##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3333045 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.104842268856256 31.0 31.0 34.0 28.0 34.0 2 31.200210318192525 31.0 31.0 34.0 28.0 34.0 3 31.262757028482962 31.0 31.0 34.0 27.0 34.0 4 34.899231783549276 37.0 35.0 37.0 32.0 37.0 5 34.923096147816786 37.0 35.0 37.0 32.0 37.0 6 34.95484729429096 37.0 35.0 37.0 32.0 37.0 7 34.882413228744284 37.0 35.0 37.0 32.0 37.0 8 34.834559989439086 37.0 35.0 37.0 32.0 37.0 9 36.34841653803054 39.0 35.0 39.0 32.0 39.0 10-11 36.20768501475378 39.0 35.0 39.0 32.0 39.0 12-13 36.07522910731778 39.0 35.0 39.0 30.0 39.0 14-15 37.07267453634739 39.0 36.0 41.0 31.0 41.0 16-17 36.93335628531868 39.0 36.0 41.0 31.0 41.0 18-19 36.937969634373374 39.0 36.0 41.0 31.0 41.0 20-21 37.0116818104766 39.0 36.0 41.0 31.0 41.0 22-23 36.946019780711026 39.0 36.0 41.0 30.5 41.0 24-25 36.82883084386799 39.0 36.0 41.0 30.0 41.0 26-27 36.64036324142038 39.0 36.0 41.0 30.0 41.0 28-29 36.4613953606987 39.0 36.0 41.0 30.0 41.0 30-31 36.222499396197776 39.0 35.0 40.0 29.0 41.0 32-33 35.929030061100285 38.5 35.0 40.0 28.5 41.0 34-35 35.67982835515272 38.0 35.0 40.0 27.0 41.0 36-37 35.38542173898042 38.0 34.0 40.0 26.5 41.0 38-39 35.117798139539076 38.0 34.0 40.0 25.5 41.0 40-41 35.03469650124736 38.0 34.0 40.0 25.0 41.0 42-43 34.84420702390757 38.0 34.0 40.0 24.5 41.0 44-45 34.55502010923945 38.0 33.0 40.0 24.0 41.0 46-47 34.179290408620346 38.0 33.0 40.0 23.0 41.0 48-49 33.79479905011783 38.0 33.0 40.0 21.5 41.0 50-51 33.026160612893015 37.0 32.0 39.5 19.5 40.5 52-53 33.06211977336039 37.0 32.0 39.5 19.5 40.5 54-55 33.186789557296706 37.0 32.0 40.0 18.5 41.0 56-57 32.886719951275786 37.0 32.0 40.0 16.0 41.0 58-59 32.557541227316165 36.5 31.0 39.5 13.5 41.0 60-61 32.11928161785995 36.0 31.0 39.0 9.5 41.0 62-63 31.606996155167423 35.5 30.5 39.0 8.0 40.0 64-65 31.122541699857038 35.0 30.0 38.5 6.5 40.0 66-67 30.601621640271883 35.0 29.0 38.0 2.0 40.0 68-69 30.05711039004874 34.5 28.5 37.5 2.0 40.0 70-71 29.43291794740245 34.0 28.0 37.0 2.0 39.0 72-73 28.90684689225618 34.0 27.0 36.0 2.0 39.0 74-75 28.33866614462151 34.0 26.0 36.0 2.0 39.0 76-77 27.63474675559436 33.0 25.5 35.0 2.0 37.5 78-79 26.884081523051744 32.5 24.5 35.0 2.0 37.0 80-81 26.264402670831025 32.0 22.5 35.0 2.0 36.5 82-83 25.62519677952143 31.5 20.5 35.0 2.0 36.0 84-85 25.00023567038549 31.0 18.5 34.0 2.0 35.5 86-87 24.36070950137187 31.0 14.5 34.0 2.0 35.0 88-89 23.578308573691626 30.5 7.0 34.0 2.0 35.0 90-91 22.76688013513169 30.0 2.0 34.0 2.0 35.0 92-93 21.960256912222906 29.0 2.0 34.0 2.0 35.0 94-95 21.024013477165774 29.0 2.0 34.0 2.0 35.0 96-97 19.823002839745637 27.5 2.0 33.5 2.0 35.0 98-99 18.367488737775815 25.0 2.0 33.0 2.0 35.0 100-101 14.91710252936879 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.558492030369198 1101 2 0.5619479807809853 1101 3 0.5681607094706749 1101 4 0.4579866092567997 1101 5 0.4796516046208481 1101 6 0.561377840121196 1101 7 0.5232474645124157 1101 8 0.5204928443884711 1101 9 0.5723607308334522 1101 10-11 0.6208181725938218 1101 12-13 0.6621413656354207 1101 14-15 0.723832795639197 1101 16-17 0.7087113509368663 1101 18-19 0.6864086159626197 1101 20-21 0.6713268376326198 1101 22-23 0.8582156429690571 1101 24-25 0.8766189443096977 1101 26-27 0.7912358847494616 1101 28-29 0.8456275660931496 1101 30-31 0.7859305692261742 1101 32-33 0.8075181743996396 1101 34-35 0.6709032193477285 1101 36-37 0.7685177677552488 1101 38-39 0.7508562115763979 1101 40-41 0.6332674492406198 1101 42-43 0.7528687407977515 1101 44-45 0.807618289692769 1101 46-47 0.8110905604401708 1101 