##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938359_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3379884 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15236706348502 31.0 31.0 34.0 28.0 34.0 2 31.19184770838289 31.0 31.0 34.0 28.0 34.0 3 31.233776366289494 31.0 31.0 34.0 27.0 34.0 4 34.8988651681537 37.0 35.0 37.0 32.0 37.0 5 34.87901803730542 37.0 35.0 37.0 32.0 37.0 6 34.902191909544825 37.0 35.0 37.0 32.0 37.0 7 34.81246072350412 37.0 35.0 37.0 32.0 37.0 8 34.77230046948357 37.0 35.0 37.0 32.0 37.0 9 36.27233183150664 39.0 35.0 39.0 32.0 39.0 10-11 36.118612206809466 39.0 35.0 39.0 31.0 39.0 12-13 35.98928439555914 38.0 35.0 39.0 30.0 39.0 14-15 36.95968130858929 39.0 36.0 41.0 31.0 41.0 16-17 36.792410775044345 39.0 36.0 41.0 30.0 41.0 18-19 36.949986745107225 39.0 36.0 41.0 31.0 41.0 20-21 36.929454531575644 39.0 36.0 41.0 30.0 41.0 22-23 36.8495346289991 39.0 36.0 41.0 30.0 41.0 24-25 36.71346087617208 39.0 36.0 41.0 30.0 41.0 26-27 36.50512754283875 39.0 36.0 41.0 30.0 41.0 28-29 36.30223892299262 39.0 35.0 40.5 29.0 41.0 30-31 36.03041450534989 38.5 35.0 40.0 28.5 41.0 32-33 35.74065115844212 38.0 35.0 40.0 27.0 41.0 34-35 35.49147455948192 38.0 34.5 40.0 27.0 41.0 36-37 35.20398910140111 38.0 34.0 40.0 25.5 41.0 38-39 35.046865069925474 38.0 34.0 40.0 25.0 41.0 40-41 34.934801756509984 38.0 34.0 40.0 25.0 41.0 42-43 34.62395943174381 38.0 33.5 40.0 24.0 41.0 44-45 34.28209977028797 38.0 33.0 40.0 23.5 41.0 46-47 33.87708468693008 38.0 33.0 40.0 22.5 41.0 48-49 33.497115581481495 38.0 32.5 40.0 19.5 41.0 50-51 32.77300715054126 36.5 31.5 39.5 18.0 40.5 52-53 32.77247281267641 37.0 31.5 39.0 18.0 40.0 54-55 32.87217593858251 37.0 32.0 40.0 16.0 41.0 56-57 32.55232516855608 37.0 31.0 40.0 13.5 41.0 58-59 32.17345565705806 36.0 31.0 39.0 8.5 41.0 60-61 31.706973819219833 36.0 30.5 39.0 7.5 40.5 62-63 31.152146641719064 35.0 29.5 39.0 4.5 40.0 64-65 30.644965478105163 35.0 29.0 38.0 2.0 40.0 66-67 30.123701582657866 34.5 28.5 38.0 2.0 40.0 68-69 29.554779394795798 34.0 28.0 37.5 2.0 39.5 70-71 28.920208504197184 34.0 26.5 37.0 2.0 39.0 72-73 28.30781426226462 34.0 26.0 36.0 2.0 39.0 74-75 27.661826263859943 33.0 25.5 35.5 2.0 38.5 76-77 26.93316575361758 32.5 24.5 35.0 2.0 37.5 78-79 26.175191071646246 32.0 21.5 35.0 2.0 37.0 80-81 25.512055443322907 31.5 20.0 35.0 2.0 36.0 82-83 24.79096235255411 31.0 17.5 34.5 2.0 36.0 84-85 24.10891557225041 31.0 10.5 34.0 2.0 35.0 86-87 23.37442927035366 30.0 4.5 34.0 2.0 35.0 88-89 22.550581321725836 29.5 2.0 34.0 2.0 35.0 90-91 21.645337532294008 29.0 2.0 34.0 2.0 35.0 92-93 20.72187359092797 28.0 2.0 34.0 2.0 35.0 94-95 19.728977828824895 26.5 2.0 33.0 2.0 35.0 96-97 18.499951181756536 25.0 2.0 33.0 2.0 34.5 98-99 16.978825160863508 19.0 2.0 32.0 2.0 34.0 100-101 13.418673540275346 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4700621758829513 1101 2 0.4736796338711713 1101 3 0.43118724173276846 1101 4 0.3362103734463773 1101 5 0.3801028654913168 1101 6 0.5555447846659263 1101 7 0.4740238807164161 1101 8 0.6552346867924186 1101 9 0.7641014902535801 1101 10-11 0.6521311800012342 1101 12-13 0.7779342200498505 1101 14-15 0.8807277069043025 1101 16-17 0.7164140641000003 1101 18-19 0.7253062924070548 1101 20-21 0.8338923154462563 1101 22-23 0.7911480661653201 1101 24-25 0.7865487282688761 1101 26-27 0.6851964910438753 1101 28-29 0.8023624400477942 1101 30-31 0.7836818196047872 1101 32-33 0.80397855109603 1101 34-35 0.6775397926239606 1101 36-37 0.7433486535392433 1101 38-39 0.7378384881626161 1101 40-41 0.7376855004732903 