##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938359_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3379884 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824301070687632 31.0 31.0 34.0 30.0 34.0 2 32.047391567284556 33.0 31.0 34.0 30.0 34.0 3 32.10634004007238 33.0 31.0 34.0 30.0 34.0 4 35.77897910105791 37.0 35.0 37.0 35.0 37.0 5 35.67372963095775 37.0 35.0 37.0 33.0 37.0 6 35.67778568731945 37.0 35.0 37.0 33.0 37.0 7 35.619028345351495 37.0 35.0 37.0 33.0 37.0 8 35.58707221904657 37.0 35.0 37.0 33.0 37.0 9 37.17906945918854 39.0 37.0 39.0 33.0 39.0 10-11 37.081555609600805 39.0 37.0 39.0 33.0 39.0 12-13 36.97327659765838 39.0 37.0 39.0 33.0 39.0 14-15 38.027340435352215 40.0 37.0 41.0 33.0 41.0 16-17 37.94253131172549 40.0 37.0 41.0 33.0 41.0 18-19 38.18691913686979 40.0 37.5 41.0 33.0 41.0 20-21 38.158384873563705 40.0 37.0 41.0 33.0 41.0 22-23 38.05443396873976 40.0 37.0 41.0 33.0 41.0 24-25 37.921998062655405 40.0 37.0 41.0 32.5 41.0 26-27 37.72955699071329 40.0 37.0 41.0 32.0 41.0 28-29 37.61968250981395 40.0 37.0 41.0 32.0 41.0 30-31 37.35019530256068 39.0 36.0 41.0 31.5 41.0 32-33 37.13618825379806 39.0 36.0 41.0 31.0 41.0 34-35 37.024093134557276 39.0 36.0 41.0 31.0 41.0 36-37 36.960665957766594 39.0 36.0 41.0 31.0 41.0 38-39 36.7888434928536 39.0 35.0 41.0 30.0 41.0 40-41 36.604045434695394 39.0 35.0 41.0 30.0 41.0 42-43 36.375432559223924 39.0 35.0 40.0 30.0 41.0 44-45 36.077555620252056 39.0 35.0 40.0 29.5 41.0 46-47 35.683813408980896 38.0 35.0 40.0 28.5 41.0 48-49 35.298136267398526 38.0 34.0 40.0 27.0 41.0 50-51 34.90516715958299 38.0 34.0 40.0 26.0 41.0 52-53 34.40915546214012 38.0 33.0 40.0 24.0 41.0 54-55 34.062867246331535 37.0 33.0 40.0 24.0 41.0 56-57 33.71932897697081 37.0 33.0 40.0 23.0 41.0 58-59 33.23541799067661 36.5 32.0 39.0 22.0 41.0 60-61 32.83129494976751 36.0 31.0 39.0 20.5 40.0 62-63 32.354964105276984 35.5 31.0 39.0 19.5 40.0 64-65 31.835003361062096 35.0 30.5 38.0 18.0 40.0 66-67 31.332404751168976 35.0 30.0 38.0 14.5 40.0 68-69 30.832048969727957 34.0 29.0 37.5 11.5 39.5 70-71 30.253421271262564 34.0 29.0 37.0 8.0 39.0 72-73 29.762345837904498 34.0 28.5 36.0 7.0 39.0 74-75 29.186728449852126 34.0 28.0 36.0 4.0 38.5 76-77 27.966513495729437 31.5 26.0 34.5 2.0 36.5 78-79 28.647261562822866 33.0 27.5 35.0 2.0 37.0 80-81 28.287270657809557 33.0 27.0 35.0 2.0 36.5 82-83 27.85253280881829 33.0 26.0 35.0 2.0 36.0 84-85 27.302161849341573 33.0 26.0 35.0 2.0 36.0 86-87 26.70503617875643 32.0 25.0 35.0 2.0 35.0 88-89 25.9519245335047 32.0 23.5 34.0 2.0 35.0 90-91 25.350602860926585 31.0 21.0 34.0 2.0 35.0 92-93 24.545449340864955 31.0 18.5 34.0 2.0 35.0 94-95 23.742760550362085 31.0 9.5 34.0 2.0 35.0 96-97 22.691082149564895 30.0 2.0 34.0 2.0 35.0 98-99 21.322871435824425 29.5 2.0 34.0 2.0 35.0 100-101 17.349312432024295 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30727880160157284 1101 2 0.3351515345356191 1101 3 0.34327705971222144 1101 4 0.238357050127874 1101 5 0.11440323401729557 1101 6 0.2569682964498483 1101 7 0.2857130861977879 1101 8 0.3492487232366699 1101 9 0.36562507860872984 1101 10-11 0.3320346925738775 1101 12-13 0.3987829673202796 1101 14-15 0.5499606973473732 1101 16-17 0.5118689938864449 1101 18-19 0.48287697123591755 1101 20-21 0.4732882409829955 1101 22-23 0.44896149210264724 1101 24-25 0.44741366849045505 1101 26-27 0.35806734230649084 1101 28-29 0.42928482414935587 1101 30-31 0.5363333227585159 1101 32-33 0.5082387196828719 1101 34-35 0.5580116372593764 1101 36-37 0.5363231611933443 1101 38-39 0.608899083683589 1101 40-41 