##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938361_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150118 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.63037077499034 31.0 31.0 34.0 27.0 34.0 2 30.746013136332753 31.0 31.0 34.0 27.0 34.0 3 30.67085226288653 31.0 31.0 34.0 27.0 34.0 4 34.33552938355161 37.0 35.0 37.0 32.0 37.0 5 34.39024633954622 37.0 35.0 37.0 32.0 37.0 6 34.465653685767194 37.0 35.0 37.0 32.0 37.0 7 34.43337907512757 37.0 35.0 37.0 32.0 37.0 8 34.41105663544678 37.0 35.0 37.0 31.0 37.0 9 35.78604830866385 39.0 35.0 39.0 30.0 39.0 10-11 35.699010112045194 39.0 35.0 39.0 30.0 39.0 12-13 35.57476451857872 39.0 35.0 39.0 30.0 39.0 14-15 36.41627253227461 39.0 36.0 41.0 30.0 41.0 16-17 36.30645558827056 39.0 36.0 41.0 29.5 41.0 18-19 36.45381633115282 39.0 36.0 41.0 30.0 41.0 20-21 36.565854860842805 39.5 36.0 41.0 30.5 41.0 22-23 36.52628265764265 40.0 36.0 41.0 30.0 41.0 24-25 36.41407093086772 39.0 36.0 41.0 30.0 41.0 26-27 36.18924779173717 39.0 35.5 41.0 30.0 41.0 28-29 35.938321853475266 39.0 35.0 41.0 29.5 41.0 30-31 35.47773418244314 38.5 35.0 40.0 28.0 41.0 32-33 34.94953636472641 38.0 35.0 40.0 26.5 41.0 34-35 34.557491440067146 38.0 35.0 40.0 25.0 41.0 36-37 34.18935770527185 38.0 34.5 40.0 23.5 41.0 38-39 33.72462329634021 37.0 34.0 40.0 20.0 41.0 40-41 33.28752714531235 37.5 33.0 40.0 13.0 41.0 42-43 32.74497728453616 37.0 33.0 40.0 5.0 41.0 44-45 31.981544518312262 37.0 32.5 40.0 2.0 41.0 46-47 30.986327422427692 37.0 30.5 40.0 2.0 41.0 48-49 30.15301629384884 37.0 29.0 40.0 2.0 41.0 50-51 29.21348539149203 36.0 26.0 39.5 2.0 40.5 52-53 29.150798038876083 36.0 26.0 39.5 2.0 40.0 54-55 29.084633421708254 36.0 24.5 40.0 2.0 41.0 56-57 28.75545237746306 36.0 23.0 40.0 2.0 41.0 58-59 28.2903715743615 35.5 21.0 39.5 2.0 41.0 60-61 27.86930947654512 35.0 16.0 39.0 2.0 41.0 62-63 27.29216016733503 35.0 7.5 39.0 2.0 40.0 64-65 26.823485524720553 35.0 2.0 39.0 2.0 40.0 66-67 26.24733876017533 34.0 2.0 38.0 2.0 40.0 68-69 25.62371934078525 34.0 2.0 37.0 2.0 39.5 70-71 25.04824538030083 33.0 2.0 37.0 2.0 39.0 72-73 24.47295460904089 33.0 2.0 36.0 2.0 39.0 74-75 23.868723271026795 32.0 2.0 36.0 2.0 38.0 76-77 23.236657163031747 31.5 2.0 35.0 2.0 37.0 78-79 22.589786034985814 31.0 2.0 35.0 2.0 37.0 80-81 21.935370841604602 30.5 2.0 35.0 2.0 36.0 82-83 21.30861055969304 29.5 2.0 34.5 2.0 36.0 84-85 20.747048988129336 29.0 2.0 34.0 2.0 35.0 86-87 20.123609427250564 29.0 2.0 34.0 2.0 35.0 88-89 19.42227114669793 26.5 2.0 34.0 2.0 35.0 90-91 18.700045963841777 25.5 2.0 34.0 2.0 35.0 92-93 18.026592413967677 24.0 2.0 34.0 2.0 35.0 94-95 17.253933572256493 19.0 2.0 33.0 2.0 35.0 96-97 16.344199229939115 4.5 2.0 33.0 2.0 35.0 98-99 15.290821220639764 2.0 2.0 32.0 2.0 35.0 100-101 12.902240237679692 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1674825327156455 1101 2 1.2398966479061926 1101 3 1.1808622514348635 1101 4 0.7727455565210448 1101 5 0.8991456055493146 1101 6 1.1710774134803543 1101 7 0.7322966183520592 1101 8 0.6966123913720565 1101 9 0.9169773250075579 1101 10-11 0.6570848547485966 1101 12-13 1.0670984491724909 1101 14-15 1.487345112326551 1101 16-17 1.648952306839817 1101 18-19 1.2174746887584362 1101 20-21 1.2989748107183559 1101 22-23 1.0790140637850243 1101 24-25 1.252799439221775 1101 26-27 1.257981996838744 1101 28-29 1.477994500810567 1101 30-31 1.3937889658008942 1101 32-33 1.440095639946776 1101 34-35 1.0913215296786376 1101 36-37 1.5606429665363706 1101 38-39 1.544502767030572 1101 40-41 1.8268581038928886 1101 42-43 2.0631753762965133 1101 44-45 1.4554739281287823 1101 46-47 1.1826873163606955 1101 48-49 1.695646973211847 1101 50-51 1.786352193693741 1101 52-53 1.8916036313371727 1101 54-55 1.919098437921054 1101 56-57 1.9901606919599502 1101 58-59 1.7986955503503133 1101 60-61 1.4618101884468118 1101 62-63 1.0541849300731663 1101 64-65 1.3720674353611741 1101 66-67 1.347320829967206 1101 