1101 42-43 1.076533820167299 1101 44-45 1.1603638139199433 1101 46-47 1.0194330587671772 1101 48-49 0.9172439360113955 1101 50-51 1.0970002919660828 1101 52-53 0.9243444410909021 1101 54-55 0.8792578570913676 1101 56-57 0.805872523961014 1101 58-59 0.8416085302151473 1101 60-61 1.0413557886434113 1101 62-63 0.9427901016480824 1101 64-65 0.8318651182806107 1101 66-67 0.844032913572196 1101 68-69 0.9851052245660448 1101 70-71 1.081910950305602 1101 72-73 1.1827077420843395 1101 74-75 1.036100826910964 1101 76-77 1.0745406717390509 1101 78-79 1.169813917640564 1101 80-81 1.2254841047126774 1101 82-83 1.288419653309802 1101 84-85 1.3822040267249776 1101 86-87 1.3821774058700456 1101 88-89 1.4147965274711716 1101 90-91 1.2865722942397824 1101 92-93 1.1253963066485895 1101 94-95 1.1225372141513077 1101 96-97 1.1040392056731534 1101 98-99 1.1705348853554831 1101 100-101 0.9396570612099335 1102 1 0.39376774845858975 1102 2 0.33733855139384517 1102 3 0.25685432889562065 1102 4 0.18370922208777074 1102 5 0.3270488617669187 1102 6 0.3335346126629659 1102 7 0.3702486259157496 1102 8 0.4538941049057925 1102 9 0.5669390385605766 1102 10-11 0.669550034249248 1102 12-13 0.6312853744119948 1102 14-15 0.6718049780842961 1102 16-17 0.737388513950826 1102 18-19 0.699131906631834 1102 20-21 0.624853749938957 1102 22-23 0.6421305254672944 1102 24-25 0.6018394713457056 1102 26-27 0.6442666938832247 1102 28-29 0.5494132600153492 1102 30-31 0.7153230562640402 1102 32-33 0.6939765487332394 1102 34-35 0.6638569468659625 1102 36-37 0.8970368055586277 1102 38-39 0.814431267642405 1102 40-41 0.8078492937493564 1102 42-43 1.063859564545666 1102 44-45 1.1087738377480676 1102 46-47 1.1223926510861162 1102 48-49 1.035933389566587 1102 50-51 0.9858654528559327 1102 52-53 0.8691547172167233 1102 54-55 0.8113950790132307 1102 56-57 1.0286120566095462 1102 58-59 1.0611221562939122 1102 60-61 0.9794391269826512 1102 62-63 1.0063383886267196 1102 64-65 1.0358278943563803 1102 66-67 0.7217041656496477 1102 68-69 0.9483996119431488 1102 70-71 1.1295805003746828 1102 72-73 1.1685566437883494 1102 74-75 1.1948270238233185 1102 76-77 1.2063330867888098 1102 78-79 1.24398323743792 1102 80-81 1.4168859589006182 1102 82-83 1.5270328168427731 1102 84-85 1.4814004785381165 1102 86-87 1.6939344792165976 1102 88-89 1.5539419190332175 1102 90-91 1.44774838202337 1102 92-93 1.499409442148476 1102 94-95 1.6037793799069036 1102 96-97 1.5252449284260052 1102 98-99 1.527377960183781 1102 100-101 1.3678173903983772 1103 1 0.39387454622637463 1103 2 0.3852653030711579 1103 3 0.3369441411831744 1103 4 0.29367554437184396 1103 5 0.3280242309286834 1103 6 0.4538831158198846 1103 7 0.3411529031427065 1103 8 0.31091942099029524 1103 9 0.4552677357924111 1103 10-11 0.6565502202230391 1103 12-13 0.5650581299514315 1103 14-15 0.7021136926875542 1103 16-17 0.7389404870041147 1103 18-19 0.7816504931326307 1103 20-21 0.5438966905875873 1103 22-23 0.6309388950825365 1103 24-25 0.6184831798031283 1103 26-27 0.6948308963687708 1103 28-29 0.6286301495384023 1103 30-31 0.6661993939084496 1103 32-33 0.7510141716287109 1103 34-35 0.7650054059794442 1103 36-37 0.8954206024926563 1103 38-39 0.8967915020942527 1103 40-41 0.8840492172787435 1103 42-43 0.9354697759636252 1103 44-45 0.9524432841837367 1103 46-47 0.7931817683462796 1103 48-49 0.9331578489608248 1103 50-51 1.1199807463908726 1103 52-53 1.2057881394126113 1103 54-55 1.293157681102528 1103 56-57 1.3085323640963011 1103 58-59 1.265934119384461 1103 60-61 1.271935273654858 1103 62-63 1.465670763523402 1103 