FastQCFastQC Report
Thu 26 May 2016
SRR938359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379884
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111910.3311060379586992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92500.27367803155374565No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA71930.212817954699037No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA69570.20583546654263873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66730.1974328113035832No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64230.19003610774807655No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC63580.18811296482364484No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56950.16849690699444123No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG54230.16044929352605No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48050.1421646423368376No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46100.13639521356354242No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG43440.12852512098048335No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43290.12808131876715295No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA41370.12240065043652384No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38210.11305121714236346No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36860.10905699722238989No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT36350.10754806969706651No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34660.10254789809354403No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34410.10180822773799338No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG34230.1012756650819969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.056.18591
GTATCAA111950.042.284061
TATCAAC146550.032.1712042
ATCAACG148350.031.7319033
TCAACGC150550.031.26824
CAACGCA153850.030.6592665
AACGCAG160250.029.4491926
TAAGGTG27350.026.5732145
ACGCAGA181850.025.6379457
CGCAGAG181750.025.625938
AGGTAAG25100.025.373042
TGGTATC26550.024.524352
GTAAGGT28150.024.2993184
AAGGTAA25350.024.1853981
GCAGAGT204000.022.3887569
GTACATG129000.022.3635331
TACATGG126000.022.292482
GTGTAAG14100.021.9096621
GAGTACT122200.021.7765712-13
GTGGTAT31450.021.6101041
Produced by FastQC (version 0.11.5)