68-69 1.9091975300184103 1101 70-71 1.6196954771263492 1101 72-73 1.2952994604370893 1101 74-75 0.9063526590947042 1101 76-77 1.5120625590787036 1101 78-79 1.783062592368946 1101 80-81 1.7319441347053868 1101 82-83 1.6510317671425803 1101 84-85 1.6506364096245356 1101 86-87 2.224325421478671 1101 88-89 1.703972108388463 1101 90-91 2.1610902695665537 1101 92-93 2.081482829560567 1101 94-95 1.8521644058884803 1101 96-97 2.112384281415853 1101 98-99 2.9143778638387143 1101 100-101 2.626940211624925 1102 1 0.8541231464079324 1102 2 0.934353153912852 1102 3 0.3580224772273084 1102 4 0.24430439282151895 1102 5 0.4485738875003946 1102 6 0.36763263131216917 1102 7 0.307488397191257 1102 8 0.7455340877101619 1102 9 0.6554344099692031 1102 10-11 0.2859023391891782 1102 12-13 0.2859434339749143 1102 14-15 0.5110098973895134 1102 16-17 0.3642511923514675 1102 18-19 0.7099482739371865 1102 20-21 0.9138973754889435 1102 22-23 0.8250699329411901 1102 24-25 0.47707212702029267 1102 26-27 0.5703282119205184 1102 28-29 0.08734462383850428 1102 30-31 0.1799374677548684 1102 32-33 -0.09883908287851995 1102 34-35 0.3717528511433912 1102 36-37 0.7723957527572409 1102 38-39 1.151175223247094 1102 40-41 1.7104157822833912 1102 42-43 0.8911533760070327 1102 44-45 0.49998109269673563 1102 46-47 1.2441289142764553 1102 48-49 1.439965977409269 1102 50-51 0.6358962666420709 1102 52-53 1.1661730335661495 1102 54-55 1.4726517741491598 1102 56-57 1.6600839804242717 1102 58-59 1.3705583380185722 1102 60-61 1.6182727734808253 1102 62-63 0.8351952065241726 1102 64-65 1.1131298177609423 1102 66-67 1.24079311249724 1102 68-69 1.2478138276012736 1102 70-71 1.6323601174505633 1102 72-73 1.8966889524040909 1102 74-75 1.7666073992886488 1102 76-77 1.94707486187216 1102 78-79 1.978821747094667 1102 80-81 1.7414245226052252 1102 82-83 2.233243375190053 1102 84-85 2.7483350635541903 1102 86-87 2.4851446427842134 1102 88-89 2.987298204784466 1102 90-91 3.225571381160126 1102 92-93 3.3275386987167366 1102 94-95 3.8670001274111314 1102 96-97 4.618608047518041 1102 98-99 5.111294928536934 1102 100-101 4.749389191216762 1103 1 0.8077144644145022 1103 2 1.0118913518280301 1103 3 1.0824739028068215 1103 4 0.8521867289631757 1103 5 0.7549187873052361 1103 6 0.8741774454394431 1103 7 0.7164083838636301 1103 8 0.9160800442050032 1103 9 1.2970165890353158 1103 10-11 1.1186882880682347 1103 12-13 1.192697682154268 1103 14-15 1.4105291510427094 1103 16-17 1.3506141796426263 1103 18-19 1.5140322379526054 1103 20-21 1.53229901288244 1103 22-23 1.3358510267581352 1103 24-25 1.512033334122016 1103 26-27 1.5095685374952765 1103 28-29 1.3875730973498506 1103 30-31 1.32478015422258 1103 32-33 0.8002517829408902 1103 34-35 1.1762414036684987 1103 36-37 1.328300365066248 1103 38-39 1.395641880394038 1103 40-41 1.2439014312909578 1103 42-43 0.6353344412647814 1103 44-45 -3.996964888486332E-4 1103 46-47 -0.2192439259348795 1103 48-49 0.47875887569096065 1103 50-51 0.5575250923834183 1103 52-53 0.8497372203432434 1103 54-55 0.6326308307439525 1103 56-57 0.5260461870976023 1103 58-59 0.3643943130768825 1103 60-61 -0.11327085068025156 1103 62-63 -0.044523009478492526 1103 64-65 -0.1883124212794698 1103 66-67 0.3853978057239509 1103 68-69 0.5260993242931669 1103 70-71 0.5429936554580834 1103 72-73 0.5630938265056642 1103 74-75 1.0701201795256914 1103 76-77 1.1287589020454192 1103 78-79 0.9662795032943912 1103 80-81 0.46161586917098774 1103 82-83 0.7928844813826394 1103 84-85 0.896105066886097 1103 86-87 1.1744920653174127 1103 88-89 1.08663658357451 1103 90-91 1.4875067421958725 1103 92-93 1.6336218059564906 1103 94-95 1.99849139305919 1103 96-97 2.1864837198489475 1103 98-99 2.95517136244621 1103 100-101 3.841111786295061 1104 1 0.28683936635819407 1104 2 -0.1231212865403144 1104 3 0.03924196695743731 1104 4 0.028775241122154682 1104 5 -0.267830275712285 1104 6 -0.1038143614114233 1104 7 0.46420756454871537 1104 8 