##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938358_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3333045 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812404572995565 31.0 31.0 34.0 30.0 34.0 2 32.03905827854109 31.0 31.0 34.0 30.0 34.0 3 32.10084682325021 33.0 31.0 34.0 30.0 34.0 4 35.77052725060718 37.0 35.0 37.0 35.0 37.0 5 35.637129711720064 37.0 35.0 37.0 33.0 37.0 6 35.66794357711942 37.0 35.0 37.0 33.0 37.0 7 35.60854383904208 37.0 35.0 37.0 33.0 37.0 8 35.59258545864217 37.0 35.0 37.0 33.0 37.0 9 37.17957003280784 39.0 37.0 39.0 33.0 39.0 10-11 37.09152141660254 39.0 37.0 39.0 33.0 39.0 12-13 36.98924421962499 39.0 37.0 39.0 33.0 39.0 14-15 38.04980550817646 40.0 37.0 41.0 33.0 41.0 16-17 37.97581040760026 40.0 37.0 41.0 33.0 41.0 18-19 38.09269151781629 40.0 37.5 41.0 33.0 41.0 20-21 38.14066866783977 40.0 37.0 41.0 33.0 41.0 22-23 38.04911079808404 40.0 37.0 41.0 33.0 41.0 24-25 37.941536642919615 40.0 37.0 41.0 33.0 41.0 26-27 37.74873951596813 40.0 37.0 41.0 32.0 41.0 28-29 37.63010550412611 40.0 37.0 41.0 32.0 41.0 30-31 37.38420423366621 39.0 36.0 41.0 31.5 41.0 32-33 37.153630989080554 39.0 36.0 41.0 31.0 41.0 34-35 36.987412411173565 39.0 36.0 41.0 31.0 41.0 36-37 36.962450851998696 39.0 36.0 41.0 31.0 41.0 38-39 36.82610900842923 39.0 35.0 41.0 30.0 41.0 40-41 36.64044574855725 39.0 35.0 41.0 30.0 41.0 42-43 36.41384574765717 39.0 35.0 40.5 30.0 41.0 44-45 36.141825417898644 39.0 35.0 40.0 29.5 41.0 46-47 35.76401713748239 38.0 35.0 40.0 28.5 41.0 48-49 35.38181182672301 38.0 34.5 40.0 27.0 41.0 50-51 35.00394699141475 38.0 34.0 40.0 26.0 41.0 52-53 34.50944241676905 38.0 33.0 40.0 24.5 41.0 54-55 34.18956854767937 38.0 33.0 40.0 24.0 41.0 56-57 33.84267434133052 37.0 33.0 40.0 23.0 41.0 58-59 33.39111668159296 36.5 32.0 39.5 22.5 41.0 60-61 33.02034400975684 36.0 32.0 39.0 22.0 40.5 62-63 32.56664596487596 35.5 31.0 39.0 20.5 40.0 64-65 32.06904272219547 35.0 31.0 38.5 18.5 40.0 66-67 31.584518210825237 35.0 30.0 38.0 17.5 40.0 68-69 31.050563523744806 34.5 29.5 37.5 13.5 40.0 70-71 30.467764461625933 34.0 29.0 37.0 9.5 39.0 72-73 30.032660525135423 34.0 29.0 36.0 8.0 39.0 74-75 29.538224206393853 34.0 28.0 36.0 7.0 38.5 76-77 28.2696217722833 31.5 26.5 34.5 6.0 36.5 78-79 28.96962267236116 33.5 28.0 35.0 2.0 37.0 80-81 28.713199641769016 33.0 28.0 35.0 2.0 37.0 82-83 28.25369114428398 33.0 27.0 35.0 2.0 36.0 84-85 27.749661195693427 33.0 26.0 35.0 2.0 36.0 86-87 27.200625854136383 32.5 25.5 35.0 2.0 35.0 88-89 26.561634931421565 32.0 25.0 34.0 2.0 35.0 90-91 26.027638240707823 32.0 24.0 34.0 2.0 35.0 92-93 25.32455562406148 31.0 21.0 34.0 2.0 35.0 94-95 24.588191128532618 31.0 18.5 34.0 2.0 35.0 96-97 23.61553564383319 31.0 4.5 34.0 2.0 35.0 98-99 22.387962658769986 30.5 2.0 34.0 2.0 35.0 100-101 18.69265701483178 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3705465351026511 1101 2 0.3823092821726348 1101 3 0.37754254352883265 1101 4 0.2023560838491818 1101 5 0.17875900646921394 1101 6 0.20586081404309198 1101 7 0.26420006835938636 1101 8 0.3108626071747409 1101 9 0.2848332661416535 1101 10-11 0.3060275695999479 1101 12-13 0.39264688690275307 1101 14-15 0.4112620860874898 1101 16-17 0.4089503490696771 1101 18-19 0.3999465551269523 1101 20-21 0.36381019373140333 1101 22-23 0.38417186393214564 1101 24-25 0.4009642850971673 1101 26-27 0.47824882820737 1101 28-29 0.3982598706144813 1101 30-31 0.4296378296491099 1101 32-33 0.40763464906448377 1101 34-35 0.4190653005767402 1101 36-37 0.5661628463085009 1101 38-39 0.6759892581260303 1101 40-41 0.6312357941012294 1101 