##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149240 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.648927901366925 31.0 31.0 34.0 30.0 34.0 2 31.875850978290003 31.0 31.0 34.0 30.0 34.0 3 31.831439292414903 31.0 31.0 34.0 30.0 34.0 4 35.5996448673278 37.0 35.0 37.0 33.0 37.0 5 35.50022782095953 37.0 35.0 37.0 33.0 37.0 6 35.57839721254356 37.0 35.0 37.0 33.0 37.0 7 35.52922138836773 37.0 35.0 37.0 33.0 37.0 8 35.54551728759046 37.0 35.0 37.0 33.0 37.0 9 36.99811042615921 39.0 37.0 39.0 33.0 39.0 10-11 36.96117997855802 39.0 37.0 39.0 33.0 39.0 12-13 36.87457786116323 39.0 37.0 39.0 32.0 39.0 14-15 37.754556419190564 39.5 37.0 41.0 32.0 41.0 16-17 37.69656258375771 39.0 36.5 41.0 32.5 41.0 18-19 37.990709595282766 39.5 37.0 41.0 33.0 41.0 20-21 38.13992897346556 40.0 37.0 41.0 33.0 41.0 22-23 37.98563722862503 40.0 37.0 41.0 33.0 41.0 24-25 37.821720048244444 40.0 37.0 41.0 33.0 41.0 26-27 37.58379120879121 40.0 36.5 41.0 32.5 41.0 28-29 37.35943111766282 39.0 36.0 41.0 32.0 41.0 30-31 36.868416644331276 39.0 35.0 40.5 31.5 41.0 32-33 36.36842669525596 38.0 35.0 40.0 31.0 41.0 34-35 35.93302398820691 38.0 35.0 40.0 30.0 41.0 36-37 35.62025261324042 38.0 35.0 40.0 30.0 41.0 38-39 35.2517254087376 38.0 35.0 40.0 28.5 41.0 40-41 34.96771978021978 38.0 35.0 40.0 26.5 41.0 42-43 34.44317207183061 38.0 34.0 40.0 23.5 41.0 44-45 33.707531492897346 38.0 33.5 40.0 17.0 41.0 46-47 32.72005159474672 38.0 33.0 40.0 2.0 41.0 48-49 31.872319753417315 38.0 32.0 40.0 2.0 41.0 50-51 31.249537657464487 37.0 31.0 40.0 2.0 41.0 52-53 30.71843674618065 37.0 30.0 40.0 2.0 41.0 54-55 30.282605199678372 37.0 29.0 40.0 2.0 41.0 56-57 29.807595148753684 36.0 28.0 40.0 2.0 41.0 58-59 29.22675556151166 36.0 26.0 39.5 2.0 41.0 60-61 28.72004489413026 35.5 25.0 39.0 2.0 40.5 62-63 28.110640578933264 35.0 22.5 39.0 2.0 40.0 64-65 27.472179040471723 35.0 19.0 38.5 2.0 40.0 66-67 26.818771777003484 34.0 10.5 38.0 2.0 40.0 68-69 26.150033503082284 34.0 4.5 37.0 2.0 39.0 70-71 25.600053604931652 33.5 2.0 37.0 2.0 39.0 72-73 25.212211873492365 33.0 2.0 36.0 2.0 39.0 74-75 24.772554274993297 33.0 2.0 36.0 2.0 38.0 76-77 23.6173914500134 30.5 2.0 34.5 2.0 36.0 78-79 24.141242294291075 32.0 2.0 35.0 2.0 37.0 80-81 23.88343942642723 32.0 2.0 35.0 2.0 36.0 82-83 23.513324175824174 32.0 2.0 35.0 2.0 36.0 84-85 23.1616255695524 31.5 2.0 35.0 2.0 35.5 86-87 22.738732913428038 31.0 2.0 34.5 2.0 35.0 88-89 22.249725274725275 31.0 2.0 34.0 2.0 35.0 90-91 21.863324175824175 30.5 2.0 34.0 2.0 35.0 92-93 21.386407799517556 30.0 2.0 34.0 2.0 35.0 94-95 20.82202157598499 29.0 2.0 34.0 2.0 35.0 96-97 20.06555883141249 29.0 2.0 34.0 2.0 35.0 98-99 19.17053738943983 27.5 2.0 34.0 2.0 35.0 100-101 16.336159876708656 13.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5088699499836302 1101 2 0.5351288647826884 1101 3 0.4543022538379269 1101 4 0.4165793251618055 1101 5 0.29243505685356297 1101 6 0.3857718210402652 1101 7 0.42883791493936485 1101 8 0.4047505697668896 1101 9 0.16864358630470377 1101 10-11 0.06645737759014736 1101 12-13 0.3046053248127407 1101 14-15 0.6156904966813741 1101 16-17 0.7101179160866522 1101 18-19 0.6354853263819749 1101 20-21 0.36628079866206065 1101 22-23 0.8083055313900758 1101 24-25 1.3133867718217118 1101 26-27 1.058488864404076 1101 28-29 0.7973599907515379 1101 30-31 0.7045594259081938 1101 32-33 0.9511025290678461 1101 34-35 1.065204718838494 1101 36-37 1.1139918739011634 1101 38-39 1.6716136669126627 1101 40-41 1.132358202986623 1101 42-43 0.9607178056483789 1101 44-45 1.2082223038135211 1101 46-47 1.9810112690027069 1101 48-49 1.4212556185507133 1101 50-51 1.21762185480231 1101 52-53 1.9719057589403661 1101 54-55 1.1550718364557078 1101 56-57 0.6802556865118596 1101 58-59 1.1877964454675656 1101 60-61 0.9426274657196281 1101 62-63 1.215313677991773 1101 64-65 0.552856509415264 1101 66-67 0.3975786952125979 1101 68-69 0.577856071863522 1101 70-71 -0.6618291174231743 1101 72-73 -0.320657920523594 1101 74-75 -0.24222060164246173 