193254246882 1106 22-23 -0.34494763469593437 1106 24-25 -0.6316106332327678 1106 26-27 -0.1993043846906275 1106 28-29 -0.18581485968301337 1106 30-31 -0.41246951242628427 1106 32-33 0.030847656485121888 1106 34-35 -0.616009375194075 1106 36-37 -0.6710525722025906 1106 38-39 -0.7754764313008451 1106 40-41 0.11743462912312452 1106 42-43 -1.3619167082788408 1106 44-45 -0.5322967322392564 1106 46-47 -0.20774085043636248 1106 48-49 0.15384892891463764 1106 50-51 -0.5411358190182547 1106 52-53 -0.6475991120443076 1106 54-55 -0.3333312094623402 1106 56-57 -0.3142882096948725 1106 58-59 -0.9040281136232515 1106 60-61 -0.32873774516043497 1106 62-63 -0.9456744837682507 1106 64-65 -1.2699727163801313 1106 66-67 -0.9694930551415659 1106 68-69 -1.254489497096447 1106 70-71 -1.0986009704443696 1106 72-73 -0.7958190164327341 1106 74-75 -0.5088741439429931 1106 76-77 0.13268554970943924 1106 78-79 -0.19968564576705106 1106 80-81 -0.4967975714551933 1106 82-83 -0.09667285468534104 1106 84-85 0.13496217387966425 1106 86-87 -0.5270655749299742 1106 88-89 -0.7945507674889463 1106 90-91 -0.1618815493446526 1106 92-93 0.12068293387770268 1106 94-95 -0.27395646002596763 1106 96-97 -0.3632534208181255 1106 98-99 0.5575491335418423 1106 100-101 0.10725001831277758 1107 1 -0.046539244155219706 1107 2 -0.5216102928435049 1107 3 -0.3164623807223599 1107 4 -0.3876751907584932 1107 5 -0.751119555284923 1107 6 -0.33360594181682757 1107 7 -0.4037743420788473 1107 8 -0.13956936734028602 1107 9 -0.07049396995787305 1107 10-11 -0.15243357635977617 1107 12-13 0.42308805427659735 1107 14-15 -0.23424125067558776 1107 16-17 0.11220957461053871 1107 18-19 -0.08887653669412288 1107 20-21 -0.029593572283012293 1107 22-23 -0.19623443104642035 1107 24-25 -0.04409557039923584 1107 26-27 -0.2398879618984111 1107 28-29 -0.26717567910115037 1107 30-31 0.06990697233872822 1107 32-33 0.3314157404927016 1107 34-35 0.4151276535544284 1107 36-37 1.2917422289680118 1107 38-39 1.0537755914225073 1107 40-41 0.4870120090508152 1107 42-43 0.7267388262365628 1107 44-45 0.6558595769361624 1107 46-47 1.4213691512851092 1107 48-49 1.2592026903193556 1107 50-51 1.19326338910707 1107 52-53 1.4381299290987464 1107 54-55 0.9723797562804748 1107 56-57 1.253602132876999 1107 58-59 1.1757403491069915 1107 60-61 1.4368630467142403 1107 62-63 1.6572477059373796 1107 64-65 1.1145168687463993 1107 66-67 1.4970674544125764 1107 68-69 1.9348718387617048 1107 70-71 1.355930790616057 1107 72-73 1.6883004533555273 1107 74-75 1.301786038532473 1107 76-77 1.6041307209953786 1107 78-79 1.7342100333515624 1107 80-81 0.7734081438142155 1107 82-83 1.2035939726515537 1107 84-85 1.6320571988409291 1107 86-87 1.6734207394098455 1107 88-89 2.1340729198926986 1107 90-91 1.4129592149865573 1107 92-93 0.9387884597857763 1107 94-95 1.955020128027055 1107 96-97 1.8195017866186127 1107 98-99 1.854717320837418 1107 100-101 2.0192400338059766 1108 1 0.7355290502538416 1108 2 0.7381650079349811 1108 3 0.8720549582726882 1108 4 0.3092020208480193 1108 5 0.9366900696266143 1108 6 1.0165356009998732 1108 7 0.9846279933776287 1108 8 0.7498060749810165 1108 9 1.15314368043137 1108 10-11 1.265161965041493 1108 12-13 1.011419618324716 1108 14-15 1.3080004336839366 1108 16-17 1.313753275955186 1108 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-0.6577042195094513 2316 94-95 -1.2319680430375506 2316 96-97 -1.6319003144650193 2316 98-99 -1.6842023582096495 2316 100-101 -2.4309212698585103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3187.0 3 519.0 4 394.0 5 327.0 6 369.0 7 408.0 8 504.0 9 554.0 10 731.0 11 1070.0 12 1585.0 13 2104.0 14 2571.0 15 3321.0 16 4119.0 17 5127.0 18 3868.0 19 2483.0 20 2368.0 21 2342.0 22 2313.0 23 2253.0 24 2346.0 25 2530.0 26 2872.0 27 3162.0 28 3392.0 29 4015.0 30 4535.0 31 5316.0 32 6207.0 33 7218.0 34 9308.0 35 11387.0 36 14779.0 37 18483.0 38 7727.0 