FastQCFastQC Report
Thu 26 May 2016
SRR938359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379884
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110970.32832487742182864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87130.2577899123165174No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA69210.20477034123064577No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA66520.19681148820492061No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65980.19521380023693122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63430.18766916261031444No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58560.1732603840841875No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53780.1591178868860588No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49990.14790448429591074No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49850.1474902688968024No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47950.14186877419461733No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA46830.13855505100175036No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41680.12331784167740668No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39240.11609865900723221No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA36270.10731137518329031No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA36170.10701550704107005No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG35080.10379054429086915No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33830.10009219251311584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.059.3219221
GTATCAA112850.043.326211
ATCAACG144300.033.554213
TATCAAC145750.033.350932
TCAACGC146900.032.8955764
CAACGCA150050.032.14165
AACGCAG156800.030.88496
ACGCAGA179300.026.7212187
CGCAGAG179650.026.5360818
TACATGG132150.025.7698212
GTACATG136700.025.7119521
AGGTAAG22100.025.3954142
GTGTAAG14800.025.3877681
TAAGGTA15500.025.162154
TAAGGTG26050.024.6485635
TGGTATC26450.024.6354482
ACATGGG136200.024.1654323
GCAGAGT196800.023.5221869
GTGGTAT30000.022.829711
GAGTACT119950.022.18589412-13
Produced by FastQC (version 0.11.5)