##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149240 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.659756097560976 31.0 31.0 34.0 27.0 34.0 2 30.715572232645403 31.0 31.0 34.0 27.0 34.0 3 30.64879388903779 31.0 31.0 34.0 27.0 34.0 4 34.276420530688824 37.0 35.0 37.0 30.0 37.0 5 34.35990351112302 37.0 35.0 37.0 32.0 37.0 6 34.45745108549987 37.0 35.0 37.0 32.0 37.0 7 34.413434735995715 37.0 35.0 37.0 31.0 37.0 8 34.40832216563924 37.0 35.0 37.0 31.0 37.0 9 35.80194987938891 39.0 35.0 39.0 30.0 39.0 10-11 35.714506834628786 39.0 35.0 39.0 30.0 39.0 12-13 35.61103256499598 39.0 35.0 39.0 30.0 39.0 14-15 36.449718574108815 39.0 36.0 41.0 30.0 41.0 16-17 36.350067006164565 39.0 36.0 41.0 30.0 41.0 18-19 36.52950616456714 39.5 36.0 41.0 30.5 41.0 20-21 36.67634012329134 40.0 36.0 41.0 31.0 41.0 22-23 36.55106539801662 40.0 36.0 41.0 30.0 41.0 24-25 36.419337979094074 39.5 36.0 41.0 30.0 41.0 26-27 36.18591195389976 39.0 35.5 41.0 30.0 41.0 28-29 35.935942106673814 39.0 35.0 41.0 29.0 41.0 30-31 35.48469579201287 38.5 35.0 40.0 27.5 41.0 32-33 34.95187617260788 38.0 35.0 40.0 27.0 41.0 34-35 34.569341329402306 38.0 35.0 40.0 25.0 41.0 36-37 34.19148016617529 38.0 34.5 40.0 23.5 41.0 38-39 33.785148083623696 38.0 34.0 40.0 20.5 41.0 40-41 33.35369203966765 38.0 33.0 40.0 12.5 41.0 42-43 32.8257672205843 38.0 33.0 40.0 4.5 41.0 44-45 32.06730769230769 37.5 32.5 40.0 2.0 41.0 46-47 31.10510922004824 37.0 31.0 40.0 2.0 41.0 48-49 30.310593674618065 37.0 29.5 40.0 2.0 41.0 50-51 29.36141450013401 36.0 26.5 39.5 2.0 40.5 52-53 29.300130662020905 36.5 27.0 39.5 2.0 40.5 54-55 29.273988206915035 37.0 25.5 40.0 2.0 41.0 56-57 28.93467904047172 36.0 23.5 40.0 2.0 41.0 58-59 28.561169927633344 36.0 22.0 40.0 2.0 41.0 60-61 28.15217770034843 36.0 19.0 39.5 2.0 41.0 62-63 27.63816671133744 35.0 10.5 39.0 2.0 40.5 64-65 27.146813856874832 35.0 4.5 39.0 2.0 40.0 66-67 26.589346019833826 35.0 2.0 38.5 2.0 40.0 68-69 26.02262798177432 34.0 2.0 37.5 2.0 40.0 70-71 25.459474671669792 34.0 2.0 37.0 2.0 39.0 72-73 24.963615652640044 33.0 2.0 36.5 2.0 39.0 74-75 24.460369203966764 33.0 2.0 36.0 2.0 38.5 76-77 23.84053537925489 32.5 2.0 35.0 2.0 37.5 78-79 23.20758844813723 31.5 2.0 35.0 2.0 37.0 80-81 22.615240552130796 31.0 2.0 35.0 2.0 36.0 82-83 22.05176896274457 31.0 2.0 35.0 2.0 36.0 84-85 21.546281157866524 30.0 2.0 34.5 2.0 35.5 86-87 21.013632404181184 29.5 2.0 34.0 2.0 35.0 88-89 20.326242964352723 29.0 2.0 34.0 2.0 35.0 90-91 19.67258777807558 27.5 2.0 34.0 2.0 35.0 92-93 19.076899624765478 26.0 2.0 34.0 2.0 35.0 94-95 18.346421870812115 25.0 2.0 34.0 2.0 35.0 96-97 17.412325783972125 21.0 2.0 33.5 2.0 35.0 98-99 16.296029884749395 2.0 2.0 33.0 2.0 35.0 100-101 13.508127847761994 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17487050813975813 1101 2 0.2974229706690181 1101 3 0.5686398428391222 1101 4 -0.030513159561820657 1101 5 0.36184940535465415 1101 6 0.2872913441618721 1101 7 0.11961935451027017 1101 8 0.007347478235224969 1101 9 0.2233495841907711 1101 10-11 0.11921093182706954 1101 12-13 0.2324663432669638 1101 14-15 0.4071582847033923 1101 16-17 0.2231889965901317 1101 18-19 0.6367087768912256 1101 20-21 0.4245670576178995 1101 22-23 0.5059073955022697 1101 24-25 0.8022803232222628 1101 26-27 0.4975290269923178 1101 28-29 0.5452797009851764 1101 30-31 0.5833391651954472 1101 32-33 0.07286660437186043 1101 34-35 0.41305817327941696 1101 36-37 0.04539190416486605 1101 38-39 0.5090459549071298 1101 40-41 -0.30231623805573093 1101 42-43 0.2579420272507278 1101 44-45 -0.08767774014942376 1101 46-47 -0.5123579747916054 1101 48-49 -0.6917594026238696 1101 50-51 -0.5959427463012474 1101 52-53 -0.6398880775220412 1101 54-55 -1.0260283417444747 1101 56-57 -1.2091025218297986 1101 58-59 -1.5848529580309823 1101 60-61 -1.5023416571914048 1101 62-63 -1.376558382643772 1101 64-65 -0.7990398569032244 1101 66-67 -0.5683163055394154 1101 68-69 -0.9155871496352894 1101 70-71 -1.0484911812006636 1101 72-73 -1.014557703647558 1101 74-75 -0.8657980727504224 1101 76-77 -0.68118634714493 1101 78-79 -1.2891595559311675 1101 80-81 -0.5568692810114655 1101 82-83 -0.3961260475490036 1101 84-85 0.04216249977974229 1101 86-87 -0.12670395760259012 1101 88-89 8.537659246776741E-4 1101 90-91 0.31056930467274313 1101 92-93 1.0722009615739267 1101 94-95 0.00886422896209993 1101 96-97 -0.2651852393894494 1101 98-99 -0.17346901337162635 1101 100-101 0.3050252021792339 1102 1 0.46460893872325926 1102 2 0.796517538073445 1102 3 0.40234205611880114 1102 4 0.3384003213234905 1102 5 0.3435395461997288 1102 6 0.29030945281378706 1102 7 0.4355147267436692 1102 8 0.5855164923197265 1102 9 0.6520216163839265 1102 10-11 0.7126716964145956 1102 12-13 0.52653072958487 1102 14-15 0.687037560357318 1102 16-17 0.17243446942715224 1102 18-19 0.735753042484788 1102 20-21 0.792172691420717 1102 22-23 0.839207194295021 1102 24-25 0.7740610073268996 1102 26-27 1.339832849929941 1102 28-29 0.9705312100395034 1102 30-31 0.85029087545702 1102 32-33 0.4923334051766872 1102 34-35 0.34665978293737254 1102 36-37 0.4299492482292564 1102 38-39 0.7760982687904274 1102 40-41 0.7494443253245464 1102 42-43 1.2593504779549463 1102 44-45 0.9326944932509775 1102 46-47 0.20338649200920145 1102 48-49 -0.5077050766681346 1102 50-51 -0.07979586501351932 1102 52-53 -0.3695661459325059 1102 54-55 -0.9712999715231447 1102 56-57 -0.7980361234394557 1102 58-59 -0.297730221612472 1102 60-61 -1.0625529247970356 1102 62-63 -0.632343090893265 1102 64-65 -0.468255148653725 1102 66-67 -0.41947324718931256 1102 68-69 -0.5040680349471636 1102 70-71 -0.7643362516232024 1102 72-73 -0.5962981463034929 1102 74-75 -0.3123775496115897 1102 76-77 0.13143940738223492 1102 78-79 -0.23292213138388718 1102 80-81 -0.3125030838283678 1102 82-83 -0.2452709167642908 1102 84-85 -0.08142904951603214 1102 86-87 -0.14149268999695508 1102 88-89 -0.3963796344777357 1102 90-91 -0.2300242566954651 1102 92-93 -0.14676282112224825 1102 94-95 -0.6851498555449389 1102 96-97 -0.8538170301339179 1102 98-99 -0.5509841039148888 1102 100-101 -0.5168057837362579 1103 1 0.7736963398423029 1103 2 0.34957561254768876 1103 3 0.37793534186064903 1103 4 0.4637138463090196 1103 5 0.02075351494369926 1103 6 0.6591113050229325 1103 7 0.3906565365063557 1103 8 0.7970735056324827 1103 9 0.5426255137406741 1103 10-11 0.5460211079523134 1103 12-13 0.8096679088246859 1103 14-15 1.2100937054470364 1103 16-17 0.9997545543200701 1103 18-19 0.8161119080066896 1103 20-21 0.8978547288507741 1103 22-23 1.1085492741715441 1103 24-25 0.8226814973905618 1103 26-27 1.2737031952897766 1103 28-29 1.320328624664235 1103 30-31 1.434170867739482 1103 32-33 1.505694393021571 1103 34-35 1.9727450617334341 1103 36-37 1.8455386752020502 1103 38-39 2.3841438022652497 1103 40-41 2.2596700633141253 1103 42-43 2.8236954519082573 1103 44-45 2.8656001463476386 1103 46-47 2.401389579024439 1103 48-49 1.2939059594113544 1103 50-51 1.6566502086889692 1103 52-53 1.4810512571159258 1103 54-55 1.6370832042437833 1103 56-57 1.4423651885420234 1103 58-59 1.4686695468613848 1103 60-61 1.4692336852743502 1103 62-63 1.109987605614549 1103 64-65 0.6592771685370913 1103 66-67 1.1296778236190974 1103 68-69 0.675557664454729 1103 70-71 1.0100704626349497 1103 72-73 1.0996790869590924 1103 74-75 1.1154152344902855 1103 76-77 0.6967490735987134 1103 78-79 0.34753320336432836 1103 80-81 0.13217181487894436 1103 82-83 -0.3429460084100562 1103 84-85 -0.6095498289873795 1103 86-87 -0.2760190260957387 1103 88-89 0.21166589899708654 1103 90-91 0.4729469954750911 1103 92-93 0.5805669106932996 1103 94-95 1.0866039549894992 1103 96-97 1.9351083026849132 1103 98-99 1.701041749837767 1103 100-101 1.5611895857408786 1104 1 0.7367449394409427 1104 2 0.8127550907782108 1104 3 0.47168806849881406 1104 4 0.9425987603653851 1104 5 0.7593016346540225 1104 6 0.6608127272373991 1104 7 0.5609296365848877 1104 8 0.7165831470250339 1104 9 0.3501466724528228 1104 10-11 0.6816768098980504 1104 12-13 1.0361970075071767 1104 14-15 0.8852747542211361 1104 16-17 0.6896812622862214 1104 18-19 0.7596159652442793 1104 20-21 0.45609572003828447 1104 22-23 0.4520402435459445 1104 24-25 0.9436133532495674 1104 26-27 0.7692533218057847 1104 28-29 