48-49 0.8712116570190673 1101 50-51 0.7631885580800386 1101 52-53 0.8758469404384073 1101 54-55 0.8380269832985903 1101 56-57 0.8528607754467643 1101 58-59 0.9565255513889142 1101 60-61 0.8875091634134193 1101 62-63 0.787761792378948 1101 64-65 0.6287079449835851 1101 66-67 0.7002930763773882 1101 68-69 0.7637187199834727 1101 70-71 1.0038532913922396 1101 72-73 1.0170556651245697 1101 74-75 0.985163857264034 1101 76-77 1.1489841085143695 1101 78-79 0.805671493369271 1101 80-81 1.102596233962668 1101 82-83 1.165017551644599 1101 84-85 1.0368415723652227 1101 86-87 1.198209600769843 1101 88-89 1.1711185441076353 1101 90-91 1.027883847185862 1101 92-93 1.0874319948167113 1101 94-95 0.9514369898932813 1101 96-97 1.2066050394452894 1101 98-99 1.4281923995838604 1101 100-101 1.0939037785739583 1102 1 0.5016815330389761 1102 2 0.5736279106689466 1102 3 0.5855791690615817 1102 4 0.4989948485033082 1102 5 0.5465866914764632 1102 6 0.5580886333718311 1102 7 0.35346515806438106 1102 8 0.5045580442564699 1102 9 0.5926045000047111 1102 10-11 0.5902976610356134 1102 12-13 0.653649207255782 1102 14-15 0.5593007772672536 1102 16-17 0.6169868436416408 1102 18-19 0.7507834773344584 1102 20-21 0.7313731191519253 1102 22-23 0.7822406474322037 1102 24-25 0.5814552413553642 1102 26-27 0.5518744128294415 1102 28-29 0.45084701785595627 1102 30-31 0.5480730438132682 1102 32-33 0.5718435353738514 1102 34-35 0.5576920230610654 1102 36-37 0.5418397583745147 1102 38-39 0.5423777131425567 1102 40-41 0.5863191622789685 1102 42-43 0.6074931058131412 1102 44-45 0.5486710251983453 1102 46-47 0.5988231176361225 1102 48-49 0.25960787118537354 1102 50-51 0.4770539440478885 1102 52-53 0.4664502502026977 1102 54-55 0.554854278304731 1102 56-57 0.6656106442571854 1102 58-59 0.7333828982757993 1102 60-61 0.6668273292478659 1102 62-63 0.6803046791988585 1102 64-65 0.6792267524297451 1102 66-67 0.6860738842887955 1102 68-69 0.5227139971586503 1102 70-71 0.5247969637266543 1102 72-73 0.623269858484484 1102 74-75 0.6630576018203449 1102 76-77 0.677563149559127 1102 78-79 0.5449619606205651 1102 80-81 0.6851496429982511 1102 82-83 0.7491540266752992 1102 84-85 0.5323655732957207 1102 86-87 0.5061805172575333 1102 88-89 0.6751117114297998 1102 90-91 0.7573641741589832 1102 92-93 0.7540150337624318 1102 94-95 0.6103691067250381 1102 96-97 0.753409288364665 1102 98-99 0.9987230276167729 1102 100-101 0.7496149883853178 1103 1 0.479164639851426 1103 2 0.49083592636581486 1103 3 0.45483839258115566 1103 4 0.45321224421834216 1103 5 0.5052310796721358 1103 6 0.440619827932359 1103 7 0.38276951256028013 1103 8 0.37495187635636995 1103 9 0.4489810071541598 1103 10-11 0.49423756557766296 1103 12-13 0.4402850443191042 1103 14-15 0.7082493789245206 1103 16-17 0.6799216437317099 1103 18-19 0.5480246979492236 1103 20-21 0.4856887935062062 1103 22-23 0.6133824561847447 1103 24-25 0.7290486765797937 1103 26-27 0.7340725684966714 1103 28-29 0.6064478363497585 1103 30-31 0.7090571168343942 1103 32-33 0.8974937158730114 1103 34-35 0.9661569512944084 1103 36-37 0.8601809475658442 1103 38-39 0.8051621756162959 1103 40-41 0.8786963325370678 1103 42-43 0.7188817988210587 1103 44-45 0.8778687874541049 1103 46-47 0.752369882878348 1103 48-49 0.7885937355895507 1103 50-51 0.9555907997747468 1103 52-53 1.012407687535223 1103 54-55 0.9740042126124351 1103 56-57 1.0909941956988263 1103 58-59 0.9506978526128549 1103 60-61 0.9960114829307116 1103 62-63 1.1432961404379363 1103 64-65 0.9984431460589569 1103 66-67 1.2294897350596585 1103 68-69 1.1643095652175965 1103 70-71 1.1785261333315376 1103 72-73 