0.6812328104961836 1101 42-43 0.7292595232407351 1101 44-45 0.7148500278248449 1101 46-47 0.6237201392262577 1101 48-49 0.7595445023689678 1101 50-51 0.9334135939996671 1101 52-53 0.9219355399978255 1101 54-55 0.9104655921128426 1101 56-57 0.7864858819934852 1101 58-59 0.9353868652613002 1101 60-61 0.7808659866204479 1101 62-63 0.9300756001453934 1101 64-65 0.9642108177796658 1101 66-67 0.7306023806534547 1101 68-69 0.9092373062570047 1101 70-71 0.9516210974843347 1101 72-73 0.9222829175767266 1101 74-75 1.0543044906824228 1101 76-77 0.9941695932576593 1101 78-79 1.133096271858026 1101 80-81 1.1796367890713846 1101 82-83 1.1026641703684241 1101 84-85 1.2564592486311525 1101 86-87 1.1706075823000255 1101 88-89 1.3400778829973206 1101 90-91 1.2034383286592742 1101 92-93 1.4904423639219004 1101 94-95 1.292709874085073 1101 96-97 1.4115566172870793 1101 98-99 1.5213649610757187 1101 100-101 1.7393475850428501 1102 1 0.23060572737398033 1102 2 0.2793548945003792 1102 3 0.22415689792130422 1102 4 0.20307581827428578 1102 5 0.11074912216530919 1102 6 0.12855156470639173 1102 7 0.11747116072573505 1102 8 0.17041830334119368 1102 9 0.22149860937503263 1102 10-11 0.3042032514733748 1102 12-13 0.27867694221065165 1102 14-15 0.36449614918053896 1102 16-17 0.40281631172148735 1102 18-19 0.329619827806674 1102 20-21 0.3288159636957886 1102 22-23 0.33123747776572543 1102 24-25 0.2901039430143939 1102 26-27 0.23775166981294404 1102 28-29 0.27136427790478734 1102 30-31 0.34438862801872006 1102 32-33 0.38282983720154107 1102 34-35 0.2893362002436888 1102 36-37 0.4646985441452216 1102 38-39 0.5195415417841431 1102 40-41 0.4144603189561735 1102 42-43 0.25782723313840705 1102 44-45 0.5820702476842783 1102 46-47 0.5005534024768963 1102 48-49 0.47242390005824575 1102 50-51 0.5977209767112726 1102 52-53 0.4715917343071112 1102 54-55 0.31436919836822597 1102 56-57 0.41019265598679056 1102 58-59 0.4965133945658735 1102 60-61 0.39237977288824766 1102 62-63 0.45960764796181763 1102 64-65 0.5716548016806726 1102 66-67 0.540956498512525 1102 68-69 0.7213155585947639 1102 70-71 0.6621076716418415 1102 72-73 0.6947555352655961 1102 74-75 0.7039757027126221 1102 76-77 0.5906809766901091 1102 78-79 0.8514833686322199 1102 80-81 0.9627246327268963 1102 82-83 0.9372372666220024 1102 84-85 0.8633410691793983 1102 86-87 0.8049491052971618 1102 88-89 0.8029677930912271 1102 90-91 0.8447560836101147 1102 92-93 1.1791320177133713 1102 94-95 1.320286414402645 1102 96-97 1.3522739637559162 1102 98-99 1.584262780502641 1102 100-101 1.4871090435639047 1103 1 0.2773240277816278 1103 2 0.29830851853931506 1103 3 0.3204812952422955 1103 4 0.2212981282806794 1103 5 0.10660869851965771 1103 6 0.17185498520524334 1103 7 0.1975360945253044 1103 8 0.2194410328197023 1103 9 0.2998040759639764 1103 10-11 0.27773348621468585 1103 12-13 0.3811224881194093 1103 14-15 0.5366486170414078 1103 16-17 0.5173949448651953 1103 18-19 0.4700527437678943 1103 20-21 0.4524213730925908 1103 22-23 0.42116602258607827 1103 24-25 0.43628601268135014 1103 26-27 0.5453417275745522 1103 28-29 0.5192391063803825 1103 30-31 0.47328104719343855 1103 32-33 0.4913875924694935 1103 34-35 0.5626441745463922 1103 36-37 0.6659737889523853 1103 38-39 0.5099033506753585 1103 40-41 0.5345664223965727 1103 42-43 0.6536157875582589 1103 44-45 0.7663217958735373 1103 46-47 0.7689112198370154 1103 48-49 0.8048823714293434 1103 50-51 1.0125955408884053 1103 52-53 0.9753218993886463 1103 54-55 0.9054499104264977 1103 56-57 0.9892893152504598 1103 58-59 1.0077868023379182 1103 60-61 