0.6250795190015523 1104 30-31 1.062388300773243 1104 32-33 0.25150627680134363 1104 34-35 0.2067342424331997 1104 36-37 0.5081990015261013 1104 38-39 0.7229449539972208 1104 40-41 0.896569112262732 1104 42-43 0.4009356578239718 1104 44-45 0.8555661179033507 1104 46-47 0.3787475757088501 1104 48-49 -0.10320162282405221 1104 50-51 -0.19155239143318425 1104 52-53 -0.2655022722445146 1104 54-55 0.40267957454306114 1104 56-57 0.5880767320373543 1104 58-59 0.7085046397852146 1104 60-61 0.4424035657385339 1104 62-63 0.1681413443807962 1104 64-65 -0.5712873546284278 1104 66-67 -0.6676566149115715 1104 68-69 -0.726392427160313 1104 70-71 -0.8941918181433444 1104 72-73 -0.8738616162954216 1104 74-75 -1.1079727770270367 1104 76-77 -0.6853947183915174 1104 78-79 -0.8122259799530092 1104 80-81 -0.7291622746420359 1104 82-83 -0.8987875580130584 1104 84-85 -1.2200986467170694 1104 86-87 -0.7085510913605475 1104 88-89 -1.010042503411082 1104 90-91 -0.7483251448268007 1104 92-93 0.02099532008165994 1104 94-95 -0.041613478044265406 1104 96-97 -0.019825821737040172 1104 98-99 0.2713672013690456 1104 100-101 0.6238150110964309 1105 1 0.4162337200912063 1105 2 0.6841176485401661 1105 3 0.4118237830818856 1105 4 0.4466577087855157 1105 5 0.4703461373808011 1105 6 0.6453679081207895 1105 7 0.9730273843888924 1105 8 0.5769553213724805 1105 9 0.5708285757874094 1105 10-11 0.7222454789792607 1105 12-13 0.9263972489625729 1105 14-15 0.9174757450208517 1105 16-17 0.7830162608291644 1105 18-19 0.7358450980863722 1105 20-21 1.0262010445460064 1105 22-23 1.1195395150167329 1105 24-25 0.9310618358273075 1105 26-27 0.78149261242649 1105 28-29 0.886829654299838 1105 30-31 1.2166491558583772 1105 32-33 1.3860552131487012 1105 34-35 1.1121711518041693 1105 36-37 1.7378522216251824 1105 38-39 1.9387144889874293 1105 40-41 1.537716441682825 1105 42-43 1.581798236401049 1105 44-45 0.8652867789915675 1105 46-47 0.6715122399609612 1105 48-49 0.3483199624554949 1105 50-51 0.514538112709026 1105 52-53 0.8536226601063426 1105 54-55 0.6946532642312491 1105 56-57 1.1007947704204355 1105 58-59 1.4348482557878803 1105 60-61 1.4873642251615387 1105 62-63 1.1898515146484812 1105 64-65 1.042883579137861 1105 66-67 1.066534301370016 1105 68-69 1.364361496489824 1105 70-71 1.7588617599758045 1105 72-73 1.7031126978463718 1105 74-75 1.2734642988648943 1105 76-77 1.2341964726496535 1105 78-79 1.785396933331203 1105 80-81 2.0905163399035693 1105 82-83 2.118509806792737 1105 84-85 1.776966421348373 1105 86-87 2.022642447626172 1105 88-89 2.867543775261744 1105 90-91 2.6718904998174686 1105 92-93 2.5437462463545053 1105 94-95 1.8629491962823614 1105 96-97 2.559604676576935 1105 98-99 2.111194852174588 1105 100-101 1.6053519995648546 1106 1 0.6635176226146875 1106 2 0.895388912012443 1106 3 0.6456029459611656 1106 4 0.8212843806463184 1106 5 0.7936374848248562 1106 6 0.18369626374560255 1106 7 0.6938861473201143 1106 8 0.38516204777164376 1106 9 0.8345605586467784 1106 10-11 0.6092298533029421 1106 12-13 0.9570642619046126 1106 14-15 0.9855404985160732 1106 16-17 0.9639884289458536 1106 18-19 1.2069311042327584 1106 20-21 1.0096663953662457 1106 22-23 1.3142328448873215 1106 24-25 1.3334771065713724 1106 26-27 1.3739481376829517 1106 28-29 1.067607042517814 1106 30-31 0.7852549646278035 1106 32-33 0.8500425740975004 1106 34-35 0.9587062338281456 1106 36-37 0.7165130016104726 1106 38-39 0.5210847439326827 1106 40-41 0.15910760300386784 1106 42-43 0.18410096764807093 1106 44-45 1.06404884452418 1106 46-47 0.08695612170792089 1106 48-49 1.0234487336107598 1106 50-51 0.9412143019485164 1106 52-53 1.1291280057324542 1106 54-55 1.3414740234400107 1106 56-57 1.4600554724937602 1106 58-59 0.8141063607958863 1106 60-61 1.4478239057225046 1106 62-63 1.2690187223704186 1106 64-65 1.8654823758309327 1106 66-67 0.5641103737415669 1106 68-69 0.4347203243476834 1106 70-71 0.3235286863490039 1106 72-73 1.385513251886973 1106 74-75 1.2383173482524157 1106 76-77 1.3000104362041718 1106 78-79 0.7334989114292831 1106 80-81 0.2633293944852255 1106 82-83 0.48015115207257253 1106 84-85 0.4790830295810693 