1.2678499102960323 1103 74-75 1.1148599954446041 1103 76-77 1.1825344354702914 1103 78-79 1.2582794090378862 1103 80-81 1.1970909430375904 1103 82-83 1.29324001306178 1103 84-85 1.1925235811985893 1103 86-87 1.3025866663530579 1103 88-89 1.2511980343191702 1103 90-91 1.3360031684112137 1103 92-93 1.3794297612074615 1103 94-95 1.3888046156980387 1103 96-97 1.2449789011636518 1103 98-99 1.1916684918794225 1103 100-101 1.0332449143794218 1104 1 0.44095028783965873 1104 2 0.4108160941874033 1104 3 0.4216227834348558 1104 4 0.40280721192056035 1104 5 0.43162521837049894 1104 6 0.48397221801533163 1104 7 0.45483344548804183 1104 8 0.36303076331060424 1104 9 0.36359295204702136 1104 10-11 0.4485426756532931 1104 12-13 0.4168597169354271 1104 14-15 0.5055843477076394 1104 16-17 0.5319648515630746 1104 18-19 0.5887056654023581 1104 20-21 0.6244332568272455 1104 22-23 0.6922686114913787 1104 24-25 0.6828830308943523 1104 26-27 0.6153415459410922 1104 28-29 0.6144700465097586 1104 30-31 0.6357966738060838 1104 32-33 0.7057010420301069 1104 34-35 0.5905336592937758 1104 36-37 0.6197123536062037 1104 38-39 0.6607161489838447 1104 40-41 0.5912288124287883 1104 42-43 0.5435736598736227 1104 44-45 0.5488984393805794 1104 46-47 0.48497854277293584 1104 48-49 0.6156366496978976 1104 50-51 0.40272873915501606 1104 52-53 0.44121966015553227 1104 54-55 0.6356854831877357 1104 56-57 0.6555551737729957 1104 58-59 0.578301519216069 1104 60-61 0.6641873219798953 1104 62-63 0.7379719524646013 1104 64-65 0.6650178891687979 1104 66-67 0.5715255880665637 1104 68-69 0.6421216811747463 1104 70-71 0.7171902810295698 1104 72-73 0.65927197617928 1104 74-75 0.4860652702401147 1104 76-77 0.5885453243701484 1104 78-79 0.8533969804889487 1104 80-81 0.710924169455744 1104 82-83 0.6458369240355033 1104 84-85 0.7646151005925361 1104 86-87 0.7907464328785068 1104 88-89 0.786258083053692 1104 90-91 0.7815163730233721 1104 92-93 0.821712670522242 1104 94-95 0.677008041213405 1104 96-97 0.7392574116824626 1104 98-99 0.653494415168371 1104 100-101 0.5365473950465738 1105 1 0.4735645070520178 1105 2 0.5393033438948827 1105 3 0.54394906860745 1105 4 0.5620788331154927 1105 5 0.3928420319848698 1105 6 0.4417757285238153 1105 7 0.38177833780854087 1105 8 0.44238741520587865 1105 9 0.5830541059510068 1105 10-11 0.5731719867746321 1105 12-13 0.5689327870702812 1105 14-15 0.6438058289053146 1105 16-17 0.5603409624070821 1105 18-19 0.5296948324086159 1105 20-21 0.5002215055930037 1105 22-23 0.5464814804274525 1105 24-25 0.6964351433196825 1105 26-27 0.7525981312455272 1105 28-29 0.7199693173360231 1105 30-31 0.6369615467406433 1105 32-33 0.6110531265529033 1105 34-35 0.7027193567828363 1105 36-37 0.6996429280507002 1105 38-39 0.6768569370890063 1105 40-41 0.7354095472150348 1105 42-43 0.8149397484800787 1105 44-45 0.6976849148505835 1105 46-47 0.8116611797418685 1105 48-49 0.7518162132535551 1105 50-51 0.6072516792069891 1105 52-53 0.5737069841986298 1105 54-55 0.6219635814514177 1105 56-57 0.719023920935939 1105 58-59 0.7515765571819202 1105 60-61 0.7892552221436091 1105 62-63 0.7810504660829984 1105 64-65 0.8990620731579888 1105 66-67 1.0511511375032612 1105 68-69 0.9528803570795397 1105 70-71 1.0823885495075949 1105 72-73 1.1348016863744022 1105 74-75 0.9571873050514128 1105 76-77 0.9176913957282515 1105 78-79 1.0452102191824864 1105 80-81 1.0892364948259505 1105 82-83 1.2092689924392168 1105 84-85 1.02621383567773 1105 86-87 1.1721322604371167 1105 88-89 1.198178644241377 1105 90-91 1.0396558342910929 1105 92-93 1.1893003045253074 1105 94-95 1.2443859883072506 1105 96-97 1.1576146282972566 