##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938363_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146056 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.658973270526374 31.0 31.0 34.0 30.0 34.0 2 31.890206496138468 31.0 31.0 34.0 30.0 34.0 3 31.840027112888208 31.0 31.0 34.0 30.0 34.0 4 35.6003724598784 37.0 35.0 37.0 33.0 37.0 5 35.53823875773676 37.0 35.0 37.0 33.0 37.0 6 35.58087993646273 37.0 35.0 37.0 33.0 37.0 7 35.523614230158294 37.0 35.0 37.0 33.0 37.0 8 35.54139508133866 37.0 35.0 37.0 33.0 37.0 9 36.98844963575615 39.0 37.0 39.0 33.0 39.0 10-11 36.954972750178015 39.0 37.0 39.0 33.0 39.0 12-13 36.8606356466013 39.0 37.0 39.0 32.0 39.0 14-15 37.714335597305144 39.5 36.5 41.0 32.0 41.0 16-17 37.65404707783316 39.0 36.5 41.0 32.0 41.0 18-19 38.02456591992113 40.0 37.0 41.0 33.0 41.0 20-21 38.13422933669277 40.0 37.0 41.0 33.0 41.0 22-23 37.99241729199759 40.0 37.0 41.0 33.0 41.0 24-25 37.81016870241551 40.0 37.0 41.0 33.0 41.0 26-27 37.554150462836176 39.5 36.0 41.0 32.0 41.0 28-29 37.340677548337624 39.0 36.0 41.0 32.0 41.0 30-31 36.843642164649175 39.0 35.0 40.5 31.0 41.0 32-33 36.35575395738621 38.0 35.0 40.0 30.5 41.0 34-35 35.90158911650326 38.0 35.0 40.0 30.0 41.0 36-37 35.648781974037355 38.0 35.0 40.0 30.0 41.0 38-39 35.24405022731007 38.0 35.0 40.0 28.0 41.0 40-41 34.95576011940626 38.0 35.0 40.0 26.5 41.0 42-43 34.4396430136386 38.0 34.0 40.0 24.0 41.0 44-45 33.65362942980775 38.0 33.0 40.0 16.0 41.0 46-47 32.62036821493126 38.0 33.0 40.0 2.0 41.0 48-49 31.77834186887221 38.0 31.5 40.0 2.0 41.0 50-51 31.16591923645725 37.0 31.0 40.0 2.0 41.0 52-53 30.65013761844772 37.0 30.0 40.0 2.0 41.0 54-55 30.160198827846855 36.5 28.5 40.0 2.0 41.0 56-57 29.693107438242865 36.0 27.5 40.0 2.0 41.0 58-59 29.050857205455443 36.0 26.0 39.0 2.0 41.0 60-61 28.532966807252013 35.0 24.0 39.0 2.0 40.0 62-63 27.907463575614834 35.0 21.5 39.0 2.0 40.0 64-65 27.216338253820453 34.5 16.0 38.0 2.0 40.0 66-67 26.5507921619105 34.0 7.5 38.0 2.0 40.0 68-69 25.95204236731117 34.0 2.0 37.0 2.0 39.0 70-71 25.48701183107849 33.0 2.0 36.5 2.0 39.0 72-73 25.042470011502438 33.0 2.0 36.0 2.0 39.0 74-75 24.522857671030287 32.5 2.0 35.5 2.0 38.0 76-77 23.39729281919264 30.5 2.0 34.0 2.0 36.0 78-79 23.931351673330777 32.0 2.0 35.0 2.0 37.0 80-81 23.597486580489672 32.0 2.0 35.0 2.0 36.0 82-83 23.224838418140987 31.0 2.0 35.0 2.0 36.0 84-85 22.869063920687957 31.0 2.0 35.0 2.0 35.5 86-87 22.41915429698198 31.0 2.0 34.0 2.0 35.0 88-89 21.84315262638988 30.0 2.0 34.0 2.0 35.0 90-91 21.414159637399354 29.5 2.0 34.0 2.0 35.0 92-93 20.853874541271843 29.0 2.0 34.0 2.0 35.0 94-95 20.238093607931205 29.0 2.0 34.0 2.0 35.0 96-97 19.459385441200638 27.5 2.0 34.0 2.0 35.0 98-99 18.448437585583612 25.5 2.0 34.0 2.0 35.0 100-101 15.354983704880318 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1964083503696088 1101 2 0.5296751083697409 1101 3 0.2097917325792693 1101 4 0.1721157477619002 1101 5 0.30920665500072886 1101 6 0.4359405064606676 1101 7 0.23727103774237435 1101 8 0.18311328117691517 1101 9 0.5214591637447654 1101 10-11 0.3249674192198242 1101 12-13 0.28935509905203105 1101 14-15 0.26221466840562613 1101 16-17 0.026694242867627338 1101 18-19 0.40872821789502467 1101 20-21 0.42500476389425046 1101 22-23 0.025939269597188286 1101 24-25 0.31219932564364683 1101 26-27 -0.053688974641829645 1101 28-29 0.034835435094500156 1101 30-31 0.3564928270733745 1101 32-33 0.5142841223562655 1101 34-35 -0.07294328338871026 1101 36-37 0.4073962035342049 1101 38-39 0.8041441093091706 1101 40-41 0.6079834003589468 1101 42-43 -0.0665172391806621 1101 44-45 -0.5096208864749698 1101 46-47 -0.5811777183663196 1101 48-49 -1.837412745569516 1101 50-51 -1.348478322083917 1101 52-53 -1.7159059928538483 1101 54-55 -1.856051026014235 1101 56-57 -2.059709536301508 1101 58-59 -1.4952289353191155 1101 60-61 -1.6286771622928597 1101 62-63 -1.0740841309328815 1101 64-65 -0.7890940880783397 1101 66-67 -1.4076823406618573 1101 68-69 -1.4644177143404526 1101 70-71 -0.7323955876783401 1101 72-73 -1.324302335809108 1101 74-75 -1.2706627007020046 1101 76-77 -0.8884139972144389 1101 78-79 -0.6968702181954427 1101 80-81 -0.7168688898162117 1101 82-83 -0.47080083827481545 1101 84-85 -0.7744322750655854 1101 86-87 -0.9105073631659266 1101 88-89 -0.946967530045157 1101 90-91 -0.887537963625455 1101 92-93 -1.0047454662657778 1101 94-95 -0.1591699753190845 1101 96-97 -0.19321487420731387 1101 98-99 -0.8019881695761164 1101 100-101 0.6307420006238207 1102 1 -0.008504584953776373 1102 2 0.2341734499949446 1102 3 0.20025607752122454 1102 4 0.20694161840867054 1102 5 0.263497699776849 1102 6 0.40816272868288905 1102 7 0.2127063611254556 1102 8 0.22415805729632154 1102 9 -0.17837499877596485 1102 10-11 0.1298492600158383 1102 12-13 0.5570271554367707 1102 14-15 0.4130749503293387 1102 16-17 0.25886671384606785 1102 18-19 0.3394392460873945 1102 20-21 0.07337748362891006 1102 22-23 0.08341232100680429 1102 24-25 0.2820376341013642 1102 26-27 -0.2247606993516129 1102 28-29 0.1585916539999772 1102 30-31 -0.015294071765758588 1102 32-33 -0.036607254094811026 1102 34-35 0.4023883915698292 1102 36-37 0.42900690005162545 1102 38-39 0.4304397146159644 1102 40-41 0.5606678945546335 1102 42-43 0.2623073876850128 1102 44-45 0.6201469410540525 1102 46-47 1.117827256758062 1102 48-49 1.0277924783110812 1102 50-51 1.2010656248480949 1102 52-53 0.8958270088045239 1102 54-55 1.082900051929382 1102 56-57 0.9389948583916947 1102 58-59 1.2239356583790588 1102 60-61 0.038352273859711516 1102 62-63 0.12321064518652136 1102 64-65 0.3458043364656085 1102 66-67 0.44741716182570457 1102 68-69 0.3807315393908901 1102 70-71 0.1883651917578142 1102 72-73 0.847287631023395 1102 74-75 0.9203095215202168 1102 76-77 0.854123316218395 1102 78-79 1.097750428570727 1102 80-81 1.27483923622027 1102 82-83 1.2856253640469042 1102 84-85 0.6942659007221401 1102 86-87 -0.04981084575299022 1102 88-89 0.040957428495474346 1102 90-91 0.1282684211174967 1102 92-93 0.3393154790078512 1102 94-95 0.30735158355322056 1102 96-97 0.3822930030729488 1102 98-99 0.9739757607721451 1102 100-101 1.2714965611545015 1103 1 0.2086757803430288 1103 2 -0.15842548455604444 1103 3 0.41220432714070654 1103 4 -0.003104043691791958 1103 5 -0.220235176935482 1103 6 -0.2010457949091986 1103 7 0.08627942048618564 1103 8 0.14539093400865255 1103 9 0.2424773604325594 1103 10-11 0.44677277599758014 1103 12-13 0.25684640962246874 1103 14-15 0.4113150568742441 1103 16-17 0.15079974276540042 1103 18-19 0.3445286677007928 1103 20-21 0.2588322020459586 1103 22-23 0.23300326469021115 1103 24-25 -0.013909854483109996 1103 26-27 -0.1308204405997131 1103 28-29 -0.10567775239272237 1103 30-31 -0.20888235182663806 1103 32-33 0.09172227406348554 1103 34-35 0.6190931099868777 1103 36-37 0.35352174023426386 1103 38-39 -0.01624027016332974 1103 40-41 0.5821195688316507 1103 42-43 0.2849957438493931 1103 44-45 0.08780295323060727 1103 46-47 -0.2737252546574638 1103 48-49 0.2037342591329967 1103 50-51 -0.01449754105754053 1103 52-53 -1.0891425497951737 1103 54-55 -0.8125527639001469 1103 56-57 -0.33864022532215543 1103 58-59 -0.5419985120927784 1103 60-61 -0.6220834186821484 1103 62-63 -0.4063270260463554 1103 64-65 -0.17326910341262902 1103 66-67 -0.1147974500464386 1103 68-69 -0.10043284417075427 1103 70-71 -0.4686458432497993 1103 72-73 -0.8321739367087204 1103 74-75 -1.1884709198800891 1103 76-77 -0.7760136700829747 1103 78-79 -0.4337849595571299 1103 80-81 -0.8054499570826223 1103 82-83 -0.2522361275817069 1103 84-85 -0.17654840673599992 1103 86-87 -0.5418199781730806 1103 88-89 0.010658722254984099 1103 90-91 0.6208958944812721 1103 92-93 0.9490472141676669 1103 94-95 0.310110335455807 1103 96-97 0.30642732575179465 1103 98-99 1.141070509630591 1103 100-101 1.4973336996628728 1104 1 0.2529468499248111 1104 2 0.1671664595608 1104 3 0.32479618053815074 1104 4 0.22129040428063007 1104 5 -0.3768168697595726 1104 6 0.20415242699046843 1104 7 0.10605625075017855 1104 8 0.3085951433890344 1104 9 0.20941013735354375 1104 10-11 0.21725268164939138 1104 12-13 0.2100578765552399 1104 14-15 0.11241729764354602 1104 16-17 0.47262683157969576 1104 18-19 0.2854703351234562 1104 20-21 0.20594278900050966 1104 22-23 0.6989154483062805 1104 24-25 