1106 86-87 0.4425013404106579 1106 88-89 1.5373485625188117 1106 90-91 1.436704593225251 1106 92-93 1.2128584828605895 1106 94-95 0.5434668779687222 1106 96-97 0.7068582800050613 1106 98-99 0.3275953196463455 1106 100-101 0.09409804986135306 1107 1 0.036107996765782246 1107 2 0.035685027083943055 1107 3 0.025828195887349636 1107 4 -0.26759232243716013 1107 5 0.026817558220898263 1107 6 0.1257808801036404 1107 7 0.07685320346386959 1107 8 -0.04137863641445705 1107 9 0.23549699092416176 1107 10-11 0.2676640710445426 1107 12-13 0.020273440628194805 1107 14-15 0.44896902173706366 1107 16-17 0.2183436826683831 1107 18-19 0.4029280671551021 1107 20-21 0.38284731239497205 1107 22-23 0.43293196329116057 1107 24-25 0.21749870356803314 1107 26-27 0.355240582952284 1107 28-29 0.18558907314167783 1107 30-31 0.0910507211554119 1107 32-33 0.3884489519605765 1107 34-35 0.4551418857452987 1107 36-37 0.18654295057068992 1107 38-39 0.5446010049526322 1107 40-41 0.17363917595699974 1107 42-43 0.6780057215182396 1107 44-45 0.7727635701326463 1107 46-47 1.0347985311228598 1107 48-49 1.5210021988319973 1107 50-51 1.391250156337513 1107 52-53 1.409656963424272 1107 54-55 1.3262464535239538 1107 56-57 1.0243824645214303 1107 58-59 1.4887594168552774 1107 60-61 1.6175628014073204 1107 62-63 1.6904343380113716 1107 64-65 1.3618336644798532 1107 66-67 1.6667382895470269 1107 68-69 1.0188305027759945 1107 70-71 1.0230056340859583 1107 72-73 1.2184950716026677 1107 74-75 1.9748297707436677 1107 76-77 2.1075989121817322 1107 78-79 1.8884109627221513 1107 80-81 1.5001370884152863 1107 82-83 0.8018493846620984 1107 84-85 0.7799013532829271 1107 86-87 0.7373087812509134 1107 88-89 0.7733969870347757 1107 90-91 0.2644137086763827 1107 92-93 0.8426513710370749 1107 94-95 1.1208069041213342 1107 96-97 1.2413206750782457 1107 98-99 1.7490742077384702 1107 100-101 0.830821380523183 1108 1 -0.19525936199011085 1108 2 -0.1759536527076051 1108 3 -0.24499652079724044 1108 4 -0.3051884842371422 1108 5 -0.08750124399599457 1108 6 -0.23868268181215058 1108 7 0.01572325061416535 1108 8 -0.3842109633232198 1108 9 -0.3084685976274102 1108 10-11 0.36856158117772253 1108 12-13 0.055842966643588454 1108 14-15 -0.2051793776342663 1108 16-17 -0.04272009431895896 1108 18-19 -0.9382912231087772 1108 20-21 -0.5403680073171628 1108 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-1.1337517407040885 2316 76-77 -1.3327307486893325 2316 78-79 -1.2794105211821325 2316 80-81 -0.01768009182227459 2316 82-83 -0.32392527534823046 2316 84-85 -0.38148614886890186 2316 86-87 -0.45035260625123286 2316 88-89 -0.40542036534945325 2316 90-91 -1.5850870659836254 2316 92-93 -1.453957339584374 2316 94-95 -1.6343790142811407 2316 96-97 -2.5952045444087553 2316 98-99 -2.372310712213327 2316 100-101 -2.183488311334278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3087.0 3 532.0 4 426.0 5 318.0 6 365.0 7 406.0 8 505.0 9 584.0 10 758.0 11 1062.0 12 1576.0 13 2025.0 14 2451.0 15 3423.0 16 4108.0 17 5147.0 18 4098.0 19 2491.0 20 2394.0 21 2325.0 22 2141.0 23 2243.0 24 2304.0 25 2484.0 26 2661.0 27 2937.0 28 3405.0 29 3842.0 30 4368.0 31 5091.0 32 5935.0 33 7236.0 34 9099.0 35 11756.0 36 14979.0 37 20394.0 38 9815.0 39 469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83585195727626 24.273448042052404 16.191921158455123 16.69877884221621 2 16.197371949584337 23.740949316170557 40.83735585947975 19.224322874765353 3 18.077902923035666 28.582059533386968 36.971037811745774 16.36899973183159 4 11.237898575987556 22.756711094424627 40.17538684330499 25.830003486282816 5 8.759001729941398 41.924928589628394 36.471590070941005 12.844479609489198 6 21.699334133535395 43.65884569734927 19.234354149762957 15.407466019352373 7 20.47835792380863 43.617313660953094 20.55008479859496 15.354243616643318 8 25.24567307047767 34.32786797334799 20.70289981365044 19.723559142523897 9 26.66693482428972 17.42823431613102 24.418433155008515 31.486397704570745 10-11 22.153581089461035 29.94325095280075 28.60916230629737 19.29400565144085 12-13 24.229473578359897 31.78641460103575 28.287998391070442 15.696113429533915 