0.3001992806732332 1104 9 0.8143180794911729 1104 10-11 0.7023847929056757 1104 12-13 0.08982572189976423 1104 14-15 0.5242962564205484 1104 16-17 0.5526319605194701 1104 18-19 0.7493237920361082 1104 20-21 0.5550044953194657 1104 22-23 0.7815496234634409 1104 24-25 0.6306596742248729 1104 26-27 0.6894909640619957 1104 28-29 0.907184358571854 1104 30-31 0.9799052304885905 1104 32-33 0.11913089041059521 1104 34-35 0.5996703237417194 1104 36-37 -0.13849123259782914 1104 38-39 0.10371736195945402 1104 40-41 -0.23003738157402864 1104 42-43 0.41305169045854484 1104 44-45 0.3954862967125763 1104 46-47 1.173874700405225 1104 48-49 1.0602109806329985 1104 50-51 0.9140578213993678 1104 52-53 1.009568999302541 1104 54-55 1.2136253395908128 1104 56-57 1.2837599498449244 1104 58-59 1.1185780488042418 1104 60-61 1.2546364098444585 1104 62-63 1.1777161607472557 1104 64-65 1.2796122714774398 1104 66-67 1.486003575792811 1104 68-69 1.7385110736176692 1104 70-71 1.4995656069964767 1104 72-73 1.3044212909874915 1104 74-75 0.9284615026321141 1104 76-77 1.5273686815276797 1104 78-79 1.7768783004703685 1104 80-81 1.8100208199249295 1104 82-83 2.1086693676373223 1104 84-85 2.2822072197667715 1104 86-87 1.7682338939585698 1104 88-89 2.1475404448139415 1104 90-91 2.0472992986327263 1104 92-93 2.196356051576643 1104 94-95 1.9028755994568165 1104 96-97 2.041883353772068 1104 98-99 2.849597406201111 1104 100-101 3.683371875199443 1105 1 0.22568859659815388 1105 2 -0.05113367150633863 1105 3 -0.2782087942019338 1105 4 0.048728797499549614 1105 5 0.011863540405371964 1105 6 0.14143376794266516 1105 7 -0.12025102322112957 1105 8 0.02377371947945761 1105 9 -0.352878961878055 1105 10-11 -0.0776315483689487 1105 12-13 0.08649296194447942 1105 14-15 0.10727122846837034 1105 16-17 0.08335785713941135 1105 18-19 -0.07806074395769258 1105 20-21 -0.05181583666642808 1105 22-23 -0.2651090159104328 1105 24-25 -0.2985138013062283 1105 26-27 0.11541768634633343 1105 28-29 0.2664343800735267 1105 30-31 0.27415568202381024 1105 32-33 0.6011984916834976 1105 34-35 0.1993090955659227 1105 36-37 0.15178140866819234 1105 38-39 -0.5472889165302703 1105 40-41 -0.6448047334274705 1105 42-43 -0.6884577272760453 1105 44-45 -0.46737600654707734 1105 46-47 -0.6866869234014317 1105 48-49 -1.470794437789639 1105 50-51 -1.6917304300842488 1105 52-53 -1.5188347161872677 1105 54-55 -1.9012151179648775 1105 56-57 -1.5946480659803122 1105 58-59 -1.29350159340785 1105 60-61 -0.9015947761880483 1105 62-63 -0.8426029241413637 1105 64-65 -0.8100067188038054 1105 66-67 -1.2993881847645135 1105 68-69 -1.876651909094985 1105 70-71 -1.2137955351726113 1105 72-73 -0.7887884793745954 1105 74-75 -0.7274041548891681 1105 76-77 -0.3238612144042001 1105 78-79 -0.12688019269209505 1105 80-81 0.40043537226000936 1105 82-83 0.7919066601461395 1105 84-85 1.2509652828956952 1105 86-87 1.116045023226654 1105 88-89 1.3300251786231776 1105 90-91 1.5536207917091822 1105 92-93 1.962525312779018 1105 94-95 1.4720679964637853 1105 96-97 1.0963686036215599 1105 98-99 1.1505133776468863 1105 100-101 2.29464305435142 1106 1 -0.2145489005473138 1106 2 -0.33960135819279813 1106 3 -0.32493179031976993 1106 4 -0.11578364826431908 1106 5 0.04045454291736661 1106 6 -0.16521158487761056 1106 7 -0.07206650620810962 1106 8 -0.09442678291834739 1106 9 -0.2873388842347566 1106 10-11 -0.49311453535226946 1106 12-13 -0.7792069695467276 1106 14-15 -0.6923405550438488 1106 16-17 -0.6789623072816937 1106 18-19 -0.19416031006868195 1106 20-21 -0.22009626598818954 1106 22-23 -0.8331839188563066 1106 24-25 -0.6342753907102008 1106 26-27 -0.5902000345945169 1106 28-29 -0.604951781150497 1106 30-31 -0.2249537036806828 1106 32-33 -0.19896136216441107 1106 34-35 -0.826975444283363 1106 36-37 -0.1419842912633058 1106 38-39 -0.26115052877051426 1106 40-41 -0.7637588325368583 1106 42-43 -1.2420087663260517 1106 44-45 -1.3519386920917214 1106 46-47 -0.1870523036252294 