1105 98-99 1.3314444610990677 1105 100-101 1.0856723465528102 1106 1 0.34914675076376867 1106 2 0.41603568784464784 1106 3 0.20934047964138003 1106 4 0.4537846950233515 1106 5 0.4722913062672447 1106 6 0.0513771326431538 1106 7 0.3060491786306443 1106 8 0.4227395925161801 1106 9 0.5174604347033522 1106 10-11 0.19904376712804606 1106 12-13 0.25726178850514714 1106 14-15 0.5805988124367474 1106 16-17 0.2295996401538929 1106 18-19 0.07258045227241183 1106 20-21 0.32286231765647244 1106 22-23 0.4159584848072839 1106 24-25 0.5226563092571936 1106 26-27 0.2857958839409136 1106 28-29 0.3297573175662194 1106 30-31 0.41806725398406996 1106 32-33 0.5544406449129653 1106 34-35 0.24235850168200557 1106 36-37 0.13223222961713077 1106 38-39 0.268054244787443 1106 40-41 0.43499925442137766 1106 42-43 0.0596868077656012 1106 44-45 0.33023611005398834 1106 46-47 0.11835080118485308 1106 48-49 0.35016797530332866 1106 50-51 0.14046259464654298 1106 52-53 0.38034447942549576 1106 54-55 0.040384653876600396 1106 56-57 -0.21931588182054895 1106 58-59 -0.5771554568683683 1106 60-61 0.12801506074824687 1106 62-63 0.11518642070030438 1106 64-65 0.21538411206968888 1106 66-67 -0.1634858165205415 1106 68-69 -0.33240480568282393 1106 70-71 -0.2695478082307794 1106 72-73 0.30426016670628897 1106 74-75 0.15370913080536752 1106 76-77 0.003953636424380846 1106 78-79 0.16174392901403323 1106 80-81 -0.016987768948098392 1106 82-83 0.1089764471703063 1106 84-85 0.24751463665476336 1106 86-87 0.3064208941407749 1106 88-89 0.1003214690239993 1106 90-91 0.2465577301285684 1106 92-93 0.2132759150536181 1106 94-95 0.048756911464288066 1106 96-97 0.0551756811278743 1106 98-99 -0.12682286172821122 1106 100-101 -0.3772917582502746 1107 1 0.3772966118688217 1107 2 0.40195712129869676 1107 3 0.36438651131815547 1107 4 0.4149668292060511 1107 5 0.3502528229128288 1107 6 0.3731552788682606 1107 7 0.3886080453642151 1107 8 0.33316325847019357 1107 9 0.4150199559157386 1107 10-11 0.44502599439218216 1107 12-13 0.36791597644237584 1107 14-15 0.47559859427714457 1107 16-17 0.4386983501034223 1107 18-19 0.42092316646878913 1107 20-21 0.49572471712124866 1107 22-23 0.512170506855945 1107 24-25 0.39281498401031456 1107 26-27 0.36858381826032627 1107 28-29 0.3467919467676097 1107 30-31 0.4811917358549991 1107 32-33 0.5513318705336232 1107 34-35 0.4785090027218928 1107 36-37 0.5767360619542004 1107 38-39 0.6430124308630454 1107 40-41 0.5776356405348011 1107 42-43 0.5049869226629866 1107 44-45 0.6069461177622912 1107 46-47 0.5790384402615985 1107 48-49 0.40592593449293446 1107 50-51 0.4957136963525528 1107 52-53 0.21919725077969332 1107 54-55 0.38854193240823776 1107 56-57 0.3082413123560457 1107 58-59 0.3473758635790034 1107 60-61 0.4146331505427412 1107 62-63 0.4188245957224872 1107 64-65 0.42313023246470394 1107 66-67 0.43091911679533723 1107 68-69 0.33930861985930605 1107 70-71 0.366427437925978 1107 72-73 0.3939928484695976 1107 74-75 0.489656743412084 1107 76-77 0.6476771845722062 1107 78-79 0.5280884466631441 1107 80-81 0.48867347281637663 1107 82-83 0.5747544770436299 1107 84-85 0.6239454000679423 1107 86-87 0.4379638643110866 1107 88-89 0.39793843893252046 1107 90-91 0.49718675699128667 1107 92-93 0.7061493679384121 1107 94-95 0.6049948217861463 1107 96-97 0.7042623195792324 1107 98-99 0.9153837499622348 1107 100-101 0.6967843358201495 1108 1 0.4173425375681781 1108 2 0.41162752358252774 1108 3 0.43160374432864756 1108 4 0.3947832683737005 1108 5 0.5086006592684029 1108 6 0.4411989744254683 1108 7 0.3424750447453775 1108 8 0.41981099215921347 1108 9 0.2547788217955329 1108 