##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938360_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151042 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.64668105560043 31.0 31.0 34.0 27.0 34.0 2 30.77493677255333 31.0 31.0 34.0 27.0 34.0 3 30.675275751115585 31.0 31.0 34.0 27.0 34.0 4 34.34853881701778 37.0 35.0 37.0 32.0 37.0 5 34.38851445293362 37.0 35.0 37.0 32.0 37.0 6 34.444015571827705 37.0 35.0 37.0 32.0 37.0 7 34.40411276333735 37.0 35.0 37.0 31.0 37.0 8 34.38797155758001 37.0 35.0 37.0 31.0 37.0 9 35.77495001390342 39.0 35.0 39.0 30.0 39.0 10-11 35.6460454707962 39.0 35.0 39.0 30.0 39.0 12-13 35.52310615590366 39.0 35.0 39.0 30.0 39.0 14-15 36.48065107718383 39.0 36.0 41.0 30.0 41.0 16-17 36.40817785781438 39.0 36.0 41.0 30.5 41.0 18-19 36.61545464175528 39.5 36.0 41.0 31.0 41.0 20-21 36.68753062062208 40.0 37.0 41.0 31.0 41.0 22-23 36.51657485997272 40.0 36.0 41.0 30.0 41.0 24-25 36.3506177089816 39.0 36.0 41.0 30.0 41.0 26-27 36.122856556454494 39.0 35.5 41.0 29.5 41.0 28-29 35.82698851974948 39.0 35.0 41.0 29.0 41.0 30-31 35.37037049297547 38.5 35.0 40.0 27.5 41.0 32-33 34.82740893261477 38.0 35.0 40.0 26.5 41.0 34-35 34.41898279948623 38.0 34.5 40.0 24.5 41.0 36-37 33.92263741211054 38.0 33.5 40.0 21.5 41.0 38-39 33.486440857509834 37.0 33.0 40.0 18.0 41.0 40-41 33.094073171700586 37.0 33.0 40.0 12.0 41.0 42-43 32.44993445531706 37.0 33.0 40.0 2.0 41.0 44-45 31.579573893354166 37.0 31.5 40.0 2.0 41.0 46-47 30.560105798387205 36.5 30.0 40.0 2.0 41.0 48-49 29.688166205426306 36.0 27.5 40.0 2.0 41.0 50-51 28.802117953946585 35.5 24.5 39.0 2.0 40.5 52-53 28.752913097019373 35.5 24.0 39.0 2.0 40.0 54-55 28.719564756822606 36.0 23.5 39.0 2.0 41.0 56-57 28.344463791528185 35.5 22.0 39.0 2.0 41.0 58-59 27.902252353649978 35.0 18.5 39.0 2.0 40.5 60-61 27.461163120191735 35.0 10.5 39.0 2.0 40.0 62-63 26.82912037711365 35.0 4.5 38.5 2.0 40.0 64-65 26.231988453542723 34.0 2.0 38.0 2.0 40.0 66-67 25.6306921253691 34.0 2.0 37.5 2.0 39.5 68-69 24.863925265820104 33.0 2.0 37.0 2.0 39.0 70-71 24.26870340699938 32.0 2.0 36.0 2.0 39.0 72-73 23.551512162180057 31.5 2.0 35.5 2.0 38.5 74-75 22.79014115279194 31.0 2.0 35.0 2.0 37.5 76-77 22.043607076177487 30.0 2.0 35.0 2.0 37.0 78-79 21.276820354603355 29.0 2.0 34.0 2.0 36.0 80-81 20.478674805683188 28.5 2.0 34.0 2.0 35.5 82-83 19.689788270812095 26.5 2.0 34.0 2.0 35.0 84-85 18.957157611790098 26.0 2.0 33.5 2.0 35.0 86-87 18.172044861694097 24.5 2.0 33.0 2.0 35.0 88-89 17.257958051402923 20.0 2.0 32.5 2.0 35.0 90-91 16.303468571655564 12.0 2.0 32.0 2.0 34.0 92-93 15.380073092252486 2.0 2.0 31.0 2.0 34.0 94-95 14.25503171303346 2.0 2.0 31.0 2.0 34.0 96-97 12.938248963864355 2.0 2.0 30.0 2.0 34.0 98-99 11.404970140755552 2.0 2.0 29.0 2.0 34.0 100-101 8.148630844400895 2.0 2.0 10.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38352256166764676 1101 2 0.501527450020788 1101 3 0.5748668791734701 1101 4 0.2222692734482763 1101 5 -0.2642969233173744 1101 6 -0.35477915721443765 1101 7 -0.40505372813618123 1101 8 -0.3057424963516979 1101 9 -0.5009302072619093 1101 10-11 0.16650466652643559 1101 12-13 -0.058088240319193574 1101 14-15 0.42954685576746954 1101 16-17 0.22788230195858006 1101 18-19 -0.23680252592101425 1101 20-21 -0.23787819925168208 1101 22-23 -0.16826585710870745 1101 24-25 -0.18465785914231247 1101 26-27 0.05790897847578691 1101 28-29 0.2141171920197209 1101 30-31 0.3122074934416972 1101 32-33 0.26928823537651425 1101 34-35 0.7081090727219177 1101 36-37 0.08237760376860592 1101 38-39 0.3301379817442154 1101 40-41 0.32436143333134027 1101 42-43 0.8293833118039444 1101 44-45 1.3281415287721536 1101 46-47 2.1049934514149307 1101 48-49 1.7436451504546255 1101 50-51 1.229005509835531 1101 52-53 0.9280878690105041 1101 54-55 1.5169253041205835 1101 56-57 1.7260071184618617 1101 58-59 1.3786577804753044 1101 60-61 1.06936822924796 1101 62-63 1.279646481806477 1101 64-65 2.1435439054083183 1101 66-67 2.1508352453111996 1101 68-69 1.5949878000763356 