14-15 21.65593567437667 30.555341419842673 31.16166203574912 16.627060870031542 16-17 15.652785923753665 34.643988269794725 30.984499371596147 18.71872643485547 18-19 15.055724077829357 30.895439843134625 38.804404297038666 15.244431781997353 20-21 16.613030827198735 29.086045295466427 39.398205881169794 14.902717996165038 22-23 17.07003124120084 29.408629543717566 38.44209651251659 15.079242702564995 24-25 16.08370271520825 31.33554180106355 38.811249773676764 13.769505710051433 26-27 15.577680561841067 30.97836107339803 39.56118737533732 13.882770989423577 28-29 15.015619972111981 31.33949640673603 39.795063284350526 13.849820336801457 30-31 16.237562689409177 30.02688604607504 39.627081824764666 14.10846943975112 32-33 14.70478795619337 30.929131007572636 40.30692800466627 14.059153031567725 34-35 14.83633072423445 31.937046410673283 38.714110923017614 14.512511942074651 36-37 16.036243203561302 30.65520685979391 37.68327757627766 15.625272360367127 38-39 14.146874403048349 32.09748283292726 38.47669668321095 15.27894608081343 40-41 14.402866028579856 31.805142229014184 39.01679669696239 14.775195045443578 42-43 14.911998498679644 31.724775807294808 38.14426080079355 15.218964893231995 44-45 15.82662945329174 31.69715103247012 37.27949232557906 15.196727188659079 46-47 16.43629978856509 32.03724739224694 35.60148373023452 15.924969088953448 48-49 16.073798067257304 31.58901741076814 36.13639105201785 16.200793469956707 50-51 15.492098808454744 32.269900414159146 34.452612955541554 17.78538782184455 52-53 16.738093881831798 32.471962444586666 33.99254118932177 16.797402484259766 54-55 16.715754767241986 31.49043535951589 33.70760915819771 18.086200715044416 56-57 17.109006168516775 32.492771007636094 33.5643036880426 16.833919135804535 58-59 17.17452637363374 31.381641994089303 33.25068187453509 18.19314975774187 60-61 16.270669637621673 30.766472884451073 34.616093417547624 18.346764060379634 62-63 16.61283513479103 30.47255597027427 34.22546253794453 18.689146356990168 64-65 16.2249913720024 30.57929883697943 34.16418995667572 19.031519834342447 66-67 16.733006325721025 31.463761123619598 32.93797576927201 18.86525678138737 68-69 16.523705813369073 29.954096163511473 33.50175908862456 20.02043893449489 70-71 16.36450157840765 30.942822099047596 33.99407510673521 18.698601215809546 72-73 17.490859982641506 29.31072037746345 34.54775763790451 18.650662001990533 74-75 16.418355584150984 30.372366092970886 34.095356817328835 19.1139215055493 76-77 16.587531623301558 30.46023422185736 34.05498684805737 18.897247306783722 78-79 16.071769613509574 30.548993985692984 33.46166088690087 19.91757551389657 80-81 16.504275337068112 29.3249256171764 33.67598573994157 20.49481330581392 82-83 16.59831534085198 30.231724396732538 33.035468977209526 20.134491285205957 84-85 16.455747097552393 30.022281415958858 32.98889279790923 20.53307868857952 86-87 16.899034675941284 30.642421318215174 32.14217504498256 20.316368960860984 88-89 17.579379176748954 30.560899262009933 31.68598221048469 20.173739350756424 90-91 17.83354951585171 29.463498671064052 32.06506255886365 20.63788925422059 92-93 18.38413694186405 29.036809158054183 32.43522204796182 20.143831852119945 94-95 18.374590279313882 29.371862159571542 31.950505070817165 20.30304249029741 96-97 19.870359694869354 28.687107023635562 30.596519687361745 20.84601359413334 98-99 19.473199175617033 28.70762889361773 29.62651430103383 22.192657629731404 100-101 20.560518785162422 28.798212566382965 30.593026727634438 20.04824192082018 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 811.0 1 487.5 2 190.5 3 200.5 4 152.5 5 137.5 6 209.0 7 372.0 8 540.0 9 577.5 10 480.0 11 392.5 12 375.5 13 411.0 14 457.0 15 537.0 16 619.0 17 695.0 18 815.5 19 933.5 20 1090.0 21 1244.0 22 1356.0 23 1453.5 24 1650.5 25 2093.5 26 3031.5 27 4268.5 28 4574.0 29 4757.5 30 5737.0 31 6399.0 32 6427.5 33 6551.0 34 7202.5 35 7769.5 36 8119.5 37 7940.0 38 6364.5 39 4907.5 40 4388.5 41 4237.0 42 4206.5 43 4398.0 44 4648.0 45 3960.5 46 2906.5 47 2262.0 48 2248.5 49 2306.0 50 2068.5 51 1725.5 52 1347.5 53 1045.5 54 