39 262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00934643431819 24.158307371015784 16.199116710602894 16.63322948406313 2 16.507209825100063 23.706069411700202 40.48399035034815 19.30273041285158 3 18.09613895114913 28.399580514486644 37.296511827161 16.207768707203225 4 11.092147831331909 23.020507758951585 40.10390963426002 25.783434775456488 5 8.6899275839368 42.00817423743691 36.434331797235025 12.867566381391265 6 21.584375535377763 43.57649477471304 19.27085831762892 15.568271372280282 7 20.465938039280136 43.556715150519075 20.332402005094913 15.64494480510587 8 25.342235918507107 34.41533983906865 20.647149460708782 19.59527478171546 9 26.76244410509953 17.27623208452884 24.446529209151358 31.51479460122027 10-11 22.388632214889224 29.97092296469315 28.44309428973605 19.197350530681582 12-13 24.080524132923724 31.826416929520835 28.239613407446225 15.853445530109218 14-15 21.745412710532534 30.556706396354137 30.873425392988356 16.824455500124976 16-17 15.673826620234829 34.58726685636606 31.111042627576264 18.627863895822845 18-19 15.165513250305619 30.924456650150155 38.73218939085234 15.177840708691887 20-21 16.745957535715142 28.992029811996726 39.30737798967677 14.954634662611358 22-23 16.998757015330142 29.441273254599558 38.50718568410383 15.052784045966463 24-25 15.975437683224197 31.330652894599854 38.9291487438012 13.76476067837475 26-27 15.544458487031157 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17.6635523617478 52-53 16.883610418777902 32.42721371641423 33.95791679219961 16.731259072608253 54-55 16.81622256382516 31.479661881562688 33.625597852694575 18.07851770191758 56-57 17.178737477831568 32.710334769003225 33.286200450558404 16.824727302606803 58-59 17.337551399117356 31.35474549519476 33.07518222934363 18.232520876344253 60-61 16.302807015141035 30.7347271395016 34.37138337430749 18.591082471049873 62-63 16.579991577851803 30.45291300194805 34.14530602183596 18.821789398364185 64-65 16.302055516754997 30.465743669802663 34.193336346082745 19.0388644673596 66-67 16.722642756146243 31.629400627890814 32.77186063151984 18.876095984443097 68-69 16.396832515671377 29.829540591523934 33.78786893215517 19.98575796064952 70-71 16.392224539200882 30.849828965885628 34.29893133639448 18.459015158519005 72-73 17.537065570963875 29.409005307310395 34.42698168121897 18.626947440506765 74-75 16.250179766201214 30.586277504228786 34.042582332920155 19.120960396649842 76-77 16.645894679175512 30.504006026159008 33.74443607477915 19.105663219886324 78-79 16.16814847021044 30.58038541727291 33.133287002009595 20.118179110507054 80-81 16.45623788950435 29.15625363742802 33.70911530903589 20.678393164031743 82-83 16.542792291601764 30.287541467389257 32.9524096447413 20.217256596267678 84-85 16.413444618660606 29.92584782028196 33.03126989887094 20.629437662186497 86-87 16.773314434595648 30.664571371836086 32.03472985418529 20.527384339382984 88-89 17.518885563416458 30.670634404257214 31.442494058666814 20.36798597365952 90-91 17.662487972414453 29.618507243945118 32.04902118567168 20.66998359796875 92-93 18.260667093544445 29.041271319634436 32.3640696746712 20.333991912149923 94-95 18.492972410203016 29.292925833584484 31.728609002986385 20.485492753226115 96-97 19.79788432627538 28.713301896161447 30.28961237761591 21.199201399947263 98-99 19.49798158603853 28.726190761519 29.36749514656185 22.408332505880622 100-101 20.681764462059682 28.67165206786814 30.346774276425997 20.299809193646183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 766.0 1 455.0 2 176.5 3 192.5 4 132.0 5 111.5 6 202.5 7 359.0 8 496.5 9 546.5 10 487.5 11 388.5 12 360.0 13 377.0 14 436.5 15 538.0 16 603.5 17 676.5 18 786.5 19 877.5 20 1007.5 21 1143.5 22 1267.5 23 1425.5 24 1602.5 25 2021.5 26 3003.5 27 4210.5 28 4498.0 29 4696.5 30 5624.5 31 6332.0 32 6444.5 33 6458.0 34 7010.5 35 