##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151042 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.668045973967505 31.0 31.0 34.0 30.0 34.0 2 31.930655049588857 31.0 31.0 34.0 30.0 34.0 3 31.877206339958423 31.0 31.0 34.0 30.0 34.0 4 35.61934428834364 37.0 35.0 37.0 33.0 37.0 5 35.56202910448749 37.0 35.0 37.0 33.0 37.0 6 35.63245322493081 37.0 35.0 37.0 33.0 37.0 7 35.561519312509105 37.0 35.0 37.0 33.0 37.0 8 35.56116841673177 37.0 35.0 37.0 33.0 37.0 9 36.968181035738404 39.0 37.0 39.0 33.0 39.0 10-11 36.96346049443201 39.0 37.0 39.0 33.0 39.0 12-13 36.87502151719389 39.0 37.0 39.0 32.0 39.0 14-15 37.706790164325156 39.5 36.5 41.0 32.0 41.0 16-17 37.766286860608304 39.0 37.0 41.0 32.5 41.0 18-19 38.07302935607315 40.0 37.0 41.0 33.0 41.0 20-21 38.17086638153626 40.0 37.0 41.0 33.0 41.0 22-23 38.01997457660784 40.0 37.0 41.0 33.0 41.0 24-25 37.853921425828574 40.0 37.0 41.0 33.0 41.0 26-27 37.58996835317329 40.0 36.5 41.0 32.0 41.0 28-29 37.345691926748856 39.0 36.0 41.0 32.0 41.0 30-31 36.85819176123198 39.0 35.0 40.5 31.5 41.0 32-33 36.319589915387766 38.0 35.0 40.0 30.5 41.0 34-35 35.95979264045762 38.0 35.0 40.0 30.0 41.0 36-37 35.647237192304125 38.0 35.0 40.0 30.0 41.0 38-39 35.210438156274414 38.0 35.0 40.0 28.0 41.0 40-41 34.896068643158856 38.0 35.0 40.0 26.5 41.0 42-43 34.339044769004644 38.0 34.0 40.0 23.5 41.0 44-45 33.55670277141458 38.0 33.0 40.0 16.0 41.0 46-47 32.52041154116074 38.0 33.0 40.0 2.0 41.0 48-49 31.651123528554972 37.5 31.5 40.0 2.0 41.0 50-51 31.027575111558377 37.0 31.0 40.0 2.0 41.0 52-53 30.432316839024907 37.0 29.5 40.0 2.0 41.0 54-55 29.900766012102594 36.0 28.0 40.0 2.0 41.0 56-57 29.42366030640484 36.0 27.0 39.0 2.0 40.5 58-59 28.80148567947988 35.0 25.5 39.0 2.0 40.0 60-61 28.29559989936574 35.0 23.0 39.0 2.0 40.0 62-63 27.72782073860251 35.0 21.0 38.5 2.0 40.0 64-65 27.016988652162972 34.0 15.5 38.0 2.0 40.0 66-67 26.21939261927146 34.0 7.0 37.5 2.0 39.5 68-69 25.42938056964288 33.0 2.0 37.0 2.0 39.0 70-71 24.832894161888746 32.5 2.0 36.0 2.0 39.0 72-73 24.351971637028115 32.0 2.0 36.0 2.0 38.0 74-75 23.768915268600786 31.0 2.0 35.0 2.0 37.5 76-77 22.699735835065745 29.5 2.0 34.0 2.0 36.0 78-79 23.13552190781372 31.0 2.0 35.0 2.0 36.0 80-81 22.80457422438792 31.0 2.0 34.5 2.0 36.0 82-83 22.28010089908767 30.5 2.0 34.0 2.0 35.5 84-85 21.82444618053257 30.0 2.0 34.0 2.0 35.0 86-87 21.179536155506415 29.0 2.0 34.0 2.0 35.0 88-89 20.456793474662675 29.0 2.0 34.0 2.0 35.0 90-91 19.85107122522212 27.0 2.0 34.0 2.0 35.0 92-93 19.165612875888826 26.0 2.0 33.0 2.0 35.0 94-95 18.27970034824751 25.0 2.0 33.0 2.0 35.0 96-97 17.21520173196859 21.0 2.0 32.5 2.0 34.0 98-99 15.784506958329471 2.0 2.0 32.0 2.0 34.0 100-101 12.101091087247255 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49359275120096413 1101 2 0.663543151636901 1101 3 0.6826811289912094 1101 4 0.35400995708182137 1101 5 0.2953049225983122 1101 6 -0.02053317631968099 1101 7 0.255799108408155 1101 8 0.14708394788031853 1101 9 0.3921160476820944 1101 10-11 0.6745439246382929 1101 12-13 0.7908779710064167 1101 14-15 0.927945848986667 1101 16-17 0.8679365393476033 1101 18-19 0.6889600528481452 1101 20-21 0.8598397007566589 1101 22-23 0.9097090104192986 1101 24-25 0.8595670874253685 1101 26-27 0.7674470623877525 1101 28-29 0.8400219827640782 1101 30-31 1.0925285284480069 1101 32-33 1.0356958916205272 1101 34-35 1.0181482683255823 1101 36-37 1.105421045594177 1101 38-39 1.5429807808404732 1101 40-41 1.7976300163864494 1101 42-43 2.5547937176662785 1101 44-45 2.2947707933968715 1101 46-47 1.8334896343701317 1101 48-49 2.027932902357346 1101 50-51 2.182501115096745 1101 52-53 1.6824707545312947 1101 54-55 1.9925565706095831 1101 56-57 2.1076255097754313 1101 58-59 2.0435558158793974 1101 60-61 1.8795952038032802 1101 62-63 2.3349952704106514 