1101 76-77 -0.10954948856018021 1101 78-79 -0.3584852000964709 1101 80-81 0.058986075272787986 1101 82-83 0.11444055263584119 1101 84-85 -0.3837380478793939 1101 86-87 -0.3189214055458187 1101 88-89 -0.3434769260029036 1101 90-91 -0.0372381160889077 1101 92-93 0.4763514133674924 1101 94-95 0.4991184564974809 1101 96-97 1.0409352763275805 1101 98-99 0.6860188204184432 1101 100-101 0.48886189934705726 1102 1 0.4848256843900707 1102 2 0.4021308768551215 1102 3 0.6892117105783733 1102 4 0.0992755022241738 1102 5 -0.02205186467561049 1102 6 0.40005753532597765 1102 7 0.46404918254499705 1102 8 0.6093783363664897 1102 9 0.7928890591417215 1102 10-11 0.5163064721575523 1102 12-13 0.6040017030823535 1102 14-15 0.6616663518121655 1102 16-17 0.5238503104528505 1102 18-19 0.49574689579847586 1102 20-21 0.4502848228069283 1102 22-23 0.5957300786737818 1102 24-25 0.7860225867110486 1102 26-27 0.5766981199372765 1102 28-29 0.6401165299869476 1102 30-31 0.17850308788003133 1102 32-33 0.11151500391694924 1102 34-35 0.2800940548545867 1102 36-37 0.685118634464537 1102 38-39 1.0116539083613532 1102 40-41 0.512338082262275 1102 42-43 0.2306373227509937 1102 44-45 0.9427293460670398 1102 46-47 0.8446430597471775 1102 48-49 0.005662057182508562 1102 50-51 -0.182830861495475 1102 52-53 -0.7347843819047029 1102 54-55 -0.2065981836650188 1102 56-57 -0.5692915971903538 1102 58-59 -0.16617739759078987 1102 60-61 -0.18579305741920038 1102 62-63 -0.4149680121490711 1102 64-65 -0.5487028467215573 1102 66-67 -0.26690822631657696 1102 68-69 -0.773049360732049 1102 70-71 -1.1598673467994338 1102 72-73 -1.0996317635819466 1102 74-75 -0.9669187907772709 1102 76-77 -1.171823130411287 1102 78-79 -1.5018453610622693 1102 80-81 -1.826426399576306 1102 82-83 -1.8603582401207 1102 84-85 -2.283285331581606 1102 86-87 -2.076366073553867 1102 88-89 -1.6533360809324797 1102 90-91 -1.4947330859078178 1102 92-93 -0.9181656892482017 1102 94-95 -1.0353382839451726 1102 96-97 -0.6977971180386184 1102 98-99 -1.1344037147928212 1102 100-101 -1.7150113400895641 1103 1 0.21268599695036272 1103 2 0.32514843425431295 1103 3 0.030133956381959592 1103 4 0.29896680069996506 1103 5 0.43646636800816196 1103 6 0.13978356272323822 1103 7 0.36719407932292825 1103 8 0.37167816272188503 1103 9 0.49692147280176613 1103 10-11 0.3482088453005261 1103 12-13 0.294624894284361 1103 14-15 0.6436748411040796 1103 16-17 0.807035724305841 1103 18-19 0.5325499056383336 1103 20-21 0.06686788281079004 1103 22-23 0.08531140223156797 1103 24-25 0.329433738553611 1103 26-27 0.262304911370812 1103 28-29 0.3351290709863761 1103 30-31 0.36825805604518536 1103 32-33 0.5664156406138403 1103 34-35 -0.13655653360768838 1103 36-37 -0.25724100281117046 1103 38-39 -0.43210453269595206 1103 40-41 -0.42674160131866756 1103 42-43 -0.25855812390152977 1103 44-45 0.217811344909407 1103 46-47 0.3502382748735542 1103 48-49 0.9278113915448465 1103 50-51 0.7683557099882208 1103 52-53 0.04348110140612249 1103 54-55 0.32239081884317855 1103 56-57 0.7877410094081405 1103 58-59 0.607023451338403 1103 60-61 -0.1341337867265544 1103 62-63 0.07490271908766388 1103 64-65 0.530498388084542 1103 66-67 0.27722644472335745 1103 68-69 -0.4631419711893159 1103 70-71 -0.1835023072470534 1103 72-73 0.35850059219656316 1103 74-75 0.08845454512857387 1103 76-77 -0.14399175861888835 1103 78-79 0.32595706996223583 1103 80-81 0.037166114411732565 1103 82-83 0.06126051349690087 1103 84-85 0.0018294667977123424 1103 86-87 0.10113730286905565 1103 88-89 0.40309841646284994 1103 90-91 0.2477423144394706 1103 92-93 0.6711165797080021 1103 94-95 0.43967618643877415 1103 96-97 0.8820311667385354 1103 98-99 0.29218320398008757 1103 100-101 0.2535879267443164 1104 1 0.02147231577071196 1104 2 0.3725264989956081 1104 3 -0.21744478892822272 1104 4 0.09501426601712382 1104 5 0.26641139898276833 1104 6 0.030630783733620603 1104 7 0.3587742206718474 1104 8 0.43332199833832163 1104 9 0.6079065526377292 1104 10-11 0.46434181799051544 1104 12-13 0.23053799087279003 