1101 70-71 1.931537651611194 1101 72-73 2.01061290625524 1101 74-75 1.7248594564138457 1101 76-77 2.3694144841091855 1101 78-79 2.259646509442856 1101 80-81 2.7976006220699574 1101 82-83 2.556323122085331 1101 84-85 2.255166542771427 1101 86-87 2.558509318885587 1101 88-89 3.6691294078921963 1101 90-91 4.12958624234669 1101 92-93 4.817835407105635 1101 94-95 5.3550623580673395 1101 96-97 4.793634038019805 1101 98-99 4.796941511819499 1101 100-101 3.3379837012256157 1102 1 0.7327759768958586 1102 2 1.0472756232708171 1102 3 0.8333122884713617 1102 4 0.9667890235277028 1102 5 1.1299633753165352 1102 6 1.3521995571771726 1102 7 1.3498970290384449 1102 8 1.182926358884984 1102 9 1.2457075138013067 1102 10-11 1.5976176602784236 1102 12-13 1.1919647089554033 1102 14-15 1.2712020500913113 1102 16-17 1.2942012683887398 1102 18-19 1.5689265855901553 1102 20-21 1.4625877543647476 1102 22-23 1.3364995776048403 1102 24-25 0.9421291788752697 1102 26-27 0.75093555901163 1102 28-29 0.8166534622728179 1102 30-31 0.8223737541639267 1102 32-33 1.0765264012136484 1102 34-35 1.1561393597069909 1102 36-37 0.5741969408436844 1102 38-39 1.056602029186763 1102 40-41 1.0981621322617698 1102 42-43 1.2184016322529558 1102 44-45 1.3508567675733865 1102 46-47 1.2171400150070077 1102 48-49 1.1071419205488766 1102 50-51 1.229482053306885 1102 52-53 1.300797812884273 1102 54-55 1.6518135811511883 1102 56-57 2.3572948448200073 1102 58-59 2.518867459602699 1102 60-61 2.3274165189863893 1102 62-63 1.462745073355773 1102 64-65 1.922401260062557 1102 66-67 2.4474041323174482 1102 68-69 2.130357915505755 1102 70-71 2.201181832485915 1102 72-73 1.8134827569382814 1102 74-75 1.437318420726033 1102 76-77 1.7070720082011057 1102 78-79 1.6186161165592416 1102 80-81 2.35425634588654 1102 82-83 2.7021983735943884 1102 84-85 2.8371849691189865 1102 86-87 2.8997938682872615 1102 88-89 2.8790203323865278 1102 90-91 2.239455987131187 1102 92-93 2.4950566291748917 1102 94-95 2.5861594670369463 1102 96-97 3.4244187462693016 1102 98-99 3.7072719152929725 1102 100-101 3.249531938014771 1103 1 0.6513349497807859 1103 2 0.6927197164010259 1103 3 0.7273910467352316 1103 4 0.635803108034743 1103 5 0.8746218020675158 1103 6 0.8020414657715946 1103 7 0.8215842954943255 1103 8 0.551758398743182 1103 9 0.9777665345927247 1103 10-11 0.5433396110305537 1103 12-13 0.8248333493693139 1103 14-15 1.26001588548462 1103 16-17 0.8524795092625581 1103 18-19 0.810279464769998 1103 20-21 0.7923760076942514 1103 22-23 0.8014799359453519 1103 24-25 0.8282315071305177 1103 26-27 1.2859791539143828 1103 28-29 1.1822518142896783 1103 30-31 1.2685268632949018 1103 32-33 1.3401797498770591 1103 34-35 0.9470994056972089 1103 36-37 0.9056858744452967 1103 38-39 1.2524437461552438 1103 40-41 1.1023779030771337 1103 42-43 1.1372959505436526 1103 44-45 1.6049048656858638 1103 46-47 2.24176394908768 1103 48-49 2.105800538925802 1103 50-51 1.2297215857395791 1103 52-53 1.139688298656047 1103 54-55 1.3685185730070835 1103 56-57 0.8756669824074379 1103 58-59 0.9740748946894087 1103 60-61 1.202021290472448 1103 62-63 1.4054968219425312 1103 64-65 1.0534973604745552 1103 66-67 1.1905774579857429 1103 68-69 0.6621199160806341 1103 70-71 0.40361069135340344 1103 72-73 1.0091807544471436 1103 74-75 1.1960374012760013 1103 76-77 1.4610721998270506 1103 78-79 2.1558155033562123 1103 80-81 1.8298240377520187 1103 82-83 1.831833326166965 1103 84-85 2.546967473670101 1103 86-87 3.208527220783189 1103 88-89 3.2131680759910104 1103 90-91 3.32113654667895 1103 92-93 2.748913101341225 1103 94-95 3.094231710018647 1103 96-97 4.035130636659263 1103 98-99 3.909365428754695 1103 100-101 2.8800531605883073 1104 1 0.6960256737524944 1104 2 0.7796233520274605 1104 3 0.8821416887138795 1104 4 0.9735514524056015 1104 5 1.1460999297424266 1104 6 1.2530483585949526 1104 7 1.0545887555564946 1104 8 1.004071774424581 1104 9 1.0159248442742097 1104 10-11 1.0899224825897704 1104 12-13 1.3526073728860126 1104 14-15 1.3370307974096534 