64-65 1.629244015351727 1103 66-67 1.4964931194496351 1103 68-69 1.5690571570020673 1103 70-71 1.6474910252237542 1103 72-73 1.6924129853716927 1103 74-75 1.6382219839146295 1103 76-77 1.6746649945753234 1103 78-79 1.5640302757532538 1103 80-81 1.7410409274752077 1103 82-83 1.5659749816420394 1103 84-85 1.8920173212555582 1103 86-87 1.9051927139488178 1103 88-89 2.0742680693889604 1103 90-91 2.0116236161223746 1103 92-93 1.9422709809709318 1103 94-95 1.941615435779255 1103 96-97 2.0817051906698616 1103 98-99 2.0089899197175534 1103 100-101 1.7496270251273085 1104 1 0.388686085922501 1104 2 0.39266424339134787 1104 3 0.37362208234839045 1104 4 0.2686819034411343 1104 5 0.36239237804670665 1104 6 0.4224400055169326 1104 7 0.30505833061397425 1104 8 0.5109239229862581 1104 9 0.48297364920912145 1104 10-11 0.5208739731588992 1104 12-13 0.5834195204739174 1104 14-15 0.7488332007814691 1104 16-17 0.735718633595404 1104 18-19 0.6695682164552679 1104 20-21 0.7293197228889596 1104 22-23 0.7295435014347689 1104 24-25 0.6805471558669112 1104 26-27 0.7850642663333431 1104 28-29 0.6975239686130905 1104 30-31 0.8598415851739531 1104 32-33 0.7495677253467505 1104 34-35 0.7375319306141463 1104 36-37 0.8521936528245178 1104 38-39 0.7928530209686429 1104 40-41 0.8242724352424347 1104 42-43 0.8167537181994149 1104 44-45 0.7110768743859452 1104 46-47 0.629255091454084 1104 48-49 0.6648562674546667 1104 50-51 0.8155215193925827 1104 52-53 0.8134681843605378 1104 54-55 0.9107871371218579 1104 56-57 0.7980245704659978 1104 58-59 0.9024538153916808 1104 60-61 0.9895851687358466 1104 62-63 1.0739739297321371 1104 64-65 1.0937265086651387 1104 66-67 1.1430017990727457 1104 68-69 1.1283936888534605 1104 70-71 1.1684814463269468 1104 72-73 1.309477170778294 1104 74-75 1.3546820915033315 1104 76-77 1.2714799674935655 1104 78-79 1.5675448939592336 1104 80-81 1.5791649547627067 1104 82-83 1.4855230982995593 1104 84-85 1.5684926816065712 1104 86-87 1.885978330251806 1104 88-89 1.7570212380062635 1104 90-91 1.5563114184595435 1104 92-93 1.893794839073422 1104 94-95 1.8039783443747837 1104 96-97 1.8121368375404998 1104 98-99 1.9554255319462328 1104 100-101 1.645109722381136 1105 1 0.36436034501982917 1105 2 0.1752169776665795 1105 3 0.2381154602738924 1105 4 0.1897238869598894 1105 5 0.16183201399465474 1105 6 0.23284498809515952 1105 7 0.2125766270110958 1105 8 0.34249129824257807 1105 9 0.5378620249818553 1105 10-11 0.5188603285045801 1105 12-13 0.48760001487693216 1105 14-15 0.5028404648235139 1105 16-17 0.6236314427689962 1105 18-19 0.5692688033050501 1105 20-21 0.6156141986197454 1105 22-23 0.652210257388802 1105 24-25 0.7195434972313848 1105 26-27 0.7038465927584951 1105 28-29 0.5220558431282925 1105 30-31 0.6077431389886812 1105 32-33 0.578939318314859 1105 34-35 0.6932808562682524 1105 36-37 0.6801664390667028 1105 38-39 0.6990038470699105 1105 40-41 0.7887752999501814 1105 42-43 0.7567968251077204 1105 44-45 0.8454974959891146 1105 46-47 0.7465196614989438 1105 48-49 0.8206789839625728 1105 50-51 0.6382136020503637 1105 52-53 0.7188867253106039 1105 54-55 0.6032087434616002 1105 56-57 0.652308153138577 1105 58-59 0.8333202419268488 1105 60-61 0.6910419263940604 1105 62-63 0.7831461028105373 1105 64-65 0.9764030433024082 1105 66-67 0.9849163776814684 1105 68-69 1.0120988310757788 1105 70-71 1.0507179831448923 1105 72-73 1.0884124790794019 1105 74-75 1.1224696150539444 1105 76-77 1.2602701108555863 1105 78-79 1.3964457107239703 1105 80-81 1.2825314746632728 1105 82-83 1.3579721089912908 1105 84-85 1.415543172322188 