1101 64-65 2.2606932052299484 1101 66-67 1.6740808472552757 1101 68-69 1.539575458030825 1101 70-71 2.0540825445925748 1101 72-73 2.8639547504344343 1101 74-75 2.5740492142619757 1101 76-77 3.007253161344231 1101 78-79 3.437970118629689 1101 80-81 2.7760227557824244 1101 82-83 3.219023079560621 1101 84-85 2.9375217416447548 1101 86-87 3.097006403573552 1101 88-89 3.151092808447011 1101 90-91 3.396570155084099 1101 92-93 4.065907210122372 1101 94-95 4.217353871279457 1101 96-97 3.869887912035498 1101 98-99 4.445463324628058 1101 100-101 4.31388632383422 1102 1 0.003282468451835996 1102 2 0.39159567731729794 1102 3 0.33517503982463737 1102 4 0.2624606613071734 1102 5 0.13296244669023594 1102 6 0.13900298803486777 1102 7 0.5338886985807747 1102 8 0.04595083340399242 1102 9 0.03539678473599395 1102 10-11 0.03658458968117628 1102 12-13 0.18127297259488984 1102 14-15 0.42635737074881064 1102 16-17 0.11706287631678691 1102 18-19 0.016821961139996233 1102 20-21 0.2250308476379459 1102 22-23 0.5010200344582714 1102 24-25 0.5424009326016872 1102 26-27 0.6900352229299571 1102 28-29 0.419366470744599 1102 30-31 0.26082779774802134 1102 32-33 0.7960739494411371 1102 34-35 0.7917901194322283 1102 36-37 0.5459590102240597 1102 38-39 0.5130644406943361 1102 40-41 0.8605072799679405 1102 42-43 0.29142402583106275 1102 44-45 0.3753331146930705 1102 46-47 0.01992403021890965 1102 48-49 0.3905824840580756 1102 50-51 0.32517293549280524 1102 52-53 0.5822907269976767 1102 54-55 0.5871504496734339 1102 56-57 0.553934945336163 1102 58-59 0.7693844720268039 1102 60-61 0.29972122307681204 1102 62-63 0.37761943645957885 1102 64-65 0.550113940165879 1102 66-67 0.4188123944330826 1102 68-69 1.3131378852255722 1102 70-71 1.5195066682820801 1102 72-73 1.3889203334059488 1102 74-75 1.386211662636434 1102 76-77 1.101211649112951 1102 78-79 1.3506567648226344 1102 80-81 1.1550601379702883 1102 82-83 1.5594339659314755 1102 84-85 0.6133662825745425 1102 86-87 0.9850186878052618 1102 88-89 0.8636841459456868 1102 90-91 1.4710168351645798 1102 92-93 0.7662143159150254 1102 94-95 0.833604003652642 1102 96-97 1.2590121310336997 1102 98-99 1.9374944588015204 1102 100-101 2.265887806413909 1103 1 0.367563392088897 1103 2 0.19594558629497527 1103 3 0.11590705093894016 1103 4 0.10230927680971291 1103 5 0.3461463117855672 1103 6 0.6099716571926734 1103 7 0.38039631571213306 1103 8 0.14780002378437018 1103 9 0.14256359512211958 1103 10-11 0.4502331476959327 1103 12-13 0.269037655933019 1103 14-15 0.5457403352022041 1103 16-17 0.5548323503035633 1103 18-19 0.9707359210901814 1103 20-21 0.4618805170831948 1103 22-23 0.8753373670250966 1103 24-25 0.7544829485066415 1103 26-27 0.9054706928925853 1103 28-29 0.7347588248693384 1103 30-31 0.31594421051030963 1103 32-33 0.2515927766903445 1103 34-35 0.712025819345989 1103 36-37 0.8735914716593385 1103 38-39 0.6752758041129425 1103 40-41 0.8486504245497102 1103 42-43 0.6681127294815354 1103 44-45 0.44908515071874433 1103 46-47 0.06299196161682374 1103 48-49 -0.09380000133044675 1103 50-51 -0.32609179575180747 1103 52-53 0.0825781659169067 1103 54-55 -0.15101664815161087 1103 56-57 0.20268458603751682 1103 58-59 -0.2244355912097582 1103 60-61 -0.46340515423467465 1103 62-63 -0.5423767310214984 1103 64-65 0.03119087798325637 1103 66-67 -0.24124317709130594 1103 68-69 -0.2337865183386718 1103 70-71 0.230774273915884 1103 72-73 -0.022189180822277166 1103 74-75 0.004947889521552895 1103 76-77 -0.07491654864502806 1103 78-79 0.43564287194153906 1103 80-81 0.46954226885080885 1103 82-83 0.3212429148631628 1103 84-85 0.7595768794893658 1103 86-87 0.32328638925203634 1103 88-89 0.1527039792311129 1103 90-91 0.7809238965806955 1103 92-93 0.6208660357578886 1103 94-95 0.6580985902196623 1103 96-97 0.8620110770909939 1103 98-99 1.5147436683444937 