42-43 0.747240809246918 1101 44-45 0.7009079683270016 1101 46-47 0.6794165644817198 1101 48-49 0.5886185824678805 1101 50-51 0.7698909085035837 1101 52-53 0.9316607313289254 1101 54-55 0.7787467731326814 1101 56-57 0.8247058024941154 1101 58-59 0.840720640712739 1101 60-61 0.6659676738238076 1101 62-63 0.6733237153479337 1101 64-65 0.5544079377019884 1101 66-67 0.49091046477741074 1101 68-69 0.5372007623413353 1101 70-71 0.3595851378636503 1101 72-73 0.61009274371537 1101 74-75 0.7079637982854372 1101 76-77 0.7558348343770902 1101 78-79 0.7397562400203697 1101 80-81 0.779651531794034 1101 82-83 0.7968465929852826 1101 84-85 1.0901136544561645 1101 86-87 1.0019215439445368 1101 88-89 1.0902115758505708 1101 90-91 1.1464970023394336 1101 92-93 1.0252571251751093 1101 94-95 1.217468105717309 1101 96-97 1.0723296625426109 1101 98-99 0.9727809179810407 1101 100-101 0.8595097120246074 1102 1 0.3201453925360944 1102 2 0.35474729847801 1102 3 0.3200368766800352 1102 4 0.2548697843690775 1102 5 0.18300170448432596 1102 6 0.169089862932168 1102 7 0.24607697196073985 1102 8 0.2052049551706503 1102 9 0.23094004634365461 1102 10-11 0.3258967046143155 1102 12-13 0.3258389322159019 1102 14-15 0.40562972527016683 1102 16-17 0.43095773896510536 1102 18-19 0.4855653371466815 1102 20-21 0.42841497498292114 1102 22-23 0.3564888341832173 1102 24-25 0.4182373541759219 1102 26-27 0.49193649963292785 1102 28-29 0.4206946409921528 1102 30-31 0.39469947522626825 1102 32-33 0.21613843868508553 1102 34-35 0.2782785958966514 1102 36-37 0.2962632807218242 1102 38-39 0.42230445393662563 1102 40-41 0.4026000845684834 1102 42-43 0.3450615745284793 1102 44-45 0.4629936391791105 1102 46-47 0.47167110472311435 1102 48-49 0.4561319854443937 1102 50-51 0.21526667873517624 1102 52-53 0.0637774278401011 1102 54-55 0.1829797333646539 1102 56-57 0.16559177846342976 1102 58-59 0.29273771429251383 1102 60-61 0.3137114835691648 1102 62-63 0.2481599312454108 1102 64-65 0.3322579974811575 1102 66-67 0.43711800398551404 1102 68-69 0.21591342741028186 1102 70-71 0.260287475472591 1102 72-73 0.09502622924664905 1102 74-75 0.02687078622681227 1102 76-77 0.11372338544905958 1102 78-79 0.18526387606531358 1102 80-81 0.2853778892680552 1102 82-83 0.2308805507990428 1102 84-85 0.0750985338429011 1102 86-87 0.043932639607437096 1102 88-89 0.1913999911345634 1102 90-91 0.04583469783895566 1102 92-93 0.06883032354998164 1102 94-95 0.03296889826465943 1102 96-97 0.04954324999601667 1102 98-99 0.04471202857520851 1102 100-101 -0.28980025784236574 1103 1 0.2841094955950574 1103 2 0.2790333556290676 1103 3 0.25156546581742845 1103 4 0.14613421873492882 1103 5 0.16489092080372814 1103 6 0.16487843024636106 1103 7 0.23314744209982052 1103 8 0.20836056054183416 1103 9 0.2567686228621895 1103 10-11 0.27427443900960924 1103 12-13 0.27933710248142063 1103 14-15 0.43125516290950117 1103 16-17 0.440356512214116 1103 18-19 0.3962530649356353 1103 20-21 0.34631163395456355 1103 22-23 0.4227180470881038 1103 24-25 0.39753337032805547 1103 26-27 0.4700279838828578 1103 28-29 0.3906474068727377 1103 30-31 0.38255607550784276 1103 32-33 0.49896036184861003 1103 34-35 0.42925823994587375 1103 36-37 0.54933532945536 1103 38-39 0.5764794898756236 1103 40-41 0.6054251253323457 1103 42-43 0.6485940631405782 1103 44-45 0.7032763016098045 1103 46-47 0.6353161733474408 1103 48-49 0.7509220299073149 1103 50-51 0.7439678517941672 1103 52-53 0.9331380970849708 1103 54-55 0.8155005606669192 1103 56-57 0.809888079158462 1103 58-59 0.897985328462255 1103 60-61 0.7708448253150735 1103 62-63 