FastQCFastQC Report
Thu 26 May 2016
SRR938357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317993
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112050.3377041482607106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91370.275377313936467No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA69470.20937355805150884No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67150.20238137934588768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66790.20129638609846373No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62880.18951215388338674No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC60960.18372552323045888No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56430.17007269153370727No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG55030.1658532733492807No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47880.144304101907388No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45260.13640776216224687No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42960.1294758608592604No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42250.1273360130657298No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40350.12160965981543662No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38040.1146476198111328No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35960.10837876993712765No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35860.10807738292395433No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35560.10717322188443436No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG34640.10440046136323976No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG34540.10409907435006643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.058.366511
GTATCAA109100.043.1620451
ATCAACG142900.032.5388073
TCAACGC143950.032.367524
TATCAAC145000.032.1148762
CAACGCA148000.031.4817875
AACGCAG153650.030.354896
TAAGGTG24400.030.202085
GTGTAAG12550.027.6675511
AGGTAAG23450.027.585932
TGGTATC24650.027.0148582
ACGCAGA175500.026.4944767
CGCAGAG175950.026.3727258
GTAAGGT27850.025.607144
GGTAAGG28550.024.8127633
GTGGTAT28500.024.5337541
AAGGTAA26500.024.2314551
GTACATG124750.024.0591551
TACATGG121350.023.9493082
TAAGGTA13650.023.3365764
Produced by FastQC (version 0.11.5)