0.2871424906134976 1104 26-27 0.3530768650339624 1104 28-29 0.48116339792933616 1104 30-31 0.4272647950480746 1104 32-33 0.16091850013624054 1104 34-35 0.22017720687519926 1104 36-37 0.668590233384954 1104 38-39 0.9237942032105266 1104 40-41 0.8004811763795061 1104 42-43 0.18345804993666803 1104 44-45 -0.34361317667957536 1104 46-47 -0.0731219706150128 1104 48-49 0.5851186372514796 1104 50-51 0.469921401154199 1104 52-53 0.3423869793711134 1104 54-55 0.585705423526143 1104 56-57 -0.3482338023882896 1104 58-59 0.29087366426574235 1104 60-61 -0.5178241426229988 1104 62-63 -0.8641651826280459 1104 64-65 -0.2965567746455058 1104 66-67 0.07131340906632389 1104 68-69 0.4560626652187061 1104 70-71 0.21165897395060185 1104 72-73 0.023083252804116938 1104 74-75 0.5356286900264706 1104 76-77 0.3193551042978662 1104 78-79 0.4661968965618968 1104 80-81 0.7018075953078338 1104 82-83 0.87107422350239 1104 84-85 0.7738280787942564 1104 86-87 0.5699463286399435 1104 88-89 0.6155757139995188 1104 90-91 0.7755044402492146 1104 92-93 0.5301957218334614 1104 94-95 0.38125702873350065 1104 96-97 0.3192641215424139 1104 98-99 0.0593096459818554 1104 100-101 0.6483361490868038 1105 1 -0.11486423407658108 1105 2 0.2455038593516683 1105 3 0.14616251026976101 1105 4 -0.040865399135192604 1105 5 0.16152401556632157 1105 6 -0.06405949353933238 1105 7 0.13485548393247626 1105 8 0.08600016255947907 1105 9 -0.04825803971164078 1105 10-11 0.10079224247198226 1105 12-13 0.022122184676533152 1105 14-15 0.36683957021237745 1105 16-17 0.07721466706244229 1105 18-19 0.10241439228622795 1105 20-21 0.28920350702072284 1105 22-23 0.25355120989569 1105 24-25 0.21405517796100781 1105 26-27 -0.12370075783115198 1105 28-29 -0.20873290740353667 1105 30-31 -0.3084227267365165 1105 32-33 -0.13894643538135654 1105 34-35 -0.913035585038358 1105 36-37 -0.6636466087203061 1105 38-39 -0.762725490062401 1105 40-41 -0.52467567269683 1105 42-43 -0.5715741912623571 1105 44-45 -0.5468852226886369 1105 46-47 -0.17274291868053382 1105 48-49 -0.33898383747839134 1105 50-51 -0.08767706521038932 1105 52-53 -0.18458234792062456 1105 54-55 -0.5548923457314388 1105 56-57 -0.802050463245731 1105 58-59 -0.9886008913285416 1105 60-61 -1.1372690711695306 1105 62-63 -1.4573814600766362 1105 64-65 -1.0103725641191907 1105 66-67 -0.6468810837883296 1105 68-69 -1.0370316185038462 1105 70-71 -1.1977824690609005 1105 72-73 -1.6284068134210479 1105 74-75 -1.5996100691230595 1105 76-77 -1.10969247325529 1105 78-79 -0.7174907994994513 1105 80-81 -0.6750876643645256 1105 82-83 -0.6822108932630329 1105 84-85 -0.6251785437223027 1105 86-87 -0.13753014399860675 1105 88-89 -0.1280849691653465 1105 90-91 -0.42308391806378864 1105 92-93 -1.114083643799166 1105 94-95 -0.6063911065052601 1105 96-97 -0.7118224940030728 1105 98-99 -0.9892917830875056 1105 100-101 0.5339234617392972 1106 1 0.08214856819937921 1106 2 0.0947214980429969 1106 3 0.1589647862299337 1106 4 0.20468936615641553 1106 5 0.2077544565336069 1106 6 0.19945401997418344 1106 7 0.07813428905338782 1106 8 0.09577967891794259 1106 9 -0.46290953331050133 1106 10-11 0.41729295370953423 1106 12-13 0.3228867650463769 1106 14-15 0.18325137675576286 1106 16-17 0.2990318790112312 1106 18-19 0.45421026710841517 1106 20-21 0.38184219834362665 1106 22-23 0.16233499367947246 1106 24-25 0.5157088052725314 1106 26-27 0.7342295408886272 1106 28-29 0.8094925549008707 1106 30-31 0.4676263486546617 1106 32-33 0.37198884200128646 1106 34-35 0.6779139170953528 1106 36-37 0.543590233384954 1106 38-39 0.06266569059194183 1106 40-41 0.2752887654255005 1106 42-43 0.4118006309282549 1106 44-45 -0.21731552140841615 1106 46-47 -1.1513168873860842 1106 48-49 -1.2872413054727012 1106 50-51 -0.7613968376143703 1106 52-53 -1.169112931135416 1106 54-55 -1.4439392874099575 1106 56-57 -1.3114743608275248 1106 58-59 -1.0169614887680858 1106 60-61 -1.328858687101249 1106 62-63 -1.0972285440026681 1106 64-65 -0.9012865043752356 1106 66-67 -1.2293846399760966 1106 68-69 -1.7104846768253275 1106 70-71 -1.4693863097436868 1106 72-73 -0.9088122188264514 1106 74-75 -1.0409329709722748 1106 76-77 -0.770876683781605 1106 