791.0 55 627.5 56 514.0 57 437.5 58 417.5 59 369.0 60 303.0 61 259.5 62 232.5 63 209.0 64 176.0 65 173.0 66 172.5 67 146.5 68 123.0 69 102.0 70 88.5 71 73.0 72 66.5 73 61.0 74 46.0 75 40.0 76 34.0 77 24.5 78 18.5 79 16.0 80 14.0 81 11.5 82 6.5 83 4.5 84 3.0 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1896274457250067 2 0.05360493165371214 3 0.05360493165371214 4 0.05628517823639775 5 0.06834628785848298 6 0.0743768426695256 7 0.04221388367729831 8 0.038863575448941305 9 0.04958456177968373 10-11 0.049919592602519436 12-13 0.048579469311176626 14-15 0.04120879120879121 16-17 0.03517823639774859 18-19 0.045229161082819616 20-21 0.05762530152774055 22-23 0.05226480836236934 24-25 0.078397212543554 26-27 0.05863039399624766 28-29 0.048244438488340924 30-31 0.061645671401768964 32-33 0.056620209059233456 34-35 0.055280085767890653 36-37 0.054274993299383546 38-39 0.02981774323237738 40-41 0.02948271240954168 42-43 0.025462342535513268 44-45 0.007035647279549718 46-47 0.014406325381935137 48-49 0.01474135620477084 50-51 0.01474135620477084 52-53 0.013736263736263736 54-55 0.012396140444920933 56-57 0.009715893862235326 58-59 0.012731171267756634 60-61 0.01172607879924953 62-63 0.0060305548110426155 64-65 0.011056017153578129 66-67 0.005360493165371214 68-69 0.010050924685071027 70-71 0.02613240418118467 72-73 0.02244706512999196 74-75 0.021441972661484857 76-77 0.01507638702760654 78-79 0.008375770570892522 80-81 0.008040739748056821 82-83 0.00737067810238542 84-85 0.008375770570892522 86-87 0.010385955507906728 88-89 0.03417314392924149 90-91 0.04053872956311981 92-93 0.025462342535513268 94-95 0.035513267220584295 96-97 0.038863575448941305 98-99 0.025127311712677566 100-101 0.13367729831144465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149240.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.122219244170466 #Duplication Level Percentage of deduplicated Percentage of total 1 82.46210038488728 28.137898686679176 2 7.171471212002199 4.8941302599839185 3 2.6843924279318196 2.7479228088984184 4 1.4551095750530203 1.986062717770035 5 0.9740004712905506 1.6617528812650766 6 0.6558793496190402 1.342803537925489 7 0.45361715497604277 1.0834896810506567 8 0.400596968030791 1.0935406057357278 9 0.29062917288508366 0.8925221120343071 >10 2.6942109810698294 19.576521039935674 >50 0.4359437593276255 10.448941302599838 >100 0.306338857905899 19.12690967569016 >500 0.007854842510407667 1.6269096756901635 >1k 0.007854842510407667 5.380595014741356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2682 1.7971053336906997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 1.6195389975877783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1625 1.088850174216028 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1306 0.8751005092468508 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 685 0.45899222728491024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 669 0.4482712409541678 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 556 0.3725542749932994 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 518 0.34709193245778613 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 496 0.3323505762530153 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 492 0.32967032967032966 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 457 0.3062181720718306 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 450 0.30152774055213083 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 444 0.2975073706781024 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 0.28946663093004554 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 413 0.2767354596622889 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 386 0.2586437952291611 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 379 0.25395336370946126 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 367 0.24591262396140445 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 355 0.23787188421334765 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 351 0.23519163763066203 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.2338515143393192 