7648.0 36 7877.0 37 7692.0 38 6160.5 39 4736.0 40 4337.5 41 4156.5 42 4184.5 43 4323.0 44 4508.5 45 3867.0 46 2847.5 47 2249.5 48 2261.5 49 2251.5 50 1976.5 51 1678.5 52 1332.0 53 1027.5 54 802.5 55 664.0 56 534.5 57 441.0 58 394.5 59 356.5 60 311.0 61 281.5 62 245.0 63 206.5 64 178.0 65 161.5 66 146.0 67 141.5 68 120.0 69 94.5 70 79.5 71 72.5 72 68.5 73 56.5 74 51.5 75 44.5 76 36.0 77 30.0 78 25.0 79 20.5 80 16.0 81 10.5 82 5.5 83 2.0 84 2.0 85 3.0 86 1.5 87 0.5 88 2.5 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007531357835350825 2 0.09859232075368353 3 0.11160102974201677 4 0.10954702305964836 5 0.15884318343649012 6 0.08969162513008709 7 0.019170729035438462 8 0.021224735717806868 9 0.01574738456482445 10-11 0.04416114367092074 12-13 0.04279180588267514 14-15 0.01882839458837706 16-17 0.02430574574135948 18-19 0.02772909021197349 20-21 0.051007832612148765 22-23 0.02499041463548228 24-25 0.041080133647368136 26-27 0.048953825929780356 28-29 0.044845812565043545 30-31 0.060935531576929394 32-33 0.041080133647368136 34-35 0.05443117708276277 36-37 0.044845812565043545 38-39 0.022936407953113875 40-41 0.0301254313414033 42-43 0.028756093553157693 44-45 0.007873692282412225 46-47 0.010612367858903434 48-49 0.006162020047105221 50-51 0.006504354494166621 52-53 0.008216026729473627 54-55 0.008558361176535028 56-57 0.010270033411842034 58-59 0.011981705647149038 60-61 0.019855397929561265 62-63 0.008558361176535028 64-65 0.006846688941228023 66-67 0.007873692282412225 68-69 0.006504354494166621 70-71 0.022251739058991072 72-73 0.021224735717806868 74-75 0.022594073506052473 76-77 0.01780139124719286 78-79 0.004450347811798215 80-81 0.0034233444706140113 82-83 0.005135016705921017 84-85 0.0034233444706140113 86-87 0.009927698964780633 88-89 0.0308101002355261 90-91 0.025675083529605083 92-93 0.023621076847236674 94-95 0.041080133647368136 96-97 0.03457577915320151 98-99 0.017459056800131458 100-101 0.06607054828285042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 146056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.39913457851783 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6519644918594 28.431560497343483 2 7.205127184427372 4.957002793449088 3 2.5377174475538395 2.6188585200197187 4 1.4848135026471876 2.043051980062442 5 0.9076071812427848 1.561045078599989 6 0.6030810875363242 1.2447280495152544 7 0.44783249074479514 1.0783535082434135 8 0.4020540583575495 1.1064249329024483 9 0.31447792683412285 0.9735991674426246 >10 2.6889853110943034 19.40488579722846 >50 0.45579395724692484 11.084104726954044 >100 0.28462242745113653 18.47442077011557 >500 0.007961466502129692 1.6548447170948128 >1k 0.007961466502129692 5.367119461028647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 1.747959686695514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2445 1.6740154461302514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 1.1386043709262201 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1178 0.806539957276661 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 643 0.44024209892096183 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 636 0.4354494166621022 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 580 0.3971079585912253 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 558 0.3820452429205236 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 496 0.3395957714849099 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 479 0.32795640028482226 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 467 0.3197403735553486 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 443 0.3033083200964014 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 436 0.29851563783754176 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 429 0.29372295557868217 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 429 0.29372295557868217 