1104 14-15 0.833797867018049 1104 16-17 0.6204682345579968 1104 18-19 0.6360312772463956 1104 20-21 0.57578944288408 1104 22-23 0.8730917006348449 1104 24-25 0.4963258073085157 1104 26-27 0.65070906458606 1104 28-29 0.7827558051282466 1104 30-31 0.46124732399737667 1104 32-33 0.23051563188860058 1104 34-35 0.21884439126797872 1104 36-37 0.13692181020689276 1104 38-39 -0.06451008194994756 1104 40-41 0.21920988633512195 1104 42-43 0.5007996982780369 1104 44-45 0.8677764439409117 1104 46-47 0.913518093388511 1104 48-49 0.16411276140786057 1104 50-51 -0.2622917029774925 1104 52-53 -0.078890419403578 1104 54-55 -0.45821296791190136 1104 56-57 -0.8417415837338176 1104 58-59 -0.8726903607198793 1104 60-61 -1.070134135736236 1104 62-63 -0.5410557487598204 1104 64-65 -0.8473482221588959 1104 66-67 -0.7778080199830377 1104 68-69 -0.4049009800018091 1104 70-71 -0.5575752502712206 1104 72-73 -0.306144726711036 1104 74-75 0.17363472137846614 1104 76-77 0.32252512472462413 1104 78-79 -0.6485215968207676 1104 80-81 -0.6651722504778945 1104 82-83 -0.8494584464542392 1104 84-85 -0.19586270666010108 1104 86-87 0.08403582830313638 1104 88-89 0.30461224597161873 1104 90-91 -0.24071855284959298 1104 92-93 0.05321674548759958 1104 94-95 0.3854924328396123 1104 96-97 0.7629097986205693 1104 98-99 0.6876111771063407 1104 100-101 0.09188737705406425 1105 1 0.3017270928407747 1105 2 0.366781525847113 1105 3 0.6296057085531466 1105 4 0.44683142600214154 1105 5 0.5030793145566435 1105 6 0.4065934830383995 1105 7 0.25890327441648964 1105 8 0.4725376846128313 1105 9 0.5064120270143206 1105 10-11 0.714893418673249 1105 12-13 0.48004883555036315 1105 14-15 0.8031788253461798 1105 16-17 0.761261706936331 1105 18-19 0.640527343188694 1105 20-21 0.533180892032739 1105 22-23 0.5340758395133207 1105 24-25 0.4211832237358095 1105 26-27 0.7892545039932486 1105 28-29 0.7637932307141924 1105 30-31 0.8365855696990465 1105 32-33 1.1001921649221984 1105 34-35 0.9743083602950833 1105 36-37 0.9074559261887387 1105 38-39 1.239307411076993 1105 40-41 1.6240015269828874 1105 42-43 2.1642192062085996 1105 44-45 2.057568709042272 1105 46-47 2.0210906340820713 1105 48-49 2.389602957486286 1105 50-51 1.9913613506006271 1105 52-53 2.1727227524044253 1105 54-55 1.8833631529823158 1105 56-57 1.8169737070534104 1105 58-59 1.9592483595665335 1105 60-61 1.7692147673069307 1105 62-63 2.1940718479264163 1105 64-65 1.784952681217316 1105 66-67 1.549072626118292 1105 68-69 1.2436357170549321 1105 70-71 1.9578487537252833 1105 72-73 1.7480162157976018 1105 74-75 1.5983256168449351 1105 76-77 1.3448389334753017 1105 78-79 1.2439891229660844 1105 80-81 1.2633278119674678 1105 82-83 1.634118423784301 1105 84-85 1.7271629791981091 1105 86-87 1.791396054771642 1105 88-89 1.419594969421933 1105 90-91 1.5051288306898059 1105 92-93 1.428055428306802 1105 94-95 1.20138269552643 1105 96-97 1.3850529233864002 1105 98-99 0.5373260099609247 1105 100-101 0.3945808535954214 1106 1 0.1758992782712383 1106 2 0.2445896027202501 1106 3 0.29181407976035345 1106 4 0.1959549164579215 1106 5 -0.36395094125428784 1106 6 0.0027065419484912923 1106 7 0.0513544712307521 1106 8 0.25451405131845206 1106 9 0.41391321733592434 1106 10-11 -0.033992007461883134 1106 12-13 0.17101970513439824 1106 14-15 0.1928901182518601 1106 16-17 0.3178993730402979 1106 18-19 0.5104143708474425 1106 20-21 0.429904261292144 1106 22-23 0.3528656070759837 1106 24-25 0.32251638393145043 1106 26-27 0.2420744840824156 1106 28-29 0.43439877977709074 1106 30-31 0.6144932007426362 1106 32-33 0.31224254798932094 1106 34-35 -0.07665904653519817 1106 36-37 -0.5429617684829466 1106 38-39 -0.26168813062754737 1106 40-41 -0.5546332823492364 1106 42-43 -0.45446667874140445 1106 44-45 -0.1616452458553539 1106 46-47 -0.7276216543653149 1106 48-49 -0.14714551673783305 1106 50-51 0.49077701090448755 1106 52-53 0.061806421192024175 1106 54-55 -0.23707575105612122 1106 56-57 0.030279338356702823 1106 58-59 -0.12149872955608743 1106 60-61 -0.19178596852824015 