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 347 0.2325113910479764 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 331 0.221790404717234 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 321 0.21508978826051994 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 311 0.20838917180380595 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 307 0.20570892522112036 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 304 0.20369874028410614 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 303 0.20302867863843474 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 293 0.19632806218172072 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 284 0.1902975073706781 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 277 0.1856070758509783 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 276 0.1849370142053069 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 273 0.1829268292682927 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.17086571964620745 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 253 0.16952559635486467 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 252 0.16885553470919323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 251 0.16818547306352186 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 246 0.16483516483516483 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 240 0.16081479496113643 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 234 0.156794425087108 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.156794425087108 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 233 0.15612436344143663 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 230 0.1541141785044224 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 213 0.14272313052800858 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 211 0.14138300723666578 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 208 0.13937282229965156 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 207 0.13870276065398018 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 206 0.13803269900830875 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 205 0.13736263736263737 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 205 0.13736263736263737 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 204 0.13669257571696597 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 204 0.13669257571696597 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 198 0.13267220584293754 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 196 0.13133208255159476 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 194 0.12999195926025195 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 190 0.12731171267756633 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 188 0.12597158938622352 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 186 0.12463146609488072 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 184 0.12329134280353793 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 180 0.12061109622085232 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 178 0.11927097292950951 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 177 0.11860091128383812 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 175 0.11726078799249531 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 172 0.1152506030554811 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 172 0.1152506030554811 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 171 0.11458054140980971 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 171 0.11458054140980971 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 171 0.11458054140980971 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 168 0.1125703564727955 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 167 0.1119002948271241 No Hit GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG 167 0.1119002948271241 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 166 0.1112302331814527 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 166 0.1112302331814527 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 165 