0.7189862685351187 1103 64-65 0.8430837409422409 1103 66-67 0.949312423480908 1103 68-69 0.8748721488972286 1103 70-71 0.585733576863884 1103 72-73 0.5485617612305056 1103 74-75 0.66975169636817 1103 76-77 0.6377266811979858 1103 78-79 0.6966351500999117 1103 80-81 0.6712612464782488 1103 82-83 0.6975338068356791 1103 84-85 0.6262536542540289 1103 86-87 0.8394979768402457 1103 88-89 0.8527525843099859 1103 90-91 0.7650450047246444 1103 92-93 0.811078608251222 1103 94-95 0.8480052331543213 1103 96-97 0.7271074639029891 1103 98-99 0.7710709435513188 1103 100-101 0.1622801960406548 1104 1 0.23192426680831701 1104 2 0.16551518827274236 1104 3 0.229995856986001 1104 4 0.11094084324646758 1104 5 0.15848963876148758 1104 6 0.11059193046136073 1104 7 0.10361013810119601 1104 8 0.19453424248914786 1104 9 0.184006616052514 1104 10-11 0.26441239483836654 1104 12-13 0.20390226317140758 1104 14-15 0.4206855987239706 1104 16-17 0.4296221341667419 1104 18-19 0.36550153346379943 1104 20-21 0.2638986405880033 1104 22-23 0.31283861451398565 1104 24-25 0.3760938925440911 1104 26-27 0.41164628024233707 1104 28-29 0.3339562789528401 1104 30-31 0.40318370286026806 1104 32-33 0.33803215010485843 1104 34-35 0.3109925686477837 1104 36-37 0.27282442998148326 1104 38-39 0.3771643109747984 1104 40-41 0.3361187581221756 1104 42-43 0.332512046273834 1104 44-45 0.24826230864506016 1104 46-47 0.24622857104370865 1104 48-49 0.21488249713840446 1104 50-51 0.26028747883642467 1104 52-53 0.318341506151846 1104 54-55 0.4823524843177225 1104 56-57 0.44553505698354456 1104 58-59 0.465090041894328 1104 60-61 0.24763854481192737 1104 62-63 0.28760165821158523 1104 64-65 0.22955415363482956 1104 66-67 0.09643269248419983 1104 68-69 0.08604057907691853 1104 70-71 -0.08123376818547356 1104 72-73 -0.04116047078248286 1104 74-75 0.010045184411293206 1104 76-77 -0.10016499845123761 1104 78-79 -0.11257139827862161 1104 80-81 0.15801931974921857 1104 82-83 0.028855630926138076 1104 84-85 -0.06284546199214702 1104 86-87 0.11572943998035612 1104 88-89 0.039368119673067525 1104 90-91 -0.11652267728376131 1104 92-93 -0.048996490850925056 1104 94-95 0.070746162399967 1104 96-97 0.0027372413079334024 1104 98-99 0.09431284678250407 1104 100-101 -0.12033760451436493 1105 1 0.24192676452067374 1105 2 0.21437405205281124 1105 3 0.21048542461322484 1105 4 0.1718493685549447 1105 5 0.11840157772873994 1105 6 0.12985677075404567 1105 7 0.15540721502193122 1105 8 0.1654661701797977 1105 9 0.16901322845477296 1105 10-11 0.2134163820195667 1105 12-13 0.32938683404015023 1105 14-15 0.44652914127501475 1105 16-17 0.2992279446512782 1105 18-19 0.3146658846263293 1105 20-21 0.26682805082645444 1105 22-23 0.3438070022251054 1105 24-25 0.3741449388846192 1105 26-27 0.41597143953262616 1105 28-29 0.2922971666286003 1105 30-31 0.46305166288370714 1105 32-33 0.5020880152785381 1105 34-35 0.4671105278931904 1105 36-37 0.47181976986730945 1105 38-39 0.4601875494807288 1105 40-41 0.5527415540288416 1105 42-43 0.4807837298004216 1105 44-45 0.32724554854166144 1105 46-47 0.1154863112177793 1105 48-49 0.19200575493068328 1105 50-51 0.16222586012140994 1105 52-53 0.19931995495776533 1105 54-55 0.2871566192987629 1105 56-57 0.31814874802177684 1105 58-59 0.4287807377868589 1105 60-61 0.3695240624461462 1105 62-63 0.44462364883942485 1105 64-65 0.1859508892341566 1105 66-67 0.12338406694178872 1105 68-69 0.110288795666726 1105 70-71 0.13831359191751247 1105 72-73 0.37324748825041 1105 74-75 0.2617997406467687 1105 76-77 0.28671601858806994 1105 78-79 0.3608335669672549 1105 80-81 0.3815707555070169 