1106 62-63 0.07458520117058143 1106 64-65 -0.8979240014645846 1106 66-67 0.3150337567073933 1106 68-69 -0.03344950997186302 1106 70-71 -0.10532486009110187 1106 72-73 -0.41919150614327094 1106 74-75 -0.6030767984258105 1106 76-77 -0.4846204440947126 1106 78-79 -0.09997053595266792 1106 80-81 -0.5900678509334547 1106 82-83 -0.4423002231446702 1106 84-85 -0.28967466093520855 1106 86-87 0.2733680930350708 1106 88-89 0.46281446472557164 1106 90-91 0.5584335963046634 1106 92-93 0.7487497104346623 1106 94-95 0.6536832625523523 1106 96-97 -0.29979320049361746 1106 98-99 -0.24278912660142637 1106 100-101 -0.6069990278052622 1107 1 0.37179078710829216 1107 2 0.15596598944146578 1107 3 0.3048482047023491 1107 4 0.18418624053941812 1107 5 0.13902286395092034 1107 6 0.113433331504325 1107 7 0.2887105264043299 1107 8 0.3250417435612505 1107 9 0.639753686395693 1107 10-11 0.19364118104783756 1107 12-13 0.41525136666896856 1107 14-15 0.22551761224097788 1107 16-17 0.5312962600357025 1107 18-19 0.32074465304257416 1107 20-21 0.3496747932025528 1107 22-23 0.06417450318261331 1107 24-25 0.12371434234036371 1107 26-27 0.30039059324848694 1107 28-29 0.5407175885677376 1107 30-31 0.1523301265451451 1107 32-33 0.24962391214337032 1107 34-35 0.33030935942084483 1107 36-37 0.9649472879139012 1107 38-39 1.129757433973623 1107 40-41 1.4357703958892643 1107 42-43 1.8129016091060635 1107 44-45 1.947394278335814 1107 46-47 2.0982314691846895 1107 48-49 2.1418197677772888 1107 50-51 2.2122305900161408 1107 52-53 1.9338484340996018 1107 54-55 2.0895577327250443 1107 56-57 2.3875577793235045 1107 58-59 2.2578828876877637 1107 60-61 2.4012034438815952 1107 62-63 2.9207276907306294 1107 64-65 2.9456454084143537 1107 66-67 2.607542298488301 1107 68-69 2.1587932875141114 1107 70-71 2.3309597815759133 1107 72-73 2.3212438083208085 1107 74-75 1.740513702270185 1107 76-77 1.8034168444698473 1107 78-79 2.341924263051844 1107 80-81 2.2711334820061815 1107 82-83 2.2078663943100914 1107 84-85 2.628978057671109 1107 86-87 2.010787420659824 1107 88-89 2.556204602659516 1107 90-91 2.4312559694433986 1107 92-93 1.9162740703283667 1107 94-95 1.5924988022663626 1107 96-97 1.5272410088116537 1107 98-99 1.1239169095904167 1107 100-101 1.133288650939413 1108 1 0.04848033959402187 1108 2 0.040323669977492926 1108 3 -0.4190743695386949 1108 4 -0.18991418133170157 1108 5 0.002824667243174872 1108 6 -0.2557866205181796 1108 7 -0.18155169545024563 1108 8 0.23851680353312332 1108 9 0.08098124864236667 1108 10-11 0.0382106243433995 1108 12-13 0.12343649364390785 1108 14-15 0.36698919798008234 1108 16-17 -0.196700265731522 1108 18-19 -0.06841077751413138 1108 20-21 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-0.49030415904798375 2316 58-59 -0.4511031684320521 2316 60-61 -0.04009454200237528 2316 62-63 -0.5245511868730901 2316 64-65 -0.8166415600828039 2316 66-67 -1.3654521928182923 2316 68-69 -1.153997209989761 2316 70-71 -0.9853812409753004 2316 72-73 -0.6278007776664509 2316 74-75 -0.8309271653490278 2316 76-77 -0.9397618437725335 2316 78-79 -0.8005701421814173 2316 80-81 -1.0721915309048207 2316 82-83 -1.2787061655108793 2316 84-85 -1.1840276231689657 2316 86-87 -1.445465807090219 2316 88-89 -1.7015850341110088 2316 90-91 -1.56667827052906 2316 92-93 -1.1289574669413902 2316 94-95 -0.6736614662824394 2316 96-97 -0.9293349939426925 2316 98-99 -0.5166838822842585 2316 100-101 0.2334564939416488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 16.0 6 38.0 7 137.0 8 223.0 9 358.0 10 656.0 11 979.0 12 1374.0 13 1909.0 14 2298.0 15 3188.0 16 4093.0 17 5257.0 18 4625.0 19 2962.0 20 2378.0 21 2404.0 22 2385.0 23 2475.0 24 2503.0 25 2512.0 26 2485.0 27 2558.0 28 2853.0 29 3390.0 30 4028.0 31 4655.0 32 5647.0 33 6942.0 34 9033.0 35 12050.0 36 16359.0 37 24376.0 38 11710.0 39 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.51460734387564 25.605065666041277 16.435272045028142 17.445054945054945 2 15.905205644755807 24.09680555090001 40.90235645091006 19.095632353434116 