1106 48-49 -0.6021714645080678 1106 50-51 -1.3578185780632381 1106 52-53 -0.8964095049518264 1106 54-55 -1.0037727795314453 1106 56-57 -0.667404403043566 1106 58-59 -1.6308845575549178 1106 60-61 0.06654863554979329 1106 62-63 0.16251239899176895 1106 64-65 -0.41416291884947753 1106 66-67 -0.30904792443110196 1106 68-69 -0.714377417201856 1106 70-71 -0.6911419612972978 1106 72-73 -0.5729857846954474 1106 74-75 -0.39986362052059476 1106 76-77 -0.7561288554181296 1106 78-79 0.056860387349008334 1106 80-81 0.826971111013151 1106 82-83 0.8845762194062452 1106 84-85 0.9018444790592035 1106 86-87 1.5996714219478214 1106 88-89 1.5486824063007276 1106 90-91 1.7911864459681475 1106 92-93 2.2003193296778534 1106 94-95 1.6224904673475464 1106 96-97 1.8293441688190946 1106 98-99 1.8103900618213533 1106 100-101 2.317205283170784 1107 1 0.17886400838283834 1107 2 -0.2913697047887318 1107 3 -0.21317228545681743 1107 4 0.18470264704956207 1107 5 -0.24318825107025788 1107 6 -0.012072769669828176 1107 7 -0.1821460818049303 1107 8 -0.01715003667608528 1107 9 -0.4624051972321084 1107 10-11 -0.30602346550258375 1107 12-13 0.11654899862303836 1107 14-15 -0.007879900755604297 1107 16-17 0.1695567024442184 1107 18-19 0.35908902680134247 1107 20-21 0.2906022309172016 1107 22-23 -0.0174014254876127 1107 24-25 0.23880153236672186 1107 26-27 0.6197690193400547 1107 28-29 0.4915999429874347 1107 30-31 0.25904275962611933 1107 32-33 0.49288213916184986 1107 34-35 0.35374957782096317 1107 36-37 0.5107345416279472 1107 38-39 0.7748795331216201 1107 40-41 1.213893687357043 1107 42-43 1.1697949472017939 1107 44-45 0.9319914582177447 1107 46-47 1.0400918166223398 1107 48-49 0.4317144771364916 1107 50-51 1.1618100691516169 1107 52-53 1.3334534481869902 1107 54-55 1.4234155493810228 1107 56-57 1.173245464330435 1107 58-59 1.6515867068128998 1107 60-61 1.6375035427115954 1107 62-63 1.806212664243759 1107 64-65 1.742357859223027 1107 66-67 2.177645267434503 1107 68-69 2.0703042804108733 1107 70-71 2.120153352584225 1107 72-73 2.2748258863920867 1107 74-75 2.648699597870209 1107 76-77 2.8469657511247526 1107 78-79 2.6538763024683725 1107 80-81 2.868129378033487 1107 82-83 3.126110260078214 1107 84-85 3.13356419612375 1107 86-87 3.1848173105419875 1107 88-89 3.9368344841079796 1107 90-91 4.081681583015008 1107 92-93 3.3600673402879337 1107 94-95 3.311217257798475 1107 96-97 3.9180488549375703 1107 98-99 4.703493510097216 1107 100-101 4.156440473268043 1108 1 0.4383810761821145 1108 2 0.37006650726097945 1108 3 0.43068487987274295 1108 4 0.10215584824509705 1108 5 0.1818550083288315 1108 6 0.49068010118119787 1108 7 0.1805249894551011 1108 8 0.38744332497248735 1108 9 0.3894116975295745 1108 10-11 0.42537499288973635 1108 12-13 0.6819100179717879 1108 14-15 0.4838811500545219 1108 16-17 0.36601336439167653 1108 18-19 0.23279124482871794 1108 20-21 0.21690394556247128 1108 22-23 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2.571996287685785 2316 96-97 2.913047221751224 2316 98-99 2.490117713918872 2316 100-101 2.7929136073526415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3206.0 3 528.0 4 413.0 5 332.0 6 371.0 7 424.0 8 465.0 9 565.0 10 660.0 11 1027.0 12 1438.0 13 2075.0 14 2586.0 15 3445.0 16 4168.0 17 5338.0 18 4301.0 19 2659.0 20 2437.0 21 2446.0 22 2399.0 23 2497.0 24 2606.0 25 2786.0 26 2926.0 27 3365.0 28 3833.0 29 4244.0 30 4825.0 31 5556.0 32 6442.0 33 7849.0 34 9187.0 35 11762.0 36 13897.0 37 17029.0 38 9463.0 39 568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98772542914225 24.34801782719382 16.107952077723358 16.556304665940573 2 16.62068322070721 23.774107987791062 40.591387100643765 19.013821690857956 3 18.147526124973343 28.258290680315636 37.36537641288121 16.228806781829814 4 11.046461253407447 22.86738781250208 40.33751224681583 25.748638687274646 5 8.510865217970936 42.13438208238902 36.52512998266898 12.82962271697107 6 21.428857100958528 