FastQCFastQC Report
Thu 26 May 2016
SRR938360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151042
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25511.6889342037314126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24761.6392791409012064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15391.0189218892758307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12910.8547291481839489No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6840.45285417301148023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.4389507554190225No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5670.37539227499635863No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5440.3601647223950954No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5260.3482475073158459No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG5080.33633029223659644No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA4820.31911653712212495No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4550.30124071450325074No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4510.29859244448563976No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4350.2879993644151958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.27939248685796003No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4000.2648270017610995No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3900.25820632671707205No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA3900.25820632671707205No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3790.25092358416864186No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA3580.23702016657618413No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT3470.22973742402775388No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.22444088399253187No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3290.21782020894850437No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT3110.2059029938692549No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC3070.20325472385164392No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG3060.20259265634724113No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT2990.1979581838164219No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT2930.19398577879000545No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT2920.1933237112856027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2900.1919995762767972No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT2880.19067544126799169No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA2800.1853789012327697No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC2790.18471683372836695No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT2630.17412375365792296No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA2570.17015134863150647No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT2560.16948928112710374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2550.16882721362270098No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG2540.16816514611829822No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA2490.16485480859628449No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.16485480859628449No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2470.16353067358747897No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2390.158234133552257No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2380.15757206604785423No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA2370.15690999854345147No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC2330.1542617285258405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.15359966102143774No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA2270.150289323499424No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2220.14697898597741027No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT2170.1436686484553965No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2170.1436686484553965No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG2120.14035831093338277No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC2110.13969624342898002No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2110.13969624342898002No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT2110.13969624342898002No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA2030.13439970339375804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.13108936587174427No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA1980.13108936587174427No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC1920.12711696084532778No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC1910.12645489334092505No Hit
GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT1900.1257928258365223No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA1860.1231445558189113No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA1860.1231445558189113No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC1860.1231445558189113No Hit
GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG1770.11718594827928655No Hit
AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT1770.11718594827928655No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1740.1151997457660783No Hit
GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA1720.11387561075727282No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG1700.1125514757484673No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1690.11188940824406457No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG1670.11056527323525907No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT1640.10857907072205082No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA1630.10791700321764806No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT1620.10725493571324533No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA1620.10725493571324533No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG1620.10725493571324533No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG1590.10526873320003709No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC1590.10526873320003709No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT1580.10460666569563433No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC1560.10328253068682884No Hit
GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC1560.10328253068682884No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT1560.10328253068682884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1540.10195839567802333No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC1530.10129632817362057No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG1530.10129632817362057No Hit
AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA1520.10063426066921784No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT1520.10063426066921784No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTC200.002065853771.2910165
GCATCCG205.357811E-447.54310294-95
TGCTTGG205.379749E-447.50372714-15
GGTATCA14950.045.8853071
GGAGCCT500.001609439138.0092778
GCAGTGC500.001609439138.0092777
GTATCAA24350.036.3886831
GGCAGGC550.002565306134.5653422
GCTGAAT550.002569481334.553899
AGTGCCT550.002569481334.553899
GAGCCTG550.002569481334.553899
GGGCAGG600.003884634731.7585831
GCCGTAT300.0039288931.69540294-95
TCACGTG300.0039288931.69540294-95
TAGAGGA753.0555463E-431.6848964
ATAGGGT753.0555463E-431.6848963
TAGGGTC753.0555463E-431.6848964
AGGAGCC600.00393547331.6743987
CAGTGCC600.00393547331.6743988
TGGATAT600.00393547331.6743989
Produced by FastQC (version 0.11.5)