78-79 -0.6941977696274719 1106 80-81 -1.4443799829989459 1106 82-83 -1.1843446059230622 1106 84-85 -1.5035569221006817 1106 86-87 -1.0441802151223598 1106 88-89 -0.4850373162492687 1106 90-91 -0.6801962935972163 1106 92-93 -0.7212138002714248 1106 94-95 -0.8290973653957288 1106 96-97 -1.3045500900201432 1106 98-99 -1.804458923912545 1106 100-101 -1.2255004358424966 1107 1 0.32018437470652117 1107 2 0.24642575006571477 1107 3 0.2807585545205171 1107 4 0.04239809399253147 1107 5 -0.05218701359894595 1107 6 -0.15641618143742164 1107 7 -0.019645018917003654 1107 8 0.1047313057039645 1107 9 0.41327397503861363 1107 10-11 0.12036146808377879 1107 12-13 0.10561615048277417 1107 14-15 0.21135432046380487 1107 16-17 0.27762113705909286 1107 18-19 0.25396065443842275 1107 20-21 0.3838649217039247 1107 22-23 0.2806212735899578 1107 24-25 0.31481364259392564 1107 26-27 -0.031134549316227833 1107 28-29 0.04367657968999339 1107 30-31 0.26836657933120023 1107 32-33 0.2916393842632985 1107 34-35 0.5801130907913432 1107 36-37 0.37878131618749933 1107 38-39 0.37534859642772034 1107 40-41 0.5103980785602928 1107 42-43 1.3357548399143155 1107 44-45 1.4947046197518574 1107 46-47 1.8545576176922438 1107 48-49 0.9591220961454745 1107 50-51 1.036610564692392 1107 52-53 0.5742594221095487 1107 54-55 0.5428699740808298 1107 56-57 1.3308694160703958 1107 58-59 0.9413144623422589 1107 60-61 1.3743174189410965 1107 62-63 1.2281071420018073 1107 64-65 1.10790182408061 1107 66-67 0.9789989028023527 1107 68-69 1.4515914120023545 1107 70-71 1.540253903718181 1107 72-73 1.378758791674496 1107 74-75 1.0844328406992716 1107 76-77 0.718772997747056 1107 78-79 1.2431193245367567 1107 80-81 1.1143650677843198 1107 82-83 1.1475326535019654 1107 84-85 1.643929736532474 1107 86-87 1.950719939809641 1107 88-89 1.8020818092456494 1107 90-91 1.6473988174373844 1107 92-93 1.8831716361203803 1107 94-95 2.1536449310408337 1107 96-97 2.6546081127686314 1107 98-99 2.213293701324158 1107 100-101 1.6525121308643396 1108 1 0.10260652615733434 1108 2 -0.09915988333838754 1108 3 0.17247829974344597 1108 4 0.3388860628531134 1108 5 0.25655325533240614 1108 6 0.3387182842384462 1108 7 -0.11368252776343013 1108 8 -0.10223083159256419 1108 9 -0.07420833210930056 1108 10-11 0.27915481557139543 1108 12-13 0.543138266547885 1108 14-15 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0.09388206300968704 2316 44-45 0.5415033624104737 2316 46-47 0.024753663684471405 2316 48-49 0.6702437770123844 2316 50-51 0.9941752929577596 2316 52-53 0.7852570232345357 2316 54-55 0.02328605481538304 2316 56-57 1.0515038771507115 2316 58-59 0.619182772376174 2316 60-61 0.725897367654806 2316 62-63 0.20072687895275365 2316 64-65 -0.09525807334680536 2316 66-67 0.4187375081460516 2316 68-69 0.3823549160142683 2316 70-71 0.5285076881503095 2316 72-73 0.3926970826828473 2316 74-75 0.0864801564408495 2316 76-77 0.1300973421924212 2316 78-79 0.08462813419843229 2316 80-81 0.189702151083182 2316 82-83 -0.8548869014653597 2316 84-85 -0.024529193072954314 2316 86-87 -0.7814125773547218 2316 88-89 -0.13838059459255092 2316 90-91 0.36604800264708004 2316 92-93 0.00232954827191989 2316 94-95 -0.12762316392152684 2316 96-97 -0.9026546881247377 2316 98-99 -0.5229127673663854 2316 100-101 -0.8917429770850376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 10.0 6 44.0 7 88.0 8 196.0 9 354.0 10 613.0 11 977.0 12 1319.0 13 1819.0 14 2249.0 15 3163.0 16 4149.0 17 5224.0 18 4569.0 19 2971.0 20 2506.0 21 2451.0 22 2395.0 23 2527.0 24 2582.0 25 2456.0 26 2426.0 27 2602.0 28 2989.0 29 3608.0 30 4140.0 31 4757.0 32 5729.0 33 7079.0 34 8939.0 35 11858.0 36 16101.0 37 22852.0 38 10055.0 39 252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.26359932901989 25.43288487213721 16.789565574612304 17.513950224230598 2 15.901556249657514 24.22187517124226 40.725519206531864 19.15104937256836 3 17.155063811140934 28.94437749904146 38.29421591718245 15.606342772635154 4 10.692474119515802 24.151010571287728 38.98504683135236 26.171468477844112 5 7.854432373933175 42.74970204254969 36.84055509130512 12.555310492212007 6 20.73862080297968 44.12759489510872 