10-11 0.40750277181096095 1108 12-13 0.5036900764645083 1108 14-15 0.63873605776147 1108 16-17 0.574738957275116 1108 18-19 0.652635645908056 1108 20-21 0.5332875447298449 1108 22-23 0.5373974573753131 1108 24-25 0.49032748177597796 1108 26-27 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-0.917410406735625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10208.0 3 3385.0 4 3628.0 5 4030.0 6 4832.0 7 6266.0 8 8052.0 9 10298.0 10 13043.0 11 16667.0 12 20531.0 13 24329.0 14 27870.0 15 33013.0 16 38036.0 17 43767.0 18 41420.0 19 38566.0 20 39616.0 21 42576.0 22 46923.0 23 51935.0 24 58129.0 25 65474.0 26 73653.0 27 84592.0 28 97308.0 29 112062.0 30 129692.0 31 152339.0 32 180251.0 33 218542.0 34 270235.0 35 337489.0 36 405657.0 37 407455.0 38 199144.0 39 12032.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71996590374903 18.242275744412773 14.542243403469499 23.495514948368708 2 15.531669515341118 19.759024354515585 39.9274293429678 24.781876787175502 3 19.189721297288795 23.493285698719873 29.757485507152847 27.55950749683848 4 12.931755190308042 16.18377087087547 36.34670035965403 34.53777357916246 5 13.75654831417498 37.36959660883636 33.93232082761685 14.941534249371813 6 32.89977579579284 35.61697587312948 17.033905632485233 14.44934269859245 7 29.145718387294377 32.790936597058 19.932517302120733 18.130827713526884 8 27.236403602496107 34.14258839969363 19.509939038005804 19.111068959804463 9 26.847777312544927 16.496946721834277 19.615147381264322 37.04012858435647 10-11 25.221644876245808 26.238836339449417 27.046880583331046 21.49263820097373 12-13 26.455996840087664 23.54088166596533 27.23991202189368 22.763209472053326 14-15 22.655465443419935 25.075167367068257 25.70378805113482 26.565579138376982 16-17 22.2694566757319 28.32161425683168 26.741028292502268 22.667900774934157 18-19 22.541729547629252 26.339109377457458 29.19468546312204 21.924475611791248 20-21 22.771999040020603 25.610587269946777 29.55049520137879 22.066918488653826 22-23 22.479543728221664 25.70205846698818 29.52056253227775 22.297835272512405 24-25 22.34043543319947 25.880852131890936 29.57743476548239 22.201277669427206 26-27 21.686743913749112 26.311587647731333 29.204533361791047 22.79713507672851 28-29 22.0504746159129 26.957445454589113 28.90717674970344 22.084903179794544 30-31 22.054616462988143 26.273298451322784 28.84053337656061 22.831551709128462 32-33 21.548112696113247 26.283992812557972 28.961783244470883 23.206111246857898 34-35 22.731601420378947 26.48768995407137 28.591077319818954 22.18963130573073 36-37 22.848713276484478 25.744045675994542 28.661245328820005 22.745995718700975 38-39 22.127129091775576 26.21764422724877 28.61307879665275 23.042147884322898 40-41 22.745427503581713 25.99173040322237 28.82534873294148 22.437493360254436 42-43 22.357615556187415 26.177176025921977 28.512479715632487 22.95272870225812 44-45 22.27958621713924 26.43420196930158 28.86800788800769 22.418203925551488 46-47 22.919972016393483 26.856655730975703 27.792888305171942 22.430483947458875 48-49 22.469111983014294 26.897089553044935 27.675996284076138 22.957802179864636 50-51 22.77401678852792 27.076602159416602 27.69684032895337 22.45254072310211 52-53 23.160996528799323 26.53562410273806 27.87741562155826 22.42596374690436 54-55 22.33029533059359 27.010398863134444 28.110627148426246 22.548678657845716 56-57 22.46311147810933 27.12308536149191 27.720100913841566 22.69370224655719 58-59 22.446165779244062 26.488642040876954 27.788014768872543 23.27717741100644 60-61 21.79365059122971 26.90027297911811 28.256736898090047 23.049339531562133 