1105 82-83 0.4300211351799419 1105 84-85 0.3956986482393958 1105 86-87 0.47370519394226207 1105 88-89 0.45264561711644546 1105 90-91 0.4299237911563658 1105 92-93 0.4825153204653603 1105 94-95 0.5929502828517705 1105 96-97 0.5358834581362188 1105 98-99 0.4831169867429601 1105 100-101 0.274699123594484 1106 1 0.1706688277357813 1106 2 0.15352351663085528 1106 3 0.19593040320562238 1106 4 0.09732086154947694 1106 5 0.05403820762543177 1106 6 0.03272577669835641 1106 7 0.10801923697161442 1106 8 0.09985728087576007 1106 9 0.07292843204525212 1106 10-11 0.07451296017836029 1106 12-13 0.058669243208903765 1106 14-15 0.1938073394151658 1106 16-17 0.04955150326957636 1106 18-19 0.2977020810358155 1106 20-21 0.18891151469397727 1106 22-23 0.13306390278279423 1106 24-25 0.12830856412650604 1106 26-27 0.13253841918398734 1106 28-29 0.327618188029561 1106 30-31 0.39268726215065186 1106 32-33 0.29230562124038073 1106 34-35 0.16060588454933367 1106 36-37 -0.3223045035798151 1106 38-39 -0.10278109450124617 1106 40-41 0.2114120066680627 1106 42-43 0.3587431388710485 1106 44-45 0.2525488120450987 1106 46-47 -0.08266235974737413 1106 48-49 -0.014915775697929234 1106 50-51 0.12078359419130891 1106 52-53 -0.05833477113208829 1106 54-55 -0.20994368500988259 1106 56-57 -0.021632493292351285 1106 58-59 0.043984135856412365 1106 60-61 -0.24021063085667294 1106 62-63 -0.052546229974119285 1106 64-65 -0.37713092810964 1106 66-67 -0.12839181026983582 1106 68-69 -0.8619078153245958 1106 70-71 -0.6073314201546047 1106 72-73 -0.45242810161982305 1106 74-75 -0.3120729905727977 1106 76-77 -0.30478971713236547 1106 78-79 -0.3602104074433221 1106 80-81 -0.9878383361097072 1106 82-83 -0.41828610968190816 1106 84-85 -0.2941100905283207 1106 86-87 -0.1172998764692359 1106 88-89 0.09092789736902418 1106 90-91 -0.06675583009944219 1106 92-93 -0.21261846986445931 1106 94-95 -0.23401027072165093 1106 96-97 -0.2606802522568188 1106 98-99 -0.5110936360551221 1106 100-101 -0.5257221891710309 1107 1 0.11107489548161098 1107 2 0.1728015537761678 1107 3 0.13432213059337528 1107 4 0.10529095913953768 1107 5 0.047609771248048105 1107 6 0.14118018861277903 1107 7 0.08465434321534815 1107 8 0.11037984585096439 1107 9 0.2186517765774454 1107 10-11 0.19826763242991063 1107 12-13 0.2033926895489202 1107 14-15 0.2517505306538723 1107 16-17 0.23693242448486984 1107 18-19 0.22615663838997335 1107 20-21 0.32745740820300284 1107 22-23 0.3487437608336137 1107 24-25 0.2738464729939736 1107 26-27 0.26100170292237124 1107 28-29 0.34845606635467874 1107 30-31 0.30407810235600863 1107 32-33 0.3122199572352642 1107 34-35 0.23291488581465813 1107 36-37 0.29849774200971524 1107 38-39 0.22786160369675912 1107 40-41 0.1811350894087269 1107 42-43 0.20294082056164342 1107 44-45 0.32620313087809194 1107 46-47 0.22411367760154377 1107 48-49 0.11570365214214462 1107 50-51 0.23705539197189296 1107 52-53 0.3141363954400447 1107 54-55 0.20211617798587866 1107 56-57 0.08348044066347171 1107 58-59 -0.004287312868804349 1107 60-61 -0.023450882758289993 1107 62-63 0.02627119952396839 1107 64-65 0.045371508005956684 1107 66-67 -0.09685320329164071 1107 68-69 -0.04402745405228714 1107 70-71 -0.051700720734906724 1107 72-73 -0.014374521620695191 1107 74-75 -0.019157360164253845 1107 76-77 -0.1042755876619097 1107 78-79 -0.16672465326556463 1107 80-81 -0.04691232130393885 1107 82-83 -0.16881332872869947 1107 84-85 -0.07206360798836187 1107 86-87 -0.04055496322404295 1107 88-89 -4.91885344008125E-4 1107 90-91 0.11837077519165362 1107 92-93 -0.050602238789807075 1107 94-95 -0.05004354733348393 