1104 16-17 1.153939165972922 1104 18-19 1.2752972599675516 1104 20-21 1.4570378989408113 1104 22-23 0.8307631724187559 1104 24-25 0.9279747156722138 1104 26-27 0.8017470504769832 1104 28-29 1.0162383890520346 1104 30-31 1.0631162222172819 1104 32-33 1.0259480969509909 1104 34-35 0.762682592614361 1104 36-37 0.8550709264793568 1104 38-39 1.0441498325633134 1104 40-41 0.9883309597965066 1104 42-43 0.9782654509265925 1104 44-45 0.5264933686367179 1104 46-47 0.32254063062122285 1104 48-49 0.38426159222129286 1104 50-51 -0.36612469979450424 1104 52-53 -0.6656232299288973 1104 54-55 -0.4446093271596041 1104 56-57 -0.050246429314864116 1104 58-59 0.19203227631755837 1104 60-61 0.319604747696399 1104 62-63 0.133951117568067 1104 64-65 0.35013654595006827 1104 66-67 0.298024410276593 1104 68-69 -0.23252800931469153 1104 70-71 0.10424591232921188 1104 72-73 0.21269924793725536 1104 74-75 -0.9285289815685296 1104 76-77 -0.008766066605350176 1104 78-79 0.1434039162047931 1104 80-81 -0.31107338081805835 1104 82-83 -0.09315653732810247 1104 84-85 0.46571775523968384 1104 86-87 -0.1605316610476919 1104 88-89 0.33872558376233286 1104 90-91 0.41569887492150315 1104 92-93 0.23062234132861015 1104 94-95 0.4950065895068647 1104 96-97 0.4237191846612429 1104 98-99 0.8369006812662967 1104 100-101 1.08884263664363 1105 1 0.12272350520295561 1105 2 -0.18189679687094795 1105 3 -0.08600097674082363 1105 4 -0.3707328396776788 1105 5 -0.06988933572093003 1105 6 0.13964317312120045 1105 7 -0.27138622604762475 1105 8 0.18214388627152545 1105 9 0.2924257316048653 1105 10-11 -0.44652300068079853 1105 12-13 0.06366221424860186 1105 14-15 -0.08185419588125598 1105 16-17 0.07816978537299946 1105 18-19 0.10326332498075175 1105 20-21 0.5394081538860576 1105 22-23 0.07358877948276898 1105 24-25 -0.12541661878652377 1105 26-27 -0.24296575738346604 1105 28-29 -0.2628595635948088 1105 30-31 -0.32477712496707056 1105 32-33 0.214862956493036 1105 34-35 -0.41577907903000977 1105 36-37 -1.0303552086546048 1105 38-39 -0.8189848252733256 1105 40-41 -0.7543647940016953 1105 42-43 -0.6830988740528525 1105 44-45 -1.1729396054359142 1105 46-47 -1.1321189374002465 1105 48-49 -0.8479809736792419 1105 50-51 -0.5706652356556496 1105 52-53 -0.29922059823604386 1105 54-55 -0.44634070403706616 1105 56-57 -0.19929633625335796 1105 58-59 0.025495462916701683 1105 60-61 0.05256150655888092 1105 62-63 0.041084390303208096 1105 64-65 0.05636517426673393 1105 66-67 -0.10787525643335627 1105 68-69 -0.33061050942290393 1105 70-71 0.06827655769994223 1105 72-73 0.9134091124570141 1105 74-75 0.9626106972610593 1105 76-77 1.262259341350326 1105 78-79 1.1194676308738885 1105 80-81 1.1686262252937034 1105 82-83 1.2696751295549866 1105 84-85 1.5721108643597113 1105 86-87 1.518384497027018 1105 88-89 1.6441404649465952 1105 90-91 2.0493611698615553 1105 92-93 1.6956918128258192 1105 94-95 2.197339619849245 1105 96-97 2.468237212622979 1105 98-99 2.993265655512065 1105 100-101 1.7594049012845865 1106 1 -0.14566665340825935 1106 2 -0.42634956736343455 1106 3 0.026234150855859895 1106 4 0.08803240739779739 1106 5 -0.34523174444662885 1106 6 -0.7090725339684241 1106 7 -0.2869559358848548 1106 8 -0.30971740176811124 1106 9 -0.27761714754254996 1106 10-11 -0.2045264143604939 1106 12-13 0.138430125659454 1106 14-15 -0.34620622119244615 1106 16-17 -0.2522910613860816 1106 18-19 0.02712161564308957 1106 20-21 -0.24482231112371977 1106 22-23 -0.23002056234312818 1106 24-25 0.4343019607896892 1106 26-27 0.05637648482126423 1106 28-29 -0.032207217730764626 1106 30-31 -0.389387257767531 1106 32-33 0.1419475907713732 1106 34-35 -0.3100174537854272 1106 36-37 -0.023340513179810785 1106 38-39 -0.18628843461515743 1106 40-41 0.15140515729091675 1106 42-43 -0.09540477305788642 1106 44-45 -0.09477882944823435 1106 46-47 0.16847221201068763 1106 48-49 -0.5908934252840865 1106 50-51 -1.9927750562293127 1106 52-53 -1.5593887844064795 1106 54-55 -1.7012634799414847 1106 56-57 -1.7280420171159392 