1103 100-101 2.5168938426312124 1104 1 -0.17030208522785983 1104 2 0.17193333238679287 1104 3 0.14829881559181857 1104 4 0.11559936105533097 1104 5 -0.029895495665890337 1104 6 -0.0979618473349646 1104 7 -0.30064166239301926 1104 8 0.029721004097019943 1104 9 -0.5238327647463308 1104 10-11 0.06738861915604133 1104 12-13 -0.024538052496048124 1104 14-15 0.16336884355919068 1104 16-17 0.3729158751042121 1104 18-19 -0.0023460269208186446 1104 20-21 0.21225218893074072 1104 22-23 0.3695257708634543 1104 24-25 0.22330756444572586 1104 26-27 0.25761287426845314 1104 28-29 0.42633876810491245 1104 30-31 0.5746278164029945 1104 32-33 0.4326983314950468 1104 34-35 0.23148790881754167 1104 36-37 1.1889494029113123 1104 38-39 0.6820785252013764 1104 40-41 -0.21656109051988892 1104 42-43 -1.020020802076779 1104 44-45 -1.116621926814254 1104 46-47 -1.2959979919805136 1104 48-49 -0.7668188777551883 1104 50-51 -0.800818110118616 1104 52-53 -0.5390598932802817 1104 54-55 -0.8692668621444923 1104 56-57 -0.8112362140706075 1104 58-59 -0.7436273738830899 1104 60-61 -0.7342075149140435 1104 62-63 -0.3994069603317669 1104 64-65 -0.15854196879784155 1104 66-67 -0.08417240176130747 1104 68-69 0.23620942705935732 1104 70-71 -0.5076363221106064 1104 72-73 0.13276419125503125 1104 74-75 0.32341434895300836 1104 76-77 -0.15708981530268318 1104 78-79 -0.29247354018622573 1104 80-81 0.0187155805595971 1104 82-83 -0.16391273680147833 1104 84-85 -0.26486336018266243 1104 86-87 -0.44805658502376744 1104 88-89 -0.0914506362574663 1104 90-91 -0.40711953271154755 1104 92-93 -0.19642909421462562 1104 94-95 0.32010246461710423 1104 96-97 -0.5340109078619228 1104 98-99 0.420541749166226 1104 100-101 0.16400831756025802 1105 1 0.14974293057095522 1105 2 0.26170522348318315 1105 3 0.10830400972245613 1105 4 0.11217989172234155 1105 5 0.041090956310043225 1105 6 0.2874426455880297 1105 7 -0.24545402442392117 1105 8 0.06123206326682862 1105 9 0.09721212119922029 1105 10-11 -0.03155956939090032 1105 12-13 0.1530579307095934 1105 14-15 -0.028489356674548105 1105 16-17 0.138465803684916 1105 18-19 0.5154871549170394 1105 20-21 -0.04492220400523905 1105 22-23 0.11509993872044078 1105 24-25 0.058004357070068124 1105 26-27 -0.22191359415556633 1105 28-29 -0.2675754421708021 1105 30-31 -0.49391306573352267 1105 32-33 -0.4149405032883138 1105 34-35 0.2931249256551851 1105 36-37 -0.3327577346898707 1105 38-39 -0.24602604996201904 1105 40-41 0.08054318165255125 1105 42-43 -0.8032091325966277 1105 44-45 -0.4668145424918677 1105 46-47 -0.36224479975439294 1105 48-49 -0.40058938375008424 1105 50-51 -0.252729117347112 1105 52-53 -0.4406311177965776 1105 54-55 -0.864635428997282 1105 56-57 -0.7591001278480398 1105 58-59 -0.44238943941570597 1105 60-61 -0.18456028295282678 1105 62-63 -0.4532744234317967 1105 64-65 0.1441027093824836 1105 66-67 0.47004133671421755 1105 68-69 -0.03637422008603508 1105 70-71 0.19809453535379262 1105 72-73 0.2545881967954351 1105 74-75 0.05156653698053759 1105 76-77 0.153107994505568 1105 78-79 0.3838888369942275 1105 80-81 0.8576975309556509 1105 82-83 0.8154405939347917 1105 84-85 1.1760101194520196 1105 86-87 1.307213293347008 1105 88-89 0.9334162641450803 1105 90-91 1.139800780667663 1105 92-93 1.2589879511907291 1105 94-95 1.0776664173505175 1105 96-97 0.921168073222784 1105 98-99 0.9471833108681693 1105 100-101 1.3506940293725744 1106 1 0.38957474440051953 1106 2 0.13502440253484593 1106 3 0.26704011562978636 1106 4 0.4177042245977418 1106 5 0.2922878257159809 1106 6 0.5765646638220758 1106 7 0.22376041294337767 1106 8 0.4785335910966424 1106 9 0.4638080164575342 1106 10-11 0.2749222590092515 1106 12-13 0.09021708311789922 1106 14-15 0.2871719396911274 1106 16-17 0.40723063445798857 1106 18-19 0.5020197722102466 1106 20-21 0.48904807200324996 1106 22-23 