1105 86-87 1.4503872285959964 1105 88-89 1.3580717381657372 1105 90-91 1.4650218728506559 1105 92-93 1.6908830837482682 1105 94-95 1.742477638446573 1105 96-97 1.703119414914653 1105 98-99 1.6752137577762944 1105 100-101 1.631608528806563 1106 1 0.04702037934534431 1106 2 -0.02068515639508206 1106 3 -0.07812929287235804 1106 4 -0.01648368190681282 1106 5 -0.02988549306514443 1106 6 -0.2733556622152946 1106 7 0.07729813059437163 1106 8 0.1037363203498245 1106 9 -0.13323074152381054 1106 10-11 -0.11800157000815403 1106 12-13 0.0011162772055755 1106 14-15 0.1711576892543718 1106 16-17 -0.03508937200349038 1106 18-19 -0.16366625631802378 1106 20-21 0.12923555026543454 1106 22-23 0.09591486176410058 1106 24-25 -0.020930335909277176 1106 26-27 0.02286688797954639 1106 28-29 0.10825864343507163 1106 30-31 -0.172003523442271 1106 32-33 -0.0038142687620279503 1106 34-35 -0.29712560975690394 1106 36-37 -0.1754819446807403 1106 38-39 -0.009287577404997194 1106 40-41 0.25652116835416905 1106 42-43 -0.20276074590727688 1106 44-45 -0.020867925912561702 1106 46-47 0.030912836453161674 1106 48-49 0.2459149337957669 1106 50-51 -0.01818484453922764 1106 52-53 -0.17198362229864017 1106 54-55 0.13008158310435647 1106 56-57 -0.7583294738609041 1106 58-59 -0.272735981407525 1106 60-61 0.2860825902506825 1106 62-63 0.12400268942832682 1106 64-65 0.2510226157457822 1106 66-67 -0.175139980640882 1106 68-69 -0.20141360828535682 1106 70-71 -0.325614304116268 1106 72-73 0.14618064756332672 1106 74-75 0.4656073799170848 1106 76-77 0.3826701546336402 1106 78-79 0.5142911792726146 1106 80-81 0.5441808263319636 1106 82-83 0.19639667084707568 1106 84-85 0.26893203002964583 1106 86-87 0.06292921780440608 1106 88-89 0.1362098362698525 1106 90-91 0.3172382974693484 1106 92-93 0.13830835742030345 1106 94-95 0.17848035884318847 1106 96-97 0.3114452291813592 1106 98-99 0.4816440145004037 1106 100-101 0.19337838007282748 1107 1 0.270757409932429 1107 2 0.04068774131971864 1107 3 0.17479085347908097 1107 4 0.11489835869569731 1107 5 0.15857582710521 1107 6 0.2463555295208124 1107 7 0.17904146041254876 1107 8 0.17484486042016556 1107 9 0.29140461121453853 1107 10-11 0.3711975580415299 1107 12-13 0.3100959351774222 1107 14-15 0.5262777389906361 1107 16-17 0.6034120033438484 1107 18-19 0.5119811990855254 1107 20-21 0.5992914513748175 1107 22-23 0.49803067537183665 1107 24-25 0.4425368066137594 1107 26-27 0.3741924599156121 1107 28-29 0.43033932884829795 1107 30-31 0.4985428089485069 1107 32-33 0.7617742650847603 1107 34-35 0.6332745244996971 1107 36-37 0.6958573530032695 1107 38-39 0.7624521415759702 1107 40-41 0.566002820767217 1107 42-43 0.628278819670193 1107 44-45 0.64428950919919 1107 46-47 0.5627766808172652 1107 48-49 0.560036079830482 1107 50-51 0.5458509232986088 1107 52-53 0.6961299778365273 1107 54-55 0.7094631644422975 1107 56-57 0.748453485466456 1107 58-59 0.7731283378778997 1107 60-61 0.7161949502410359 1107 62-63 0.8970741696530418 1107 64-65 0.916112490364597 1107 66-67 0.9096889661395551 1107 68-69 1.0301699395399702 1107 70-71 1.1281878960480611 1107 72-73 1.1877454774217853 1107 74-75 1.1921118628024665 1107 76-77 1.2800785471717653 1107 78-79 1.4419273676898214 1107 80-81 1.4136338529931187 1107 82-83 1.2649023583234076 1107 84-85 1.3756862527031615 1107 86-87 1.4535047859078283 1107 88-89 1.5431734108486417 1107 90-91 1.5467477748569287 1107 92-93 1.6118012813862492 1107 94-95 1.644075595734659 1107 96-97 1.9114657460431843 1107 98-99 1.8025895857775787 1107 100-101 1.3405816447654626 1108 1 0.2899993725715042 