62-63 22.199259295597223 26.813517382790437 28.420672214673758 22.56655110693858 64-65 21.844759092900308 27.046615178737525 28.013664104272056 23.09496162409011 66-67 21.67567106157548 27.02383608075767 28.12601122232692 23.174481635339934 68-69 22.6766762438671 26.412414524198773 28.238118677971276 22.67279055396286 70-71 22.28552273791936 26.764894502308202 27.59917989420371 23.350402865568732 72-73 22.227001939761756 26.921666514622633 27.70888449294916 23.14244705266645 74-75 22.289021857975154 27.346363463540108 27.599457860638132 22.765156817846606 76-77 22.515259756432023 26.788951762306073 27.7739419592141 22.921846522047808 78-79 22.532256929687268 27.194744138605298 27.61345001490449 22.659548916802947 80-81 22.862796994087457 27.20884629180923 27.214982215572554 22.713374498530754 82-83 22.643751826508268 27.309486561399783 27.373920672383072 22.672840939708873 84-85 22.53252484228709 27.49154596383813 27.377665322332025 22.598263871542756 86-87 22.956908257611595 27.51884585800694 26.80737880583414 22.716867078547327 88-89 22.669042398072165 27.58999665818984 26.6459165315762 23.095044412161787 90-91 22.56036700799791 27.569629998131646 26.86891424549588 23.001088748374567 92-93 22.906335273847116 27.036706710332474 27.171194248740875 22.885763767079528 94-95 22.57526084860224 26.764189802313144 27.465398384705225 23.19515096437939 96-97 23.00681874047638 26.283589852715327 27.422762809936952 23.286828596871345 98-99 22.727229077639603 26.688808397752915 26.852013011955634 23.731949512651845 100-101 23.165354988268042 26.128114015838673 27.350649951441014 23.355881044452275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 622.0 1 430.0 2 383.0 3 514.5 4 463.5 5 474.5 6 832.5 7 1840.5 8 2968.5 9 3280.5 10 2835.0 11 2388.5 12 2342.5 13 2487.0 14 2725.0 15 3048.0 16 3295.0 17 3583.0 18 4074.5 19 4672.0 20 5390.0 21 6439.0 22 7932.0 23 9693.5 24 11794.5 25 14856.5 26 18802.5 27 23471.5 28 27995.0 29 32501.0 30 37864.0 31 46740.0 32 60764.5 33 65019.0 34 61902.0 35 64884.5 36 70870.5 37 77448.5 38 83287.0 39 87550.0 40 92528.5 41 99328.5 42 103130.0 43 105583.0 44 109056.5 45 116457.0 46 121239.0 47 123218.0 48 124297.5 49 121758.5 50 120208.0 51 117456.0 52 114155.5 53 115310.0 54 119063.5 55 114446.0 56 104931.0 57 96270.5 58 89716.5 59 78896.0 60 61724.5 61 51022.5 62 44790.0 63 34080.0 64 23376.0 65 17440.5 66 13172.0 67 10152.0 68 7944.0 69 6215.0 70 4847.0 71 3458.0 72 2475.5 73 1953.0 74 1523.0 75 1139.0 76 864.0 77 607.0 78 393.5 79 254.5 80 146.5 81 77.0 82 54.0 83 43.0 84 28.5 85 13.5 86 7.0 87 7.5 88 5.5 89 3.0 90 3.5 91 4.5 92 4.5 93 2.0 94 1.0 95 1.5 96 2.5 97 2.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1798055531803501 2 0.05013433662011764 3 0.047224084883342406 4 0.0536446402613826 5 0.061055281281830874 6 0.06459558751832034 7 0.03684318693566994 8 0.03399294038934368 9 0.04140358140979195 10-11 0.04261868651638367 12-13 0.039213391958404405 14-15 0.03441297672248649 16-17 0.03253781452095606 18-19 0.0405935113387308 20-21 0.0517094728694032 22-23 0.0482891770138117 24-25 0.0687959508497485 26-27 0.053599636368545873 28-29 0.045273916193750756 30-31 0.054454710332443756 32-33 0.05037435738191354 34-35 0.05041936127475027 36-37 0.04738909915707709 38-39 0.02619226563097708 40-41 0.025172177393344528 42-43 0.022126913978059104 44-45 0.004125356843366951 46-47 0.011385984887692786 48-49 0.010830936876039777 50-51 0.008790760400774667 52-53 0.010095873293039848 54-55 0.01119096801873362 56-57 0.009030781162570563 58-59 0.010800934280815291 60-61 0.011175966721121377 62-63 0.004095354248142464 