43.42696404527336 19.447814320566316 15.696364533201798 7 20.374886721040568 43.71101871101871 20.47217335678874 15.44192121115198 8 25.44147241880239 34.36954406727706 20.721549185025257 19.467434328895287 9 26.737529572490086 17.202359134983837 24.501682716337342 31.558428576188728 10-11 22.262759130799832 29.991537226209275 28.44658123929659 19.299122403694298 12-13 24.210357971053124 31.75227230322254 28.23153503744969 15.805834688274647 14-15 21.703336975932196 30.769077000986165 30.765412191156482 16.76217383192516 16-17 15.6800426432129 34.56065830459914 31.141538820315496 18.61776023187247 18-19 15.110658017917169 30.8972911023824 38.85573979124398 15.136311088456452 20-21 16.627404462896383 29.152436200307214 39.40203719191388 14.818122144882532 22-23 17.01749826749827 29.36057359134282 38.66110933418626 14.960818806972654 24-25 15.83321946683819 31.181867566045344 39.17096304393469 13.813949923181774 26-27 15.523291268315278 30.947945789777098 39.57499558467265 13.953767357234973 28-29 14.917416091616992 31.269929594594142 39.96581356062096 13.84684075316791 30-31 16.08257865030021 30.06037464265009 39.76829731512765 14.08874939192206 32-33 14.770118580762528 30.96060746402428 40.32145748718069 13.947816468032507 34-35 14.709900112614863 31.7707188026841 38.97973598811213 14.539645096588902 36-37 15.969387925089041 30.829971047134197 37.7463409108325 15.454300116944255 38-39 14.247164958756981 32.1818823874312 38.25306824087523 15.317884412936584 40-41 14.573637729488073 31.833339440800323 38.81184226744709 14.781180562264515 42-43 14.966552955599383 31.686410639091733 38.1043121368797 15.242724268429189 44-45 15.672559031906708 31.672212618122103 37.468980977219964 15.186247372751223 46-47 16.456467860569234 31.881595778701634 35.754117364886476 15.90781899584266 48-49 16.063731270694674 31.598811451108933 36.25240674488171 16.08505053331468 50-51 15.453512888752693 32.30465354086795 34.62892583179836 17.612907738580986 52-53 16.777307383560778 32.54236046112697 34.012943798068754 16.6673883572435 54-55 16.801734733644437 31.44837601883958 33.76768292479823 17.98220632271775 56-57 17.265947418734864 32.42611276359748 33.499322166004376 16.808617651663273 58-59 17.35385230338763 31.407348189600615 33.217081376369876 18.021718130641883 60-61 16.303363001652187 30.725363747801527 34.55704045195331 18.414232798592973 62-63 16.631471661914784 30.38383649977351 34.327453436009485 18.65723840230222 64-65 16.433163452250092 30.262149828453417 34.20105925851903 19.103627460777457 66-67 16.655952622831236 31.422347607992567 33.06575980335011 18.855939965826085 68-69 16.399520335764965 29.870090936344557 33.88694580460345 19.843442923287032 70-71 16.40138846322971 30.945940543925804 34.1695870587765 18.483083934067984 72-73 17.55705610169661 29.396009820545192 34.52148160979656 18.525452467961635 74-75 16.309290232747163 30.604569665919378 34.10593710130348 18.98020300002998 76-77 16.638629708959723 30.57571810698903 33.952478189467655 18.833173994583593 78-79 16.233770559511836 30.527002991080053 33.361201228407936 19.878025221000183 80-81 16.508395426066624 29.22361044956582 33.84017880042502 20.427815323942536 82-83 16.552247903988167 30.20888502496561 33.29569339102571 19.943173680020518 84-85 16.366337359189412 29.876493041908432 33.38385083237295 20.373318766529213 86-87 16.75887405946794 30.70689373778016 32.31499955034324 20.21923265240866 88-89 17.490305558146662 30.69606758791626 31.857401756326375 19.9562250976107 90-91 17.806001079474658 29.47465566757511 32.29095173682141 20.428391516128816 92-93 18.258708622177213 28.786672485184432 32.94036130330356 20.01425758933479 94-95 18.31220058234444 29.263474210936614 32.42672387945337 19.997601327265578 96-97 19.815639033620723 28.8088162786669 30.745705795344 20.629838892368376 98-99 19.54315143560514 28.78805544543901 29.875710806930343 21.793082312025504 100-101 20.538974268346767 28.88333894625929 