1108 2 0.2833199421783341 1108 3 0.21112443842132222 1108 4 0.1052711784706375 1108 5 0.23713683637341632 1108 6 0.32002380265051045 1108 7 0.2790138892805061 1108 8 0.37477508074046284 1108 9 0.3962253840588872 1108 10-11 0.426166292761728 1108 12-13 0.44398617551659214 1108 14-15 0.4924233963133773 1108 16-17 0.6526130364094556 1108 18-19 0.6001921045680518 1108 20-21 0.5032086157602649 1108 22-23 0.4681235157799364 1108 24-25 0.5866072495363639 1108 26-27 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2316 52-53 -1.6380864962254265 2316 54-55 -1.819996369648603 2316 56-57 -1.805772879351224 2316 58-59 -1.784789492120023 2316 60-61 -1.7276010968802247 2316 62-63 -1.7300007568382405 2316 64-65 -1.7283139779403882 2316 66-67 -1.6984431435449778 2316 68-69 -1.8527476952555482 2316 70-71 -2.153321563610657 2316 72-73 -2.1022094518443666 2316 74-75 -2.1365183688847686 2316 76-77 -2.2635973153300633 2316 78-79 -2.4574586681907853 2316 80-81 -2.7026993479028896 2316 82-83 -2.7316901209183193 2316 84-85 -2.5960343994410486 2316 86-87 -2.620782867960468 2316 88-89 -2.7984185608668284 2316 90-91 -2.544128704964997 2316 92-93 -2.6569086063963923 2316 94-95 -2.9474021074958223 2316 96-97 -3.2844948329812027 2316 98-99 -3.3230584486693395 2316 100-101 -3.009880849795147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11305.0 3 3779.0 4 4093.0 5 4553.0 6 5752.0 7 7048.0 8 8992.0 9 11629.0 10 14700.0 11 18290.0 12 22837.0 13 26988.0 14 30540.0 15 35521.0 16 41060.0 17 47378.0 18 45215.0 19 42131.0 20 44338.0 21 47246.0 22 52315.0 23 58272.0 24 64408.0 25 72263.0 26 80515.0 27 92027.0 28 104651.0 29 120542.0 30 138189.0 31 161519.0 32 188211.0 33 226903.0 34 277086.0 35 337197.0 36 391995.0 37 369052.0 38 163560.0 39 7783.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69958824357896 18.239335934772292 14.545541159664205 23.51553466198455 2 15.53639526152962 19.719802914227408 39.95438652092074 24.789415303322233 3 19.16894976357395 23.530960808342197 29.68215558709285 27.617933840991 4 12.86540630257092 16.224672277391086 36.32863587472825 34.58128554530974 5 13.751657299258607 37.36419332718125 33.91801843610219 14.966130937457955 6 32.8953120039424 35.64640303451021 17.050225313804557 14.408059647742835 7 29.22600987530971 32.75381096748913 19.877967357733155 18.142211799467997 8 27.24884119709327 34.115713965239756 19.494731745487755 19.14071309217921 9 26.815498456380926 16.472984298647674 19.652289149210198 37.059228095761206 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26.469781053448067 28.61098981131014 22.193787904131874 36-37 22.87315449791272 25.76074323920288 28.62271090247176 22.743391360412637 38-39 22.134481873278318 26.240694688101406 28.604771097281343 23.02005234133893 40-41 22.73271068976693 26.03271172569744 28.788286881715976 22.446290702819656 42-43 22.363132384668464 26.186885469575493 28.500893156149388 22.949088989606654 44-45 22.305762075047095 26.431146418781136 28.788464517285874 22.474626988885895 46-47 22.89897773530486 26.841339246202406 27.813284484548113 22.446398533944624 48-49 22.46575597827888 26.912463368099115 27.62933013923673 22.992450514385272 50-51 22.7861461424497 27.12137230725712 27.66154090152276 22.430940648770427 52-53 23.193940595465655 26.571870727968893 27.802837072500992 22.43135160406446 54-55 22.29781242939926 27.024161560974107 28.063064149375666 22.614961860250965 56-57 22.49725577183054 27.108284084689867 27.711385465703547 22.683074677776048 58-59 22.456347916358098 26.52261643058702 27.73010940634764 23.29092624670724 60-61 