3 17.204409153348745 28.97041578718129 38.22092672630415 15.604248333165813 4 10.564861967300992 24.379522916108282 39.00294827124095 26.05266684534977 5 7.836101199259518 42.76822364714404 36.997960990529336 12.3977141630671 6 20.456311980702225 44.162422942910744 18.937282229965156 16.44398284642187 7 19.563119807022247 44.05387295631198 19.49678370410078 16.886223532564994 8 24.563789868667918 35.3678638434736 20.61243634414366 19.455909943714822 9 25.43218976145805 17.39413025998392 23.72755293487001 33.446127043688016 10-11 21.870812114714553 30.1417180380595 28.384481372286253 19.602988474939696 12-13 23.82002144197266 31.48887697668186 28.103390511927095 16.587711069418386 14-15 21.260720986330742 30.650294827124096 30.098499061913696 17.990485124631466 16-17 15.500536049316537 35.11994103457518 30.264674350040206 19.11484856606808 18-19 15.056620209059233 30.92602519431788 37.86116322701689 16.156191369606006 20-21 16.236598767086573 29.150361833288663 38.470919324577864 16.142120075046904 22-23 16.47279549718574 30.34340659340659 37.79382203162691 15.389975877780756 24-25 15.87510050924685 32.078531224872684 38.4478021978022 13.598566068078263 26-27 15.652861493719488 31.53992287151171 39.408234856581885 13.398980778186912 28-29 14.69847225944787 31.313655856338784 39.98056821227553 14.007303671937818 30-31 16.236263736263737 30.549115518627712 39.37382739212008 13.840793352988475 32-33 14.652238005896542 30.712610560171537 40.39734655588314 14.23780487804878 34-35 14.081902068660499 32.14431704718315 39.5930361127678 14.180744771388557 36-37 15.838635213867642 30.908372113281 37.60633076363831 15.646661909213044 38-39 13.571548229302778 31.808221931852447 38.55060810131671 16.06962173752806 40-41 13.981445184894811 31.545866042142013 39.39531003427514 15.07737873868803 42-43 14.986933797909408 31.201755561511657 38.57377378718842 15.237536853390512 44-45 15.209729295095148 31.698941302599838 37.81492897346556 15.276400428839453 46-47 16.473800589654246 31.3015947467167 35.53068882337175 16.693915840257304 48-49 15.744103457518092 31.03558027338515 36.149155722326455 17.071160546770304 50-51 14.815398016617529 31.81318681318681 34.98860895202359 18.38280621817207 52-53 16.2828330206379 32.16898954703833 34.69478692039667 16.853390511927095 54-55 16.291543822031628 31.105936746180653 33.70477083891718 18.897748592870546 56-57 16.979697132136156 32.8162690967569 32.83570088448137 17.36833288662557 58-59 16.508643795229162 32.59280353792549 32.749932993835436 18.148619673009918 60-61 15.909943714821765 32.73418654516215 32.82430983650496 18.531559903511123 62-63 15.46770302867864 32.783436076119 32.741892254087375 19.006968641114984 64-65 15.089453229697133 32.84340659340659 32.766684534977216 19.300455641919058 66-67 15.694518895738408 33.527539533637096 31.380326990083084 19.397614580541408 68-69 15.489017542448973 31.34422868170305 32.0665648159316 21.100188959916373 70-71 15.450951487536853 32.06512999195926 32.77506030554811 19.708858214955775 72-73 16.7361297239346 30.00871080139373 33.30976949879389 19.94538997587778 74-75 15.331345483784508 31.074443848834093 32.65277405521308 20.941436612168317 76-77 15.343741624229429 30.841262396140444 33.57343875636559 20.24155722326454 78-79 14.574175824175825 30.776266416510317 33.00288126507639 21.64667649423747 80-81 14.87576724114825 29.411134042724274 33.428931357045215 22.28416735908226 82-83 14.814057893326185 30.22279549718574 32.755293487000806 22.20785312248727 84-85 14.561743844378105 29.85991751181873 32.72757122227918 22.850767421523987 86-87 14.601655761157625 30.537770585614112 31.968050711454637 22.892522941773628 88-89 14.983583489681052 30.40605735727687 31.51567944250871 23.09467971053337 90-91 15.015914497269408 29.001909739672328 32.592555365698395 23.38962039735987 92-93 15.175942026072187 28.31824014419775 33.00902241028682 23.496795419443245 94-95 14.897480568212277 28.616992763334224 32.81660412757974 23.66892254087376 96-97 16.286183328866255 