30.64124066781593 19.936446117578015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 834.0 1 491.0 2 181.0 3 214.0 4 172.0 5 151.5 6 226.0 7 388.5 8 533.0 9 595.0 10 502.0 11 376.5 12 400.5 13 438.5 14 497.5 15 588.0 16 666.0 17 741.5 18 864.5 19 980.5 20 1080.5 21 1258.5 22 1411.0 23 1512.5 24 1709.0 25 2171.5 26 3135.0 27 4301.5 28 4574.0 29 4851.0 30 5741.0 31 6337.0 32 6440.0 33 6542.0 34 7254.0 35 7900.5 36 8037.0 37 7809.5 38 6344.5 39 4795.5 40 4323.0 41 4255.5 42 4269.5 43 4448.0 44 4697.5 45 4011.5 46 2939.0 47 2286.0 48 2223.0 49 2191.0 50 1977.5 51 1724.0 52 1337.0 53 1061.5 54 836.0 55 636.5 56 526.0 57 470.0 58 426.5 59 385.5 60 323.0 61 271.5 62 222.0 63 204.5 64 198.0 65 182.0 66 160.0 67 140.5 68 120.5 69 95.0 70 91.0 71 80.5 72 64.0 73 59.0 74 51.0 75 41.5 76 37.5 77 32.5 78 21.5 79 17.5 80 18.0 81 11.0 82 7.0 83 6.0 84 3.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3057594692175489 2 0.042633128605497006 3 0.04662998441226235 4 0.05262526812241036 5 0.06528197817716729 6 0.06394969290824551 7 0.030642561185200975 8 0.03463941699196632 9 0.04196698597103612 10-11 0.03264098908858364 12-13 0.03330713172304454 14-15 0.027977990647357412 16-17 0.023648063523361622 18-19 0.025646491426744293 20-21 0.03896934411596211 22-23 0.030642561185200975 24-25 0.05895362314978883 26-27 0.04829534099841458 28-29 0.03896934411596211 30-31 0.03663784489534899 32-33 0.03430634567473587 34-35 0.03264098908858364 36-37 0.030975632502431422 38-39 0.021316564302748503 40-41 0.018985065082135388 42-43 0.019984279033826723 44-45 0.0056622123929175715 46-47 0.014655137958139596 48-49 0.012656710054756925 50-51 0.00432992712399579 52-53 0.004996069758456681 54-55 0.0056622123929175715 56-57 0.004996069758456681 58-59 0.00865985424799158 60-61 0.009325996882452472 62-63 0.0013322852689217814 64-65 0.00865985424799158 66-67 0.002331499220613118 68-69 0.00865985424799158 70-71 0.015987423227061377 72-73 0.01632049454429182 74-75 0.01298978137198737 76-77 0.01232363873752648 78-79 0.003330713172304454 80-81 0.00432992712399579 82-83 0.007660640296300244 84-85 0.003330713172304454 86-87 0.002331499220613118 88-89 0.021316564302748503 90-91 0.029976418550740083 92-93 0.014988209275370042 94-95 0.023314992206131174 96-97 0.021316564302748503 98-99 0.01632049454429182 100-101 0.11091274863773831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 150118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.01990434191769 #Duplication Level Percentage of deduplicated Percentage of total 1 82.38691991384374 28.027951344941982 2 7.215586449970629 4.909471215976765 3 2.76091638926963 2.817783343769568 4 1.5018601918934795 2.043725602526013 5 0.8204425298609751 1.3955688191955662 6 0.6168004699432152 1.2590095791310836 7 0.47385940865478754 1.128445622776749 8 0.44644605443508906 1.2150441652566648 9 0.32504405717642454 0.9952170958845707 >10 2.6747601331505777 18.98972808057661 >50 0.45819463481495987 11.065295301029856 >100 0.301546896416683 18.707283603565195 >500 0.009790483649892304 1.969117627466393 >1k 0.007832386919913843 5.476358597902983 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 1.8159048215403883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 1.7266417085226287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1659 1.1051306305706177 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1244 0.8286814372693482 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.450978563530023 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 654 0.4356572829374226 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 553 0.3683768768568726 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 541 0.3603831652433419 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 531 0.353721738898733 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 485 0.323079177713532 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 463 0.3084240397553924 