21.77286347393109 26.93253231331523 28.2210992815975 23.073504931156176 62-63 22.215797858615606 26.877676434592612 28.32372046009227 22.582805246699508 64-65 21.847312981948757 27.053679998378495 27.982835703972704 23.116171315700043 66-67 21.72662098281966 27.04272766802144 28.09140852807556 23.139242821083336 68-69 22.68119712659553 26.408787399109713 28.200322801570838 22.709692672723918 70-71 22.276943994622957 26.74653602104528 27.5725814077414 23.403938576590363 72-73 22.215336342896666 26.9067510374322 27.719812583727432 23.1581000359437 74-75 22.328012450365975 27.306027941701576 27.581254190581934 22.784705417350516 76-77 22.495466210841318 26.821476429031403 27.68159423652411 23.001463123603166 78-79 22.538214486968027 27.222570954016273 27.539417993840587 22.699796565175113 80-81 22.865445331123286 27.175541846643604 27.15280324630875 22.806209575924363 82-83 22.66096395292154 27.311111785074164 27.315180220597973 22.712744041406317 84-85 22.50553413439895 27.501781589614367 27.327461718939205 22.665222557047482 86-87 22.986537436950876 27.51546609414044 26.73994576269782 22.758050706210863 88-89 22.698601609306582 27.575728157063967 26.589594263656107 23.136075969973344 90-91 22.584784991937642 27.519649778729104 26.806631260166743 23.08893396916651 92-93 22.900741331342125 27.02117744968818 27.086761826163812 22.991319392805874 94-95 22.556928688785298 26.785777451773956 27.387606753548067 23.269687105892682 96-97 23.055360814016183 26.26272806774904 27.31415478200615 23.36775633622863 98-99 22.70265863050542 26.70860540166527 26.756193776429043 23.832542191400265 100-101 23.207395521003722 26.071882277821178 27.308239335583924 23.412482865591176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 583.0 1 411.0 2 348.0 3 504.0 4 477.0 5 474.5 6 857.0 7 1822.5 8 2889.0 9 3221.5 10 2751.0 11 2344.0 12 2312.5 13 2385.0 14 2639.0 15 2985.5 16 3343.0 17 3659.5 18 4057.5 19 4648.0 20 5472.5 21 6649.5 22 8043.0 23 9732.5 24 11926.0 25 14910.5 26 19053.0 27 24037.0 28 28339.5 29 32830.0 30 38690.0 31 47729.0 32 61557.0 33 66019.0 34 63176.0 35 66252.0 36 71936.0 37 78570.0 38 84533.0 39 88452.0 40 93508.5 41 100282.5 42 104219.0 43 107080.0 44 109925.0 45 116913.0 46 122322.5 47 124821.5 48 126097.0 49 123182.0 50 121522.0 51 119114.5 52 115727.0 53 116263.0 54 120667.5 55 116667.0 56 106342.0 57 97872.5 58 91202.0 59 80100.0 60 62913.0 61 52131.0 62 45843.5 63 35038.5 64 24279.0 65 18002.5 66 13744.5 67 10578.5 68 8154.5 69 6382.5 70 5032.5 71 3512.5 72 2549.0 73 2088.5 74 1589.5 75 1174.0 76 885.5 77 621.0 78 422.0 79 286.0 80 178.0 81 108.0 82 63.0 83 43.0 84 29.0 85 17.0 86 14.5 87 8.5 88 5.5 89 4.5 90 5.5 91 5.5 92 3.5 93 2.5 94 2.0 95 2.0 96 1.5 97 1.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006390751871957735 2 0.10914575766505596 3 0.11796262830321988 4 0.12092130972542253 5 0.16030135945493987 6 0.09645301436380656 7 0.028166647139369283 8 0.028580862538477653 9 0.024024493148285565 10-11 0.05216155347343281 12-13 0.05412907661919758 14-15 0.028373754838923466 16-17 0.031909379138455644 18-19 0.037116658441532314 20-21 0.05954346362182844 22-23 0.03313723192866974 24-25 0.05106684134721783 26-27 0.05822685038894826 28-29 0.05404031617653151 30-31 0.06799049908221702 32-33 0.050933700683218716 34-35 0.06269445933647427 36-37 0.053433786484979956 38-39 0.03258987586556225 40-41 0.03784153538997197 42-43 0.033595827549111154 44-45 0.007677778290615891 46-47 0.014068530162573626 48-49 0.009349433294160391 50-51 0.008609762938609728 52-53 0.010177864092377134 54-55 