18.741441638823463 16.39234266308813 7 19.591115736429863 43.90644684230706 19.550720271676617 16.95171714958646 8 24.851426849975354 35.100235526099574 20.5770389439667 19.47129867995837 9 25.51281700169798 17.18861258695295 23.599167442624747 33.69940296872432 10-11 21.905981267459058 30.09222490003834 28.392876704825547 19.608917127677056 12-13 23.737812893684616 31.556731664567018 27.9906337295284 16.714821712219972 14-15 21.28087856712494 30.623527961877635 30.061414799802815 18.03417867119461 16-17 15.559100618940679 34.90236621569809 30.359245768746234 19.179287396614995 18-19 14.986375089006957 30.708426904748865 38.08573423892206 16.21946376732212 20-21 16.180779974804185 28.9786109437476 38.74062003615052 16.099989045297693 22-23 16.550501177630498 30.222654324368737 37.89128827299118 15.335556225009586 24-25 15.820988520779341 32.04801210573345 38.507256250577726 13.623743122909485 26-27 15.706895902444703 31.519234785228395 39.4479991509786 13.3258701613483 28-29 14.702287828941737 31.320628117393728 40.032042613937854 13.94504143972668 30-31 16.20593682495233 30.43589892813984 39.49347675011725 13.864687496790582 32-33 14.65651520494062 30.697102793820115 40.39759131009315 14.248790691146112 34-35 13.877257553710521 32.17495506291193 39.63399811692202 14.313789266455535 36-37 15.753626364595174 30.926144068377003 37.795690824167544 15.524538742860274 38-39 13.57855203650892 31.787478645499895 38.75306837205959 15.880900945931604 40-41 14.087057736088052 31.736190753325005 39.16810735865528 15.00864415193167 42-43 15.030211738929495 31.171667722223173 38.725458311223704 15.07266222762363 44-45 15.245115325228442 31.54642281238124 37.862349231564565 15.346112630825747 46-47 16.54878950539519 31.25 35.52644191269102 16.67476858191379 48-49 15.715889795694801 31.068220408610397 36.280947034014346 16.934942761680453 50-51 14.825870987398627 31.68350387352753 35.10514838537405 18.38547675369979 52-53 16.281426302240238 32.293093060196085 34.5501040696719 16.875376567891767 54-55 16.16999133884954 31.286266427942994 33.65661448294986 18.88712775025761 56-57 17.09583995179931 32.94421317850688 32.59434737361012 17.365599496083696 58-59 16.37537239324727 32.80964284491319 32.683628394343046 18.13135636749649 60-61 15.830914169907434 32.864449252341565 32.626869146080956 18.677767431670045 62-63 15.532860410019037 32.905944865175776 32.62520370852221 18.935991016282987 64-65 15.30889306612705 32.715992316620955 32.49377694233014 19.481337674921846 66-67 15.911246229745043 33.2689693894669 31.371602491021573 19.448181889766474 68-69 15.503061098708448 31.30606878227165 32.204538917726985 20.98633120129292 70-71 15.536506545434628 31.87818371035767 32.90005203483595 19.685257709371747 72-73 16.69017363203155 30.044640411896808 33.33173577258038 19.933450183491264 74-75 15.593744970947053 30.933426467113843 32.58244222795177 20.890386333987323 76-77 15.2331535234181 30.797842281017214 33.6994605702543 20.269543625310384 78-79 14.611327991233477 30.75234572974454 33.00732826518731 21.628998013834668 80-81 14.742169549331754 29.33264391353032 33.44519283779573 22.4799936993422 82-83 14.90195944141195 30.25730938080008 32.60268061995329 22.238050557834686 84-85 14.526511131045721 29.96035142741709 32.704594167071825 22.80854327446536 86-87 14.478716228939629 30.568309041793775 32.03812693228156 22.91484779698504 88-89 15.105633561526496 30.43843238835324 31.24601689862879 23.20991715149148 90-91 15.042717482493536 29.033506257811563 32.43960484188539 23.48417141780951 92-93 15.284759413223028 28.486898875481103 32.5997479762769 23.62859373501897 94-95 14.812886965097801 28.66418278450496 32.594789326612236 23.928140923784998 96-97 16.187299217075022 28.25208403257735 31.078285647745407 24.482331102602213 98-99 15.476704276876912 28.333025052921474 30.355344554740327 25.834926115461293 100-101 15.669906835169126 28.547265487240765 31.65173267411483 24.131095003475277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 800.0 1 465.0 2 155.0 3 183.0 4 143.0 5 123.0 6 189.5 7 340.5 8 471.0 9 509.5 10 465.5 11 359.5 12 319.0 13 360.5 14 430.0 15 472.0 16 519.5 17 603.5 18 681.5 19 778.0 20 909.0 21 1030.5 22 1166.0 23 1294.5 24 1392.0 25 1833.0 26 2871.5 27 4111.5 28 4307.5 29 4473.0 30 5479.5 31 6209.5 32 6384.5 33 6478.5 34 7161.5 35 7825.5 36 8015.5 37 7842.0 38 6456.5 39 4993.5 40 4540.5 41 4478.0 42 4479.0 43 4616.0 44 4837.5 45 4350.0 46 3420.0 47 2700.5 48 2757.5 49 2757.5 50 2426.0 51 2093.5 52 1344.0 53 696.5 54 453.5 55 359.0 56 289.0 57 223.5 58 189.5 59 163.0 60 130.0 61 105.5 62 83.5 63 70.5 64 52.5 65 39.0 66 32.5 67 26.0 68 20.0 69 14.5 70 13.0 71 11.5 72 11.0 73 12.5 74 10.5 75 10.5 76 9.0 77 6.0 78 7.5 79 5.5 80 3.5 81 2.