28.1221522380059 31.298244438488343 24.293419994639507 98-99 15.28209595282766 28.48197534173144 30.64392924149022 25.591999463950682 100-101 15.678437416242295 28.427030286786387 31.89459930313589 23.999932993835433 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 769.0 1 456.0 2 161.0 3 188.0 4 150.0 5 131.0 6 217.5 7 371.0 8 512.5 9 585.0 10 502.0 11 374.5 12 356.0 13 379.5 14 421.0 15 467.5 16 546.0 17 619.5 18 682.5 19 803.5 20 946.0 21 1059.0 22 1143.0 23 1281.0 24 1469.5 25 1858.5 26 2874.0 27 4145.0 28 4390.5 29 4593.0 30 5635.0 31 6347.5 32 6441.5 33 6646.0 34 7399.5 35 7983.5 36 8250.0 37 8114.0 38 6499.5 39 5064.0 40 4696.0 41 4585.5 42 4616.0 43 4725.0 44 4942.5 45 4497.5 46 3526.5 47 2753.0 48 2788.0 49 2748.0 50 2449.0 51 2101.0 52 1332.5 53 704.0 54 411.5 55 335.5 56 289.5 57 214.0 58 195.5 59 177.5 60 141.0 61 102.0 62 75.5 63 65.5 64 52.5 65 46.5 66 38.5 67 28.0 68 24.5 69 19.0 70 17.5 71 15.0 72 13.5 73 12.0 74 7.0 75 5.5 76 7.0 77 7.5 78 7.5 79 6.5 80 4.5 81 2.0 82 0.5 83 2.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05025462342535513 3 0.003350308228357009 4 0.0 5 0.09916912355936747 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.005695523988206915 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008710801393728223 36-37 3.350308228357009E-4 38-39 0.003350308228357009 40-41 0.004355400696864111 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0013401232913428035 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.002680246582685607 82-83 0.0 84-85 0.004355400696864111 86-87 0.0023452157598499064 88-89 0.0 90-91 0.003350308228357009 92-93 3.350308228357009E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149240.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.554409005628518 #Duplication Level Percentage of deduplicated Percentage of total 1 79.1393656335729 23.38917180380595 2 8.526991180538236 5.040203698740284 3 3.1604960663840207 2.8021978021978025 4 1.7548234974040404 2.07451085499866 5 1.1426757657514681 1.6885553470919326 6 0.8184641893576983 1.4513535245242561 7 0.5690706690547985 1.177298311444653 8 0.46251161947083225 1.0935406057357278 9 0.3672886389915433 0.9769498793889038 >10 3.124220645249053 19.500134012329134 >50 0.5441313170245086 11.463414634146343 >100 0.35821978370780144 19.102117394800324 >500 0.020404924388419073 3.825381935138033 >1k 0.011336069104677262 6.415170195658 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 1.7254087376038594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2543 1.7039667649423746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1674 1.1216831948539265 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1483 0.9937014205306889 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1299 0.8704100777271508 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 948 0.6352184400964889 TruSeq Adapter, Index 1 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.4616724738675958 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 674 0.4516215491825248 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 616 0.41275797373358347 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 612 0.4100777271508979 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 578 0.38729563119807026 RNA PCR Primer, Index 1 (95% over 23bp) CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 576 0.3859555079067274 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 512 0.3430715625837577 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 504 0.33771106941838647 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 493 0.33034039131600107 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 470 0.31492897346555887 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 464 0.31090860359153044 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 450 0.30152774055213083 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 402 0.2693647815599035 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 392 0.2626641651031895 