0.011967866352809742 56-57 0.015340763174120767 58-59 0.02048886884875339 60-61 0.02541507341672081 62-63 0.011479683918146303 64-65 0.009186705815939245 66-67 0.010429352013264361 68-69 0.012086213609697848 70-71 0.03179103188156753 72-73 0.02924656585847325 74-75 0.030400451613132286 76-77 0.024024493148285565 78-79 0.005251659524409713 80-81 0.004985378196411474 82-83 0.006109677136848483 84-85 0.005251659524409713 86-87 0.014068530162573626 88-89 0.03804864308952615 90-91 0.0360959133508724 92-93 0.030489212055798364 94-95 0.0473536961623535 96-97 0.043625757570378156 98-99 0.026850033906489098 100-101 0.07522447515950252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3379884.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.600481724778504 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31317700035784 38.10883036441615 2 13.123671600816408 13.281282099982105 3 4.766631893909225 7.235816101095001 4 2.264049152188175 4.58247910997192 5 1.2391656717728305 3.1351189964256987 6 0.7437312124125111 2.257989457309597 7 0.49631052372622836 1.7579486109936981 8 0.3617415102277262 1.4643435741897466 9 0.26576406187493107 1.2103010600404815 >10 1.281300240713102 11.463556515965491 >50 0.08803747586733181 3.0429800865647443 >100 0.0469843985812561 4.620154708822108 >500 0.005039951241014211 1.7304265346607313 >1k 0.003926473638219686 4.401950789261121 >5k 4.102285890677284E-4 1.3777521194515165 >10k+ 5.8604084152532636E-5 0.3290698708498198 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11097 0.32832487742182864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8713 0.2577899123165174 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6921 0.20477034123064577 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6652 0.19681148820492061 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6598 0.19521380023693122 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6343 0.18766916261031444 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5856 0.1732603840841875 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5378 0.1591178868860588 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4999 0.14790448429591074 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4985 0.1474902688968024 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4795 0.14186877419461733 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4683 0.13855505100175036 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4168 0.12331784167740668 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3924 0.11609865900723221 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3627 0.10731137518329031 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3617 0.10701550704107005 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3508 0.10379054429086915 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3383 0.10009219251311584 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.917362844405311E-5 2 0.0 0.0 0.0 0.0 5.917362844405311E-5 3 0.0 0.0 0.0 0.0 5.917362844405311E-5 4 0.0 0.0 0.0 0.0 5.917362844405311E-5 5 0.0 0.0 0.0 0.0 5.917362844405311E-5 6 0.0 0.0 0.0 0.0 5.917362844405311E-5 7 0.0 0.0 0.0 0.0 5.917362844405311E-5 8 0.0 0.0 0.0 0.0 5.917362844405311E-5 9 0.0 0.0 0.0 0.0 5.917362844405311E-5 10-11 0.0 0.0 0.0 0.0 5.917362844405311E-5 12-13 0.0 0.0 0.0 0.0 3.9942199199735845E-4 14-15 8.876044266607967E-5 0.0 0.0 0.0 4.733890275524249E-4 16-17 8.876044266607967E-5 0.0 0.0 0.0 5.473560631074912E-4 18-19 8.876044266607967E-5 0.0 0.0 0.0 5.917362844405311E-4 20-21 8.876044266607967E-5 0.0 0.0 