1106 58-59 -1.5942122927970495 1106 60-61 -0.9929611611069085 1106 62-63 -1.3190796328432022 1106 64-65 -1.4577682922833652 1106 66-67 -1.181644042079249 1106 68-69 -1.0797883121788274 1106 70-71 -0.9021867868735569 1106 72-73 -0.8565629952820473 1106 74-75 -0.5811843154361611 1106 76-77 -0.13360261277314933 1106 78-79 -0.3437728670237874 1106 80-81 -0.35449937314099955 1106 82-83 0.03448508750844326 1106 84-85 0.3149816857243479 1106 86-87 0.18738245714524737 1106 88-89 0.37493851665135125 1106 90-91 0.8542402340137549 1106 92-93 1.4533413694637591 1106 94-95 1.6152031559218472 1106 96-97 1.6780744383837725 1106 98-99 2.478757516800103 1106 100-101 1.5524609772181446 1107 1 0.5444703857733657 1107 2 0.4354531734201963 1107 3 0.4283186905200189 1107 4 0.5812576534892884 1107 5 -0.08010918123489574 1107 6 0.4624001547017116 1107 7 0.5447753901139905 1107 8 0.34173984586748674 1107 9 0.7621821190083082 1107 10-11 0.6685096790577632 1107 12-13 0.8013330401547236 1107 14-15 0.9029348712607472 1107 16-17 0.7136421561394926 1107 18-19 0.6798527486289956 1107 20-21 0.7674533113429902 1107 22-23 0.8796493855433738 1107 24-25 0.5995982356400944 1107 26-27 0.7660657340009678 1107 28-29 0.7591844055079875 1107 30-31 1.0392751626146293 1107 32-33 1.2642984882815043 1107 34-35 1.1952131966681492 1107 36-37 0.9593655281249553 1107 38-39 0.21131820502969845 1107 40-41 -0.10213663000882889 1107 42-43 0.47631883242367934 1107 44-45 0.27138600359557685 1107 46-47 -0.464976929081768 1107 48-49 -0.6898704567451901 1107 50-51 -0.10589993559623068 1107 52-53 0.25508718548350373 1107 54-55 0.062334281108508804 1107 56-57 0.22773872019346086 1107 58-59 0.5443902935762388 1107 60-61 0.8044331643128935 1107 62-63 0.4614646636246569 1107 64-65 0.5735963091449285 1107 66-67 0.548215058480487 1107 68-69 0.352039520285949 1107 70-71 0.9222872528871093 1107 72-73 1.3882160049109658 1107 74-75 1.2588079640465644 1107 76-77 1.9443746117008907 1107 78-79 2.0850053611174992 1107 80-81 2.2086737594588826 1107 82-83 2.3407387065009146 1107 84-85 2.265396663527863 1107 86-87 1.894736249849359 1107 88-89 2.187812479206844 1107 90-91 1.7954098923808672 1107 92-93 2.228133880562007 1107 94-95 1.7022712894867968 1107 96-97 1.8066210510068164 1107 98-99 2.9185181807645897 1107 100-101 2.508068002888864 1108 1 -0.4557078673460637 1108 2 -1.0766680387010155 1108 3 -0.8631251597473621 1108 4 -0.8171380684358454 1108 5 -0.9006082899692984 1108 6 -0.7283306830879468 1108 7 -0.7589679253940318 1108 8 -0.4675293163428549 1108 9 -0.8317572749310855 1108 10-11 -1.0275687523147852 1108 12-13 -0.6752266746402853 1108 14-15 -0.804730012874721 1108 16-17 -1.3277125675681773 1108 18-19 -1.1500046488754592 1108 20-21 -1.4298728918655712 1108 22-23 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-1.2720387879710398 2316 92-93 -1.7351114762317383 2316 94-95 -1.976516589301081 2316 96-97 -2.0883936117497814 2316 98-99 -1.5640820147388315 2316 100-101 -1.2647570215490633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3250.0 3 588.0 4 430.0 5 386.0 6 378.0 7 415.0 8 490.0 9 603.0 10 750.0 11 1108.0 12 1696.0 13 2312.0 14 2715.0 15 3508.0 16 4352.0 17 5301.0 18 4352.0 19 2709.0 20 2693.0 21 2763.0 22 2830.0 23 2899.0 24 2915.0 25 3236.0 26 3475.0 27 4106.0 28 4491.0 29 5288.0 30 5899.0 31 6759.0 32 7774.0 33 8875.0 34 10744.0 35 12698.0 36 14489.0 37 11370.0 38 2377.0 39 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09469374734156 24.21775308379413 16.058326244151424 16.62922692471289 2 16.61766069323585 23.662600495357676 40.4954901259586 19.224248685447876 3 18.130881973097736 28.198369439238103 37.47375671397633 16.196991873687836 4 11.116998728274693 23.02087749046206 40.30044510385757 25.56167867740568 5 8.699367969153716 41.680910548422574 36.80619045726173 12.813531025161984 6 21.60719135659351 43.32169661961195 19.395332507502037 15.675779516292504 7 20.40573029466113 43.78323961665574 20.54017895580414 15.27085113287899 8 25.178988919575858 34.528800492757654 