FastQCFastQC Report
Thu 26 May 2016
SRR938358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333045
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112380.337169165132784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84400.25322190369466957No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67820.20347760081247027No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65920.19777710771981777No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65550.19666701169651174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61550.18466597360671697No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57670.17302496665961606No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53530.16060389223667848No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49870.14962294238451626No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49580.14875286712300614No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48100.14431248302978208No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA46020.1380719432230888No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42500.1275110297040694No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39170.11752016549431525No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA36110.10833937135562226No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35810.10743929349888764No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG34050.10215883673937796No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33900.10170879781101064No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33720.10116875109696989No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33430.10029867583545976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61350.053.06971
GTATCAA114450.042.4634251
TATCAAC143600.033.5595632
ATCAACG146350.032.8959923
TCAACGC147300.032.651574
CAACGCA149800.031.9465795
AACGCAG155350.030.8289136
GTGTAAG13950.029.0034581
ACGCAGA173250.027.3387537
TAAGGTG27500.027.3043045
CGCAGAG173700.027.1858738
AGGTAAG24600.026.4678252
TAAGGTA14600.025.7158284
GTACATG128700.024.9649281
TACATGG127200.024.7345312
GCAGAGT190900.024.0381979
AAGGTAA26350.023.8450581
ACATGGG131350.023.1204933
GGTAAGG29900.022.8885123
GTAAGGT30400.022.6683924
Produced by FastQC (version 0.11.5)