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.846688941228022E-4 2 0.04381880922385934 3 0.0 4 0.0 5 0.042449471435613735 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006504354494166621 26-27 0.0037656789176754124 28-29 3.423344470614011E-4 30-31 0.0010270033411842034 32-33 0.0010270033411842034 34-35 0.01266637454127184 36-37 0.028756093553157693 38-39 0.006504354494166621 40-41 0.0023963411294298078 42-43 0.0023963411294298078 44-45 0.008558361176535028 46-47 0.0 48-49 0.0 50-51 3.423344470614011E-4 52-53 0.0 54-55 0.0010270033411842034 56-57 0.0 58-59 0.028071424659034887 60-61 0.0 62-63 0.009585364517719231 64-65 0.018143725694254258 66-67 0.007873692282412225 68-69 0.020540066823684068 70-71 0.0 72-73 0.0 74-75 0.020197732376622667 76-77 0.046899819247411954 78-79 0.0314947691296489 80-81 0.027044421317850686 82-83 0.0308101002355261 84-85 0.01574738456482445 86-87 0.011981705647149038 88-89 0.08695294955359588 90-91 0.026359752423727886 92-93 0.026017417976666485 94-95 0.03286410691789451 96-97 0.044503478117982144 98-99 0.058196856000438196 100-101 0.016774387906008652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 146056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.761187489729966 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24220115947364 23.58342005805992 2 8.512008834084844 5.066549816508736 3 3.154044354467654 2.8160431615270856 4 1.7139044814576239 2.0403133044859505 5 1.1502714640655194 1.7116722353070057 6 0.8097911107021257 1.4460207043873583 7 0.5590319315358425 1.1646217889028865 8 0.4670102144106009 1.1119022840554307 9 0.3588846967884421 0.9612751273484142 >10 3.096530781264378 19.541819576053022 >50 0.5613324744639735 11.83313249712439 >100 0.34508143921965584 18.757873692282413 >500 0.018404343425048313 3.4623705975790107 >1k 0.011502714640655195 6.502985156378376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2494 1.7075642219422686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2458 1.6829161417538478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1763 1.2070712603385003 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1444 0.9886618831133265 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1339 0.9167716492304322 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 947 0.6483814427342938 TruSeq Adapter, Index 1 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 660 0.45188147012104946 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 645 0.44161143670920744 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 602 0.41217067426192694 RNA PCR Primer, Index 1 (95% over 23bp) GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 599 0.4101166675795585 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 557 0.38136057402640083 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 543 0.3717752095086816 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 504 0.3450731226378923 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 482 0.33001040696719064 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 477 0.32658706249657665 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 443 0.3033083200964014 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 442 0.3026236512022786 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 421 0.28824560442569974 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 403 0.2759215643314893 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 375 0.25675083529605086 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 366 0.2505888152489456 