20.651446813301806 19.640763774364682 9 26.48903547987575 17.272347950485802 24.496810983727077 31.74180558591137 10-11 22.24223859591026 29.938239920523223 28.49739216822585 19.322129315340675 12-13 24.19048817395854 31.80034635417529 28.316937248967726 15.692228222898446 14-15 21.76438254557977 30.555831495572818 30.967754753342735 16.712031205504672 16-17 15.711977431808698 34.524763424696545 31.193836128972446 18.569423014522314 18-19 15.187316640287152 30.890523903815208 38.75007450281786 15.172084953079782 20-21 16.764575372042042 28.977554953606504 39.37751256697419 14.880357107377263 22-23 17.166405621359576 29.45650698199615 38.332930451569744 15.044156945074521 24-25 15.969319830172807 31.169317180762633 39.03144187525252 13.829921113812038 26-27 15.624472224521899 30.856859011507577 39.608908022187265 13.909760741783261 28-29 15.04690568199504 31.332929345108596 39.66866342813811 13.951501544758251 30-31 16.124695348097912 30.020133517007526 39.70872629013458 14.146444844759987 32-33 14.729616519369399 30.982095848634255 40.20635966582449 14.08192796617185 34-35 14.811834633023727 31.953178032748887 38.8662063941456 14.368780940081791 36-37 16.0133251210321 30.67327624459412 37.49081083229024 15.82258780208354 38-39 14.311113171190378 32.05225832511141 38.25710672167078 15.379521782027428 40-41 14.6162683291011 31.896360500187054 38.694971312411646 14.792399858300199 42-43 15.047145449007429 31.62022751652077 38.12391572088835 15.20871131358345 44-45 15.839256804404611 31.723870788321022 37.17931315325099 15.25755925402338 46-47 16.51712401334958 31.74762939026328 35.60781639031626 16.12743020607088 48-49 15.9647589344246 31.736824331035578 35.98469046531185 16.313726269227967 50-51 15.566503115419506 32.38182263628719 34.37291008654311 17.678764161750195 52-53 16.779725546671965 32.48348810647067 33.819668603400046 16.917117743457315 54-55 16.745183856027175 31.418242252290128 33.89128541963934 17.945288472043355 56-57 17.209071345803675 32.657507035259066 33.290514815427905 16.842906803509354 58-59 17.311995974416355 31.41511182912457 33.09023133863898 18.182660857820093 60-61 16.248696429458214 30.59475923259009 34.50033934217278 18.656204995778914 62-63 16.749489324650472 30.341895904996175 34.14423392076172 18.764380849591625 64-65 16.29643117261471 30.461828775739917 34.13295371780441 19.108786333840957 66-67 16.64194817061059 31.481322086417627 32.905173669504876 18.971556073466903 68-69 16.438347094305144 29.7101257357761 33.62190543663222 20.229621733286542 70-71 16.31180784320217 30.732837822178816 34.13332450462679 18.82202982999222 72-73 17.4911438503559 29.317331567621252 34.368813110412184 18.82271147161066 74-75 16.580587062897703 30.205332962528885 33.74154930043768 19.472530674135726 76-77 16.657066052227343 30.438582552041954 33.80210816250861 19.1022432332221 78-79 16.13856895507169 30.4837796589408 32.96773146967976 20.409919916307743 80-81 16.572864454700152 28.940851635800218 33.66252391956405 20.823759989935574 82-83 16.746840765301226 30.069425361942187 32.70242442501432 20.48130944774227 84-85 16.66815643197897 29.797292599839103 32.645392818006954 20.889158150174964 86-87 16.802727844539344 30.327407554540336 31.975700996457775 20.89416360446254 88-89 17.554256956975184 30.311056665618224 31.46026122800338 20.674425149403213 90-91 17.876974732039088 29.228848720045825 31.846041264490747 21.048135283424337 92-93 18.436621630122065 28.63357512473059 32.23694011938381 20.69286312576353 94-95 18.4239105242595 29.06140530947667 31.421798123340373 21.092886042923457 96-97 19.964771361500794 28.459045052263537 29.888387462048094 21.68779612418757 98-99 19.519543380809544 28.4476536686465 29.088946716725268 22.94385623381869 100-101 20.732471303419725 28.294181795280604 30.099671518209025 20.873675383090646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 745.0 1 465.5 2 191.0 3 188.5 4 144.5 5 137.0 6 203.0 7 334.5 8 461.5 9 511.5 10 485.0 11 374.5 12 334.5 13 386.5 14 