1107 96-97 0.007001583774421505 1107 98-99 -0.10198256372794035 1107 100-101 -0.4122537423219832 1108 1 0.1774108158056542 1108 2 0.1606038506418983 1108 3 0.17566526209755295 1108 4 0.12172857807561854 1108 5 0.08400090307745245 1108 6 0.07523983459061867 1108 7 0.152043386222374 1108 8 0.19032182730903457 1108 9 0.15781103363553228 1108 10-11 0.16739757047506743 1108 12-13 0.23437229045929797 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2316 54-55 -0.7827861840325028 2316 56-57 -0.6191146930577744 2316 58-59 -0.6270933460076904 2316 60-61 -0.49011980325929727 2316 62-63 -0.551755684337202 2316 64-65 -0.499447318147034 2316 66-67 -0.5474562761443273 2316 68-69 -0.5407086047348848 2316 70-71 -0.7406520178791745 2316 72-73 -0.6477718544581847 2316 74-75 -0.36578221194334404 2316 76-77 -0.3930667373553298 2316 78-79 -0.437934643010621 2316 80-81 -0.511756105541874 2316 82-83 -0.367877533582206 2316 84-85 -0.32374506231253264 2316 86-87 -0.2062583504866211 2316 88-89 0.022988607719813814 2316 90-91 0.029477983830350496 2316 92-93 0.08364881206045283 2316 94-95 -0.06998105977652713 2316 96-97 0.014097170766792289 2316 98-99 0.12930262895611477 2316 100-101 0.41457125307643494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 16.0 4 43.0 5 88.0 6 257.0 7 671.0 8 1547.0 9 2645.0 10 4513.0 11 6971.0 12 9923.0 13 13685.0 14 17666.0 15 23401.0 16 29127.0 17 36291.0 18 34882.0 19 29714.0 20 28648.0 21 30011.0 22 32044.0 23 35956.0 24 41691.0 25 49184.0 26 58302.0 27 70331.0 28 84430.0 29 101055.0 30 120119.0 31 143219.0 32 172950.0 33 213705.0 34 273760.0 35 362473.0 36 477691.0 37 540754.0 38 272443.0 39 12830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.456905474971364 19.093363320134102 15.125042678986217 23.324688525908318 2 15.99650357522041 20.475577406876322 38.78026765299785 24.74765136490542 3 19.218586906201686 23.65313306608765 30.196131848270618 26.932148179440045 4 13.435792196024957 16.8759797722503 35.884123976723984 33.80410405500076 5 13.714593727806564 37.52427483300026 34.025846109495376 14.7352853296978 6 33.06919048497695 35.99255335586528 16.658040920539626 14.28021523861814 7 29.191955104116506 32.845161106435704 19.806063224468918 18.156820564978872 8 27.332364249507584 34.44984991201739 19.315400782167657 18.90238505630737 9 26.62163277123471 16.429211126762468 19.556381627010737 37.39277447499209 10-11 25.215876173288986 26.394303107218775 27.011936532510063 21.377884186982175 12-13 26.380066875784756 23.56078300773017 27.285920232100075 22.773229884385 14-15 22.496845977177028 25.20225499505707 25.626881725269236 26.674017302496665 16-17 22.240398794495725 28.37894477872336 26.831995967651203 22.548660459129717 18-19 22.443531365463112 26.41748011202969 29.19086000939081 21.948128513116387 20-21 22.68202199490256 25.67721107875831 29.59337482692253 22.047392099416598 22-23 22.459732766884336 25.75931318059012 29.567587596327083 22.21336645619846 24-25 22.419449637402984 25.98239924585341 29.522411828964497 22.07573928777911 26-27 21.738848648875713 26.367687036047982 29.208258570922773 22.68520574415353 28-29 22.03668117292146 26.971928071778205 28.942153496277427 22.049237259022906 30-31 22.099656620297655 26.314001161100435 28.842589884024967 22.743752334576943 32-33 21.59799822684662 26.354444659463045 28.962540259732467 23.085016853957868 34-35 22.726785818769383 26.501471022874746 28.628535593352566 22.14320756500331 36-37 22.887155337376484 25.802855169541814 28.68427700581031 22.625712487271386 38-39 22.163983543618652 26.234228601123576 28.7158873928421 22.885900462415677 