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 339 0.23210275510762995 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 334 0.2286794106370159 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 332 0.22731007284877033 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 331 0.2266254039546475 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 323 0.22114805280166513 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 320 0.21909404611929673 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 316 0.2163553705428055 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 309 0.2115626882839459 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.20540066823684067 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 289 0.19786931040148983 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 287 0.19649997261324423 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 282 0.1930766281426302 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 278 0.19033795256613903 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 276 0.1889686147778934 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 272 0.18622993920140218 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 271 0.1855452703072794 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 267 0.1828065947307882 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 262 0.17938325026017418 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 257 0.17595990578956017 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 247 0.16911321684833214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 247 0.16911321684833214 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 240 0.16432053458947254 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 239 0.16363586569534974 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 234 0.1602125212247357 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 234 0.1602125212247357 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 233 0.15952785233061292 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 233 0.15952785233061292 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 224 0.1533658322835077 TruSeq Adapter, Index 1 (96% over 26bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.14925781891877088 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 218 0.14925781891877088 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 218 0.14925781891877088 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 216 0.14788848113052527 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 215 0.14720381223640247 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 214 0.14651914334227967 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 211 0.1444651366599113 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 211 0.1444651366599113 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 207 0.14172646108342007 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 207 0.14172646108342007 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 200 0.13693377882456045 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 193 0.13214109656570083 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 190 0.13008708988333242 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 189 0.12940242098920962 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 187 0.12803308320096402 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 183 0.1252944076244728 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.1239250698362272 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 179 0.1225557320479816 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 179 0.1225557320479816 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 177 0.12118639425973599 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 177 0.12118639425973599 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 174 0.11913238757736758 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 171 0.11707838089499917 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 170 0.11639371200087639 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 170 0.11639371200087639 