440.0 15 498.0 16 570.5 17 654.0 18 745.0 19 858.5 20 1008.5 21 1161.0 22 1294.5 23 1480.5 24 1682.0 25 2044.5 26 3030.5 27 4273.5 28 4620.0 29 4848.5 30 5762.5 31 6365.5 32 6547.5 33 6767.5 34 7396.0 35 8092.0 36 8197.5 37 7921.5 38 6572.0 39 5060.5 40 4470.0 41 4310.5 42 4318.0 43 4468.0 44 4659.5 45 4066.0 46 3054.0 47 2381.5 48 2300.5 49 2272.5 50 2056.5 51 1763.5 52 1390.5 53 1094.0 54 857.0 55 699.0 56 560.0 57 462.5 58 394.5 59 353.0 60 330.5 61 304.0 62 296.5 63 256.0 64 203.5 65 185.5 66 179.0 67 158.0 68 120.5 69 105.0 70 84.0 71 69.0 72 66.0 73 57.5 74 52.0 75 39.5 76 33.5 77 28.5 78 21.0 79 14.5 80 9.0 81 6.0 82 4.5 83 1.5 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3826750175447889 2 0.026482700176109954 3 0.03376544272454019 4 0.04369645529058143 5 0.06620675044027488 6 0.05495160286542816 7 0.03641371274215119 8 0.03641371274215119 9 0.03641371274215119 10-11 0.03608267898994982 12-13 0.026813733928311333 14-15 0.028468902689318204 16-17 0.021848227645290715 18-19 0.027144767680512705 20-21 0.03376544272454019 22-23 0.030786138954727826 24-25 0.04303438778617868 26-27 0.03608267898994982 28-29 0.03144820645913057 30-31 0.03310337522013744 32-33 0.028799936441519576 34-35 0.029462003945922327 36-37 0.032441307715734696 38-39 0.015227552601263225 40-41 0.012248248831450856 42-43 0.013241350088054977 44-45 0.012248248831450856 46-47 0.01721375511447147 48-49 0.01655168761006872 50-51 0.01257928258365223 52-53 0.009599978813839859 54-55 0.008937911309437111 56-57 0.011255147574846731 58-59 0.005296540035221992 60-61 0.009599978813839859 62-63 0.010924113822645357 64-65 0.007944810052832986 66-67 0.005296540035221992 68-69 0.007282742548430239 70-71 0.012910316335853605 72-73 0.011255147574846731 74-75 0.01257928258365223 76-77 0.006620675044027489 78-79 0.00827584380503436 80-81 0.009931012566041232 82-83 0.010924113822645357 84-85 0.0076137763006316125 86-87 0.0046344725308192425 88-89 0.016220653857867348 90-91 0.026813733928311333 92-93 0.010924113822645357 94-95 0.019199957627679717 96-97 0.018868923875478345 98-99 0.01257928258365223 100-101 0.13009626461514015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151042.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.39043444869639 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52733713229632 28.38150977873704 2 7.21161250577545 4.960209742985395 3 2.749114430925612 2.8362971888613764 4 1.465039273063299 2.015333483401968 5 0.9279223779454797 1.5955826856106248 6 0.5871707993223472 1.2115835330570306 7 0.4716617896195903 1.1354457700507143 8 0.398506083474511 1.096383787290952 9 0.3099491760357308 0.9593358138795832 >10 2.595102417988603 18.907323790733706 >50 0.44855998767903893 11.041961838429046 >100 0.28877252425689204 18.34522847949577 >500 0.01155090097027568 2.311939725374399 >1k 0.0077006006468504536 5.201864382092398 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2551 1.6889342037314126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 1.6392791409012064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1539 1.0189218892758307 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1291 0.8547291481839489 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 684 0.45285417301148023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 663 0.4389507554190225 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 567 0.37539227499635863 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 544 0.3601647223950954 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 526 0.3482475073158459 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 508 0.33633029223659644 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 482 0.31911653712212495 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 455 0.30124071450325074 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 451 0.29859244448563976 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 435 0.2879993644151958 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 