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 364 0.24390243902439024 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 360 0.24122219244170465 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 358 0.23988206915036187 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 354 0.23720182256767625 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 352 0.23586169927633344 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 349 0.2338515143393192 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 324 0.21709997319753419 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 306 0.20503886357544895 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 304 0.20369874028410614 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.20101849370142053 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 298 0.19967837041007774 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 297 0.1990083087644063 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 288 0.1929777539533637 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 283 0.1896274457250067 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 283 0.1896274457250067 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 283 0.1896274457250067 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 279 0.1869471991423211 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.1856070758509783 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 268 0.17957652103993568 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 261 0.17488608952023588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 257 0.17220584293755026 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 244 0.16349504154382202 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 238 0.15947467166979362 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 235 0.15746448673277944 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 234 0.156794425087108 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 232 0.15545430179576522 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 228 0.1527740552130796 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 227 0.15210399356740822 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 227 0.15210399356740822 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 227 0.15210399356740822 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 224 0.150093808630394 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 215 0.1440632538193514 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 213 0.14272313052800858 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 211 0.14138300723666578 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 209 0.140042883945323 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 209 0.140042883945323 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 208 0.13937282229965156 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 201 0.13468239077995176 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 198 0.13267220584293754 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 198 0.13267220584293754 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 194 0.12999195926025195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.12999195926025195 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 187 0.12530152774055214 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 186 0.12463146609488072 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 186 0.12463146609488072 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 184 0.12329134280353793 TruSeq Adapter, Index 1 (95% over 24bp) GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 182 0.12195121951219512 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 178 0.11927097292950951 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.11726078799249531 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 175 0.11726078799249531 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 173 0.11592066470115252 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 