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 169 0.11570904310675358 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 168 0.11502437421263077 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 164 0.11228569863613955 TruSeq Adapter, Index 1 (95% over 24bp) GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT 160 0.10954702305964836 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 159 0.10886235416552555 No Hit CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT 158 0.10817768527140274 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 153 0.10475434080078874 No Hit AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA 153 0.10475434080078874 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 152 0.10406967190666594 No Hit CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA 151 0.10338500301254315 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 150 0.10270033411842033 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 150 0.10270033411842033 No Hit AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT 150 0.10270033411842033 No Hit AGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGA 149 0.10201566522429753 No Hit AATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT 148 0.10133099633017471 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 148 0.10133099633017471 No Hit GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG 148 0.10133099633017471 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 148 0.10133099633017471 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 147 0.10064632743605192 No Hit CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA 147 0.10064632743605192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.846688941228022E-4 0.0 10-11 0.0 0.0 0.0 6.846688941228022E-4 0.0 12-13 0.0 0.0 0.0 6.846688941228022E-4 0.0 14-15 0.0 0.0 0.0 6.846688941228022E-4 0.0 16-17 0.0 0.0 0.0 6.846688941228022E-4 0.0 18-19 0.0 0.0 0.0 0.0010270033411842034 0.0 20-21 0.0 0.0 0.0 0.00308101002355261 0.0 22-23 0.0 0.0 0.0 0.014035712329517445 0.0 24-25 0.0 0.0 0.0 0.0308101002355261 0.0 26-27 0.0 0.0 0.0 0.06435887604754341 0.0 28-29 0.0 0.0 0.0 0.13761844771868323 0.0 30-31 0.0 0.0 0.0 0.29988497562578736 0.0 32-33 0.0 0.0 0.0 0.5111053294626718 0.0 34-35 0.0 0.0 0.0 0.8441967464534151 0.0 36-37 0.0 0.0 0.0 1.268349126362491 0.0 38-39 0.0 0.0 0.0 1.7568603823191105 0.0 40-41 0.0 0.0 0.0 2.223119899216739 0.0 42-43 0.0 0.0 0.0 2.661650325902394 0.0 44-45 0.0 0.0 0.0 3.1474229062825216 0.0 46-47 0.0 0.0 0.0 3.6797529714630004 0.0 48-49 0.0 0.0 0.0 4.2028400065728215 0.0 50-51 0.0 0.0 0.0 4.733458399517993 0.0 52-53 0.0 0.0 0.0 5.1853398696390425 0.0 54-55 0.0 0.0 0.0 5.698841540231144 0.0 56-57 0.0 0.0 0.0 6.3191515583064035 0.0 58-59 0.0 0.0 0.0 6.902831790546092 0.0 60-61 0.0 0.0 0.0 7.536492852056745 0.0 62-63 0.0 0.0 0.0 8.131127786602399 0.0 64-65 0.0 0.0 0.0 8.674412554088843 0.0 66-67 0.0 0.0 0.0 9.329983020211426 0.0 68-69 0.0 0.0 0.0 10.055047379087473 0.0 70-71 0.0 0.0 0.0 10.875280714246589 0.0 72-73 0.0 0.0 0.0 11.653406912417156 0.0 74-75 0.0 0.0 0.0 12.409966040422852 0.0 76-77 0.0 0.0 0.0 13.300377937229555 0.0 78-79 0.0 0.0 0.0 14.156214054883058 0.0 80-81 0.0 0.0 0.0 15.122281864490333 0.0 82-83 0.0 0.0 0.0 15.947307881908309 0.0 84-85 0.0 0.0 0.0 16.761721531467384 0.0 86-87 0.0 0.0 0.0 17.637413047050444 0.0 88-89 0.0 0.0 0.0 18.519608917127677 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1555 0.0 41.224567 1 CCATAGG 105 4.6233254E-8 36.178738 1 CATAGGG 110 6.9612724E-8 34.53425 2 AGGGTCT 125 5.5988494E-9 34.18891 5 ATAGGGT 115 1.0288204E-7 33.03276 3 TAGGGTC 115 1.0288204E-7 33.03276 4 GTATCAA 2355 0.0 32.05966 1 GTTGTGG 30 0.003945821 31.667238 64-65 GTAATGC 80 4.483178E-4 29.67787 4 TGTAATG 80 4.483178E-4 29.67787 3 TAATGCT 80 4.483178E-4 29.67787 5 AATGCTA 85 6.3923415E-4 27.932114 6 CAACCAC 35 0.008366769 27.152645 82-83 TATCAAC 2875 0.0 26.591373 2 GGTCTTC 150 1.0525146E-6 25.325117 7 TCAACGC 3030 0.0 25.231089 4 AACGCAG 3115 0.0 24.542599 6 GTAAGGT 155 1.4005564E-6 24.508177 4 CAACGCA 3125 0.0 24.464064 5 ATCAACG 3190 0.0 23.965578 3 >>END_MODULE