40-41 22.727561857069777 26.029100914072945 28.876843935509328 22.366493293347954 42-43 22.418613191464594 26.228175290141053 28.496513986956028 22.85669753143832 44-45 22.31771883033665 26.41503886311749 28.913280335741092 22.353961970804768 46-47 22.91304197813111 26.835356258316345 27.851844184521962 22.399757579030585 48-49 22.493950726737864 26.921373698824947 27.697420826901524 22.887254747535664 50-51 22.79696193720757 27.047399600065408 27.72719840266183 22.428440060065196 52-53 23.245620746194547 26.546401263709313 27.827572084985352 22.38040590511079 54-55 22.3791908000042 27.001015587848347 28.08529437796369 22.53449923418376 56-57 22.572632532714078 27.1003241780414 27.656902322050858 22.67014096719366 58-59 22.410168479573482 26.59695563666257 27.71764257608283 23.275233307681116 60-61 21.805631187097685 26.97948872577478 28.207209923658393 23.00767016346914 62-63 22.231848054856744 26.96024206093827 28.244023107998846 22.56388677620614 64-65 21.819402378305725 27.105169597170153 27.90199352243969 23.17343450208443 66-67 21.660119845456517 27.138492833499722 28.002298200517995 23.199089120525766 68-69 22.656745516220454 26.501598853170133 28.066773796929745 22.774881833679668 70-71 22.250161339149443 26.778668489226504 27.47181927855073 23.499350893073323 72-73 22.17029773075371 26.93053949166603 27.60898817747735 23.290174600102908 74-75 22.20540076716636 27.325328640927438 27.448324280050223 23.020946311855976 76-77 22.25499505707244 26.769170533251124 27.809525523957824 23.166308885718614 78-79 22.299038866861984 27.134632145680605 27.65771239212192 22.908616595335495 80-81 22.59980354023615 27.06045728200257 27.337655168109915 23.002084009651373 82-83 22.369212375235428 27.179628730936063 27.501648276229346 22.949510617599163 84-85 22.168160186048212 27.37695811109816 27.455013269977087 22.99986843287654 86-87 22.594279219867435 27.33954860706901 26.940760378425022 23.125411794638538 88-89 22.186635783972154 27.444099497681172 26.76509525456096 23.604169463785716 90-91 22.037145799626305 27.35647651215628 27.087150337651583 23.519227350565828 92-93 22.244539363551933 26.903776417347245 27.332067511877767 23.519616707223058 94-95 21.849276517201695 26.593198289611536 27.638549026055458 23.918976167131305 96-97 22.186094097139403 26.080445958575417 27.65624226495592 24.077217679329262 98-99 21.78738441307472 26.54129776584074 27.199990039133905 24.47132778195064 100-101 21.78092404993032 26.139011024453616 27.77791778988882 24.30214713572724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 640.0 1 449.0 2 367.0 3 524.0 4 475.0 5 491.0 6 879.5 7 1860.5 8 2865.5 9 3179.5 10 2748.5 11 2254.0 12 2240.5 13 2384.5 14 2641.5 15 2951.0 16 3281.5 17 3573.5 18 3934.0 19 4471.5 20 5202.5 21 6221.0 22 7558.5 23 9123.0 24 11142.0 25 13998.5 26 17823.5 27 22540.0 28 27292.5 29 32279.5 30 38138.0 31 46838.0 32 61797.0 33 66438.0 34 62020.0 35 64843.0 36 70843.5 37 77421.5 38 83179.0 39 87601.5 40 92555.0 41 99386.0 42 103582.5 43 106157.0 44 109839.5 45 117650.5 46 122289.0 47 124396.0 48 125663.5 49 122720.5 50 121189.0 51 118706.0 52 115103.0 53 117055.0 54 122755.5 55 116938.5 56 105719.5 57 95975.5 58 88584.0 59 77846.5 60 60385.0 61 50214.5 62 44474.0 63 32671.0 64 21130.0 65 15687.0 66 11941.0 67 9393.5 68 7356.5 69 5708.0 70 4496.0 71 3041.0 72 2168.5 73 1776.5 74 1353.5 75 990.5 76 720.5 77 479.5 78 310.0 79 200.0 80 108.0 81 56.5 82 32.5 83 20.5 84 11.5 85 7.5 86 4.