FastQCFastQC Report
Thu 26 May 2016
SRR938358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333045
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108940.3268482723755605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91840.27554383454168785No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA69340.20803799528659228No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA69330.2080079926913678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66730.20020731793300123No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62840.1885363083906758No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC61540.18463597101149248No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56720.1701747201132898No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53710.16114393895071924No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46860.1405921612219457No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA44750.13426161362957895No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG43230.12970121915545696No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42860.12859112313215093No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40380.12115047951647819No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39240.11773018366088668No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35720.10716927014186726No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35070.10521910145227563No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34650.10395899245284716No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34440.10332893795313294No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33710.1011387485017454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.055.2471161
GTATCAA111000.042.553361
TATCAAC146050.032.210862
ATCAACG145450.032.162343
TCAACGC147600.031.629434
CAACGCA151700.030.8059225
AGGTAAG21800.029.893152
AACGCAG157700.029.6359626
TAAGGTG25400.028.075265
ACGCAGA179800.025.8611987
AAGGTAA24050.025.7120021
TGGTATC27250.025.6601072
GTAAGGT26550.025.6057664
CGCAGAG181350.025.587788
GGTAAGG26700.024.7496933
GTGGTAT33050.023.8915441
AGGACCG11600.023.3605525
GTACATG131100.022.1690031
GCAGAGT205250.022.0760239
GGACCGA10200.021.885346
Produced by FastQC (version 0.11.5)