FastQCFastQC Report
Thu 26 May 2016
SRR938357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317993
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114100.3438825820307638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86300.2600969923685794No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66750.2011758312931944No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA66630.2008141668773864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.1941233751849386No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63690.19195338869009065No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56830.17127823958640057No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52630.1586199850331209No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA50220.1513565580156438No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49510.1492167102221132No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49000.14767963645492924No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA45620.13749275540967085No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42450.12793878709207646No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38750.11678746760466342No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35180.10602795123437572No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34790.10485254188299976No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG34660.10446073876587442No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33650.10141672993282384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.053.8082581
GTATCAA112350.042.3370741
ATCAACG145950.032.2373
TATCAAC146750.032.1903152
TCAACGC148000.031.7904744
CAACGCA151050.031.1800235
AACGCAG156650.030.1821866
GTGTAAG13200.027.4411951
ACGCAGA175000.026.6595527
GTACATG127800.026.5902271
CGCAGAG175350.026.4166818
TGGTATC28050.026.2612742
TACATGG126800.026.1234472
TAAGGTG24600.026.0808755
ACATGGG132250.024.1129513
AGGTAAG22100.024.0847822
GTGGTAT31550.024.0193441
GCAGAGT193500.023.2997689
TAAGGTA13950.022.82574
GTAAGGT27400.021.8546314
Produced by FastQC (version 0.11.5)