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 390 0.2670208687078929 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 380 0.26017417976666485 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 379 0.259489510872542 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 364 0.24921947746070003 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 358 0.2451114640959632 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.2410034507312264 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 344 0.23552609957824397 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 335 0.22936407953113874 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 329 0.22525606616640192 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 304 0.20813934381333188 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 297 0.20334666155447226 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 296 0.20266199266034943 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 296 0.20266199266034943 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 296 0.20266199266034943 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 294 0.20129265487210385 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 291 0.19923864818973547 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 275 0.1882839458837706 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 274 0.18759927698964782 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 273 0.18691460809552501 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 267 0.1828065947307882 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 252 0.17253656131894615 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 247 0.16911321684833214 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 243 0.16637454127184095 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 238 0.1629511968012269 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 235 0.16089719011885853 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 235 0.16089719011885853 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.1602125212247357 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 224 0.1533658322835077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 224 0.1533658322835077 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 218 0.14925781891877088 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 218 0.14925781891877088 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 215 0.14720381223640247 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 214 0.14651914334227967 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 214 0.14651914334227967 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 211 0.1444651366599113 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 202 0.13830311661280603 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 200 0.13693377882456045 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 199 0.13624910993043762 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 198 0.13556444103631485 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 189 0.12940242098920962 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 183 0.1252944076244728 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 181 0.1239250698362272 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 179 0.1225557320479816 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 179 0.1225557320479816 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 178 0.12187106315385879 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 178 0.12187106315385879 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 172 0.11776304978912198 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 171 0.11707838089499917 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 168 0.11502437421263077 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 166 0.11365503642438517 