0.9468835265128916 1103 62-63 0.8728036236630103 1103 64-65 0.8293956060523229 1103 66-67 0.8578874825598355 1103 68-69 0.936604061081951 1103 70-71 1.0020567435796437 1103 72-73 0.8511146565233467 1103 74-75 0.9085811175959329 1103 76-77 0.8258212828487537 1103 78-79 1.0727573704609306 1103 80-81 1.0140203857325112 1103 82-83 1.2338517017072164 1103 84-85 1.2949972140429544 1103 86-87 1.3558471335776652 1103 88-89 1.368571703724676 1103 90-91 1.49500020495584 1103 92-93 1.8550400967138287 1103 94-95 1.6649527976626572 1103 96-97 1.6551159668166306 1103 98-99 1.8442457704277047 1103 100-101 1.9078475997905144 1104 1 0.22225426260123626 1104 2 0.21355340235855635 1104 3 0.23031856159433772 1104 4 0.1387258498610393 1104 5 0.12206369049035004 1104 6 0.07171456795125408 1104 7 0.18020539095788024 1104 8 0.20269180705045642 1104 9 0.2107082253185979 1104 10-11 0.2652071326558385 1104 12-13 0.2974771589627281 1104 14-15 0.4728012653180187 1104 16-17 0.4844401093388697 1104 18-19 0.34518673442265424 1104 20-21 0.3119821943827077 1104 22-23 0.34648448252528397 1104 24-25 0.32438584174056473 1104 26-27 0.3695699391521572 1104 28-29 0.4090189452719528 1104 30-31 0.40733108327692946 1104 32-33 0.49729551674771955 1104 34-35 0.5117477596208388 1104 36-37 0.6295339583534911 1104 38-39 0.4423038104191832 1104 40-41 0.5133267257770768 1104 42-43 0.5670924674442901 1104 44-45 0.6353801178805938 1104 46-47 0.5827333378730444 1104 48-49 0.5594168042701426 1104 50-51 0.6688581549183326 1104 52-53 0.6005775564842253 1104 54-55 0.5994516310893516 1104 56-57 0.5683737173201138 1104 58-59 0.7412423948748668 1104 60-61 0.8725403564684555 1104 62-63 0.7495004822152467 1104 64-65 0.7118198138248388 1104 66-67 0.6980210207639992 1104 68-69 0.8313795848103922 1104 70-71 0.8945152700673624 1104 72-73 0.7464733688084415 1104 74-75 0.8710867844833494 1104 76-77 0.845940927893384 1104 78-79 1.1717197054121335 1104 80-81 0.9937469525058908 1104 82-83 1.1099999020118219 1104 84-85 1.2570546171926438 1104 86-87 1.188142246616028 1104 88-89 1.1683618206730237 1104 90-91 1.1588512032959528 1104 92-93 1.5110408296387732 1104 94-95 1.4571545303008264 1104 96-97 1.4878612344310298 1104 98-99 1.7861388687328414 1104 100-101 2.0405714379041413 1105 1 0.10715383792821953 1105 2 0.18242267664547285 1105 3 0.14334099461486005 1105 4 0.12691270255449183 1105 5 0.04335121425559407 1105 6 0.10010691894331103 1105 7 0.15067385341662032 1105 8 0.14379391358831128 1105 9 0.18070209110509694 1105 10-11 0.18180801428268722 1105 12-13 0.20720639074369984 1105 14-15 0.37093279848076577 1105 16-17 0.2392883487251538 1105 18-19 0.28342477710630476 1105 20-21 0.17494764231980753 1105 22-23 0.2088408876272041 1105 24-25 0.22496234620042088 1105 26-27 0.0907947471306656 1105 28-29 0.1691976401912072 1105 30-31 0.4144472431304962 1105 32-33 0.37214601407404047 1105 34-35 0.3342627270588494 1105 36-37 0.44644957793266116 1105 38-39 0.4186622339306112 1105 40-41 0.3353638770466034 1105 42-43 0.42780645727153654 1105 44-45 0.4325469182636752 1105 46-47 0.5545338561690087 1105 48-49 0.6641245663038688 1105 50-51 0.6984310307913049 1105 52-53 0.5613949994572849 1105 54-55 0.6429125552049726 1105 56-57 0.6916122987328066 1105 58-59 0.6552270280046812 1105 60-61 0.659566945643995 1105 62-63 0.6236559546934899 1105 64-65 0.5986513873815298 1105 66-67 0.6515977308423508 1105 68-69 0.7909577363645326 1105 70-71 0.7460587609542593 1105 72-73 0.8670823944729769 1105 74-75 0.7156616542976515 1105 76-77 0.6490300989246158 1105 78-79 0.8060024037964162 1105 80-81 0.8491194975863472 