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.900596901631991E-4 2 0.04926426135860752 3 9.600830471835814E-4 4 0.0 5 0.08850765591223642 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8001557134692152E-4 26-27 0.007950687734489034 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009900856424080683 36-37 9.000778567346076E-5 38-39 0.0033902932603670213 40-41 0.0048004152359179065 42-43 3.300285474693561E-4 44-45 1.500129761224346E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.500648806121729E-5 68-69 9.300804519590945E-4 70-71 1.2001038089794767E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014101219755508852 82-83 5.25045416428521E-4 84-85 0.004365377605162847 86-87 0.002190189451387545 88-89 7.500648806121729E-5 90-91 0.0029252530343874747 92-93 9.450817495713379E-4 94-95 3.000259522448692E-5 96-97 0.0 98-99 4.500389283673038E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3333045.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.97473620402988 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93149177157403 34.98869078707639 2 14.371369122904465 13.789252851241637 3 5.2003963824844535 7.484629338182487 4 2.4906604560526886 4.779551134117459 5 1.354826714978159 3.2498727126624773 6 0.836379538051183 2.4075052622672324 7 0.5431627072261545 1.824066131652903 8 0.38644484223534 1.4831671490918705 9 0.27608532306988226 1.192060848967377 >10 1.4474948102595901 12.274219670037793 >50 0.09755493888597691 3.220851990819165 >100 0.053491900358755354 5.002719555089019 >500 0.005758925039776864 1.8762012020562917 >1k 0.0043191937782287365 4.502270256302303 >5k 5.007760902294186E-4 1.5977861086869443 >10k+ 6.259701127867733E-5 0.32715500174862455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10894 0.3268482723755605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9184 0.27554383454168785 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6934 0.20803799528659228 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6933 0.2080079926913678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6673 0.20020731793300123 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6284 0.1885363083906758 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6154 0.18463597101149248 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5672 0.1701747201132898 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5371 0.16114393895071924 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4686 0.1405921612219457 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4475 0.13426161362957895 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4323 0.12970121915545696 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4286 0.12859112313215093 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4038 0.12115047951647819 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3924 0.11773018366088668 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3572 0.10716927014186726 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3507 0.10521910145227563 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3465 0.10395899245284716 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3444 0.10332893795313294 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3371 0.1011387485017454 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.000778567346076E-5 2 0.0 0.0 0.0 0.0 9.000778567346076E-5 3 0.0 0.0 0.0 0.0 9.000778567346076E-5 4 0.0 0.0 0.0 0.0 9.000778567346076E-5 5 0.0 0.0 0.0 0.0 9.000778567346076E-5 6 0.0 0.0 0.0 0.0 9.000778567346076E-5 7 0.0 0.0 0.0 0.0 9.000778567346076E-5 8 0.0 0.0 0.0 0.0 9.000778567346076E-5 9 0.0 0.0 0.0 3.000259522448692E-5 9.000778567346076E-5 10-11 0.0 0.0 0.0 3.000259522448692E-5 