0.7180515227510753 1106 24-25 0.6253829008442651 1106 26-27 0.6136469570288128 1106 28-29 0.7065513635408607 1106 30-31 0.8774286769083304 1106 32-33 1.2061618654723603 1106 34-35 1.1505413529479682 1106 36-37 0.8070157968034053 1106 38-39 0.6820785252013764 1106 40-41 0.27624694086325263 1106 42-43 0.527855352645453 1106 44-45 0.4614773687524547 1106 46-47 0.2364396846550818 1106 48-49 -0.22160016598229504 1106 50-51 -0.07742417583759931 1106 52-53 0.5002955713504207 1106 54-55 -0.05317878689245603 1106 56-57 -0.13333517218424262 1106 58-59 -0.420262966745959 1106 60-61 -0.4553025501357091 1106 62-63 -0.5250449575477809 1106 64-65 0.19441285467202363 1106 66-67 -0.04987873322458469 1106 68-69 -0.05433766873513335 1106 70-71 -0.19916203181659853 1106 72-73 -0.058845881719197024 1106 74-75 -0.37262479560344985 1106 76-77 -0.20561978535372205 1106 78-79 0.16899353704834752 1106 80-81 0.235866632991371 1106 82-83 0.7566170645230272 1106 84-85 1.3552699337811482 1106 86-87 1.5052483460092603 1106 88-89 1.350676161734686 1106 90-91 1.4147685172315043 1106 92-93 0.8006181913972163 1106 94-95 0.7960378423536376 1106 96-97 1.236225731105467 1106 98-99 0.7118059327406954 1106 100-101 0.5824079463618776 1107 1 0.21011132035111046 1107 2 -0.0999109381329788 1107 3 0.03415071203974307 1107 4 0.16007056314242618 1107 5 -0.11458343465846355 1107 6 -0.03269995690751415 1107 7 -0.02727220624210247 1107 8 0.05243824270829833 1107 9 0.26488888887598705 1107 10-11 0.328155225618012 1107 12-13 -0.011172609991540128 1107 14-15 0.28167107113294065 1107 16-17 0.1117278357704734 1107 18-19 0.08310450488733068 1107 20-21 0.09447173538869436 1107 22-23 0.06352536949881227 1107 24-25 -0.1648120422169228 1107 26-27 -0.006464990471663157 1107 28-29 -0.09597710586658792 1107 30-31 0.41458366629262144 1107 32-33 0.009539591780018952 1107 34-35 0.03156818174549869 1107 36-37 0.09047458854245605 1107 38-39 -0.9361032929804409 1107 40-41 -0.4638418450854118 1107 42-43 -0.3399886929055995 1107 44-45 -0.16241763221260186 1107 46-47 0.052787879984173 1107 48-49 -0.29571713181900705 1107 50-51 -0.4917071327957139 1107 52-53 -0.35774341726182257 1107 54-55 -0.13011374153441935 1107 56-57 -0.8576741028926023 1107 58-59 -0.6610465992493353 1107 60-61 -0.5816488153354804 1107 62-63 -0.20663152384772232 1107 64-65 -0.3395574213364725 1107 66-67 -0.19591231747472904 1107 68-69 -0.1361365492601294 1107 70-71 -0.10255905941745169 1107 72-73 0.20230061509608888 1107 74-75 -0.0380947820925428 1107 76-77 -0.2345331225473153 1107 78-79 -0.43560017073007273 1107 80-81 -0.09007430505147695 1107 82-83 0.3354049433109054 1107 84-85 -0.2698603499478658 1107 86-87 0.30887698912834693 1107 88-89 0.8184430021129927 1107 90-91 1.0762238347377782 1107 92-93 0.6202476065680322 1107 94-95 0.4828357578139766 1107 96-97 0.49627205420911835 1107 98-99 1.3843787951224762 1107 100-101 1.3515258772632475 1108 1 0.07784750573435417 1108 2 -0.1899287634449216 1108 3 0.03575499011460792 1108 4 -0.08128416056523946 1108 5 -0.14060839009518133 1108 6 -0.302753432843339 1108 7 -0.20819912246314232 1108 8 -0.030271204707027266 1108 9 -0.7962519310883636 1108 10-11 -0.15214780468502198 1108 12-13 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2.0 4 8.0 5 18.0 6 42.0 7 114.0 8 205.0 9 389.0 10 628.0 11 958.0 12 1350.0 13 1879.0 14 2407.0 15 3277.0 16 4463.0 17 5366.0 18 4764.0 19 3102.0 20 2714.0 21 2734.0 22 2739.0 23 2882.0 24 3070.0 25 2988.0 26 2822.0 27 3092.0 28 3531.0 29 4144.0 30 4906.0 31 5744.0 32 6663.0 33 8356.0 34 10576.0 35 13860.0 36 17603.0 37 18457.0 38 5132.0 39 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.556523121138476 25.429610155686085 16.55662907508824 17.4572376480872 2 15.912251195190105 23.96935545814517 40.743070546013165 19.37532280065156 3 17.334913467777174 28.767495133803845 