1105 82-83 0.9126510927746985 1105 84-85 1.0831913032664353 1105 86-87 1.092258265241032 1105 88-89 1.1115643694838084 1105 90-91 1.2365827634166138 1105 92-93 1.332668460986703 1105 94-95 1.28928063839777 1105 96-97 1.4653534786645892 1105 98-99 1.611870628032996 1105 100-101 1.5561028887336334 1106 1 -0.07395857118749305 1106 2 0.002036885093282592 1106 3 0.04301719264999093 1106 4 0.026957072659698156 1106 5 -0.039707322143286206 1106 6 -0.07803076472405479 1106 7 -0.07958339508201817 1106 8 0.01215645540835908 1106 9 -0.23581808485967315 1106 10-11 0.019662091274547322 1106 12-13 -0.05327437617741282 1106 14-15 -0.0727406808493285 1106 16-17 -0.004318245589317371 1106 18-19 -0.08985332280440872 1106 20-21 0.07677654320988836 1106 22-23 0.06589340843446223 1106 24-25 0.06963868632815462 1106 26-27 0.00890327305867089 1106 28-29 -9.130803908092844E-4 1106 30-31 -0.010064964822696254 1106 32-33 -0.023924711163196832 1106 34-35 -0.0772912280378577 1106 36-37 -0.07882273238132598 1106 38-39 -0.12118822714295874 1106 40-41 -0.06036713692954976 1106 42-43 -0.027488758707519878 1106 44-45 -0.22050973033355348 1106 46-47 -0.7952199261160331 1106 48-49 -0.3853758851784477 1106 50-51 -6.16974177880536E-4 1106 52-53 -0.0979827821321777 1106 54-55 0.06756499501945257 1106 56-57 0.00915702999002832 1106 58-59 -0.18513286886315683 1106 60-61 -0.16769235203289412 1106 62-63 -0.3450580886900809 1106 64-65 -0.09815201972831034 1106 66-67 -0.21057997692663122 1106 68-69 -0.4439960108033425 1106 70-71 -0.3656724546580641 1106 72-73 -0.12034748536743223 1106 74-75 -0.07723462081256116 1106 76-77 -0.0063326787014865715 1106 78-79 0.2523855805064912 1106 80-81 -0.3746232282030171 1106 82-83 0.18619190329117785 1106 84-85 0.3480244594914943 1106 86-87 0.5040682830398175 1106 88-89 0.6056958183309789 1106 90-91 0.5446387117003191 1106 92-93 0.6178420034126795 1106 94-95 0.5799127356269977 1106 96-97 0.6727449833541534 1106 98-99 0.6631059201804526 1106 100-101 0.8768253348644706 1107 1 0.10792079945043298 1107 2 0.15620324324593327 1107 3 0.11001012852158709 1107 4 0.0017956206138691755 1107 5 -0.006645492019636379 1107 6 0.09133013992276773 1107 7 0.07468505810643222 1107 8 0.13523169488319553 1107 9 0.20031013541510134 1107 10-11 0.15337282831263366 1107 12-13 0.16439468651513778 1107 14-15 0.24353832368166906 1107 16-17 0.21693637518959719 1107 18-19 0.2326091181280674 1107 20-21 0.2540697917923822 1107 22-23 0.23794959756274636 1107 24-25 0.21972772592047107 1107 26-27 0.2397174385654992 1107 28-29 0.16516602336482578 1107 30-31 0.1346134048272205 1107 32-33 0.22390132509325866 1107 34-35 0.28364473228011633 1107 36-37 0.2828443246167325 1107 38-39 0.2635796664798917 1107 40-41 0.26571780222604957 1107 42-43 0.22463909102425106 1107 44-45 0.32047286425684973 1107 46-47 0.3529654324772835 1107 48-49 0.4558418297128526 1107 50-51 0.46836126163947256 1107 52-53 0.45523772096476023 1107 54-55 0.2892257942115606 1107 56-57 0.28495983783184187 1107 58-59 0.34175017295569177 1107 60-61 0.4004051183913475 1107 62-63 0.5233581677752568 1107 64-65 0.45296424107525013 1107 66-67 0.3628699806859892 1107 68-69 0.5891105878304117 1107 70-71 0.7404987988373861 1107 72-73 0.8027260253416166 1107 74-75 0.6348336892471274 1107 76-77 0.7006575857377051 1107 78-79 0.9498302445078153 1107 80-81 0.9720021394451202 1107 82-83 1.0654985687270475 1107 84-85 1.0879970879102494 1107 86-87 1.2312697357902032 1107 88-89 1.3316280145061015 1107 90-91 1.2287951196642553 1107 92-93 1.498815541553654 1107 94-95 1.5444321420014475 1107 96-97 