1.0500908328570422E-4 12-13 0.0 0.0 0.0 3.000259522448692E-5 2.850246546326257E-4 14-15 0.0 0.0 0.0 4.500389283673038E-5 3.300285474693561E-4 16-17 1.500129761224346E-5 0.0 0.0 6.000519044897384E-5 3.300285474693561E-4 18-19 4.500389283673038E-5 0.0 0.0 7.50064880612173E-5 3.6003114269384304E-4 20-21 6.000519044897384E-5 0.0 0.0 1.0500908328570422E-4 4.5003892836730376E-4 22-23 9.000778567346076E-5 0.0 0.0 3.450298450815996E-4 4.5003892836730376E-4 24-25 1.8001557134692152E-4 0.0 0.0 8.400726662856337E-4 4.800415235917907E-4 26-27 1.8001557134692152E-4 0.0 0.0 0.0013801193803263984 5.400467140407645E-4 28-29 1.8001557134692152E-4 0.0 0.0 0.0039903451648567595 5.700493092652514E-4 30-31 2.4002076179589535E-4 0.0 0.0 0.013036127625039567 5.700493092652514E-4 32-33 2.4002076179589535E-4 0.0 0.0 0.03238780154483363 6.000519044897384E-4 34-35 2.7002335702038227E-4 0.0 0.0 0.06146031631736145 6.300544997142253E-4 36-37 3.300285474693561E-4 0.0 0.0 0.10875940768876508 6.300544997142253E-4 38-39 3.900337379183299E-4 0.0 0.0 0.18420093338073745 6.300544997142253E-4 40-41 3.900337379183299E-4 0.0 0.0 0.2614876186790157 6.300544997142253E-4 42-43 3.900337379183299E-4 0.0 0.0 0.34628995408102803 6.300544997142253E-4 44-45 4.2003633314281687E-4 0.0 0.0 0.4509990114144874 6.600570949387122E-4 46-47 4.950428212040341E-4 0.0 0.0 0.5781350086782506 6.900596901631991E-4 48-49 5.400467140407645E-4 0.0 0.0 0.7114215379630338 7.200622853876861E-4 50-51 5.400467140407645E-4 0.0 0.0 0.8591843194436318 7.50064880612173E-4 52-53 5.400467140407645E-4 0.0 0.0 1.0012616091291897 8.400726662856337E-4 54-55 5.400467140407645E-4 0.0 0.0 1.152624702036726 8.700752615101206E-4 56-57 5.400467140407645E-4 0.0 0.0 1.3305550930155459 8.850765591223641E-4 58-59 5.400467140407645E-4 0.0 0.0 1.5198114636916094 9.000778567346075E-4 60-61 5.400467140407645E-4 0.0 0.0 1.7355901285461193 9.600830471835814E-4 62-63 5.400467140407645E-4 0.0 0.0 1.9414979395717729 0.001050090832857042 64-65 5.400467140407645E-4 0.0 0.0 2.1552964331414666 0.0010950947256937725 66-67 5.400467140407645E-4 0.0 0.0 2.4109335457517074 0.001110096023306016 68-69 5.400467140407645E-4 0.0 0.0 2.6942480524565378 0.001110096023306016 70-71 5.400467140407645E-4 0.0 0.0 2.9885885129063663 0.0011400986185305028 72-73 5.55048011653008E-4 0.0 0.0 3.307741119606846 0.0013201141898774244 74-75 5.700493092652514E-4 0.0 0.0 3.6259186419625298 0.0014851284636121023 76-77 5.700493092652514E-4 0.0 0.0 3.970108414377844 0.001500129761224346 78-79 5.700493092652514E-4 0.0 0.0 4.3569768784999905 0.0015151310588365895 80-81 6.000519044897384E-4 0.0 0.0 4.761501869911747 0.0015901375468978067 82-83 6.000519044897384E-4 0.0 0.0 5.1847634820411965 0.0018451596063059454 84-85 6.000519044897384E-4 0.0 0.0 5.62329641513991 0.001860160903918189 86-87 6.000519044897384E-4 0.0 0.0 6.075975571886968 0.0019051647967549194 88-89 6.000519044897384E-4 0.0 0.0 6.550211593302821 0.0019501686895916496 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 55.247116 1 GTATCAA 11100 0.0 42.55336 1 TATCAAC 14605 0.0 32.21086 2 ATCAACG 14545 0.0 32.16234 3 TCAACGC 14760 0.0 31.62943 4 CAACGCA 15170 0.0 30.805922 5 AGGTAAG 2180 0.0 29.89315 2 AACGCAG 15770 0.0 29.635962 6 TAAGGTG 2540 0.0 28.07526 5 ACGCAGA 17980 0.0 25.861198 7 AAGGTAA 2405 0.0 25.712002 1 TGGTATC 2725 0.0 25.660107 2 GTAAGGT 2655 0.0 25.605766 4 CGCAGAG 18135 0.0 25.58778 8 GGTAAGG 2670 0.0 24.749693 3 GTGGTAT 3305 0.0 23.891544 1 AGGACCG 1160 0.0 23.360552 5 GTACATG 13110 0.0 22.169003 1 GCAGAGT 20525 0.0 22.076023 9 GGACCGA 1020 0.0 21.88534 6 >>END_MODULE