422 0.27939248685796003 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 400 0.2648270017610995 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 390 0.25820632671707205 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 390 0.25820632671707205 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 379 0.25092358416864186 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 358 0.23702016657618413 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 347 0.22973742402775388 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.22444088399253187 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 329 0.21782020894850437 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 311 0.2059029938692549 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 307 0.20325472385164392 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 306 0.20259265634724113 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 299 0.1979581838164219 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 293 0.19398577879000545 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 292 0.1933237112856027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 290 0.1919995762767972 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 288 0.19067544126799169 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 280 0.1853789012327697 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 279 0.18471683372836695 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 263 0.17412375365792296 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 257 0.17015134863150647 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 256 0.16948928112710374 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 255 0.16882721362270098 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 254 0.16816514611829822 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 249 0.16485480859628449 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.16485480859628449 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 247 0.16353067358747897 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 239 0.158234133552257 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 238 0.15757206604785423 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 237 0.15690999854345147 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 233 0.1542617285258405 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.15359966102143774 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 227 0.150289323499424 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 222 0.14697898597741027 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 217 0.1436686484553965 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 217 0.1436686484553965 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 212 0.14035831093338277 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 211 0.13969624342898002 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 211 0.13969624342898002 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 211 0.13969624342898002 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 203 0.13439970339375804 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.13108936587174427 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 198 0.13108936587174427 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 192 0.12711696084532778 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 191 0.12645489334092505 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 190 0.1257928258365223 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 186 0.1231445558189113 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 186 0.1231445558189113 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 186 0.1231445558189113 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 177 0.11718594827928655 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 177 0.11718594827928655 No Hit GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA 174 0.1151997457660783 