1.6881641878544222 1107 98-99 1.5848775560919393 1107 100-101 1.6161310429555193 1108 1 0.147618510422582 1108 2 0.14279641035634683 1108 3 0.1296487411281504 1108 4 0.13392085258291075 1108 5 0.11820854375180545 1108 6 0.1424721502894144 1108 7 0.13309818468480472 1108 8 0.18181794961405728 1108 9 0.15050660872577026 1108 10-11 0.15655938569405947 1108 12-13 0.17601819426861454 1108 14-15 0.2622344626913602 1108 16-17 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-0.8961498951351778 2316 64-65 -0.6919164857503972 2316 66-67 -0.8731451559091461 2316 68-69 -0.8662441444915316 2316 70-71 -0.6619872764741039 2316 72-73 -0.7988372981685288 2316 74-75 -0.7744800406594159 2316 76-77 -0.7362050221003607 2316 78-79 -0.9766248843152603 2316 80-81 -1.0326874857272195 2316 82-83 -1.2614320506321661 2316 84-85 -1.4101000640265937 2316 86-87 -1.383918227609044 2316 88-89 -1.283273601980035 2316 90-91 -1.094861467350821 2316 92-93 -1.4164033989657696 2316 94-95 -1.2497096536264394 2316 96-97 -1.3827862931993842 2316 98-99 -1.5365719749267726 2316 100-101 -1.9200587697612868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 19.0 4 46.0 5 120.0 6 271.0 7 646.0 8 1529.0 9 2884.0 10 4728.0 11 7187.0 12 10413.0 13 14110.0 14 18286.0 15 24521.0 16 31061.0 17 38772.0 18 36681.0 19 32255.0 20 31222.0 21 33030.0 22 35240.0 23 39955.0 24 46058.0 25 53225.0 26 63106.0 27 75550.0 28 89815.0 29 106329.0 30 126751.0 31 150423.0 32 180507.0 33 222469.0 34 283291.0 35 369646.0 36 478930.0 37 519521.0 38 241448.0 39 9839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47048408933829 19.042851326914157 15.181462493254184 23.305202090493367 2 15.975924985228417 20.47394577082793 38.81391595532174 24.736213288621915 3 19.209446241350296 23.686907598012237 30.110471246942204 26.993174913695263 4 13.36868761433458 16.874311921448168 35.95562331595502 33.80137714826223 5 13.704873485915911 37.49049812330535 34.082580484743715 14.722047906035025 6 33.000274565635976 36.062835292572174 16.649654248489 14.287235893302846 7 29.17546874389772 32.82991368934555 19.83961579746524 18.15500176929149 8 27.323689215369523 34.447069781093084 19.312319594400282 18.916921409137117 9 26.667956651766744 16.365857526471324 19.57567182779054 37.39051399397139 10-11 25.19984413666268 26.39037316073569 27.020572303664856 21.38921039893677 12-13 26.364839740062084 23.588102431917783 27.28203098094491 22.765026847075227 14-15 22.48698476042373 25.202018767508 25.62954823301628 26.681448239051992 16-17 22.18857215218037 28.405856532354363 26.835388433449197 22.57018288201607 18-19 22.417559300851746 26.4681568953254 29.173915436151066 21.940368367671788 20-21 22.6329513083881 25.67611787860175 29.62909377954983 22.061837033460318 22-23 22.46770895095808 25.73801349395423 29.590039184776757 22.204238370310932 24-25 22.398275799320224 26.01545188219027 29.546317329027566 22.039954989461936 26-27 21.755339820762565 26.387570811864602 29.196715740800876 22.660373626571957 28-29 22.057702927662678 26.979089941197625 28.947857771894597 22.0153493592451 30-31 22.09067726127297 26.294908653770765 28.89110201598718 22.723312068969086 32-33 21.590695257921315 26.321368171774758 28.978557742491716 23.10937882781221 34-35 22.71300950338654 26.469475550894746 28.649589382033536 22.167925563685177 36-37 22.890491434179058 25.804992440225398 28.727321046122466 22.57719507947307 38-39 22.147282148391703 26.289822804285834 28.706667307742094 22.85622773958037 40-41 22.72660096445237 26.00796397350116 28.893021885171972 22.372413176874506 42-43 22.4132817036322 