38.35555673256445 15.542034665854532 4 10.6453834032918 24.19062247586764 39.13481018524649 26.029183935594073 5 7.928920432727321 42.87284331510441 36.877822062737515 12.320414189430755 6 20.6406165172601 44.11885435839038 18.83251016273619 16.408018961613326 7 19.585280915242116 44.04999933793249 19.67002555580567 16.694694191019714 8 24.736166099495506 35.180943048953274 20.69424398511672 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14.618450497212695 30.64809788005985 40.365262642179 14.368188980548457 34-35 14.032555475735675 32.15027030248056 39.62008680135995 14.19708742042381 36-37 15.78374155447344 30.88654442653178 37.49267585398715 15.83703816500763 38-39 13.649500918706858 31.914717518332754 38.66017778219199 15.775603780768401 40-41 14.126570041911926 31.80406671478041 39.05986188265985 15.009501360647814 42-43 15.005726997662855 31.222068472381302 38.54832195658075 15.22388257337509 44-45 15.273285818798144 31.67639357395153 37.63137945531526 15.418941151935064 46-47 16.52752214615802 31.38001350617709 35.39975635915838 16.69270798850651 48-49 15.655910276611804 31.12445544947763 36.27434753247441 16.945286741436156 50-51 14.689953787688193 31.85537797433826 34.974377987579615 18.48029025039393 52-53 16.193509090186836 32.32743210497742 34.45432396287125 17.024734841964488 54-55 16.113729955906305 31.4064962063532 33.65520848505714 18.824565352683358 56-57 16.98964526423114 32.872644694853086 32.61510043564042 17.522609605275356 58-59 16.529508348671232 32.538631638881895 32.845499927172575 18.086360085274293 60-61 15.79659962129739 32.833582712093325 32.81736205823545 18.55245560837383 62-63 15.447690046477138 32.69554163742535 32.78260351425432 19.074164801843196 64-65 15.20669747487454 32.67799684855868 32.64820381086055 19.467101865706226 66-67 15.74070702422844 33.5735542880599 31.39700016220708 19.28873852550458 68-69 15.465719459350307 31.311800477355412 32.25459396651891 20.967886096775366 70-71 15.50826922312999 31.93151573734458 32.731293282663096 19.828921756862332 72-73 16.65927357953417 30.118443876537654 33.045113279750005 20.177169264178175 74-75 15.458614160299783 30.991048847340473 32.42210775810702 21.128229234252725 76-77 15.07924948027701 30.947683425802097 33.599925848439504 20.37314124548139 78-79 14.50887832523404 30.85962844771653 32.83887925212854 21.792613974920883 80-81 14.868049946372533 29.331907681307186 33.440036546126244 22.360005826194037 82-83 14.734225153022884 29.99414060560317 33.00527345495715 22.266360786416797 84-85 14.48808053789035 29.813387007604224 32.82263323942543 22.875899215079997 86-87 14.54142183232641 30.407349168252917 32.0 23.051228999420672 88-89 14.952794586936083 30.34487096304339 31.260841355384596 23.44149309463593 90-91 14.96116948378255 28.87129984573725 32.671923517455525 23.495607153024675 92-93 14.93662337834304 28.44714863133642 32.968422585845616 23.647805404474926 94-95 14.866725811363727 28.545702519828918 32.72202433760146 23.86554733120589 96-97 15.942585506018196 28.519219819652815 31.118827875690208 24.41936679863879 98-99 15.353676460851947 28.262006594192346 30.47993273394155 25.904384211014154 100-101 15.48873823175011 28.61521960779121 31.789171223897988 24.106870936560693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 468.0 2 177.0 3 195.5 4 142.5 5 110.0 6 185.0 7 358.0 8 495.5 9 534.0 10 449.5 11 348.5 12 364.0 13 387.5 14 420.0 15 499.0 16 571.5 17 617.0 18 694.0 19 803.5 20 935.0 21 1085.5 22 1235.5 23 1352.5 24 1479.0 25 1939.5 26 2997.0 27 4154.5 28 4406.0 29 4662.0 30 5649.5 31 6280.5 32 6453.5 33 6672.0 34 7388.0 35 8227.5 36 8439.0 37 8054.5 38 6657.0 39 5190.5 40 4723.0 41 4663.5 42 4627.5 43 4790.0 44 5066.0 45 4628.0 46 3585.5 47 2807.0 48 2823.5 49 2760.5 50 2445.0 51 2117.5 52 1366.5 53 728.5 54 451.0 55 350.5 56 295.0 57 233.5 58 204.0 59 185.0 60 147.5 61 115.0 62 86.5 63 59.5 64 47.5 65 39.5 66 32.0 67 22.0 68 19.5 69 23.0 70 18.5 71 16.5 72 16.5 73 12.0 74 10.0 75 11.0 76 9.5 77 6.5 78 5.0 79 7.0 80 9.0 81 4.5 82 1.