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 172 0.11387561075727282 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 170 0.1125514757484673 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 169 0.11188940824406457 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 167 0.11056527323525907 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 164 0.10857907072205082 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 163 0.10791700321764806 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 162 0.10725493571324533 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 162 0.10725493571324533 No Hit GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAG 162 0.10725493571324533 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 159 0.10526873320003709 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 159 0.10526873320003709 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 158 0.10460666569563433 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 156 0.10328253068682884 No Hit GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC 156 0.10328253068682884 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 156 0.10328253068682884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 154 0.10195839567802333 No Hit GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC 153 0.10129632817362057 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 153 0.10129632817362057 No Hit AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA 152 0.10063426066921784 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 152 0.10063426066921784 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.620675044027489E-4 0.0 14-15 0.0 0.0 0.0 6.620675044027489E-4 0.0 16-17 0.0 0.0 0.0 6.620675044027489E-4 0.0 18-19 0.0 0.0 0.0 0.0013241350088054977 0.0 20-21 0.0 0.0 0.0 0.003972405026416493 0.0 22-23 0.0 0.0 0.0 0.015227552601263225 0.0 24-25 0.0 0.0 0.0 0.03442751022894294 0.0 26-27 0.0 0.0 0.0 0.06422054792706663 0.0 28-29 0.0 0.0 0.0 0.13175143337614703 0.0 30-31 0.0 0.0 0.0 0.28998556692840405 0.0 32-33 0.0 0.0 0.0 0.5018471683372836 0.0 34-35 0.0 0.0 0.0 0.8113637266455689 0.0 36-37 0.0 0.0 0.0 1.250976549568994 0.0 38-39 0.0 0.0 0.0 1.7594443929503052 0.0 40-41 0.0 0.0 0.0 2.221567511023424 0.0 42-43 0.0 0.0 0.0 2.6154976761430593 0.0 44-45 0.0 0.0 0.0 3.1070827981621005 0.0 46-47 0.0 0.0 0.0 3.6125713377735993 0.0 48-49 0.0 0.0 0.0 4.1299770924643475 0.0 50-51 0.0 0.0 0.0 4.62189324823559 0.0 52-53 0.0 0.0 0.0 5.1025542564319855 0.0 54-55 0.0 0.0 0.0 5.622608281140345 0.0 56-57 0.0 0.0 0.0 6.246937937792137 0.0 58-59 0.0 0.0 0.0 6.8328676791885705 0.0 60-61 0.0 0.0 0.0 7.454880099574952 0.0 62-63 0.0 0.0 0.0 8.025582288370122 0.0 64-65 0.0 0.0 0.0 8.599925848439508 0.0 66-67 0.0 0.0 0.0 9.261000251585651 0.0 68-69 0.0 0.0 0.0 9.972060751314205 0.0 70-71 0.0 0.0 0.0 10.751976271500642 0.0 72-73 0.0 0.0 0.0 11.51037459779399 0.0 74-75 0.0 0.0 0.0 12.257517776512493 0.0 76-77 0.0 0.0 0.0 13.055640153070007 0.0 78-79 0.0 0.0 0.0 13.844493584565882 0.0 80-81 0.0 0.0 0.0 14.718422690377512 0.0 82-83 0.0 0.0 0.0 15.545345003376545 0.0 84-85 0.0 0.0 0.0 16.338170839898837 0.0 86-87 0.0 0.0 0.0 17.20084479813562 0.0 88-89 0.0 0.0 0.0 18.045311900001323 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTC 20 0.0020658537 71.291016 5 GCATCCG 20 5.357811E-4 47.543102 94-95 TGCTTGG 20 5.379749E-4 47.503727 14-15 GGTATCA 1495 0.0 45.885307 1 GGAGCCT 50 0.0016094391 38.009277 8 GCAGTGC 50 0.0016094391 38.009277 7 GTATCAA 2435 0.0 36.388683 1 GGCAGGC 55 0.0025653061 34.565342 2 GCTGAAT 55 0.0025694813 34.55389 9 AGTGCCT 55 0.0025694813 34.55389 9 GAGCCTG 55 0.0025694813 34.55389 9 GGGCAGG 60 0.0038846347 31.758583 1 GCCGTAT 30 0.00392889 31.695402 94-95 TCACGTG 30 0.00392889 31.695402 94-95 TAGAGGA 75 3.0555463E-4 31.684896 4 ATAGGGT 75 3.0555463E-4 31.684896 3 TAGGGTC 75 3.0555463E-4 31.684896 4 AGGAGCC 60 0.003935473 31.674398 7 CAGTGCC 60 0.003935473 31.674398 8 TGGATAT 60 0.003935473 31.674398 9 >>END_MODULE