64-65 0.006750583925509556 66-67 0.0040653516529179775 68-69 0.00910578765063178 70-71 0.023762055417793637 72-73 0.020311756966977644 74-75 0.020746794597732703 76-77 0.012691097779957966 78-79 0.004695406152632203 80-81 0.006405554080427957 82-83 0.005490474926081105 84-85 0.005085439890550532 86-87 0.008505735746142041 88-89 0.030557643236139923 90-91 0.037128211590302565 92-93 0.02298198794195698 94-95 0.03250781192573157 96-97 0.035943109078935324 98-99 0.021751881537753016 100-101 0.1231306508012943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3333045.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.42174697578295 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88328174569354 37.757458848976256 2 13.486897337841638 13.600658501140236 3 4.849879938187414 7.336182573186348 4 2.227190337380808 4.491953106332951 5 1.268970232384428 3.199184798854407 6 0.7421213467087467 2.2451432861445437 7 0.5076591310658429 1.7917942179583435 8 0.3522049054021534 1.420702929905356 9 0.24536499890937427 1.1134558702549555 >10 1.2924281849442762 11.51121546450056 >50 0.0876088577031734 3.056084300652036 >100 0.047005167363736076 4.656633434186876 >500 0.004962985749077402 1.7028543868150336 >1k 0.003946470593923821 4.4017037202248135 >5k 4.185650629919204E-4 1.3761523655326648 >10k+ 5.979500899884578E-5 0.338822195334679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11238 0.337169165132784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8440 0.25322190369466957 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6782 0.20347760081247027 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6592 0.19777710771981777 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6555 0.19666701169651174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6155 0.18466597360671697 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5767 0.17302496665961606 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5353 0.16060389223667848 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4987 0.14962294238451626 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4958 0.14875286712300614 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4810 0.14431248302978208 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4602 0.1380719432230888 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4250 0.1275110297040694 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3917 0.11752016549431525 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3611 0.10833937135562226 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3581 0.10743929349888764 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3405 0.10215883673937796 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3390 0.10170879781101064 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3372 0.10116875109696989 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3343 0.10029867583545976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.000259522448692E-5 2 0.0 0.0 0.0 0.0 3.000259522448692E-5 3 0.0 0.0 0.0 0.0 3.000259522448692E-5 4 0.0 0.0 0.0 0.0 3.000259522448692E-5 5 0.0 0.0 0.0 0.0 6.000519044897384E-5 6 0.0 0.0 0.0 0.0 9.000778567346076E-5 7 6.000519044897384E-5 0.0 0.0 0.0 9.000778567346076E-5 8 9.000778567346076E-5 0.0 0.0 0.0 9.000778567346076E-5 9 9.000778567346076E-5 0.0 0.0 3.000259522448692E-5 9.000778567346076E-5 10-11 9.000778567346076E-5 0.0 0.0 3.000259522448692E-5 9.000778567346076E-5 12-13 9.000778567346076E-5 0.0 0.0 3.000259522448692E-5 4.950428212040341E-4 14-15 1.2001038089794767E-4 0.0 0.0 4.500389283673038E-5 5.400467140407645E-4 16-17 