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021848227645290715 2 0.013241350088054977 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.62067504402749E-4 26-27 0.005296540035221992 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004965506283020616 36-37 3.310337522013745E-4 38-39 0.009599978813839859 40-41 0.007282742548430239 42-43 0.0019862025132082466 44-45 3.310337522013745E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.310337522013745E-4 68-69 9.931012566041233E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016551687610068721 84-85 0.004965506283020616 86-87 0.00297930376981237 88-89 0.0 90-91 6.62067504402749E-4 92-93 3.310337522013745E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151042.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.595079514307276 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36735196080625 23.488830921200726 2 8.467372094584015 5.011851008328809 3 3.1833739737366056 2.8263661762953354 4 1.6599181226370774 1.9650163530673588 5 1.060378962439319 1.5690999854345147 6 0.7382385181539562 1.310893658717443 7 0.628621283640187 1.302286781160207 8 0.44294311089237376 1.0487149269739542 9 0.36464508623968145 0.9712530289588326 >10 3.1386322453636386 19.433005389229486 >50 0.5503232589874947 11.414705843407793 >100 0.3668821726583298 19.495239734643345 >500 0.02013377776783517 3.724129712265463 >1k 0.011185432093241762 6.438606480316733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2638 1.7465340766144515 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 1.7074720938546895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 1.1553077951827968 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1482 0.9811840415248738 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1281 0.8481084731399213 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 934 0.6183710491121674 TruSeq Adapter, Index 1 (95% over 21bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 688 0.45550244302909126 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.4323300803749951 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 612 0.40518531269448227 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 571 0.3780405450139696 RNA PCR Primer, Index 1 (95% over 23bp) ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 563 0.37274400497874766 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 560 0.3707578024655394 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 539 0.35685438487308163 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 505 0.3343440897233882 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 489 0.32375100965294423 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 473 0.3131579295825002 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 459 0.3038889845208617 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 430 0.28468902689318204 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 384 0.25423392169065556 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 380 0.2515856516730446 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 380 0.2515856516730446 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 373 0.24695117914222534 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 362 0.2396684365937951 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 343 0.22708915401014285 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 331 0.2191443439573099 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 329 0.21782020894850437 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 324 0.21450987142649067 