26.237474434791615 28.501636543177 22.84760731839918 44-45 22.32380761317877 26.405948419141158 28.890970997343757 22.379272970336316 46-47 22.94482591710248 26.81756237788042 27.884684799833366 22.35292690518373 48-49 22.492768982604137 26.93752211614363 27.694826212970625 22.87488268828161 50-51 22.791887964822287 27.056035045165977 27.750884872097036 22.401192117914697 52-53 23.259703587460397 26.531886893159644 27.84049689279277 22.367912626587184 54-55 22.38262138473155 27.040530416896463 28.032990988901226 22.543857209470765 56-57 22.564309899393 27.076742870465374 27.69827307682749 22.660674153314137 58-59 22.43026028515137 26.602398728057096 27.716576911040203 23.25076407575133 60-61 21.83801278387069 26.955821560739956 28.193615520532656 23.0125501348567 62-63 22.19992132052506 26.95767715938393 28.23174315871017 22.610658361380835 64-65 21.847567772628533 27.059056605839405 27.907015281928395 23.18636033960367 66-67 21.691428628915574 27.10085612516814 27.989381136367538 23.218334109548753 68-69 22.651654458002174 26.48127094002475 28.065743909822583 22.80133069215049 70-71 22.2399381517144 26.781144971416914 27.50294499795629 23.475971878912393 72-73 22.165048957330463 26.895628308917292 27.614425592372747 23.32489714137949 74-75 22.17429042643515 27.297167565276865 27.437959200463318 23.09058280782467 76-77 22.247725533977235 26.752506985577128 27.81182522521909 23.187942255226545 78-79 22.308320885660667 27.085903887491785 27.67809445393661 22.927680772910943 80-81 22.583822300141893 27.08275592756805 27.330952238968077 23.002469533321978 82-83 22.391455714701138 27.18147060978073 27.48330870551917 22.943764969998956 84-85 22.165111970387038 27.37553675564779 27.446127650709563 23.01322362325561 86-87 22.596166080488402 27.330731778910483 26.919045852100805 23.154056288500314 88-89 22.222482146415825 27.440419205739442 26.716444781885272 23.62065386595946 90-91 22.04211981431804 27.336032649862602 27.091256342291448 23.530591193527915 92-93 22.2482141041859 26.932153789537967 27.2597462035518 23.559885902724336 94-95 21.885294442321822 26.577170343305177 27.61901858774346 23.91851662662954 96-97 22.203751206317826 26.057811796807904 27.655989603874897 24.082447392999367 98-99 21.84252173161383 26.528776552473808 27.104215832432416 24.524485883479947 100-101 21.753241613203862 26.139097634784186 27.821627937359413 24.286032814652533 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 642.0 1 455.0 2 376.5 3 501.0 4 449.5 5 451.0 6 883.5 7 1936.5 8 2931.5 9 3182.0 10 2754.5 11 2287.5 12 2231.5 13 2369.0 14 2631.5 15 2958.0 16 3288.5 17 3640.0 18 3993.0 19 4552.0 20 5315.5 21 6293.0 22 7667.0 23 9373.0 24 11492.0 25 14435.0 26 18378.0 27 23277.5 28 27876.5 29 32623.5 30 38467.5 31 47485.5 32 62915.0 33 67251.5 34 62972.5 35 66097.5 36 72123.5 37 78962.5 38 84489.0 39 88489.0 40 93959.5 41 101509.5 42 105287.0 43 107102.5 44 110958.0 45 118642.0 46 123247.0 47 125547.0 48 126561.5 49 123808.5 50 122748.0 51 120336.0 52 116938.0 53 119065.5 54 124257.0 55 118470.0 56 107307.5 57 97388.5 58 89593.0 59 78712.0 60 61494.0 61 51024.5 62 44959.5 63 33364.5 64 21861.5 65 16102.5 66 12292.5 67 9688.5 68 7459.5 69 5768.0 70 4573.0 71 3134.5 72 2194.0 73 1782.0 74 1407.5 75 1048.0 76 771.5 77 521.0 78 335.5 79 204.5 80 121.0 81 71.0 82 40.0 83 23.0 84 13.5 85 6.0 86 3.