1.4793407111013277E-5 5.917362844405311E-4 22-23 8.876044266607967E-5 0.0 0.0 1.7752088533215933E-4 6.065296915515443E-4 24-25 1.4793407111013277E-4 0.0 0.0 5.177692488854647E-4 6.213230986625576E-4 26-27 1.4793407111013277E-4 0.0 0.0 0.0012574396044361285 6.804967271066108E-4 28-29 1.7752088533215933E-4 0.0 0.0 0.003624384742198253 6.952901342176241E-4 30-31 2.071076995541859E-4 0.0 0.0 0.012870264186581552 7.248769484396506E-4 32-33 2.071076995541859E-4 0.0 0.0 0.030681526348241533 7.98843983994717E-4 34-35 2.071076995541859E-4 0.0 0.0 0.05770908114006279 8.284307982167436E-4 36-37 2.3669451377621243E-4 0.0 0.0 0.1041899662828665 8.284307982167436E-4 38-39 2.3669451377621243E-4 0.0 0.0 0.17586402373572585 9.171912408828232E-4 40-41 2.3669451377621243E-4 0.0 0.0 0.25181337584366803 9.171912408828232E-4 42-43 2.6628132799823897E-4 0.0 0.0 0.3322599237133582 9.171912408828232E-4 44-45 2.9586814222026553E-4 0.0 0.0 0.4337870767162423 9.615714622158631E-4 46-47 3.106615493312788E-4 0.0 0.0 0.5564688018878754 0.0010207450906599162 48-49 3.5504177066431866E-4 0.0 0.0 0.6869910328283456 0.001124298940437009 50-51 3.5504177066431866E-4 0.0 0.0 0.832336257694051 0.001124298940437009 52-53 3.5504177066431866E-4 0.0 0.0 0.9736280890113389 0.0012426461973251153 54-55 3.5504177066431866E-4 0.0 0.0 1.1233669557890154 0.0012426461973251153 56-57 3.5504177066431866E-4 0.0 0.0 1.2987575904971886 0.0012574396044361287 58-59 3.5504177066431866E-4 1.4793407111013277E-5 0.0 1.4856279091235085 0.0013018198257691684 60-61 3.5504177066431866E-4 2.9586814222026554E-5 0.0 1.6920403185434765 0.001331406639991195 62-63 3.5504177066431866E-4 2.9586814222026554E-5 0.0 1.8964999982247912 0.001449753896879301 64-65 3.5504177066431866E-4 2.9586814222026554E-5 0.0 2.1154424234677878 0.0014793407111013278 66-67 3.846285848863452E-4 2.9586814222026554E-5 0.0 2.370110926883881 0.001494134118212341 68-69 3.846285848863452E-4 2.9586814222026554E-5 0.0 2.6451203650776183 0.0015089275253233542 70-71 3.846285848863452E-4 2.9586814222026554E-5 0.0 2.9361510631725825 0.0015533077466563941 72-73 3.846285848863452E-4 2.9586814222026554E-5 0.0 3.2465907113971957 0.0016716550035445004 74-75 3.994219919973585E-4 2.9586814222026554E-5 0.0 3.550787541820962 0.001819589074654633 76-77 4.142153991083718E-4 2.9586814222026554E-5 0.0 3.8771301026899145 0.0018935561102096997 78-79 4.142153991083718E-4 2.9586814222026554E-5 0.0 4.2572466984074016 0.0020414901813198324 80-81 5.621494702185045E-4 2.9586814222026554E-5 0.0 4.654035463939 0.002071076995541859 82-83 5.621494702185045E-4 2.9586814222026554E-5 0.0 5.062629368345186 0.0021598374382079386 84-85 5.917362844405311E-4 2.9586814222026554E-5 0.0 5.486238580969051 0.0021598374382079386 86-87 6.509099128845842E-4 2.9586814222026554E-5 0.0 5.921682519281727 0.002219011066651992 88-89 6.804967271066108E-4 2.9586814222026554E-5 0.0 6.376298713210275 0.002278184695096045 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 59.321922 1 GTATCAA 11285 0.0 43.32621 1 ATCAACG 14430 0.0 33.55421 3 TATCAAC 14575 0.0 33.35093 2 TCAACGC 14690 0.0 32.895576 4 CAACGCA 15005 0.0 32.1416 5 AACGCAG 15680 0.0 30.8849 6 ACGCAGA 17930 0.0 26.721218 7 CGCAGAG 17965 0.0 26.536081 8 TACATGG 13215 0.0 25.769821 2 GTACATG 13670 0.0 25.711952 1 AGGTAAG 2210 0.0 25.395414 2 GTGTAAG 1480 0.0 25.387768 1 TAAGGTA 1550 0.0 25.16215 4 TAAGGTG 2605 0.0 24.648563 5 TGGTATC 2645 0.0 24.635448 2 ACATGGG 13620 0.0 24.165432 3 GCAGAGT 19680 0.0 23.522186 9 GTGGTAT 3000 0.0 22.82971 1 GAGTACT 11995 0.0 22.185894 12-13 >>END_MODULE