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.3057594692175489 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 445 0.2964334723350964 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 442 0.2944350444317137 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 413 0.2751169080323479 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 399 0.2657909111498954 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 378 0.2518019158262167 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.2451404894816078 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 365 0.24314206157822513 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 352 0.23448220733023353 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 347 0.23115149415792907 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 344 0.22915306625454643 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 328 0.21849478410317216 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 322 0.21449792829640685 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 316 0.21050107248964145 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 314 0.20916878722071972 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 294 0.1958459345315019 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 294 0.1958459345315019 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 290 0.19318136399365832 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 284 0.18918450818689297 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 281 0.1871860802835103 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 276 0.18385536711120584 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 269 0.17919236866997962 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 266 0.17719394076659695 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 265 0.17652779813213607 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 263 0.17519551286321428 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.1731970849598316 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 260 0.1731970849598316 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 248 0.16520337334630092 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 242 0.16120651753953555 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 242 0.16120651753953555 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 233 0.15521123382938756 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 233 0.15521123382938756 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.15521123382938756 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 217 0.1445529516780133 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 213 0.14188838114016972 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 211 0.14055609587124795 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 211 0.14055609587124795 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 210 0.13988995323678705 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 206 0.1372253826989435 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 206 0.1372253826989435 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 206 0.1372253826989435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 205 0.1365592400644826 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 204 0.1358930974300217 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 199 0.13256238425771727 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 196 0.13056395635433457 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 194 0.1292316710854128 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 186 0.12390253000972569 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 185 0.1232363873752648 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 185 0.1232363873752648 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 184 0.1225702447408039 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 178 