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5504177066431866E-4 2 0.052546182058319166 3 0.0 4 2.9586814222026557E-5 5 0.047457250012130595 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006834554085288135 26-27 0.0019379363315427394 28-29 1.0355384977709295E-4 30-31 0.0015089275253233544 32-33 4.733890275524249E-4 34-35 0.011967866352809742 36-37 0.02411325359095164 38-39 0.0043344682835268905 40-41 0.0011390923475480222 42-43 0.0013757868613242347 44-45 0.008787283823941887 46-47 0.0 48-49 0.0 50-51 5.029758417744514E-4 52-53 0.0 54-55 0.001538514339545381 56-57 0.0 58-59 0.023713831598954283 60-61 0.0 62-63 0.01034059157059828 64-65 0.01576977198034015 66-67 0.007041661784842321 68-69 0.017456220390995668 70-71 1.4793407111013279E-5 72-73 5.9173628444053115E-5 74-75 0.022988954650514634 76-77 0.04951353360056143 78-79 0.03174665166023449 80-81 0.02679086027804505 82-83 0.03702789799886624 84-85 0.016361508264780684 86-87 0.01082877400526172 88-89 0.08376027106255718 90-91 0.026051189922494382 92-93 0.02477895691094724 94-95 0.027722844926038884 96-97 0.038980627737519986 98-99 0.05491312719608128 100-101 0.01507448184612253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3379884.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.83167856504706 #Duplication Level Percentage of deduplicated Percentage of total 1 72.74518672584163 34.79524388624785 2 14.522215389059543 13.892438770837517 3 5.181480373010161 7.435167110787664 4 2.467464070532305 4.720917931700153 5 1.3693520341934347 3.2749203170966847 6 0.8576160925006953 2.461273036122295 7 0.5698456477380969 1.9079671698009781 8 0.39060043887603424 1.4946459711747824 9 0.2811175100552573 1.2101690141972508 >10 1.453950363894694 12.302821559908967 >50 0.09804281849143488 3.2420100743473306 >100 0.052428962757555916 4.925700674185734 >500 0.005937335334982463 1.9394927496570968 >1k 0.0042056125267130534 4.469542432301115 >5k 4.947779443191828E-4 1.5966307104923374 >10k+ 6.184724303989785E-5 0.331058591142069 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11191 0.3311060379586992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9250 0.27367803155374565 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7193 0.212817954699037 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6957 0.20583546654263873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6673 0.1974328113035832 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6423 0.19003610774807655 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6358 0.18811296482364484 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5695 0.16849690699444123 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5423 0.16044929352605 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4805 0.1421646423368376 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4610 0.13639521356354242 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4344 0.12852512098048335 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4329 0.12808131876715295 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4137 0.12240065043652384 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3821 0.11305121714236346 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3686 0.10905699722238989 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3635 0.10754806969706651 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3466 0.10254789809354403 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3441 0.10180822773799338 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3423 0.1012756650819969 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.9586814222026554E-5 0.0 0.0 0.0 0.0 8 2.9586814222026554E-5 0.0 0.0 0.0 2.9586814222026554E-5 9 2.9586814222026554E-5 0.0 0.0 0.0 2.9586814222026554E-5 10-11 2.9586814222026554E-5 0.0 