0.11056017153578131 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 162 0.10854998659876709 No Hit GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC 160 0.10720986330742428 No Hit AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA 159 0.10653980166175289 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 157 0.10519967837041008 No Hit ATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTG 156 0.10452961672473868 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 156 0.10452961672473868 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 156 0.10452961672473868 No Hit GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA 154 0.10318949343339587 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 153 0.10251943178772448 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 153 0.10251943178772448 No Hit CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT 152 0.10184937014205307 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 150 0.10050924685071026 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 150 0.10050924685071026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.350308228357009E-4 14-15 0.0 0.0 0.0 0.0010050924685071027 6.700616456714018E-4 16-17 0.0 0.0 0.0 0.0013401232913428035 6.700616456714018E-4 18-19 0.0 0.0 0.0 0.0013401232913428035 6.700616456714018E-4 20-21 0.0 0.0 0.0 0.0016751541141785044 6.700616456714018E-4 22-23 0.0 0.0 0.0 0.012396140444920933 6.700616456714018E-4 24-25 0.0 0.0 0.0 0.03618332886625569 6.700616456714018E-4 26-27 0.0 0.0 0.0 0.07236665773251139 6.700616456714018E-4 28-29 0.0 0.0 0.0 0.14640846957920128 6.700616456714018E-4 30-31 0.0 0.0 0.0 0.29248190833556686 6.700616456714018E-4 32-33 0.0 0.0 0.0 0.5002010184937015 6.700616456714018E-4 34-35 0.0 0.0 0.0 0.8422674886089521 6.700616456714018E-4 36-37 0.0 0.0 0.0 1.2952291610828195 6.700616456714018E-4 38-39 0.0 0.0 0.0 1.8041409809702493 6.700616456714018E-4 40-41 0.0 0.0 0.0 2.277204502814259 6.700616456714018E-4 42-43 0.0 0.0 0.0 2.6980032162958993 6.700616456714018E-4 44-45 0.0 0.0 0.0 3.156660412757974 6.700616456714018E-4 46-47 0.0 0.0 0.0 3.674953095684803 6.700616456714018E-4 48-49 0.0 0.0 0.0 4.220383275261324 6.700616456714018E-4 50-51 0.0 0.0 0.0 4.7694987938890385 6.700616456714018E-4 52-53 0.0 0.0 0.0 5.222795497185741 6.700616456714018E-4 54-55 0.0 0.0 0.0 5.751139104797641 6.700616456714018E-4 56-57 0.0 0.0 0.0 6.3923880997051725 6.700616456714018E-4 58-59 0.0 0.0 0.0 6.954904851246314 6.700616456714018E-4 60-61 0.0 0.0 0.0 7.583757705708925 6.700616456714018E-4 62-63 0.0 0.0 0.0 8.147279549718574 6.700616456714018E-4 64-65 0.0 0.0 0.0 8.696730099169123 6.700616456714018E-4 66-67 0.0 0.0 0.0 9.370142053068882 6.700616456714018E-4 68-69 0.0 0.0 0.0 10.11625569552399 6.700616456714018E-4 70-71 0.0 0.0 0.0 10.891181988742964 6.700616456714018E-4 72-73 0.0 0.0 0.0 11.646341463414634 6.700616456714018E-4 74-75 0.0 0.0 0.0 12.397815599035113 6.700616456714018E-4 76-77 0.0 0.0 0.0 13.223331546502278 6.700616456714018E-4 78-79 0.0 0.0 0.0 14.009983918520504 6.700616456714018E-4 80-81 0.0 0.0 0.0 14.899825783972126 6.700616456714018E-4 82-83 0.0 0.0 0.0 15.764875368533906 6.700616456714018E-4 84-85 0.0 0.0 0.0 16.59977217904047 6.700616456714018E-4 86-87 0.0 0.0 0.0 17.467502010184937 6.700616456714018E-4 88-89 0.0 0.0 0.0 18.347292950951488 6.700616456714018E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTT 20 0.0020707329 71.248245 5 CATGGAT 25 0.005013228 56.998596 4 GGGAATG 25 0.005013228 56.998596 5 GGTATCA 1465 0.0 47.059803 1 CTCTAGG 45 9.551859E-4 42.263668 1 ACATGGA 45 9.599292E-4 42.22118 3 ATGGGGC 55 0.002572779 34.5446 5 AGGTTAG 55 0.002572779 34.5446 5 TGGGGTC 55 0.002572779 34.5446 8 TGTTACC 30 0.0039467835 31.665882 74-75 TGGGGGA 60 0.003940514 31.665882 6 GGTTAGA 60 0.003940514 31.665882 6 GTATCAA 2225 0.0 31.62652 1 CATGGGG 500 0.0 28.499296 4 TCGCCGC 35 0.008355424 27.16039 94-95 CATCTGC 35 0.008355424 27.16039 94-95 GGTACCT 35 0.008382803 27.142185 50-51 GTTAGAG 70 0.008369605 27.142185 7 TAGCCCA 70 0.008369605 27.142185 9 ATGGGGT 235 5.456968E-12 26.275946 5 >>END_MODULE