172 0.1152506030554811 TruSeq Adapter, Index 1 (96% over 26bp) CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 171 0.11458054140980971 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 171 0.11458054140980971 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 170 0.1139104797641383 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 170 0.1139104797641383 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 168 0.1125703564727955 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 165 0.11056017153578131 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 163 0.1092200482444385 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 160 0.10720986330742428 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 160 0.10720986330742428 No Hit CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC 159 0.10653980166175289 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 159 0.10653980166175289 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 156 0.10452961672473868 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 156 0.10452961672473868 No Hit ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC 156 0.10452961672473868 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 156 0.10452961672473868 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 155 0.10385955507906729 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 154 0.10318949343339587 No Hit CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA 153 0.10251943178772448 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 152 0.10184937014205307 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.10050924685071026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0010050924685071027 0.0 16-17 0.0 0.0 0.0 0.0013401232913428035 0.0 18-19 0.0 0.0 0.0 0.0013401232913428035 0.0 20-21 0.0 0.0 0.0 0.0016751541141785044 0.0 22-23 0.0 0.0 0.0 0.011726078799249532 0.0 24-25 0.0 0.0 0.0 0.03417314392924149 0.0 26-27 0.0 0.0 0.0 0.07002144197266148 0.0 28-29 0.0 0.0 0.0 0.14439828464218707 0.0 30-31 0.0 0.0 0.0 0.28980166175288125 0.0 32-33 0.0 0.0 0.0 0.5015411417850443 0.0 34-35 0.0 0.0 0.0 0.8466228893058161 0.0 36-37 0.0 0.0 0.0 1.3046100241222192 0.0 38-39 0.0 0.0 0.0 1.8202224604663628 0.0 40-41 0.0 0.0 0.0 2.292950951487537 0.0 42-43 0.0 0.0 0.0 2.7073840793352986 0.0 44-45 0.0 0.0 0.0 3.1626909675690165 0.0 46-47 0.0 0.0 0.0 3.6853390511927095 0.0 48-49 0.0 0.0 0.0 4.233784508174752 0.0 50-51 0.0 0.0 0.0 4.782900026802466 0.0 52-53 0.0 0.0 0.0 5.233851514339319 0.0 54-55 0.0 0.0 0.0 5.761525060305548 0.0 56-57 0.0 0.0 0.0 6.4098097024926295 0.0 58-59 0.0 0.0 0.0 6.97467166979362 0.0 60-61 0.0 0.0 0.0 7.6035245242562315 0.0 62-63 0.0 0.0 0.0 8.17408201554543 0.0 64-65 0.0 0.0 0.0 8.72420262664165 0.0 66-67 0.0 0.0 0.0 9.394264272313052 0.0 68-69 0.0 0.0 0.0 10.144398284642186 0.0 70-71 0.0 0.0 0.0 10.91563923880997 0.0 72-73 0.0 0.0 0.0 11.67548914500134 0.0 74-75 0.0 0.0 0.0 12.431988742964354 0.0 76-77 0.0 0.0 0.0 13.263535245242561 0.0 78-79 0.0 0.0 0.0 14.060573572768696 0.0 80-81 0.0 0.0 0.0 14.962811578665237 0.0 82-83 0.0 0.0 0.0 15.837912087912088 0.0 84-85 0.0 0.0 0.0 16.68319485392656 0.0 86-87 0.0 0.0 0.0 17.56700616456714 0.0 88-89 0.0 0.0 0.0 18.46488876976682 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 30 1.2936168E-4 63.372013 1 GGTATCA 1670 0.0 40.983097 1 GGGTGAG 85 3.6140955E-7 39.1153 8 AGGTACA 50 0.0016117865 37.997723 8 GGTACAA 50 0.0016117865 37.997723 9 AAGGTAC 50 0.0016117865 37.997723 7 CATAGGG 80 1.0668637E-5 35.646755 2 CATGGGT 190 0.0 35.021374 4 GGACCTA 55 0.0025647716 34.56655 5 GATGTGG 55 0.0025732238 34.543385 9 GGATGTG 55 0.0025732238 34.543385 8 CCATAGG 85 1.6159676E-5 33.54989 1 GACCTAA 85 1.6234073E-5 33.5274 6 GGTGAGA 100 1.2897781E-6 33.248005 9 TTAGGCA 175 3.6379788E-12 32.59132 4 ATGGGTA 150 9.349606E-10 31.686003 5 CTTTAGG 180 5.456968E-12 31.686003 2 GGTAATC 60 0.003941193 31.66477 8 TGGATAT 60 0.003941193 31.66477 9 GTATCAA 2485 0.0 31.558496 1 >>END_MODULE