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 166 0.11365503642438517 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 165 0.11297036753026236 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 163 0.11160102974201677 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 162 0.11091636084789395 No Hit GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA 162 0.11091636084789395 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 160 0.10954702305964836 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 159 0.10886235416552555 No Hit GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG 158 0.10817768527140274 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 157 0.10749301637727995 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 155 0.10612367858903433 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 155 0.10612367858903433 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 154 0.10543900969491155 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 153 0.10475434080078874 No Hit ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT 153 0.10475434080078874 No Hit GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA 153 0.10475434080078874 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 152 0.10406967190666594 No Hit CCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT 151 0.10338500301254315 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 149 0.10201566522429753 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 149 0.10201566522429753 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 147 0.10064632743605192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.423344470614011E-4 0.0 20-21 0.0 0.0 0.0 0.002738675576491209 0.0 22-23 0.0 0.0 0.0 0.014378046776578847 0.0 24-25 0.0 0.0 0.0 0.0311524346825875 0.0 26-27 0.0 0.0 0.0 0.06401654160048201 0.0 28-29 0.0 0.0 0.0 0.13556444103631485 0.0 30-31 0.0 0.0 0.0 0.29474995891986633 0.0 32-33 0.0 0.0 0.0 0.5022046338390754 0.0 34-35 0.0 0.0 0.0 0.8335843785945116 0.0 36-37 0.0 0.0 0.0 1.2553404173741578 0.0 38-39 0.0 0.0 0.0 1.7407706633072246 0.0 40-41 0.0 0.0 0.0 2.2049761735224847 0.0 42-43 0.0 0.0 0.0 2.6393985868434027 0.0 44-45 0.0 0.0 0.0 3.1207208194117326 0.0 46-47 0.0 0.0 0.0 3.651339212356904 0.0 48-49 0.0 0.0 0.0 4.175110916360848 0.0 50-51 0.0 0.0 0.0 4.699567289258915 0.0 52-53 0.0 0.0 0.0 5.152818097168209 0.0 54-55 0.0 0.0 0.0 5.6632387577367584 0.0 56-57 0.0 0.0 0.0 6.281494769129649 0.0 58-59 0.0 0.0 0.0 6.8696253491811365 0.0 60-61 0.0 0.0 0.0 7.501574738456482 0.0 62-63 0.0 0.0 0.0 8.091759325190338 0.0 64-65 0.0 0.0 0.0 8.638125102700334 0.0 66-67 0.0 0.0 0.0 9.291641562140548 0.0 68-69 0.0 0.0 0.0 10.016021252122474 0.0 70-71 0.0 0.0 0.0 10.825642219422686 0.0 72-73 0.0 0.0 0.0 11.594525387522594 0.0 74-75 0.0 0.0 0.0 12.346291833269431 0.0 76-77 0.0 0.0 0.0 13.232595716711398 0.0 78-79 0.0 0.0 0.0 14.082612148764857 0.0 80-81 0.0 0.0 0.0 15.033617242701428 0.0 82-83 0.0 0.0 0.0 15.844265213342828 0.0 84-85 0.0 0.0 0.0 16.650120501725368 0.0 86-87 0.0 0.0 0.0 17.506298953825933 0.0 88-89 0.0 0.0 0.0 18.372405104891275 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCAA 15 6.602091E-4 95.01507 9 GACGTAT 15 6.602091E-4 95.01507 6 ATGGGTT 20 0.0020635168 71.310165 5 GGTACCA 20 0.002069137 71.26131 8 CTGGGCT 45 1.5018701E-5 52.822342 4 GGTATCA 1505 0.0 51.17274 1 TGGGTAC 40 5.368438E-4 47.507538 6 AGGTTAG 45 9.557631E-4 42.257874 1 GAGTACC 50 0.001604526 38.03209 1 AGTGTTT 25 0.0016125839 38.00603 74-75 GTATCAA 2195 0.0 35.08655 2 GTTAGAG 55 0.0025616523 34.574627 3 GGTTAGA 55 0.0025616523 34.574627 2 GGGTGAG 100 1.2873206E-6 33.255276 8 GGTGAGA 100 1.2873206E-6 33.255276 9 CTAGATG 75 3.050245E-4 31.693405 4 ATGGGAT 110 2.6941452E-6 30.2528 5 ATGGGGT 175 1.4733814E-10 29.882355 5 GTGGTAT 670 0.0 29.801262 1 CACAAGA 40 4.4813557E-4 29.692211 92-93 >>END_MODULE