0.0 0.0 2.9586814222026554E-5 12-13 2.9586814222026554E-5 0.0 0.0 0.0 1.923142924431726E-4 14-15 5.917362844405311E-5 0.0 0.0 0.0 2.514879208872257E-4 16-17 7.396703555506638E-5 0.0 0.0 0.0 2.6628132799823897E-4 18-19 8.876044266607967E-5 0.0 0.0 0.0 2.9586814222026553E-4 20-21 8.876044266607967E-5 0.0 0.0 1.4793407111013277E-5 3.846285848863452E-4 22-23 8.876044266607967E-5 0.0 0.0 1.7752088533215933E-4 4.438022133303983E-4 24-25 2.071076995541859E-4 0.0 0.0 5.473560631074913E-4 4.7338902755242486E-4 26-27 2.071076995541859E-4 0.0 0.0 0.0013166132328801816 4.8818243466343815E-4 28-29 3.254549564422921E-4 0.0 0.0 0.0037723188133083857 5.029758417744515E-4 30-31 3.846285848863452E-4 0.0 0.0 0.013003404850580672 5.177692488854647E-4 32-33 3.846285848863452E-4 0.0 0.0 0.03096260108335079 5.621494702185045E-4 34-35 4.29008806219385E-4 0.0 0.0 0.05801974268939407 5.621494702185045E-4 36-37 4.438022133303983E-4 0.0 0.0 0.10470773553175197 5.621494702185045E-4 38-39 4.7338902755242486E-4 0.0 0.0 0.17691435564060778 5.917362844405311E-4 40-41 5.029758417744514E-4 0.0 0.0 0.25329271655476937 6.213230986625576E-4 42-43 5.473560631074912E-4 0.0 0.0 0.33369488420312654 6.213230986625576E-4 44-45 5.917362844405311E-4 0.0 0.0 0.43542914490556484 6.213230986625576E-4 46-47 6.509099128845842E-4 0.0 0.0 0.5584954986620843 6.36116505773571E-4 48-49 6.509099128845842E-4 0.0 0.0 0.6890916966381095 7.100835413286373E-4 50-51 6.509099128845842E-4 0.0 0.0 0.8342446072113717 9.023978337718099E-4 52-53 6.509099128845842E-4 0.0 0.0 0.9754328846788825 9.763648693268763E-4 54-55 6.509099128845842E-4 0.0 0.0 1.1256303470770002 0.0010651253119929559 56-57 6.509099128845842E-4 0.0 0.0 1.3015979246625031 0.001079918719103969 58-59 6.509099128845842E-4 0.0 0.0 1.4882167553679357 0.0010947121262149825 60-61 6.509099128845842E-4 0.0 0.0 1.6958126373567852 0.0010947121262149825 62-63 6.509099128845842E-4 0.0 0.0 1.9010119873936502 0.001124298940437009 64-65 6.509099128845842E-4 0.0 0.0 2.120531355513976 0.001124298940437009 66-67 6.509099128845842E-4 0.0 0.0 2.375614074329178 0.0011390923475480224 68-69 6.804967271066108E-4 0.0 0.0 2.6526354158900127 0.0011538857546590357 70-71 6.804967271066108E-4 0.0 0.0 2.94736446576273 0.001227852790214102 72-73 6.804967271066108E-4 0.0 0.0 3.2619610613855388 0.0012870264186581552 74-75 6.804967271066108E-4 0.0 0.0 3.570817815049274 0.001375786861324235 76-77 6.804967271066108E-4 0.0 0.0 3.9022049277430826 0.001449753896879301 78-79 6.804967271066108E-4 0.0 0.0 4.2890229368818575 0.0014645473039903143 80-81 7.100835413286373E-4 0.0 0.0 4.693681794996515 0.001494134118212341 82-83 7.100835413286373E-4 0.0 0.0 5.111344057961753 0.0015681011537674074 84-85 7.100835413286373E-4 0.0 0.0 5.545382622598883 0.0015681011537674074 86-87 7.248769484396506E-4 0.0 0.0 5.993519304212807 0.0016272747822114605 88-89 7.396703555506639E-4 0.0 0.0 6.4651479163190215 0.0016864484106555136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6175 0.0 56.1859 1 GTATCAA 11195 0.0 42.28406 1 TATCAAC 14655 0.0 32.171204 2 ATCAACG 14835 0.0 31.731903 3 TCAACGC 15055 0.0 31.2682 4 CAACGCA 15385 0.0 30.659266 5 AACGCAG 16025 0.0 29.449192 6 TAAGGTG 2735 0.0 26.573214 5 ACGCAGA 18185 0.0 25.637945 7 CGCAGAG 18175 0.0 25.62593 8 AGGTAAG 2510 0.0 25.37304 2 TGGTATC 2655 0.0 24.52435 2 GTAAGGT 2815 0.0 24.299318 4 AAGGTAA 2535 0.0 24.185398 1 GCAGAGT 20400 0.0 22.388756 9 GTACATG 12900 0.0 22.363533 1 TACATGG 12600 0.0 22.29248 2 GTGTAAG 1410 0.0 21.909662 1 GAGTACT 12220 0.0 21.77657 12-13 GTGGTAT 3145 0.0 21.610104 1 >>END_MODULE