1.2001038089794767E-4 0.0 0.0 9.000778567346076E-5 5.55048011653008E-4 18-19 1.2001038089794767E-4 0.0 0.0 1.0500908328570422E-4 5.700493092652514E-4 20-21 1.2001038089794767E-4 0.0 0.0 1.3501167851019113E-4 6.300544997142253E-4 22-23 1.2001038089794767E-4 0.0 0.0 3.750324403060865E-4 6.300544997142253E-4 24-25 1.2001038089794767E-4 0.0 0.0 8.700752615101206E-4 6.300544997142253E-4 26-27 1.2001038089794767E-4 0.0 0.0 0.0014101219755508852 6.450557973264688E-4 28-29 1.6501427373467805E-4 0.0 0.0 0.004020347760081247 6.600570949387122E-4 30-31 1.8001557134692152E-4 0.0 0.0 0.012871113351304888 6.600570949387122E-4 32-33 1.8001557134692152E-4 0.0 0.0 0.032102776890201 6.600570949387122E-4 34-35 1.8001557134692152E-4 0.0 0.0 0.06104027998421863 6.900596901631991E-4 36-37 2.1001816657140843E-4 0.0 0.0 0.10782932723680598 6.900596901631991E-4 38-39 2.1001816657140843E-4 0.0 0.0 0.18279081140518655 6.900596901631991E-4 40-41 2.1001816657140843E-4 0.0 0.0 0.2599874889177914 6.900596901631991E-4 42-43 2.4002076179589535E-4 0.0 0.0 0.3446548126412935 7.050609877754426E-4 44-45 2.4002076179589535E-4 0.0 0.0 0.44925886089146716 7.50064880612173E-4 46-47 2.4002076179589535E-4 0.0 0.0 0.576184839988659 7.800674758366599E-4 48-49 2.4002076179589535E-4 0.0 0.0 0.7087063030952176 7.800674758366599E-4 50-51 2.4002076179589535E-4 0.0 0.0 0.8566340988495504 8.700752615101206E-4 52-53 2.4002076179589535E-4 0.0 0.0 0.9983513573924145 9.000778567346075E-4 54-55 2.4002076179589535E-4 0.0 0.0 1.1495794386214406 9.600830471835814E-4 56-57 2.4002076179589535E-4 0.0 0.0 1.3274048205169748 9.900856424080683E-4 58-59 2.4002076179589535E-4 0.0 0.0 1.51654118081214 0.001050090832857042 60-61 2.4002076179589535E-4 0.0 0.0 1.7316297859764869 0.001080093428081529 62-63 2.4002076179589535E-4 0.0 0.0 1.9378376229543854 0.0012151051065917202 64-65 2.4002076179589535E-4 0.0 0.0 2.1512760853813857 0.0012301064042039636 66-67 3.000259522448692E-4 0.0 0.0 2.4056230863969734 0.0012301064042039636 68-69 3.000259522448692E-4 0.0 0.0 2.6871674399835586 0.0012301064042039636 70-71 3.1502724985711265E-4 0.0 0.0 2.9797677499103674 0.001275110297040694 72-73 3.450298450815996E-4 0.0 0.0 3.2959200970883984 0.0013801193803263984 74-75 3.6003114269384304E-4 1.500129761224346E-5 0.0 3.609927258707878 0.0014551258683876155 76-77 3.6003114269384304E-4 3.000259522448692E-5 0.0 3.9496466444347433 0.0015151310588365893 78-79 3.6003114269384304E-4 3.000259522448692E-5 0.0 4.329854532417054 0.0015901375468978067 80-81 4.2003633314281687E-4 6.000519044897384E-5 0.0 4.727343915248669 0.00160513884451005 82-83 4.2003633314281687E-4 6.000519044897384E-5 0.0 5.142069789036752 0.0016501427373467805 84-85 4.2003633314281687E-4 6.000519044897384E-5 0.0 5.572502021424853 0.0016501427373467805 86-87 4.2003633314281687E-4 6.000519044897384E-5 0.0 6.0150853048788715 0.0018301583086937018 88-89 4.2003633314281687E-4 6.000519044897384E-5 0.0 6.476270197372073 0.0020101738800406234 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6135 0.0 53.0697 1 GTATCAA 11445 0.0 42.463425 1 TATCAAC 14360 0.0 33.559563 2 ATCAACG 14635 0.0 32.895992 3 TCAACGC 14730 0.0 32.65157 4 CAACGCA 14980 0.0 31.946579 5 AACGCAG 15535 0.0 30.828913 6 GTGTAAG 1395 0.0 29.003458 1 ACGCAGA 17325 0.0 27.338753 7 TAAGGTG 2750 0.0 27.304304 5 CGCAGAG 17370 0.0 27.185873 8 AGGTAAG 2460 0.0 26.467825 2 TAAGGTA 1460 0.0 25.715828 4 GTACATG 12870 0.0 24.964928 1 TACATGG 12720 0.0 24.734531 2 GCAGAGT 19090 0.0 24.038197 9 AAGGTAA 2635 0.0 23.845058 1 ACATGGG 13135 0.0 23.120493 3 GGTAAGG 2990 0.0 22.888512 3 GTAAGGT 3040 0.0 22.668392 4 >>END_MODULE