FastQCFastQC Report
Thu 26 May 2016
SRR938362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149240
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25751.7254087376038594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25431.7039667649423746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16741.1216831948539265No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14830.9937014205306889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12990.8704100777271508No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT9480.6352184400964889TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.4616724738675958No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6740.4516215491825248No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6160.41275797373358347No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6120.4100777271508979No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT5780.38729563119807026RNA PCR Primer, Index 1 (95% over 23bp)
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5760.3859555079067274No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5120.3430715625837577No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5040.33771106941838647No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA4930.33034039131600107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.31492897346555887No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4640.31090860359153044No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4500.30152774055213083No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA4020.2693647815599035No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3920.2626641651031895No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3640.24390243902439024No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT3600.24122219244170465No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA3580.23988206915036187No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA3540.23720182256767625No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT3520.23586169927633344No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA3490.2338515143393192No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT3240.21709997319753419No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT3060.20503886357544895No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT3040.20369874028410614No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.20101849370142053No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC2980.19967837041007774No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC2970.1990083087644063No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT2880.1929777539533637No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT2830.1896274457250067No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2830.1896274457250067No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2830.1896274457250067No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2790.1869471991423211No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.1856070758509783No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2680.17957652103993568No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC2610.17488608952023588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2570.17220584293755026No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG2440.16349504154382202No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT2380.15947467166979362No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA2350.15746448673277944No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2340.156794425087108No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA2320.15545430179576522No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2280.1527740552130796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2270.15210399356740822No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG2270.15210399356740822No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2270.15210399356740822No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC2240.150093808630394No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA2150.1440632538193514No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA2130.14272313052800858No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC2110.14138300723666578No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA2090.140042883945323No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT2090.140042883945323No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC2080.13937282229965156No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA2010.13468239077995176No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA1980.13267220584293754No Hit
GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT1980.13267220584293754No Hit
AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT1940.12999195926025195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.12999195926025195No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA1870.12530152774055214No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1860.12463146609488072No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT1860.12463146609488072No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG1840.12329134280353793TruSeq Adapter, Index 1 (95% over 24bp)
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC1820.12195121951219512No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA1780.11927097292950951No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.11726078799249531No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC1750.11726078799249531No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC1730.11592066470115252No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT1720.1152506030554811TruSeq Adapter, Index 1 (96% over 26bp)
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA1710.11458054140980971No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG1710.11458054140980971No Hit
GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG1700.1139104797641383No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA1700.1139104797641383No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC1680.1125703564727955No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA1650.11056017153578131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1630.1092200482444385No Hit
GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA1600.10720986330742428No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC1600.10720986330742428No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC1590.10653980166175289No Hit
GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC1590.10653980166175289No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT1560.10452961672473868No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT1560.10452961672473868No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC1560.10452961672473868No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT1560.10452961672473868No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC1550.10385955507906729No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC1540.10318949343339587No Hit
CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA1530.10251943178772448No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT1520.10184937014205307No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.10050924685071026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA301.2936168E-463.3720131
GGTATCA16700.040.9830971
GGGTGAG853.6140955E-739.11538
AGGTACA500.001611786537.9977238
GGTACAA500.001611786537.9977239
AAGGTAC500.001611786537.9977237
CATAGGG801.0668637E-535.6467552
CATGGGT1900.035.0213744
GGACCTA550.002564771634.566555
GATGTGG550.002573223834.5433859
GGATGTG550.002573223834.5433858
CCATAGG851.6159676E-533.549891
GACCTAA851.6234073E-533.52746
GGTGAGA1001.2897781E-633.2480059
TTAGGCA1753.6379788E-1232.591324
ATGGGTA1509.349606E-1031.6860035
CTTTAGG1805.456968E-1231.6860032
GGTAATC600.00394119331.664778
TGGATAT600.00394119331.664779
GTATCAA24850.031.5584961
Produced by FastQC (version 0.11.5)