FastQCFastQC Report
Thu 26 May 2016
SRR938363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146056
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24941.7075642219422686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24581.6829161417538478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17631.2070712603385003No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14440.9886618831133265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13390.9167716492304322No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT9470.6483814427342938TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.45188147012104946No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6450.44161143670920744No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT6020.41217067426192694RNA PCR Primer, Index 1 (95% over 23bp)
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5990.4101166675795585No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5570.38136057402640083No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5430.3717752095086816No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA5040.3450731226378923No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4820.33001040696719064No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4770.32658706249657665No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4430.3033083200964014No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4420.3026236512022786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.28824560442569974No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA4030.2759215643314893No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3750.25675083529605086No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3660.2505888152489456No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA3390.23210275510762995No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3340.2286794106370159No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA3320.22731007284877033No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA3310.2266254039546475No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT3230.22114805280166513No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT3200.21909404611929673No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT3160.2163553705428055No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT3090.2115626882839459No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.20540066823684067No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT2890.19786931040148983No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT2870.19649997261324423No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2820.1930766281426302No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC2780.19033795256613903No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2760.1889686147778934No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2720.18622993920140218No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.1855452703072794No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT2670.1828065947307882No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC2620.17938325026017418No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG2570.17595990578956017No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2470.16911321684833214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2470.16911321684833214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2400.16432053458947254No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT2390.16363586569534974No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA2340.1602125212247357No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA2340.1602125212247357No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG2330.15952785233061292No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA2330.15952785233061292No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT2240.1533658322835077TruSeq Adapter, Index 1 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.14925781891877088No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA2180.14925781891877088No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC2180.14925781891877088No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC2160.14788848113052527No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2150.14720381223640247No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC2140.14651914334227967No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2110.1444651366599113No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT2110.1444651366599113No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA2070.14172646108342007No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2070.14172646108342007No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT2000.13693377882456045No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA1930.13214109656570083No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1900.13008708988333242No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA1890.12940242098920962No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1870.12803308320096402No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG1830.1252944076244728No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1239250698362272No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA1790.1225557320479816No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT1790.1225557320479816No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC1770.12118639425973599No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA1770.12118639425973599No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC1740.11913238757736758No Hit
AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT1710.11707838089499917No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC1700.11639371200087639No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA1700.11639371200087639No Hit
GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT1690.11570904310675358No Hit
GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC1680.11502437421263077No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG1640.11228569863613955TruSeq Adapter, Index 1 (95% over 24bp)
GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT1600.10954702305964836No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA1590.10886235416552555No Hit
CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT1580.10817768527140274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1530.10475434080078874No Hit
AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA1530.10475434080078874No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC1520.10406967190666594No Hit
CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA1510.10338500301254315No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG1500.10270033411842033No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC1500.10270033411842033No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT1500.10270033411842033No Hit
AGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGA1490.10201566522429753No Hit
AATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT1480.10133099633017471No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC1480.10133099633017471No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG1480.10133099633017471No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT1480.10133099633017471No Hit
GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA1470.10064632743605192No Hit
CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA1470.10064632743605192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.041.2245671
CCATAGG1054.6233254E-836.1787381
CATAGGG1106.9612724E-834.534252
AGGGTCT1255.5988494E-934.188915
ATAGGGT1151.0288204E-733.032763
TAGGGTC1151.0288204E-733.032764
GTATCAA23550.032.059661
GTTGTGG300.00394582131.66723864-65
GTAATGC804.483178E-429.677874
TGTAATG804.483178E-429.677873
TAATGCT804.483178E-429.677875
AATGCTA856.3923415E-427.9321146
CAACCAC350.00836676927.15264582-83
TATCAAC28750.026.5913732
GGTCTTC1501.0525146E-625.3251177
TCAACGC30300.025.2310894
AACGCAG31150.024.5425996
GTAAGGT1551.4005564E-624.5081774
CAACGCA31250.024.4640645
ATCAACG31900.023.9655783
Produced by FastQC (version 0.11.5)