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 323 0.21384780392208788 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 315 0.20855126388686593 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 310 0.20524092636485217 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.20457885886044938 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 306 0.20259265634724113 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 302 0.19994438632963016 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 299 0.1979581838164219 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 0.19663404880761642 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 296 0.1959719813032137 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 287 0.19001337376358893 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 278 0.1840547662239642 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 275 0.18206856371075594 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 265 0.17544788866672845 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 259 0.17147548364031198 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 259 0.17147548364031198 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 250 0.16551687610068724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 245 0.16220653857867348 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 242 0.16022033606546524 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 242 0.16022033606546524 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 239 0.158234133552257 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 230 0.15227552601263225 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 229 0.1516134585082295 No Hit GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT 228 0.15095139100382676 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 226 0.14962725599502125 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 223 0.14764105348181303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 219 0.14499278346420202 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 216 0.14300658095099378 No Hit GGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTT 215 0.14234451344659102 No Hit ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT 211 0.13969624342898002 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 210 0.13903417592457726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 208 0.13771004091577177 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 208 0.13771004091577177 No Hit ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC 199 0.13175143337614703 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 198 0.13108936587174427 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 197 0.13042729836734152 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 197 0.13042729836734152 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 195 0.12910316335853603 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 195 0.12910316335853603 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 187 0.12380662332331403 No Hit AAGAGGGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATT 182 0.12049628580130031 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 181 0.11983421829689755 TruSeq Adapter, Index 1 (96% over 26bp) GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 177 0.11718594827928655 No Hit TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA 177 0.11718594827928655 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.11387561075727282 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 171 0.11321354325287006 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 170 0.1125514757484673 TruSeq Adapter, Index 1 (95% over 24bp) CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA 168 0.11122734073966181 No Hit GCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTA 166 0.10990320573085632 No Hit AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA 166 0.10990320573085632 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 166 0.10990320573085632 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 165 0.10924113822645357 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 164 0.10857907072205082 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 163 0.10791700321764806 No Hit AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT 161 0.10659286820884258 No Hit GGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGG 160 0.10593080070443982 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 160 0.10593080070443982 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 160 0.10593080070443982 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 160 0.10593080070443982 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 160 0.10593080070443982 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 159 0.10526873320003709 No Hit ATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTG 156 0.10328253068682884 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 154 0.10195839567802333 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 154 0.10195839567802333 No Hit CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC 152 0.10063426066921784 No Hit CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT 152 0.10063426066921784 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.620675044027489E-4 0.0 14-15 0.0 0.0 0.0 6.620675044027489E-4 0.0 16-17 0.0 0.0 0.0 6.620675044027489E-4 0.0 18-19 0.0 0.0 0.0 0.0013241350088054977 0.0 20-21 0.0 0.0 0.0 0.003972405026416493 0.0 22-23 0.0 0.0 0.0 0.014565485096860476 0.0 24-25 0.0 0.0 0.0 0.0337654427245402 0.0 26-27 0.0 0.0 0.0 0.06355848042266389 0.0 28-29 0.0 0.0 0.0 0.13142039962394564 0.0 30-31 0.0 0.0 0.0 0.29230280319381363 0.0 32-33 0.0 0.0 0.0 0.5051575058592974 0.0 34-35 0.0 0.0 0.0 0.8169913004329921 0.0 36-37 0.0 0.0 0.0 1.2612385958872365 0.0 38-39 0.0 0.0 0.0 1.7723547092861587 0.0 40-41 0.0 0.0 0.0 2.2364640298724856 0.0 42-43 0.0 0.0 0.0 2.632711431257531 0.0 44-45 0.0 0.0 0.0 3.126613789541982 0.0 46-47 0.0 0.0 0.0 3.635412666675494 0.0 48-49 0.0 0.0 0.0 4.1501701513486315 0.0 50-51 0.0 0.0 0.0 4.640100104606665 0.0 52-53 0.0 0.0 0.0 5.123740416572874 0.0 54-55 0.0 0.0 0.0 5.6394910025026155 0.0 56-57 0.0 0.0 0.0 6.258193085366984 0.0 58-59 0.0 0.0 0.0 6.852067636816249 0.0 60-61 0.0 0.0 0.0 7.47838349598125 0.0 62-63 0.0 0.0 0.0 8.05140292104183 0.0 64-65 0.0 0.0 0.0 8.623098211093604 0.0 66-67 0.0 0.0 0.0 9.288145019266164 0.0 68-69 0.0 0.0 0.0 9.999205518994717 0.0 70-71 0.0 0.0 0.0 10.784417579216377 0.0 72-73 0.0 0.0 0.0 11.549767614305953 0.0 74-75 0.0 0.0 0.0 12.31677281815654 0.0 76-77 0.0 0.0 0.0 13.134757219846136 0.0 78-79 0.0 0.0 0.0 13.948769216509316 0.0 80-81 0.0 0.0 0.0 14.85315342752347 0.0 82-83 0.0 0.0 0.0 15.724103229565287 0.0 84-85 0.0 0.0 0.0 16.547384171290105 0.0 86-87 0.0 0.0 0.0 17.440182200977212 0.0 88-89 0.0 0.0 0.0 18.327683690629094 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATATGG 25 0.0050064246 57.01848 2 GTATATG 25 0.0050064246 57.01848 1 GACCGCG 25 0.0050129946 56.9996 7 TGACCGC 25 0.0050129946 56.9996 6 CCATAGG 95 0.0 55.017834 1 TAGGGTC 90 3.6379788E-12 52.777412 4 CATAGGG 100 0.0 52.26694 2 AGGGTCT 95 7.2759576E-12 49.99965 5 GGTATCA 1485 0.0 47.675385 1 GTATAGT 40 5.364646E-4 47.5154 1 ATAGGGT 105 2.0008883E-11 45.23778 3 GGTCTTC 105 1.0149961E-9 40.714005 7 TCAGGAA 25 0.0016139855 37.999733 72-73 TCGTATC 25 0.0016139855 37.999733 94-95 GTATCAA 2310 0.0 35.585125 1 GTTATAT 55 0.0025684757 34.556656 1 TGGGCAA 55 0.0025726531 34.54521 8 GGGTAAT 70 2.0450105E-4 33.928333 7 TCAACGT 30 0.0039465157 31.666447 94-95 GTAATCC 65 0.0058284174 29.230566 9 >>END_MODULE