0.11857338893403857 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 175 0.11657496103065587 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 175 0.11657496103065587 No Hit CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT 169 0.11257810522389054 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 169 0.11257810522389054 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 168 0.11191196258942965 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 167 0.11124581995496875 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 164 0.10924739205158608 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 161 0.10724896414820342 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 160 0.10658282151374253 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 160 0.10658282151374253 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 160 0.10658282151374253 No Hit ATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTG 158 0.10525053624482072 No Hit GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA 158 0.10525053624482072 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 158 0.10525053624482072 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 158 0.10525053624482072 No Hit GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC 156 0.10391825097589896 No Hit AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT 154 0.10258596570697717 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 153 0.10191982307251629 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.1012536804380554 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 151 0.1005875378035945 No Hit CTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAAC 151 0.1005875378035945 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0029976418550740082 0.0 22-23 0.0 0.0 0.0 0.012656710054756925 0.0 24-25 0.0 0.0 0.0 0.04096777201934478 0.0 26-27 0.0 0.0 0.0 0.07327568979069798 0.0 28-29 0.0 0.0 0.0 0.1475505935330873 0.0 30-31 0.0 0.0 0.0 0.2941019731144833 0.0 32-33 0.0 0.0 0.0 0.491280192914907 0.0 34-35 0.0 0.0 0.0 0.816690869849052 0.0 36-37 0.0 0.0 0.0 1.2470190117107873 0.0 38-39 0.0 0.0 0.0 1.7646118386868996 0.0 40-41 0.0 0.0 0.0 2.252894389746732 0.0 42-43 0.0 0.0 0.0 2.6828894602912374 0.0 44-45 0.0 0.0 0.0 3.1681743694959965 0.0 46-47 0.0 0.0 0.0 3.6811041980308827 0.0 48-49 0.0 0.0 0.0 4.198363953689764 0.0 50-51 0.0 0.0 0.0 4.722285135693255 0.0 52-53 0.0 0.0 0.0 5.179592054250656 0.0 54-55 0.0 0.0 0.0 5.688525026978777 0.0 56-57 0.0 0.0 0.0 6.299710894096644 0.0 58-59 0.0 0.0 0.0 6.890912482180685 0.0 60-61 0.0 0.0 0.0 7.489774710561026 0.0 62-63 0.0 0.0 0.0 8.027684887888194 0.0 64-65 0.0 0.0 0.0 8.608561265138091 0.0 66-67 0.0 0.0 0.0 9.279366898040209 0.0 68-69 0.0 0.0 0.0 10.002797799064735 0.0 70-71 0.0 0.0 0.0 10.796173676707657 0.0 72-73 0.0 0.0 0.0 11.50095258396728 0.0 74-75 0.0 0.0 0.0 12.25769061671485 0.0 76-77 0.0 0.0 0.0 13.110353188824792 0.0 78-79 0.0 0.0 0.0 13.953689764052278 0.0 80-81 0.0 0.0 0.0 14.844655537643721 0.0 82-83 0.0 0.0 0.0 15.667341691202921 0.0 84-85 0.0 0.0 0.0 16.45139157196339 0.0 86-87 0.0 0.0 0.0 17.30538642934225 0.0 88-89 0.0 0.0 0.0 18.20168134400938 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATATGT 35 2.7754958E-4 54.304 4 TGGGATA 60 1.4835205E-6 47.500168 6 GGTATCA 1620 0.0 43.540302 1 GTACACG 35 0.008381724 40.85055 1 AGGTTAG 50 0.0016086757 38.012802 5 ATGGGGT 215 0.0 35.360744 5 ATCCTAC 55 0.002572469 34.545578 6 GGTTAGA 55 0.002572469 34.545578 6 GGGCGCG 35 2.0396755E-4 33.956978 94-95 GCAGAGG 70 2.0448233E-4 33.928688 9 CAGAGGA 35 2.0496894E-4 33.928688 10-11 CCGTATC 30 0.0039302018 31.693182 94-95 AATCAGC 30 0.0039398386 31.677336 40-41 TATCCTA 60 0.0039336127 31.677336 5 CCTACTA 60 0.00394004 31.666779 8 GTTAGAG 60 0.00394004 31.666779 7 GTATCAA 2410 0.0 31.04755 1 CCATAGG 80 4.3884554E-4 29.78686 1 CATCTGC 40 4.463878E-4 29.712357 94-95 ATGGGAT 145 2.3630491E-8 29.49269 5 >>END_MODULE